TY  - JOUR
AU  - Rašić-Marković, Aleksandra
AU  - Stanojlović, Olivera
AU  - Hrnčić, Dragan
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Šušić, Veselinka
AU  - Radosavljevic, Tatjana
AU  - Đurić, Dragan M.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3709
AB  - Hyperhomocysteinemia is associated with various pathologies including cardiovascular disease, stroke, and cognitive dysfunctions. Systemic administration of homocysteine can trigger seizures in animals, and patients with homocystinuria suffer from epileptic seizures. Available data suggest that homocysteine can be harmful to human cells because of its metabolic conversion to homocysteine thiolactone, a reactive thioester. A number of reports have demonstrated a reduction of Na+/K+-ATPase activity in cerebral ischemia, epilepsy and neurodegeneration possibly associated with excitotoxic mechanisms. The aim of this study was to examine the in vivo effects of d,l-homocysteine and d,l-homocysteine thiolactone on Na+/K+- and Mg2+-ATPase activities in erythrocyte (RBC), brain cortex, hippocampus, and brain stem of adult male rats. Our results demonstrate a moderate inhibition of rat hippocampal Na+/K+-ATPase activity by d,l-homocysteine, which however expressed no effect on the activity of this enzyme in the cortex and brain stem. In contrast,d,l-homocysteine thiolactone strongly inhibited Na+/K+-ATPase activity in cortex, hippocampus and brain stem of rats. RBC Na+/K+-ATPase and Mg2+-ATPase activities were not affected by d,l-homocysteine, while d,l-homocysteine thiolactone inhibited only Na+/K+-ATPase activity. This study results show that homocysteine thiolactone significantly inhibits Na+/K+-ATPase activity in the cortex, hippocampus, and brain stem, which may contribute at least in part to the understanding of excitotoxic and convulsive properties of this substance.
T2  - Molecular and Cellular Biochemistry
T1  - The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration
VL  - 327
IS  - 1-2
SP  - 39
EP  - 45
DO  - 10.1007/s11010-009-0040-6
ER  - 

@article{
author = "Rašić-Marković, Aleksandra and Stanojlović, Olivera and Hrnčić, Dragan and Krstić, Danijela Z. and Čolović, Mirjana B. and Šušić, Veselinka and Radosavljevic, Tatjana and Đurić, Dragan M.",
year = "2009",
abstract = "Hyperhomocysteinemia is associated with various pathologies including cardiovascular disease, stroke, and cognitive dysfunctions. Systemic administration of homocysteine can trigger seizures in animals, and patients with homocystinuria suffer from epileptic seizures. Available data suggest that homocysteine can be harmful to human cells because of its metabolic conversion to homocysteine thiolactone, a reactive thioester. A number of reports have demonstrated a reduction of Na+/K+-ATPase activity in cerebral ischemia, epilepsy and neurodegeneration possibly associated with excitotoxic mechanisms. The aim of this study was to examine the in vivo effects of d,l-homocysteine and d,l-homocysteine thiolactone on Na+/K+- and Mg2+-ATPase activities in erythrocyte (RBC), brain cortex, hippocampus, and brain stem of adult male rats. Our results demonstrate a moderate inhibition of rat hippocampal Na+/K+-ATPase activity by d,l-homocysteine, which however expressed no effect on the activity of this enzyme in the cortex and brain stem. In contrast,d,l-homocysteine thiolactone strongly inhibited Na+/K+-ATPase activity in cortex, hippocampus and brain stem of rats. RBC Na+/K+-ATPase and Mg2+-ATPase activities were not affected by d,l-homocysteine, while d,l-homocysteine thiolactone inhibited only Na+/K+-ATPase activity. This study results show that homocysteine thiolactone significantly inhibits Na+/K+-ATPase activity in the cortex, hippocampus, and brain stem, which may contribute at least in part to the understanding of excitotoxic and convulsive properties of this substance.",
journal = "Molecular and Cellular Biochemistry",
title = "The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration",
volume = "327",
number = "1-2",
pages = "39-45",
doi = "10.1007/s11010-009-0040-6"
}

Rašić-Marković, A., Stanojlović, O., Hrnčić, D., Krstić, D. Z., Čolović, M. B., Šušić, V., Radosavljevic, T.,& Đurić, D. M.. (2009). The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration. in Molecular and Cellular Biochemistry, 327(1-2), 39-45.
https://doi.org/10.1007/s11010-009-0040-6

Rašić-Marković A, Stanojlović O, Hrnčić D, Krstić DZ, Čolović MB, Šušić V, Radosavljevic T, Đurić DM. The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration. in Molecular and Cellular Biochemistry. 2009;327(1-2):39-45.
doi:10.1007/s11010-009-0040-6 .

Rašić-Marković, Aleksandra, Stanojlović, Olivera, Hrnčić, Dragan, Krstić, Danijela Z., Čolović, Mirjana B., Šušić, Veselinka, Radosavljevic, Tatjana, Đurić, Dragan M., "The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration" in Molecular and Cellular Biochemistry, 327, no. 1-2 (2009):39-45,
https://doi.org/10.1007/s11010-009-0040-6 . .