TY  - JOUR
AU  - Aljancic, Ivana
AU  - Stanković, Miroslava
AU  - Tešević, Vele V.
AU  - Vujisić, Ljubodrag
AU  - Vajs, Vlatka
AU  - Milosavljević, Slobodan
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4002
AB  - This study was conducted to elucidate the in vitro protective effect of five flavonoids [apigenin (1), apigenin-7-O-glucoside (2), centaureidin (3), jaceidin (4) and quercetin (5)] against chromosomal damage in mitogen-induced human lymphocytes. Using the Cytochalasin-B blocked micronucleus (CBMN) assay, in which the biomarker of chromosome breakage and/or chromosome loss is the elevated frequency of micronucleus (MN) in binucleated (BN) cells, the presence of flavonoid 2 in minimal concentration (3 mu g/mL) gave a 35.5% decrease M the frequency of MN when compared with control human lymphocytes. The same concentration of flavonoids 1, 3 and 4, reduced the MN frequency by 24.4%, 28.0% and 28.0%, respectively. Higher concentrations (6 mu g/mL and 10 mu g/mL) seemed less effective. Flavonoid 5 (3 mu g/mL.) induced a slight decrease in MN frequency (5%), while higher doses (6 mu g/mL and 10 mu g/mL) provoked an increase of DNA damage. The comparable values for the cytokinesis-block proliferation index (CBPI) of the tested flavonoids and positive control suggested an inhibitory effect on lymphocyte proliferation. In the DPPH scavenging assay, flavonoids 1-4 demonstrated modest activity, in a dose-dependent manner, compared with the synthetic antioxidants BHT and Trolox, while 5 exhibited comparably high antioxidative activity.
T2  - Natural Product Communications
T1  - Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species
VL  - 5
IS  - 5
SP  - 729
EP  - 732
UR  - https://hdl.handle.net/21.15107/rcub_vinar_4002
ER  - 

@article{
author = "Aljancic, Ivana and Stanković, Miroslava and Tešević, Vele V. and Vujisić, Ljubodrag and Vajs, Vlatka and Milosavljević, Slobodan",
year = "2010",
abstract = "This study was conducted to elucidate the in vitro protective effect of five flavonoids [apigenin (1), apigenin-7-O-glucoside (2), centaureidin (3), jaceidin (4) and quercetin (5)] against chromosomal damage in mitogen-induced human lymphocytes. Using the Cytochalasin-B blocked micronucleus (CBMN) assay, in which the biomarker of chromosome breakage and/or chromosome loss is the elevated frequency of micronucleus (MN) in binucleated (BN) cells, the presence of flavonoid 2 in minimal concentration (3 mu g/mL) gave a 35.5% decrease M the frequency of MN when compared with control human lymphocytes. The same concentration of flavonoids 1, 3 and 4, reduced the MN frequency by 24.4%, 28.0% and 28.0%, respectively. Higher concentrations (6 mu g/mL and 10 mu g/mL) seemed less effective. Flavonoid 5 (3 mu g/mL.) induced a slight decrease in MN frequency (5%), while higher doses (6 mu g/mL and 10 mu g/mL) provoked an increase of DNA damage. The comparable values for the cytokinesis-block proliferation index (CBPI) of the tested flavonoids and positive control suggested an inhibitory effect on lymphocyte proliferation. In the DPPH scavenging assay, flavonoids 1-4 demonstrated modest activity, in a dose-dependent manner, compared with the synthetic antioxidants BHT and Trolox, while 5 exhibited comparably high antioxidative activity.",
journal = "Natural Product Communications",
title = "Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species",
volume = "5",
number = "5",
pages = "729-732",
url = "https://hdl.handle.net/21.15107/rcub_vinar_4002"
}

Aljancic, I., Stanković, M., Tešević, V. V., Vujisić, L., Vajs, V.,& Milosavljević, S.. (2010). Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species. in Natural Product Communications, 5(5), 729-732.
https://hdl.handle.net/21.15107/rcub_vinar_4002

Aljancic I, Stanković M, Tešević VV, Vujisić L, Vajs V, Milosavljević S. Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species. in Natural Product Communications. 2010;5(5):729-732.
https://hdl.handle.net/21.15107/rcub_vinar_4002 .

Aljancic, Ivana, Stanković, Miroslava, Tešević, Vele V., Vujisić, Ljubodrag, Vajs, Vlatka, Milosavljević, Slobodan, "Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species" in Natural Product Communications, 5, no. 5 (2010):729-732,
https://hdl.handle.net/21.15107/rcub_vinar_4002 .