Cymbopogon martinii essential oil nanoemulsions: Physico-chemical characterization, antibacterial and antibiofilm potential against Enterococcus faecalis
Само за регистроване кориснике
2022
Аутори
Marinković, Jelena![](/themes/MirageVinar/images/orcid.png)
Bošković, Marko
![](/themes/MirageVinar/images/orcid.png)
Tasić, Gvozden
![](/themes/MirageVinar/images/orcid.png)
Vasilijević, Bojana
Marković, Dejan
![](/themes/MirageVinar/images/orcid.png)
Marković, Tatjana
![](/themes/MirageVinar/images/orcid.png)
Nikolić, Biljana
![](/themes/MirageVinar/images/orcid.png)
Чланак у часопису (Објављена верзија)
![](/themes/MirageVinar//images/creativecommons/arr.png)
Метаподаци
Приказ свих података о документуАпстракт
Cymbopogon martinii is an aromatic crop cultivated for the production of its essential oil (EO) which has different applications. Bearing in mind that C. martinii EO (CMEO) has already been proved for high antibacterial potential the aim of this study was to formulate stable CMEO nanoemulsions, being capable to combat Enterococcus faecalis biofilm, especially within infected tooth’s root canal. Ten emulsions with variable CMEO content (2.5–10 % v/v) and Tween 80 (T80, 2.5–15 % v/v) were formulated (designated as ECMEO:T80) and screened for volume-weighted mean diameter (D4,3) and stability, using dynamic light scattering measurements. Chemical composition of stable nanoemulsions was monitored by UV–VIS and FTIR, while their antibacterial activity was estimated in microdilution assay. Antibiofilm properties of selected nanoemulsions were tested in vitro (cristal violet assay) and ex vivo (within root canals of the extracted teeth). Results showed that formulations containing 10 % of T80... and CMEO volume ≤ 6 %, as well as those having constant 2.5 % of CMEO and variable T80 volume (≤ 10%), namely E2.5:2.5, E2.5:5, E2.5:7.5, E2.5:10, E4:10, and E6:10, were stable and nanosized (D4,3 <100 nm). FTIR and UV–VIS analyses confirmed successful encapsulation of the CMEO by T80 and showed that nanoemulsification did not affect CMEO composition. Evaluation of antibacterial potential showed that the most efficient were E6:10, E2.5:2.5 and E4:10 (MIC values 0.37–1.97 mg mL−1). Analysis of dependency of determined MICs on mean diameter and CMEO volume showed that antibacterial potential increased with both parameters. E6:10, E2.5:2.5 and E4:10 also reduced in vitro biofilm (inhibitory range 17.6–58.4 %), as well as intracanal biofilm (reduction ~2Log CFU). Presented results suggest E6:10, E4:10 and E2.5:2.5 as the good candidates for further research.
Кључне речи:
Antibiofilm potential / Nanoemulsions / Root canal infection / ToothИзвор:
Industrial Crops and Products, 2022, 187, 115478-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-MESTD-inst-2020-200017)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200003 (Институт за проучавање лековитог биља 'Др Јосиф Панчић', Београд) (RS-MESTD-inst-2020-200003)
Институција/група
VinčaTY - JOUR AU - Marinković, Jelena AU - Bošković, Marko AU - Tasić, Gvozden AU - Vasilijević, Bojana AU - Marković, Dejan AU - Marković, Tatjana AU - Nikolić, Biljana PY - 2022 UR - https://vinar.vin.bg.ac.rs/handle/123456789/10396 AB - Cymbopogon martinii is an aromatic crop cultivated for the production of its essential oil (EO) which has different applications. Bearing in mind that C. martinii EO (CMEO) has already been proved for high antibacterial potential the aim of this study was to formulate stable CMEO nanoemulsions, being capable to combat Enterococcus faecalis biofilm, especially within infected tooth’s root canal. Ten emulsions with variable CMEO content (2.5–10 % v/v) and Tween 80 (T80, 2.5–15 % v/v) were formulated (designated as ECMEO:T80) and screened for volume-weighted mean diameter (D4,3) and stability, using dynamic light scattering measurements. Chemical composition of stable nanoemulsions was monitored by UV–VIS and FTIR, while their antibacterial activity was estimated in microdilution assay. Antibiofilm properties of selected nanoemulsions were tested in vitro (cristal violet assay) and ex vivo (within root canals of the extracted teeth). Results showed that formulations containing 10 % of T80 and CMEO volume ≤ 6 %, as well as those having constant 2.5 % of CMEO and variable T80 volume (≤ 10%), namely E2.5:2.5, E2.5:5, E2.5:7.5, E2.5:10, E4:10, and E6:10, were stable and nanosized (D4,3 <100 nm). FTIR and UV–VIS analyses confirmed successful encapsulation of the CMEO by T80 and showed that nanoemulsification did not affect CMEO composition. Evaluation of antibacterial potential showed that the most efficient were E6:10, E2.5:2.5 and E4:10 (MIC values 0.37–1.97 mg mL−1). Analysis of dependency of determined MICs on mean diameter and CMEO volume showed that antibacterial potential increased with both parameters. E6:10, E2.5:2.5 and E4:10 also reduced in vitro biofilm (inhibitory range 17.6–58.4 %), as well as intracanal biofilm (reduction ~2Log CFU). Presented results suggest E6:10, E4:10 and E2.5:2.5 as the good candidates for further research. T2 - Industrial Crops and Products T1 - Cymbopogon martinii essential oil nanoemulsions: Physico-chemical characterization, antibacterial and antibiofilm potential against Enterococcus faecalis VL - 187 SP - 115478 DO - 10.1016/j.indcrop.2022.115478 ER -
@article{ author = "Marinković, Jelena and Bošković, Marko and Tasić, Gvozden and Vasilijević, Bojana and Marković, Dejan and Marković, Tatjana and Nikolić, Biljana", year = "2022", abstract = "Cymbopogon martinii is an aromatic crop cultivated for the production of its essential oil (EO) which has different applications. Bearing in mind that C. martinii EO (CMEO) has already been proved for high antibacterial potential the aim of this study was to formulate stable CMEO nanoemulsions, being capable to combat Enterococcus faecalis biofilm, especially within infected tooth’s root canal. Ten emulsions with variable CMEO content (2.5–10 % v/v) and Tween 80 (T80, 2.5–15 % v/v) were formulated (designated as ECMEO:T80) and screened for volume-weighted mean diameter (D4,3) and stability, using dynamic light scattering measurements. Chemical composition of stable nanoemulsions was monitored by UV–VIS and FTIR, while their antibacterial activity was estimated in microdilution assay. Antibiofilm properties of selected nanoemulsions were tested in vitro (cristal violet assay) and ex vivo (within root canals of the extracted teeth). Results showed that formulations containing 10 % of T80 and CMEO volume ≤ 6 %, as well as those having constant 2.5 % of CMEO and variable T80 volume (≤ 10%), namely E2.5:2.5, E2.5:5, E2.5:7.5, E2.5:10, E4:10, and E6:10, were stable and nanosized (D4,3 <100 nm). FTIR and UV–VIS analyses confirmed successful encapsulation of the CMEO by T80 and showed that nanoemulsification did not affect CMEO composition. Evaluation of antibacterial potential showed that the most efficient were E6:10, E2.5:2.5 and E4:10 (MIC values 0.37–1.97 mg mL−1). Analysis of dependency of determined MICs on mean diameter and CMEO volume showed that antibacterial potential increased with both parameters. E6:10, E2.5:2.5 and E4:10 also reduced in vitro biofilm (inhibitory range 17.6–58.4 %), as well as intracanal biofilm (reduction ~2Log CFU). Presented results suggest E6:10, E4:10 and E2.5:2.5 as the good candidates for further research.", journal = "Industrial Crops and Products", title = "Cymbopogon martinii essential oil nanoemulsions: Physico-chemical characterization, antibacterial and antibiofilm potential against Enterococcus faecalis", volume = "187", pages = "115478", doi = "10.1016/j.indcrop.2022.115478" }
Marinković, J., Bošković, M., Tasić, G., Vasilijević, B., Marković, D., Marković, T.,& Nikolić, B.. (2022). Cymbopogon martinii essential oil nanoemulsions: Physico-chemical characterization, antibacterial and antibiofilm potential against Enterococcus faecalis. in Industrial Crops and Products, 187, 115478. https://doi.org/10.1016/j.indcrop.2022.115478
Marinković J, Bošković M, Tasić G, Vasilijević B, Marković D, Marković T, Nikolić B. Cymbopogon martinii essential oil nanoemulsions: Physico-chemical characterization, antibacterial and antibiofilm potential against Enterococcus faecalis. in Industrial Crops and Products. 2022;187:115478. doi:10.1016/j.indcrop.2022.115478 .
Marinković, Jelena, Bošković, Marko, Tasić, Gvozden, Vasilijević, Bojana, Marković, Dejan, Marković, Tatjana, Nikolić, Biljana, "Cymbopogon martinii essential oil nanoemulsions: Physico-chemical characterization, antibacterial and antibiofilm potential against Enterococcus faecalis" in Industrial Crops and Products, 187 (2022):115478, https://doi.org/10.1016/j.indcrop.2022.115478 . .