TY  - JOUR
AU  - Vučić, Vesna
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Radojčić, Marija
AU  - Ruždijić, Sabera
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3467
AB  - The antiproliferative and cytotoxic potential of the nucleotide analog 8-Cl-cAMP was tested in PC-3 and DU145 metastatic human prostate cancer cells. The drug was examined as the only therapeutic agent and in combination with ionizing irradiation (IR). Highly synergistic effects of IR and 8-Cl-cAMP were observed in both cell lines when examined by the MTT viability and BrdU proliferation assays. The combination of IR and 8-Cl-cAMP at clinically relevant doses exerted substantial growth inhibition. The combination of IR and 8-Cl-cAMP caused a significant disturbance in the distribution of cell cycle phases. Cell cycle arrest in the sub-G0/G1 phase predominated in both cell lines. The most striking observation was a significant increase in apoptotic PC-3 and DU145 cells. The DU145 cells were three times more sensitive to the combined treatment than PC-3 cells. The initial resistance to IR-induced apoptosis in these p53-deficient prostate cancer cell lines was overcome through an alternative proapoptotic pathway induced by 8-Cl-cAMP. Considering the low effective doses of treatments, improved tumor eradication rates and minimal undesirable side effects, the combination of IR and 8-Cl-cAMP could be the therapy of choice in treating prostate cancer.
T2  - Investigational New Drugs
T1  - The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells
VL  - 26
IS  - 4
SP  - 309
EP  - 317
DO  - 10.1007/s10637-007-9101-4
ER  - 

@article{
author = "Vučić, Vesna and Nićiforović, Ana and Adžić, Miroslav and Radojčić, Marija and Ruždijić, Sabera",
year = "2008",
abstract = "The antiproliferative and cytotoxic potential of the nucleotide analog 8-Cl-cAMP was tested in PC-3 and DU145 metastatic human prostate cancer cells. The drug was examined as the only therapeutic agent and in combination with ionizing irradiation (IR). Highly synergistic effects of IR and 8-Cl-cAMP were observed in both cell lines when examined by the MTT viability and BrdU proliferation assays. The combination of IR and 8-Cl-cAMP at clinically relevant doses exerted substantial growth inhibition. The combination of IR and 8-Cl-cAMP caused a significant disturbance in the distribution of cell cycle phases. Cell cycle arrest in the sub-G0/G1 phase predominated in both cell lines. The most striking observation was a significant increase in apoptotic PC-3 and DU145 cells. The DU145 cells were three times more sensitive to the combined treatment than PC-3 cells. The initial resistance to IR-induced apoptosis in these p53-deficient prostate cancer cell lines was overcome through an alternative proapoptotic pathway induced by 8-Cl-cAMP. Considering the low effective doses of treatments, improved tumor eradication rates and minimal undesirable side effects, the combination of IR and 8-Cl-cAMP could be the therapy of choice in treating prostate cancer.",
journal = "Investigational New Drugs",
title = "The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells",
volume = "26",
number = "4",
pages = "309-317",
doi = "10.1007/s10637-007-9101-4"
}

Vučić, V., Nićiforović, A., Adžić, M., Radojčić, M.,& Ruždijić, S.. (2008). The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells. in Investigational New Drugs, 26(4), 309-317.
https://doi.org/10.1007/s10637-007-9101-4

Vučić V, Nićiforović A, Adžić M, Radojčić M, Ruždijić S. The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells. in Investigational New Drugs. 2008;26(4):309-317.
doi:10.1007/s10637-007-9101-4 .

Vučić, Vesna, Nićiforović, Ana, Adžić, Miroslav, Radojčić, Marija, Ruždijić, Sabera, "The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells" in Investigational New Drugs, 26, no. 4 (2008):309-317,
https://doi.org/10.1007/s10637-007-9101-4 . .

TY  - JOUR
AU  - Nićiforović, Ana
AU  - Đorđević, Jelena D.
AU  - Adžić, Miroslav
AU  - Vučić, Vesna
AU  - Mitrasinovic, Petar M.
AU  - Radojčić, Marija
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3404
AB  - Molecular mechanisms for the gamma-ionizing radiation (IR) resistance of human prostate cancer cells, PC-3, are not quite clear. Since the low-LET-IR effects are primarily manifested by the generation of reactive oxygen species (ROS), the IR-induced expressions both of ROS-metabolizing antioxidant enzymes, such as Mn- and CuZn superoxide dismutases (SODs) and catalase (Cat), and of the transcriptional nuclear factor-kappaB (NF-kappa B) were explored. A substantial increase in the concentrations of SODs was observed in the cells irradiated by 10 and 20 Gy relative to those irradiated by 0 and 2 Gy, while the Cat and NF-kappa B expressions were found to be fairly stable. A system biology model was developed to shed more light on how MnSOD affects the biological state of cells depending upon the production of H2O2. By raising the initial presence of MnSOD in the 0.7-10 mu M concentration range, the time-dependent concentrations of H2O2 for various initial levels of MnSOD were contrasted. The radioresistance of PC-3 cells is suggested to be associated with the positive, feed-forward vicious circle established between the H2O2-mediated elevation of MnSOD expression.
T2  - Annals of Biomedical Engineering
T1  - Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells
VL  - 36
IS  - 5
SP  - 831
EP  - 838
DO  - 10.1007/s10439-008-9457-4
ER  - 

@article{
author = "Nićiforović, Ana and Đorđević, Jelena D. and Adžić, Miroslav and Vučić, Vesna and Mitrasinovic, Petar M. and Radojčić, Marija",
year = "2008",
abstract = "Molecular mechanisms for the gamma-ionizing radiation (IR) resistance of human prostate cancer cells, PC-3, are not quite clear. Since the low-LET-IR effects are primarily manifested by the generation of reactive oxygen species (ROS), the IR-induced expressions both of ROS-metabolizing antioxidant enzymes, such as Mn- and CuZn superoxide dismutases (SODs) and catalase (Cat), and of the transcriptional nuclear factor-kappaB (NF-kappa B) were explored. A substantial increase in the concentrations of SODs was observed in the cells irradiated by 10 and 20 Gy relative to those irradiated by 0 and 2 Gy, while the Cat and NF-kappa B expressions were found to be fairly stable. A system biology model was developed to shed more light on how MnSOD affects the biological state of cells depending upon the production of H2O2. By raising the initial presence of MnSOD in the 0.7-10 mu M concentration range, the time-dependent concentrations of H2O2 for various initial levels of MnSOD were contrasted. The radioresistance of PC-3 cells is suggested to be associated with the positive, feed-forward vicious circle established between the H2O2-mediated elevation of MnSOD expression.",
journal = "Annals of Biomedical Engineering",
title = "Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells",
volume = "36",
number = "5",
pages = "831-838",
doi = "10.1007/s10439-008-9457-4"
}

Nićiforović, A., Đorđević, J. D., Adžić, M., Vučić, V., Mitrasinovic, P. M.,& Radojčić, M.. (2008). Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells. in Annals of Biomedical Engineering, 36(5), 831-838.
https://doi.org/10.1007/s10439-008-9457-4

Nićiforović A, Đorđević JD, Adžić M, Vučić V, Mitrasinovic PM, Radojčić M. Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells. in Annals of Biomedical Engineering. 2008;36(5):831-838.
doi:10.1007/s10439-008-9457-4 .

Nićiforović, Ana, Đorđević, Jelena D., Adžić, Miroslav, Vučić, Vesna, Mitrasinovic, Petar M., Radojčić, Marija, "Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells" in Annals of Biomedical Engineering, 36, no. 5 (2008):831-838,
https://doi.org/10.1007/s10439-008-9457-4 . .