Timely and accurate evaluation of clinical parameters associated with endothelial dysfunctionis critical in diagnosing and treating atherosclerosis, which represents a severe health problem, accountingfor at least 30% of deaths globally. A critical early event in the pathogenesis of atherosclerosis is the oxidativemodification of low-density lipoprotein (LDL). Oxidized LDL (OxLDL) represents numerouschanges in lipid and apolipoprotein B (apo B) fractions of LDLs generated by lipid peroxidation. Anotherindicator of perturbed vascular homeostasis is homocysteine (Hcy), an amino acid containing sulfhydrylgroup,an intermediate methionine and cysteine biosynthesis product. The total level of Hcy in plasmacorrelates better than cholesterol with the risk of cardiovascular disease. In addition, nitric oxide (NO)plays an essential role in regulating vascular physiological homeostasis due to its involvement in intravascularfree radical and oxidant reactions. Reduced NO decreases oxidative stress... in the vascular wall,which reduces the rate of LDL oxidation and the expression of redox-sensitive genes involved in atherogenesis.Endothelial dysfunction is typically associated with increased levels of OxLDL, decreased nitricoxide (NO), and hyperhomocysteinemia. Thus, OxLDL, Hcy, and NO are representative parameters ofoxidative stress and endothelial dysfunction. Considering the important role of oxLDL, Hcy and NO inoxidative stress, atherogenesis and accompanying endothelial dysfunction, the challenge of the presentwork was to systematically present available methods for reliable measurement of these parameters andassess their potential for the use in the clinical setting. Here we present a comprehensive overview ofanalytical methods for measuring OxLDL, HCy, and NO in biological samples and discuss their advantagesand potential problems regarding their application in clinical settings.
Source:
Current Analytical Chemistry, 2022, 18, 10, 1040-1056
TY - JOUR
AU - Mačvanin, Mirjana T.
AU - Stanimirović, Julijana
AU - Isenović, Esma R.
PY - 2022
UR - https://vinar.vin.bg.ac.rs/handle/123456789/10545
AB - Timely and accurate evaluation of clinical parameters associated with endothelial dysfunctionis critical in diagnosing and treating atherosclerosis, which represents a severe health problem, accountingfor at least 30% of deaths globally. A critical early event in the pathogenesis of atherosclerosis is the oxidativemodification of low-density lipoprotein (LDL). Oxidized LDL (OxLDL) represents numerouschanges in lipid and apolipoprotein B (apo B) fractions of LDLs generated by lipid peroxidation. Anotherindicator of perturbed vascular homeostasis is homocysteine (Hcy), an amino acid containing sulfhydrylgroup,an intermediate methionine and cysteine biosynthesis product. The total level of Hcy in plasmacorrelates better than cholesterol with the risk of cardiovascular disease. In addition, nitric oxide (NO)plays an essential role in regulating vascular physiological homeostasis due to its involvement in intravascularfree radical and oxidant reactions. Reduced NO decreases oxidative stress in the vascular wall,which reduces the rate of LDL oxidation and the expression of redox-sensitive genes involved in atherogenesis.Endothelial dysfunction is typically associated with increased levels of OxLDL, decreased nitricoxide (NO), and hyperhomocysteinemia. Thus, OxLDL, Hcy, and NO are representative parameters ofoxidative stress and endothelial dysfunction. Considering the important role of oxLDL, Hcy and NO inoxidative stress, atherogenesis and accompanying endothelial dysfunction, the challenge of the presentwork was to systematically present available methods for reliable measurement of these parameters andassess their potential for the use in the clinical setting. Here we present a comprehensive overview ofanalytical methods for measuring OxLDL, HCy, and NO in biological samples and discuss their advantagesand potential problems regarding their application in clinical settings.
T2 - Current Analytical Chemistry
T1 - Methods for Measurements of Oxidized LDL, Homocysteine and Nitric Oxide as Clinical Parameters of Oxidative Stress and Endothelial Dysfunction
VL - 18
IS - 10
SP - 1040
EP - 1056
DO - 10.2174/1573411018666220827142613
ER -
@article{
author = "Mačvanin, Mirjana T. and Stanimirović, Julijana and Isenović, Esma R.",
year = "2022",
abstract = "Timely and accurate evaluation of clinical parameters associated with endothelial dysfunctionis critical in diagnosing and treating atherosclerosis, which represents a severe health problem, accountingfor at least 30% of deaths globally. A critical early event in the pathogenesis of atherosclerosis is the oxidativemodification of low-density lipoprotein (LDL). Oxidized LDL (OxLDL) represents numerouschanges in lipid and apolipoprotein B (apo B) fractions of LDLs generated by lipid peroxidation. Anotherindicator of perturbed vascular homeostasis is homocysteine (Hcy), an amino acid containing sulfhydrylgroup,an intermediate methionine and cysteine biosynthesis product. The total level of Hcy in plasmacorrelates better than cholesterol with the risk of cardiovascular disease. In addition, nitric oxide (NO)plays an essential role in regulating vascular physiological homeostasis due to its involvement in intravascularfree radical and oxidant reactions. Reduced NO decreases oxidative stress in the vascular wall,which reduces the rate of LDL oxidation and the expression of redox-sensitive genes involved in atherogenesis.Endothelial dysfunction is typically associated with increased levels of OxLDL, decreased nitricoxide (NO), and hyperhomocysteinemia. Thus, OxLDL, Hcy, and NO are representative parameters ofoxidative stress and endothelial dysfunction. Considering the important role of oxLDL, Hcy and NO inoxidative stress, atherogenesis and accompanying endothelial dysfunction, the challenge of the presentwork was to systematically present available methods for reliable measurement of these parameters andassess their potential for the use in the clinical setting. Here we present a comprehensive overview ofanalytical methods for measuring OxLDL, HCy, and NO in biological samples and discuss their advantagesand potential problems regarding their application in clinical settings.",
journal = "Current Analytical Chemistry",
title = "Methods for Measurements of Oxidized LDL, Homocysteine and Nitric Oxide as Clinical Parameters of Oxidative Stress and Endothelial Dysfunction",
volume = "18",
number = "10",
pages = "1040-1056",
doi = "10.2174/1573411018666220827142613"
}
Mačvanin, M. T., Stanimirović, J.,& Isenović, E. R.. (2022). Methods for Measurements of Oxidized LDL, Homocysteine and Nitric Oxide as Clinical Parameters of Oxidative Stress and Endothelial Dysfunction. in Current Analytical Chemistry, 18(10), 1040-1056.
https://doi.org/10.2174/1573411018666220827142613
Mačvanin MT, Stanimirović J, Isenović ER. Methods for Measurements of Oxidized LDL, Homocysteine and Nitric Oxide as Clinical Parameters of Oxidative Stress and Endothelial Dysfunction. in Current Analytical Chemistry. 2022;18(10):1040-1056.
doi:10.2174/1573411018666220827142613 .
Mačvanin, Mirjana T., Stanimirović, Julijana, Isenović, Esma R., "Methods for Measurements of Oxidized LDL, Homocysteine and Nitric Oxide as Clinical Parameters of Oxidative Stress and Endothelial Dysfunction" in Current Analytical Chemistry, 18, no. 10 (2022):1040-1056,
https://doi.org/10.2174/1573411018666220827142613 . .
