TY  - CONF
AU  - Milošević, Maja
AU  - Petrović, S.
AU  - Horvat, Anica
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9497
AB  - Effects of increasing concentrations of metal salts SrCl2, CsCl, CrCl3, CdCl2, and HgCl2 on rat ovarian plasma membrane ecto-ATPase activity were investigated. CrCl3, CdCl2, and HgCl2 exert total inhibition of enzyme activity in the presence of 0.01 M and 0.1 M respectively. SrCl2 and CsCl exhibit up to 25% of inhibition. According to the IC50, ecto-ATPase possesses greater sensibility to Cd2+ (IC50 is 0.887 mM) > Cr3+ (IC50 is 1.936 mM) > Hg2+ (IC50 is 4.39 mM). All investigated ions exert negative cooperativity (n<1). Physico-chemical properties of the metal are of importance in metal toxicity. Cr3+ and Cd2+ ions, with lower radius may inhibit ecto-ATPase activity by binding to hydrolytic site or by replacing Mg2+ in Mg-ATP, a substrate of the enzyme. Hg2+, as a larger ion probably inhibits the enzyme activity through conformational changing the enzyme by binding to S-S or -SH groups on the site distinct to hydrolytic one. By inhibiting the enzyme activity these metals may affect maturation and release of oocytes as well as synthesis and release of gonadal hormones and decrease the fertility of mammals
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - Effects of metal ions on ecto-ATPase activity in plasma membrane isolated from the rat ovary
VL  - 1
SP  - 368
EP  - 370
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9497
ER  - 

@conference{
author = "Milošević, Maja and Petrović, S. and Horvat, Anica",
year = "2004",
abstract = "Effects of increasing concentrations of metal salts SrCl2, CsCl, CrCl3, CdCl2, and HgCl2 on rat ovarian plasma membrane ecto-ATPase activity were investigated. CrCl3, CdCl2, and HgCl2 exert total inhibition of enzyme activity in the presence of 0.01 M and 0.1 M respectively. SrCl2 and CsCl exhibit up to 25% of inhibition. According to the IC50, ecto-ATPase possesses greater sensibility to Cd2+ (IC50 is 0.887 mM) > Cr3+ (IC50 is 1.936 mM) > Hg2+ (IC50 is 4.39 mM). All investigated ions exert negative cooperativity (n<1). Physico-chemical properties of the metal are of importance in metal toxicity. Cr3+ and Cd2+ ions, with lower radius may inhibit ecto-ATPase activity by binding to hydrolytic site or by replacing Mg2+ in Mg-ATP, a substrate of the enzyme. Hg2+, as a larger ion probably inhibits the enzyme activity through conformational changing the enzyme by binding to S-S or -SH groups on the site distinct to hydrolytic one. By inhibiting the enzyme activity these metals may affect maturation and release of oocytes as well as synthesis and release of gonadal hormones and decrease the fertility of mammals",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Effects of metal ions on ecto-ATPase activity in plasma membrane isolated from the rat ovary",
volume = "1",
pages = "368-370",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9497"
}

Milošević, M., Petrović, S.,& Horvat, A.. (2004). Effects of metal ions on ecto-ATPase activity in plasma membrane isolated from the rat ovary. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 368-370.
https://hdl.handle.net/21.15107/rcub_vinar_9497

Milošević M, Petrović S, Horvat A. Effects of metal ions on ecto-ATPase activity in plasma membrane isolated from the rat ovary. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:368-370.
https://hdl.handle.net/21.15107/rcub_vinar_9497 .

Milošević, Maja, Petrović, S., Horvat, Anica, "Effects of metal ions on ecto-ATPase activity in plasma membrane isolated from the rat ovary" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):368-370,
https://hdl.handle.net/21.15107/rcub_vinar_9497 .