TY  - JOUR
AU  - Giordano, Ady
AU  - Morales-Tapia, Pablo
AU  - Moncada-Basualto, Mauricio
AU  - Pozo-Martínez, Josué
AU  - Olea-Azar, Claudio
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10130
AB  - Plant biochemistry studies have increased in recent years due to their potential to improve human health. Argylia radiata is an extremophile plant with an interesting polyphenolic profile. However, its biomass is scarce and occasionally available. Argylia in vitro biomass was obtained from tissue culture and compared with in vivo roots regarding its polyphenolic and flavonoid content. Different solvents were used to prepare extracts from the in vitro tissue of callus and aerial plant organs and in vivo roots. UPLC-MS/MS was used to assess the chemical composition of each extract. ORAC-FL and scavenging of free radicals (DPPH and OH) methods were used to determine the antioxidant capacity of extracts. Furthermore, the biological activity of the extracts was established using the cellular antioxidant activity method. The vitroplants were a good source of polyphenols (25–68 mg GAE/100 g tissue FW), and methanol was the most efficient solvent. Eight polyphenolic compounds were identified, and their antioxidant properties were investigated by different chemical methods with EPR demonstrating its specific scavenging activity against free radicals. All extracts showed cellular dose-dependent antioxidant activity. The methanolic extract of vitroplants showed the highest cellular antioxidant activity (44.6% and 51%) at 1 and 10 µg/mL of extract, respectively. Vitroplants of A. radiata are proposed as a biotechnological product as a source of antioxidant compounds with multiple applications.
T2  - Molecules
T1  - Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots
VL  - 27
IS  - 3
SP  - 610
DO  - 10.3390/molecules27030610
ER  - 

@article{
author = "Giordano, Ady and Morales-Tapia, Pablo and Moncada-Basualto, Mauricio and Pozo-Martínez, Josué and Olea-Azar, Claudio and Nešić, Aleksandra and Cabrera-Barjas, Gustavo",
year = "2022",
abstract = "Plant biochemistry studies have increased in recent years due to their potential to improve human health. Argylia radiata is an extremophile plant with an interesting polyphenolic profile. However, its biomass is scarce and occasionally available. Argylia in vitro biomass was obtained from tissue culture and compared with in vivo roots regarding its polyphenolic and flavonoid content. Different solvents were used to prepare extracts from the in vitro tissue of callus and aerial plant organs and in vivo roots. UPLC-MS/MS was used to assess the chemical composition of each extract. ORAC-FL and scavenging of free radicals (DPPH and OH) methods were used to determine the antioxidant capacity of extracts. Furthermore, the biological activity of the extracts was established using the cellular antioxidant activity method. The vitroplants were a good source of polyphenols (25–68 mg GAE/100 g tissue FW), and methanol was the most efficient solvent. Eight polyphenolic compounds were identified, and their antioxidant properties were investigated by different chemical methods with EPR demonstrating its specific scavenging activity against free radicals. All extracts showed cellular dose-dependent antioxidant activity. The methanolic extract of vitroplants showed the highest cellular antioxidant activity (44.6% and 51%) at 1 and 10 µg/mL of extract, respectively. Vitroplants of A. radiata are proposed as a biotechnological product as a source of antioxidant compounds with multiple applications.",
journal = "Molecules",
title = "Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots",
volume = "27",
number = "3",
pages = "610",
doi = "10.3390/molecules27030610"
}

Giordano, A., Morales-Tapia, P., Moncada-Basualto, M., Pozo-Martínez, J., Olea-Azar, C., Nešić, A.,& Cabrera-Barjas, G.. (2022). Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots. in Molecules, 27(3), 610.
https://doi.org/10.3390/molecules27030610

Giordano A, Morales-Tapia P, Moncada-Basualto M, Pozo-Martínez J, Olea-Azar C, Nešić A, Cabrera-Barjas G. Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots. in Molecules. 2022;27(3):610.
doi:10.3390/molecules27030610 .

Giordano, Ady, Morales-Tapia, Pablo, Moncada-Basualto, Mauricio, Pozo-Martínez, Josué, Olea-Azar, Claudio, Nešić, Aleksandra, Cabrera-Barjas, Gustavo, "Polyphenolic Composition and Antioxidant Activity (ORAC, EPR and Cellular) of Different Extracts of Argylia radiata Vitroplants and Natural Roots" in Molecules, 27, no. 3 (2022):610,
https://doi.org/10.3390/molecules27030610 . .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Meseldžija, Slađana
AU  - Cabrera-Barjas, Gustavo
AU  - Onjia, Antonije E.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10149
AB  - Pectin is a natural biopolymer with broad applications in the food industry and it is suitable to prepare edible films to prolong food shelf-life. However, the main limitation of pectin-based films is their poor mechanical and barrier properties. Zeolite Y is a hydrophobic clay that can be used as film reinforcement material to improve its physicochemical and mechanical properties. In this work, the influence of high methoxyl citrus and apple pectin on physicochemical properties of biopolymer films modified with zeolite Y (0.05–0.2 wt%) was investigated. The films were characterized by FTIR, TGA, WAXD, mechanical analysis, and water vapor permeability analysis, and a potential film application is presented. The WAXD and FTIR analysis demonstrated that the strongest interaction between pectin chains and zeolite Y occurred when citrus high methylated pectin was used. Adding 0.2 wt% of zeolite Y into citrus high methylated pectin matrix enhanced the tensile strength by 66%, thermal stability by 13%, and water vapor barrier by 54%. In addition, fruit shelf-life test was performed, where strawberries were sealed in film. It was shown that sealed strawberries maintained a better color and healthy appearance than the control treatment after 7 days at 10◦ C. This study enabled the development of biocomposite films with improved properties for potential application in food packaging. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
T2  - Foods
T1  - Novel Biocomposite Films Based on High Methoxyl Pectin Reinforced with Zeolite Y for Food Packaging Applications
VL  - 11
IS  - 3
SP  - 360
DO  - 10.3390/foods11030360
ER  - 

@article{
author = "Nešić, Aleksandra and Meseldžija, Slađana and Cabrera-Barjas, Gustavo and Onjia, Antonije E.",
year = "2022",
abstract = "Pectin is a natural biopolymer with broad applications in the food industry and it is suitable to prepare edible films to prolong food shelf-life. However, the main limitation of pectin-based films is their poor mechanical and barrier properties. Zeolite Y is a hydrophobic clay that can be used as film reinforcement material to improve its physicochemical and mechanical properties. In this work, the influence of high methoxyl citrus and apple pectin on physicochemical properties of biopolymer films modified with zeolite Y (0.05–0.2 wt%) was investigated. The films were characterized by FTIR, TGA, WAXD, mechanical analysis, and water vapor permeability analysis, and a potential film application is presented. The WAXD and FTIR analysis demonstrated that the strongest interaction between pectin chains and zeolite Y occurred when citrus high methylated pectin was used. Adding 0.2 wt% of zeolite Y into citrus high methylated pectin matrix enhanced the tensile strength by 66%, thermal stability by 13%, and water vapor barrier by 54%. In addition, fruit shelf-life test was performed, where strawberries were sealed in film. It was shown that sealed strawberries maintained a better color and healthy appearance than the control treatment after 7 days at 10◦ C. This study enabled the development of biocomposite films with improved properties for potential application in food packaging. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
journal = "Foods",
title = "Novel Biocomposite Films Based on High Methoxyl Pectin Reinforced with Zeolite Y for Food Packaging Applications",
volume = "11",
number = "3",
pages = "360",
doi = "10.3390/foods11030360"
}

Nešić, A., Meseldžija, S., Cabrera-Barjas, G.,& Onjia, A. E.. (2022). Novel Biocomposite Films Based on High Methoxyl Pectin Reinforced with Zeolite Y for Food Packaging Applications. in Foods, 11(3), 360.
https://doi.org/10.3390/foods11030360

Nešić A, Meseldžija S, Cabrera-Barjas G, Onjia AE. Novel Biocomposite Films Based on High Methoxyl Pectin Reinforced with Zeolite Y for Food Packaging Applications. in Foods. 2022;11(3):360.
doi:10.3390/foods11030360 .

Nešić, Aleksandra, Meseldžija, Slađana, Cabrera-Barjas, Gustavo, Onjia, Antonije E., "Novel Biocomposite Films Based on High Methoxyl Pectin Reinforced with Zeolite Y for Food Packaging Applications" in Foods, 11, no. 3 (2022):360,
https://doi.org/10.3390/foods11030360 . .