Ministry of Science and Technological Development of the Republic of Serbia [142006]

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Ministry of Science and Technological Development of the Republic of Serbia [142006]

Authors

Publications

Dense fine-grained biphasic calcium phosphate (BCP) bioceramics designed by two-step sintering

Lukic, M.; Stojanović, Zoran S.; Škapin, Srečo Davor; Macek-Krzmanc, M.; Mitrić, Miodrag; Markovic, S.; Uskoković, Dragan

(2011)

TY  - JOUR
AU  - Lukic, M.
AU  - Stojanović, Zoran S.
AU  - Škapin, Srečo Davor
AU  - Macek-Krzmanc, M.
AU  - Mitrić, Miodrag
AU  - Markovic, S.
AU  - Uskoković, Dragan
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4155
AB  - In this study, dense fine-grained biphasic calcium phosphate bioceramics were designed via the two-step sintering method The starting powder was nanosized calcium-deficient hydroxyapatite, whose phase composition, average particle size and morphology were characterized by XRD, FTIR, Raman spectroscopy, laser diffraction and FE-SEM The phase transformations of the initial powder during heating up to 1200 degrees C were examined using TG/DSC At first, conventional sintering was performed and the recorded shrinkage/densification data were used to find out the appropriate experimental conditions for two step sintering The obtained results show that two step sintering yields BCP ceramics consisting of hydroxyapatite and beta-TCP with lull dense, homogeneous structure with average grain size of 375 nm Furthermore BCP ceramics obtained by the two-step sintering method exhibit improved mechanical properties, compared to conventionally sintered BCP (C) 2010 Elsevier Ltd All rights reserved
T2  - Journal of the European Ceramic Society
T1  - Dense fine-grained biphasic calcium phosphate (BCP) bioceramics designed by two-step sintering
VL  - 31
IS  - 1-2
SP  - 19
EP  - 27
DO  - 10.1016/j.jeurceramsoc.2010.09.006
ER  - 
@article{
author = "Lukic, M. and Stojanović, Zoran S. and Škapin, Srečo Davor and Macek-Krzmanc, M. and Mitrić, Miodrag and Markovic, S. and Uskoković, Dragan",
year = "2011",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4155",
abstract = "In this study, dense fine-grained biphasic calcium phosphate bioceramics were designed via the two-step sintering method The starting powder was nanosized calcium-deficient hydroxyapatite, whose phase composition, average particle size and morphology were characterized by XRD, FTIR, Raman spectroscopy, laser diffraction and FE-SEM The phase transformations of the initial powder during heating up to 1200 degrees C were examined using TG/DSC At first, conventional sintering was performed and the recorded shrinkage/densification data were used to find out the appropriate experimental conditions for two step sintering The obtained results show that two step sintering yields BCP ceramics consisting of hydroxyapatite and beta-TCP with lull dense, homogeneous structure with average grain size of 375 nm Furthermore BCP ceramics obtained by the two-step sintering method exhibit improved mechanical properties, compared to conventionally sintered BCP (C) 2010 Elsevier Ltd All rights reserved",
journal = "Journal of the European Ceramic Society",
title = "Dense fine-grained biphasic calcium phosphate (BCP) bioceramics designed by two-step sintering",
volume = "31",
number = "1-2",
pages = "19-27",
doi = "10.1016/j.jeurceramsoc.2010.09.006"
}
Lukic, M., Stojanović, Z. S., Škapin, S. D., Macek-Krzmanc, M., Mitrić, M., Markovic, S.,& Uskoković, D. (2011). Dense fine-grained biphasic calcium phosphate (BCP) bioceramics designed by two-step sintering.
Journal of the European Ceramic Society, 31(1-2), 19-27.
https://doi.org/10.1016/j.jeurceramsoc.2010.09.006
Lukic M, Stojanović ZS, Škapin SD, Macek-Krzmanc M, Mitrić M, Markovic S, Uskoković D. Dense fine-grained biphasic calcium phosphate (BCP) bioceramics designed by two-step sintering. Journal of the European Ceramic Society. 2011;31(1-2):19-27
Lukic M., Stojanović Zoran S., Škapin Srečo Davor, Macek-Krzmanc M., Mitrić Miodrag, Markovic S., Uskoković Dragan, "Dense fine-grained biphasic calcium phosphate (BCP) bioceramics designed by two-step sintering" Journal of the European Ceramic Society, 31, no. 1-2 (2011):19-27,
https://doi.org/10.1016/j.jeurceramsoc.2010.09.006 .
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Controlled assembly of Poly(D,L-lactide-co-glycolide)/hydroxyapatite core-shell nanospheres under ultrasonic irradiation

Jevtic, M.; Radulovic, A.; Ignjatović, Nenad L.; Mitrić, Miodrag; Uskoković, Dragan

(2009)

TY  - JOUR
AU  - Jevtic, M.
AU  - Radulovic, A.
AU  - Ignjatović, Nenad L.
AU  - Mitrić, Miodrag
AU  - Uskoković, Dragan
PY  - 2009
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3597
AB  - An ultrasound field was applied to obtain PLGA/HAp biocomposite nanospheres. Formulation of PLGA/HAp, composite revealed significant dependence of the morphology of the obtained composite on synthesis parameters, like the intensity of applied ultrasonic field, polymeric and ceramic parts wt.% ratio in the composite, temperature of the medium, type of surfactant, and the sequence of steps in the formation of PLGA/HAp. Optimal parameters for the formation of PLGA/HAp included a lower content of the ceramic phase (PLGA/HAp = 90: 10), higher power of ultrasonic field (P = 142.4 W), lower temperature of the medium during ultrasonic treatment (T = 8 degrees C), dilute solution of PVP as surfactant and dispersion of hydroxyapatite in polymer solution in order to achieve required homogeneity before the formulation of the composite. The morphology of PLGA/HAp particles synthesized under these conditions was highly regular: sphere-like, with particles of very small dimensions (150-320 nm), highly uniform particle size distribution and characteristic planar spatial self-organization. These characteristics indicate significant improvements in PLGA/HAp composite resulting from ultrasonic method. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
T2  - Acta Biomaterialia
T1  - Controlled assembly of Poly(D,L-lactide-co-glycolide)/hydroxyapatite core-shell nanospheres under ultrasonic irradiation
VL  - 5
IS  - 1
SP  - 208
EP  - 218
DO  - 10.1016/j.actbio.2008.07.026
ER  - 
@article{
author = "Jevtic, M. and Radulovic, A. and Ignjatović, Nenad L. and Mitrić, Miodrag and Uskoković, Dragan",
year = "2009",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3597",
abstract = "An ultrasound field was applied to obtain PLGA/HAp biocomposite nanospheres. Formulation of PLGA/HAp, composite revealed significant dependence of the morphology of the obtained composite on synthesis parameters, like the intensity of applied ultrasonic field, polymeric and ceramic parts wt.% ratio in the composite, temperature of the medium, type of surfactant, and the sequence of steps in the formation of PLGA/HAp. Optimal parameters for the formation of PLGA/HAp included a lower content of the ceramic phase (PLGA/HAp = 90: 10), higher power of ultrasonic field (P = 142.4 W), lower temperature of the medium during ultrasonic treatment (T = 8 degrees C), dilute solution of PVP as surfactant and dispersion of hydroxyapatite in polymer solution in order to achieve required homogeneity before the formulation of the composite. The morphology of PLGA/HAp particles synthesized under these conditions was highly regular: sphere-like, with particles of very small dimensions (150-320 nm), highly uniform particle size distribution and characteristic planar spatial self-organization. These characteristics indicate significant improvements in PLGA/HAp composite resulting from ultrasonic method. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.",
journal = "Acta Biomaterialia",
title = "Controlled assembly of Poly(D,L-lactide-co-glycolide)/hydroxyapatite core-shell nanospheres under ultrasonic irradiation",
volume = "5",
number = "1",
pages = "208-218",
doi = "10.1016/j.actbio.2008.07.026"
}
Jevtic, M., Radulovic, A., Ignjatović, N. L., Mitrić, M.,& Uskoković, D. (2009). Controlled assembly of Poly(D,L-lactide-co-glycolide)/hydroxyapatite core-shell nanospheres under ultrasonic irradiation.
Acta Biomaterialia, 5(1), 208-218.
https://doi.org/10.1016/j.actbio.2008.07.026
Jevtic M, Radulovic A, Ignjatović NL, Mitrić M, Uskoković D. Controlled assembly of Poly(D,L-lactide-co-glycolide)/hydroxyapatite core-shell nanospheres under ultrasonic irradiation. Acta Biomaterialia. 2009;5(1):208-218
Jevtic M., Radulovic A., Ignjatović Nenad L., Mitrić Miodrag, Uskoković Dragan, "Controlled assembly of Poly(D,L-lactide-co-glycolide)/hydroxyapatite core-shell nanospheres under ultrasonic irradiation" Acta Biomaterialia, 5, no. 1 (2009):208-218,
https://doi.org/10.1016/j.actbio.2008.07.026 .
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An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres

Stevanovic, Magdalena; Maksin, Tatjana N.; Petkovic, Jana; Filipic, Metka; Uskoković, Dragan

(2009)

TY  - JOUR
AU  - Stevanovic, Magdalena
AU  - Maksin, Tatjana N.
AU  - Petkovic, Jana
AU  - Filipic, Metka
AU  - Uskoković, Dragan
PY  - 2009
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3755
AB  - Nanoparticles of poly(DL-lactide-co-glycolide) (PLGA) in the size range 90-150 nm were produced using the physicochemical method with solvent/non-solvent systems. The encapsulation of the ascorbic acid in the polymer matrix was performed by homogenization of the water and organic phases. In vitro degradation and release tests of PLGA nanoparticles with and without encapsulated ascorbic acid were studied for more than 60 days in PBS and it has been determined that PLGA completely degrades within this period, fully releasing all encapsulated ascorbic acid. The cytotoxicity of PLGA and PLGA/ascorbic acid 85/15% nanoparticles was examined with human hepatoma cell lines (HepG2 ECACC), in vitro. The obtained results indicate that neither PLGA nanospheres nor PLGA/ascorbic acid 85/15% nanoparticles significantly affected the viability of the HepG2 cells. The investigation of the distribution and pharmacokinetics of PLGA is crucial for the effective prediction of host responses to PLGA in particular applications. Thus we present a method of labeling PLGA nanospheres and PLGA/ascorbic acid 85/15 wt% nanoparticles by (99m)Tc which binds outside, leaving the cage intact. This enables a quick and convenient investigation of the pharmacological behavior and metabolism of PLGA. The biodistribution of (99m)Tc-labeled PLGA particles with and without encapsulated ascorbic acid after different periods of time of their installation into rats was examined. PLGA nanospheres with encapsulated ascorbic acid exhibit prolonged blood circulation accompanied by time-dependent reduction in the lungs, liver and spleen, and addition in the kidney, stomach and intestine. The samples were characterized by x-ray diffraction, scanning electron microscopy, stereological analysis, transmission electron microscopy, ultraviolet spectroscopy and instant thin layer chromatography.
T2  - Nanotechnology
T1  - An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres
VL  - 20
IS  - 33
DO  - 10.1088/0957-4484/20/33/335102
ER  - 
@article{
author = "Stevanovic, Magdalena and Maksin, Tatjana N. and Petkovic, Jana and Filipic, Metka and Uskoković, Dragan",
year = "2009",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3755",
abstract = "Nanoparticles of poly(DL-lactide-co-glycolide) (PLGA) in the size range 90-150 nm were produced using the physicochemical method with solvent/non-solvent systems. The encapsulation of the ascorbic acid in the polymer matrix was performed by homogenization of the water and organic phases. In vitro degradation and release tests of PLGA nanoparticles with and without encapsulated ascorbic acid were studied for more than 60 days in PBS and it has been determined that PLGA completely degrades within this period, fully releasing all encapsulated ascorbic acid. The cytotoxicity of PLGA and PLGA/ascorbic acid 85/15% nanoparticles was examined with human hepatoma cell lines (HepG2 ECACC), in vitro. The obtained results indicate that neither PLGA nanospheres nor PLGA/ascorbic acid 85/15% nanoparticles significantly affected the viability of the HepG2 cells. The investigation of the distribution and pharmacokinetics of PLGA is crucial for the effective prediction of host responses to PLGA in particular applications. Thus we present a method of labeling PLGA nanospheres and PLGA/ascorbic acid 85/15 wt% nanoparticles by (99m)Tc which binds outside, leaving the cage intact. This enables a quick and convenient investigation of the pharmacological behavior and metabolism of PLGA. The biodistribution of (99m)Tc-labeled PLGA particles with and without encapsulated ascorbic acid after different periods of time of their installation into rats was examined. PLGA nanospheres with encapsulated ascorbic acid exhibit prolonged blood circulation accompanied by time-dependent reduction in the lungs, liver and spleen, and addition in the kidney, stomach and intestine. The samples were characterized by x-ray diffraction, scanning electron microscopy, stereological analysis, transmission electron microscopy, ultraviolet spectroscopy and instant thin layer chromatography.",
journal = "Nanotechnology",
title = "An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres",
volume = "20",
number = "33",
doi = "10.1088/0957-4484/20/33/335102"
}
Stevanovic, M., Maksin, T. N., Petkovic, J., Filipic, M.,& Uskoković, D. (2009). An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres.
Nanotechnology, 20(33).
https://doi.org/10.1088/0957-4484/20/33/335102
Stevanovic M, Maksin TN, Petkovic J, Filipic M, Uskoković D. An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres. Nanotechnology. 2009;20(33)
Stevanovic Magdalena, Maksin Tatjana N., Petkovic Jana, Filipic Metka, Uskoković Dragan, "An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres" Nanotechnology, 20, no. 33 (2009),
https://doi.org/10.1088/0957-4484/20/33/335102 .
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