TY  - JOUR
AU  - Milošević-Đorđević, Olivera
AU  - Grujičić, Darko V.
AU  - Joksić, Gordana
AU  - Marinković, Dragoslav M.
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4306
AB  - The aim of this study was to investigate the genotoxic effects of ritodrine and verapamil on human peripheral lymphocytes in vitro using micronucleus (MN) test. Also, fluorescence in situ hybridization (FISH) with a centromeric probe was performed to determine the origin of the induced MN. Cells were treated with 8.4 x 10(-6) M - 25.2 x 10(-4) M concentrations for ritodrine and 0.56-11 x 10(-5) M concentrations for verapamil, separately and combined. The MN frequencies showed increase after all treatments, but the difference between treated cells and untreated controls were found to be statistically significant only in the concentration range from 8.4 x 10(-5) M - 4.5 x 10(-4) M for ritodrine, 1.1 - 3.3 x 10(-5) M for verapamil, and in combined treatment with concentrations 8.4 x 10(-5) M + 1.1 x 10(-5) M for ritodrine and verapamil. The highest tested concentrations of both medicaments showed cytotoxic effect. Both medicaments decreased the nuclear division index (NDI) in tested concentrations. The results of FISH analysis suggest that verapamil, separately or combined with ritodrine, shows to a larger extent aneugenic than clastogenic effect.
T2  - Human and Experimental Toxicology
T1  - In vitro evaluation of the genotoxicity of ritodrine and verapamil in human lymphocytes
VL  - 30
IS  - 5
SP  - 398
EP  - 405
DO  - 10.1177/0960327110372404
ER  - 

@article{
author = "Milošević-Đorđević, Olivera and Grujičić, Darko V. and Joksić, Gordana and Marinković, Dragoslav M.",
year = "2011",
abstract = "The aim of this study was to investigate the genotoxic effects of ritodrine and verapamil on human peripheral lymphocytes in vitro using micronucleus (MN) test. Also, fluorescence in situ hybridization (FISH) with a centromeric probe was performed to determine the origin of the induced MN. Cells were treated with 8.4 x 10(-6) M - 25.2 x 10(-4) M concentrations for ritodrine and 0.56-11 x 10(-5) M concentrations for verapamil, separately and combined. The MN frequencies showed increase after all treatments, but the difference between treated cells and untreated controls were found to be statistically significant only in the concentration range from 8.4 x 10(-5) M - 4.5 x 10(-4) M for ritodrine, 1.1 - 3.3 x 10(-5) M for verapamil, and in combined treatment with concentrations 8.4 x 10(-5) M + 1.1 x 10(-5) M for ritodrine and verapamil. The highest tested concentrations of both medicaments showed cytotoxic effect. Both medicaments decreased the nuclear division index (NDI) in tested concentrations. The results of FISH analysis suggest that verapamil, separately or combined with ritodrine, shows to a larger extent aneugenic than clastogenic effect.",
journal = "Human and Experimental Toxicology",
title = "In vitro evaluation of the genotoxicity of ritodrine and verapamil in human lymphocytes",
volume = "30",
number = "5",
pages = "398-405",
doi = "10.1177/0960327110372404"
}

Milošević-Đorđević, O., Grujičić, D. V., Joksić, G.,& Marinković, D. M.. (2011). In vitro evaluation of the genotoxicity of ritodrine and verapamil in human lymphocytes. in Human and Experimental Toxicology, 30(5), 398-405.
https://doi.org/10.1177/0960327110372404

Milošević-Đorđević O, Grujičić DV, Joksić G, Marinković DM. In vitro evaluation of the genotoxicity of ritodrine and verapamil in human lymphocytes. in Human and Experimental Toxicology. 2011;30(5):398-405.
doi:10.1177/0960327110372404 .

Milošević-Đorđević, Olivera, Grujičić, Darko V., Joksić, Gordana, Marinković, Dragoslav M., "In vitro evaluation of the genotoxicity of ritodrine and verapamil in human lymphocytes" in Human and Experimental Toxicology, 30, no. 5 (2011):398-405,
https://doi.org/10.1177/0960327110372404 . .