TY  - JOUR
AU  - Paraš, Smiljana
AU  - Janković, Ognjenka
AU  - Trišić, Dijana
AU  - Čolović, Božana M.
AU  - Mitrović‐Ajtić, Olivera
AU  - Dekić, Radoslav
AU  - Soldatović, Ivan A.
AU  - Živković-Sandić, Marija
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman R.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8557
AB  - Aim: To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology: Twenty-four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t-test) and nonparametric tests (Kruskal–Wallis and Mann–Whitney U-test) depending on data distribution. Results: Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions: This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential. © 2019 International Endodontic Journal. Published by John Wiley & Sons Ltd
T2  - International Endodontic Journal
T1  - Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis
SP  - 1162
EP  - 1172
DO  - 10.1111/iej.13105
ER  - 

@article{
author = "Paraš, Smiljana and Janković, Ognjenka and Trišić, Dijana and Čolović, Božana M. and Mitrović‐Ajtić, Olivera and Dekić, Radoslav and Soldatović, Ivan A. and Živković-Sandić, Marija and Živković, Slavoljub and Jokanović, Vukoman R.",
year = "2019",
abstract = "Aim: To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology: Twenty-four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t-test) and nonparametric tests (Kruskal–Wallis and Mann–Whitney U-test) depending on data distribution. Results: Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions: This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential. © 2019 International Endodontic Journal. Published by John Wiley & Sons Ltd",
journal = "International Endodontic Journal",
title = "Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis",
pages = "1162-1172",
doi = "10.1111/iej.13105"
}

Paraš, S., Janković, O., Trišić, D., Čolović, B. M., Mitrović‐Ajtić, O., Dekić, R., Soldatović, I. A., Živković-Sandić, M., Živković, S.,& Jokanović, V. R.. (2019). Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis. in International Endodontic Journal, 1162-1172.
https://doi.org/10.1111/iej.13105

Paraš S, Janković O, Trišić D, Čolović BM, Mitrović‐Ajtić O, Dekić R, Soldatović IA, Živković-Sandić M, Živković S, Jokanović VR. Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis. in International Endodontic Journal. 2019;:1162-1172.
doi:10.1111/iej.13105 .

Paraš, Smiljana, Janković, Ognjenka, Trišić, Dijana, Čolović, Božana M., Mitrović‐Ajtić, Olivera, Dekić, Radoslav, Soldatović, Ivan A., Živković-Sandić, Marija, Živković, Slavoljub, Jokanović, Vukoman R., "Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis" in International Endodontic Journal (2019):1162-1172,
https://doi.org/10.1111/iej.13105 . .

TY  - JOUR
AU  - Vasić, Novak
AU  - Glumac, Sofija
AU  - Pejić, Snežana
AU  - Amidžić, L. J.
AU  - Tadić-Latinović, Ljiljana J.
AU  - Dožić, Branko S.
AU  - Hinić, Saša
AU  - Maksimović, Živan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7676
AB  - © 2017 Charles University. All rights reserved. Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) play a complex role in the pathogenesis of atherosclerosis. We compared (1) the histopathological findings in patients with abdominal aortic aneurysms (AAA) and aortoiliac occlusive disease (AOD); (2) the expression of MMP-2/MMP-9 and TIMP-1/TIMP-2 in aortic layers, inflammatory cells and smooth muscle cells (SMCs), aiming to identify the common underlying pathogenic mechanisms of the disease development. Samples were obtained from 30 patients with AAA and 30 with AOD. Aortic histology and immunohistochemistry were performed to evaluate inflammatory changes and MMP and TIMP expression. Thrombosis and ulceration were more frequent in AOD than in AAA. The MMP-9 expression was elevated in all aortic layers of AAA patients and in media/adventitia of AOD patients, mainly followed by lower expression of its inhibitor TIMP-1. Higher MMP-9 expression was also found in SMCs and macrophages of both AAA and AOD specimens, while higher TIMP-1/TIMP-2 were predominantly observed in the lymphocytes and macrophages of the aneurysm. These results showed that both conditions exhibited increased MMP-9 expression; however, the MMP expression pattern differed to some degree between the aneurysms and occlusive disease. The variations in molecular mechanisms underlying dilatative/stenosing disease warrant further investigation.
T2  - Folia Biologica (Czech Republic)
T1  - Expression of matrix metalloproteinases and endogenous inhibitors in abdominal aortic aneurysm and aortoiliac occlusive disease (syndrome leriche)
VL  - 63
IS  - 5-6
SP  - 209
EP  - 216
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7676
ER  - 

@article{
author = "Vasić, Novak and Glumac, Sofija and Pejić, Snežana and Amidžić, L. J. and Tadić-Latinović, Ljiljana J. and Dožić, Branko S. and Hinić, Saša and Maksimović, Živan",
year = "2017",
abstract = "© 2017 Charles University. All rights reserved. Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) play a complex role in the pathogenesis of atherosclerosis. We compared (1) the histopathological findings in patients with abdominal aortic aneurysms (AAA) and aortoiliac occlusive disease (AOD); (2) the expression of MMP-2/MMP-9 and TIMP-1/TIMP-2 in aortic layers, inflammatory cells and smooth muscle cells (SMCs), aiming to identify the common underlying pathogenic mechanisms of the disease development. Samples were obtained from 30 patients with AAA and 30 with AOD. Aortic histology and immunohistochemistry were performed to evaluate inflammatory changes and MMP and TIMP expression. Thrombosis and ulceration were more frequent in AOD than in AAA. The MMP-9 expression was elevated in all aortic layers of AAA patients and in media/adventitia of AOD patients, mainly followed by lower expression of its inhibitor TIMP-1. Higher MMP-9 expression was also found in SMCs and macrophages of both AAA and AOD specimens, while higher TIMP-1/TIMP-2 were predominantly observed in the lymphocytes and macrophages of the aneurysm. These results showed that both conditions exhibited increased MMP-9 expression; however, the MMP expression pattern differed to some degree between the aneurysms and occlusive disease. The variations in molecular mechanisms underlying dilatative/stenosing disease warrant further investigation.",
journal = "Folia Biologica (Czech Republic)",
title = "Expression of matrix metalloproteinases and endogenous inhibitors in abdominal aortic aneurysm and aortoiliac occlusive disease (syndrome leriche)",
volume = "63",
number = "5-6",
pages = "209-216",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7676"
}

Vasić, N., Glumac, S., Pejić, S., Amidžić, L. J., Tadić-Latinović, L. J., Dožić, B. S., Hinić, S.,& Maksimović, Ž.. (2017). Expression of matrix metalloproteinases and endogenous inhibitors in abdominal aortic aneurysm and aortoiliac occlusive disease (syndrome leriche). in Folia Biologica (Czech Republic), 63(5-6), 209-216.
https://hdl.handle.net/21.15107/rcub_vinar_7676

Vasić N, Glumac S, Pejić S, Amidžić LJ, Tadić-Latinović LJ, Dožić BS, Hinić S, Maksimović Ž. Expression of matrix metalloproteinases and endogenous inhibitors in abdominal aortic aneurysm and aortoiliac occlusive disease (syndrome leriche). in Folia Biologica (Czech Republic). 2017;63(5-6):209-216.
https://hdl.handle.net/21.15107/rcub_vinar_7676 .

Vasić, Novak, Glumac, Sofija, Pejić, Snežana, Amidžić, L. J., Tadić-Latinović, Ljiljana J., Dožić, Branko S., Hinić, Saša, Maksimović, Živan, "Expression of matrix metalloproteinases and endogenous inhibitors in abdominal aortic aneurysm and aortoiliac occlusive disease (syndrome leriche)" in Folia Biologica (Czech Republic), 63, no. 5-6 (2017):209-216,
https://hdl.handle.net/21.15107/rcub_vinar_7676 .