TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9617
AB  - This study aimed to investigate the effects of chronic restraint stress (CRS) on the protein levels of dopamine-β-hydroxylase (DBH), noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and brain-derived neurotrophic factor (BDNF), as well as the concentration of noradrenaline (NA) in the rat hippocampus. The investigated parameters were quantified by Western blot analyses and ELISA kits. We found that CRS increased the protein levels of DBH by 30 %, VMAT2 by 11 %, BDNF by 11 % and the concentration of NA by 104 %, but decreased the protein levels of NET by 16 % in the hippocampus of chronically stressed rats. The molecular mechanisms by which CRS increased the hippocampal NA level are an important adaptive phenomenon of the noradrenergic system in the stress condition. © 2020 Charles University. All rights reserved.
T2  - Folia Biologica (Czech Republic)
T1  - Changes of Hippocampal Noradrenergic Capacity in Stress Condition
VL  - 66
IS  - 2
SP  - 81
EP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9617
ER  - 

@article{
author = "Gavrilović, Ljubica and Popović, Nataša M. and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Pajović, Snežana B.",
year = "2020",
abstract = "This study aimed to investigate the effects of chronic restraint stress (CRS) on the protein levels of dopamine-β-hydroxylase (DBH), noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and brain-derived neurotrophic factor (BDNF), as well as the concentration of noradrenaline (NA) in the rat hippocampus. The investigated parameters were quantified by Western blot analyses and ELISA kits. We found that CRS increased the protein levels of DBH by 30 %, VMAT2 by 11 %, BDNF by 11 % and the concentration of NA by 104 %, but decreased the protein levels of NET by 16 % in the hippocampus of chronically stressed rats. The molecular mechanisms by which CRS increased the hippocampal NA level are an important adaptive phenomenon of the noradrenergic system in the stress condition. © 2020 Charles University. All rights reserved.",
journal = "Folia Biologica (Czech Republic)",
title = "Changes of Hippocampal Noradrenergic Capacity in Stress Condition",
volume = "66",
number = "2",
pages = "81-84",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9617"
}

Gavrilović, L., Popović, N. M., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I.,& Pajović, S. B.. (2020). Changes of Hippocampal Noradrenergic Capacity in Stress Condition. in Folia Biologica (Czech Republic), 66(2), 81-84.
https://hdl.handle.net/21.15107/rcub_vinar_9617

Gavrilović L, Popović NM, Stojiljković V, Pejić S, Todorović A, Pavlović I, Pajović SB. Changes of Hippocampal Noradrenergic Capacity in Stress Condition. in Folia Biologica (Czech Republic). 2020;66(2):81-84.
https://hdl.handle.net/21.15107/rcub_vinar_9617 .

Gavrilović, Ljubica, Popović, Nataša M., Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Pajović, Snežana B., "Changes of Hippocampal Noradrenergic Capacity in Stress Condition" in Folia Biologica (Czech Republic), 66, no. 2 (2020):81-84,
https://hdl.handle.net/21.15107/rcub_vinar_9617 .

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Stojiljković, Vesna
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
AU  - Pajović, Snežana B.
PY  - 2019
UR  - https://www.hindawi.com/journals/omcl/2019/8745376/
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8085
AB  - This study examined the effects of lithium on gene expression and activity of the antioxidant enzymes copper zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the hippocampus of chronically stressed rats. In addition, we examined the effects of lithium on anxiety behaviors, hippocampal concentrations of dopamine (DA) and malondialdehyde (MDA), protein levels of brain-derived neurotrophic factor (BDNF), tyrosine hydroxylase (TH), dopamine transporter (DAT), and catechol-O-methyltransferase (COMT), as well as activity of monoamine oxidase (MAO) in chronically stressed rats. The investigated parameters were quantified by real-time RT-PCR, Western blot analyses, and assays of enzyme activities. We found that lithium did not change gene expression of SOD1, CAT, GPx, and GR but decreased gene expression of SOD2 in chronically stressed rats. A very important result in this study was that lithium treatment decreased the enzyme activities of SOD1 and SOD2 but increased the enzyme activities of GPx and GR in stress condition, which indicates the control of redox balance. The reduced concentration of MDA confirms this. In addition, we found that lithium treatment decreased high protein levels of BDNF and DAT in chronically stressed rats to the level found in unstressed animals. Also, lithium treatment increased the expression of TH but decreased the enzyme activity of MAO B, which contributed to the increase of hippocampal concentration of DA in chronically stressed rats to the level of unstressed animals. Finally, lithium treatment in animals exposed to chronic stress increased the time spent in open arms. Lithium-induced modulation of hippocampal antioxidant status and attenuation of oxidative stress stabilized behavior in animals with high anxiety index. In addition, reduced oxidative stress was followed by the changes of both turnover of DA and levels of BDNF protein in chronically stressed rats treated with lithium. These findings may be important in preclinical research of the effects of lithium on oxidative stress level in pathological conditions.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium
VL  - 2019
SP  - 1
EP  - 11
DO  - 10.1155/2019/8745376
ER  - 

@article{
author = "Popović, Nataša M. and Stojiljković, Vesna and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Gavrilović, Ljubica and Pajović, Snežana B.",
year = "2019",
abstract = "This study examined the effects of lithium on gene expression and activity of the antioxidant enzymes copper zinc superoxide dismutase (SOD1), manganese superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the hippocampus of chronically stressed rats. In addition, we examined the effects of lithium on anxiety behaviors, hippocampal concentrations of dopamine (DA) and malondialdehyde (MDA), protein levels of brain-derived neurotrophic factor (BDNF), tyrosine hydroxylase (TH), dopamine transporter (DAT), and catechol-O-methyltransferase (COMT), as well as activity of monoamine oxidase (MAO) in chronically stressed rats. The investigated parameters were quantified by real-time RT-PCR, Western blot analyses, and assays of enzyme activities. We found that lithium did not change gene expression of SOD1, CAT, GPx, and GR but decreased gene expression of SOD2 in chronically stressed rats. A very important result in this study was that lithium treatment decreased the enzyme activities of SOD1 and SOD2 but increased the enzyme activities of GPx and GR in stress condition, which indicates the control of redox balance. The reduced concentration of MDA confirms this. In addition, we found that lithium treatment decreased high protein levels of BDNF and DAT in chronically stressed rats to the level found in unstressed animals. Also, lithium treatment increased the expression of TH but decreased the enzyme activity of MAO B, which contributed to the increase of hippocampal concentration of DA in chronically stressed rats to the level of unstressed animals. Finally, lithium treatment in animals exposed to chronic stress increased the time spent in open arms. Lithium-induced modulation of hippocampal antioxidant status and attenuation of oxidative stress stabilized behavior in animals with high anxiety index. In addition, reduced oxidative stress was followed by the changes of both turnover of DA and levels of BDNF protein in chronically stressed rats treated with lithium. These findings may be important in preclinical research of the effects of lithium on oxidative stress level in pathological conditions.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium",
volume = "2019",
pages = "1-11",
doi = "10.1155/2019/8745376"
}

Popović, N. M., Stojiljković, V., Pejić, S., Todorović, A., Pavlović, I., Gavrilović, L.,& Pajović, S. B.. (2019). Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium. in Oxidative Medicine and Cellular Longevity, 2019, 1-11.
https://doi.org/10.1155/2019/8745376

Popović NM, Stojiljković V, Pejić S, Todorović A, Pavlović I, Gavrilović L, Pajović SB. Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium. in Oxidative Medicine and Cellular Longevity. 2019;2019:1-11.
doi:10.1155/2019/8745376 .

Popović, Nataša M., Stojiljković, Vesna, Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Gavrilović, Ljubica, Pajović, Snežana B., "Modulation of Hippocampal Antioxidant Defense System in Chronically Stressed Rats by Lithium" in Oxidative Medicine and Cellular Longevity, 2019 (2019):1-11,
https://doi.org/10.1155/2019/8745376 . .

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
AU  - Stojiljković, Vesna
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8404
AB  - The effects of chronic restraint stress (CRS, 2 h during 14 days) on gene expression of tyrosine hydroxylase (TH), catechol-O-methyltransferase (COMT), and glutathione peroxidase (GPx) were studied in the rat hippocampus. Changes in the dopamine (DA) concentration and activities of monoamine oxidases (MAO A and MAO B) and GPx in this cerebral structure of chronically stressed rats were also examined. The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assay of enzymatic activity. We found that CRS decreased the TH protein level and DA concentration, which probably confirms the statement that de novo synthesis of DA is suppressed under stress conditions. The increased activities of MAO B, as well as the increased level of COMT protein, are believed to be related to intensified DA catabolism conditions. Also, a decreased activity of GPx in the hippocampus of chronically stressed animals was found. The increased enzymatic activity of MAO B negatively correlated with the reduced activity of GPx under the above-mentioned stress conditions. These events in the hippocampus of chronically stressed animals could synergistically cause oxidative damage to the mitochondria. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Neurophysiology
T1  - Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats
VL  - 50
IS  - 5
SP  - 332
EP  - 338
DO  - 10.1007/s11062-019-09758-z
ER  - 

@article{
author = "Popović, Nataša M. and Pajović, Snežana B. and Stojiljković, Vesna and Todorović, Ana and Pejić, Snežana and Pavlović, Ivan and Gavrilović, Ljubica",
year = "2018",
abstract = "The effects of chronic restraint stress (CRS, 2 h during 14 days) on gene expression of tyrosine hydroxylase (TH), catechol-O-methyltransferase (COMT), and glutathione peroxidase (GPx) were studied in the rat hippocampus. Changes in the dopamine (DA) concentration and activities of monoamine oxidases (MAO A and MAO B) and GPx in this cerebral structure of chronically stressed rats were also examined. The investigated parameters were quantified using real-time RT-PCR, Western blot analyses, and assay of enzymatic activity. We found that CRS decreased the TH protein level and DA concentration, which probably confirms the statement that de novo synthesis of DA is suppressed under stress conditions. The increased activities of MAO B, as well as the increased level of COMT protein, are believed to be related to intensified DA catabolism conditions. Also, a decreased activity of GPx in the hippocampus of chronically stressed animals was found. The increased enzymatic activity of MAO B negatively correlated with the reduced activity of GPx under the above-mentioned stress conditions. These events in the hippocampus of chronically stressed animals could synergistically cause oxidative damage to the mitochondria. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Neurophysiology",
title = "Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats",
volume = "50",
number = "5",
pages = "332-338",
doi = "10.1007/s11062-019-09758-z"
}

Popović, N. M., Pajović, S. B., Stojiljković, V., Todorović, A., Pejić, S., Pavlović, I.,& Gavrilović, L.. (2018). Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats. in Neurophysiology, 50(5), 332-338.
https://doi.org/10.1007/s11062-019-09758-z

Popović NM, Pajović SB, Stojiljković V, Todorović A, Pejić S, Pavlović I, Gavrilović L. Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats. in Neurophysiology. 2018;50(5):332-338.
doi:10.1007/s11062-019-09758-z .

Popović, Nataša M., Pajović, Snežana B., Stojiljković, Vesna, Todorović, Ana, Pejić, Snežana, Pavlović, Ivan, Gavrilović, Ljubica, "Activities of the Dopaminergic System and Glutathione Antioxidant System in the Hippocampus of Stressed rats" in Neurophysiology, 50, no. 5 (2018):332-338,
https://doi.org/10.1007/s11062-019-09758-z . .

TY  - CHAP
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Popović, Nataša M.
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8406
AB  - We examined the effects of daily exercise on the gene expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), and phenyl
ethanolamine N-methyltransferase (PNMT)), vesicular monoamine transporter 2 (VMAT 2),
antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)), concentrations of catecholamines (noradrenaline (NA) and adrenaline (A))
and malondialdehyde (MDA), activities of monoamine oxidase (MAO), and antioxidant
enzymes in the spleen of chronically psychosocially stressed rats. Exposure of chronically
stressed rats to exercise increased the levels of PNMT protein by 19%, VMAT 2 mRNA by
100%, NA by 160%, and A by 140%; decreased/unchanged MAO enzyme activity; returned
concentrations of MDA to control level; and increased CAT and GPx mRNA levels (50%
and 150%, respectively). Exercise induced the accumulation of the catecholamines and a
decrease of stress-induced oxidative stress in the spleen, which may significantly affect the
immune-neuroendocrine interactions in stress conditions. Also, exercise induced the catecholaminergic system and antioxidant defense to become more ready to a novel stressor,
which indicates that exercise may induce potentially positive physiological adaptations.
Our combined model of chronic social isolation and long-term daily treadmill running in
rats may be a good animal model in the research of therapeutic role of exercise in human
disease caused by chronic stress.
PB  - IntechOpen
PB  - InTech
T2  - Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh
T1  - Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System
IS  - Ch. 14
SP  - 283
EP  - 310
DO  - 10.5772/intechopen.70008
ER  - 

@inbook{
editor = "Bartholomew, Ibeh",
author = "Gavrilović, Ljubica and Stojiljković, Vesna and Popović, Nataša M. and Pejić, Snežana and Todorović, Ana and Pavlović, Ivan and Pajović, Snežana B.",
year = "2018",
abstract = "We examined the effects of daily exercise on the gene expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-β-hydroxylase (DBH), and phenyl
ethanolamine N-methyltransferase (PNMT)), vesicular monoamine transporter 2 (VMAT 2),
antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)), concentrations of catecholamines (noradrenaline (NA) and adrenaline (A))
and malondialdehyde (MDA), activities of monoamine oxidase (MAO), and antioxidant
enzymes in the spleen of chronically psychosocially stressed rats. Exposure of chronically
stressed rats to exercise increased the levels of PNMT protein by 19%, VMAT 2 mRNA by
100%, NA by 160%, and A by 140%; decreased/unchanged MAO enzyme activity; returned
concentrations of MDA to control level; and increased CAT and GPx mRNA levels (50%
and 150%, respectively). Exercise induced the accumulation of the catecholamines and a
decrease of stress-induced oxidative stress in the spleen, which may significantly affect the
immune-neuroendocrine interactions in stress conditions. Also, exercise induced the catecholaminergic system and antioxidant defense to become more ready to a novel stressor,
which indicates that exercise may induce potentially positive physiological adaptations.
Our combined model of chronic social isolation and long-term daily treadmill running in
rats may be a good animal model in the research of therapeutic role of exercise in human
disease caused by chronic stress.",
publisher = "IntechOpen, InTech",
journal = "Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh",
booktitle = "Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System",
number = "Ch. 14",
pages = "283-310",
doi = "10.5772/intechopen.70008"
}

Bartholomew, I., Gavrilović, L., Stojiljković, V., Popović, N. M., Pejić, S., Todorović, A., Pavlović, I.,& Pajović, S. B.. (2018). Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System. in Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh
IntechOpen.(Ch. 14), 283-310.
https://doi.org/10.5772/intechopen.70008

Bartholomew I, Gavrilović L, Stojiljković V, Popović NM, Pejić S, Todorović A, Pavlović I, Pajović SB. Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System. in Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh. 2018;(Ch. 14):283-310.
doi:10.5772/intechopen.70008 .

Bartholomew, Ibeh, Gavrilović, Ljubica, Stojiljković, Vesna, Popović, Nataša M., Pejić, Snežana, Todorović, Ana, Pavlović, Ivan, Pajović, Snežana B., "Animal Models for Chronic Stress-Induced Oxidative Stress in the Spleen: The Role of Exercise and Catecholaminergic System" in Experimental Animal Models of Human Diseases : An Effective Therapeutic Strategy, (ed.) Bartholomew Ibeh, no. Ch. 14 (2018):283-310,
https://doi.org/10.5772/intechopen.70008 . .

TY  - JOUR
AU  - Đurić, Biljana
AU  - Suzić, Slavica
AU  - Stojadinović, Bojana
AU  - Nestorović, Zorica
AU  - Ivanović, Marija D.
AU  - Suzić-Lazić, Jelena
AU  - Nešić, Dejan
AU  - Mazić, Sanja
AU  - Tenne, Tamar
AU  - Zikich, Dragoslav
AU  - Žikić, Dejan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1655
AB  - We present here the improved design and development of optical sensor for non-invasive measurements of arterial blood flow waveform. The sensor is based on a physical principle of reflective photoplethysmography (PPG). As the light source we used serially connected infrared diodes whereas NPN silicon phototransistors were used as light detectors. The electronic components were molded into square package and poured with silicone. Such preparation produced an elastic superficies that allowed excellent attachment of the sensor on the skins surface. Moreover, a serial connection of infrared diodes and phototransistors completely eliminated signal artifacts caused by minor muscle contractions. The sensor recording performances were examined at the photoplethysmographic sites on three different arteries; the commune carotid, femoral and radial and, on each site the sensor demonstrated remarkable capability to make a consistent, reproducible measurements. Because of the advantageous physical and electrical properties, the new sensor is suitable for various cardiovascular diagnostics procedures, especially when long-term measurements of arterial blood flow waveform are required, for monitoring of different parameters in cardiovascular units and for research.
T2  - Biomedical Microdevices
T1  - An improved design of optical sensor for long-term measurement of arterial blood flow waveform
VL  - 19
IS  - 3
DO  - 10.1007/s10544-017-0196-x
ER  - 

@article{
author = "Đurić, Biljana and Suzić, Slavica and Stojadinović, Bojana and Nestorović, Zorica and Ivanović, Marija D. and Suzić-Lazić, Jelena and Nešić, Dejan and Mazić, Sanja and Tenne, Tamar and Zikich, Dragoslav and Žikić, Dejan",
year = "2017",
abstract = "We present here the improved design and development of optical sensor for non-invasive measurements of arterial blood flow waveform. The sensor is based on a physical principle of reflective photoplethysmography (PPG). As the light source we used serially connected infrared diodes whereas NPN silicon phototransistors were used as light detectors. The electronic components were molded into square package and poured with silicone. Such preparation produced an elastic superficies that allowed excellent attachment of the sensor on the skins surface. Moreover, a serial connection of infrared diodes and phototransistors completely eliminated signal artifacts caused by minor muscle contractions. The sensor recording performances were examined at the photoplethysmographic sites on three different arteries; the commune carotid, femoral and radial and, on each site the sensor demonstrated remarkable capability to make a consistent, reproducible measurements. Because of the advantageous physical and electrical properties, the new sensor is suitable for various cardiovascular diagnostics procedures, especially when long-term measurements of arterial blood flow waveform are required, for monitoring of different parameters in cardiovascular units and for research.",
journal = "Biomedical Microdevices",
title = "An improved design of optical sensor for long-term measurement of arterial blood flow waveform",
volume = "19",
number = "3",
doi = "10.1007/s10544-017-0196-x"
}

Đurić, B., Suzić, S., Stojadinović, B., Nestorović, Z., Ivanović, M. D., Suzić-Lazić, J., Nešić, D., Mazić, S., Tenne, T., Zikich, D.,& Žikić, D.. (2017). An improved design of optical sensor for long-term measurement of arterial blood flow waveform. in Biomedical Microdevices, 19(3).
https://doi.org/10.1007/s10544-017-0196-x

Đurić B, Suzić S, Stojadinović B, Nestorović Z, Ivanović MD, Suzić-Lazić J, Nešić D, Mazić S, Tenne T, Zikich D, Žikić D. An improved design of optical sensor for long-term measurement of arterial blood flow waveform. in Biomedical Microdevices. 2017;19(3).
doi:10.1007/s10544-017-0196-x .

Đurić, Biljana, Suzić, Slavica, Stojadinović, Bojana, Nestorović, Zorica, Ivanović, Marija D., Suzić-Lazić, Jelena, Nešić, Dejan, Mazić, Sanja, Tenne, Tamar, Zikich, Dragoslav, Žikić, Dejan, "An improved design of optical sensor for long-term measurement of arterial blood flow waveform" in Biomedical Microdevices, 19, no. 3 (2017),
https://doi.org/10.1007/s10544-017-0196-x . .

TY  - JOUR
AU  - Pejić, Snežana
AU  - Stojiljković, Vesna
AU  - Todorović, Ana
AU  - Gavrilović, Ljubica
AU  - Pavlović, Ivan
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1301
AB  - The study deals with manganese superoxide dismutase, copper, zinc superoxide dismutase, and catalase activities in brain cortex of Wistar rats exposed to acute stress (immobilization or cold for 2 h), chronic stress (long-term isolation or long-term forced swimming for 21 days), or to combined chronic/acute stress. We observed that i) single episodes of acute stress by immobilization increased activity of both superoxide dismutases; ii) both types of chronic stresses significantly elevated activities of all examined enzymes; iii) chronic social isolation was a much stronger stressor than physical stress by swimming; iv) in animals pre-exposed to chronic isolation, additional stress by immobilization or cold significantly decreased previously elevated activities of all enzymes, while after chronic swimming, acute immobilization lowered only catalase activity. The obtained results indicate that stress conditions most probably altered the cell redox equilibrium, thus influencing the antioxidant response in brain cortex. Further investigation of neuronal prooxidant/antioxidant cellular conditions is needed to improve the prevention and treatment of various stress induced diseases.
T2  - Folia Biologica (Krakow)
T1  - Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress
VL  - 64
IS  - 3
SP  - 189
EP  - 195
DO  - 10.3409/fb64_3.189
ER  - 

@article{
author = "Pejić, Snežana and Stojiljković, Vesna and Todorović, Ana and Gavrilović, Ljubica and Pavlović, Ivan and Popović, Nataša M. and Pajović, Snežana B.",
year = "2016",
abstract = "The study deals with manganese superoxide dismutase, copper, zinc superoxide dismutase, and catalase activities in brain cortex of Wistar rats exposed to acute stress (immobilization or cold for 2 h), chronic stress (long-term isolation or long-term forced swimming for 21 days), or to combined chronic/acute stress. We observed that i) single episodes of acute stress by immobilization increased activity of both superoxide dismutases; ii) both types of chronic stresses significantly elevated activities of all examined enzymes; iii) chronic social isolation was a much stronger stressor than physical stress by swimming; iv) in animals pre-exposed to chronic isolation, additional stress by immobilization or cold significantly decreased previously elevated activities of all enzymes, while after chronic swimming, acute immobilization lowered only catalase activity. The obtained results indicate that stress conditions most probably altered the cell redox equilibrium, thus influencing the antioxidant response in brain cortex. Further investigation of neuronal prooxidant/antioxidant cellular conditions is needed to improve the prevention and treatment of various stress induced diseases.",
journal = "Folia Biologica (Krakow)",
title = "Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress",
volume = "64",
number = "3",
pages = "189-195",
doi = "10.3409/fb64_3.189"
}

Pejić, S., Stojiljković, V., Todorović, A., Gavrilović, L., Pavlović, I., Popović, N. M.,& Pajović, S. B.. (2016). Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress. in Folia Biologica (Krakow), 64(3), 189-195.
https://doi.org/10.3409/fb64_3.189

Pejić S, Stojiljković V, Todorović A, Gavrilović L, Pavlović I, Popović NM, Pajović SB. Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress. in Folia Biologica (Krakow). 2016;64(3):189-195.
doi:10.3409/fb64_3.189 .

Pejić, Snežana, Stojiljković, Vesna, Todorović, Ana, Gavrilović, Ljubica, Pavlović, Ivan, Popović, Nataša M., Pajović, Snežana B., "Antioxidant Enzymes in Brain Cortex of Rats Exposed to Acute, Chronic and Combined Stress" in Folia Biologica (Krakow), 64, no. 3 (2016):189-195,
https://doi.org/10.3409/fb64_3.189 . .

TY  - JOUR
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1455
AB  - Endometrial tissue is under a strong influence of sex hormones. These hormones are considered as developmental factors of endometrial hyperplasia and endometrial cancer. We examined the influence of gonadotropins (follicle-stimulating and luteinizing hormone) and sex hormones (estradiol, progesterone) on oxidant/antioxidant parameters in blood and endometrial tissue of women with complex endometrial hyperplasia. In blood, superoxide dismutase activity was significantly higher in luteal phase and postmenopause compared to the follicular phase. A significant phase-related difference of glutathione peroxidase and glutathione reductase activity was recorded in the endometrium. Both enzymes had lower activity in luteal phase and postmenopause compared to the follicular phase. The linear regression analysis of individual hormonal variables against antioxidant parameters showed negative correlation between glutathione peroxidase activity and gonadotropin concentrations in the endometrium. The regression of hyperplastic to normal endometrium is the purpose of conservative treatment based on administration of progestogens or gonadotropin-releasing hormone analogues. Our findings indicate that gonadotropins influence the antioxidant enzymes activity in women with complex endometrial hyperplasia, which may affect disease development. Further studies are needed to clarify the molecular basis of hormone action on antioxidant system that may potentially initiate a development of treatments based onredox-dependentmechanism.
T2  - Cellular and Molecular Biology
T1  - Antioxidant status and sex hormones in women with complex endometrial hyperplasia
VL  - 62
IS  - 11
SP  - 51
EP  - 56
DO  - 10.14715/cmb/2016.62.11.9
ER  - 

@article{
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Pavlović, Ivan and Gavrilović, Ljubica and Popović, Nataša M. and Pajović, Snežana B.",
year = "2016",
abstract = "Endometrial tissue is under a strong influence of sex hormones. These hormones are considered as developmental factors of endometrial hyperplasia and endometrial cancer. We examined the influence of gonadotropins (follicle-stimulating and luteinizing hormone) and sex hormones (estradiol, progesterone) on oxidant/antioxidant parameters in blood and endometrial tissue of women with complex endometrial hyperplasia. In blood, superoxide dismutase activity was significantly higher in luteal phase and postmenopause compared to the follicular phase. A significant phase-related difference of glutathione peroxidase and glutathione reductase activity was recorded in the endometrium. Both enzymes had lower activity in luteal phase and postmenopause compared to the follicular phase. The linear regression analysis of individual hormonal variables against antioxidant parameters showed negative correlation between glutathione peroxidase activity and gonadotropin concentrations in the endometrium. The regression of hyperplastic to normal endometrium is the purpose of conservative treatment based on administration of progestogens or gonadotropin-releasing hormone analogues. Our findings indicate that gonadotropins influence the antioxidant enzymes activity in women with complex endometrial hyperplasia, which may affect disease development. Further studies are needed to clarify the molecular basis of hormone action on antioxidant system that may potentially initiate a development of treatments based onredox-dependentmechanism.",
journal = "Cellular and Molecular Biology",
title = "Antioxidant status and sex hormones in women with complex endometrial hyperplasia",
volume = "62",
number = "11",
pages = "51-56",
doi = "10.14715/cmb/2016.62.11.9"
}

Pejić, S., Todorović, A., Stojiljković, V., Pavlović, I., Gavrilović, L., Popović, N. M.,& Pajović, S. B.. (2016). Antioxidant status and sex hormones in women with complex endometrial hyperplasia. in Cellular and Molecular Biology, 62(11), 51-56.
https://doi.org/10.14715/cmb/2016.62.11.9

Pejić S, Todorović A, Stojiljković V, Pavlović I, Gavrilović L, Popović NM, Pajović SB. Antioxidant status and sex hormones in women with complex endometrial hyperplasia. in Cellular and Molecular Biology. 2016;62(11):51-56.
doi:10.14715/cmb/2016.62.11.9 .

Pejić, Snežana, Todorović, Ana, Stojiljković, Vesna, Pavlović, Ivan, Gavrilović, Ljubica, Popović, Nataša M., Pajović, Snežana B., "Antioxidant status and sex hormones in women with complex endometrial hyperplasia" in Cellular and Molecular Biology, 62, no. 11 (2016):51-56,
https://doi.org/10.14715/cmb/2016.62.11.9 . .

TY  - CHAP
AU  - Labudović Borović, Milica M.
AU  - Obradović, Milan M.
AU  - Kostić, Jelena T.
AU  - Zaletel, Ivan V.
AU  - Milašinović, Dejan G.
AU  - Stojanović, Marija T.
AU  - Mutavdžin, Slavica S.
AU  - Vesković, Milena N.
AU  - Opačić, Dragan J.
AU  - Radaković, Dejan D.
AU  - Puškaš, Nela S.
AU  - Radosavljević, Tatjana S.
AU  - Borović, Saša D.
AU  - Kojić, Zvezdana Z.
AU  - Zarić, Božidarka L.
AU  - Šćepanović, Ljiljana G.
AU  - Isenović, Esma R.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11692
AB  - Structure and functions of Na+/K+-ATPase and SERCA are described with details on their subunits, isoforms, and intracellular localization. Main regulatory mechanisms are summarized. Molecular mechanisms of cell death and heart failure are explained with the analysis of the role of Na+/K+-ATPase and SERCA in these processes. Facts are considered from a cytological, pathological, and clinical perspective with an accent to new therapeutic strategies. The aim of this contribution is an overview of functional results in a structural context.
PB  - Springer
T2  - Regulation of Membrane Na+-K+ ATPase
T1  - Myocardial Na+ K+-ATPase and SERCA: Clinical and Pathological Significance From a Cytological Perspective
SP  - 113
EP  - 144
DO  - 10.1007/978-3-319-24750-2_7
ER  - 

@inbook{
author = "Labudović Borović, Milica M. and Obradović, Milan M. and Kostić, Jelena T. and Zaletel, Ivan V. and Milašinović, Dejan G. and Stojanović, Marija T. and Mutavdžin, Slavica S. and Vesković, Milena N. and Opačić, Dragan J. and Radaković, Dejan D. and Puškaš, Nela S. and Radosavljević, Tatjana S. and Borović, Saša D. and Kojić, Zvezdana Z. and Zarić, Božidarka L. and Šćepanović, Ljiljana G. and Isenović, Esma R.",
year = "2016",
abstract = "Structure and functions of Na+/K+-ATPase and SERCA are described with details on their subunits, isoforms, and intracellular localization. Main regulatory mechanisms are summarized. Molecular mechanisms of cell death and heart failure are explained with the analysis of the role of Na+/K+-ATPase and SERCA in these processes. Facts are considered from a cytological, pathological, and clinical perspective with an accent to new therapeutic strategies. The aim of this contribution is an overview of functional results in a structural context.",
publisher = "Springer",
journal = "Regulation of Membrane Na+-K+ ATPase",
booktitle = "Myocardial Na+ K+-ATPase and SERCA: Clinical and Pathological Significance From a Cytological Perspective",
pages = "113-144",
doi = "10.1007/978-3-319-24750-2_7"
}

Labudović Borović, M. M., Obradović, M. M., Kostić, J. T., Zaletel, I. V., Milašinović, D. G., Stojanović, M. T., Mutavdžin, S. S., Vesković, M. N., Opačić, D. J., Radaković, D. D., Puškaš, N. S., Radosavljević, T. S., Borović, S. D., Kojić, Z. Z., Zarić, B. L., Šćepanović, L. G.,& Isenović, E. R.. (2016). Myocardial Na+ K+-ATPase and SERCA: Clinical and Pathological Significance From a Cytological Perspective. in Regulation of Membrane Na+-K+ ATPase
Springer., 113-144.
https://doi.org/10.1007/978-3-319-24750-2_7

Labudović Borović MM, Obradović MM, Kostić JT, Zaletel IV, Milašinović DG, Stojanović MT, Mutavdžin SS, Vesković MN, Opačić DJ, Radaković DD, Puškaš NS, Radosavljević TS, Borović SD, Kojić ZZ, Zarić BL, Šćepanović LG, Isenović ER. Myocardial Na+ K+-ATPase and SERCA: Clinical and Pathological Significance From a Cytological Perspective. in Regulation of Membrane Na+-K+ ATPase. 2016;:113-144.
doi:10.1007/978-3-319-24750-2_7 .

Labudović Borović, Milica M., Obradović, Milan M., Kostić, Jelena T., Zaletel, Ivan V., Milašinović, Dejan G., Stojanović, Marija T., Mutavdžin, Slavica S., Vesković, Milena N., Opačić, Dragan J., Radaković, Dejan D., Puškaš, Nela S., Radosavljević, Tatjana S., Borović, Saša D., Kojić, Zvezdana Z., Zarić, Božidarka L., Šćepanović, Ljiljana G., Isenović, Esma R., "Myocardial Na+ K+-ATPase and SERCA: Clinical and Pathological Significance From a Cytological Perspective" in Regulation of Membrane Na+-K+ ATPase (2016):113-144,
https://doi.org/10.1007/978-3-319-24750-2_7 . .

TY  - JOUR
AU  - Pejić, Snežana
AU  - Stojiljković, Vesna
AU  - Todorović, Ana
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/498
AB  - Cancer of the reproductive tract is an important cause of morbidity and mortality among women worldwide. In this study we evaluated the influence of diagnostic categories, age and reproductive factors on antioxidant enzymes and lipid hydroperoxides in the blood of gynaecological patients diagnosed with endometrial polyp, myoma, hyperplasia simplex, hyperplasia complex and endometrial adenocarcinoma. Multivariate regression analysis was used to assess the association of diagnosis, age, parity, abortions and abnormal uterine bleeding with the examined parameters. Diagnosis provided the best predictive model for superoxide dismutase, catalase and glutathione peroxidase activities, and also for the lipid hydroperoxide level. Abortions fitted the best predictive model for superoxide dismutase activity. A significant correlation was also found between the predictor variables themselves. This study showed that reproductive and other factors may be associated, at least partially, with antioxidant capacity and ability to defend against the oxidative damage in gynaecological patients with various diagnoses.
T2  - Folia Biologica
T1  - Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors
VL  - 61
IS  - 1
SP  - 26
EP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_vinar_498
ER  - 

@article{
author = "Pejić, Snežana and Stojiljković, Vesna and Todorović, Ana and Gavrilović, Ljubica and Popović, Nataša M. and Pavlović, Ivan and Pajović, Snežana B.",
year = "2015",
abstract = "Cancer of the reproductive tract is an important cause of morbidity and mortality among women worldwide. In this study we evaluated the influence of diagnostic categories, age and reproductive factors on antioxidant enzymes and lipid hydroperoxides in the blood of gynaecological patients diagnosed with endometrial polyp, myoma, hyperplasia simplex, hyperplasia complex and endometrial adenocarcinoma. Multivariate regression analysis was used to assess the association of diagnosis, age, parity, abortions and abnormal uterine bleeding with the examined parameters. Diagnosis provided the best predictive model for superoxide dismutase, catalase and glutathione peroxidase activities, and also for the lipid hydroperoxide level. Abortions fitted the best predictive model for superoxide dismutase activity. A significant correlation was also found between the predictor variables themselves. This study showed that reproductive and other factors may be associated, at least partially, with antioxidant capacity and ability to defend against the oxidative damage in gynaecological patients with various diagnoses.",
journal = "Folia Biologica",
title = "Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors",
volume = "61",
number = "1",
pages = "26-32",
url = "https://hdl.handle.net/21.15107/rcub_vinar_498"
}

Pejić, S., Stojiljković, V., Todorović, A., Gavrilović, L., Popović, N. M., Pavlović, I.,& Pajović, S. B.. (2015). Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors. in Folia Biologica, 61(1), 26-32.
https://hdl.handle.net/21.15107/rcub_vinar_498

Pejić S, Stojiljković V, Todorović A, Gavrilović L, Popović NM, Pavlović I, Pajović SB. Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors. in Folia Biologica. 2015;61(1):26-32.
https://hdl.handle.net/21.15107/rcub_vinar_498 .

Pejić, Snežana, Stojiljković, Vesna, Todorović, Ana, Gavrilović, Ljubica, Popović, Nataša M., Pavlović, Ivan, Pajović, Snežana B., "Antioxidant Status in Blood of Gynaecological Patients: Influence of Diagnosis and Reproductive Factors" in Folia Biologica, 61, no. 1 (2015):26-32,
https://hdl.handle.net/21.15107/rcub_vinar_498 .

TY  - JOUR
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/751
AB  - Uterine leiomyomas are benign soft-tissues tumors that arise from uterine smooth muscle tissue. Etiopathogenesis of leiomyomas is not well understood. We aimed to examine whether antioxidant enzyme activities and lipid hydroperoxides level in patients with leiomyoma are influenced by changes in sex hormones and gonadotropins (estradiol (E2), progesterone, FSH, and LH) during menstrual cycle and in postmenopause. The material consisted of blood and uterine tissue specimens. Hormone concentrations were determined and assays for superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activities and lipid hydroperoxides concentration were performed. In blood of examined women, a significant difference in catalase, glutathione peroxidase and glutathione reductase activity was recorded among the phases. There was also a positive correlation between the estradiol/progesterone concentration and the catalase activity. Progesterone negatively correlated with lipid hydroperoxides level. In myoma tissue, we recorded a phase-related difference in lipid hydroperoxides level and activities of superoxide dismutase, glutathione peroxidase activities, and glutathione reductase. Negative correlation was observed between FSH and glutathione peroxidase. The results suggest that antioxidant status in patients with uterine leiomyoma is influenced by the changes in sex hormones during the menstrual cycle and in postmenopause, indicating a role of the observed relationship in the leiomyoma etiology.
T2  - Anais de Academia Brasileira de Ciencias
T1  - Antioxidant status in women with uterine leiomyoma: relation with sex hormones
VL  - 87
IS  - 3
SP  - 1771
EP  - 1782
DO  - 10.1590/0001-3765201520130416
ER  - 

@article{
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Pajović, Snežana B.",
year = "2015",
abstract = "Uterine leiomyomas are benign soft-tissues tumors that arise from uterine smooth muscle tissue. Etiopathogenesis of leiomyomas is not well understood. We aimed to examine whether antioxidant enzyme activities and lipid hydroperoxides level in patients with leiomyoma are influenced by changes in sex hormones and gonadotropins (estradiol (E2), progesterone, FSH, and LH) during menstrual cycle and in postmenopause. The material consisted of blood and uterine tissue specimens. Hormone concentrations were determined and assays for superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activities and lipid hydroperoxides concentration were performed. In blood of examined women, a significant difference in catalase, glutathione peroxidase and glutathione reductase activity was recorded among the phases. There was also a positive correlation between the estradiol/progesterone concentration and the catalase activity. Progesterone negatively correlated with lipid hydroperoxides level. In myoma tissue, we recorded a phase-related difference in lipid hydroperoxides level and activities of superoxide dismutase, glutathione peroxidase activities, and glutathione reductase. Negative correlation was observed between FSH and glutathione peroxidase. The results suggest that antioxidant status in patients with uterine leiomyoma is influenced by the changes in sex hormones during the menstrual cycle and in postmenopause, indicating a role of the observed relationship in the leiomyoma etiology.",
journal = "Anais de Academia Brasileira de Ciencias",
title = "Antioxidant status in women with uterine leiomyoma: relation with sex hormones",
volume = "87",
number = "3",
pages = "1771-1782",
doi = "10.1590/0001-3765201520130416"
}

Pejić, S., Todorović, A., Stojiljković, V., Gavrilović, L., Popović, N. M.,& Pajović, S. B.. (2015). Antioxidant status in women with uterine leiomyoma: relation with sex hormones. in Anais de Academia Brasileira de Ciencias, 87(3), 1771-1782.
https://doi.org/10.1590/0001-3765201520130416

Pejić S, Todorović A, Stojiljković V, Gavrilović L, Popović NM, Pajović SB. Antioxidant status in women with uterine leiomyoma: relation with sex hormones. in Anais de Academia Brasileira de Ciencias. 2015;87(3):1771-1782.
doi:10.1590/0001-3765201520130416 .

Pejić, Snežana, Todorović, Ana, Stojiljković, Vesna, Gavrilović, Ljubica, Popović, Nataša M., Pajović, Snežana B., "Antioxidant status in women with uterine leiomyoma: relation with sex hormones" in Anais de Academia Brasileira de Ciencias, 87, no. 3 (2015):1771-1782,
https://doi.org/10.1590/0001-3765201520130416 . .

TY  - JOUR
AU  - Todorović, Ana
AU  - Pejić, Snežana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pavlović, Ivan
AU  - Saičić, Zorica S.
AU  - Pajović, Snežana B.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/815
AB  - Purpose Previously, we examined manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD), and catalase (CAT) activities in rat brain irradiated with 2 or 3 Gy of gamma-rays. The results indicated that lower MnSOD activity and inducibility found in hippocampus might explain higher radiosensitivity of this brain region. Thus, in this study, we wanted to determine changes of MnSOD, CuZnSOD, and CAT activities after dose of 5 Gy and to find out if differences in MnSOD activity are caused by changes in its expression. Heads of 4-day-old female rats were irradiated with gamma-rays, using Co-60. Animals were sacrificed 1/24 h after exposure. Hippocampus and cortex tissues were prepared for enzyme activity measurements and Western blot analysis. One hour after exposure, gamma-rays significantly decreased MnSOD activity in both examined brain regions. Twenty-four hours later, MnSOD recovery showed dose and regional dependence. It was weaker at higher doses and in hippocampal region. MnSOD expression changed in the similar manner as MnSOD activity only at lower doses of gamma-rays. In both examined brain regions, gamma radiation significantly decreased CuZnSOD activity and did not change activity of CAT. Our results confirmed that MnSOD plays an important role in different regional radiosensitivity but also showed that depending on dose, radiation affects MnSOD level by utterly different mechanisms. Postradiation changes of CuZnSOD and CAT are not regionally specific and therefore, cannot account for the different radiosensitivity of the hippocampus and cortex.
PB  - Springer Nature
T2  - Childs Nervous System
T1  - Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity
VL  - 31
IS  - 12
SP  - 2249
EP  - 2256
DO  - 10.1007/s00381-015-2807-2
ER  - 

@article{
author = "Todorović, Ana and Pejić, Snežana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Pavlović, Ivan and Saičić, Zorica S. and Pajović, Snežana B.",
year = "2015",
abstract = "Purpose Previously, we examined manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD), and catalase (CAT) activities in rat brain irradiated with 2 or 3 Gy of gamma-rays. The results indicated that lower MnSOD activity and inducibility found in hippocampus might explain higher radiosensitivity of this brain region. Thus, in this study, we wanted to determine changes of MnSOD, CuZnSOD, and CAT activities after dose of 5 Gy and to find out if differences in MnSOD activity are caused by changes in its expression. Heads of 4-day-old female rats were irradiated with gamma-rays, using Co-60. Animals were sacrificed 1/24 h after exposure. Hippocampus and cortex tissues were prepared for enzyme activity measurements and Western blot analysis. One hour after exposure, gamma-rays significantly decreased MnSOD activity in both examined brain regions. Twenty-four hours later, MnSOD recovery showed dose and regional dependence. It was weaker at higher doses and in hippocampal region. MnSOD expression changed in the similar manner as MnSOD activity only at lower doses of gamma-rays. In both examined brain regions, gamma radiation significantly decreased CuZnSOD activity and did not change activity of CAT. Our results confirmed that MnSOD plays an important role in different regional radiosensitivity but also showed that depending on dose, radiation affects MnSOD level by utterly different mechanisms. Postradiation changes of CuZnSOD and CAT are not regionally specific and therefore, cannot account for the different radiosensitivity of the hippocampus and cortex.",
publisher = "Springer Nature",
journal = "Childs Nervous System",
title = "Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity",
volume = "31",
number = "12",
pages = "2249-2256",
doi = "10.1007/s00381-015-2807-2"
}

Todorović, A., Pejić, S., Stojiljković, V., Gavrilović, L., Popović, N. M., Pavlović, I., Saičić, Z. S.,& Pajović, S. B.. (2015). Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity. in Childs Nervous System
Springer Nature., 31(12), 2249-2256.
https://doi.org/10.1007/s00381-015-2807-2

Todorović A, Pejić S, Stojiljković V, Gavrilović L, Popović NM, Pavlović I, Saičić ZS, Pajović SB. Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity. in Childs Nervous System. 2015;31(12):2249-2256.
doi:10.1007/s00381-015-2807-2 .

Todorović, Ana, Pejić, Snežana, Stojiljković, Vesna, Gavrilović, Ljubica, Popović, Nataša M., Pavlović, Ivan, Saičić, Zorica S., Pajović, Snežana B., "Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity" in Childs Nervous System, 31, no. 12 (2015):2249-2256,
https://doi.org/10.1007/s00381-015-2807-2 . .

TY  - CONF
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pavlović, Ivan
AU  - Pajović, Snežana B.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7131
AB  - Endometrial hyperplasia complex is gynecological disorder characterized by morphological irregularities of glands shape and size. Antioxidant enzymes (AOE), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), have an essential role in preventing oxidative damage in cell caused by reactive oxygen species (ROS). In this study, we examined the AO status in hyperplastic tissue of patients in menstrual cycle (follicular and luteal phase) and in postmenopause, as well as the relationship between sex hormones and AO parameters. The phase-related activity of GPx and GR in examined patients was significantly different than in healthy women. A significant negative correlation between FSH/LH level and GPx activiy was observed. Endometrial hyperplasias are considered as precancerous lesions and are treated either conservatively or surgicaly, and also by radiation therapy. Since the effects of these therapies are associated with AO and hormonal changes, our results may contribute to the prediction of potential therapeutic efficacy and to selection of the most effective treatment for hyperplasia complex.
C3  - RAD Conference Proceedings
T1  - Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones
SP  - 467
EP  - 470
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7131
ER  - 

@conference{
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Pavlović, Ivan and Pajović, Snežana B.",
year = "2015",
abstract = "Endometrial hyperplasia complex is gynecological disorder characterized by morphological irregularities of glands shape and size. Antioxidant enzymes (AOE), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), have an essential role in preventing oxidative damage in cell caused by reactive oxygen species (ROS). In this study, we examined the AO status in hyperplastic tissue of patients in menstrual cycle (follicular and luteal phase) and in postmenopause, as well as the relationship between sex hormones and AO parameters. The phase-related activity of GPx and GR in examined patients was significantly different than in healthy women. A significant negative correlation between FSH/LH level and GPx activiy was observed. Endometrial hyperplasias are considered as precancerous lesions and are treated either conservatively or surgicaly, and also by radiation therapy. Since the effects of these therapies are associated with AO and hormonal changes, our results may contribute to the prediction of potential therapeutic efficacy and to selection of the most effective treatment for hyperplasia complex.",
journal = "RAD Conference Proceedings",
title = "Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones",
pages = "467-470",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7131"
}

Pejić, S., Todorović, A., Stojiljković, V., Gavrilović, L., Popović, N. M., Pavlović, I.,& Pajović, S. B.. (2015). Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones. in RAD Conference Proceedings, 467-470.
https://hdl.handle.net/21.15107/rcub_vinar_7131

Pejić S, Todorović A, Stojiljković V, Gavrilović L, Popović NM, Pavlović I, Pajović SB. Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones. in RAD Conference Proceedings. 2015;:467-470.
https://hdl.handle.net/21.15107/rcub_vinar_7131 .

Pejić, Snežana, Todorović, Ana, Stojiljković, Vesna, Gavrilović, Ljubica, Popović, Nataša M., Pavlović, Ivan, Pajović, Snežana B., "Antioxidant Enzymes in Women with Hyperplasia Complex: Relation with Sex Hormones" in RAD Conference Proceedings (2015):467-470,
https://hdl.handle.net/21.15107/rcub_vinar_7131 .

TY  - CHAP
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Pavlović, Ivan
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8408
AB  - Cancer of the reproductive tract is an important source of morbidity and mortality among women worldwide. Factors affecting endometrial cancer and endometrial hyperplasia are known to be similar. Endometrial hyperplasia is abnormal proliferation of the glands and the stroma resulting in architectural and cytological modifications. Due to hormonal changes, this condition is most common among women who are nearing the menopause or have reached the menopause. Antioxidant system has a role in preventing cancer initiation and promotion. Since the carcinogenesis occurs in several stages, it is likely that the antioxidant defense depends on the type of cell and tissue. The objective of this study was to investigate whether antioxidant enzymes activities and lipid hydroperoxides concentration in patients with endometrial hyperplasia are influenced by the changes in sex hormones level (estradiol, progesterone, FSH, and LH) during the menstrual cycle and in postmenopause. The material we used consisted of blood and endometrial tissue specimens of women diagnosed with endometrial hyperplasia simplex. Patients were divided in groups depending on the phase of the menstrual cycle: follicular phase, luteal phase and postmenopause. The activities of antioxidant enzymes and the lipid hydroperoxides level were compared among the phases to test the differences and a linear regression model was used to evaluate the associations between hormone levels and antioxidant/oxidant variables. In the blood of examined patients, we observed a phase-related changes of LOOH concentrations. Significant negative correlation between FSH concentration and GR activity (r= -0.42, p<0.05) and significant positive correlation between LH and LOOH concentrations (r= 0.038, p<0.05) was found. In hyperplasia simplex tissue we recorded significant phase-related changes of LOOH level as well as of AO enzyme activities. SOD and CAT had similar activity pattern, which was higher in luteal phase and in postmenopause, compared to follicular phase (p<0.05). GPx and GR activities did not show any statistical difference. Also, negative correlation between progesterone and GR activity (r=-0.036, p<0.05) was observed. Hormonal influence on AO system is of importance in gynecological diseases etiology since they may promote cell proliferation but are also used in conservative therapy, especially for hyperplasia simplex. However, the role of ROS production as a risk factor for endometrial hyperplasia still needs to be clarified as well as the role of AO status in response to gonadotropins and sex steroids.
T2  - Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder
T1  - Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia
IS  - Ch. 9
SP  - 243
EP  - 279
DO  - 10.5772/60853
ER  - 

@inbook{
editor = "Gowder, Sivakumar Joghi Thatha",
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Pavlović, Ivan and Gavrilović, Ljubica and Popović, Nataša M. and Pajović, Snežana B.",
year = "2015",
abstract = "Cancer of the reproductive tract is an important source of morbidity and mortality among women worldwide. Factors affecting endometrial cancer and endometrial hyperplasia are known to be similar. Endometrial hyperplasia is abnormal proliferation of the glands and the stroma resulting in architectural and cytological modifications. Due to hormonal changes, this condition is most common among women who are nearing the menopause or have reached the menopause. Antioxidant system has a role in preventing cancer initiation and promotion. Since the carcinogenesis occurs in several stages, it is likely that the antioxidant defense depends on the type of cell and tissue. The objective of this study was to investigate whether antioxidant enzymes activities and lipid hydroperoxides concentration in patients with endometrial hyperplasia are influenced by the changes in sex hormones level (estradiol, progesterone, FSH, and LH) during the menstrual cycle and in postmenopause. The material we used consisted of blood and endometrial tissue specimens of women diagnosed with endometrial hyperplasia simplex. Patients were divided in groups depending on the phase of the menstrual cycle: follicular phase, luteal phase and postmenopause. The activities of antioxidant enzymes and the lipid hydroperoxides level were compared among the phases to test the differences and a linear regression model was used to evaluate the associations between hormone levels and antioxidant/oxidant variables. In the blood of examined patients, we observed a phase-related changes of LOOH concentrations. Significant negative correlation between FSH concentration and GR activity (r= -0.42, p<0.05) and significant positive correlation between LH and LOOH concentrations (r= 0.038, p<0.05) was found. In hyperplasia simplex tissue we recorded significant phase-related changes of LOOH level as well as of AO enzyme activities. SOD and CAT had similar activity pattern, which was higher in luteal phase and in postmenopause, compared to follicular phase (p<0.05). GPx and GR activities did not show any statistical difference. Also, negative correlation between progesterone and GR activity (r=-0.036, p<0.05) was observed. Hormonal influence on AO system is of importance in gynecological diseases etiology since they may promote cell proliferation but are also used in conservative therapy, especially for hyperplasia simplex. However, the role of ROS production as a risk factor for endometrial hyperplasia still needs to be clarified as well as the role of AO status in response to gonadotropins and sex steroids.",
journal = "Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder",
booktitle = "Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia",
number = "Ch. 9",
pages = "243-279",
doi = "10.5772/60853"
}

Gowder, S. J. T., Pejić, S., Todorović, A., Stojiljković, V., Pavlović, I., Gavrilović, L., Popović, N. M.,& Pajović, S. B.. (2015). Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia. in Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder(Ch. 9), 243-279.
https://doi.org/10.5772/60853

Gowder SJT, Pejić S, Todorović A, Stojiljković V, Pavlović I, Gavrilović L, Popović NM, Pajović SB. Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia. in Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder. 2015;(Ch. 9):243-279.
doi:10.5772/60853 .

Gowder, Sivakumar Joghi Thatha, Pejić, Snežana, Todorović, Ana, Stojiljković, Vesna, Pavlović, Ivan, Gavrilović, Ljubica, Popović, Nataša M., Pajović, Snežana B., "Antioxidant Status and Sex Hormones in Women with Simple Endometrial Hyperplasia" in Basic Principles and Clinical Significance of Oxidative Stress, (ed.) Sivakumar Joghi Thatha Gowder, no. Ch. 9 (2015):243-279,
https://doi.org/10.5772/60853 . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Kasapović, Jelena
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
AU  - Dronjak, Slađana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5703
AB  - Chronic isolation of adult animals represents a form of psychological stress that produces sympatho-adrenomedullar activation. Exercise training acts as an important modulator of sympatho-adrenomedullary system. This study aimed to investigate physical exercise-related changes in gene expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase, dopamine-beta-hydroxylase and phenylethanolamine N-methyltransferase) and cyclic adenosine monophosphate response element-binding (CREB) in the adrenal medulla, concentrations of catecholamines and corticosterone (CORT) in the plasma and the weight of adrenal glands of chronically psychosocially stressed adult rats exposed daily to 20 min treadmill running for 12 weeks, Also, we examined how additional acute immobilization stress changes the mentioned parameters. Treadmill running did not result in modulation of gene expression of catecholamine synthesizing enzymes and it decreased the level of CREB mRNA in the adrenal medulla of chronically psychosocially stressed adult rats. The potentially negative physiological adaptations after treadmill running were recorded as increased concentrations of catecholamines and decreased morning CORT concentration in the plasma, as well as the adrenal gland hypertrophy of chronically psychosocially stressed rats. The additional acute immobilization stress increases gene expression of catecholamine biosynthetic enzymes in the adrenal medulla, as well as catecholamines and CORT levels in the plasma. Treadmill exercise does not change the activity of sympatho-adrenomedullary system of chronically psychosocially stressed rats.
T2  - Anais de Academia Brasileira de Ciencias
T1  - Treadmill exercise does not change gene expression of adrenal catecholamine biosynthetic enzymes in chronically stressed rats
VL  - 85
IS  - 3
SP  - 999
EP  - 1012
DO  - 10.1590/S0001-37652013005000041
ER  - 

@article{
author = "Gavrilović, Ljubica and Stojiljković, Vesna and Kasapović, Jelena and Popović, Nataša M. and Pajović, Snežana B. and Dronjak, Slađana",
year = "2013",
abstract = "Chronic isolation of adult animals represents a form of psychological stress that produces sympatho-adrenomedullar activation. Exercise training acts as an important modulator of sympatho-adrenomedullary system. This study aimed to investigate physical exercise-related changes in gene expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase, dopamine-beta-hydroxylase and phenylethanolamine N-methyltransferase) and cyclic adenosine monophosphate response element-binding (CREB) in the adrenal medulla, concentrations of catecholamines and corticosterone (CORT) in the plasma and the weight of adrenal glands of chronically psychosocially stressed adult rats exposed daily to 20 min treadmill running for 12 weeks, Also, we examined how additional acute immobilization stress changes the mentioned parameters. Treadmill running did not result in modulation of gene expression of catecholamine synthesizing enzymes and it decreased the level of CREB mRNA in the adrenal medulla of chronically psychosocially stressed adult rats. The potentially negative physiological adaptations after treadmill running were recorded as increased concentrations of catecholamines and decreased morning CORT concentration in the plasma, as well as the adrenal gland hypertrophy of chronically psychosocially stressed rats. The additional acute immobilization stress increases gene expression of catecholamine biosynthetic enzymes in the adrenal medulla, as well as catecholamines and CORT levels in the plasma. Treadmill exercise does not change the activity of sympatho-adrenomedullary system of chronically psychosocially stressed rats.",
journal = "Anais de Academia Brasileira de Ciencias",
title = "Treadmill exercise does not change gene expression of adrenal catecholamine biosynthetic enzymes in chronically stressed rats",
volume = "85",
number = "3",
pages = "999-1012",
doi = "10.1590/S0001-37652013005000041"
}

Gavrilović, L., Stojiljković, V., Kasapović, J., Popović, N. M., Pajović, S. B.,& Dronjak, S.. (2013). Treadmill exercise does not change gene expression of adrenal catecholamine biosynthetic enzymes in chronically stressed rats. in Anais de Academia Brasileira de Ciencias, 85(3), 999-1012.
https://doi.org/10.1590/S0001-37652013005000041

Gavrilović L, Stojiljković V, Kasapović J, Popović NM, Pajović SB, Dronjak S. Treadmill exercise does not change gene expression of adrenal catecholamine biosynthetic enzymes in chronically stressed rats. in Anais de Academia Brasileira de Ciencias. 2013;85(3):999-1012.
doi:10.1590/S0001-37652013005000041 .

Gavrilović, Ljubica, Stojiljković, Vesna, Kasapović, Jelena, Popović, Nataša M., Pajović, Snežana B., Dronjak, Slađana, "Treadmill exercise does not change gene expression of adrenal catecholamine biosynthetic enzymes in chronically stressed rats" in Anais de Academia Brasileira de Ciencias, 85, no. 3 (2013):999-1012,
https://doi.org/10.1590/S0001-37652013005000041 . .

TY  - JOUR
AU  - Pejić, Snežana
AU  - Kasapović, Jelena
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Gavrilović, Ljubica
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5705
AB  - Objective: To investigate whether antioxidant enzyme activities (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase) and lipid hydroperoxide levels in patients with endometrial polyps are influenced by the changes in sex hormones (estradiol, progesterone, FSH, and LH) during the menstrual cycle and in postmenopause. Study design: The material consisted of blood and endometrial tissue specimens from women diagnosed with endometrial polyps. Patients were divided into groups depending on the phase of the menstrual cycle - follicular or luteal - and the postmenopause. The activities of antioxidant enzymes and the lipid hydroperoxide levels were compared among the phases and a linear regression model was used to evaluate the associations between hormones and antioxidant/oxidant variables. Results: In the blood of examined women, a significant difference in superoxide dismutase activity and lipid hydroperoxide levels was recorded among the phases. There was also a positive correlation between the estradiol concentration and superoxide dismutase. In polyp tissue, we recorded a phase-related difference in superoxide dismutase and glutathione peroxidase activities as well as in the lipid hydroperoxide levels. A negative correlation was observed between FSH/LH and glutathione peroxidase, and between LH and superoxide dismutase. Conclusion: Antioxidant enzymes and lipid hydroperoxide levels in patients with endometrial polyps are influenced by the changes in sex hormones during the menstrual cycle and after the menopause, pointing to a role of the observed relationship in polyp etiology. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
T2  - European Journal of Obstetrics Gynecology and Reproductive Biology
T1  - Antioxidant enzymes in women with endometrial polyps: relation with sex hormones
VL  - 170
IS  - 1
SP  - 241
EP  - 246
DO  - 10.1016/j.ejogrb.2013.06.024
ER  - 

@article{
author = "Pejić, Snežana and Kasapović, Jelena and Todorović, Ana and Stojiljković, Vesna and Gavrilović, Ljubica and Popović, Nataša M. and Pajović, Snežana B.",
year = "2013",
abstract = "Objective: To investigate whether antioxidant enzyme activities (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase) and lipid hydroperoxide levels in patients with endometrial polyps are influenced by the changes in sex hormones (estradiol, progesterone, FSH, and LH) during the menstrual cycle and in postmenopause. Study design: The material consisted of blood and endometrial tissue specimens from women diagnosed with endometrial polyps. Patients were divided into groups depending on the phase of the menstrual cycle - follicular or luteal - and the postmenopause. The activities of antioxidant enzymes and the lipid hydroperoxide levels were compared among the phases and a linear regression model was used to evaluate the associations between hormones and antioxidant/oxidant variables. Results: In the blood of examined women, a significant difference in superoxide dismutase activity and lipid hydroperoxide levels was recorded among the phases. There was also a positive correlation between the estradiol concentration and superoxide dismutase. In polyp tissue, we recorded a phase-related difference in superoxide dismutase and glutathione peroxidase activities as well as in the lipid hydroperoxide levels. A negative correlation was observed between FSH/LH and glutathione peroxidase, and between LH and superoxide dismutase. Conclusion: Antioxidant enzymes and lipid hydroperoxide levels in patients with endometrial polyps are influenced by the changes in sex hormones during the menstrual cycle and after the menopause, pointing to a role of the observed relationship in polyp etiology. (C) 2013 Elsevier Ireland Ltd. All rights reserved.",
journal = "European Journal of Obstetrics Gynecology and Reproductive Biology",
title = "Antioxidant enzymes in women with endometrial polyps: relation with sex hormones",
volume = "170",
number = "1",
pages = "241-246",
doi = "10.1016/j.ejogrb.2013.06.024"
}

Pejić, S., Kasapović, J., Todorović, A., Stojiljković, V., Gavrilović, L., Popović, N. M.,& Pajović, S. B.. (2013). Antioxidant enzymes in women with endometrial polyps: relation with sex hormones. in European Journal of Obstetrics Gynecology and Reproductive Biology, 170(1), 241-246.
https://doi.org/10.1016/j.ejogrb.2013.06.024

Pejić S, Kasapović J, Todorović A, Stojiljković V, Gavrilović L, Popović NM, Pajović SB. Antioxidant enzymes in women with endometrial polyps: relation with sex hormones. in European Journal of Obstetrics Gynecology and Reproductive Biology. 2013;170(1):241-246.
doi:10.1016/j.ejogrb.2013.06.024 .

Pejić, Snežana, Kasapović, Jelena, Todorović, Ana, Stojiljković, Vesna, Gavrilović, Ljubica, Popović, Nataša M., Pajović, Snežana B., "Antioxidant enzymes in women with endometrial polyps: relation with sex hormones" in European Journal of Obstetrics Gynecology and Reproductive Biology, 170, no. 1 (2013):241-246,
https://doi.org/10.1016/j.ejogrb.2013.06.024 . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Kasapović, Jelena
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pajović, Snežana B.
AU  - Dronjak, Slađana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5189
AB  - The aim of this study was to examine how acute immobilization stress affects the concentrations of catecholamines in the plasma and the expression of the splenic catecholamine biosynthetic enzymes tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) in chronically socially isolated rats. We found that acute immobilization increases the plasma catecholamine levels and splenic PNMT protein levels in chronically socially isolated rats. These results show that acute stress of chronically stressed animals activates the sympatho-adrenomedullary system and increases synthesis of splenic PNMT by 37%, both of which can modulate the immune function.
T2  - Archives of Biological Sciences
T1  - Effects of Acute Stress on Gene Expression of Splenic Catecholamine Biosynthetic Enzymes in Chronically Stressed Rats
VL  - 65
IS  - 1
SP  - 183
EP  - 189
DO  - 10.2298/ABS1301183G
ER  - 

@article{
author = "Gavrilović, Ljubica and Stojiljković, Vesna and Kasapović, Jelena and Pejić, Snežana and Todorović, Ana and Pajović, Snežana B. and Dronjak, Slađana",
year = "2013",
abstract = "The aim of this study was to examine how acute immobilization stress affects the concentrations of catecholamines in the plasma and the expression of the splenic catecholamine biosynthetic enzymes tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) in chronically socially isolated rats. We found that acute immobilization increases the plasma catecholamine levels and splenic PNMT protein levels in chronically socially isolated rats. These results show that acute stress of chronically stressed animals activates the sympatho-adrenomedullary system and increases synthesis of splenic PNMT by 37%, both of which can modulate the immune function.",
journal = "Archives of Biological Sciences",
title = "Effects of Acute Stress on Gene Expression of Splenic Catecholamine Biosynthetic Enzymes in Chronically Stressed Rats",
volume = "65",
number = "1",
pages = "183-189",
doi = "10.2298/ABS1301183G"
}

Gavrilović, L., Stojiljković, V., Kasapović, J., Pejić, S., Todorović, A., Pajović, S. B.,& Dronjak, S.. (2013). Effects of Acute Stress on Gene Expression of Splenic Catecholamine Biosynthetic Enzymes in Chronically Stressed Rats. in Archives of Biological Sciences, 65(1), 183-189.
https://doi.org/10.2298/ABS1301183G

Gavrilović L, Stojiljković V, Kasapović J, Pejić S, Todorović A, Pajović SB, Dronjak S. Effects of Acute Stress on Gene Expression of Splenic Catecholamine Biosynthetic Enzymes in Chronically Stressed Rats. in Archives of Biological Sciences. 2013;65(1):183-189.
doi:10.2298/ABS1301183G .

Gavrilović, Ljubica, Stojiljković, Vesna, Kasapović, Jelena, Pejić, Snežana, Todorović, Ana, Pajović, Snežana B., Dronjak, Slađana, "Effects of Acute Stress on Gene Expression of Splenic Catecholamine Biosynthetic Enzymes in Chronically Stressed Rats" in Archives of Biological Sciences, 65, no. 1 (2013):183-189,
https://doi.org/10.2298/ABS1301183G . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Kasapović, Jelena
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pajović, Snežana B.
AU  - Dronjak, Slađana
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4915
AB  - In this study we examined how chronic forced running (CFR) affects the expression of catecholamine biosynthetic enzymes and cAMP response element-binding (CREB) in the adrenal medulla and the weight of adrenal glands of rats. Also, we examined how CFR and additional acute immobilization stress affect the expression of catecholamine biosynthetic enzymes in the adrenal medulla and the concentration of catecholamines and corticosterone (CORT) in the blood plasma. In this experiment we used as a model forced exercise in rats (treadmill running). We used the most advanced method for determining the level of gene expression, Real-time PCR with TaqMan probes, as well as Western blot analysis (ECL). We found that CFR decreases tyrosine hydroxylase (TH), and dopamine-beta-hydroxylase (DBH) mRNA and protein levels in the adrenal medulla. The decreased TH and DBH mRNA levels coincide with the reduced expression of CREB in the adrenal medulla and with the reduced plasma CORT level. Additionally, CFR reduces the level of phenylethanolamine N-methyltransferase (PNMT) mRNA, but elevates its protein level in the adrenal medulla and increases the concentration of adrenaline (A) in the plasma. Reduced level of PNMT mRNA in the adrenal medulla coincides with reduced plasma CORT level. The additional acute immobilization stress increases gene expression of catecholamine biosynthetic enzymes in the adrenal medulla, as well as catecholamines and CORT levels in the plasma. The increased synthesis of PNMT enzyme in the adrenal medulla may result in an increased biosynthesis of A under chronic stress conditions. Additionally, increased level of catecholamines in the plasma after chronic physical stress is the allostatic load that may induce numerous diseases and pathological conditions.
T2  - Acta Veterinaria, Beograd
T1  - Chronic Physical Stress Changes Gene Expression of Catecholamine Biosynthetic Enzymes in the Adrenal Medulla of Adult Rats
VL  - 62
IS  - 2-3
SP  - 151
EP  - 169
DO  - 10.2298/AVB1203151G
ER  - 

@article{
author = "Gavrilović, Ljubica and Stojiljković, Vesna and Kasapović, Jelena and Pejić, Snežana and Todorović, Ana and Pajović, Snežana B. and Dronjak, Slađana",
year = "2012",
abstract = "In this study we examined how chronic forced running (CFR) affects the expression of catecholamine biosynthetic enzymes and cAMP response element-binding (CREB) in the adrenal medulla and the weight of adrenal glands of rats. Also, we examined how CFR and additional acute immobilization stress affect the expression of catecholamine biosynthetic enzymes in the adrenal medulla and the concentration of catecholamines and corticosterone (CORT) in the blood plasma. In this experiment we used as a model forced exercise in rats (treadmill running). We used the most advanced method for determining the level of gene expression, Real-time PCR with TaqMan probes, as well as Western blot analysis (ECL). We found that CFR decreases tyrosine hydroxylase (TH), and dopamine-beta-hydroxylase (DBH) mRNA and protein levels in the adrenal medulla. The decreased TH and DBH mRNA levels coincide with the reduced expression of CREB in the adrenal medulla and with the reduced plasma CORT level. Additionally, CFR reduces the level of phenylethanolamine N-methyltransferase (PNMT) mRNA, but elevates its protein level in the adrenal medulla and increases the concentration of adrenaline (A) in the plasma. Reduced level of PNMT mRNA in the adrenal medulla coincides with reduced plasma CORT level. The additional acute immobilization stress increases gene expression of catecholamine biosynthetic enzymes in the adrenal medulla, as well as catecholamines and CORT levels in the plasma. The increased synthesis of PNMT enzyme in the adrenal medulla may result in an increased biosynthesis of A under chronic stress conditions. Additionally, increased level of catecholamines in the plasma after chronic physical stress is the allostatic load that may induce numerous diseases and pathological conditions.",
journal = "Acta Veterinaria, Beograd",
title = "Chronic Physical Stress Changes Gene Expression of Catecholamine Biosynthetic Enzymes in the Adrenal Medulla of Adult Rats",
volume = "62",
number = "2-3",
pages = "151-169",
doi = "10.2298/AVB1203151G"
}

Gavrilović, L., Stojiljković, V., Kasapović, J., Pejić, S., Todorović, A., Pajović, S. B.,& Dronjak, S.. (2012). Chronic Physical Stress Changes Gene Expression of Catecholamine Biosynthetic Enzymes in the Adrenal Medulla of Adult Rats. in Acta Veterinaria, Beograd, 62(2-3), 151-169.
https://doi.org/10.2298/AVB1203151G

Gavrilović L, Stojiljković V, Kasapović J, Pejić S, Todorović A, Pajović SB, Dronjak S. Chronic Physical Stress Changes Gene Expression of Catecholamine Biosynthetic Enzymes in the Adrenal Medulla of Adult Rats. in Acta Veterinaria, Beograd. 2012;62(2-3):151-169.
doi:10.2298/AVB1203151G .

Gavrilović, Ljubica, Stojiljković, Vesna, Kasapović, Jelena, Pejić, Snežana, Todorović, Ana, Pajović, Snežana B., Dronjak, Slađana, "Chronic Physical Stress Changes Gene Expression of Catecholamine Biosynthetic Enzymes in the Adrenal Medulla of Adult Rats" in Acta Veterinaria, Beograd, 62, no. 2-3 (2012):151-169,
https://doi.org/10.2298/AVB1203151G . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Kasapović, Jelena
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Pajović, Snežana B.
AU  - Dronjak, Slađana
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5057
AB  - Treadmill training produces modulation of neuro-endocrine and immune functions. This study examined the effects of chronic forced running (CFR) on the plasma concentration of catecholamines and the expression of splenic catecholamine biosynthetic enzymes in rats by using real-time RT-PCR and Western blot analyses. We found that CFR increases the plasma catecholamine levels, decreases splenic tyrosine hydroxylase (TH), dopamine-p-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) mRNA levels and increases splenic PNMT protein levels. This shows that CFR is a very strong stressor which activates the sympatho-adrenomedullary system and increases synthesis of splenic PNMT by 20%, which both can modulate the immune function. (C) 2012 Elsevier B.V. All rights reserved.
T2  - Journal of Neuroimmunology
T1  - Forced exercise changes catecholamine synthesis in the spleen of adult rats
VL  - 251
IS  - 1-2
SP  - 1
EP  - 5
DO  - 10.1016/j.jneuroim.2012.06.002
ER  - 

@article{
author = "Gavrilović, Ljubica and Stojiljković, Vesna and Kasapović, Jelena and Pejić, Snežana and Todorović, Ana and Pajović, Snežana B. and Dronjak, Slađana",
year = "2012",
abstract = "Treadmill training produces modulation of neuro-endocrine and immune functions. This study examined the effects of chronic forced running (CFR) on the plasma concentration of catecholamines and the expression of splenic catecholamine biosynthetic enzymes in rats by using real-time RT-PCR and Western blot analyses. We found that CFR increases the plasma catecholamine levels, decreases splenic tyrosine hydroxylase (TH), dopamine-p-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) mRNA levels and increases splenic PNMT protein levels. This shows that CFR is a very strong stressor which activates the sympatho-adrenomedullary system and increases synthesis of splenic PNMT by 20%, which both can modulate the immune function. (C) 2012 Elsevier B.V. All rights reserved.",
journal = "Journal of Neuroimmunology",
title = "Forced exercise changes catecholamine synthesis in the spleen of adult rats",
volume = "251",
number = "1-2",
pages = "1-5",
doi = "10.1016/j.jneuroim.2012.06.002"
}

Gavrilović, L., Stojiljković, V., Kasapović, J., Pejić, S., Todorović, A., Pajović, S. B.,& Dronjak, S.. (2012). Forced exercise changes catecholamine synthesis in the spleen of adult rats. in Journal of Neuroimmunology, 251(1-2), 1-5.
https://doi.org/10.1016/j.jneuroim.2012.06.002

Gavrilović L, Stojiljković V, Kasapović J, Pejić S, Todorović A, Pajović SB, Dronjak S. Forced exercise changes catecholamine synthesis in the spleen of adult rats. in Journal of Neuroimmunology. 2012;251(1-2):1-5.
doi:10.1016/j.jneuroim.2012.06.002 .

Gavrilović, Ljubica, Stojiljković, Vesna, Kasapović, Jelena, Pejić, Snežana, Todorović, Ana, Pajović, Snežana B., Dronjak, Slađana, "Forced exercise changes catecholamine synthesis in the spleen of adult rats" in Journal of Neuroimmunology, 251, no. 1-2 (2012):1-5,
https://doi.org/10.1016/j.jneuroim.2012.06.002 . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Mandušić, Vesna
AU  - Stojiljković, Vesna
AU  - Kasapović, Jelena
AU  - Stojiljković, S.
AU  - Pajović, Snežana B.
AU  - Dronjak, Slađana
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5112
AB  - The sympathoneural system has a profound influence on the heart function. Sympathetic neurons are the major contributors to the huge rise of circulating noradrenaline (NA) level in response to stressful stimuli. Treadmill training in rats is forced exercise which has the propensity to induce both psychological and physical stress. The aim of this study is to examine how chronic forced running (CFR) affects the expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT)) and cAMP response element-binding (CREB) in stellate ganglia, as well as the concentrations of catecholamines, adrenocorticotropic hormone (ACTH) and corticosterone (CORT) in the plasma of rats. Also, we investigated how the additional acute immobilization stress changes the mentioned parameters. The rat training program consisted of 12 weeks running on a treadmill (20 m/min, 20 min/day). We found that CFR increases TH and DBH mRNA and protein levels in stellate ganglia, which is followed by increased NA concentration in the plasma. CFR reduces the level of PNMT m RNA, while the level of PNMT protein remains unchanged in stellate ganglia. The increased expression of TH and DBH genes positively correlates with the expression of CREB in stellate ganglia and with plasma ACTH level, while reduced level of PNMT mRNA in stellate ganglia correlates with reduced plasma CORT level. The additional acute immobilization stress increased gene expression of catecholamine biosynthetic enzymes in stellate ganglia, as well as catecholamines, ACTH and CORT levels in the plasma. The results presented here suggest that the continuous increase of the noradrenaline biosynthetic enzyme expression in stellate ganglia due to CFR may play a role in growing risk of cardiovascular diseases.
T2  - Journal of Biological Regulators and Homeostatic Agents
T1  - Effect of Chronic Forced Running on Gene Expression of Catecholamine Biosynthetic Enzymes in Stellate Ganglia of Rats
VL  - 26
IS  - 3
SP  - 367
EP  - 377
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5112
ER  - 

@article{
author = "Gavrilović, Ljubica and Mandušić, Vesna and Stojiljković, Vesna and Kasapović, Jelena and Stojiljković, S. and Pajović, Snežana B. and Dronjak, Slađana",
year = "2012",
abstract = "The sympathoneural system has a profound influence on the heart function. Sympathetic neurons are the major contributors to the huge rise of circulating noradrenaline (NA) level in response to stressful stimuli. Treadmill training in rats is forced exercise which has the propensity to induce both psychological and physical stress. The aim of this study is to examine how chronic forced running (CFR) affects the expression of catecholamine biosynthetic enzymes (tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT)) and cAMP response element-binding (CREB) in stellate ganglia, as well as the concentrations of catecholamines, adrenocorticotropic hormone (ACTH) and corticosterone (CORT) in the plasma of rats. Also, we investigated how the additional acute immobilization stress changes the mentioned parameters. The rat training program consisted of 12 weeks running on a treadmill (20 m/min, 20 min/day). We found that CFR increases TH and DBH mRNA and protein levels in stellate ganglia, which is followed by increased NA concentration in the plasma. CFR reduces the level of PNMT m RNA, while the level of PNMT protein remains unchanged in stellate ganglia. The increased expression of TH and DBH genes positively correlates with the expression of CREB in stellate ganglia and with plasma ACTH level, while reduced level of PNMT mRNA in stellate ganglia correlates with reduced plasma CORT level. The additional acute immobilization stress increased gene expression of catecholamine biosynthetic enzymes in stellate ganglia, as well as catecholamines, ACTH and CORT levels in the plasma. The results presented here suggest that the continuous increase of the noradrenaline biosynthetic enzyme expression in stellate ganglia due to CFR may play a role in growing risk of cardiovascular diseases.",
journal = "Journal of Biological Regulators and Homeostatic Agents",
title = "Effect of Chronic Forced Running on Gene Expression of Catecholamine Biosynthetic Enzymes in Stellate Ganglia of Rats",
volume = "26",
number = "3",
pages = "367-377",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5112"
}

Gavrilović, L., Mandušić, V., Stojiljković, V., Kasapović, J., Stojiljković, S., Pajović, S. B.,& Dronjak, S.. (2012). Effect of Chronic Forced Running on Gene Expression of Catecholamine Biosynthetic Enzymes in Stellate Ganglia of Rats. in Journal of Biological Regulators and Homeostatic Agents, 26(3), 367-377.
https://hdl.handle.net/21.15107/rcub_vinar_5112

Gavrilović L, Mandušić V, Stojiljković V, Kasapović J, Stojiljković S, Pajović SB, Dronjak S. Effect of Chronic Forced Running on Gene Expression of Catecholamine Biosynthetic Enzymes in Stellate Ganglia of Rats. in Journal of Biological Regulators and Homeostatic Agents. 2012;26(3):367-377.
https://hdl.handle.net/21.15107/rcub_vinar_5112 .

Gavrilović, Ljubica, Mandušić, Vesna, Stojiljković, Vesna, Kasapović, Jelena, Stojiljković, S., Pajović, Snežana B., Dronjak, Slađana, "Effect of Chronic Forced Running on Gene Expression of Catecholamine Biosynthetic Enzymes in Stellate Ganglia of Rats" in Journal of Biological Regulators and Homeostatic Agents, 26, no. 3 (2012):367-377,
https://hdl.handle.net/21.15107/rcub_vinar_5112 .