TY  - JOUR
AU  - Stanojević, Boban
AU  - Osiowy, Carla
AU  - Schaefer, Stephan
AU  - Bojovic, Ksenija
AU  - Blagojevic, Jelena
AU  - Nesic, Milica
AU  - Yamashita, Shunichi
AU  - Stamenković, Gorana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4450
AB  - Hepatitis B virus (HBV) is classified into 8 genotypes with distinct geographical distribution. Genotype D (HBV/D) has the widest distribution area and is comprised of 7 subgenotypes. Subgenotypes D1, D2 and D3 appear worldwide, while D4-D7 have a more restricted distribution. Within the Mediterranean area, HBV/D and subgenotype D3 are the most prevalent. The purpose of this study was to characterize the full genome of Serbian HBV/D3 isolates by comparison and phylogenetic analysis with HBV/D3 sequences (66 samples) found in GeneBank/DDBJ databases from different parts of the world. Isolates were obtained from three patients diagnosed with chronic hepatitis B (HBsAg +). All three isolates have two very rare nucleotide substitutions, A929T and T150A, which indicate the same ancestor. Phylogenetic analysis of HBV/D3 genome sequences throughout the world follows an ethno-geographical origin of isolates with rare exceptions, which could be explained by human travelling and migration. The geographically close but ethnically different Serbian and Italian isolates clustered in the same subnode, and on a common branch with strains from Northern Canada. To test the apparently close HBV phylogenetic relationship between completely separated patients from Serbia and Northern Canada we analyzed in depth a 440 bp region of the HBsAg from Canadian (n = 73) and Serbian (n = 70) isolates. The constructed parsimony tree revealed that strains from Serbia and Northern Canada fell along the same branch which indicates independent evolution within regions of each country, Considering that HBsAg sequence has limited variability for phylogenetic analyses, our hypothesis needs further confirmation with more HBV complete genome sequences. (C) 2011 Elsevier B.V. All rights reserved,
T2  - Infection Genetics and Evolution
T1  - Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia
VL  - 11
IS  - 6
SP  - 1475
EP  - 1480
DO  - 10.1016/j.meegid.2011.05.004
ER  - 

@article{
author = "Stanojević, Boban and Osiowy, Carla and Schaefer, Stephan and Bojovic, Ksenija and Blagojevic, Jelena and Nesic, Milica and Yamashita, Shunichi and Stamenković, Gorana",
year = "2011",
abstract = "Hepatitis B virus (HBV) is classified into 8 genotypes with distinct geographical distribution. Genotype D (HBV/D) has the widest distribution area and is comprised of 7 subgenotypes. Subgenotypes D1, D2 and D3 appear worldwide, while D4-D7 have a more restricted distribution. Within the Mediterranean area, HBV/D and subgenotype D3 are the most prevalent. The purpose of this study was to characterize the full genome of Serbian HBV/D3 isolates by comparison and phylogenetic analysis with HBV/D3 sequences (66 samples) found in GeneBank/DDBJ databases from different parts of the world. Isolates were obtained from three patients diagnosed with chronic hepatitis B (HBsAg +). All three isolates have two very rare nucleotide substitutions, A929T and T150A, which indicate the same ancestor. Phylogenetic analysis of HBV/D3 genome sequences throughout the world follows an ethno-geographical origin of isolates with rare exceptions, which could be explained by human travelling and migration. The geographically close but ethnically different Serbian and Italian isolates clustered in the same subnode, and on a common branch with strains from Northern Canada. To test the apparently close HBV phylogenetic relationship between completely separated patients from Serbia and Northern Canada we analyzed in depth a 440 bp region of the HBsAg from Canadian (n = 73) and Serbian (n = 70) isolates. The constructed parsimony tree revealed that strains from Serbia and Northern Canada fell along the same branch which indicates independent evolution within regions of each country, Considering that HBsAg sequence has limited variability for phylogenetic analyses, our hypothesis needs further confirmation with more HBV complete genome sequences. (C) 2011 Elsevier B.V. All rights reserved,",
journal = "Infection Genetics and Evolution",
title = "Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia",
volume = "11",
number = "6",
pages = "1475-1480",
doi = "10.1016/j.meegid.2011.05.004"
}

Stanojević, B., Osiowy, C., Schaefer, S., Bojovic, K., Blagojevic, J., Nesic, M., Yamashita, S.,& Stamenković, G.. (2011). Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia. in Infection Genetics and Evolution, 11(6), 1475-1480.
https://doi.org/10.1016/j.meegid.2011.05.004

Stanojević B, Osiowy C, Schaefer S, Bojovic K, Blagojevic J, Nesic M, Yamashita S, Stamenković G. Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia. in Infection Genetics and Evolution. 2011;11(6):1475-1480.
doi:10.1016/j.meegid.2011.05.004 .

Stanojević, Boban, Osiowy, Carla, Schaefer, Stephan, Bojovic, Ksenija, Blagojevic, Jelena, Nesic, Milica, Yamashita, Shunichi, Stamenković, Gorana, "Molecular characterization and phylogenetic analysis of full-genome HBV subgenotype D3 sequences from Serbia" in Infection Genetics and Evolution, 11, no. 6 (2011):1475-1480,
https://doi.org/10.1016/j.meegid.2011.05.004 . .