Raw Dataset file for relative mRNA quantification of Orosomucoid-like 3 (ORMDL3) and Ikaros family zinc finger 3 (IKZF3) mRNAs and plasma sCD25 protein (soluble form of Interleukin-2 Receptor Alpha – IL2RA protein) quantification experimental data in a group of relapsing-remitting multiple sclerosis (RRMS) patients and healthy controls from the Serbian population. 

Raw Table S1. Ct values for expression analysis, relative expression values for target mRNAs and genotypes.

Peripheral blood mononuclar cells (PBMC) RNA was extracted with a TRI reagent-based protocol (TRI Reagent, Ambion, Austin, Texas, USA) from 3ml of blood samples collected in EDTA vacuateners, within 30 minutes of blood collection. 500ng of total RNA entered the DNA digestion and reverse transcription experiments. DNA digestion and reverse transcription was performed according to the Thermo Scientific™ RevertAid RT Reverse Transcription Kit (Thermo Fisher Scientific Inc, Waltham, Massachusetts, USA) manufacturer’s protocol with the oligodT primer (Oligo (dT)18 Primer, Thermo Fisher Scientific Inc, Waltham, Massachusetts, USA). TaqMan® based quantitative real-time PCR was performed in duplicate for every sample on the Applied Biosystems 7500 RT-PCR System with the following assays: Hs00918021_m1 for ORMDL3, Hs00232635_m1 for IKZF3 and Hs99999904_m1 for the internal reference control, PPIA (Applied Biosystems, Foster City, California, USA). Cycle thresholds (Ct) were determined with the SDS v1.4.0 software (Applied Biosystems, Foster City, California, USA).  Relative expression of target mRNAs was calculated with the 2^-(duplicate average CtTarget-duplicate average CtPPIA) formula. The genotyping real-time PCR was performed on the Applied Biosystems 7500 RT-PCR System with the 1.4.0v SDS software (Applied Biosystems, Foster City, California, USA), according to the manufacturer’s protocols. Only samples with allele calling quality greater than 95% were included in downstream analysis.  The following assays were used for genotyping (Applied Biosystems, Foster City, California, USA): C__16095542_10 for IL2RA rs2104286 and C__31651862_10 for IKZF3 rs12946510. 

Raw Table 2. 450 nm optical densities for ELISA, sCD25 plasma concentration and IL2RA rs2104286 genotype

Within 30 minutes after blood sample collection, EDTA vacutainers with fresh whole blood were centrifuged at 1000g, 4oC, 15 minutes, for plasma extraction. Concentration levels of plasma sCD25 were obtained with the “sandwich” ELISA method. ELISA was performed with the FineTest® Human CD25 (Interleukin 2 Receptor Alpha) ELISA Kit (Wuhan Fine Biotech, Wuhan, PRC), according to the manufacturer’s protocol, on 4x diluted plasma samples. The plate optical density was read on the spectrophotometer Perkin Elmer Wallac 1420 Victor2 Microplate Reader (PerkinElmer, Waltham, Massachusetts, USA). Concentration levels were calculated from the optical density values, using four parameter logistic regression of the MyAssays Online data analysis tools (www.myassays.com; Four Parameter Logistic Curve).