TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
AU  - Korićanac, Lela
AU  - Valastro, Lucia M.
AU  - Privitera, Giuseppe
AU  - Cuttone, Giacomo
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3613
AB  - The correlation between time dependent viabilities, after applying two radiation qualities and two alkylating agents on HTB140 melanoma cells, has been studied. Irradiations were performed with gamma-rays and 62 MeV protons, close to the Bragg peak maximum, delivering doses of 8-24 Gy. Treatments with fotemustine (FM) and dacarbazine (DTIC) were carried out with concentrations of 0.05-2 mM. High radio-resistance of HTB140 cells revealed by a clonogenic assay was confirmed by microtetrasolium and sulforhodamine B, through the surviving fraction at 2 Gy (SF2), being 0.961-0.956 for gamma-rays and 0.931-0.887 for protons. A better efficiency of protons was illustrated by relative biological effectiveness at 2 Gy (RBE), ranging from 1.69 to 1.89. A kinetic study of concentration dependent cytotoxicity indicated that the best effect of the drugs, estimated as the concentration that produces 50% of growth inhibition (IC(50)), was obtained at 48 h, having values of 76 mu M for DTIC and 145 mu M for FM. The cytostatic ability of the drugs pointed out that the presence of DTIC at 24 h, compared to FM, was insufficient to produce an effect. Protons and FM demonstrated their pro apoptotic capacity. Cross-resistance between treatments applied to the HTB140 cells was observed, protons being the most efficient, while DTIC, FM and gamma-rays demonstrated a lower level of cell inactivation. (C) 2008 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. ALL rights reserved.
T2  - Physica Medica
T1  - Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line
VL  - 24
IS  - 4
SP  - 187
EP  - 195
DO  - 10.1016/j.ejmp.2008.04.002
ER  - 

@article{
author = "Ristić-Fira, Aleksandra and Petrović, Ivan M. and Korićanac, Lela and Valastro, Lucia M. and Privitera, Giuseppe and Cuttone, Giacomo",
year = "2008",
abstract = "The correlation between time dependent viabilities, after applying two radiation qualities and two alkylating agents on HTB140 melanoma cells, has been studied. Irradiations were performed with gamma-rays and 62 MeV protons, close to the Bragg peak maximum, delivering doses of 8-24 Gy. Treatments with fotemustine (FM) and dacarbazine (DTIC) were carried out with concentrations of 0.05-2 mM. High radio-resistance of HTB140 cells revealed by a clonogenic assay was confirmed by microtetrasolium and sulforhodamine B, through the surviving fraction at 2 Gy (SF2), being 0.961-0.956 for gamma-rays and 0.931-0.887 for protons. A better efficiency of protons was illustrated by relative biological effectiveness at 2 Gy (RBE), ranging from 1.69 to 1.89. A kinetic study of concentration dependent cytotoxicity indicated that the best effect of the drugs, estimated as the concentration that produces 50% of growth inhibition (IC(50)), was obtained at 48 h, having values of 76 mu M for DTIC and 145 mu M for FM. The cytostatic ability of the drugs pointed out that the presence of DTIC at 24 h, compared to FM, was insufficient to produce an effect. Protons and FM demonstrated their pro apoptotic capacity. Cross-resistance between treatments applied to the HTB140 cells was observed, protons being the most efficient, while DTIC, FM and gamma-rays demonstrated a lower level of cell inactivation. (C) 2008 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. ALL rights reserved.",
journal = "Physica Medica",
title = "Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line",
volume = "24",
number = "4",
pages = "187-195",
doi = "10.1016/j.ejmp.2008.04.002"
}

Ristić-Fira, A., Petrović, I. M., Korićanac, L., Valastro, L. M., Privitera, G.,& Cuttone, G.. (2008). Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line. in Physica Medica, 24(4), 187-195.
https://doi.org/10.1016/j.ejmp.2008.04.002

Ristić-Fira A, Petrović IM, Korićanac L, Valastro LM, Privitera G, Cuttone G. Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line. in Physica Medica. 2008;24(4):187-195.
doi:10.1016/j.ejmp.2008.04.002 .

Ristić-Fira, Aleksandra, Petrović, Ivan M., Korićanac, Lela, Valastro, Lucia M., Privitera, Giuseppe, Cuttone, Giacomo, "Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line" in Physica Medica, 24, no. 4 (2008):187-195,
https://doi.org/10.1016/j.ejmp.2008.04.002 . .