TY  - JOUR
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Zarić, Božidarka
AU  - Nešković-Konstantinović, Zora
AU  - Spasić, Snežana D.
AU  - Jones, David R.
AU  - Radojčić, Marija
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3490
AB  - To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-kappa B and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4(+) lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P LT 0.001) and after (P LT 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P LT 0.001] and 9 Gy [P LT 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4(+) cells was significantly (P LT 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4(+) cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour.
T2  - Redox Report
T1  - Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects
VL  - 13
IS  - 1
SP  - 17
EP  - 22
DO  - 10.1179/135100008X259088
ER  - 

@article{
author = "Adžić, Miroslav and Nićiforović, Ana and Zarić, Božidarka and Nešković-Konstantinović, Zora and Spasić, Snežana D. and Jones, David R. and Radojčić, Marija",
year = "2008",
abstract = "To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-kappa B and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4(+) lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P LT 0.001) and after (P LT 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P LT 0.001] and 9 Gy [P LT 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4(+) cells was significantly (P LT 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4(+) cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour.",
journal = "Redox Report",
title = "Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects",
volume = "13",
number = "1",
pages = "17-22",
doi = "10.1179/135100008X259088"
}

Adžić, M., Nićiforović, A., Zarić, B., Nešković-Konstantinović, Z., Spasić, S. D., Jones, D. R.,& Radojčić, M.. (2008). Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects. in Redox Report, 13(1), 17-22.
https://doi.org/10.1179/135100008X259088

Adžić M, Nićiforović A, Zarić B, Nešković-Konstantinović Z, Spasić SD, Jones DR, Radojčić M. Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects. in Redox Report. 2008;13(1):17-22.
doi:10.1179/135100008X259088 .

Adžić, Miroslav, Nićiforović, Ana, Zarić, Božidarka, Nešković-Konstantinović, Zora, Spasić, Snežana D., Jones, David R., Radojčić, Marija, "Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects" in Redox Report, 13, no. 1 (2008):17-22,
https://doi.org/10.1179/135100008X259088 . .