TY  - JOUR
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Kasapović, Jelena
AU  - Pajović, Snežana B.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3877
AB  - Background: Oxidative stress and impaired antioxidant system have been proposed as a potential factors involved in the pathophysiology of diverse disease states, including carcinogenesis. In this study, we explored the lipid peroxidation levels and antioxidant enzyme activities in women diagnosed with different forms of gynecological diseases in order to evaluate the antioxidant status in endometrium of such patients. Methods: Endometrial tissues of gynecological patients with different diagnoses were collected and subjected to assays for superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and lipid hydroperoxides. Results: Superoxide dismutase activity was significantly decreased (50% in average) in hyperplastic and adenocarcinoma patients. Activities of both glutathione peroxidase and glutathione reductase were increased 60% and 100% on average, in hyperplastic patients, while in adenocarcinoma patients only glutathione reductase activity was elevated 100%. Catalase activity was significantly decreased in adenocarcinoma patients (47%). Lipid hydroperoxides level was negatively correlated to superoxide dismutase and catalase activities, and positively correlated to glutathione peroxidase and glutathione reductase activities. Conclusions: This study provided the first comparison of antioxidant status and lipid peroxidation in endometrial tissues of patients with polyps, myoma, hyperplasia and adenocarcinoma. The results showed that patients with premalignant (hyperplastic) and malignant (adenocarcinoma) lesions had enhanced lipid peroxidation and altered uterine antioxidant enzyme activities than patients with benign uterine diseases, polyps and myoma, although the extent of disturbance varied with the diagnosis. Further investigation is needed to clarify the mechanisms responsible for the observed alterations and whether lipid hydroperoxide levels and antioxidant enzyme activities in uterus of gynecological patients might be used as additional parameter in clinical evaluation of gynecological disorders.
T2  - Reproductive Biology and Endocrinology
T1  - Antioxidant enzymes and lipid peroxidation in endometrium of patients with polyps, myoma, hyperplasia and adenocarcinoma
VL  - 7
DO  - 10.1186/1477-7827-7-149
ER  - 

@article{
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Kasapović, Jelena and Pajović, Snežana B.",
year = "2009",
abstract = "Background: Oxidative stress and impaired antioxidant system have been proposed as a potential factors involved in the pathophysiology of diverse disease states, including carcinogenesis. In this study, we explored the lipid peroxidation levels and antioxidant enzyme activities in women diagnosed with different forms of gynecological diseases in order to evaluate the antioxidant status in endometrium of such patients. Methods: Endometrial tissues of gynecological patients with different diagnoses were collected and subjected to assays for superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and lipid hydroperoxides. Results: Superoxide dismutase activity was significantly decreased (50% in average) in hyperplastic and adenocarcinoma patients. Activities of both glutathione peroxidase and glutathione reductase were increased 60% and 100% on average, in hyperplastic patients, while in adenocarcinoma patients only glutathione reductase activity was elevated 100%. Catalase activity was significantly decreased in adenocarcinoma patients (47%). Lipid hydroperoxides level was negatively correlated to superoxide dismutase and catalase activities, and positively correlated to glutathione peroxidase and glutathione reductase activities. Conclusions: This study provided the first comparison of antioxidant status and lipid peroxidation in endometrial tissues of patients with polyps, myoma, hyperplasia and adenocarcinoma. The results showed that patients with premalignant (hyperplastic) and malignant (adenocarcinoma) lesions had enhanced lipid peroxidation and altered uterine antioxidant enzyme activities than patients with benign uterine diseases, polyps and myoma, although the extent of disturbance varied with the diagnosis. Further investigation is needed to clarify the mechanisms responsible for the observed alterations and whether lipid hydroperoxide levels and antioxidant enzyme activities in uterus of gynecological patients might be used as additional parameter in clinical evaluation of gynecological disorders.",
journal = "Reproductive Biology and Endocrinology",
title = "Antioxidant enzymes and lipid peroxidation in endometrium of patients with polyps, myoma, hyperplasia and adenocarcinoma",
volume = "7",
doi = "10.1186/1477-7827-7-149"
}

Pejić, S., Todorović, A., Stojiljković, V., Kasapović, J.,& Pajović, S. B.. (2009). Antioxidant enzymes and lipid peroxidation in endometrium of patients with polyps, myoma, hyperplasia and adenocarcinoma. in Reproductive Biology and Endocrinology, 7.
https://doi.org/10.1186/1477-7827-7-149

Pejić S, Todorović A, Stojiljković V, Kasapović J, Pajović SB. Antioxidant enzymes and lipid peroxidation in endometrium of patients with polyps, myoma, hyperplasia and adenocarcinoma. in Reproductive Biology and Endocrinology. 2009;7.
doi:10.1186/1477-7827-7-149 .

Pejić, Snežana, Todorović, Ana, Stojiljković, Vesna, Kasapović, Jelena, Pajović, Snežana B., "Antioxidant enzymes and lipid peroxidation in endometrium of patients with polyps, myoma, hyperplasia and adenocarcinoma" in Reproductive Biology and Endocrinology, 7 (2009),
https://doi.org/10.1186/1477-7827-7-149 . .

TY  - JOUR
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Cvetković, Dragana
AU  - Lucic, Nenad
AU  - Radojicic, Ratko M.
AU  - Saičić, Zorica S.
AU  - Pajović, Snežana B.
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3520
AB  - Epidemiological and experimental data point to involvement of oxygen derived radicals in the pathogenesis of gynecological disorders, as well as in cancer development. The objective of the present study was to examine changes ill activities and levels of copper/zinc superoxide dismutase (CuZnSOD) and lipid hydroperoxides (LOOH) in blood and endometrial tissue of patients diagnosed With uterine myoma, endometrial polypus, hyperplasia simplex, hyperplasia complex and adenocarcinoma endometrii. The results of our study have shown decreased SOD activities and unchanged SOD protein level in blood of all examined patients in comparison to healthy Subjects. Decrease of both SOD activity and level was Found in endometrium of patients with hyperplasia simplex, hyperplasia complex and adenocarcinoma in comparison to women with polypus or myoma. LOOH level was elevated in both tissues of patients with hyperplasia or adenocarcinoma in comparison to healthy Subjects or patients with benign diagnosis. Our findings suggest that the decrease in SOD activity and level, as well as the increase in LOOH level, in patients with gynecological disorders. render these patients more Susceptible to oxidative damage caused by reactive oxygen species (ROS). An imbalance in ROS formation and SOD level may be important in the pathogenesis and/or perpetuation of tissue damage in gynecological patients. Since evidence suggests that SOD may be a therapy target for cancer treatment, our findings provide a basis for further research and options for clinical applications.
T2  - Anais de Academia Brasileira de Ciencias
T1  - Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium
VL  - 80
IS  - 3
SP  - 515
EP  - 522
DO  - 10.1590/S0001-37652008000300011
ER  - 

@article{
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Cvetković, Dragana and Lucic, Nenad and Radojicic, Ratko M. and Saičić, Zorica S. and Pajović, Snežana B.",
year = "2008",
abstract = "Epidemiological and experimental data point to involvement of oxygen derived radicals in the pathogenesis of gynecological disorders, as well as in cancer development. The objective of the present study was to examine changes ill activities and levels of copper/zinc superoxide dismutase (CuZnSOD) and lipid hydroperoxides (LOOH) in blood and endometrial tissue of patients diagnosed With uterine myoma, endometrial polypus, hyperplasia simplex, hyperplasia complex and adenocarcinoma endometrii. The results of our study have shown decreased SOD activities and unchanged SOD protein level in blood of all examined patients in comparison to healthy Subjects. Decrease of both SOD activity and level was Found in endometrium of patients with hyperplasia simplex, hyperplasia complex and adenocarcinoma in comparison to women with polypus or myoma. LOOH level was elevated in both tissues of patients with hyperplasia or adenocarcinoma in comparison to healthy Subjects or patients with benign diagnosis. Our findings suggest that the decrease in SOD activity and level, as well as the increase in LOOH level, in patients with gynecological disorders. render these patients more Susceptible to oxidative damage caused by reactive oxygen species (ROS). An imbalance in ROS formation and SOD level may be important in the pathogenesis and/or perpetuation of tissue damage in gynecological patients. Since evidence suggests that SOD may be a therapy target for cancer treatment, our findings provide a basis for further research and options for clinical applications.",
journal = "Anais de Academia Brasileira de Ciencias",
title = "Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium",
volume = "80",
number = "3",
pages = "515-522",
doi = "10.1590/S0001-37652008000300011"
}

Pejić, S., Todorović, A., Stojiljković, V., Cvetković, D., Lucic, N., Radojicic, R. M., Saičić, Z. S.,& Pajović, S. B.. (2008). Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium. in Anais de Academia Brasileira de Ciencias, 80(3), 515-522.
https://doi.org/10.1590/S0001-37652008000300011

Pejić S, Todorović A, Stojiljković V, Cvetković D, Lucic N, Radojicic RM, Saičić ZS, Pajović SB. Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium. in Anais de Academia Brasileira de Ciencias. 2008;80(3):515-522.
doi:10.1590/S0001-37652008000300011 .

Pejić, Snežana, Todorović, Ana, Stojiljković, Vesna, Cvetković, Dragana, Lucic, Nenad, Radojicic, Ratko M., Saičić, Zorica S., Pajović, Snežana B., "Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium" in Anais de Academia Brasileira de Ciencias, 80, no. 3 (2008):515-522,
https://doi.org/10.1590/S0001-37652008000300011 . .