TY  - JOUR
AU  - Vukojević, Vesna
AU  - Đurđić, Slađana
AU  - Švorc, Lubomir
AU  - Ćirković Veličković, Tanja
AU  - Mutić, Jelena J.
AU  - Stanković, Dalibor M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s12161-018-1249-3
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7919
AB  - Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 mu M was estimated, with limit of detection of 0.7 mu M, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with satisfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.
T2  - Food Analytical Methods
T1  - Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents
VL  - 11
IS  - 9
SP  - 2590
EP  - 2596
DO  - 10.1007/s12161-018-1249-3
ER  - 

@article{
author = "Vukojević, Vesna and Đurđić, Slađana and Švorc, Lubomir and Ćirković Veličković, Tanja and Mutić, Jelena J. and Stanković, Dalibor M.",
year = "2018",
abstract = "Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 mu M was estimated, with limit of detection of 0.7 mu M, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with satisfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.",
journal = "Food Analytical Methods",
title = "Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents",
volume = "11",
number = "9",
pages = "2590-2596",
doi = "10.1007/s12161-018-1249-3"
}

Vukojević, V., Đurđić, S., Švorc, L., Ćirković Veličković, T., Mutić, J. J.,& Stanković, D. M.. (2018). Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents. in Food Analytical Methods, 11(9), 2590-2596.
https://doi.org/10.1007/s12161-018-1249-3

Vukojević V, Đurđić S, Švorc L, Ćirković Veličković T, Mutić JJ, Stanković DM. Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents. in Food Analytical Methods. 2018;11(9):2590-2596.
doi:10.1007/s12161-018-1249-3 .

Vukojević, Vesna, Đurđić, Slađana, Švorc, Lubomir, Ćirković Veličković, Tanja, Mutić, Jelena J., Stanković, Dalibor M., "Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents" in Food Analytical Methods, 11, no. 9 (2018):2590-2596,
https://doi.org/10.1007/s12161-018-1249-3 . .