NASA (NNX09AV07A)

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NASA (NNX09AV07A)

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DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells

Dojčilović, Radovan; Pajović, Jelena D.; Božanić, Dušan K.; Jović, Nataša G.; Pavlović, Vera P.; Pavlović, Vladimir B.; Lenhardt Acković, Lea; Zeković, Ivana Lj.; Dramićanin, Miroslav; Kaščakova, Slavka; Refregiers, Matthieu; Rašić, Goran; Vlahović, Branislav; Đoković, Vladimir

(2018)

TY  - JOUR
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Božanić, Dušan K.
AU  - Jović, Nataša G.
AU  - Pavlović, Vera P.
AU  - Pavlović, Vladimir B.
AU  - Lenhardt Acković, Lea
AU  - Zeković, Ivana Lj.
AU  - Dramićanin, Miroslav
AU  - Kaščakova, Slavka
AU  - Refregiers, Matthieu
AU  - Rašić, Goran
AU  - Vlahović, Branislav
AU  - Đoković, Vladimir
PY  - 2018
UR  - http://stacks.iop.org/2053-1583/5/i=4/a=045019?key=crossref.f455f07bdfb3469c8077963ff0f38f95
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7909
AB  - The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp2 and sp3 carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by ~2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery. © 2018 IOP Publishing Ltd.
T2  - 2D Materials
T1  - DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells
VL  - 5
IS  - 4
SP  - 045019
DO  - 10.1088/2053-1583/aad72b
ER  - 
@article{
author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Jović, Nataša G. and Pavlović, Vera P. and Pavlović, Vladimir B. and Lenhardt Acković, Lea and Zeković, Ivana Lj. and Dramićanin, Miroslav and Kaščakova, Slavka and Refregiers, Matthieu and Rašić, Goran and Vlahović, Branislav and Đoković, Vladimir",
year = "2018",
abstract = "The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp2 and sp3 carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by ~2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery. © 2018 IOP Publishing Ltd.",
journal = "2D Materials",
title = "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells",
volume = "5",
number = "4",
pages = "045019",
doi = "10.1088/2053-1583/aad72b"
}
Dojčilović, R., Pajović, J. D., Božanić, D. K., Jović, N. G., Pavlović, V. P., Pavlović, V. B., Lenhardt Acković, L., Zeković, I. Lj., Dramićanin, M., Kaščakova, S., Refregiers, M., Rašić, G., Vlahović, B.,& Đoković, V.. (2018). DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2D Materials, 5(4), 045019.
https://doi.org/10.1088/2053-1583/aad72b
Dojčilović R, Pajović JD, Božanić DK, Jović NG, Pavlović VP, Pavlović VB, Lenhardt Acković L, Zeković IL, Dramićanin M, Kaščakova S, Refregiers M, Rašić G, Vlahović B, Đoković V. DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2D Materials. 2018;5(4):045019.
doi:10.1088/2053-1583/aad72b .
Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Jović, Nataša G., Pavlović, Vera P., Pavlović, Vladimir B., Lenhardt Acković, Lea, Zeković, Ivana Lj., Dramićanin, Miroslav, Kaščakova, Slavka, Refregiers, Matthieu, Rašić, Goran, Vlahović, Branislav, Đoković, Vladimir, "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells" in 2D Materials, 5, no. 4 (2018):045019,
https://doi.org/10.1088/2053-1583/aad72b . .
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Structural and electrical properties of ferroelectric poly(vinylidene fluoride) and mechanically activated ZnO nanoparticle composite films

Peleš, Adriana; Aleksić, Obrad S.; Pavlović, Vera P.; Đoković, Vladimir; Dojčilović, Radovan; Nikolić, Zoran M.; Marinković, Filip S.; Mitrić, Miodrag; Blagojević, Vladimir D.; Vlahović, Branislav; Pavlović, Vladimir B.

(2018)

TY  - JOUR
AU  - Peleš, Adriana
AU  - Aleksić, Obrad S.
AU  - Pavlović, Vera P.
AU  - Đoković, Vladimir
AU  - Dojčilović, Radovan
AU  - Nikolić, Zoran M.
AU  - Marinković, Filip S.
AU  - Mitrić, Miodrag
AU  - Blagojević, Vladimir D.
AU  - Vlahović, Branislav
AU  - Pavlović, Vladimir B.
PY  - 2018
UR  - http://stacks.iop.org/1402-4896/93/i=10/a=105801?key=crossref.54536d8babe95dbc820e50211f592252
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7898
AB  - The influence of the mechanical activation of ZnO nanoparticle fillers on the structural and electrical properties of the matrix of poly(vinylidenefluoride)-ZnO (PVDF-ZnO) films was investigated. Transmission electron microscopy and scanning electron microscopy analyses showed that mechanical activation in a high energy planetary ball mill reduces the size of ZnO particles. X-ray diffraction and Raman spectroscopy revealed that PVDF crystallized predominantly as the γ-phase. Non-activated ZnO filler reduces the degree of the crystallinity of the matrix and promotes crystallization of α-phase of PVDF in the film, while the fillers activated for 5 and 10 min induce crystallization of β-phase, indicating that mechanical activation of the filler can be used as a general method for fabrication of PVDF composites with increased content of piezoelectric β-phase crystals. Dielectric spectroscopy measurements show that polymer composite with the high content of β-phase (with ZnO filler activated for 5 min) exhibits the highest value of dielectric permittivity in 150-400 K range of temperatures. Kinetic analysis shows combined effects of increased surface area and increased concentration of surface defects on the interactions between polymer chains and activated nanoparticles. © 2018 IOP Publishing Ltd.
T2  - Physica Scripta
T1  - Structural and electrical properties of ferroelectric poly(vinylidene fluoride) and mechanically activated ZnO nanoparticle composite films
VL  - 93
IS  - 10
SP  - 105801
DO  - 10.1088/1402-4896/aad749
ER  - 
@article{
author = "Peleš, Adriana and Aleksić, Obrad S. and Pavlović, Vera P. and Đoković, Vladimir and Dojčilović, Radovan and Nikolić, Zoran M. and Marinković, Filip S. and Mitrić, Miodrag and Blagojević, Vladimir D. and Vlahović, Branislav and Pavlović, Vladimir B.",
year = "2018",
abstract = "The influence of the mechanical activation of ZnO nanoparticle fillers on the structural and electrical properties of the matrix of poly(vinylidenefluoride)-ZnO (PVDF-ZnO) films was investigated. Transmission electron microscopy and scanning electron microscopy analyses showed that mechanical activation in a high energy planetary ball mill reduces the size of ZnO particles. X-ray diffraction and Raman spectroscopy revealed that PVDF crystallized predominantly as the γ-phase. Non-activated ZnO filler reduces the degree of the crystallinity of the matrix and promotes crystallization of α-phase of PVDF in the film, while the fillers activated for 5 and 10 min induce crystallization of β-phase, indicating that mechanical activation of the filler can be used as a general method for fabrication of PVDF composites with increased content of piezoelectric β-phase crystals. Dielectric spectroscopy measurements show that polymer composite with the high content of β-phase (with ZnO filler activated for 5 min) exhibits the highest value of dielectric permittivity in 150-400 K range of temperatures. Kinetic analysis shows combined effects of increased surface area and increased concentration of surface defects on the interactions between polymer chains and activated nanoparticles. © 2018 IOP Publishing Ltd.",
journal = "Physica Scripta",
title = "Structural and electrical properties of ferroelectric poly(vinylidene fluoride) and mechanically activated ZnO nanoparticle composite films",
volume = "93",
number = "10",
pages = "105801",
doi = "10.1088/1402-4896/aad749"
}
Peleš, A., Aleksić, O. S., Pavlović, V. P., Đoković, V., Dojčilović, R., Nikolić, Z. M., Marinković, F. S., Mitrić, M., Blagojević, V. D., Vlahović, B.,& Pavlović, V. B.. (2018). Structural and electrical properties of ferroelectric poly(vinylidene fluoride) and mechanically activated ZnO nanoparticle composite films. in Physica Scripta, 93(10), 105801.
https://doi.org/10.1088/1402-4896/aad749
Peleš A, Aleksić OS, Pavlović VP, Đoković V, Dojčilović R, Nikolić ZM, Marinković FS, Mitrić M, Blagojević VD, Vlahović B, Pavlović VB. Structural and electrical properties of ferroelectric poly(vinylidene fluoride) and mechanically activated ZnO nanoparticle composite films. in Physica Scripta. 2018;93(10):105801.
doi:10.1088/1402-4896/aad749 .
Peleš, Adriana, Aleksić, Obrad S., Pavlović, Vera P., Đoković, Vladimir, Dojčilović, Radovan, Nikolić, Zoran M., Marinković, Filip S., Mitrić, Miodrag, Blagojević, Vladimir D., Vlahović, Branislav, Pavlović, Vladimir B., "Structural and electrical properties of ferroelectric poly(vinylidene fluoride) and mechanically activated ZnO nanoparticle composite films" in Physica Scripta, 93, no. 10 (2018):105801,
https://doi.org/10.1088/1402-4896/aad749 . .
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