TY  - CONF
AU  - Mitrović, Nataša
AU  - Zarić, Marina
AU  - Martinović, Jelena
AU  - Guševac Stojanović, Ivana
AU  - Petrović, Snježana
AU  - Paunović, Marija
AU  - Vučić, Vesna
AU  - Grković, Ivana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11050
AB  - It is increasingly apparent that the prevention/treatment of neurodegenerative disorders is not only achieved through pharmacological therapy but also through the consumption of natural products. Flaxseed oil (or linseed oil, FSO) derived from the seeds of the flax (Linum usitatissimum L.) gained worldwide awareness as a neuroprotective agent due to its high content of omega-3 polyunsaturated fatty acids (n-3 PUFAs). Thus, the aim of this study was to examine the preventive effects of dietary FSO in trimethyltin (TMT) - induced hippocampal neurodegeneration and gliosis in female Wistar rats. Animals were continuously treated with FSO (1 ml/kg, orally) for two weeks, then received a single dose of TMT (8 mg/kg, i.p.), and application of FSO continued for twenty-one days. Data have convincingly shown that FSO continuous treatment ameliorated TMT-induced neuronal loss in the CA3 hippocampal region and ameliorated astrogliosis and microgliosis. FSO treatment elevated all tested n-3 fatty acids in the hippocampus: α-linolenic acid (ALA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA), and consequently increased total amount of n-3 PUFA. However, no changes in n-6 fatty acids due to FSO treatment were observed. Consequently, FSO lowered n-6/n-3 ratio compared to TMT, having a protective effect on fatty acid profile in hippocampus. These findings support beneficial neuroprotective properties of FSO against TMT-induced model of neurodegeneration and hint at a promising preventive use of FSO in hippocampal degeneration and dysfunction
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats
SP  - 81
ER  - 

@conference{
author = "Mitrović, Nataša and Zarić, Marina and Martinović, Jelena and Guševac Stojanović, Ivana and Petrović, Snježana and Paunović, Marija and Vučić, Vesna and Grković, Ivana",
year = "2023",
abstract = "It is increasingly apparent that the prevention/treatment of neurodegenerative disorders is not only achieved through pharmacological therapy but also through the consumption of natural products. Flaxseed oil (or linseed oil, FSO) derived from the seeds of the flax (Linum usitatissimum L.) gained worldwide awareness as a neuroprotective agent due to its high content of omega-3 polyunsaturated fatty acids (n-3 PUFAs). Thus, the aim of this study was to examine the preventive effects of dietary FSO in trimethyltin (TMT) - induced hippocampal neurodegeneration and gliosis in female Wistar rats. Animals were continuously treated with FSO (1 ml/kg, orally) for two weeks, then received a single dose of TMT (8 mg/kg, i.p.), and application of FSO continued for twenty-one days. Data have convincingly shown that FSO continuous treatment ameliorated TMT-induced neuronal loss in the CA3 hippocampal region and ameliorated astrogliosis and microgliosis. FSO treatment elevated all tested n-3 fatty acids in the hippocampus: α-linolenic acid (ALA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA), and consequently increased total amount of n-3 PUFA. However, no changes in n-6 fatty acids due to FSO treatment were observed. Consequently, FSO lowered n-6/n-3 ratio compared to TMT, having a protective effect on fatty acid profile in hippocampus. These findings support beneficial neuroprotective properties of FSO against TMT-induced model of neurodegeneration and hint at a promising preventive use of FSO in hippocampal degeneration and dysfunction",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats",
pages = "81"
}

Mitrović, N., Zarić, M., Martinović, J., Guševac Stojanović, I., Petrović, S., Paunović, M., Vučić, V.,& Grković, I.. (2023). Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 81.

Mitrović N, Zarić M, Martinović J, Guševac Stojanović I, Petrović S, Paunović M, Vučić V, Grković I. Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:81..

Mitrović, Nataša, Zarić, Marina, Martinović, Jelena, Guševac Stojanović, Ivana, Petrović, Snježana, Paunović, Marija, Vučić, Vesna, Grković, Ivana, "Dietary supplementation with flaxseed oil ameliorates trimethyltin (TMT)-induced neurodegeneration and gliosis in female Wistar rats" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):81.

TY  - CONF
AU  - Jovanović Macura, Irena
AU  - Đuričić, Ivana
AU  - Major, Tamara
AU  - Milanović, Desanka
AU  - Brkić, Marjana
AU  - Šobajić, Slađana
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11049
AB  - Mfsd2a is expressed mainly in the endothelial cells and is an essential regulator of blood vessel transcytosis. Therefore, decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Mfsd2a is also the main docosahexaenoic acid (DHA, C22:6n3) transporter. DHA, an omega-3 fatty acid, is one of the main structural lipids of the neuronal and vascular retina, crucial for the normal functioning of photoreceptors (PRs). However, the capacity of the retina to synthesize DHA is limited, and the maintenance of retinal DHA content relies on the uptake from bloodborne lipids. The currently recommended FO doses yielded low PUFAs tissue bioavailability, and supplementation with higher doses has been increasingly recommended. Nevertheless, the effects of higher FO doses on retinal Mfsd2a expression and blood vessels coverage are unknown. Western blot and qPCR analyses showed that high dose FO supplementation increased Mfsd2a expression in the retina. Immunohistochemical analyses of Mfsd2a expression on retinal blood vessels (labeled with 488-conjugated Lycopersicon esculentum, lectin) and subsequent ImageJ analyses revealed 1.32-fold increase in the Mfsd2a retinal blood vessel coverage. In the same time the pericyte blood vessel coverage (CD13+ cells) was not affected with FO supplementation, and the increase in Mfsd2a blood vessel expression is not the result of the increased pericyte coverage. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels that can serve either as prophylaxis in the healthy eye or as an adjuvant in developing targeted manipulations of the barrier during diseases.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice
SP  - 66
ER  - 

@conference{
author = "Jovanović Macura, Irena and Đuričić, Ivana and Major, Tamara and Milanović, Desanka and Brkić, Marjana and Šobajić, Slađana and Kanazir, Selma and Ivković, Sanja",
year = "2023",
abstract = "Mfsd2a is expressed mainly in the endothelial cells and is an essential regulator of blood vessel transcytosis. Therefore, decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Mfsd2a is also the main docosahexaenoic acid (DHA, C22:6n3) transporter. DHA, an omega-3 fatty acid, is one of the main structural lipids of the neuronal and vascular retina, crucial for the normal functioning of photoreceptors (PRs). However, the capacity of the retina to synthesize DHA is limited, and the maintenance of retinal DHA content relies on the uptake from bloodborne lipids. The currently recommended FO doses yielded low PUFAs tissue bioavailability, and supplementation with higher doses has been increasingly recommended. Nevertheless, the effects of higher FO doses on retinal Mfsd2a expression and blood vessels coverage are unknown. Western blot and qPCR analyses showed that high dose FO supplementation increased Mfsd2a expression in the retina. Immunohistochemical analyses of Mfsd2a expression on retinal blood vessels (labeled with 488-conjugated Lycopersicon esculentum, lectin) and subsequent ImageJ analyses revealed 1.32-fold increase in the Mfsd2a retinal blood vessel coverage. In the same time the pericyte blood vessel coverage (CD13+ cells) was not affected with FO supplementation, and the increase in Mfsd2a blood vessel expression is not the result of the increased pericyte coverage. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels that can serve either as prophylaxis in the healthy eye or as an adjuvant in developing targeted manipulations of the barrier during diseases.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice",
pages = "66"
}

Jovanović Macura, I., Đuričić, I., Major, T., Milanović, D., Brkić, M., Šobajić, S., Kanazir, S.,& Ivković, S.. (2023). The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 66.

Jovanović Macura I, Đuričić I, Major T, Milanović D, Brkić M, Šobajić S, Kanazir S, Ivković S. The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:66..

Jovanović Macura, Irena, Đuričić, Ivana, Major, Tamara, Milanović, Desanka, Brkić, Marjana, Šobajić, Slađana, Kanazir, Selma, Ivković, Sanja, "The high-dose fish oil (FO) supplementation increased Mfsd2a expression in the retina of healthy mice" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):66.

TY  - CONF
AU  - Zarić Kontić, Marina
AU  - Dragić, Milorad
AU  - Martinović, Jelena
AU  - Mihajlović, Katarina
AU  - Brkić, Željka
AU  - Mitrović, Nataša
AU  - Guševac Stojanović, Ivana
AU  - Grković, Ivana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11048
AB  - The benzodiazepine alprazolam (ALP) is commonly prescribed to treat anxiety, panic, and sleep disorders. However, ALP is often abused for prolonged periods of time, leading to severe side effects such as tolerance, dependence, and withdrawal syndrome. Previous literature data suggest that neuroadaptive changes at synaptic receptors, such as gammaaminobutyric acid receptor type A (GABAAR) and glutamatergic receptors, may be responsible for the occurrence and development of the aforementioned side effects. Therefore, the present study investigated the potential effects of prolonged ALP treatment (2 mg/kg, ip.) on the α1-subunit containing GABAAR and components of glutamatergic neurotransmission in the hippocampus of adult male Wistar rats. The study revealed behavioral changes consistent with a possible onset of tolerance and associated changes in the GABAergic and glutamatergic systems. The primary target of ALP, the α1-subunit containing GABAAR, was decreased indicating its potential downregulation by prolonged agonist (ALP) action. Considering studied glutamatergic components, an increase in NMDAR subunits, a decrease in vGlut1, and differential modulation of excitatory amino acid transporters 1 and 2 (EAAT1/2, in vivo and in vitro) were observed. These changes may all together indicate a compensatory mechanism due to the sustained suppression of glutamatergic neurons by enhanced inhibitory impulses from GABAergic neurons. The data presented provide valuable and, to our knowledge, the first information on components of glutamatergic neurotransmission after prolonged ALP treatment and their potential impact on the development of side effects. However, further research is needed to examine the observed changes in detail.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Long-term alprazolam treatment may cause tolerance development by modulating components of glutamatergic neurotransmission in the hippocampus of male Wistar rats
SP  - 60
ER  - 

@conference{
author = "Zarić Kontić, Marina and Dragić, Milorad and Martinović, Jelena and Mihajlović, Katarina and Brkić, Željka and Mitrović, Nataša and Guševac Stojanović, Ivana and Grković, Ivana",
year = "2023",
abstract = "The benzodiazepine alprazolam (ALP) is commonly prescribed to treat anxiety, panic, and sleep disorders. However, ALP is often abused for prolonged periods of time, leading to severe side effects such as tolerance, dependence, and withdrawal syndrome. Previous literature data suggest that neuroadaptive changes at synaptic receptors, such as gammaaminobutyric acid receptor type A (GABAAR) and glutamatergic receptors, may be responsible for the occurrence and development of the aforementioned side effects. Therefore, the present study investigated the potential effects of prolonged ALP treatment (2 mg/kg, ip.) on the α1-subunit containing GABAAR and components of glutamatergic neurotransmission in the hippocampus of adult male Wistar rats. The study revealed behavioral changes consistent with a possible onset of tolerance and associated changes in the GABAergic and glutamatergic systems. The primary target of ALP, the α1-subunit containing GABAAR, was decreased indicating its potential downregulation by prolonged agonist (ALP) action. Considering studied glutamatergic components, an increase in NMDAR subunits, a decrease in vGlut1, and differential modulation of excitatory amino acid transporters 1 and 2 (EAAT1/2, in vivo and in vitro) were observed. These changes may all together indicate a compensatory mechanism due to the sustained suppression of glutamatergic neurons by enhanced inhibitory impulses from GABAergic neurons. The data presented provide valuable and, to our knowledge, the first information on components of glutamatergic neurotransmission after prolonged ALP treatment and their potential impact on the development of side effects. However, further research is needed to examine the observed changes in detail.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Long-term alprazolam treatment may cause tolerance development by modulating components of glutamatergic neurotransmission in the hippocampus of male Wistar rats",
pages = "60"
}

Zarić Kontić, M., Dragić, M., Martinović, J., Mihajlović, K., Brkić, Ž., Mitrović, N., Guševac Stojanović, I.,& Grković, I.. (2023). Long-term alprazolam treatment may cause tolerance development by modulating components of glutamatergic neurotransmission in the hippocampus of male Wistar rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 60.

Zarić Kontić M, Dragić M, Martinović J, Mihajlović K, Brkić Ž, Mitrović N, Guševac Stojanović I, Grković I. Long-term alprazolam treatment may cause tolerance development by modulating components of glutamatergic neurotransmission in the hippocampus of male Wistar rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:60..

Zarić Kontić, Marina, Dragić, Milorad, Martinović, Jelena, Mihajlović, Katarina, Brkić, Željka, Mitrović, Nataša, Guševac Stojanović, Ivana, Grković, Ivana, "Long-term alprazolam treatment may cause tolerance development by modulating components of glutamatergic neurotransmission in the hippocampus of male Wistar rats" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):60.

TY  - CONF
AU  - Martinović, Jelena
AU  - Zarić Kontić, Marina
AU  - Guševac Stojanović, Ivana
AU  - Mitrović, Nataša
AU  - Grković, Ivana
AU  - Stojanović, Zoran
AU  - Drakulić, Dunja
AU  - Samardžić, Janko
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11047
AB  - Zaleplon, a member of Z-drugs, is a pyrazolopyrimidine hypnotic with sedative, anxiolytic, anticonvulsant and muscle relaxant properties. Zaleplon is approved for the short-term management of insomnia since acting as positive γ-aminobutyric acid (GABA) receptor allosteric modulator increases efficacy of inhibition on brain excitability. Importantly, for the proper functioning of the brain a balance between inhibitory (i.e., GABAergic) and excitatory (i.e., glutamatergic) system must be accomplished. This may be fulfilled by control of presynaptic elements (synthesis or degradation of glutamate and GABA neurotransmitters, their compartmentation, releasing and recycling) and regulation of expression and function of glutamate and GABA receptors. Hence, we aimed to investigate effects of prolonged zaleplon treatment on the expression of proteins involved in the gabaergic and glutamatergic signalization in the hippocampus of adult male Wistar rats. Five-day intraperitoneal administration increased level of components of GABAergic signalization (glutamate decarboxylase 67-GAD67, vesicular GABA transporter-VGAT and α1 subunit of GABA receptor-GABAAα1). This was accompanied by increased level of glutamatergic components (vesicular glutamate transporter 1-vGlut1 and subunits of glutamate N-Methyl-d-aspartate receptor-NMDAR, namely NR1, NR2A, NR2B), which clearly indicate maintenance of balance between main inhibitory and excitatory neurotransmitters. Given the importance of equilibrium of these systems for neuronal excitability, synaptic plasticity and cognitive functions, as well as its involvement in the mood, feeding behavior, reproductive functions, pain sensitivity, aging, etc., the current and prospective pharmaceuticals increasingly rely on GABA/glutamate balance
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats
SP  - 59
ER  - 

@conference{
author = "Martinović, Jelena and Zarić Kontić, Marina and Guševac Stojanović, Ivana and Mitrović, Nataša and Grković, Ivana and Stojanović, Zoran and Drakulić, Dunja and Samardžić, Janko",
year = "2023",
abstract = "Zaleplon, a member of Z-drugs, is a pyrazolopyrimidine hypnotic with sedative, anxiolytic, anticonvulsant and muscle relaxant properties. Zaleplon is approved for the short-term management of insomnia since acting as positive γ-aminobutyric acid (GABA) receptor allosteric modulator increases efficacy of inhibition on brain excitability. Importantly, for the proper functioning of the brain a balance between inhibitory (i.e., GABAergic) and excitatory (i.e., glutamatergic) system must be accomplished. This may be fulfilled by control of presynaptic elements (synthesis or degradation of glutamate and GABA neurotransmitters, their compartmentation, releasing and recycling) and regulation of expression and function of glutamate and GABA receptors. Hence, we aimed to investigate effects of prolonged zaleplon treatment on the expression of proteins involved in the gabaergic and glutamatergic signalization in the hippocampus of adult male Wistar rats. Five-day intraperitoneal administration increased level of components of GABAergic signalization (glutamate decarboxylase 67-GAD67, vesicular GABA transporter-VGAT and α1 subunit of GABA receptor-GABAAα1). This was accompanied by increased level of glutamatergic components (vesicular glutamate transporter 1-vGlut1 and subunits of glutamate N-Methyl-d-aspartate receptor-NMDAR, namely NR1, NR2A, NR2B), which clearly indicate maintenance of balance between main inhibitory and excitatory neurotransmitters. Given the importance of equilibrium of these systems for neuronal excitability, synaptic plasticity and cognitive functions, as well as its involvement in the mood, feeding behavior, reproductive functions, pain sensitivity, aging, etc., the current and prospective pharmaceuticals increasingly rely on GABA/glutamate balance",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats",
pages = "59"
}

Martinović, J., Zarić Kontić, M., Guševac Stojanović, I., Mitrović, N., Grković, I., Stojanović, Z., Drakulić, D.,& Samardžić, J.. (2023). Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 59.

Martinović J, Zarić Kontić M, Guševac Stojanović I, Mitrović N, Grković I, Stojanović Z, Drakulić D, Samardžić J. Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:59..

Martinović, Jelena, Zarić Kontić, Marina, Guševac Stojanović, Ivana, Mitrović, Nataša, Grković, Ivana, Stojanović, Zoran, Drakulić, Dunja, Samardžić, Janko, "Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):59.

TY  - CONF
AU  - Stanisavljević Ilić, Andrijana
AU  - Filipović, Dragana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11046
AB  - The alterations of gamma-aminobutyric acid (GABA) neurotransmission induced by psychosocial stress are implicated in the pathophysiology of major depressive disorder and anxiety. Olanzapine (Olz), an atypical antipsychotic, is used to treat psychiatric disorders. Since GABAergic signaling has been proposed as a potential therapeutic target for antipsychotics, we aimed to determine the susceptibility of different layers (1;2/3/4;5/6) in the retrosplenial cortex (RSC), including both granular c (RSGc) and dysgranular (RSD) subregions, in adult male rats exposed to chronic social isolation (CSIS) (six weeks), an animal model of depression, and/or treatment with Olz (7.5 mg/kg/day) (lasting 3 weeks of six-week CSIS), on the protein expression of parvalbumin interneurons, the largest class of GABAergic inhibitory neurons, and glutamate decarboxylase 67 (GAD67), as the main GABA-synthesizing enzyme. Results showed that CSIS decreased the number of GAD67 positive (GAD67+) cells in 5/6 layers of RSGc and increased in 1 and 2/3/4 layers of RSD, with no significant effect on PV positive (PV+) cells. Treatment with Olz in CSIS rats increased the number of GAD67+ cells in the 5/6 layers of the RSC and decreased in 1 and 2/3/4 layers of the RSD. Regarding PV+ cells, Olz treatment led to a decrease in 2/3/4 layers of RSD with no significant effect in RSGc. Overall, this study provided an insight into the neuroinhibitory transmission in the RSC, at the level of subregions and layers following CSIS and/or Olz treatment, and its involvement in the rat model of depression.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Olanzapine effects on parvalbumin/GAD67 protein expression in the layers of the retrosplenial cortex in chronically socially isolated rats
SP  - 58
ER  - 

@conference{
author = "Stanisavljević Ilić, Andrijana and Filipović, Dragana",
year = "2023",
abstract = "The alterations of gamma-aminobutyric acid (GABA) neurotransmission induced by psychosocial stress are implicated in the pathophysiology of major depressive disorder and anxiety. Olanzapine (Olz), an atypical antipsychotic, is used to treat psychiatric disorders. Since GABAergic signaling has been proposed as a potential therapeutic target for antipsychotics, we aimed to determine the susceptibility of different layers (1;2/3/4;5/6) in the retrosplenial cortex (RSC), including both granular c (RSGc) and dysgranular (RSD) subregions, in adult male rats exposed to chronic social isolation (CSIS) (six weeks), an animal model of depression, and/or treatment with Olz (7.5 mg/kg/day) (lasting 3 weeks of six-week CSIS), on the protein expression of parvalbumin interneurons, the largest class of GABAergic inhibitory neurons, and glutamate decarboxylase 67 (GAD67), as the main GABA-synthesizing enzyme. Results showed that CSIS decreased the number of GAD67 positive (GAD67+) cells in 5/6 layers of RSGc and increased in 1 and 2/3/4 layers of RSD, with no significant effect on PV positive (PV+) cells. Treatment with Olz in CSIS rats increased the number of GAD67+ cells in the 5/6 layers of the RSC and decreased in 1 and 2/3/4 layers of the RSD. Regarding PV+ cells, Olz treatment led to a decrease in 2/3/4 layers of RSD with no significant effect in RSGc. Overall, this study provided an insight into the neuroinhibitory transmission in the RSC, at the level of subregions and layers following CSIS and/or Olz treatment, and its involvement in the rat model of depression.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Olanzapine effects on parvalbumin/GAD67 protein expression in the layers of the retrosplenial cortex in chronically socially isolated rats",
pages = "58"
}

Stanisavljević Ilić, A.,& Filipović, D.. (2023). Olanzapine effects on parvalbumin/GAD67 protein expression in the layers of the retrosplenial cortex in chronically socially isolated rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 58.

Stanisavljević Ilić A, Filipović D. Olanzapine effects on parvalbumin/GAD67 protein expression in the layers of the retrosplenial cortex in chronically socially isolated rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:58..

Stanisavljević Ilić, Andrijana, Filipović, Dragana, "Olanzapine effects on parvalbumin/GAD67 protein expression in the layers of the retrosplenial cortex in chronically socially isolated rats" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):58.

TY  - CONF
AU  - Lukić, Iva
AU  - Mitić, Miloš
AU  - Pajović, Milica
AU  - Glavonić, Emilija
AU  - Živanović, Ana
AU  - Aleksić, Minja
AU  - Ivković, Sanja
AU  - Elliot, Evan
AU  - Adžić, Miroslav
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11045
AB  - There is accumulating evidence demonstrating effects of gastrointestinal microbiota on brain function and behavior, including depressive behavior. We have demonstrated that antidepressants, the main drugs used for alleviating depression, affect gut microbiota composition as well, and in this way partly contribute to improvement of depressive symptoms. Specifically, our results showed that several types of antidepressants reduced abundance of bacterial genera Ruminococcus, while supplementation with R. flavefaciens diminished antidepressant-induced decrease of depressive behavior. Treatment with R. flavefaciens affected cortical gene networks, up-regulating genes involved in mitochondrial oxidative phosphorylation, while down-regulating genes involved in neuronal plasticity, suggesting a mechanism for microbial regulation of antidepressant treatment efficiency. In further studies, we are aiming to delineate the role of gut microbiota in conveying the long-term effects of adolescent stress on development of anxiety and depressive behavior.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - The role of gut microbiota in depressive behavior and the effects of antidepressants
SP  - 44
ER  - 

@conference{
author = "Lukić, Iva and Mitić, Miloš and Pajović, Milica and Glavonić, Emilija and Živanović, Ana and Aleksić, Minja and Ivković, Sanja and Elliot, Evan and Adžić, Miroslav",
year = "2023",
abstract = "There is accumulating evidence demonstrating effects of gastrointestinal microbiota on brain function and behavior, including depressive behavior. We have demonstrated that antidepressants, the main drugs used for alleviating depression, affect gut microbiota composition as well, and in this way partly contribute to improvement of depressive symptoms. Specifically, our results showed that several types of antidepressants reduced abundance of bacterial genera Ruminococcus, while supplementation with R. flavefaciens diminished antidepressant-induced decrease of depressive behavior. Treatment with R. flavefaciens affected cortical gene networks, up-regulating genes involved in mitochondrial oxidative phosphorylation, while down-regulating genes involved in neuronal plasticity, suggesting a mechanism for microbial regulation of antidepressant treatment efficiency. In further studies, we are aiming to delineate the role of gut microbiota in conveying the long-term effects of adolescent stress on development of anxiety and depressive behavior.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "The role of gut microbiota in depressive behavior and the effects of antidepressants",
pages = "44"
}

Lukić, I., Mitić, M., Pajović, M., Glavonić, E., Živanović, A., Aleksić, M., Ivković, S., Elliot, E.,& Adžić, M.. (2023). The role of gut microbiota in depressive behavior and the effects of antidepressants. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 44.

Lukić I, Mitić M, Pajović M, Glavonić E, Živanović A, Aleksić M, Ivković S, Elliot E, Adžić M. The role of gut microbiota in depressive behavior and the effects of antidepressants. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:44..

Lukić, Iva, Mitić, Miloš, Pajović, Milica, Glavonić, Emilija, Živanović, Ana, Aleksić, Minja, Ivković, Sanja, Elliot, Evan, Adžić, Miroslav, "The role of gut microbiota in depressive behavior and the effects of antidepressants" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):44.

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Lukić, Iva
AU  - Mitić, Miloš
AU  - Glavonić, Emilija
AU  - Dragićević, Nina
AU  - Ivković, Sanja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11044
AB  - Major depressive disorder (MDD) afflicts approximately 5 % of the world population, and about 30–50 % of patients who receive classical antidepressant medications do not achieve complete remission (treatment resistant depressive patients). Emerging evidence suggests that targeting opioid receptors mu (MOP), kappa (KOP), delta (DOP), and the nociceptin/orphanin FQ receptor (NOP) may yield effective therapeutics for stress-related psychiatric disorders. As depression and pain exhibit significant overlap in their clinical manifestations and molecular mechanisms involved, it is not a surprise that opioids, historically used to alleviate pain, emerged as promising and effective therapeutic options in the treatment of depression. The opioid signaling is dysregulated in depression and numerous preclinical studies and clinical trials strongly suggest that opioid modulation can serve as either an adjuvant or even an alternative to classical monoaminergic antidepressants. Importantly, some classical antidepressants require the opioid receptor modulation to exert their antidepressant effects. Finally, ketamine, a well-known anesthetic whose extremely efficient antidepressant effects were recently discovered, was shown to mediate its antidepressant effects via the endogenous opioid system. Thus, although opioid system modulation is a promising therapeutical venue in the treatment of depression further research is warranted to fully understand the benefits and weaknesses of such approach.
T2  - Life Sciences
T2  - Life SciencesLife Sciences
T1  - Contribution of the opioid system to depression and to the therapeutic effects of classical antidepressants and ketamine
VL  - 326
SP  - 121803
DO  - 10.1016/j.lfs.2023.121803
ER  - 

@article{
author = "Adžić, Miroslav and Lukić, Iva and Mitić, Miloš and Glavonić, Emilija and Dragićević, Nina and Ivković, Sanja",
year = "2023",
abstract = "Major depressive disorder (MDD) afflicts approximately 5 % of the world population, and about 30–50 % of patients who receive classical antidepressant medications do not achieve complete remission (treatment resistant depressive patients). Emerging evidence suggests that targeting opioid receptors mu (MOP), kappa (KOP), delta (DOP), and the nociceptin/orphanin FQ receptor (NOP) may yield effective therapeutics for stress-related psychiatric disorders. As depression and pain exhibit significant overlap in their clinical manifestations and molecular mechanisms involved, it is not a surprise that opioids, historically used to alleviate pain, emerged as promising and effective therapeutic options in the treatment of depression. The opioid signaling is dysregulated in depression and numerous preclinical studies and clinical trials strongly suggest that opioid modulation can serve as either an adjuvant or even an alternative to classical monoaminergic antidepressants. Importantly, some classical antidepressants require the opioid receptor modulation to exert their antidepressant effects. Finally, ketamine, a well-known anesthetic whose extremely efficient antidepressant effects were recently discovered, was shown to mediate its antidepressant effects via the endogenous opioid system. Thus, although opioid system modulation is a promising therapeutical venue in the treatment of depression further research is warranted to fully understand the benefits and weaknesses of such approach.",
journal = "Life Sciences, Life SciencesLife Sciences",
title = "Contribution of the opioid system to depression and to the therapeutic effects of classical antidepressants and ketamine",
volume = "326",
pages = "121803",
doi = "10.1016/j.lfs.2023.121803"
}

Adžić, M., Lukić, I., Mitić, M., Glavonić, E., Dragićević, N.,& Ivković, S.. (2023). Contribution of the opioid system to depression and to the therapeutic effects of classical antidepressants and ketamine. in Life Sciences, 326, 121803.
https://doi.org/10.1016/j.lfs.2023.121803

Adžić M, Lukić I, Mitić M, Glavonić E, Dragićević N, Ivković S. Contribution of the opioid system to depression and to the therapeutic effects of classical antidepressants and ketamine. in Life Sciences. 2023;326:121803.
doi:10.1016/j.lfs.2023.121803 .

Adžić, Miroslav, Lukić, Iva, Mitić, Miloš, Glavonić, Emilija, Dragićević, Nina, Ivković, Sanja, "Contribution of the opioid system to depression and to the therapeutic effects of classical antidepressants and ketamine" in Life Sciences, 326 (2023):121803,
https://doi.org/10.1016/j.lfs.2023.121803 . .

TY  - CONF
AU  - Grković, Ivana
AU  - Dragić, Milorad
AU  - Mitrović, Nataša
AU  - Zarić Kontić, Marina
AU  - Martinović, Jelena
AU  - Guševac Stojanović, Ivana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11061
AB  - Adenosine 5'-triphosphate (ATP) and adenosine are versatile signaling molecules involved in many pathophysiological processes in the nervous system. They can be released from all types of brain cells in the extracellular space and activates purinergic receptors. Signaling via extracellular ATP is regulated by cell-surface located ectonucleotidases. Extracellular AMP resulting from the hydrolysis of ATP and ADP can in turn be hydrolyzed into adenosine by ecto-5'-nucleotidase (eN). We examined the involvement of purinergic signaling components in the rat model of trimethyltin (TMT)-induced hippocampal neurodegeneration (8mg/kg, single ip), which results in behavioral and neurological dysfunction similar as in Alzheimer's disease models. Enzyme histochemistry and immunohistochemistry (ir) showed that products of AMPase activity and eN-ir were accumulated in the neuronal strata, infiltrating within neuronal cell layers, depicting individual round-shaped elements that covered neuronal layers with pronounced cell death mostly at the late stage of TMT-induced neurodegeneration. Co-localization with Iba1+ specifically marked eN at amoeboid microglial cells. Neither of the tested pro-inflammatory cytokines (IL-1β, TNF-α, IL10) and C3 nor polarization marker iNOS was found in association with those Iba1/eN+ -cells. Iba1-ir cells co-localized with Arg1-ir and phagocytic marker CD68- ir. Marked induction of P2Y12R-, P2Y6R-, and P2X4-mRNA at the early stage of TMT-induced neurodegeneration might reflect the migration, and chemotaxis of microglia, while induction of P2X7R at amoeboid cells probably modulates their phagocytic role. These findings may contribute to a better understanding of the involvement of purinergic signaling components in the progression of neurodegenerative disorders that could be target molecules for development of novel therapies.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Ecto-5'-nucleotidase marks amoeboid microglial cells in the rat model of neurodegeneration
SP  - 120
ER  - 

@conference{
author = "Grković, Ivana and Dragić, Milorad and Mitrović, Nataša and Zarić Kontić, Marina and Martinović, Jelena and Guševac Stojanović, Ivana",
year = "2023",
abstract = "Adenosine 5'-triphosphate (ATP) and adenosine are versatile signaling molecules involved in many pathophysiological processes in the nervous system. They can be released from all types of brain cells in the extracellular space and activates purinergic receptors. Signaling via extracellular ATP is regulated by cell-surface located ectonucleotidases. Extracellular AMP resulting from the hydrolysis of ATP and ADP can in turn be hydrolyzed into adenosine by ecto-5'-nucleotidase (eN). We examined the involvement of purinergic signaling components in the rat model of trimethyltin (TMT)-induced hippocampal neurodegeneration (8mg/kg, single ip), which results in behavioral and neurological dysfunction similar as in Alzheimer's disease models. Enzyme histochemistry and immunohistochemistry (ir) showed that products of AMPase activity and eN-ir were accumulated in the neuronal strata, infiltrating within neuronal cell layers, depicting individual round-shaped elements that covered neuronal layers with pronounced cell death mostly at the late stage of TMT-induced neurodegeneration. Co-localization with Iba1+ specifically marked eN at amoeboid microglial cells. Neither of the tested pro-inflammatory cytokines (IL-1β, TNF-α, IL10) and C3 nor polarization marker iNOS was found in association with those Iba1/eN+ -cells. Iba1-ir cells co-localized with Arg1-ir and phagocytic marker CD68- ir. Marked induction of P2Y12R-, P2Y6R-, and P2X4-mRNA at the early stage of TMT-induced neurodegeneration might reflect the migration, and chemotaxis of microglia, while induction of P2X7R at amoeboid cells probably modulates their phagocytic role. These findings may contribute to a better understanding of the involvement of purinergic signaling components in the progression of neurodegenerative disorders that could be target molecules for development of novel therapies.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Ecto-5'-nucleotidase marks amoeboid microglial cells in the rat model of neurodegeneration",
pages = "120"
}

Grković, I., Dragić, M., Mitrović, N., Zarić Kontić, M., Martinović, J.,& Guševac Stojanović, I.. (2023). Ecto-5'-nucleotidase marks amoeboid microglial cells in the rat model of neurodegeneration. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 120.

Grković I, Dragić M, Mitrović N, Zarić Kontić M, Martinović J, Guševac Stojanović I. Ecto-5'-nucleotidase marks amoeboid microglial cells in the rat model of neurodegeneration. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:120..

Grković, Ivana, Dragić, Milorad, Mitrović, Nataša, Zarić Kontić, Marina, Martinović, Jelena, Guševac Stojanović, Ivana, "Ecto-5'-nucleotidase marks amoeboid microglial cells in the rat model of neurodegeneration" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):120.

TY  - CONF
AU  - Bobić, Katarina
AU  - Gušević Stojanović, Ivana
AU  - Todorović, Ana
AU  - Veljković, Filip
AU  - Pejić, Snežana
AU  - Martinović, Jelena
AU  - Drakulić, Dunja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11060
AB  - Prolonged disturbance of cerebral blood flow causes metabolic insufficiency and neuronal hypofunction, for which there is still no adequate therapeutic strategy. In several animal models of neurodegenerative diseases, progesterone (P4), a potent gonadal steroid hormone, and its metabolites showed neuroprotective outcomes through reduction of oxidative stress and stabilization of membrane lipids and their downstream signalling. As P4 actions in rat striatum following permanent bilateral occlusion of both common carotid arteries are still uncertain, we investigated its capacity to reduce neuronal damage induced by permanent occlusion of both carotid arteries (2VO), focusing on several oxidative stress markers (end products of lipid peroxidation (LPP) and phosphatidylcholine (PC) to lysophosphatidylcholine (LPC) intensity ratio) in crude synaptosomal fraction. Adult male Wistar rats were divided into groups: control ‒ sham operated animals treated with vehicle (commercial flax oil, 1 mg/kg) and permanently occluded animals subjected to either vehicle (commercial flax oil, 1 mg/kg) or P4 (dissolved in commercial flax oil, 1.7 mg/kg). Animals were subcutaneously injected for 7 days and sacrificed 4 h following the last treatment. LPP levels were determined spectrophotometrically, while PC/LPC intensity ratio was estimated by mass spectrometer. Obtained results indicate that P4 treatment alleviates 2VO – induced prooxidative changes by decreasing LPP levels and elevating PC/LPC intensity ratio, and returning them closer to levels observed in controls. According to our findings, P4 treatment in cerebral hypoperfusion model, via targeting striatal cell lipid components and altering lipid profile, might be implicated in reduction of oxidative stress and promotion of protective environment.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Progesterone modulates striatal lipid profile in rat cerebral hypoperfusion model
SP  - 118
ER  - 

@conference{
author = "Bobić, Katarina and Gušević Stojanović, Ivana and Todorović, Ana and Veljković, Filip and Pejić, Snežana and Martinović, Jelena and Drakulić, Dunja",
year = "2023",
abstract = "Prolonged disturbance of cerebral blood flow causes metabolic insufficiency and neuronal hypofunction, for which there is still no adequate therapeutic strategy. In several animal models of neurodegenerative diseases, progesterone (P4), a potent gonadal steroid hormone, and its metabolites showed neuroprotective outcomes through reduction of oxidative stress and stabilization of membrane lipids and their downstream signalling. As P4 actions in rat striatum following permanent bilateral occlusion of both common carotid arteries are still uncertain, we investigated its capacity to reduce neuronal damage induced by permanent occlusion of both carotid arteries (2VO), focusing on several oxidative stress markers (end products of lipid peroxidation (LPP) and phosphatidylcholine (PC) to lysophosphatidylcholine (LPC) intensity ratio) in crude synaptosomal fraction. Adult male Wistar rats were divided into groups: control ‒ sham operated animals treated with vehicle (commercial flax oil, 1 mg/kg) and permanently occluded animals subjected to either vehicle (commercial flax oil, 1 mg/kg) or P4 (dissolved in commercial flax oil, 1.7 mg/kg). Animals were subcutaneously injected for 7 days and sacrificed 4 h following the last treatment. LPP levels were determined spectrophotometrically, while PC/LPC intensity ratio was estimated by mass spectrometer. Obtained results indicate that P4 treatment alleviates 2VO – induced prooxidative changes by decreasing LPP levels and elevating PC/LPC intensity ratio, and returning them closer to levels observed in controls. According to our findings, P4 treatment in cerebral hypoperfusion model, via targeting striatal cell lipid components and altering lipid profile, might be implicated in reduction of oxidative stress and promotion of protective environment.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Progesterone modulates striatal lipid profile in rat cerebral hypoperfusion model",
pages = "118"
}

Bobić, K., Gušević Stojanović, I., Todorović, A., Veljković, F., Pejić, S., Martinović, J.,& Drakulić, D.. (2023). Progesterone modulates striatal lipid profile in rat cerebral hypoperfusion model. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 118.

Bobić K, Gušević Stojanović I, Todorović A, Veljković F, Pejić S, Martinović J, Drakulić D. Progesterone modulates striatal lipid profile in rat cerebral hypoperfusion model. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:118..

Bobić, Katarina, Gušević Stojanović, Ivana, Todorović, Ana, Veljković, Filip, Pejić, Snežana, Martinović, Jelena, Drakulić, Dunja, "Progesterone modulates striatal lipid profile in rat cerebral hypoperfusion model" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):118.

TY  - CONF
AU  - Mitić, Miloš
AU  - Lukić, Iva
AU  - Glavonić, Emilija
AU  - Živanović, Ana
AU  - Mijović, Milica
AU  - Ivković, Sanja
AU  - Adžić, Miroslav
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11054
AB  - Adolescence is a critical period for neurodevelopment, and exposure to chronic stress during this stage can have long-lasting effects on physiological systems and mental health, particularly on depression. Recent studies report that stress affects the gutbrain axis, leading to changes in gut morphology and motility, nutrient absorption, and gut microbiome, which can be associated with development of depression. We investigated the impact of chronic unpredictable stress (CUS) in adolescence on depressive-like behavior and colon in adult mice. Male C57BL/6 mice were exposed to CUS, including different daily stressors such as social isolation, forced swim, and restraint stress, and others, during postnatal days 28-40. Control mice were housed under standard conditions. Behavioral assessments were conducted during adulthood (postnatal day 70), to evaluate depressive-like behavior. Alterations in mice colon were assessed by histopathological analysis. Our results revealed that mice exposed to CUS during adolescence have disrupted colon, including loss of colonic crypts and significantly increased presence of mucosa and submucosa in respect to controls. Changes in colon were associated with increased depressive-like behavior in CUS-mice compared to control mice. These findings suggest that CUS experienced in adolescence can disrupt colon morphology that is associated with depressive phenotype in adult mice, highlighting the importance of understanding the long-term consequences of chronic stress during this critical period of development as a potential risk for development of depression. Further research is needed to elucidate the underlying mechanisms and potential therapeutic interventions to mitigate the effects of stress on mental health and gut function
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Chronic unpredictable stress in adolescence causes disruption of colon morphology that is associated with depressive phenotype in adult mice
SP  - 85
ER  - 

@conference{
author = "Mitić, Miloš and Lukić, Iva and Glavonić, Emilija and Živanović, Ana and Mijović, Milica and Ivković, Sanja and Adžić, Miroslav",
year = "2023",
abstract = "Adolescence is a critical period for neurodevelopment, and exposure to chronic stress during this stage can have long-lasting effects on physiological systems and mental health, particularly on depression. Recent studies report that stress affects the gutbrain axis, leading to changes in gut morphology and motility, nutrient absorption, and gut microbiome, which can be associated with development of depression. We investigated the impact of chronic unpredictable stress (CUS) in adolescence on depressive-like behavior and colon in adult mice. Male C57BL/6 mice were exposed to CUS, including different daily stressors such as social isolation, forced swim, and restraint stress, and others, during postnatal days 28-40. Control mice were housed under standard conditions. Behavioral assessments were conducted during adulthood (postnatal day 70), to evaluate depressive-like behavior. Alterations in mice colon were assessed by histopathological analysis. Our results revealed that mice exposed to CUS during adolescence have disrupted colon, including loss of colonic crypts and significantly increased presence of mucosa and submucosa in respect to controls. Changes in colon were associated with increased depressive-like behavior in CUS-mice compared to control mice. These findings suggest that CUS experienced in adolescence can disrupt colon morphology that is associated with depressive phenotype in adult mice, highlighting the importance of understanding the long-term consequences of chronic stress during this critical period of development as a potential risk for development of depression. Further research is needed to elucidate the underlying mechanisms and potential therapeutic interventions to mitigate the effects of stress on mental health and gut function",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Chronic unpredictable stress in adolescence causes disruption of colon morphology that is associated with depressive phenotype in adult mice",
pages = "85"
}

Mitić, M., Lukić, I., Glavonić, E., Živanović, A., Mijović, M., Ivković, S.,& Adžić, M.. (2023). Chronic unpredictable stress in adolescence causes disruption of colon morphology that is associated with depressive phenotype in adult mice. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 85.

Mitić M, Lukić I, Glavonić E, Živanović A, Mijović M, Ivković S, Adžić M. Chronic unpredictable stress in adolescence causes disruption of colon morphology that is associated with depressive phenotype in adult mice. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:85..

Mitić, Miloš, Lukić, Iva, Glavonić, Emilija, Živanović, Ana, Mijović, Milica, Ivković, Sanja, Adžić, Miroslav, "Chronic unpredictable stress in adolescence causes disruption of colon morphology that is associated with depressive phenotype in adult mice" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):85.

TY  - CONF
AU  - Virijević, Kristina
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Ferizović, Harisa
AU  - Janković, Milica
AU  - Dronjak, Slađana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11053
AB  - Stress is a major precipitant of depression, the hippocampus (HPC) and prefrontal cortex (PFC) is the main structures affected by depressive disorders. Women are twice more likely to experience depression than men. The WKY rat strain has long been established as a model of depression. Initially bred from the Wistar (WI) rat as the control strain for the spontaneously hypertensive rat, WKY rats demonstrate an exaggerated stress response compared to other strains. WKY strain fails to respond to chronic antidepressant treatment after exposure to chronic mild stress (CMS) and is considered to be nonresponsive to antidepressant drugs. MAPK signaling pathway was most closely related to depression and antidepressant treatment. In the present study, we have examined the effects of CMS on behavior and p38 MAPK signaling in the hippocampus and the medial prefrontal cortex (mPFC) of female WKY rats. We used two very different behavioral tests: forced swim test (FST) and open field test (OFT). WKY unstressed controls exhibited increased immobility duration in the forced swim test and decreased activity in the open-field test compared to unstressed WI rats, while CMS did not further influence behavior. WKY showed increased expression of the p38 only in HPC and further exposure of WKY rats to CMS induces sustained-activation of p38 MAPK in this brain area. The present study demonstrated the brain region-specific protein signatures in the female WKY model with endogenous depression, providing novel insights into the pathogenesis of depression.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Chronic mild stress induces sustained-activation of p38 MAPK signaling in the female WKY rats with endogenous depression
SP  - 84
ER  - 

@conference{
author = "Virijević, Kristina and Spasojević, Nataša and Stefanović, Bojana and Ferizović, Harisa and Janković, Milica and Dronjak, Slađana",
year = "2023",
abstract = "Stress is a major precipitant of depression, the hippocampus (HPC) and prefrontal cortex (PFC) is the main structures affected by depressive disorders. Women are twice more likely to experience depression than men. The WKY rat strain has long been established as a model of depression. Initially bred from the Wistar (WI) rat as the control strain for the spontaneously hypertensive rat, WKY rats demonstrate an exaggerated stress response compared to other strains. WKY strain fails to respond to chronic antidepressant treatment after exposure to chronic mild stress (CMS) and is considered to be nonresponsive to antidepressant drugs. MAPK signaling pathway was most closely related to depression and antidepressant treatment. In the present study, we have examined the effects of CMS on behavior and p38 MAPK signaling in the hippocampus and the medial prefrontal cortex (mPFC) of female WKY rats. We used two very different behavioral tests: forced swim test (FST) and open field test (OFT). WKY unstressed controls exhibited increased immobility duration in the forced swim test and decreased activity in the open-field test compared to unstressed WI rats, while CMS did not further influence behavior. WKY showed increased expression of the p38 only in HPC and further exposure of WKY rats to CMS induces sustained-activation of p38 MAPK in this brain area. The present study demonstrated the brain region-specific protein signatures in the female WKY model with endogenous depression, providing novel insights into the pathogenesis of depression.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Chronic mild stress induces sustained-activation of p38 MAPK signaling in the female WKY rats with endogenous depression",
pages = "84"
}

Virijević, K., Spasojević, N., Stefanović, B., Ferizović, H., Janković, M.,& Dronjak, S.. (2023). Chronic mild stress induces sustained-activation of p38 MAPK signaling in the female WKY rats with endogenous depression. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 84.

Virijević K, Spasojević N, Stefanović B, Ferizović H, Janković M, Dronjak S. Chronic mild stress induces sustained-activation of p38 MAPK signaling in the female WKY rats with endogenous depression. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:84..

Virijević, Kristina, Spasojević, Nataša, Stefanović, Bojana, Ferizović, Harisa, Janković, Milica, Dronjak, Slađana, "Chronic mild stress induces sustained-activation of p38 MAPK signaling in the female WKY rats with endogenous depression" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):84.

TY  - CONF
AU  - Janković, Milica
AU  - Ferizović, Harisa
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Virijević, Kristina
AU  - Dronjak, Slađana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11059
AB  - Modulating the endocannabinoid system is emerging as a promising approach for treating various inflammatory, neurodegenerative, and psychiatric disorders. Major depressive disorder is a prevalent cause of disability worldwide, affecting up to 6% of the population and posing significant health and economic challenges. Since the existing antidepressant treatments are often insufficient, the endocannabinoid system presents an attractive target for potential therapies. To explore this possibility, two-month-old Wistar rats of both sexes were subjected to chronic unpredictable stress (CUS) for six weeks. During the last two weeks of stress protocol rats were injected with either a fatty acid amide hydrolase inhibitor, URB597 (i.p. 0.3 mg/kg), or a vehicle. At the end of the sixth week sucrose intake test was used to assess depressive-like behavior. After sacrificing the animals, Western blot analysis was used to determine the levels of brain-derived neurotrophic factor (BDNF) protein and levels of pro-inflammatory cytokine IL-1β in medial prefrontal cortex. The CUS exposure induced anhedonia in both female and male rats, and URB597 treatment alleviated these symptoms. Moreover, CUS increased the levels of IL1-β in the mPFC of both sexes. Stressed animals that have received URB597 had decreased levels of IL1-β structures compared to the animals receiving only vehicle. CUSexposed male and female rats also had lower levels of BDNF in the mPFC, but URB597 restored BDNF levels in female rats to those observed in control animals. Taken together, these findings suggest that URB597 may have antidepressant effects through reducing neuroinflammation and restoring BDNF levels in mPFC.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Fatty acid amide hydrolase inhibitor URB597 shows antidepressant effects through reduction of neuroinflammation and restoration of BDNF levels in mPFC of chronically stressed rats
SP  - 118
ER  - 

@conference{
author = "Janković, Milica and Ferizović, Harisa and Spasojević, Nataša and Stefanović, Bojana and Virijević, Kristina and Dronjak, Slađana",
year = "2023",
abstract = "Modulating the endocannabinoid system is emerging as a promising approach for treating various inflammatory, neurodegenerative, and psychiatric disorders. Major depressive disorder is a prevalent cause of disability worldwide, affecting up to 6% of the population and posing significant health and economic challenges. Since the existing antidepressant treatments are often insufficient, the endocannabinoid system presents an attractive target for potential therapies. To explore this possibility, two-month-old Wistar rats of both sexes were subjected to chronic unpredictable stress (CUS) for six weeks. During the last two weeks of stress protocol rats were injected with either a fatty acid amide hydrolase inhibitor, URB597 (i.p. 0.3 mg/kg), or a vehicle. At the end of the sixth week sucrose intake test was used to assess depressive-like behavior. After sacrificing the animals, Western blot analysis was used to determine the levels of brain-derived neurotrophic factor (BDNF) protein and levels of pro-inflammatory cytokine IL-1β in medial prefrontal cortex. The CUS exposure induced anhedonia in both female and male rats, and URB597 treatment alleviated these symptoms. Moreover, CUS increased the levels of IL1-β in the mPFC of both sexes. Stressed animals that have received URB597 had decreased levels of IL1-β structures compared to the animals receiving only vehicle. CUSexposed male and female rats also had lower levels of BDNF in the mPFC, but URB597 restored BDNF levels in female rats to those observed in control animals. Taken together, these findings suggest that URB597 may have antidepressant effects through reducing neuroinflammation and restoring BDNF levels in mPFC.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Fatty acid amide hydrolase inhibitor URB597 shows antidepressant effects through reduction of neuroinflammation and restoration of BDNF levels in mPFC of chronically stressed rats",
pages = "118"
}

Janković, M., Ferizović, H., Spasojević, N., Stefanović, B., Virijević, K.,& Dronjak, S.. (2023). Fatty acid amide hydrolase inhibitor URB597 shows antidepressant effects through reduction of neuroinflammation and restoration of BDNF levels in mPFC of chronically stressed rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 118.

Janković M, Ferizović H, Spasojević N, Stefanović B, Virijević K, Dronjak S. Fatty acid amide hydrolase inhibitor URB597 shows antidepressant effects through reduction of neuroinflammation and restoration of BDNF levels in mPFC of chronically stressed rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:118..

Janković, Milica, Ferizović, Harisa, Spasojević, Nataša, Stefanović, Bojana, Virijević, Kristina, Dronjak, Slađana, "Fatty acid amide hydrolase inhibitor URB597 shows antidepressant effects through reduction of neuroinflammation and restoration of BDNF levels in mPFC of chronically stressed rats" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):118.

TY  - CONF
AU  - Guševac Stojanović, Ivana
AU  - Dragić, Milorad
AU  - Zarić Kontić, Marina
AU  - Martinović, J.
AU  - Mitrović, N.
AU  - Stojanović, Zoran
AU  - Veljković, Filip
AU  - Martinović, D.
AU  - Grković, Ivana
AU  - Drakulić, Dunja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11058
AB  - Cerebral hypoperfusion (CH) is recognised as a contributor to various impairments characteristic for elderly population and patients with vascular dementia and Alzheimer’s disease. CH-induced brain damage is linked with oxidative stress in the cells that can cause DNA fragmentation and cell death, reflected through a change in cells’ morphology. Our study investigated the beneficial effects of progesterone (P4), a hormone with neuroprotective properties, against CH-induced oxidative stress and neurodegenerative pathologies in rat prefrontal cortex (PFC). For the purpose of the experiment, adult male Wistar rats were dived into groups: (I) animals subjected to permanent bilateral occlusion of common carotid arteries (2VO) treated with vehicle (commercial flax oil, 1 mg/kg/day), (II) animals subjected to 2VO treated with P4 dissolved in vehicle (1.7 mg/kg/day) and (III) animals subjected to sham operation treated with vehicle. Animals were sacrificed after 7 subcutaneous treatments. Levels of pro-/antioxidant balance (PAB) and DNA fragmentation along with cell morphology were estimated by well-defined methods. The results revealed that P4 administration moderated CH-induced impairments in PFC, not only by decreasing PAB level and diminishing DNA fragmentation, but also preserving the cell morphology reflected through clearly defined cell bodies, with round nuclei, prominent nucleolus and visible Nissl bodies in layer III. Obtained results point out that P4 is able to attenuate CH-induced pro-oxidant state and subsequent changes in PFC. This hormone holds promise as an effective agent for the CH treatment, still, its specific actions remain to be discovered.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model
SP  - 117
ER  - 

@conference{
author = "Guševac Stojanović, Ivana and Dragić, Milorad and Zarić Kontić, Marina and Martinović, J. and Mitrović, N. and Stojanović, Zoran and Veljković, Filip and Martinović, D. and Grković, Ivana and Drakulić, Dunja",
year = "2023",
abstract = "Cerebral hypoperfusion (CH) is recognised as a contributor to various impairments characteristic for elderly population and patients with vascular dementia and Alzheimer’s disease. CH-induced brain damage is linked with oxidative stress in the cells that can cause DNA fragmentation and cell death, reflected through a change in cells’ morphology. Our study investigated the beneficial effects of progesterone (P4), a hormone with neuroprotective properties, against CH-induced oxidative stress and neurodegenerative pathologies in rat prefrontal cortex (PFC). For the purpose of the experiment, adult male Wistar rats were dived into groups: (I) animals subjected to permanent bilateral occlusion of common carotid arteries (2VO) treated with vehicle (commercial flax oil, 1 mg/kg/day), (II) animals subjected to 2VO treated with P4 dissolved in vehicle (1.7 mg/kg/day) and (III) animals subjected to sham operation treated with vehicle. Animals were sacrificed after 7 subcutaneous treatments. Levels of pro-/antioxidant balance (PAB) and DNA fragmentation along with cell morphology were estimated by well-defined methods. The results revealed that P4 administration moderated CH-induced impairments in PFC, not only by decreasing PAB level and diminishing DNA fragmentation, but also preserving the cell morphology reflected through clearly defined cell bodies, with round nuclei, prominent nucleolus and visible Nissl bodies in layer III. Obtained results point out that P4 is able to attenuate CH-induced pro-oxidant state and subsequent changes in PFC. This hormone holds promise as an effective agent for the CH treatment, still, its specific actions remain to be discovered.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model",
pages = "117"
}

Guševac Stojanović, I., Dragić, M., Zarić Kontić, M., Martinović, J., Mitrović, N., Stojanović, Z., Veljković, F., Martinović, D., Grković, I.,& Drakulić, D.. (2023). Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 117.

Guševac Stojanović I, Dragić M, Zarić Kontić M, Martinović J, Mitrović N, Stojanović Z, Veljković F, Martinović D, Grković I, Drakulić D. Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:117..

Guševac Stojanović, Ivana, Dragić, Milorad, Zarić Kontić, Marina, Martinović, J., Mitrović, N., Stojanović, Zoran, Veljković, Filip, Martinović, D., Grković, Ivana, Drakulić, Dunja, "Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):117.

TY  - CONF
AU  - Tasić, Jelena
AU  - Vidičević Novaković, Sašenka
AU  - Stanojević, Željka
AU  - Paunović, Verica
AU  - Petričević, Saša
AU  - Martinović, Tamara
AU  - Kravić-Stevović, Tamara
AU  - Ćirić, Darko
AU  - Marković, Zoran
AU  - Isaković, Aleksandra
AU  - Trajković, Vladimir
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11057
AB  - Background: Experimental autoimmune encephalomyelitis (EAE) is one of the most studied model of neuroinflammation, used to test immunomodulatory and antiinflammatory drugs. Graphene quantum dots (GQD) are oval graphite twodimensional sheets with a diameter <100 nm, one carbon atom thickness, with potential applications in biomedicine. Objective: To investigate the potential protective effect of GQD in EAE model. Methods: Female DA rats were immunized with spinal cord homogenate and Freund’s complete adjuvant. GQD treatment (10 mg/kg, ip) was administrated during the inductive, effector and both phases of a disease. MAP kinase (MAPK) and Akt activity in popliteal lymph nodes (PLN) and CNS was determined by western blot. Quantitative PCR and flow cytometry were used to examine the expression of proinflammatory cytokines and specific transcription factors while infiltration of GQD in cells/tissues was detected by transmission electron microscopy. GQD antiinflamatory/direct cytoprotective effect was analyzed on oligodendrocyte and neuron cell cultures by MTT assay. Data were analized by Mann Whitney test (p<0.05 was considered as statistical significant difference). Results: GQD administration, in all phases of EAE, significantly reduced clinical score of a disease. Clinical improvement correlates with increase in activity of ERK, p38 and Akt that is followed by reduction of Th1 cell response in PLN and infiltrated spinal coard T cells. Due to its capacity to infiltrate cells and tissues, GQD exhibits direct cytoprotective effect on CNS. Additionaly, GQD reduced the expression of proinflammatory cytokines in ConA stimulated lymphocytes. Conclusion: GQD alleviate EAE, through direct cytoprotective effect on CNS and inhibition of Th1 cell response.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Graphene Quantum Dots show protective effect in animal model of neuroinflammation
SP  - 114
ER  - 

@conference{
author = "Tasić, Jelena and Vidičević Novaković, Sašenka and Stanojević, Željka and Paunović, Verica and Petričević, Saša and Martinović, Tamara and Kravić-Stevović, Tamara and Ćirić, Darko and Marković, Zoran and Isaković, Aleksandra and Trajković, Vladimir",
year = "2023",
abstract = "Background: Experimental autoimmune encephalomyelitis (EAE) is one of the most studied model of neuroinflammation, used to test immunomodulatory and antiinflammatory drugs. Graphene quantum dots (GQD) are oval graphite twodimensional sheets with a diameter <100 nm, one carbon atom thickness, with potential applications in biomedicine. Objective: To investigate the potential protective effect of GQD in EAE model. Methods: Female DA rats were immunized with spinal cord homogenate and Freund’s complete adjuvant. GQD treatment (10 mg/kg, ip) was administrated during the inductive, effector and both phases of a disease. MAP kinase (MAPK) and Akt activity in popliteal lymph nodes (PLN) and CNS was determined by western blot. Quantitative PCR and flow cytometry were used to examine the expression of proinflammatory cytokines and specific transcription factors while infiltration of GQD in cells/tissues was detected by transmission electron microscopy. GQD antiinflamatory/direct cytoprotective effect was analyzed on oligodendrocyte and neuron cell cultures by MTT assay. Data were analized by Mann Whitney test (p<0.05 was considered as statistical significant difference). Results: GQD administration, in all phases of EAE, significantly reduced clinical score of a disease. Clinical improvement correlates with increase in activity of ERK, p38 and Akt that is followed by reduction of Th1 cell response in PLN and infiltrated spinal coard T cells. Due to its capacity to infiltrate cells and tissues, GQD exhibits direct cytoprotective effect on CNS. Additionaly, GQD reduced the expression of proinflammatory cytokines in ConA stimulated lymphocytes. Conclusion: GQD alleviate EAE, through direct cytoprotective effect on CNS and inhibition of Th1 cell response.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Graphene Quantum Dots show protective effect in animal model of neuroinflammation",
pages = "114"
}

Tasić, J., Vidičević Novaković, S., Stanojević, Ž., Paunović, V., Petričević, S., Martinović, T., Kravić-Stevović, T., Ćirić, D., Marković, Z., Isaković, A.,& Trajković, V.. (2023). Graphene Quantum Dots show protective effect in animal model of neuroinflammation. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 114.

Tasić J, Vidičević Novaković S, Stanojević Ž, Paunović V, Petričević S, Martinović T, Kravić-Stevović T, Ćirić D, Marković Z, Isaković A, Trajković V. Graphene Quantum Dots show protective effect in animal model of neuroinflammation. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:114..

Tasić, Jelena, Vidičević Novaković, Sašenka, Stanojević, Željka, Paunović, Verica, Petričević, Saša, Martinović, Tamara, Kravić-Stevović, Tamara, Ćirić, Darko, Marković, Zoran, Isaković, Aleksandra, Trajković, Vladimir, "Graphene Quantum Dots show protective effect in animal model of neuroinflammation" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):114.

TY  - CONF
AU  - Živanović, Ana
AU  - Mitrić, Miloš
AU  - Glavonić, Emilija
AU  - Lukić, Iva
AU  - Adžić, Miroslav
AU  - Ivković, Sanja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11052
AB  - Major depressive disorder (MDD) affects over 300 million people worldwide. The administration of the sub-anaesthetic dose of ketamine, an NMDAr antagonist, was recently approved as highly effective antidepressant whose therapeutic effects are associated with an increase in BDNF levels in the brain. However, lowering the effective dose of ketamine because of its adverse effects is an important goal. We assessed the changes in BDNF levels after the single administration of two subanesthetic doses of ketamine (6mg/kg, Ket6 and 10mg/kg, Ket10) in the chronic unpredictable stress (CUS) mouse model of depression-like behavior in different brain structures. Male C57BL/6J mice exposed to CUS were treated at the postnatal day 70 with either vehicle, Ket6, or Ket10. Following tail suspension test (TST), to assess depressive phenotype at 2- and 7-days post-treatment, animals were sacrificed and the prefrontal cortex (PFC), hippocampus, and striatum were isolated and processed for Western blot analyses. Statistical significance was determined by 1-way ANOVA. Only Ket6 achieved an antidepressant effect that was extinguished at 7 days. Both doses caused a significant increase in BDNF levels in the striatum while neither dose was able to induce BDNF levels in the hippocampus. The increase in BDNF levels in the PFC was observed only 7 days after the treatment and only with Ket10. The increase in BDNF levels was the greatest in the striatum when it correlated with the antidepressive effects of ketamine. Although this increase was sustained for 7 days it did not correlate with the antidepressive behavior which was already extinguished.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - The effect of different subanesthetic doses of ketamine on BDNF levels in different brain structures in the mouse model of depression
SP  - 83
ER  - 

@conference{
author = "Živanović, Ana and Mitrić, Miloš and Glavonić, Emilija and Lukić, Iva and Adžić, Miroslav and Ivković, Sanja",
year = "2023",
abstract = "Major depressive disorder (MDD) affects over 300 million people worldwide. The administration of the sub-anaesthetic dose of ketamine, an NMDAr antagonist, was recently approved as highly effective antidepressant whose therapeutic effects are associated with an increase in BDNF levels in the brain. However, lowering the effective dose of ketamine because of its adverse effects is an important goal. We assessed the changes in BDNF levels after the single administration of two subanesthetic doses of ketamine (6mg/kg, Ket6 and 10mg/kg, Ket10) in the chronic unpredictable stress (CUS) mouse model of depression-like behavior in different brain structures. Male C57BL/6J mice exposed to CUS were treated at the postnatal day 70 with either vehicle, Ket6, or Ket10. Following tail suspension test (TST), to assess depressive phenotype at 2- and 7-days post-treatment, animals were sacrificed and the prefrontal cortex (PFC), hippocampus, and striatum were isolated and processed for Western blot analyses. Statistical significance was determined by 1-way ANOVA. Only Ket6 achieved an antidepressant effect that was extinguished at 7 days. Both doses caused a significant increase in BDNF levels in the striatum while neither dose was able to induce BDNF levels in the hippocampus. The increase in BDNF levels in the PFC was observed only 7 days after the treatment and only with Ket10. The increase in BDNF levels was the greatest in the striatum when it correlated with the antidepressive effects of ketamine. Although this increase was sustained for 7 days it did not correlate with the antidepressive behavior which was already extinguished.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "The effect of different subanesthetic doses of ketamine on BDNF levels in different brain structures in the mouse model of depression",
pages = "83"
}

Živanović, A., Mitrić, M., Glavonić, E., Lukić, I., Adžić, M.,& Ivković, S.. (2023). The effect of different subanesthetic doses of ketamine on BDNF levels in different brain structures in the mouse model of depression. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 83.

Živanović A, Mitrić M, Glavonić E, Lukić I, Adžić M, Ivković S. The effect of different subanesthetic doses of ketamine on BDNF levels in different brain structures in the mouse model of depression. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:83..

Živanović, Ana, Mitrić, Miloš, Glavonić, Emilija, Lukić, Iva, Adžić, Miroslav, Ivković, Sanja, "The effect of different subanesthetic doses of ketamine on BDNF levels in different brain structures in the mouse model of depression" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):83.

TY  - CONF
AU  - Vasović, Dolika V.
AU  - Ivković, Sanja
AU  - Jovanović Macura, Irena
AU  - Major, Tamara
AU  - Živanović, Ana
AU  - Milašin, Jelena
AU  - Nikolić, Nađa
AU  - Simonović, Jelena
AU  - Šutulović, Nikola
AU  - Hrnčić, Dragan
AU  - Stanojlović, Olivera
AU  - Mladenović, Dušan
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11056
AB  - Introduction: Diabetic retinopathy (DR) is not cured efficiently and changes of lifestyle measures may alleviate its course. The aim of our study was to investigate the effects of shortened daily photoperiod on inflammation and visual cycle in rat retina and retinal pigment epithelium (RPE) in DR. Methodology: Male Wistar rats were divided into the following groups: 1. control; 2. diabetic group (DM) treated with a single intraperitoneal injection of streptozotocin (100 mg/kg); 3. group exposed to light/dark cycle 6/18h for 3 months (6/18); 4. diabetic group exposed to light/dark cycle 6/18h (DM+6/18). Retinal blood vessel permeability was estimated immunohistochemically based on lectin staining, while the expression of genes involved in the visual cycle (SOX9, LRAT, RPE65, OTX2), and inflammation (IL-1, TNF-α) was determined by qRT-PCR in the retina and RPE. Results: Shortened photoperiod reduced neovascularisation and the expression of IL-1 and TNF-α in both retina and RPE. The expression of IL-1 and TNF-α genes in the retina was significantly higher in DM vs. control group (P=0.001). In contrast, retinal IL-1 and TNF-α expressions were significantly lower in DM+6/18 vs. DM group (P=0.001). The expression of IL-1 and TNF-α in RPE was significantly higher in DM vs. control group, however the expression of these genes was significantly lower in DM+6/18 vs. DM group (PIL-1=0.008 and PTNF-α=0.002). The expression of visual cycle genes was significantly up-regulated in RPE in DM+6/18 vs. DM group (P=0.001). Conclusion: Shortened daily photoperiod reduces blood vessel permeability in DR via its anti-inflammatory effect associated with accelerated visual cycle in the retina.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - The effect of light/dark cycle changes on vascular permeability, inflammation and visual cycle in streptoyotocin-induced diabezic retinophaty in rats
SP  - 112
ER  - 

@conference{
author = "Vasović, Dolika V. and Ivković, Sanja and Jovanović Macura, Irena and Major, Tamara and Živanović, Ana and Milašin, Jelena and Nikolić, Nađa and Simonović, Jelena and Šutulović, Nikola and Hrnčić, Dragan and Stanojlović, Olivera and Mladenović, Dušan",
year = "2023",
abstract = "Introduction: Diabetic retinopathy (DR) is not cured efficiently and changes of lifestyle measures may alleviate its course. The aim of our study was to investigate the effects of shortened daily photoperiod on inflammation and visual cycle in rat retina and retinal pigment epithelium (RPE) in DR. Methodology: Male Wistar rats were divided into the following groups: 1. control; 2. diabetic group (DM) treated with a single intraperitoneal injection of streptozotocin (100 mg/kg); 3. group exposed to light/dark cycle 6/18h for 3 months (6/18); 4. diabetic group exposed to light/dark cycle 6/18h (DM+6/18). Retinal blood vessel permeability was estimated immunohistochemically based on lectin staining, while the expression of genes involved in the visual cycle (SOX9, LRAT, RPE65, OTX2), and inflammation (IL-1, TNF-α) was determined by qRT-PCR in the retina and RPE. Results: Shortened photoperiod reduced neovascularisation and the expression of IL-1 and TNF-α in both retina and RPE. The expression of IL-1 and TNF-α genes in the retina was significantly higher in DM vs. control group (P=0.001). In contrast, retinal IL-1 and TNF-α expressions were significantly lower in DM+6/18 vs. DM group (P=0.001). The expression of IL-1 and TNF-α in RPE was significantly higher in DM vs. control group, however the expression of these genes was significantly lower in DM+6/18 vs. DM group (PIL-1=0.008 and PTNF-α=0.002). The expression of visual cycle genes was significantly up-regulated in RPE in DM+6/18 vs. DM group (P=0.001). Conclusion: Shortened daily photoperiod reduces blood vessel permeability in DR via its anti-inflammatory effect associated with accelerated visual cycle in the retina.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "The effect of light/dark cycle changes on vascular permeability, inflammation and visual cycle in streptoyotocin-induced diabezic retinophaty in rats",
pages = "112"
}

Vasović, D. V., Ivković, S., Jovanović Macura, I., Major, T., Živanović, A., Milašin, J., Nikolić, N., Simonović, J., Šutulović, N., Hrnčić, D., Stanojlović, O.,& Mladenović, D.. (2023). The effect of light/dark cycle changes on vascular permeability, inflammation and visual cycle in streptoyotocin-induced diabezic retinophaty in rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 112.

Vasović DV, Ivković S, Jovanović Macura I, Major T, Živanović A, Milašin J, Nikolić N, Simonović J, Šutulović N, Hrnčić D, Stanojlović O, Mladenović D. The effect of light/dark cycle changes on vascular permeability, inflammation and visual cycle in streptoyotocin-induced diabezic retinophaty in rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:112..

Vasović, Dolika V., Ivković, Sanja, Jovanović Macura, Irena, Major, Tamara, Živanović, Ana, Milašin, Jelena, Nikolić, Nađa, Simonović, Jelena, Šutulović, Nikola, Hrnčić, Dragan, Stanojlović, Olivera, Mladenović, Dušan, "The effect of light/dark cycle changes on vascular permeability, inflammation and visual cycle in streptoyotocin-induced diabezic retinophaty in rats" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):112.

TY  - CONF
AU  - Glavonić, Emilija
AU  - Aleksić, Minja
AU  - Francija, Ester
AU  - Mitrić, Miloš
AU  - Lukić, Iva
AU  - Ivković, Sanja
AU  - Adžić, Miroslav
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11051
AB  - Adolescence is a developmental stage characterized by impaired fear extinction learning, which is a significant contributing factor for the high incidence of fearrelated disorders observed across this period. Ketamine is a noncompetitive N-methyl D-aspartate receptor antagonist that targets glutamatergic transmission and mammalian target of rapamycin (mTOR) signaling pathway, synaptic plasticity mediators known to be involved in fear extinction processes. Therefore, we aimed to explore ketamine’s potential to boost fear extinction of adolescent males, as well as to identify the associated molecular mechanisms. Adolescent male mice (C57BL/6) received an i.p. ketamine injection (10 mg/kg) 1h prior to each cued fear extinction session for 4 consecutive days. Protein expression levels of synaptic plasticity markers in hippocampal synaptosomal fractions were subsequently detected by Western blot analysis. Our results revealed that ketamine significantly improved overall fear extinction learning, as well as extinction memory consolidation/retention. Our data also showed that ketamine upregulated protein kinase B (Akt), mTOR and glutamate receptor 1 (GluR1) protein levels in the hippocampus. Interestingly, we detected no changes in the levels of extracellular signal-regulated kinase 1/2. These results suggest that ketamine ameliorates longterm fear extinction of adolescent males via hippocampal Akt-mTOR-GluR1 signaling, highlighting this pathway as an important therapeutic target for improving extinction learning in the adolescent population.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Ketamine ameliorates fear extinction learning in adolescent males via hippocampal mTOR signaling
SP  - 82
ER  - 

@conference{
author = "Glavonić, Emilija and Aleksić, Minja and Francija, Ester and Mitrić, Miloš and Lukić, Iva and Ivković, Sanja and Adžić, Miroslav",
year = "2023",
abstract = "Adolescence is a developmental stage characterized by impaired fear extinction learning, which is a significant contributing factor for the high incidence of fearrelated disorders observed across this period. Ketamine is a noncompetitive N-methyl D-aspartate receptor antagonist that targets glutamatergic transmission and mammalian target of rapamycin (mTOR) signaling pathway, synaptic plasticity mediators known to be involved in fear extinction processes. Therefore, we aimed to explore ketamine’s potential to boost fear extinction of adolescent males, as well as to identify the associated molecular mechanisms. Adolescent male mice (C57BL/6) received an i.p. ketamine injection (10 mg/kg) 1h prior to each cued fear extinction session for 4 consecutive days. Protein expression levels of synaptic plasticity markers in hippocampal synaptosomal fractions were subsequently detected by Western blot analysis. Our results revealed that ketamine significantly improved overall fear extinction learning, as well as extinction memory consolidation/retention. Our data also showed that ketamine upregulated protein kinase B (Akt), mTOR and glutamate receptor 1 (GluR1) protein levels in the hippocampus. Interestingly, we detected no changes in the levels of extracellular signal-regulated kinase 1/2. These results suggest that ketamine ameliorates longterm fear extinction of adolescent males via hippocampal Akt-mTOR-GluR1 signaling, highlighting this pathway as an important therapeutic target for improving extinction learning in the adolescent population.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Ketamine ameliorates fear extinction learning in adolescent males via hippocampal mTOR signaling",
pages = "82"
}

Glavonić, E., Aleksić, M., Francija, E., Mitrić, M., Lukić, I., Ivković, S.,& Adžić, M.. (2023). Ketamine ameliorates fear extinction learning in adolescent males via hippocampal mTOR signaling. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 82.

Glavonić E, Aleksić M, Francija E, Mitrić M, Lukić I, Ivković S, Adžić M. Ketamine ameliorates fear extinction learning in adolescent males via hippocampal mTOR signaling. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:82..

Glavonić, Emilija, Aleksić, Minja, Francija, Ester, Mitrić, Miloš, Lukić, Iva, Ivković, Sanja, Adžić, Miroslav, "Ketamine ameliorates fear extinction learning in adolescent males via hippocampal mTOR signaling" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):82.

TY  - CONF
AU  - Adžić Bukvić, Marija
AU  - Dragić, Milorad
AU  - Zarić Kontić, Marina
AU  - Nedeljković, Nadežda
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11055
AB  - The antibiotic streptozotocin (STZ) is an FDA approved for pancreatic neuroendocrine tumors. It has also been used for rat model of diabetes induction, where it causes a progressive increase in BBB permeability, and activates glial cells. In intracerebroventricular injected STZ-induced AD model, the abnormal mitochondrial morphology, decrease ATP biosynthesis, accumulation of reactive oxygen species (ROS), disrupted homeostasis of brain insulin signaling and defect in cerebral glucose metabolism were observed. Streptozotocin has been used to induce mitochondrial, endoplasmic and in general oxidative stress in neuronal cells and in astrocytoma C6 cell line in vitro. Our study aimed to analyze the STZ effects on primary rat astrocyte cultures. The testing of STZ concentration range (1, 5, 10 and 20 mM) in MTT assay, excluded the 20 mM STZ which evoked a significant decrease in mitochondrial activity in astrocytes. As ROS are the most pronounced parameters elevated in STZ disease modeling, we analyzed GSH, SH groups and MDA 24 h after the STZ application. The 10 mM STZ lowered GSH levels, while SH groups showed a STZ dose dependent decrease. On the other hand, MDA showed a slight, but not significant increase following STZ concentration increase. Moreover, we investigated changes in the purinergic signaling system. Our results show the drop of CD73 activity 24 h after the 10 mM STZ treatment, accompanied by CD73 immunofluorescence decrease on the astrocyte membranes. Similarly, nucleoside triphosphate diphosphohydrolase 2 (NT2) was downregulated on astrocyte membranes. These results encourage further analysis of the P1 and P2 purinergic receptors
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Streptozotocin, an FDA approved drug, affects the oxidative stress parameters and purinergic signaling components in primary rat astrocyte cultures
SP  - 102
ER  - 

@conference{
author = "Adžić Bukvić, Marija and Dragić, Milorad and Zarić Kontić, Marina and Nedeljković, Nadežda",
year = "2023",
abstract = "The antibiotic streptozotocin (STZ) is an FDA approved for pancreatic neuroendocrine tumors. It has also been used for rat model of diabetes induction, where it causes a progressive increase in BBB permeability, and activates glial cells. In intracerebroventricular injected STZ-induced AD model, the abnormal mitochondrial morphology, decrease ATP biosynthesis, accumulation of reactive oxygen species (ROS), disrupted homeostasis of brain insulin signaling and defect in cerebral glucose metabolism were observed. Streptozotocin has been used to induce mitochondrial, endoplasmic and in general oxidative stress in neuronal cells and in astrocytoma C6 cell line in vitro. Our study aimed to analyze the STZ effects on primary rat astrocyte cultures. The testing of STZ concentration range (1, 5, 10 and 20 mM) in MTT assay, excluded the 20 mM STZ which evoked a significant decrease in mitochondrial activity in astrocytes. As ROS are the most pronounced parameters elevated in STZ disease modeling, we analyzed GSH, SH groups and MDA 24 h after the STZ application. The 10 mM STZ lowered GSH levels, while SH groups showed a STZ dose dependent decrease. On the other hand, MDA showed a slight, but not significant increase following STZ concentration increase. Moreover, we investigated changes in the purinergic signaling system. Our results show the drop of CD73 activity 24 h after the 10 mM STZ treatment, accompanied by CD73 immunofluorescence decrease on the astrocyte membranes. Similarly, nucleoside triphosphate diphosphohydrolase 2 (NT2) was downregulated on astrocyte membranes. These results encourage further analysis of the P1 and P2 purinergic receptors",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Streptozotocin, an FDA approved drug, affects the oxidative stress parameters and purinergic signaling components in primary rat astrocyte cultures",
pages = "102"
}

Adžić Bukvić, M., Dragić, M., Zarić Kontić, M.,& Nedeljković, N.. (2023). Streptozotocin, an FDA approved drug, affects the oxidative stress parameters and purinergic signaling components in primary rat astrocyte cultures. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 102.

Adžić Bukvić M, Dragić M, Zarić Kontić M, Nedeljković N. Streptozotocin, an FDA approved drug, affects the oxidative stress parameters and purinergic signaling components in primary rat astrocyte cultures. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:102..

Adžić Bukvić, Marija, Dragić, Milorad, Zarić Kontić, Marina, Nedeljković, Nadežda, "Streptozotocin, an FDA approved drug, affects the oxidative stress parameters and purinergic signaling components in primary rat astrocyte cultures" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):102.

TY  - JOUR
AU  - Martinović, Jelena
AU  - Zarić Kontić, Marina
AU  - Dragić, Milorad
AU  - Todorović, Ana
AU  - Guševac Stojanović, Ivana
AU  - Mitrović, Nataša
AU  - Grković, Ivana
AU  - Drakulić, Dunja R.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10461
AB  - D-galactose (d-gal) is broadly used in animal aging studies as its chronic administration mimics learning and memory impairments related to aging in humans. However, within the few studies that utilize chronic oral d-gal intake, none of them is focused on alteration in synaptic structure and function. We examined the effects of 6-weeks oral d-gal intake (200 mg/kg and 500 mg/kg, dissolved in tap water) on age-related changes, with emphasis on the prefrontal cortex (PFC) and hippocampus (HIP) of adult male Wistar rats. Memory assessment was followed by histological examination of the PFC and HIP (Nissl staining and Iba-1 immunostaining), while in crude synaptosomal fractions the state of oxidative stress and the expression of proteins involved in glutamatergic signaling was determined. Although applied dosages compromised memory, alterations such as impaired sensory-motor function and aberrant morphology were not detected. In the PFC, analysis of microglia revealed reduction of branching pattern following d-gal intake, in parallel with increased oxidative damage of proteins, lipids and disturbed pro-oxidant antioxidant balance. These changes in the PFC were further accompanied with decreased levels of vesicular glutamate transporter 1, syntaxin-1 and NMDA receptor 2B subunit in both treated groups. Simultaneously, the increased hippocampal oxidative damage of lipids was detected. Results indicate successful provocation of age-related changes following oral d-gal intake, and suggest greater sensitivity of the PFC to d-gal treatment than HIP.
T2  - Behavioural Brain Research
T1  - Chronic oral d-galactose intake provokes age-related changes in the rat prefrontal cortex
VL  - 436
SP  - 114072
DO  - 10.1016/j.bbr.2022.114072
ER  - 

@article{
author = "Martinović, Jelena and Zarić Kontić, Marina and Dragić, Milorad and Todorović, Ana and Guševac Stojanović, Ivana and Mitrović, Nataša and Grković, Ivana and Drakulić, Dunja R.",
year = "2023",
abstract = "D-galactose (d-gal) is broadly used in animal aging studies as its chronic administration mimics learning and memory impairments related to aging in humans. However, within the few studies that utilize chronic oral d-gal intake, none of them is focused on alteration in synaptic structure and function. We examined the effects of 6-weeks oral d-gal intake (200 mg/kg and 500 mg/kg, dissolved in tap water) on age-related changes, with emphasis on the prefrontal cortex (PFC) and hippocampus (HIP) of adult male Wistar rats. Memory assessment was followed by histological examination of the PFC and HIP (Nissl staining and Iba-1 immunostaining), while in crude synaptosomal fractions the state of oxidative stress and the expression of proteins involved in glutamatergic signaling was determined. Although applied dosages compromised memory, alterations such as impaired sensory-motor function and aberrant morphology were not detected. In the PFC, analysis of microglia revealed reduction of branching pattern following d-gal intake, in parallel with increased oxidative damage of proteins, lipids and disturbed pro-oxidant antioxidant balance. These changes in the PFC were further accompanied with decreased levels of vesicular glutamate transporter 1, syntaxin-1 and NMDA receptor 2B subunit in both treated groups. Simultaneously, the increased hippocampal oxidative damage of lipids was detected. Results indicate successful provocation of age-related changes following oral d-gal intake, and suggest greater sensitivity of the PFC to d-gal treatment than HIP.",
journal = "Behavioural Brain Research",
title = "Chronic oral d-galactose intake provokes age-related changes in the rat prefrontal cortex",
volume = "436",
pages = "114072",
doi = "10.1016/j.bbr.2022.114072"
}

Martinović, J., Zarić Kontić, M., Dragić, M., Todorović, A., Guševac Stojanović, I., Mitrović, N., Grković, I.,& Drakulić, D. R.. (2023). Chronic oral d-galactose intake provokes age-related changes in the rat prefrontal cortex. in Behavioural Brain Research, 436, 114072.
https://doi.org/10.1016/j.bbr.2022.114072

Martinović J, Zarić Kontić M, Dragić M, Todorović A, Guševac Stojanović I, Mitrović N, Grković I, Drakulić DR. Chronic oral d-galactose intake provokes age-related changes in the rat prefrontal cortex. in Behavioural Brain Research. 2023;436:114072.
doi:10.1016/j.bbr.2022.114072 .

Martinović, Jelena, Zarić Kontić, Marina, Dragić, Milorad, Todorović, Ana, Guševac Stojanović, Ivana, Mitrović, Nataša, Grković, Ivana, Drakulić, Dunja R., "Chronic oral d-galactose intake provokes age-related changes in the rat prefrontal cortex" in Behavioural Brain Research, 436 (2023):114072,
https://doi.org/10.1016/j.bbr.2022.114072 . .

TY  - JOUR
AU  - Veljković, Filip M.
AU  - Dimitrijević, Stevan P.
AU  - Dimitrijević, Silvana B.
AU  - Vurdelja, Borislava D.
AU  - Matović, Branko
AU  - Stoiljković, Milovan
AU  - Kamberović, Željko
AU  - Veličković, Suzana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10589
AB  - This work presents the perspective of applying the laser desorption/ionization mass spectrometry (LDI MS) for characterization the anode film of the Ag60Cu26Zn14, Ag58.5Cu31.5Pd10, and Ag63Cu27In10 alloys (at high concentrations of chloride ions in solutions). The reference LDI mass spectra of anode films of pure Ag and Cu have been used for the identification of product corrosion. Knowing the clusters detected in the reference spectra lead to the facilitating identification of the LDI mass spectrum of the sample and reduces the analysis time. The LDI MS analysis of these alloys revealed that the predominant corrosion product are AgCl (from AgnCln+1−/+, n = 1–3), and CuCl (from “superhalogen” CumCln− clusters, m = 1–2, n = 2–6); it also revealed Cu2(OH)3Cl (from Cu2(OH)(H2O)2+) and Cu2O (from Cu(H2O)+, Cu2O doped with chlorine). These results are in accordance with the X-ray diffraction and Raman analysis. The LDI MS spectra of alloys contain the additional peaks formed due to the mutual influences of different metals in the alloys (AgCuCl3− (AgCl-CuCl2−), AgCu2Cl4− (AgCl-CuCl-CuCl2−), and Ag2CuCl4− (AgCl-AgCl-CuCl−), which is consistent with the identified corrosion products. It should be noted that the LDI MS suggest the presence of CuCl2, which can be interpreted as the corrosion products retained in the porous films of alloys, and not detected by the other methods due to a small amount. The future theoretical and experimental studies of metal clusters, significant for metallurgy, can contribute that the LDI MS is becoming a powerful analytical tool for characterization the metal surfaces.
T2  - Arabian Journal of Chemistry
T1  - Prospective of the LDI MS to characterization the corrosion products of silver-copper alloys on an example of the Ag-Cu-X (X- Zn, Pd, In) system
VL  - 16
IS  - 2
SP  - 104461
DO  - 10.1016/j.arabjc.2022.104461
ER  - 

@article{
author = "Veljković, Filip M. and Dimitrijević, Stevan P. and Dimitrijević, Silvana B. and Vurdelja, Borislava D. and Matović, Branko and Stoiljković, Milovan and Kamberović, Željko and Veličković, Suzana",
year = "2023",
abstract = "This work presents the perspective of applying the laser desorption/ionization mass spectrometry (LDI MS) for characterization the anode film of the Ag60Cu26Zn14, Ag58.5Cu31.5Pd10, and Ag63Cu27In10 alloys (at high concentrations of chloride ions in solutions). The reference LDI mass spectra of anode films of pure Ag and Cu have been used for the identification of product corrosion. Knowing the clusters detected in the reference spectra lead to the facilitating identification of the LDI mass spectrum of the sample and reduces the analysis time. The LDI MS analysis of these alloys revealed that the predominant corrosion product are AgCl (from AgnCln+1−/+, n = 1–3), and CuCl (from “superhalogen” CumCln− clusters, m = 1–2, n = 2–6); it also revealed Cu2(OH)3Cl (from Cu2(OH)(H2O)2+) and Cu2O (from Cu(H2O)+, Cu2O doped with chlorine). These results are in accordance with the X-ray diffraction and Raman analysis. The LDI MS spectra of alloys contain the additional peaks formed due to the mutual influences of different metals in the alloys (AgCuCl3− (AgCl-CuCl2−), AgCu2Cl4− (AgCl-CuCl-CuCl2−), and Ag2CuCl4− (AgCl-AgCl-CuCl−), which is consistent with the identified corrosion products. It should be noted that the LDI MS suggest the presence of CuCl2, which can be interpreted as the corrosion products retained in the porous films of alloys, and not detected by the other methods due to a small amount. The future theoretical and experimental studies of metal clusters, significant for metallurgy, can contribute that the LDI MS is becoming a powerful analytical tool for characterization the metal surfaces.",
journal = "Arabian Journal of Chemistry",
title = "Prospective of the LDI MS to characterization the corrosion products of silver-copper alloys on an example of the Ag-Cu-X (X- Zn, Pd, In) system",
volume = "16",
number = "2",
pages = "104461",
doi = "10.1016/j.arabjc.2022.104461"
}

Veljković, F. M., Dimitrijević, S. P., Dimitrijević, S. B., Vurdelja, B. D., Matović, B., Stoiljković, M., Kamberović, Ž.,& Veličković, S.. (2023). Prospective of the LDI MS to characterization the corrosion products of silver-copper alloys on an example of the Ag-Cu-X (X- Zn, Pd, In) system. in Arabian Journal of Chemistry, 16(2), 104461.
https://doi.org/10.1016/j.arabjc.2022.104461

Veljković FM, Dimitrijević SP, Dimitrijević SB, Vurdelja BD, Matović B, Stoiljković M, Kamberović Ž, Veličković S. Prospective of the LDI MS to characterization the corrosion products of silver-copper alloys on an example of the Ag-Cu-X (X- Zn, Pd, In) system. in Arabian Journal of Chemistry. 2023;16(2):104461.
doi:10.1016/j.arabjc.2022.104461 .

Veljković, Filip M., Dimitrijević, Stevan P., Dimitrijević, Silvana B., Vurdelja, Borislava D., Matović, Branko, Stoiljković, Milovan, Kamberović, Željko, Veličković, Suzana, "Prospective of the LDI MS to characterization the corrosion products of silver-copper alloys on an example of the Ag-Cu-X (X- Zn, Pd, In) system" in Arabian Journal of Chemistry, 16, no. 2 (2023):104461,
https://doi.org/10.1016/j.arabjc.2022.104461 . .

TY  - JOUR
AU  - Jelić, Dijana
AU  - Đermanović, Mirjana
AU  - Marković, Anđela
AU  - Manić, Nebojša
AU  - Veličković, Suzana
AU  - Veljković, Filip M.
AU  - Janković, Bojan Ž.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10738
AB  - This work provides detailed mechanistic analysis of thermo-oxidative degradation behavior of vitamin D3—Ca (CaCO3 form) solid state supplement formulation. Analytical techniques such Attenuated Total Reflection Fourier-transform Infrared (ATR-FTIR) spectroscopy and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) were used for analysis of homogeneity of active pharmaceutical ingredients (APIs) throughout excipients contained within solid dosage forms. Information gained from MALDI-MS experiments was used to improve and better understand interactions present, later clearly disclosed through kinetic modeling. It was found that process mechanism proceeds via two single-step unbranched reactions and two steps of consecutive reactions. Key features include vitamin D3 meltings and degradation via hydrogen abstraction followed by addition of oxygen forming 1-hydroxy-vitamin D3 and further through its dehydration to 1-keto-vitamin D3. Identified product 1-hydroxy-vitamin D3 is substantial for enhancing the immune response of human body in fight against respiratory viruses. Another two degradation products, namely pyrocalciferol and isopyrocalciferol, produced by thermal isomerization at higher temperatures, were also identified. These vitamin epimers have crucial role in functioning of immune cells. Degradation process of mineral structure occurs through water molecules removal, forming anhydrous polymorph of CaCO3, where formation of solid (CaO) and gaseous (CO2) products participates in kinetically stabilized additive-functionalized amorphous CaCO3 crystallization. Confirmation of correctness of proposed degradation mechanism was verified by modulated dynamic (MD) predictions. Information obtained is valuable and suitable for safety evaluations of given supplement, but also could be applied for solid state forms, which are generally sensitive to oxidative conditions. © 2023, Akadémiai Kiadó, Budapest, Hungary.
T2  - Journal of Thermal Analysis and Calorimetry
T1  - Novel insight in thermo-oxidative kinetics of vitamin D-based supplement formulation using TG–DTG–DTA, ATR-FTIR and MALDI-MS techniques
IS  - InPress
DO  - 10.1007/s10973-023-12017-3
ER  - 

@article{
author = "Jelić, Dijana and Đermanović, Mirjana and Marković, Anđela and Manić, Nebojša and Veličković, Suzana and Veljković, Filip M. and Janković, Bojan Ž.",
year = "2023",
abstract = "This work provides detailed mechanistic analysis of thermo-oxidative degradation behavior of vitamin D3—Ca (CaCO3 form) solid state supplement formulation. Analytical techniques such Attenuated Total Reflection Fourier-transform Infrared (ATR-FTIR) spectroscopy and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) were used for analysis of homogeneity of active pharmaceutical ingredients (APIs) throughout excipients contained within solid dosage forms. Information gained from MALDI-MS experiments was used to improve and better understand interactions present, later clearly disclosed through kinetic modeling. It was found that process mechanism proceeds via two single-step unbranched reactions and two steps of consecutive reactions. Key features include vitamin D3 meltings and degradation via hydrogen abstraction followed by addition of oxygen forming 1-hydroxy-vitamin D3 and further through its dehydration to 1-keto-vitamin D3. Identified product 1-hydroxy-vitamin D3 is substantial for enhancing the immune response of human body in fight against respiratory viruses. Another two degradation products, namely pyrocalciferol and isopyrocalciferol, produced by thermal isomerization at higher temperatures, were also identified. These vitamin epimers have crucial role in functioning of immune cells. Degradation process of mineral structure occurs through water molecules removal, forming anhydrous polymorph of CaCO3, where formation of solid (CaO) and gaseous (CO2) products participates in kinetically stabilized additive-functionalized amorphous CaCO3 crystallization. Confirmation of correctness of proposed degradation mechanism was verified by modulated dynamic (MD) predictions. Information obtained is valuable and suitable for safety evaluations of given supplement, but also could be applied for solid state forms, which are generally sensitive to oxidative conditions. © 2023, Akadémiai Kiadó, Budapest, Hungary.",
journal = "Journal of Thermal Analysis and Calorimetry",
title = "Novel insight in thermo-oxidative kinetics of vitamin D-based supplement formulation using TG–DTG–DTA, ATR-FTIR and MALDI-MS techniques",
number = "InPress",
doi = "10.1007/s10973-023-12017-3"
}

Jelić, D., Đermanović, M., Marković, A., Manić, N., Veličković, S., Veljković, F. M.,& Janković, B. Ž.. (2023). Novel insight in thermo-oxidative kinetics of vitamin D-based supplement formulation using TG–DTG–DTA, ATR-FTIR and MALDI-MS techniques. in Journal of Thermal Analysis and Calorimetry(InPress).
https://doi.org/10.1007/s10973-023-12017-3

Jelić D, Đermanović M, Marković A, Manić N, Veličković S, Veljković FM, Janković BŽ. Novel insight in thermo-oxidative kinetics of vitamin D-based supplement formulation using TG–DTG–DTA, ATR-FTIR and MALDI-MS techniques. in Journal of Thermal Analysis and Calorimetry. 2023;(InPress).
doi:10.1007/s10973-023-12017-3 .

Jelić, Dijana, Đermanović, Mirjana, Marković, Anđela, Manić, Nebojša, Veličković, Suzana, Veljković, Filip M., Janković, Bojan Ž., "Novel insight in thermo-oxidative kinetics of vitamin D-based supplement formulation using TG–DTG–DTA, ATR-FTIR and MALDI-MS techniques" in Journal of Thermal Analysis and Calorimetry, no. InPress (2023),
https://doi.org/10.1007/s10973-023-12017-3 . .

TY  - JOUR
AU  - Janković, Bojan
AU  - Papović, Snežana
AU  - Vraneš, Milan
AU  - Knežević, Teodora
AU  - Pržulj, Sanja
AU  - Zeljković, Saša
AU  - Veličković, Suzana
AU  - Veljković, Filip
AU  - Jelić, Dijana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10850
AB  - A powdered PVA/CaCO3 nanocomposite carrier was successfully fabricated for effective loading of small bioactive molecules, such as vitamin D3 (VD3). Generation of, namely, VD3/PVA/CaCO3 nanocomposite scaffold was carried out through an adsorption mechanism as a loading route of an active compound onto a powder carrier. Developed composites were characterized by structural, morphological and thermal analyses techniques, such as X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, MALDI (matrix-assisted laser desorption/ionization) – mass spectrometry (MS), Brunauer–Emmett–Teller (BET) – NLDFT (Non-local Density Functional Theory) method, scanning electron microscopy (SEM), simultaneous TG-DTG and coupled TG-MS. XRD results showed that the average crystallite size of synthesized VD3/PVA/CaCO3 amounts 32.93 nm exhibiting microstrain presence, where PVA incorporation causes non-uniform calcite lattice distortion. SEM analysis showed that VD3/PVA/CaCO3 nanocomposite scaffold contains agglomerated rhomboidal calcite particles with VD3 particles of irregular shapes attached. Fabricated VD3/PVA/CaCO3 clearly showed the existence of calcite “staircase” dendrites as the aftermath of inhibiting the effect of impurities on the growth of crystals in normal directions. It was determined that the decomposition of PVA additionally enhances the thermal stability of VD3, through the stabilization effect by acting on van der Waal’s forces during polyene formation, confirmed by MALDI-TOF MS results.
T2  - Journal of Materials Science
T1  - Biomineral nanocomposite scaffold (CaCO3/PVA based) carrier for improved stability of vitamin D3: characterization analysis and material properties
VL  - 58
IS  - 15
SP  - 6580
EP  - 6601
DO  - 10.1007/s10853-023-08453-z
ER  - 

@article{
author = "Janković, Bojan and Papović, Snežana and Vraneš, Milan and Knežević, Teodora and Pržulj, Sanja and Zeljković, Saša and Veličković, Suzana and Veljković, Filip and Jelić, Dijana",
year = "2023",
abstract = "A powdered PVA/CaCO3 nanocomposite carrier was successfully fabricated for effective loading of small bioactive molecules, such as vitamin D3 (VD3). Generation of, namely, VD3/PVA/CaCO3 nanocomposite scaffold was carried out through an adsorption mechanism as a loading route of an active compound onto a powder carrier. Developed composites were characterized by structural, morphological and thermal analyses techniques, such as X-ray powder diffraction (XRPD), Fourier-transform infrared (FTIR) spectroscopy, MALDI (matrix-assisted laser desorption/ionization) – mass spectrometry (MS), Brunauer–Emmett–Teller (BET) – NLDFT (Non-local Density Functional Theory) method, scanning electron microscopy (SEM), simultaneous TG-DTG and coupled TG-MS. XRD results showed that the average crystallite size of synthesized VD3/PVA/CaCO3 amounts 32.93 nm exhibiting microstrain presence, where PVA incorporation causes non-uniform calcite lattice distortion. SEM analysis showed that VD3/PVA/CaCO3 nanocomposite scaffold contains agglomerated rhomboidal calcite particles with VD3 particles of irregular shapes attached. Fabricated VD3/PVA/CaCO3 clearly showed the existence of calcite “staircase” dendrites as the aftermath of inhibiting the effect of impurities on the growth of crystals in normal directions. It was determined that the decomposition of PVA additionally enhances the thermal stability of VD3, through the stabilization effect by acting on van der Waal’s forces during polyene formation, confirmed by MALDI-TOF MS results.",
journal = "Journal of Materials Science",
title = "Biomineral nanocomposite scaffold (CaCO3/PVA based) carrier for improved stability of vitamin D3: characterization analysis and material properties",
volume = "58",
number = "15",
pages = "6580-6601",
doi = "10.1007/s10853-023-08453-z"
}

Janković, B., Papović, S., Vraneš, M., Knežević, T., Pržulj, S., Zeljković, S., Veličković, S., Veljković, F.,& Jelić, D.. (2023). Biomineral nanocomposite scaffold (CaCO3/PVA based) carrier for improved stability of vitamin D3: characterization analysis and material properties. in Journal of Materials Science, 58(15), 6580-6601.
https://doi.org/10.1007/s10853-023-08453-z

Janković B, Papović S, Vraneš M, Knežević T, Pržulj S, Zeljković S, Veličković S, Veljković F, Jelić D. Biomineral nanocomposite scaffold (CaCO3/PVA based) carrier for improved stability of vitamin D3: characterization analysis and material properties. in Journal of Materials Science. 2023;58(15):6580-6601.
doi:10.1007/s10853-023-08453-z .

Janković, Bojan, Papović, Snežana, Vraneš, Milan, Knežević, Teodora, Pržulj, Sanja, Zeljković, Saša, Veličković, Suzana, Veljković, Filip, Jelić, Dijana, "Biomineral nanocomposite scaffold (CaCO3/PVA based) carrier for improved stability of vitamin D3: characterization analysis and material properties" in Journal of Materials Science, 58, no. 15 (2023):6580-6601,
https://doi.org/10.1007/s10853-023-08453-z . .

TY  - JOUR
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Matović, Valentina
AU  - Srbovan, Maja
AU  - Koruga, Đuro
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10854
AB  - Introduction In the present study, we assessed the effects of the hyper-harmonized-hydroxylated fullerene–water complex (3HFWC) on Alzheimer's disease (AD) neuropathological hallmarks in 5XFAD mice, an AD animal model. Methods The 3-week-old 5XFAD mice were exposed to 3HFWC water solution ad libitum for 3 months in the presymptomatic phase of pathology. The functional effects of the treatment were confirmed through near-infrared spectroscopy (NIRS) analysis through machine learning (ML) using artificial neural networks (ANNs) to classify the control and 3HFWC-treated brain tissue samples. The effects of 3HFWC treatment on amyloid-β (Aβ) accumulation, plaque formation, gliosis, and synaptic plasticity in cortical and hippocampal tissue were assessed. Results The 3HFWC treatment significantly decreased the amyloid-β plaque load in specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not induce the activation of glia (astrocytes and microglia) nor did it negatively affect synaptic protein markers (GAP-43, synaptophysin, and PSD-95). Conclusion The obtained results point to the potential of 3HFWC, when applied in the presymptomatic phase of AD, to interfere with amyloid plaque formation without inducing AD-related pathological processes such as neuroinflammation, gliosis, and synaptic vulnerability.
T2  - CNS Neuroscience & Therapeutics
T1  - The presymptomatic treatment with 3HFWC nanosubstance decreased plaque load in 5XFAD mouse model of Alzheimer's disease
IS  - InPress
DO  - 10.1111/cns.14188
ER  - 

@article{
author = "Perović, Milka and Ćirić, Jelena and Matović, Valentina and Srbovan, Maja and Koruga, Đuro and Kanazir, Selma and Ivković, Sanja",
year = "2023",
abstract = "Introduction In the present study, we assessed the effects of the hyper-harmonized-hydroxylated fullerene–water complex (3HFWC) on Alzheimer's disease (AD) neuropathological hallmarks in 5XFAD mice, an AD animal model. Methods The 3-week-old 5XFAD mice were exposed to 3HFWC water solution ad libitum for 3 months in the presymptomatic phase of pathology. The functional effects of the treatment were confirmed through near-infrared spectroscopy (NIRS) analysis through machine learning (ML) using artificial neural networks (ANNs) to classify the control and 3HFWC-treated brain tissue samples. The effects of 3HFWC treatment on amyloid-β (Aβ) accumulation, plaque formation, gliosis, and synaptic plasticity in cortical and hippocampal tissue were assessed. Results The 3HFWC treatment significantly decreased the amyloid-β plaque load in specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not induce the activation of glia (astrocytes and microglia) nor did it negatively affect synaptic protein markers (GAP-43, synaptophysin, and PSD-95). Conclusion The obtained results point to the potential of 3HFWC, when applied in the presymptomatic phase of AD, to interfere with amyloid plaque formation without inducing AD-related pathological processes such as neuroinflammation, gliosis, and synaptic vulnerability.",
journal = "CNS Neuroscience & Therapeutics",
title = "The presymptomatic treatment with 3HFWC nanosubstance decreased plaque load in 5XFAD mouse model of Alzheimer's disease",
number = "InPress",
doi = "10.1111/cns.14188"
}

Perović, M., Ćirić, J., Matović, V., Srbovan, M., Koruga, Đ., Kanazir, S.,& Ivković, S.. (2023). The presymptomatic treatment with 3HFWC nanosubstance decreased plaque load in 5XFAD mouse model of Alzheimer's disease. in CNS Neuroscience & Therapeutics(InPress).
https://doi.org/10.1111/cns.14188

Perović M, Ćirić J, Matović V, Srbovan M, Koruga Đ, Kanazir S, Ivković S. The presymptomatic treatment with 3HFWC nanosubstance decreased plaque load in 5XFAD mouse model of Alzheimer's disease. in CNS Neuroscience & Therapeutics. 2023;(InPress).
doi:10.1111/cns.14188 .

Perović, Milka, Ćirić, Jelena, Matović, Valentina, Srbovan, Maja, Koruga, Đuro, Kanazir, Selma, Ivković, Sanja, "The presymptomatic treatment with 3HFWC nanosubstance decreased plaque load in 5XFAD mouse model of Alzheimer's disease" in CNS Neuroscience & Therapeutics, no. InPress (2023),
https://doi.org/10.1111/cns.14188 . .

TY  - JOUR
AU  - Momčilović, Miloš
AU  - Petrović, Jelena
AU  - Nemoda, Milica
AU  - Ciganović, Jovan
AU  - Krstulović, Nikša
AU  - Ognjanović, Miloš
AU  - Živković, Sanja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10859
AB  - Pulsed laser ablation in liquids (PLAL) is an approach for the direct synthesis of nanoparticles from the bulk material. In the present work, silver and gold nanoparticles (NPs) were synthesized using the PLAL technique, and obtained water colloid suspensions were characterized by TEM–EDX, ICP-OES, UV–VIS, and DLS methods. On the other hand, Laser-Induced Breakdown Spectroscopy (LIBS) is a well-recognized and versatile analytical technique for the element analysis of solid samples. However, obtaining improved spectral intensity and detection sensitivity are still great challenging tasks, especially for an alternative and cost-effective LIBS setup based on TEA CO2 laser. Considering these demands, this work aimed to investigate a promising approach to signal enhancement based on the deposition of noble NPs on the plastic sample. The effect of NPS on the enhancement of the LIBS signal has been investigated. LIBS experiments were carried out in air at atmospheric pressure and obtained spectra with a high signal-to-background (SBR) ratio. This study shows that signal enhancement can be achieved followed by the lower limits of detection by increasing the ablation amount rate.
T2  - Applied Physics. B: Lasers and Optics
T1  - Laser ablation in water for silver and gold nanoparticle synthesis and their application for improvement of TEA CO2 LIBS setup performance
VL  - 129
IS  - 4
SP  - 62
DO  - 10.1007/s00340-023-08007-w
ER  - 

@article{
author = "Momčilović, Miloš and Petrović, Jelena and Nemoda, Milica and Ciganović, Jovan and Krstulović, Nikša and Ognjanović, Miloš and Živković, Sanja",
year = "2023",
abstract = "Pulsed laser ablation in liquids (PLAL) is an approach for the direct synthesis of nanoparticles from the bulk material. In the present work, silver and gold nanoparticles (NPs) were synthesized using the PLAL technique, and obtained water colloid suspensions were characterized by TEM–EDX, ICP-OES, UV–VIS, and DLS methods. On the other hand, Laser-Induced Breakdown Spectroscopy (LIBS) is a well-recognized and versatile analytical technique for the element analysis of solid samples. However, obtaining improved spectral intensity and detection sensitivity are still great challenging tasks, especially for an alternative and cost-effective LIBS setup based on TEA CO2 laser. Considering these demands, this work aimed to investigate a promising approach to signal enhancement based on the deposition of noble NPs on the plastic sample. The effect of NPS on the enhancement of the LIBS signal has been investigated. LIBS experiments were carried out in air at atmospheric pressure and obtained spectra with a high signal-to-background (SBR) ratio. This study shows that signal enhancement can be achieved followed by the lower limits of detection by increasing the ablation amount rate.",
journal = "Applied Physics. B: Lasers and Optics",
title = "Laser ablation in water for silver and gold nanoparticle synthesis and their application for improvement of TEA CO2 LIBS setup performance",
volume = "129",
number = "4",
pages = "62",
doi = "10.1007/s00340-023-08007-w"
}

Momčilović, M., Petrović, J., Nemoda, M., Ciganović, J., Krstulović, N., Ognjanović, M.,& Živković, S.. (2023). Laser ablation in water for silver and gold nanoparticle synthesis and their application for improvement of TEA CO2 LIBS setup performance. in Applied Physics. B: Lasers and Optics, 129(4), 62.
https://doi.org/10.1007/s00340-023-08007-w

Momčilović M, Petrović J, Nemoda M, Ciganović J, Krstulović N, Ognjanović M, Živković S. Laser ablation in water for silver and gold nanoparticle synthesis and their application for improvement of TEA CO2 LIBS setup performance. in Applied Physics. B: Lasers and Optics. 2023;129(4):62.
doi:10.1007/s00340-023-08007-w .

Momčilović, Miloš, Petrović, Jelena, Nemoda, Milica, Ciganović, Jovan, Krstulović, Nikša, Ognjanović, Miloš, Živković, Sanja, "Laser ablation in water for silver and gold nanoparticle synthesis and their application for improvement of TEA CO2 LIBS setup performance" in Applied Physics. B: Lasers and Optics, 129, no. 4 (2023):62,
https://doi.org/10.1007/s00340-023-08007-w . .

TY  - JOUR
AU  - Glavonić, Emilija
AU  - Mitić, Miloš
AU  - Francija, Ester
AU  - Petrović, Zorica
AU  - Adžić, Miroslav
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10514
AB  - Adolescence is a key phase of development for perturbations in fear extinction, with inability to adequately manage fear a potent factor for developing psychiatric disorders in adulthood. However, while behavioral correlates of adolescent fear regulation are established to a degree, molecular mediators of extinction learning in adolescence remain largely unknown. In this study, we observed fear acquisition and fear extinction (across 4 and 7 days) of adolescent and adult mice of both sexes and investigated how hippocampal levels of different plasticity markers relate to extinction learning. While fear was acquired evenly in males and females of both ages, fear extinction was found to be impaired in adolescent males. We also observed lower levels of GluA1, GLUN2A and GLUN2B subunits in male adolescents following fear acquisition, with an increase in their expression, as well as the activity of Erk-mTOR pathway over subsequent extinction sessions, which was paralleled with improved extinction learning. On the other hand, we detected no changes in plasticity-related proteins after fear acquisition in females, with alterations in GluA1, GluA4 and GLUN2B levels across fear extinction sessions. Additionally, we did not discern any pattern regarding the Erk-mTOR activity in female mice associated with their extinction performance. Overall, our research identifies sex-specific synaptic properties in the hippocampus that underlie developmentally regulated differences in fear extinction learning. We also point out hippocampal NMDA-Erk-mTOR signaling as the driving force behind successful fear extinction in male adolescents, highlighting this pathway as a potential therapeutic target for fear-related disorders in the adolescent population. © 2022 The Authors
T2  - Brain Research Bulletin
T1  - Sex-specific role of hippocampal NMDA-Erk-mTOR signaling in fear extinction of adolescent mice
VL  - 192
SP  - 156
EP  - 167
DO  - 10.1016/j.brainresbull.2022.11.011
ER  - 

@article{
author = "Glavonić, Emilija and Mitić, Miloš and Francija, Ester and Petrović, Zorica and Adžić, Miroslav",
year = "2023",
abstract = "Adolescence is a key phase of development for perturbations in fear extinction, with inability to adequately manage fear a potent factor for developing psychiatric disorders in adulthood. However, while behavioral correlates of adolescent fear regulation are established to a degree, molecular mediators of extinction learning in adolescence remain largely unknown. In this study, we observed fear acquisition and fear extinction (across 4 and 7 days) of adolescent and adult mice of both sexes and investigated how hippocampal levels of different plasticity markers relate to extinction learning. While fear was acquired evenly in males and females of both ages, fear extinction was found to be impaired in adolescent males. We also observed lower levels of GluA1, GLUN2A and GLUN2B subunits in male adolescents following fear acquisition, with an increase in their expression, as well as the activity of Erk-mTOR pathway over subsequent extinction sessions, which was paralleled with improved extinction learning. On the other hand, we detected no changes in plasticity-related proteins after fear acquisition in females, with alterations in GluA1, GluA4 and GLUN2B levels across fear extinction sessions. Additionally, we did not discern any pattern regarding the Erk-mTOR activity in female mice associated with their extinction performance. Overall, our research identifies sex-specific synaptic properties in the hippocampus that underlie developmentally regulated differences in fear extinction learning. We also point out hippocampal NMDA-Erk-mTOR signaling as the driving force behind successful fear extinction in male adolescents, highlighting this pathway as a potential therapeutic target for fear-related disorders in the adolescent population. © 2022 The Authors",
journal = "Brain Research Bulletin",
title = "Sex-specific role of hippocampal NMDA-Erk-mTOR signaling in fear extinction of adolescent mice",
volume = "192",
pages = "156-167",
doi = "10.1016/j.brainresbull.2022.11.011"
}

Glavonić, E., Mitić, M., Francija, E., Petrović, Z.,& Adžić, M.. (2023). Sex-specific role of hippocampal NMDA-Erk-mTOR signaling in fear extinction of adolescent mice. in Brain Research Bulletin, 192, 156-167.
https://doi.org/10.1016/j.brainresbull.2022.11.011

Glavonić E, Mitić M, Francija E, Petrović Z, Adžić M. Sex-specific role of hippocampal NMDA-Erk-mTOR signaling in fear extinction of adolescent mice. in Brain Research Bulletin. 2023;192:156-167.
doi:10.1016/j.brainresbull.2022.11.011 .

Glavonić, Emilija, Mitić, Miloš, Francija, Ester, Petrović, Zorica, Adžić, Miroslav, "Sex-specific role of hippocampal NMDA-Erk-mTOR signaling in fear extinction of adolescent mice" in Brain Research Bulletin, 192 (2023):156-167,
https://doi.org/10.1016/j.brainresbull.2022.11.011 . .