Cell Cycle Aberrations and the Impact of Oxidative Stress in Neurodegenerative Processes and Malignant Transformation of the Cell

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Cell Cycle Aberrations and the Impact of Oxidative Stress in Neurodegenerative Processes and Malignant Transformation of the Cell (en)
Аберације ћелијског циклуса и утицај оксидативног стреса на неуродегенеративне процесе и малигну трансформацију ћелије (sr)
Aberacije ćelijskog ciklusa i uticaj oksidativnog stresa na neurodegenerativne procese i malignu transformaciju ćelije (sr_RS)
Authors

Publications

DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases

Essack, Magbubah; Salhi, Adil; Van Neste, Christophe; Raies, Arwa Bin; Tifratene, Faroug; Uludag, Mahmut; Hungler, Arnaud; Zarić, Božidarka; Zafirović, Sonja; Gojobori, Takashi; Isenović, Esma R.; Bajić, Vladan P.

(2020)

TY  - JOUR
AU  - Essack, Magbubah
AU  - Salhi, Adil
AU  - Van Neste, Christophe
AU  - Raies, Arwa Bin
AU  - Tifratene, Faroug
AU  - Uludag, Mahmut
AU  - Hungler, Arnaud
AU  - Zarić, Božidarka
AU  - Zafirović, Sonja
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
AU  - Bajić, Vladan P.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8945
AB  - Normal cellular physiology and biochemical processes require undamaged RNA molecules. However, RNAs are frequently subjected to oxidative damage. Overproduction of reactive oxygen species (ROS) leads to RNA oxidation and disturbs redox (oxidation-reduction reaction) homeostasis. When oxidation damage affects RNA carrying protein-coding information, this may result in the synthesis of aberrant proteins as well as a lower efficiency of translation. Both of these, as well as imbalanced redox homeostasis, may lead to numerous human diseases. The number of studies on the effects of RNA oxidative damage in mammals is increasing by year due to the understanding that this oxidation fundamentally leads to numerous human diseases. To enable researchers in this field to explore information relevant to RNA oxidation and effects on human diseases, we developed DES-ROD, an online knowledgebase that contains processed information from 298,603 relevant documents that consist of PubMed abstracts and PubMed Central full-text articles. The system utilizes concepts/terms from 38 curated thematic dictionaries mapped to the analyzed documents. Researchers can explore enriched concepts, as well as enriched pairs of putatively associated concepts. In this way, one can explore mutual relationships between any combinations of two concepts from used dictionaries. Dictionaries cover a wide range of biomedical topics, such as human genes and proteins, pathways, Gene Ontology categories, mutations, noncoding RNAs, enzymes, toxins, metabolites, and diseases. This makes insights into different facets of the effects of RNA oxidation and the control of this process possible. The usefulness of the DES-ROD system is demonstrated by case studies on some known information, as well as potentially novel information involving RNA oxidation and diseases. DES-ROD is the first knowledgebase based on text and data mining that focused on the exploration of RNA oxidation and human diseases.
T2  - Oxidative Medicine and Cellular Longevity
T1  - DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases
VL  - 2020
SP  - 5904315
DO  - 10.1155/2020/5904315
ER  - 
@article{
author = "Essack, Magbubah and Salhi, Adil and Van Neste, Christophe and Raies, Arwa Bin and Tifratene, Faroug and Uludag, Mahmut and Hungler, Arnaud and Zarić, Božidarka and Zafirović, Sonja and Gojobori, Takashi and Isenović, Esma R. and Bajić, Vladan P.",
year = "2020",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/8945",
abstract = "Normal cellular physiology and biochemical processes require undamaged RNA molecules. However, RNAs are frequently subjected to oxidative damage. Overproduction of reactive oxygen species (ROS) leads to RNA oxidation and disturbs redox (oxidation-reduction reaction) homeostasis. When oxidation damage affects RNA carrying protein-coding information, this may result in the synthesis of aberrant proteins as well as a lower efficiency of translation. Both of these, as well as imbalanced redox homeostasis, may lead to numerous human diseases. The number of studies on the effects of RNA oxidative damage in mammals is increasing by year due to the understanding that this oxidation fundamentally leads to numerous human diseases. To enable researchers in this field to explore information relevant to RNA oxidation and effects on human diseases, we developed DES-ROD, an online knowledgebase that contains processed information from 298,603 relevant documents that consist of PubMed abstracts and PubMed Central full-text articles. The system utilizes concepts/terms from 38 curated thematic dictionaries mapped to the analyzed documents. Researchers can explore enriched concepts, as well as enriched pairs of putatively associated concepts. In this way, one can explore mutual relationships between any combinations of two concepts from used dictionaries. Dictionaries cover a wide range of biomedical topics, such as human genes and proteins, pathways, Gene Ontology categories, mutations, noncoding RNAs, enzymes, toxins, metabolites, and diseases. This makes insights into different facets of the effects of RNA oxidation and the control of this process possible. The usefulness of the DES-ROD system is demonstrated by case studies on some known information, as well as potentially novel information involving RNA oxidation and diseases. DES-ROD is the first knowledgebase based on text and data mining that focused on the exploration of RNA oxidation and human diseases.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases",
volume = "2020",
pages = "5904315",
doi = "10.1155/2020/5904315"
}
Essack, M., Salhi, A., Van Neste, C., Raies, A. B., Tifratene, F., Uludag, M., Hungler, A., Zarić, B., Zafirović, S., Gojobori, T., Isenović, E. R.,& Bajić, V. P. (2020). DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases.
Oxidative Medicine and Cellular Longevity, 2020, 5904315.
https://doi.org/10.1155/2020/5904315
Essack M, Salhi A, Van Neste C, Raies AB, Tifratene F, Uludag M, Hungler A, Zarić B, Zafirović S, Gojobori T, Isenović ER, Bajić VP. DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases. Oxidative Medicine and Cellular Longevity. 2020;2020:5904315
Essack Magbubah, Salhi Adil, Van Neste Christophe, Raies Arwa Bin, Tifratene Faroug, Uludag Mahmut, Hungler Arnaud, Zarić Božidarka, Zafirović Sonja, Gojobori Takashi, Isenović Esma R., Bajić Vladan P., "DES-ROD: Exploring Literature to Develop New Links between RNA Oxidation and Human Diseases" Oxidative Medicine and Cellular Longevity, 2020 (2020):5904315,
https://doi.org/10.1155/2020/5904315 .
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The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”

Bajić, Vladan P.; Essack, Magbubah; Živković, Lada; Stewart, Alan J.; Zafirović, Sonja; Bajić, Vladimir B.; Gojobori, Takashi; Isenović, Esma R.; Spremo-Potparević, Biljana

(2020)

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Essack, Magbubah
AU  - Živković, Lada
AU  - Stewart, Alan J.
AU  - Zafirović, Sonja
AU  - Bajić, Vladimir B.
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8825
AB  - Alzheimer’s disease (AD) is a neurodegenerative disease that affects millions of individuals worldwide and can occur relatively early or later in life. It is well known that genetic components, such as the amyloid precursor protein gene on chromosome 21, are fundamental in early-onset AD (EOAD). To date, however, only the apolipoprotein E4 (ApoE4) gene has been proved to be a genetic risk factor for late-onset AD (LOAD). In recent years, despite the hypothesis that many additional unidentified genes are likely to play a role in AD development, it is surprising that additional gene polymorphisms associated with LOAD have failed to come to light. In this review, we examine the role of X chromosome epigenetics and, based upon GWAS studies, the PCDHX11 gene. Furthermore, we explore other genetic risk factors of AD that involve X-chromosome epigenetics. © Copyright © 2020 Bajic, Essack, Zivkovic, Stewart, Zafirovic, Bajic, Gojobori, Isenovic and Spremo-Potparevic.
T2  - Frontiers in Genetics
T1  - The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”
VL  - 10
DO  - 10.3389/fgene.2019.01368
ER  - 
@article{
author = "Bajić, Vladan P. and Essack, Magbubah and Živković, Lada and Stewart, Alan J. and Zafirović, Sonja and Bajić, Vladimir B. and Gojobori, Takashi and Isenović, Esma R. and Spremo-Potparević, Biljana",
year = "2020",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/8825",
abstract = "Alzheimer’s disease (AD) is a neurodegenerative disease that affects millions of individuals worldwide and can occur relatively early or later in life. It is well known that genetic components, such as the amyloid precursor protein gene on chromosome 21, are fundamental in early-onset AD (EOAD). To date, however, only the apolipoprotein E4 (ApoE4) gene has been proved to be a genetic risk factor for late-onset AD (LOAD). In recent years, despite the hypothesis that many additional unidentified genes are likely to play a role in AD development, it is surprising that additional gene polymorphisms associated with LOAD have failed to come to light. In this review, we examine the role of X chromosome epigenetics and, based upon GWAS studies, the PCDHX11 gene. Furthermore, we explore other genetic risk factors of AD that involve X-chromosome epigenetics. © Copyright © 2020 Bajic, Essack, Zivkovic, Stewart, Zafirovic, Bajic, Gojobori, Isenovic and Spremo-Potparevic.",
journal = "Frontiers in Genetics",
title = "The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”",
volume = "10",
doi = "10.3389/fgene.2019.01368"
}
Bajić, V. P., Essack, M., Živković, L., Stewart, A. J., Zafirović, S., Bajić, V. B., Gojobori, T., Isenović, E. R.,& Spremo-Potparević, B. (2020). The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”.
Frontiers in Genetics, 10.
https://doi.org/10.3389/fgene.2019.01368
Bajić VP, Essack M, Živković L, Stewart AJ, Zafirović S, Bajić VB, Gojobori T, Isenović ER, Spremo-Potparević B. The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”. Frontiers in Genetics. 2020;10
Bajić Vladan P., Essack Magbubah, Živković Lada, Stewart Alan J., Zafirović Sonja, Bajić Vladimir B., Gojobori Takashi, Isenović Esma R., Spremo-Potparević Biljana, "The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”" Frontiers in Genetics, 10 (2020),
https://doi.org/10.3389/fgene.2019.01368 .
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Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy

Obradović, Milan M.; Zafirović, Sonja; Essack, Magbubah; Dimitrov, Jelena; Živković, Lada; Spremo-Potparević, Biljana; Radak, Đorđe J.; Bajić, Vladimir B.; Isenović, Esma R.

(Churchill Livingstone, 2020)

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Essack, Magbubah
AU  - Dimitrov, Jelena
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Radak, Đorđe J.
AU  - Bajić, Vladimir B.
AU  - Isenović, Esma R.
PY  - 2020
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8487
AB  - To remedy carotid artery stenosis and prevent stroke surgical intervention is commonly used, and the gold standard being carotid endarterectomy (CEA). During CEA cerebrovascular hemoglobin oxygen saturation decreases and when this decrease reaches critical levels it leads to cerebral hypoxia that causes neuronal damage. One of the proposed mechanism that affects changes during CEA and contribute to acute brain ischemia (ABI) is oxidative stress. The increased production of reactive oxygen species and reactive nitrogen species during ABI may cause an unregulated inflammatory response and further lead to structural and functional injury of neurons. Antioxidant activity are involved in the protection against neuronal damage after cerebral ischemia. We hypothesized that neuronal injury and poor outcomes in patients undergoing CEA may be results of oxidative stress that disturbed function of antioxidant enzymes and contributed to the DNA damage in lymphocytes. © 2019 The Authors
PB  - Churchill Livingstone
T2  - Medical Hypotheses
T1  - Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy
VL  - 134
SP  - 109419
DO  - 10.1016/j.mehy.2019.109419
ER  - 
@article{
author = "Obradović, Milan M. and Zafirović, Sonja and Essack, Magbubah and Dimitrov, Jelena and Živković, Lada and Spremo-Potparević, Biljana and Radak, Đorđe J. and Bajić, Vladimir B. and Isenović, Esma R.",
year = "2020",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8487",
abstract = "To remedy carotid artery stenosis and prevent stroke surgical intervention is commonly used, and the gold standard being carotid endarterectomy (CEA). During CEA cerebrovascular hemoglobin oxygen saturation decreases and when this decrease reaches critical levels it leads to cerebral hypoxia that causes neuronal damage. One of the proposed mechanism that affects changes during CEA and contribute to acute brain ischemia (ABI) is oxidative stress. The increased production of reactive oxygen species and reactive nitrogen species during ABI may cause an unregulated inflammatory response and further lead to structural and functional injury of neurons. Antioxidant activity are involved in the protection against neuronal damage after cerebral ischemia. We hypothesized that neuronal injury and poor outcomes in patients undergoing CEA may be results of oxidative stress that disturbed function of antioxidant enzymes and contributed to the DNA damage in lymphocytes. © 2019 The Authors",
publisher = "Churchill Livingstone",
journal = "Medical Hypotheses",
title = "Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy",
volume = "134",
pages = "109419",
doi = "10.1016/j.mehy.2019.109419"
}
Obradović, M. M., Zafirović, S., Essack, M., Dimitrov, J., Živković, L., Spremo-Potparević, B., Radak, Đ. J., Bajić, V. B.,& Isenović, E. R. (2020). Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy.
Medical Hypotheses
Churchill Livingstone., 134, 109419.
https://doi.org/10.1016/j.mehy.2019.109419
Obradović MM, Zafirović S, Essack M, Dimitrov J, Živković L, Spremo-Potparević B, Radak ĐJ, Bajić VB, Isenović ER. Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy. Medical Hypotheses. 2020;134:109419
Obradović Milan M., Zafirović Sonja, Essack Magbubah, Dimitrov Jelena, Živković Lada, Spremo-Potparević Biljana, Radak Đorđe J., Bajić Vladimir B., Isenović Esma R., "Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy" Medical Hypotheses, 134 (2020):109419,
https://doi.org/10.1016/j.mehy.2019.109419 .
1

Redox control of vascular biology

Obradović, Milan M.; Essack, Magbubah; Zafirović, Sonja; Sudar-Milovanović, Emina; Bajić, Vladan P.; Van Neste, Christophe; Trpković, Andreja; Stanimirović, Julijana; Bajić, Vladimir B.; Isenović, Esma R.

(2020)

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Essack, Magbubah
AU  - Zafirović, Sonja
AU  - Sudar-Milovanović, Emina
AU  - Bajić, Vladan P.
AU  - Van Neste, Christophe
AU  - Trpković, Andreja
AU  - Stanimirović, Julijana
AU  - Bajić, Vladimir B.
AU  - Isenović, Esma R.
PY  - 2020
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8486
AB  - Redox control is lost when the antioxidant defense system cannot remove abnormally high concentrations of signaling molecules, such as reactive oxygen species (ROS). Chronically elevated levels of ROS cause oxidative stress that may eventually lead to cancer and cardiovascular and neurodegenerative diseases. In this review, we focus on redox effects in the vascular system. We pay close attention to the subcompartments of the vascular system (endothelium, smooth muscle cell layer) and give an overview of how redox changes influence those different compartments. We also review the core aspects of redox biology, cardiovascular physiology, and pathophysiology. Moreover, the topic-specific knowledgebase DES-RedoxVasc was used to develop two case studies, one focused on endothelial cells and the other on the vascular smooth muscle cells, as a starting point to possibly extend our knowledge of redox control in vascular biology. © 2019 The Authors. BioFactors published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology.
T2  - BioFactors
T1  - Redox control of vascular biology
VL  - 46
IS  - 2
SP  - 246
EP  - 262
DO  - 10.1002/biof.1559
ER  - 
@article{
author = "Obradović, Milan M. and Essack, Magbubah and Zafirović, Sonja and Sudar-Milovanović, Emina and Bajić, Vladan P. and Van Neste, Christophe and Trpković, Andreja and Stanimirović, Julijana and Bajić, Vladimir B. and Isenović, Esma R.",
year = "2020",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8486",
abstract = "Redox control is lost when the antioxidant defense system cannot remove abnormally high concentrations of signaling molecules, such as reactive oxygen species (ROS). Chronically elevated levels of ROS cause oxidative stress that may eventually lead to cancer and cardiovascular and neurodegenerative diseases. In this review, we focus on redox effects in the vascular system. We pay close attention to the subcompartments of the vascular system (endothelium, smooth muscle cell layer) and give an overview of how redox changes influence those different compartments. We also review the core aspects of redox biology, cardiovascular physiology, and pathophysiology. Moreover, the topic-specific knowledgebase DES-RedoxVasc was used to develop two case studies, one focused on endothelial cells and the other on the vascular smooth muscle cells, as a starting point to possibly extend our knowledge of redox control in vascular biology. © 2019 The Authors. BioFactors published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology.",
journal = "BioFactors",
title = "Redox control of vascular biology",
volume = "46",
number = "2",
pages = "246-262",
doi = "10.1002/biof.1559"
}
Obradović, M. M., Essack, M., Zafirović, S., Sudar-Milovanović, E., Bajić, V. P., Van Neste, C., Trpković, A., Stanimirović, J., Bajić, V. B.,& Isenović, E. R. (2020). Redox control of vascular biology.
BioFactors, 46(2), 246-262.
https://doi.org/10.1002/biof.1559
Obradović MM, Essack M, Zafirović S, Sudar-Milovanović E, Bajić VP, Van Neste C, Trpković A, Stanimirović J, Bajić VB, Isenović ER. Redox control of vascular biology. BioFactors. 2020;46(2):246-262
Obradović Milan M., Essack Magbubah, Zafirović Sonja, Sudar-Milovanović Emina, Bajić Vladan P., Van Neste Christophe, Trpković Andreja, Stanimirović Julijana, Bajić Vladimir B., Isenović Esma R., "Redox control of vascular biology" BioFactors, 46, no. 2 (2020):246-262,
https://doi.org/10.1002/biof.1559 .
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Evaluation of antioxidant potential of Cordyceps sinensis in vitro

Živković, Lada; Borozan, Sunčica; Bajić, Vladan P.; Đorđević, Stefana; Hristov, Aleksandar; Spremo-Potparević, Biljana

(2019)

TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Bajić, Vladan P.
AU  - Đorđević, Stefana
AU  - Hristov, Aleksandar
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9002
AB  - Objective. Prooxidants and antioxidants affect the oxidative balance at the intracellular level. Oxidative stress is a consequence of the overproduction of prooxidants and is caused by disturbances in the balance of oxidative reduction processes. Non-enzymatic low molecular weight antioxidants can be introduced into the body through food. Cordyceps sinensis (C. sinensis) is a medicinal fungus used in traditional Chinese medicine, with rich content of vitamins, various polysaccharides, and many nucleosides. The aim of this study is to evaluate the antioxidant capacity of the dietary supplement C. sinensis. Methods. The capacity of the hydroxyl radical scavenger activity, the total antioxidant activity of FRAP (Ferric Reducing Antioxidant Power) and the DPPH (2,2-diphenyl-1picrylhydrazyl) scavenger activity were measured. Results. C. sinensis at the tested concentrations of 0.0078-2.00 mg/mL had a pronounced ability to remove hydroxyl radicals with IC50 of 0.5 mg/mL, while at concentrations (0.0078-10.00 mg / mL) it showed a moderate reducing ability. C sinensis showed no ability to remove DPPH radicals. Conclusion. C. sinensis effectively removes hydroxyl radicals, for which the body does not have adequate antioxidant protection, so we can include it in the group of free radical scavengers.
AB  - Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet "skevindžer" aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil) - skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078-2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078-10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.
T2  - Medicinski časopis
T1  - Evaluation of antioxidant potential of Cordyceps sinensis in vitro
T1  - Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro
VL  - 53
IS  - 4
SP  - 129
EP  - 134
DO  - 10.5937/mckg53-24450
ER  - 
@article{
author = "Živković, Lada and Borozan, Sunčica and Bajić, Vladan P. and Đorđević, Stefana and Hristov, Aleksandar and Spremo-Potparević, Biljana",
year = "2019",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/9002",
abstract = "Objective. Prooxidants and antioxidants affect the oxidative balance at the intracellular level. Oxidative stress is a consequence of the overproduction of prooxidants and is caused by disturbances in the balance of oxidative reduction processes. Non-enzymatic low molecular weight antioxidants can be introduced into the body through food. Cordyceps sinensis (C. sinensis) is a medicinal fungus used in traditional Chinese medicine, with rich content of vitamins, various polysaccharides, and many nucleosides. The aim of this study is to evaluate the antioxidant capacity of the dietary supplement C. sinensis. Methods. The capacity of the hydroxyl radical scavenger activity, the total antioxidant activity of FRAP (Ferric Reducing Antioxidant Power) and the DPPH (2,2-diphenyl-1picrylhydrazyl) scavenger activity were measured. Results. C. sinensis at the tested concentrations of 0.0078-2.00 mg/mL had a pronounced ability to remove hydroxyl radicals with IC50 of 0.5 mg/mL, while at concentrations (0.0078-10.00 mg / mL) it showed a moderate reducing ability. C sinensis showed no ability to remove DPPH radicals. Conclusion. C. sinensis effectively removes hydroxyl radicals, for which the body does not have adequate antioxidant protection, so we can include it in the group of free radical scavengers., Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet "skevindžer" aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil) - skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078-2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078-10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.",
journal = "Medicinski časopis",
title = "Evaluation of antioxidant potential of Cordyceps sinensis in vitro, Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro",
volume = "53",
number = "4",
pages = "129-134",
doi = "10.5937/mckg53-24450"
}
Živković, L., Borozan, S., Bajić, V. P., Đorđević, S., Hristov, A.,& Spremo-Potparević, B. (2019). Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro.
Medicinski časopis, 53(4), 129-134.
https://doi.org/10.5937/mckg53-24450
Živković L, Borozan S, Bajić VP, Đorđević S, Hristov A, Spremo-Potparević B. Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro. Medicinski časopis. 2019;53(4):129-134
Živković Lada, Borozan Sunčica, Bajić Vladan P., Đorđević Stefana, Hristov Aleksandar, Spremo-Potparević Biljana, "Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro" Medicinski časopis, 53, no. 4 (2019):129-134,
https://doi.org/10.5937/mckg53-24450 .

Literature-Based Enrichment Insights into Redox Control of Vascular Biology

Essack, Magbubah; Salhi, Adil; Stanimirović, Julijana; Tifratene, Faroug; Bin Raies, Arwa; Hungler, Arnaud; Uludag, Mahmut; Van Neste, Christophe; Trpković, Andreja; Bajić, Vladan P.; Bajić, Vladimir B.; Isenović, Esma R.

(2019)

TY  - JOUR
AU  - Essack, Magbubah
AU  - Salhi, Adil
AU  - Stanimirović, Julijana
AU  - Tifratene, Faroug
AU  - Bin Raies, Arwa
AU  - Hungler, Arnaud
AU  - Uludag, Mahmut
AU  - Van Neste, Christophe
AU  - Trpković, Andreja
AU  - Bajić, Vladan P.
AU  - Bajić, Vladimir B.
AU  - Isenović, Esma R.
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8389
AB  - In cellular physiology and signaling, reactive oxygen species (ROS) play one of the most critical roles. ROS overproduction leads to cellular oxidative stress. This may lead to an irrecoverable imbalance of redox (oxidation-reduction reaction) function that deregulates redox homeostasis, which itself could lead to several diseases including neurodegenerative disease, cardiovascular disease, and cancers. In this study, we focus on the redox effects related to vascular systems in mammals. To support research in this domain, we developed an online knowledge base, DES-RedoxVasc, which enables exploration of information contained in the biomedical scientific literature. The DES-RedoxVasc system analyzed 233399 documents consisting of PubMed abstracts and PubMed Central full-text articles related to different aspects of redox biology in vascular systems. It allows researchers to explore enriched concepts from 28 curated thematic dictionaries, as well as literature-derived potential associations of pairs of such enriched concepts, where associations themselves are statistically enriched. For example, the system allows exploration of associations of pathways, diseases, mutations, genes/proteins, miRNAs, long ncRNAs, toxins, drugs, biological processes, molecular functions, etc. that allow for insights about different aspects of redox effects and control of processes related to the vascular system. Moreover, we deliver case studies about some existing or possibly novel knowledge regarding redox of vascular biology demonstrating the usefulness of DES-RedoxVasc. DES-RedoxVasc is the first compiled knowledge base using text mining for the exploration of this topic.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Literature-Based Enrichment Insights into Redox Control of Vascular Biology
VL  - 2019
SP  - 1769437
DO  - 10.1155/2019/1769437
ER  - 
@article{
author = "Essack, Magbubah and Salhi, Adil and Stanimirović, Julijana and Tifratene, Faroug and Bin Raies, Arwa and Hungler, Arnaud and Uludag, Mahmut and Van Neste, Christophe and Trpković, Andreja and Bajić, Vladan P. and Bajić, Vladimir B. and Isenović, Esma R.",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8389",
abstract = "In cellular physiology and signaling, reactive oxygen species (ROS) play one of the most critical roles. ROS overproduction leads to cellular oxidative stress. This may lead to an irrecoverable imbalance of redox (oxidation-reduction reaction) function that deregulates redox homeostasis, which itself could lead to several diseases including neurodegenerative disease, cardiovascular disease, and cancers. In this study, we focus on the redox effects related to vascular systems in mammals. To support research in this domain, we developed an online knowledge base, DES-RedoxVasc, which enables exploration of information contained in the biomedical scientific literature. The DES-RedoxVasc system analyzed 233399 documents consisting of PubMed abstracts and PubMed Central full-text articles related to different aspects of redox biology in vascular systems. It allows researchers to explore enriched concepts from 28 curated thematic dictionaries, as well as literature-derived potential associations of pairs of such enriched concepts, where associations themselves are statistically enriched. For example, the system allows exploration of associations of pathways, diseases, mutations, genes/proteins, miRNAs, long ncRNAs, toxins, drugs, biological processes, molecular functions, etc. that allow for insights about different aspects of redox effects and control of processes related to the vascular system. Moreover, we deliver case studies about some existing or possibly novel knowledge regarding redox of vascular biology demonstrating the usefulness of DES-RedoxVasc. DES-RedoxVasc is the first compiled knowledge base using text mining for the exploration of this topic.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Literature-Based Enrichment Insights into Redox Control of Vascular Biology",
volume = "2019",
pages = "1769437",
doi = "10.1155/2019/1769437"
}
Essack, M., Salhi, A., Stanimirović, J., Tifratene, F., Bin Raies, A., Hungler, A., Uludag, M., Van Neste, C., Trpković, A., Bajić, V. P., Bajić, V. B.,& Isenović, E. R. (2019). Literature-Based Enrichment Insights into Redox Control of Vascular Biology.
Oxidative Medicine and Cellular Longevity, 2019, 1769437.
https://doi.org/10.1155/2019/1769437
Essack M, Salhi A, Stanimirović J, Tifratene F, Bin Raies A, Hungler A, Uludag M, Van Neste C, Trpković A, Bajić VP, Bajić VB, Isenović ER. Literature-Based Enrichment Insights into Redox Control of Vascular Biology. Oxidative Medicine and Cellular Longevity. 2019;2019:1769437
Essack Magbubah, Salhi Adil, Stanimirović Julijana, Tifratene Faroug, Bin Raies Arwa, Hungler Arnaud, Uludag Mahmut, Van Neste Christophe, Trpković Andreja, Bajić Vladan P., Bajić Vladimir B., Isenović Esma R., "Literature-Based Enrichment Insights into Redox Control of Vascular Biology" Oxidative Medicine and Cellular Longevity, 2019 (2019):1769437,
https://doi.org/10.1155/2019/1769437 .
4
1
3

Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease

Bajić, Vladan P.; Van Neste, Christophe; Obradović, Milan M.; Zafirović, Sonja; Radak, Đorđe J.; Bajić, Vladimir B.; Essack, Magbubah; Isenović, Esma R.

(2019)

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Van Neste, Christophe
AU  - Obradović, Milan M.
AU  - Zafirović, Sonja
AU  - Radak, Đorđe J.
AU  - Bajić, Vladimir B.
AU  - Essack, Magbubah
AU  - Isenović, Esma R.
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8375
AB  - More people die from cardiovascular diseases (CVD) than from any other cause. Cardiovascular complications are thought to arise from enhanced levels of free radicals causing impaired “redox homeostasis,” which represents the interplay between oxidative stress (OS) and reductive stress (RS). In this review, we compile several experimental research findings that show sustained shifts towards OS will alter the homeostatic redox mechanism to cause cardiovascular complications, as well as findings that show a prolonged antioxidant state or RS can similarly lead to such cardiovascular complications. This experimental evidence is specifically focused on the role of glutathione, the most abundant antioxidant in the heart, in a redox homeostatic mechanism that has been shifted towards OS or RS. This may lead to impairment of cellular signaling mechanisms and elevated pools of proteotoxicity associated with cardiac dysfunction.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease
VL  - 2019
SP  - 5028181
DO  - 10.1155/2019/5028181
ER  - 
@article{
author = "Bajić, Vladan P. and Van Neste, Christophe and Obradović, Milan M. and Zafirović, Sonja and Radak, Đorđe J. and Bajić, Vladimir B. and Essack, Magbubah and Isenović, Esma R.",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8375",
abstract = "More people die from cardiovascular diseases (CVD) than from any other cause. Cardiovascular complications are thought to arise from enhanced levels of free radicals causing impaired “redox homeostasis,” which represents the interplay between oxidative stress (OS) and reductive stress (RS). In this review, we compile several experimental research findings that show sustained shifts towards OS will alter the homeostatic redox mechanism to cause cardiovascular complications, as well as findings that show a prolonged antioxidant state or RS can similarly lead to such cardiovascular complications. This experimental evidence is specifically focused on the role of glutathione, the most abundant antioxidant in the heart, in a redox homeostatic mechanism that has been shifted towards OS or RS. This may lead to impairment of cellular signaling mechanisms and elevated pools of proteotoxicity associated with cardiac dysfunction.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease",
volume = "2019",
pages = "5028181",
doi = "10.1155/2019/5028181"
}
Bajić, V. P., Van Neste, C., Obradović, M. M., Zafirović, S., Radak, Đ. J., Bajić, V. B., Essack, M.,& Isenović, E. R. (2019). Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease.
Oxidative Medicine and Cellular Longevity, 2019, 5028181.
https://doi.org/10.1155/2019/5028181
Bajić VP, Van Neste C, Obradović MM, Zafirović S, Radak ĐJ, Bajić VB, Essack M, Isenović ER. Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease. Oxidative Medicine and Cellular Longevity. 2019;2019:5028181
Bajić Vladan P., Van Neste Christophe, Obradović Milan M., Zafirović Sonja, Radak Đorđe J., Bajić Vladimir B., Essack Magbubah, Isenović Esma R., "Glutathione “Redox Homeostasis” and Its Relation to Cardiovascular Disease" Oxidative Medicine and Cellular Longevity, 2019 (2019):5028181,
https://doi.org/10.1155/2019/5028181 .
1
26
19
24

Drug Delivery Systems for Diabetes Treatment

Zarić, Božidarka; Obradović, Milan M.; Sudar-Milovanović, Emina; Nedeljković, Jovan; Lazić, Vesna M.; Isenović, Esma R.

(2019)

TY  - JOUR
AU  - Zarić, Božidarka
AU  - Obradović, Milan M.
AU  - Sudar-Milovanović, Emina
AU  - Nedeljković, Jovan
AU  - Lazić, Vesna M.
AU  - Isenović, Esma R.
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8378
AB  - Background: Insulin is essential for the treatment of Type 1 diabetes mellitus (T1DM) and is necessary in numerous cases of Type 2 diabetes mellitus (T2DM). Prolonged administration of anti-diabetic therapy is necessary for the maintenance of the normal glucose levels and thereby preventing vascular complications. A better understanding of the disease per se and the technological progress contribute to the development of new approaches with the aim to achieve better glycemic control. Objective: Current therapies for DM are faced with some challenges. The purpose of this review is to analyze in detail the current trends for insulin delivery systems for diabetes treatment. Results: Contemporary ways have been proposed for the management of both types of diabetes by adequate application of drug via subcutaneous, buccal, oral, ocular, nasal, rectal and pulmonary ways. Development of improved oral administration of insulin is beneficial regarding mimicking physiological pathway of insulin and minimizing the discomfort of the patient. Various nanoparticle carriers for oral and other ways of insulin delivery are currently being developed. Engineered specific properties of nanoparticles (NP): controlling toxicity of NP, stability and drug release, can allow delivery of higher concentration of the drug to the desired location. Conclusions: The successful development of any drug delivery system relies on solving three important issues: toxicity of nanoparticles, stability of nanoparticles, and desired drug release rate at targeted sites. The main goals of future investigations are to improve the existing therapies by pharmacokinetic modifications, development of a fully automatized system to mimic insulin delivery by the pancreas and reduce invasiveness during admission. © 2019 Bentham Science Publishers.
T2  - Current Pharmaceutical Design
T1  - Drug Delivery Systems for Diabetes Treatment
VL  - 25
IS  - 2
SP  - 166
EP  - 173
DO  - 10.2174/1381612825666190306153838
ER  - 
@article{
author = "Zarić, Božidarka and Obradović, Milan M. and Sudar-Milovanović, Emina and Nedeljković, Jovan and Lazić, Vesna M. and Isenović, Esma R.",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8378",
abstract = "Background: Insulin is essential for the treatment of Type 1 diabetes mellitus (T1DM) and is necessary in numerous cases of Type 2 diabetes mellitus (T2DM). Prolonged administration of anti-diabetic therapy is necessary for the maintenance of the normal glucose levels and thereby preventing vascular complications. A better understanding of the disease per se and the technological progress contribute to the development of new approaches with the aim to achieve better glycemic control. Objective: Current therapies for DM are faced with some challenges. The purpose of this review is to analyze in detail the current trends for insulin delivery systems for diabetes treatment. Results: Contemporary ways have been proposed for the management of both types of diabetes by adequate application of drug via subcutaneous, buccal, oral, ocular, nasal, rectal and pulmonary ways. Development of improved oral administration of insulin is beneficial regarding mimicking physiological pathway of insulin and minimizing the discomfort of the patient. Various nanoparticle carriers for oral and other ways of insulin delivery are currently being developed. Engineered specific properties of nanoparticles (NP): controlling toxicity of NP, stability and drug release, can allow delivery of higher concentration of the drug to the desired location. Conclusions: The successful development of any drug delivery system relies on solving three important issues: toxicity of nanoparticles, stability of nanoparticles, and desired drug release rate at targeted sites. The main goals of future investigations are to improve the existing therapies by pharmacokinetic modifications, development of a fully automatized system to mimic insulin delivery by the pancreas and reduce invasiveness during admission. © 2019 Bentham Science Publishers.",
journal = "Current Pharmaceutical Design",
title = "Drug Delivery Systems for Diabetes Treatment",
volume = "25",
number = "2",
pages = "166-173",
doi = "10.2174/1381612825666190306153838"
}
Zarić, B., Obradović, M. M., Sudar-Milovanović, E., Nedeljković, J., Lazić, V. M.,& Isenović, E. R. (2019). Drug Delivery Systems for Diabetes Treatment.
Current Pharmaceutical Design, 25(2), 166-173.
https://doi.org/10.2174/1381612825666190306153838
Zarić B, Obradović MM, Sudar-Milovanović E, Nedeljković J, Lazić VM, Isenović ER. Drug Delivery Systems for Diabetes Treatment. Current Pharmaceutical Design. 2019;25(2):166-173
Zarić Božidarka, Obradović Milan M., Sudar-Milovanović Emina, Nedeljković Jovan, Lazić Vesna M., Isenović Esma R., "Drug Delivery Systems for Diabetes Treatment" Current Pharmaceutical Design, 25, no. 2 (2019):166-173,
https://doi.org/10.2174/1381612825666190306153838 .
7
5
6

Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells

Živković, Lada; Bajić, Vladan P.; Topalović, Dijana; Bruić, Marija; Spremo-Potparević, Biljana

(2019)

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan P.
AU  - Topalović, Dijana
AU  - Bruić, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8666
AB  - The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μ g/mL, 500 μ g/mL and 1000 μ g/mL) and dihydroquercetin (100 μ g/mL, 250 μ g/mL and 500 μ g/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H 2 O 2 -) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H 2 O 2 -induced damage on DNA in cells at all tested concentrations, with a statistical significance ( p < 0.05 ), whereas Biochaga at the dose of 500 μ g/mL in combination with dihydroquercetin 500 μ g/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro .
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells
VL  - 2019
SP  - 5039372
DO  - 10.1155/2019/5039372
ER  - 
@article{
author = "Živković, Lada and Bajić, Vladan P. and Topalović, Dijana and Bruić, Marija and Spremo-Potparević, Biljana",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8666",
abstract = "The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μ g/mL, 500 μ g/mL and 1000 μ g/mL) and dihydroquercetin (100 μ g/mL, 250 μ g/mL and 500 μ g/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H 2 O 2 -) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H 2 O 2 -induced damage on DNA in cells at all tested concentrations, with a statistical significance ( p < 0.05 ), whereas Biochaga at the dose of 500 μ g/mL in combination with dihydroquercetin 500 μ g/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro .",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells",
volume = "2019",
pages = "5039372",
doi = "10.1155/2019/5039372"
}
Živković, L., Bajić, V. P., Topalović, D., Bruić, M.,& Spremo-Potparević, B. (2019). Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells.
Oxidative Medicine and Cellular Longevity, 2019, 5039372.
https://doi.org/10.1155/2019/5039372
Živković L, Bajić VP, Topalović D, Bruić M, Spremo-Potparević B. Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells. Oxidative Medicine and Cellular Longevity. 2019;2019:5039372
Živković Lada, Bajić Vladan P., Topalović Dijana, Bruić Marija, Spremo-Potparević Biljana, "Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells" Oxidative Medicine and Cellular Longevity, 2019 (2019):5039372,
https://doi.org/10.1155/2019/5039372 .
3
1
2

Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro

Živković, Lada; Bajić, Vladan P.; Dekanski, Dragana; Čabarkapa-Pirković, Andrea; Giampieri, Francesca; Gasparrini, Massimiliano; Mazzoni, Luca; Spremo-Potparević, Biljana

(2018)

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan P.
AU  - Dekanski, Dragana
AU  - Čabarkapa-Pirković, Andrea
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Mazzoni, Luca
AU  - Spremo-Potparević, Biljana
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S027869151830334X
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7819
AB  - Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25–1000 μg/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p < 0.001), where concentrations of 25 and 100 μg/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.
T2  - Food and Chemical Toxicology
T1  - Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro
VL  - 119
SP  - 61
EP  - 65
DO  - 10.1016/j.fct.2018.05.034
ER  - 
@article{
author = "Živković, Lada and Bajić, Vladan P. and Dekanski, Dragana and Čabarkapa-Pirković, Andrea and Giampieri, Francesca and Gasparrini, Massimiliano and Mazzoni, Luca and Spremo-Potparević, Biljana",
year = "2018",
url = "https://linkinghub.elsevier.com/retrieve/pii/S027869151830334X, http://vinar.vin.bg.ac.rs/handle/123456789/7819",
abstract = "Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25–1000 μg/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p < 0.001), where concentrations of 25 and 100 μg/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.",
journal = "Food and Chemical Toxicology",
title = "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro",
volume = "119",
pages = "61-65",
doi = "10.1016/j.fct.2018.05.034"
}
Živković, L., Bajić, V. P., Dekanski, D., Čabarkapa-Pirković, A., Giampieri, F., Gasparrini, M., Mazzoni, L.,& Spremo-Potparević, B. (2018). Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro.
Food and Chemical Toxicology, 119, 61-65.
https://doi.org/10.1016/j.fct.2018.05.034
Živković L, Bajić VP, Dekanski D, Čabarkapa-Pirković A, Giampieri F, Gasparrini M, Mazzoni L, Spremo-Potparević B. Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. Food and Chemical Toxicology. 2018;119:61-65
Živković Lada, Bajić Vladan P., Dekanski Dragana, Čabarkapa-Pirković Andrea, Giampieri Francesca, Gasparrini Massimiliano, Mazzoni Luca, Spremo-Potparević Biljana, "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro" Food and Chemical Toxicology, 119 (2018):61-65,
https://doi.org/10.1016/j.fct.2018.05.034 .
8
5
7

Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid

Dekanski, Dragana; Spremo-Potparević, Biljana; Bajić, Vladan P.; Živković, Lada; Topalović, Dijana; Sredojević, Dušan; Lazić, Vesna M.; Nedeljković, Jovan

(2018)

TY  - JOUR
AU  - Dekanski, Dragana
AU  - Spremo-Potparević, Biljana
AU  - Bajić, Vladan P.
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Sredojević, Dušan
AU  - Lazić, Vesna M.
AU  - Nedeljković, Jovan
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0278691518301388
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7790
AB  - The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.
T2  - Food and Chemical Toxicology
T1  - Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid
VL  - 115
SP  - 42
EP  - 48
DO  - 10.1016/j.fct.2018.02.064
ER  - 
@article{
author = "Dekanski, Dragana and Spremo-Potparević, Biljana and Bajić, Vladan P. and Živković, Lada and Topalović, Dijana and Sredojević, Dušan and Lazić, Vesna M. and Nedeljković, Jovan",
year = "2018",
url = "https://linkinghub.elsevier.com/retrieve/pii/S0278691518301388, http://vinar.vin.bg.ac.rs/handle/123456789/7790",
abstract = "The acute toxicity of surface-modified TiO2 nanoparticles (NPs) with caffeic acid (CA) was compared with those of its separate constituents (free CA and bare TiO2 NPs) upon their oral administration in laboratory mice. Prior to in vivo experiments, the interfacial charge transfer (ICT) complex between surface Ti atoms and CA is thoroughly characterized. Composition and stability constants of ICT complex were determined using Job's method and Banesi-Hildebrand analysis, respectively. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). Acute toxicity signs, including biochemical alterations and extensive histopathological changes in the liver tissue of mice were detected 14 days after oral administration of bare TiO2 NPs. However, the clinical signs of toxicity, the fractional contribution of organs, biochemical parameters of liver and kidney function, and histopathological changes in liver upon treatment with surface-modified TiO2 NPs with CA were not observed. Also, the genotoxic potential of the ICT complex and its constituents were evaluated in leukocytes of whole blood cells in vivo by comet assay. Both, bare and surface-modified TiO2 NPs did not display DNA damaging effect in time frame of 24 h upon their oral administration in mice.",
journal = "Food and Chemical Toxicology",
title = "Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid",
volume = "115",
pages = "42-48",
doi = "10.1016/j.fct.2018.02.064"
}
Dekanski, D., Spremo-Potparević, B., Bajić, V. P., Živković, L., Topalović, D., Sredojević, D., Lazić, V. M.,& Nedeljković, J. (2018). Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid.
Food and Chemical Toxicology, 115, 42-48.
https://doi.org/10.1016/j.fct.2018.02.064
Dekanski D, Spremo-Potparević B, Bajić VP, Živković L, Topalović D, Sredojević D, Lazić VM, Nedeljković J. Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid. Food and Chemical Toxicology. 2018;115:42-48
Dekanski Dragana, Spremo-Potparević Biljana, Bajić Vladan P., Živković Lada, Topalović Dijana, Sredojević Dušan, Lazić Vesna M., Nedeljković Jovan, "Acute toxicity study in mice of orally administrated TiO 2 nanoparticles functionalized with caffeic acid" Food and Chemical Toxicology, 115 (2018):42-48,
https://doi.org/10.1016/j.fct.2018.02.064 .
14
11
15

Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres

Živković, Lada; Akar, Banu; Roux, Brianna M.; Spremo-Potparević, Biljana; Bajić, Vladan P.; Brey, Eric M.

(2017)

TY  - JOUR
AU  - Živković, Lada
AU  - Akar, Banu
AU  - Roux, Brianna M.
AU  - Spremo-Potparević, Biljana
AU  - Bajić, Vladan P.
AU  - Brey, Eric M.
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1324
AB  - Poly (lactic-co-glycolic acid) (PLGA)-based materials are widely investigated for drug delivery and tissue engineering applications. Despite their popularity the genotoxic potential of PLGA has not been investigated. In this study, the comet assay, a sensitive assay for DNA damage, was used to evaluate potential genotoxicity in model cell types exposed to PLGA microspheres. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cells were exposed to PLGA microspheres (0.4-6mg/mL) and DNA damage assessed at 24h, 4days, and 7days. DNA damage was not identified after 24h. However, after 4 and 7 days of exposure to 2 and 6mg/mL of PLGA microspheres a significant elevation of DNA damage in both cell types was observed. The PLGA microspheres did not exhibit any cytotoxic effects on the cells under the conditions tested. Our results suggest that PLGA may have a genotoxic effect on cells. A broader investigation of the PLGA genotoxic profile in biological systems is needed. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 284-291, 2017.
T2  - Journal of Biomedical Materials Research. Part A
T1  - Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres
VL  - 105
IS  - 1
SP  - 284
EP  - 291
DO  - 10.1002/jbm.a.35849
ER  - 
@article{
author = "Živković, Lada and Akar, Banu and Roux, Brianna M. and Spremo-Potparević, Biljana and Bajić, Vladan P. and Brey, Eric M.",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1324",
abstract = "Poly (lactic-co-glycolic acid) (PLGA)-based materials are widely investigated for drug delivery and tissue engineering applications. Despite their popularity the genotoxic potential of PLGA has not been investigated. In this study, the comet assay, a sensitive assay for DNA damage, was used to evaluate potential genotoxicity in model cell types exposed to PLGA microspheres. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cells were exposed to PLGA microspheres (0.4-6mg/mL) and DNA damage assessed at 24h, 4days, and 7days. DNA damage was not identified after 24h. However, after 4 and 7 days of exposure to 2 and 6mg/mL of PLGA microspheres a significant elevation of DNA damage in both cell types was observed. The PLGA microspheres did not exhibit any cytotoxic effects on the cells under the conditions tested. Our results suggest that PLGA may have a genotoxic effect on cells. A broader investigation of the PLGA genotoxic profile in biological systems is needed. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 284-291, 2017.",
journal = "Journal of Biomedical Materials Research. Part A",
title = "Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres",
volume = "105",
number = "1",
pages = "284-291",
doi = "10.1002/jbm.a.35849"
}
Živković, L., Akar, B., Roux, B. M., Spremo-Potparević, B., Bajić, V. P.,& Brey, E. M. (2017). Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres.
Journal of Biomedical Materials Research. Part A, 105(1), 284-291.
https://doi.org/10.1002/jbm.a.35849
Živković L, Akar B, Roux BM, Spremo-Potparević B, Bajić VP, Brey EM. Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres. Journal of Biomedical Materials Research. Part A. 2017;105(1):284-291
Živković Lada, Akar Banu, Roux Brianna M., Spremo-Potparević Biljana, Bajić Vladan P., Brey Eric M., "Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres" Journal of Biomedical Materials Research. Part A, 105, no. 1 (2017):284-291,
https://doi.org/10.1002/jbm.a.35849 .
1
3
3
4

DES-ncRNA: A knowledgebase for exploring information about human micro and long noncoding RNAs based on literature-mining

Salhi, Adil; Essack, Magbubah; Alam, Tanvir; Bajić, Vladan P.; Ma, Lina; Radovanovic, Aleksandar; Marchand, Benoit; Schmeier, Sebastian; Zhang, Zhang; Bajić, Vladimir B.

(2017)

TY  - JOUR
AU  - Salhi, Adil
AU  - Essack, Magbubah
AU  - Alam, Tanvir
AU  - Bajić, Vladan P.
AU  - Ma, Lina
AU  - Radovanovic, Aleksandar
AU  - Marchand, Benoit
AU  - Schmeier, Sebastian
AU  - Zhang, Zhang
AU  - Bajić, Vladimir B.
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1675
AB  - Noncoding RNAs (ncRNAs), particularly microRNAs (miRNAs) and long ncRNAs (lncRNAs), are important players in diseases and emerge as novel drug targets. Thus, unraveling the relationships between ncRNAs and other biomedical entities in cells are critical for better understanding ncRNA roles that may eventually help develop their use in medicine. To support ncRNA research and facilitate retrieval of relevant information regarding miRNAs and lncRNAs from the plethora of published ncRNA-related research, we developed DES-ncRNA (www.cbrc.kaust.edu.sa/des_ncrna). DES-ncRNA is a knowledgebase containing text- and data-mined information from public scientific literature and other public resources. Exploration of mined information is enabled through terms and pairs of terms from 19 topic-specific dictionaries including, for example, antibiotics, toxins, drugs, enzymes, mutations, pathways, human genes and proteins, drug indications and side effects, mutations, diseases, etc. DES-ncRNA contains approximately 878,000 associations of terms from these dictionaries of which 36,222 (5,373) are with regards to miRNAs (lncRNAs). We provide several ways to explore information regarding ncRNAs to users including controlled generation of association networks as well as hypotheses generation. We show an example how DES-ncRNA can aid research on Alzheimer disease and suggest potential therapeutic role for Fasudil. DES-ncRNA is a powerful tool that can be used on its own or as a complement to the existing resources, to support research in human ncRNA. To our knowledge, this is the only knowledgebase dedicated to human miRNAs and lncRNAs derived primarily through literature-mining enabling exploration of a broad spectrum of associated biomedical entities, not paralleled by any other resource.
T2  - RNA Biology
T1  - DES-ncRNA: A knowledgebase for exploring information about human micro and long noncoding RNAs based on literature-mining
VL  - 14
IS  - 7
SP  - 963
EP  - 971
DO  - 10.1080/15476286.2017.1312243
ER  - 
@article{
author = "Salhi, Adil and Essack, Magbubah and Alam, Tanvir and Bajić, Vladan P. and Ma, Lina and Radovanovic, Aleksandar and Marchand, Benoit and Schmeier, Sebastian and Zhang, Zhang and Bajić, Vladimir B.",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1675",
abstract = "Noncoding RNAs (ncRNAs), particularly microRNAs (miRNAs) and long ncRNAs (lncRNAs), are important players in diseases and emerge as novel drug targets. Thus, unraveling the relationships between ncRNAs and other biomedical entities in cells are critical for better understanding ncRNA roles that may eventually help develop their use in medicine. To support ncRNA research and facilitate retrieval of relevant information regarding miRNAs and lncRNAs from the plethora of published ncRNA-related research, we developed DES-ncRNA (www.cbrc.kaust.edu.sa/des_ncrna). DES-ncRNA is a knowledgebase containing text- and data-mined information from public scientific literature and other public resources. Exploration of mined information is enabled through terms and pairs of terms from 19 topic-specific dictionaries including, for example, antibiotics, toxins, drugs, enzymes, mutations, pathways, human genes and proteins, drug indications and side effects, mutations, diseases, etc. DES-ncRNA contains approximately 878,000 associations of terms from these dictionaries of which 36,222 (5,373) are with regards to miRNAs (lncRNAs). We provide several ways to explore information regarding ncRNAs to users including controlled generation of association networks as well as hypotheses generation. We show an example how DES-ncRNA can aid research on Alzheimer disease and suggest potential therapeutic role for Fasudil. DES-ncRNA is a powerful tool that can be used on its own or as a complement to the existing resources, to support research in human ncRNA. To our knowledge, this is the only knowledgebase dedicated to human miRNAs and lncRNAs derived primarily through literature-mining enabling exploration of a broad spectrum of associated biomedical entities, not paralleled by any other resource.",
journal = "RNA Biology",
title = "DES-ncRNA: A knowledgebase for exploring information about human micro and long noncoding RNAs based on literature-mining",
volume = "14",
number = "7",
pages = "963-971",
doi = "10.1080/15476286.2017.1312243"
}
Salhi, A., Essack, M., Alam, T., Bajić, V. P., Ma, L., Radovanovic, A., Marchand, B., Schmeier, S., Zhang, Z.,& Bajić, V. B. (2017). DES-ncRNA: A knowledgebase for exploring information about human micro and long noncoding RNAs based on literature-mining.
RNA Biology, 14(7), 963-971.
https://doi.org/10.1080/15476286.2017.1312243
Salhi A, Essack M, Alam T, Bajić VP, Ma L, Radovanovic A, Marchand B, Schmeier S, Zhang Z, Bajić VB. DES-ncRNA: A knowledgebase for exploring information about human micro and long noncoding RNAs based on literature-mining. RNA Biology. 2017;14(7):963-971
Salhi Adil, Essack Magbubah, Alam Tanvir, Bajić Vladan P., Ma Lina, Radovanovic Aleksandar, Marchand Benoit, Schmeier Sebastian, Zhang Zhang, Bajić Vladimir B., "DES-ncRNA: A knowledgebase for exploring information about human micro and long noncoding RNAs based on literature-mining" RNA Biology, 14, no. 7 (2017):963-971,
https://doi.org/10.1080/15476286.2017.1312243 .
9
15
8
10

Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro

Bajić, Vladan P.; Spremo-Potparević, Biljana; Živković, Lada; Cabarkapa, Andrea; Kotur-Stevuljevic, Jelena; Isenović, Esma R.; Sredojević, Dušan; Vukoje, Ivana D.; Lazić, Vesna M.; Ahrenkiel, Scott Phillip; Nedeljković, Jovan

(2017)

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Cabarkapa, Andrea
AU  - Kotur-Stevuljevic, Jelena
AU  - Isenović, Esma R.
AU  - Sredojević, Dušan
AU  - Vukoje, Ivana D.
AU  - Lazić, Vesna M.
AU  - Ahrenkiel, Scott Phillip
AU  - Nedeljković, Jovan
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1607
AB  - The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Jobs method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro
VL  - 155
SP  - 323
EP  - 331
DO  - 10.1016/j.colsurfb.2017.04.032
ER  - 
@article{
author = "Bajić, Vladan P. and Spremo-Potparević, Biljana and Živković, Lada and Cabarkapa, Andrea and Kotur-Stevuljevic, Jelena and Isenović, Esma R. and Sredojević, Dušan and Vukoje, Ivana D. and Lazić, Vesna M. and Ahrenkiel, Scott Phillip and Nedeljković, Jovan",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1607",
abstract = "The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Jobs method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro",
volume = "155",
pages = "323-331",
doi = "10.1016/j.colsurfb.2017.04.032"
}
Bajić, V. P., Spremo-Potparević, B., Živković, L., Cabarkapa, A., Kotur-Stevuljevic, J., Isenović, E. R., Sredojević, D., Vukoje, I. D., Lazić, V. M., Ahrenkiel, S. P.,& Nedeljković, J. (2017). Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro.
Colloids and Surfaces. B: Biointerfaces, 155, 323-331.
https://doi.org/10.1016/j.colsurfb.2017.04.032
Bajić VP, Spremo-Potparević B, Živković L, Cabarkapa A, Kotur-Stevuljevic J, Isenović ER, Sredojević D, Vukoje ID, Lazić VM, Ahrenkiel SP, Nedeljković J. Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro. Colloids and Surfaces. B: Biointerfaces. 2017;155:323-331
Bajić Vladan P., Spremo-Potparević Biljana, Živković Lada, Cabarkapa Andrea, Kotur-Stevuljevic Jelena, Isenović Esma R., Sredojević Dušan, Vukoje Ivana D., Lazić Vesna M., Ahrenkiel Scott Phillip, Nedeljković Jovan, "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro" Colloids and Surfaces. B: Biointerfaces, 155 (2017):323-331,
https://doi.org/10.1016/j.colsurfb.2017.04.032 .
16
15
17

Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells

Živković, Lada; Borozan, Sunčica Z.; Cabarkapa, Andrea; Topalović, Dijana; Ciptasari, Ummi; Bajić, Vladan P.; Spremo-Potparević, Biljana

(2017)

TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica Z.
AU  - Cabarkapa, Andrea
AU  - Topalović, Dijana
AU  - Ciptasari, Ummi
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1458
AB  - The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells
DO  - 10.1155/2017/8759764
ER  - 
@article{
author = "Živković, Lada and Borozan, Sunčica Z. and Cabarkapa, Andrea and Topalović, Dijana and Ciptasari, Ummi and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1458",
abstract = "The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells",
doi = "10.1155/2017/8759764"
}
Živković, L., Borozan, S. Z., Cabarkapa, A., Topalović, D., Ciptasari, U., Bajić, V. P.,& Spremo-Potparević, B. (2017). Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells.
Oxidative Medicine and Cellular Longevity.
https://doi.org/10.1155/2017/8759764
Živković L, Borozan SZ, Cabarkapa A, Topalović D, Ciptasari U, Bajić VP, Spremo-Potparević B. Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells. Oxidative Medicine and Cellular Longevity. 2017;
Živković Lada, Borozan Sunčica Z., Cabarkapa Andrea, Topalović Dijana, Ciptasari Ummi, Bajić Vladan P., Spremo-Potparević Biljana, "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells" Oxidative Medicine and Cellular Longevity (2017),
https://doi.org/10.1155/2017/8759764 .
12
11
15

Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy

Cabarkapa, Andrea; Dekanski, Dragana; Živković, Lada; Milanovic-Cabarkapa, Mirjana; Bajić, Vladan P.; Topalović, Dijana; Giampieri, Francesca; Gasparrini, Massimiliano; Battino, Maurizio; Spremo-Potparević, Biljana

(2017)

TY  - JOUR
AU  - Cabarkapa, Andrea
AU  - Dekanski, Dragana
AU  - Živković, Lada
AU  - Milanovic-Cabarkapa, Mirjana
AU  - Bajić, Vladan P.
AU  - Topalović, Dijana
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Battino, Maurizio
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1679
AB  - The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations GT = 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy. (C) 2016 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy
VL  - 106
SP  - 616
EP  - 623
DO  - 10.1016/j.fct.2016.12.023
ER  - 
@article{
author = "Cabarkapa, Andrea and Dekanski, Dragana and Živković, Lada and Milanovic-Cabarkapa, Mirjana and Bajić, Vladan P. and Topalović, Dijana and Giampieri, Francesca and Gasparrini, Massimiliano and Battino, Maurizio and Spremo-Potparević, Biljana",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1679",
abstract = "The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations GT = 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy. (C) 2016 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy",
volume = "106",
pages = "616-623",
doi = "10.1016/j.fct.2016.12.023"
}
Cabarkapa, A., Dekanski, D., Živković, L., Milanovic-Cabarkapa, M., Bajić, V. P., Topalović, D., Giampieri, F., Gasparrini, M., Battino, M.,& Spremo-Potparević, B. (2017). Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy.
Food and Chemical Toxicology, 106, 616-623.
https://doi.org/10.1016/j.fct.2016.12.023
Cabarkapa A, Dekanski D, Živković L, Milanovic-Cabarkapa M, Bajić VP, Topalović D, Giampieri F, Gasparrini M, Battino M, Spremo-Potparević B. Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy. Food and Chemical Toxicology. 2017;106:616-623
Cabarkapa Andrea, Dekanski Dragana, Živković Lada, Milanovic-Cabarkapa Mirjana, Bajić Vladan P., Topalović Dijana, Giampieri Francesca, Gasparrini Massimiliano, Battino Maurizio, Spremo-Potparević Biljana, "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy" Food and Chemical Toxicology, 106 (2017):616-623,
https://doi.org/10.1016/j.fct.2016.12.023 .
11
7
7

Treatment of Alzheimers Disease: Classical Therapeutic Approach

Bajić, Vladan P.; Sudar, Emina; Spremo-Potparević, Biljana; Živković, Lada; Milićević, Zorka T.; Stanimirović, Julijana; Bogdanović, Nikola; Isenović, Esma R.

(2016)

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Sudar, Emina
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Milićević, Zorka T.
AU  - Stanimirović, Julijana
AU  - Bogdanović, Nikola
AU  - Isenović, Esma R.
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1039
AB  - Alzheimers disease (AD) is a complex and progressive neurodegenerative disorder, and represents the most common form of dementia. The number of people affected by AD is estimated to be doubled by the year of 2050, and more than 100 million people worldwide will be affected by this disease. Still, there is no reliable diagnostic test which would indicate pre-symptomatic conditions or an increased risk of developing AD. The only drugs approved by the FDA belong to the cholinesterase inhibitors (ChEI) group, such as donepezil, rivastigmine, galantamine and memantine that belongs to a class of drugs named receptor NMDA antagonists. Most mainstream pharmacotherapeutic approaches act by slowing the progression of the condition rather than to treat or prevent the cause of AD. In this review we are presenting literature data from recent research related to new avenues in the classical approach to prevention and treatment of AD.
T2  - Current Pharmaceutical Analysis
T1  - Treatment of Alzheimers Disease: Classical Therapeutic Approach
VL  - 12
IS  - 2
SP  - 82
EP  - 90
DO  - 10.2174/1573412911666150611184740
ER  - 
@article{
author = "Bajić, Vladan P. and Sudar, Emina and Spremo-Potparević, Biljana and Živković, Lada and Milićević, Zorka T. and Stanimirović, Julijana and Bogdanović, Nikola and Isenović, Esma R.",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1039",
abstract = "Alzheimers disease (AD) is a complex and progressive neurodegenerative disorder, and represents the most common form of dementia. The number of people affected by AD is estimated to be doubled by the year of 2050, and more than 100 million people worldwide will be affected by this disease. Still, there is no reliable diagnostic test which would indicate pre-symptomatic conditions or an increased risk of developing AD. The only drugs approved by the FDA belong to the cholinesterase inhibitors (ChEI) group, such as donepezil, rivastigmine, galantamine and memantine that belongs to a class of drugs named receptor NMDA antagonists. Most mainstream pharmacotherapeutic approaches act by slowing the progression of the condition rather than to treat or prevent the cause of AD. In this review we are presenting literature data from recent research related to new avenues in the classical approach to prevention and treatment of AD.",
journal = "Current Pharmaceutical Analysis",
title = "Treatment of Alzheimers Disease: Classical Therapeutic Approach",
volume = "12",
number = "2",
pages = "82-90",
doi = "10.2174/1573412911666150611184740"
}
Bajić, V. P., Sudar, E., Spremo-Potparević, B., Živković, L., Milićević, Z. T., Stanimirović, J., Bogdanović, N.,& Isenović, E. R. (2016). Treatment of Alzheimers Disease: Classical Therapeutic Approach.
Current Pharmaceutical Analysis, 12(2), 82-90.
https://doi.org/10.2174/1573412911666150611184740
Bajić VP, Sudar E, Spremo-Potparević B, Živković L, Milićević ZT, Stanimirović J, Bogdanović N, Isenović ER. Treatment of Alzheimers Disease: Classical Therapeutic Approach. Current Pharmaceutical Analysis. 2016;12(2):82-90
Bajić Vladan P., Sudar Emina, Spremo-Potparević Biljana, Živković Lada, Milićević Zorka T., Stanimirović Julijana, Bogdanović Nikola, Isenović Esma R., "Treatment of Alzheimers Disease: Classical Therapeutic Approach" Current Pharmaceutical Analysis, 12, no. 2 (2016):82-90,
https://doi.org/10.2174/1573412911666150611184740 .
15
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12

Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)

Živković, Lada; Cabarkapa, Andrea; Marcetic, Mirjana; Kovacevic, Nada; Bajić, Vladan P.; Jovicic, Snezana; Spremo-Potparević, Biljana

(2016)

TY  - JOUR
AU  - Živković, Lada
AU  - Cabarkapa, Andrea
AU  - Marcetic, Mirjana
AU  - Kovacevic, Nada
AU  - Bajić, Vladan P.
AU  - Jovicic, Snezana
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1121
AB  - The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.
T2  - Archives of biological sciences
T1  - Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)
VL  - 68
IS  - 1
SP  - 135
EP  - 144
DO  - 10.2298/ABS150512135Z
ER  - 
@article{
author = "Živković, Lada and Cabarkapa, Andrea and Marcetic, Mirjana and Kovacevic, Nada and Bajić, Vladan P. and Jovicic, Snezana and Spremo-Potparević, Biljana",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1121",
abstract = "The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.",
journal = "Archives of biological sciences",
title = "Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)",
volume = "68",
number = "1",
pages = "135-144",
doi = "10.2298/ABS150512135Z"
}
Živković, L., Cabarkapa, A., Marcetic, M., Kovacevic, N., Bajić, V. P., Jovicic, S.,& Spremo-Potparević, B. (2016). Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae).
Archives of biological sciences, 68(1), 135-144.
https://doi.org/10.2298/ABS150512135Z
Živković L, Cabarkapa A, Marcetic M, Kovacevic N, Bajić VP, Jovicic S, Spremo-Potparević B. Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae). Archives of biological sciences. 2016;68(1):135-144
Živković Lada, Cabarkapa Andrea, Marcetic Mirjana, Kovacevic Nada, Bajić Vladan P., Jovicic Snezana, Spremo-Potparević Biljana, "Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)" Archives of biological sciences, 68, no. 1 (2016):135-144,
https://doi.org/10.2298/ABS150512135Z .
2
1
3

Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study

Cabarkapa, Andrea; Živković, Lada; Borozan, Sunčica Z.; Zlatkovic-Svenda, Mirjana; Dekanski, Dragana; Jancic, Ivan; Radak-Perovic, Marija; Bajić, Vladan P.; Spremo-Potparević, Biljana

(2016)

TY  - JOUR
AU  - Cabarkapa, Andrea
AU  - Živković, Lada
AU  - Borozan, Sunčica Z.
AU  - Zlatkovic-Svenda, Mirjana
AU  - Dekanski, Dragana
AU  - Jancic, Ivan
AU  - Radak-Perovic, Marija
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1282
AB  - The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright (c) 2016 John Wiley and Sons, Ltd.
T2  - Phytotherapy Research
T1  - Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study
VL  - 30
IS  - 10
SP  - 1615
EP  - 1623
DO  - 10.1002/ptr.5662
ER  - 
@article{
author = "Cabarkapa, Andrea and Živković, Lada and Borozan, Sunčica Z. and Zlatkovic-Svenda, Mirjana and Dekanski, Dragana and Jancic, Ivan and Radak-Perovic, Marija and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1282",
abstract = "The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright (c) 2016 John Wiley and Sons, Ltd.",
journal = "Phytotherapy Research",
title = "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study",
volume = "30",
number = "10",
pages = "1615-1623",
doi = "10.1002/ptr.5662"
}
Cabarkapa, A., Živković, L., Borozan, S. Z., Zlatkovic-Svenda, M., Dekanski, D., Jancic, I., Radak-Perovic, M., Bajić, V. P.,& Spremo-Potparević, B. (2016). Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study.
Phytotherapy Research, 30(10), 1615-1623.
https://doi.org/10.1002/ptr.5662
Cabarkapa A, Živković L, Borozan SZ, Zlatkovic-Svenda M, Dekanski D, Jancic I, Radak-Perovic M, Bajić VP, Spremo-Potparević B. Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study. Phytotherapy Research. 2016;30(10):1615-1623
Cabarkapa Andrea, Živković Lada, Borozan Sunčica Z., Zlatkovic-Svenda Mirjana, Dekanski Dragana, Jancic Ivan, Radak-Perovic Marija, Bajić Vladan P., Spremo-Potparević Biljana, "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study" Phytotherapy Research, 30, no. 10 (2016):1615-1623,
https://doi.org/10.1002/ptr.5662 .
1
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6

Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay

Vasiljevic, Jovana; Živković, Lada; Cabarkapa, Andrea; Bajić, Vladan P.; Djelic, Ninoslav; Spremo-Potparević, Biljana

(2016)

TY  - JOUR
AU  - Vasiljevic, Jovana
AU  - Živković, Lada
AU  - Cabarkapa, Andrea
AU  - Bajić, Vladan P.
AU  - Djelic, Ninoslav
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1311
AB  - Context Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design The research team designed a pilot study. Setting The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention Four concentrations of the CS extract125 mu g/mL, 250 mu g/mL, 500 mu g/mL, and 1000 mu g/mL-were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A-undamaged cells with no tail ( LT 5% damaged DNA); (2) class B-low-level damage (5%-20%); (3) class C-medium-level damage (20%-40%); (4) class D-high-level damage (40%-95%), and (5) class E-total destruction ( GT 95%). Results The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-mu g/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.
T2  - Alternative Therapies in Health and Medicine
T1  - Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay
VL  - 22
SP  - 24
EP  - 31
ER  - 
@article{
author = "Vasiljevic, Jovana and Živković, Lada and Cabarkapa, Andrea and Bajić, Vladan P. and Djelic, Ninoslav and Spremo-Potparević, Biljana",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1311",
abstract = "Context Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design The research team designed a pilot study. Setting The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention Four concentrations of the CS extract125 mu g/mL, 250 mu g/mL, 500 mu g/mL, and 1000 mu g/mL-were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A-undamaged cells with no tail ( LT 5% damaged DNA); (2) class B-low-level damage (5%-20%); (3) class C-medium-level damage (20%-40%); (4) class D-high-level damage (40%-95%), and (5) class E-total destruction ( GT 95%). Results The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-mu g/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.",
journal = "Alternative Therapies in Health and Medicine",
title = "Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay",
volume = "22",
pages = "24-31"
}
Vasiljevic, J., Živković, L., Cabarkapa, A., Bajić, V. P., Djelic, N.,& Spremo-Potparević, B. (2016). Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay.
Alternative Therapies in Health and Medicine, 22, 24-31.
Vasiljevic J, Živković L, Cabarkapa A, Bajić VP, Djelic N, Spremo-Potparević B. Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay. Alternative Therapies in Health and Medicine. 2016;22:24-31
Vasiljevic Jovana, Živković Lada, Cabarkapa Andrea, Bajić Vladan P., Djelic Ninoslav, Spremo-Potparević Biljana, "Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay" Alternative Therapies in Health and Medicine, 22 (2016):24-31
5

Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps

Cilerdzic, Jasmina; Stajic, Mirjana; Živković, Lada; Vukojevic, Jelena; Bajić, Vladan P.; Spremo-Potparević, Biljana

(2016)

TY  - JOUR
AU  - Cilerdzic, Jasmina
AU  - Stajic, Mirjana
AU  - Živković, Lada
AU  - Vukojevic, Jelena
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1448
AB  - Ganoderma lucidum is traditionally used in Eastern medicine to preserve vitality, promote longevity, and treat disease. It possesses immunomodulatory, antitumor, antimicrobial, and antiaging activities, among others, but one of the most important is its antioxidant property, which is the basis for other effects, because free radicals trigger many diseases. The substrate commonly used for commercial cultivation of G. lucidum is not environmenwas to analyze the effect of substrate composition on the bioactivity of G. lucidum basidiocarps. G. lucidum was cultivated on 2 different substrates: (1) a mixture of wheat straw, grapevine branches, and wheat bran, and (2) wheat straw. Commercial fruiting bodies, cultivated on oak sawdust, were used as the control. 1,1- diphenyl- 2- picrylhydrazyl (DPPH) The comet test was performed to detect the degree of DNA damage in the cells that were exposed to G. lucidum extracts before and after the effect of oxidants. Higher antioxidative potential was observed for the extract of G. lucidum basidiocarps cultivated on wheat straw compared with that from the mixed substrate and especially with commercial ones. The alternatively cultivated basidiocarps also showed stronger antigenotoxic potential compared with commercial ones. The study showed that fruiting bodies produced on wheat straw, one of the most accessible and cheapest crop residues, are more potent antioxidant and antigenotoxic agents than commercially cultivated ones.
T2  - International Journal of Medicinal Mushrooms
T1  - Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps
VL  - 18
IS  - 12
SP  - 1061
EP  - 1069
DO  - 10.1615/IntJMedMushrooms.v18.i12.10
ER  - 
@article{
author = "Cilerdzic, Jasmina and Stajic, Mirjana and Živković, Lada and Vukojevic, Jelena and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1448",
abstract = "Ganoderma lucidum is traditionally used in Eastern medicine to preserve vitality, promote longevity, and treat disease. It possesses immunomodulatory, antitumor, antimicrobial, and antiaging activities, among others, but one of the most important is its antioxidant property, which is the basis for other effects, because free radicals trigger many diseases. The substrate commonly used for commercial cultivation of G. lucidum is not environmenwas to analyze the effect of substrate composition on the bioactivity of G. lucidum basidiocarps. G. lucidum was cultivated on 2 different substrates: (1) a mixture of wheat straw, grapevine branches, and wheat bran, and (2) wheat straw. Commercial fruiting bodies, cultivated on oak sawdust, were used as the control. 1,1- diphenyl- 2- picrylhydrazyl (DPPH) The comet test was performed to detect the degree of DNA damage in the cells that were exposed to G. lucidum extracts before and after the effect of oxidants. Higher antioxidative potential was observed for the extract of G. lucidum basidiocarps cultivated on wheat straw compared with that from the mixed substrate and especially with commercial ones. The alternatively cultivated basidiocarps also showed stronger antigenotoxic potential compared with commercial ones. The study showed that fruiting bodies produced on wheat straw, one of the most accessible and cheapest crop residues, are more potent antioxidant and antigenotoxic agents than commercially cultivated ones.",
journal = "International Journal of Medicinal Mushrooms",
title = "Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps",
volume = "18",
number = "12",
pages = "1061-1069",
doi = "10.1615/IntJMedMushrooms.v18.i12.10"
}
Cilerdzic, J., Stajic, M., Živković, L., Vukojevic, J., Bajić, V. P.,& Spremo-Potparević, B. (2016). Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps.
International Journal of Medicinal Mushrooms, 18(12), 1061-1069.
https://doi.org/10.1615/IntJMedMushrooms.v18.i12.10
Cilerdzic J, Stajic M, Živković L, Vukojevic J, Bajić VP, Spremo-Potparević B. Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps. International Journal of Medicinal Mushrooms. 2016;18(12):1061-1069
Cilerdzic Jasmina, Stajic Mirjana, Živković Lada, Vukojevic Jelena, Bajić Vladan P., Spremo-Potparević Biljana, "Genoprotective Capacity of Alternatively Cultivated Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Basidiocarps" International Journal of Medicinal Mushrooms, 18, no. 12 (2016):1061-1069,
https://doi.org/10.1615/IntJMedMushrooms.v18.i12.10 .
1
3
3
4

Implications of oxidative stress in occupational exposure to lead on a cellular level

Cabarkapa, Andrea; Borozan, Sunčica Z.; Živković, Lada; Milanovic-Cabarkapa, Mirjana; Stojanovic, Srdan; Bajić, Vladan P.; Spremo-Potparević, Biljana

(2015)

TY  - JOUR
AU  - Cabarkapa, Andrea
AU  - Borozan, Sunčica Z.
AU  - Živković, Lada
AU  - Milanovic-Cabarkapa, Mirjana
AU  - Stojanovic, Srdan
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/703
AB  - The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.
T2  - Toxicological and Environmental Chemistry
T1  - Implications of oxidative stress in occupational exposure to lead on a cellular level
VL  - 97
IS  - 6
SP  - 799
EP  - 813
DO  - 10.1080/02772248.2015.1060973
ER  - 
@article{
author = "Cabarkapa, Andrea and Borozan, Sunčica Z. and Živković, Lada and Milanovic-Cabarkapa, Mirjana and Stojanovic, Srdan and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/703",
abstract = "The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.",
journal = "Toxicological and Environmental Chemistry",
title = "Implications of oxidative stress in occupational exposure to lead on a cellular level",
volume = "97",
number = "6",
pages = "799-813",
doi = "10.1080/02772248.2015.1060973"
}
Cabarkapa, A., Borozan, S. Z., Živković, L., Milanovic-Cabarkapa, M., Stojanovic, S., Bajić, V. P.,& Spremo-Potparević, B. (2015). Implications of oxidative stress in occupational exposure to lead on a cellular level.
Toxicological and Environmental Chemistry, 97(6), 799-813.
https://doi.org/10.1080/02772248.2015.1060973
Cabarkapa A, Borozan SZ, Živković L, Milanovic-Cabarkapa M, Stojanovic S, Bajić VP, Spremo-Potparević B. Implications of oxidative stress in occupational exposure to lead on a cellular level. Toxicological and Environmental Chemistry. 2015;97(6):799-813
Cabarkapa Andrea, Borozan Sunčica Z., Živković Lada, Milanovic-Cabarkapa Mirjana, Stojanovic Srdan, Bajić Vladan P., Spremo-Potparević Biljana, "Implications of oxidative stress in occupational exposure to lead on a cellular level" Toxicological and Environmental Chemistry, 97, no. 6 (2015):799-813,
https://doi.org/10.1080/02772248.2015.1060973 .
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Cohesion and the aneuploid phenotype in Alzheimers disease: A tale of genome instability

Bajić, Vladan P.; Spremo-Potparević, Biljana; Živković, Lada; Isenović, Esma R.; Arendt, Thomas

(2015)

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Isenović, Esma R.
AU  - Arendt, Thomas
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/664
AB  - Neurons are postmitotic cells that are in permanent cell cycle arrest. However, components of the cell cycle machinery that are expressed in Alzheimers disease (AD) neurons are showing features of a cycling cell and those attributed to a postmitotic cell as well. Furthermore, the unique physiological operations taking place in neurons, ascribed to core cell cycle regulators are also key regulators in cell division. Functions of these cell cycle regulators include neuronal migration, axonal elongation, axon pruning, dendrite morphogenesis and synaptic maturation and plasticity. In this review, we focus on cohesion and cohesion related proteins in reference to their neuronal functions and how impaired centromere/cohesion dynamics may connect cell cycle dysfunction to aneuploidy in AD. (C) 2015 Elsevier Ltd. All rights reserved.
T2  - Neuroscience and Biobehavioral Reviews
T1  - Cohesion and the aneuploid phenotype in Alzheimers disease: A tale of genome instability
VL  - 55
SP  - 365
EP  - 374
DO  - 10.1016/j.neubiorev.2015.05.010
ER  - 
@article{
author = "Bajić, Vladan P. and Spremo-Potparević, Biljana and Živković, Lada and Isenović, Esma R. and Arendt, Thomas",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/664",
abstract = "Neurons are postmitotic cells that are in permanent cell cycle arrest. However, components of the cell cycle machinery that are expressed in Alzheimers disease (AD) neurons are showing features of a cycling cell and those attributed to a postmitotic cell as well. Furthermore, the unique physiological operations taking place in neurons, ascribed to core cell cycle regulators are also key regulators in cell division. Functions of these cell cycle regulators include neuronal migration, axonal elongation, axon pruning, dendrite morphogenesis and synaptic maturation and plasticity. In this review, we focus on cohesion and cohesion related proteins in reference to their neuronal functions and how impaired centromere/cohesion dynamics may connect cell cycle dysfunction to aneuploidy in AD. (C) 2015 Elsevier Ltd. All rights reserved.",
journal = "Neuroscience and Biobehavioral Reviews",
title = "Cohesion and the aneuploid phenotype in Alzheimers disease: A tale of genome instability",
volume = "55",
pages = "365-374",
doi = "10.1016/j.neubiorev.2015.05.010"
}
Bajić, V. P., Spremo-Potparević, B., Živković, L., Isenović, E. R.,& Arendt, T. (2015). Cohesion and the aneuploid phenotype in Alzheimers disease: A tale of genome instability.
Neuroscience and Biobehavioral Reviews, 55, 365-374.
https://doi.org/10.1016/j.neubiorev.2015.05.010
Bajić VP, Spremo-Potparević B, Živković L, Isenović ER, Arendt T. Cohesion and the aneuploid phenotype in Alzheimers disease: A tale of genome instability. Neuroscience and Biobehavioral Reviews. 2015;55:365-374
Bajić Vladan P., Spremo-Potparević Biljana, Živković Lada, Isenović Esma R., Arendt Thomas, "Cohesion and the aneuploid phenotype in Alzheimers disease: A tale of genome instability" Neuroscience and Biobehavioral Reviews, 55 (2015):365-374,
https://doi.org/10.1016/j.neubiorev.2015.05.010 .
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21

Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro

Topalović Žukovec, Dijana; Živković, Lada; Cabarkapa, Andrea; Djelic, Ninoslav; Bajić, Vladan P.; Dekanski, Dragana; Spremo-Potparević, Biljana

(2015)

TY  - JOUR
AU  - Topalović Žukovec, Dijana
AU  - Živković, Lada
AU  - Cabarkapa, Andrea
AU  - Djelic, Ninoslav
AU  - Bajić, Vladan P.
AU  - Dekanski, Dragana
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/432
AB  - The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks. Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants. The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay. Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment. Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity. The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P LT 0.05). Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect. This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro
DO  - 10.1155/2015/762192
ER  - 
@article{
author = "Topalović Žukovec, Dijana and Živković, Lada and Cabarkapa, Andrea and Djelic, Ninoslav and Bajić, Vladan P. and Dekanski, Dragana and Spremo-Potparević, Biljana",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/432",
abstract = "The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks. Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants. The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay. Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment. Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity. The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P LT 0.05). Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect. This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro",
doi = "10.1155/2015/762192"
}
Topalović Žukovec, D., Živković, L., Cabarkapa, A., Djelic, N., Bajić, V. P., Dekanski, D.,& Spremo-Potparević, B. (2015). Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro.
Oxidative Medicine and Cellular Longevity.
https://doi.org/10.1155/2015/762192
Topalović Žukovec D, Živković L, Cabarkapa A, Djelic N, Bajić VP, Dekanski D, Spremo-Potparević B. Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. Oxidative Medicine and Cellular Longevity. 2015;
Topalović Žukovec Dijana, Živković Lada, Cabarkapa Andrea, Djelic Ninoslav, Bajić Vladan P., Dekanski Dragana, Spremo-Potparević Biljana, "Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro" Oxidative Medicine and Cellular Longevity (2015),
https://doi.org/10.1155/2015/762192 .
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Non-Classical Therapeutic Approach in the Treatment of Alzheimers Disease: A Mini Review

Bajić, Vladan P.; Spremo-Potparević, Biljana; Živković, Lada; Sudar, Emina; Zafirović, Sonja; Obradović, Milan M.; Isenović, Esma R.

(2015)

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Sudar, Emina
AU  - Zafirović, Sonja
AU  - Obradović, Milan M.
AU  - Isenović, Esma R.
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/398
AB  - Alzheimers disease (AD) is a multi factorial disease, related to the loss of neurons and synapses in cerebral cortex and subcortical structures, leading to degenerative changes and atrophy. Despite abundance of facts related to AD and its pathology, the only drugs used in the prevention and treatment are those from the cholinesterase inhibitors group. However, there is growing evidence that a non-classical therapeutic approach in the treatment of AD has beneficial effects. In this review we summarized recent literature data related to the non-classical drugs for the treatment of AD predominantly used in clinical testing, such as amyloid aggregation inhibitors, beta-sheet breakers, antioxidants, estrogens and immunotherapeutics.
T2  - Letters in Drug Design and Discovery
T1  - Non-Classical Therapeutic Approach in the Treatment of Alzheimers Disease: A Mini Review
VL  - 12
IS  - 2
SP  - 158
EP  - 164
ER  - 
@article{
author = "Bajić, Vladan P. and Spremo-Potparević, Biljana and Živković, Lada and Sudar, Emina and Zafirović, Sonja and Obradović, Milan M. and Isenović, Esma R.",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/398",
abstract = "Alzheimers disease (AD) is a multi factorial disease, related to the loss of neurons and synapses in cerebral cortex and subcortical structures, leading to degenerative changes and atrophy. Despite abundance of facts related to AD and its pathology, the only drugs used in the prevention and treatment are those from the cholinesterase inhibitors group. However, there is growing evidence that a non-classical therapeutic approach in the treatment of AD has beneficial effects. In this review we summarized recent literature data related to the non-classical drugs for the treatment of AD predominantly used in clinical testing, such as amyloid aggregation inhibitors, beta-sheet breakers, antioxidants, estrogens and immunotherapeutics.",
journal = "Letters in Drug Design and Discovery",
title = "Non-Classical Therapeutic Approach in the Treatment of Alzheimers Disease: A Mini Review",
volume = "12",
number = "2",
pages = "158-164"
}
Bajić, V. P., Spremo-Potparević, B., Živković, L., Sudar, E., Zafirović, S., Obradović, M. M.,& Isenović, E. R. (2015). Non-Classical Therapeutic Approach in the Treatment of Alzheimers Disease: A Mini Review.
Letters in Drug Design and Discovery, 12(2), 158-164.
Bajić VP, Spremo-Potparević B, Živković L, Sudar E, Zafirović S, Obradović MM, Isenović ER. Non-Classical Therapeutic Approach in the Treatment of Alzheimers Disease: A Mini Review. Letters in Drug Design and Discovery. 2015;12(2):158-164
Bajić Vladan P., Spremo-Potparević Biljana, Živković Lada, Sudar Emina, Zafirović Sonja, Obradović Milan M., Isenović Esma R., "Non-Classical Therapeutic Approach in the Treatment of Alzheimers Disease: A Mini Review" Letters in Drug Design and Discovery, 12, no. 2 (2015):158-164
3