TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Keta, Otilija D.
AU  - Iannolo, Gioacchin
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6905
AB  - Response of human HTB140 melanoma cells to proton irradiation in combination with fotemustine (FM) was investigated. Effects of these agents were analysed on cell proliferation and induction of apoptosis. Cells pretreated with 100- or 250-mu M of FM were irradiated in the middle of the therapeutic 62-MeV proton spread-out Bragg peak, with a dose of 16 Gy. All treatments reduced proliferation and survival of melanoma cells. The most pronounced effects of the combined treatment were obtained for cell survivals. The level of apoptosis increased after all applied treatments. Particularly good pro-apoptotic effect was achieved when proton irradiation was combined with 250 mu M of FM. This was followed by the increased expression of p53 gene. The obtained results have shown that combined application of FM and protons significantly reduced growth of this resistant melanoma cell line.
T2  - Radiation Protection Dosimetry
T1  - Proton inactivation of melanoma cells enhanced by fotemustine
VL  - 143
IS  - 2-4
SP  - 503
EP  - 507
DO  - 10.1093/rpd/ncq527
ER  - 

@article{
author = "Ristić-Fira, Aleksandra and Korićanac, Lela and Žakula, Jelena and Keta, Otilija D. and Iannolo, Gioacchin and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2011",
abstract = "Response of human HTB140 melanoma cells to proton irradiation in combination with fotemustine (FM) was investigated. Effects of these agents were analysed on cell proliferation and induction of apoptosis. Cells pretreated with 100- or 250-mu M of FM were irradiated in the middle of the therapeutic 62-MeV proton spread-out Bragg peak, with a dose of 16 Gy. All treatments reduced proliferation and survival of melanoma cells. The most pronounced effects of the combined treatment were obtained for cell survivals. The level of apoptosis increased after all applied treatments. Particularly good pro-apoptotic effect was achieved when proton irradiation was combined with 250 mu M of FM. This was followed by the increased expression of p53 gene. The obtained results have shown that combined application of FM and protons significantly reduced growth of this resistant melanoma cell line.",
journal = "Radiation Protection Dosimetry",
title = "Proton inactivation of melanoma cells enhanced by fotemustine",
volume = "143",
number = "2-4",
pages = "503-507",
doi = "10.1093/rpd/ncq527"
}

Ristić-Fira, A., Korićanac, L., Žakula, J., Keta, O. D., Iannolo, G., Cuttone, G.,& Petrović, I. M.. (2011). Proton inactivation of melanoma cells enhanced by fotemustine. in Radiation Protection Dosimetry, 143(2-4), 503-507.
https://doi.org/10.1093/rpd/ncq527

Ristić-Fira A, Korićanac L, Žakula J, Keta OD, Iannolo G, Cuttone G, Petrović IM. Proton inactivation of melanoma cells enhanced by fotemustine. in Radiation Protection Dosimetry. 2011;143(2-4):503-507.
doi:10.1093/rpd/ncq527 .

Ristić-Fira, Aleksandra, Korićanac, Lela, Žakula, Jelena, Keta, Otilija D., Iannolo, Gioacchin, Cuttone, Giacomo, Petrović, Ivan M., "Proton inactivation of melanoma cells enhanced by fotemustine" in Radiation Protection Dosimetry, 143, no. 2-4 (2011):503-507,
https://doi.org/10.1093/rpd/ncq527 . .

TY  - JOUR
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Korićanac, Lela
AU  - Valastro, Lucia
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4101
AB  - Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was at its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer.
T2  - International Journal of Radiation Biology
T1  - Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak
VL  - 86
IS  - 9
SP  - 742
EP  - 751
DO  - 10.3109/09553002.2010.481322
ER  - 

@article{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V. and Korićanac, Lela and Valastro, Lucia and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo",
year = "2010",
abstract = "Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was at its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer.",
journal = "International Journal of Radiation Biology",
title = "Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak",
volume = "86",
number = "9",
pages = "742-751",
doi = "10.3109/09553002.2010.481322"
}

Petrović, I. M., Ristić-Fira, A., Todorović, D. V., Korićanac, L., Valastro, L., Cirrone, G. A. P.,& Cuttone, G.. (2010). Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak. in International Journal of Radiation Biology, 86(9), 742-751.
https://doi.org/10.3109/09553002.2010.481322

Petrović IM, Ristić-Fira A, Todorović DV, Korićanac L, Valastro L, Cirrone GAP, Cuttone G. Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak. in International Journal of Radiation Biology. 2010;86(9):742-751.
doi:10.3109/09553002.2010.481322 .

Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., Korićanac, Lela, Valastro, Lucia, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak" in International Journal of Radiation Biology, 86, no. 9 (2010):742-751,
https://doi.org/10.3109/09553002.2010.481322 . .