Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis
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2022
Authors
Đorđević, Ana D.
Živković, Maja

Končar, Igor

Stanković, Aleksandra

Kuveljić, Jovana
Đurić, Tamara

Article (Published version)

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ObjectivesGalectin-3 affects a variety of biological processes. It is encoded by LGALS-3, located in unique haplotype block in Caucasians. Most of the studies regarding the gal-3 role in atherosclerosis are focused exclusively on protein/mRNA levels. Genetic analyses of LGALS-3 are scarce. We sought to thoroughly examine the genetic background of gal-3 and to analyze tag variants that cover more than 80% variability of the LGALS-3 containing hap-block in association with carotid plaque presence (CPP). According to Tagger server, rs4040064 G/T, rs11628437 G/A and rs7159490 C/T cover 82% (r2 > 0.8) of the genetic variance of this hap-block. Our aims were to investigate possible association of rs4040064, rs11628437 and rs7159490 haplotypes with CPP in patients with advanced carotid atherosclerosis (CA) and to analyze their possible effect on LGALS-3 mRNA expression in carotid plaques.Materials and methodsStudy group consisted of 468 patients and 296 controls. Rs4040064, rs11628437, rs7159...490 and LGALS-3 mRNA expression were detected by TaqMan® technology.ResultsWe have found that haplotype TAC was associated with the cerebrovascular insult (CVI) occurrence (OR = 1.68, 95% CI = 1.09-2.58, p = 0.02), compared to the referent haplotype. OR was adjusted for hypertension, age and BMI. TAC also showed higher, but not statistically significant, LGALS-3 expression in carotid plaques.ConclusionsOur results suggest that rs4040064, rs11628437 and rs7159490 bear no association with CPP, neither they affect LGALS-3 mRNA in carotid plaques. However, we showed a significant association of haplotype TAC with the CVI occurrence in CA patients from Serbia. Replication and validation of our results are required.
Keywords:
3 / Carotid atherosclerosis / Galectin-3 / rs11628437 / rs4040064 / rs7159490 / Tag variantsSource:
Journal of Stroke and Cerebrovascular Diseases, 2022, 31, 1, 106212-Funding / projects:
DOI: 10.1016/j.jstrokecerebrovasdis.2021.106212
ISSN: 1052-3057
PubMed: 34814004
WoS: 000722029100001
Scopus: 2-s2.0-85119288672
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VinčaTY - JOUR AU - Đorđević, Ana D. AU - Živković, Maja AU - Končar, Igor AU - Stanković, Aleksandra AU - Kuveljić, Jovana AU - Đurić, Tamara PY - 2022 UR - https://vinar.vin.bg.ac.rs/handle/123456789/10046 AB - ObjectivesGalectin-3 affects a variety of biological processes. It is encoded by LGALS-3, located in unique haplotype block in Caucasians. Most of the studies regarding the gal-3 role in atherosclerosis are focused exclusively on protein/mRNA levels. Genetic analyses of LGALS-3 are scarce. We sought to thoroughly examine the genetic background of gal-3 and to analyze tag variants that cover more than 80% variability of the LGALS-3 containing hap-block in association with carotid plaque presence (CPP). According to Tagger server, rs4040064 G/T, rs11628437 G/A and rs7159490 C/T cover 82% (r2 > 0.8) of the genetic variance of this hap-block. Our aims were to investigate possible association of rs4040064, rs11628437 and rs7159490 haplotypes with CPP in patients with advanced carotid atherosclerosis (CA) and to analyze their possible effect on LGALS-3 mRNA expression in carotid plaques.Materials and methodsStudy group consisted of 468 patients and 296 controls. Rs4040064, rs11628437, rs7159490 and LGALS-3 mRNA expression were detected by TaqMan® technology.ResultsWe have found that haplotype TAC was associated with the cerebrovascular insult (CVI) occurrence (OR = 1.68, 95% CI = 1.09-2.58, p = 0.02), compared to the referent haplotype. OR was adjusted for hypertension, age and BMI. TAC also showed higher, but not statistically significant, LGALS-3 expression in carotid plaques.ConclusionsOur results suggest that rs4040064, rs11628437 and rs7159490 bear no association with CPP, neither they affect LGALS-3 mRNA in carotid plaques. However, we showed a significant association of haplotype TAC with the CVI occurrence in CA patients from Serbia. Replication and validation of our results are required. T2 - Journal of Stroke and Cerebrovascular Diseases T2 - Journal of Stroke and Cerebrovascular DiseasesJournal of Stroke and Cerebrovascular Diseases T1 - Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis VL - 31 IS - 1 SP - 106212 DO - 10.1016/j.jstrokecerebrovasdis.2021.106212 ER -
@article{ author = "Đorđević, Ana D. and Živković, Maja and Končar, Igor and Stanković, Aleksandra and Kuveljić, Jovana and Đurić, Tamara", year = "2022", abstract = "ObjectivesGalectin-3 affects a variety of biological processes. It is encoded by LGALS-3, located in unique haplotype block in Caucasians. Most of the studies regarding the gal-3 role in atherosclerosis are focused exclusively on protein/mRNA levels. Genetic analyses of LGALS-3 are scarce. We sought to thoroughly examine the genetic background of gal-3 and to analyze tag variants that cover more than 80% variability of the LGALS-3 containing hap-block in association with carotid plaque presence (CPP). According to Tagger server, rs4040064 G/T, rs11628437 G/A and rs7159490 C/T cover 82% (r2 > 0.8) of the genetic variance of this hap-block. Our aims were to investigate possible association of rs4040064, rs11628437 and rs7159490 haplotypes with CPP in patients with advanced carotid atherosclerosis (CA) and to analyze their possible effect on LGALS-3 mRNA expression in carotid plaques.Materials and methodsStudy group consisted of 468 patients and 296 controls. Rs4040064, rs11628437, rs7159490 and LGALS-3 mRNA expression were detected by TaqMan® technology.ResultsWe have found that haplotype TAC was associated with the cerebrovascular insult (CVI) occurrence (OR = 1.68, 95% CI = 1.09-2.58, p = 0.02), compared to the referent haplotype. OR was adjusted for hypertension, age and BMI. TAC also showed higher, but not statistically significant, LGALS-3 expression in carotid plaques.ConclusionsOur results suggest that rs4040064, rs11628437 and rs7159490 bear no association with CPP, neither they affect LGALS-3 mRNA in carotid plaques. However, we showed a significant association of haplotype TAC with the CVI occurrence in CA patients from Serbia. Replication and validation of our results are required.", journal = "Journal of Stroke and Cerebrovascular Diseases, Journal of Stroke and Cerebrovascular DiseasesJournal of Stroke and Cerebrovascular Diseases", title = "Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis", volume = "31", number = "1", pages = "106212", doi = "10.1016/j.jstrokecerebrovasdis.2021.106212" }
Đorđević, A. D., Živković, M., Končar, I., Stanković, A., Kuveljić, J.,& Đurić, T.. (2022). Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis. in Journal of Stroke and Cerebrovascular Diseases, 31(1), 106212. https://doi.org/10.1016/j.jstrokecerebrovasdis.2021.106212
Đorđević AD, Živković M, Končar I, Stanković A, Kuveljić J, Đurić T. Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis. in Journal of Stroke and Cerebrovascular Diseases. 2022;31(1):106212. doi:10.1016/j.jstrokecerebrovasdis.2021.106212 .
Đorđević, Ana D., Živković, Maja, Končar, Igor, Stanković, Aleksandra, Kuveljić, Jovana, Đurić, Tamara, "Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis" in Journal of Stroke and Cerebrovascular Diseases, 31, no. 1 (2022):106212, https://doi.org/10.1016/j.jstrokecerebrovasdis.2021.106212 . .