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dc.creatorStanković, Aleksandra
dc.creatorKolaković, Ana
dc.creatorŽivković, Maja
dc.creatorĐurić, Tamara
dc.creatorBundalo, Maja M.
dc.creatorKončar, Igor
dc.creatorDavidovic, Lazar
dc.creatorAlavantić, Dragan
dc.date.accessioned2018-03-01T16:51:20Z
dc.date.available2018-03-01T16:51:20Z
dc.date.issued2016
dc.identifier.issn0021-9150 (print)
dc.identifier.issn1879-1484 (electronic)
dc.identifier.urihttp://vinar.vin.bg.ac.rs/handle/123456789/1045
dc.description.abstractBackground and Aims: The principal biologic effects of the renin-angiotensin system are mediated by activation of the AT1R receptor. The microRNA miR-155 regulates AT1R expression, with both its, and AT1Rs activity, linked to atherosclerosis. Target sites for miR-155 lie within the 3 UTR of the human AT1R gene, and include the AT1R A1166C polymorphism. Thus far, only levels of circulating miR-155 have been investigated with respect to A1166C genotypes. We hypothesized that the A1166C polymorphism could correlate with different, ultra-sonographically defined plaque phenotypes, as well as with an altered expression of AT1R mRNA and protein in human carotid plaques (CP), and altered expression of miR-155 in patients with advanced atherosclerosis. Methods: Our study cohort comprised 411 patients with advanced carotid atherosclerosis (298 hyperechoic; 113 hypoechoic plaques). PCR analyses identified A1166C genotypes; quantitative real-time PCR determined AT1R and miR-155 expression levels, with AT1R protein expression evaluated by western blot. Results: Genotypes containing the C allele bore a significant association with the hypoechoic plaque phenotype (adjusted OR 1.87, 95% CI 1.16-3.00, p = 0.01). The expression of AT1R mRNA and miR-155 were significantly up-regulated in the CPs of CC genotype carriers compared to the AA/AC genotypes (p = 0.032, p = 0.015, respectively). AT1R protein expression was also significantly higher for CC genotypes (p LT 0.01). Conclusion: Our results indicate that the AT1R A1166C polymorphism impacts an ultrasonographicallydefined human plaque phenotype, with intra-plaque AT1R and miR-155 expression altered in advanced carotid atherosclerosis. Validation and replication of these data should contribute to an improved personalized therapy with which to prevent carotid atherosclerosis. (C) 2016 Elsevier Ireland Ltd. All rights reserved.en
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175085/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41028/RS//
dc.rightsrestrictedAccessen
dc.sourceAtherosclerosisen
dc.subjectAngiotensin receptor type 1en
dc.subjectPolymorphismen
dc.subjectmRNAen
dc.subjectProteinen
dc.subjectmiR-155en
dc.subjectCarotid plaqueen
dc.titleAngiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaquesen
dc.typearticleen
dcterms.abstractДавидовиц, Лазар; Станковић Aлександра; Концар, Игор; Колаковић Aна; Живковић Маја; Ђурић Тамара; Бундало Маја; Aлавантић Драган;
dc.citation.volume248
dc.citation.spage132
dc.citation.epage139
dc.identifier.wos000375039200017
dc.identifier.doi10.1016/j.atherosclerosis.2016.02.032
dc.citation.rankM21
dc.identifier.pmid27016615
dc.identifier.scopus2-s2.0-84962613008


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