Studies of enzyme interactions with toxic and pharmacologically active molecules

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Studies of enzyme interactions with toxic and pharmacologically active molecules (en)
Истраживања интеракција ензима са токсичним и фармаколошки активним молекулима (sr)
Istraživanja interakcija enzima sa toksičnim i farmakološki aktivnim molekulima (sr_RS)
Authors

Publications

Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents

Milojkov, Dušan V.; Silvestre, Oscar F.; Stanić, Vojislav; Janjić, Goran V.; Mutavdžić, Dragosav R.; Milanović, Marija M.; Nieder, Jana B.

(2020)

TY  - JOUR
AU  - Milojkov, Dušan V.
AU  - Silvestre, Oscar F.
AU  - Stanić, Vojislav
AU  - Janjić, Goran V.
AU  - Mutavdžić, Dragosav R.
AU  - Milanović, Marija M.
AU  - Nieder, Jana B.
PY  - 2020
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8529
AB  - Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiplet states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (3P0→3H5 transition of Pr3+ ion) and orange emission at 600 nm (1D2→3H4), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications. © 2019
T2  - Journal of Luminescence
T1  - Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents
VL  - 217
SP  - 116757
DO  - 10.1016/j.jlumin.2019.116757
ER  - 
@article{
author = "Milojkov, Dušan V. and Silvestre, Oscar F. and Stanić, Vojislav and Janjić, Goran V. and Mutavdžić, Dragosav R. and Milanović, Marija M. and Nieder, Jana B.",
year = "2020",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8529",
abstract = "Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiplet states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (3P0→3H5 transition of Pr3+ ion) and orange emission at 600 nm (1D2→3H4), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications. © 2019",
journal = "Journal of Luminescence",
title = "Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents",
volume = "217",
pages = "116757",
doi = "10.1016/j.jlumin.2019.116757"
}
Milojkov, D. V., Silvestre, O. F., Stanić, V., Janjić, G. V., Mutavdžić, D. R., Milanović, M. M.,& Nieder, J. B. (2020). Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents.
Journal of Luminescence, 217, 116757.
https://doi.org/10.1016/j.jlumin.2019.116757
Milojkov DV, Silvestre OF, Stanić V, Janjić GV, Mutavdžić DR, Milanović MM, Nieder JB. Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents. Journal of Luminescence. 2020;217:116757
Milojkov Dušan V., Silvestre Oscar F., Stanić Vojislav, Janjić Goran V., Mutavdžić Dragosav R., Milanović Marija M., Nieder Jana B., "Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents" Journal of Luminescence, 217 (2020):116757,
https://doi.org/10.1016/j.jlumin.2019.116757 .
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Comparison of fine particulate matter level, chemical content and oxidative potential derived from two dissimilar urban environments

Jovanović, Maja; Savić, Jasmina; Kovačević, Renata; Tasić, Viša; Todorović, Žaklina; Stevanović, Svetlana; Manojlović, Dragan D.; Jovašević-Stojanović, Milena

(2020)

TY  - JOUR
AU  - Jovanović, Maja
AU  - Savić, Jasmina
AU  - Kovačević, Renata
AU  - Tasić, Viša
AU  - Todorović, Žaklina
AU  - Stevanović, Svetlana
AU  - Manojlović, Dragan D.
AU  - Jovašević-Stojanović, Milena
PY  - 2020
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3820
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8750
AB  - Urban airborne particles contain a wide spectrum of components, known to have harmful effects on human health. This study reports a detailed investigation of fine particulate matter (PM2.5), chemical content and oxidative potential derived from two different urban environments. During summer and winter, 20-day campaigns were conducted at Belgrade city center (urban-background site – UB) and Bor (urban-industrial site – UI). Using various analytical techniques, carbonaceous compounds, water-soluble inorganic ions, major and trace elements were determined, while the oxidative potential of PM2.5 was estimated by dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay (OPDCFH values). The mean PM2.5 concentrations in both urban environments were above the recommended daily value, and the dominant PM2.5 mass contributor was organic matter (29–55%). The OC/EC ratio was significantly higher at UB site during winter, which was an indication of a considerable contribution of secondary organic carbon to the overall organic carbon (OC). Water-soluble organic carbon (WSOC) was also higher at UB than at UI site, and it probably came from the same sources as OC. In general, the different partition of secondary organic aerosol (SOA) in warm and cold periods affected the number of organic components. Sulfates and nitrates were the most abundant ions at both sites and they counted approximately 40% (summer) and 50% (winter) of total ions. Further, the concentrations of the most elements, particularly some potentially carcinogenic elements such as As, Cd and Pb were significantly higher at UI, due to the emissions from the copper smelter complex in the vicinity. The mean OPDCFH values were similar during the summer at both sampling sites, whereas a statistically significant difference between sites was noticed in favor of UB environment in winter.
T2  - Science of the Total Environment
T1  - Comparison of fine particulate matter level, chemical content and oxidative potential derived from two dissimilar urban environments
VL  - 708
SP  - 135209
DO  - 10.1016/j.scitotenv.2019.135209
ER  - 
@article{
author = "Jovanović, Maja and Savić, Jasmina and Kovačević, Renata and Tasić, Viša and Todorović, Žaklina and Stevanović, Svetlana and Manojlović, Dragan D. and Jovašević-Stojanović, Milena",
year = "2020",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3820, http://vinar.vin.bg.ac.rs/handle/123456789/8750",
abstract = "Urban airborne particles contain a wide spectrum of components, known to have harmful effects on human health. This study reports a detailed investigation of fine particulate matter (PM2.5), chemical content and oxidative potential derived from two different urban environments. During summer and winter, 20-day campaigns were conducted at Belgrade city center (urban-background site – UB) and Bor (urban-industrial site – UI). Using various analytical techniques, carbonaceous compounds, water-soluble inorganic ions, major and trace elements were determined, while the oxidative potential of PM2.5 was estimated by dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay (OPDCFH values). The mean PM2.5 concentrations in both urban environments were above the recommended daily value, and the dominant PM2.5 mass contributor was organic matter (29–55%). The OC/EC ratio was significantly higher at UB site during winter, which was an indication of a considerable contribution of secondary organic carbon to the overall organic carbon (OC). Water-soluble organic carbon (WSOC) was also higher at UB than at UI site, and it probably came from the same sources as OC. In general, the different partition of secondary organic aerosol (SOA) in warm and cold periods affected the number of organic components. Sulfates and nitrates were the most abundant ions at both sites and they counted approximately 40% (summer) and 50% (winter) of total ions. Further, the concentrations of the most elements, particularly some potentially carcinogenic elements such as As, Cd and Pb were significantly higher at UI, due to the emissions from the copper smelter complex in the vicinity. The mean OPDCFH values were similar during the summer at both sampling sites, whereas a statistically significant difference between sites was noticed in favor of UB environment in winter.",
journal = "Science of the Total Environment",
title = "Comparison of fine particulate matter level, chemical content and oxidative potential derived from two dissimilar urban environments",
volume = "708",
pages = "135209",
doi = "10.1016/j.scitotenv.2019.135209"
}
Jovanović, M., Savić, J., Kovačević, R., Tasić, V., Todorović, Ž., Stevanović, S., Manojlović, D. D.,& Jovašević-Stojanović, M. (2020). Comparison of fine particulate matter level, chemical content and oxidative potential derived from two dissimilar urban environments.
Science of the Total Environment, 708, 135209.
https://doi.org/10.1016/j.scitotenv.2019.135209
Jovanović M, Savić J, Kovačević R, Tasić V, Todorović Ž, Stevanović S, Manojlović DD, Jovašević-Stojanović M. Comparison of fine particulate matter level, chemical content and oxidative potential derived from two dissimilar urban environments. Science of the Total Environment. 2020;708:135209
Jovanović Maja, Savić Jasmina, Kovačević Renata, Tasić Viša, Todorović Žaklina, Stevanović Svetlana, Manojlović Dragan D., Jovašević-Stojanović Milena, "Comparison of fine particulate matter level, chemical content and oxidative potential derived from two dissimilar urban environments" Science of the Total Environment, 708 (2020):135209,
https://doi.org/10.1016/j.scitotenv.2019.135209 .
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Aminoalcoholate-driven tetracopper(II) cores as dual acetyl and butyrylcholinesterase inhibitors: Experimental and theoretical elucidation of mechanism of action

Bondžić, Aleksandra; Senćanski, Milan V.; Vujačić Nikezić, Ana V.; Kirillova, Marina V.; André, Vania; Kirillov, Alexander M.; Bondžić, Bojan P.

(2020)

TY  - JOUR
AU  - Bondžić, Aleksandra
AU  - Senćanski, Milan V.
AU  - Vujačić Nikezić, Ana V.
AU  - Kirillova, Marina V.
AU  - André, Vania
AU  - Kirillov, Alexander M.
AU  - Bondžić, Bojan P.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8806
AB  - Three coordination compounds featuring different types of tetracopper(II) cores, namely [O ⊂ Cu4N(CH2CH2O)34(BOH)4][BF4]2 (1), [Cu4(μ4-H2edte)(μ5-H2edte)(sal)2]n·7nH2O, (H4edte = N,N,N′,N′-tetrakis(2-hydroxyethyl)ethylenediamine, H2sal = salicylic acid) (2), and [Cu4(μ3-Hbes)4(μ-hba)K(H2O)3]n, H3bes = N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (3), were assayed for their potency to inhibit the acetyl (AChE) and butyrylcholinesterase (BuChE) enzymes aiming to test these compounds as potential dual inhibitors in the treatment of Alzheimer's disease. All the investigated compounds showed a strong inhibitory potency toward both enzymes with IC50 values in micromolar range of concentration; compound 1 displayed the most potent inhibitory behaviour toward both enzymes. The mechanism of the AChE and BuChE inhibition was examined by enzyme kinetic measurements. The obtained kinetic parameters, Vmax and Km indicated an uncompetitive type of inhibition of both enzymes by compound 1. For the other two compounds a non-competitive inhibition mode was observed. To get further insight into the mechanism of action and to elucidate binding modes in details we examined the interactions of 1–3 with acetylcholinesterase, using molecular docking approach. Grid based docking studies indicated that these compounds can bind to peripheral anionic site (PAS) of the AChE with Ki values in micromolar range. Moreover, blind docking revealed the capability of investigated compounds to bind to new allosteric site (i.e. binding site II) distinct from PAS. Showing that these Cu-based compounds can act as new allosteric inhibitors of AChE and identifying novel allosteric binding site on AChE represents a significant contribution toward the design of novel and more effective inhibitors of AChE. © 2020 Elsevier Inc.
T2  - Journal of Inorganic Biochemistry
T1  - Aminoalcoholate-driven tetracopper(II) cores as dual acetyl and butyrylcholinesterase inhibitors: Experimental and theoretical elucidation of mechanism of action
VL  - 205
SP  - 110990
DO  - 10.1016/j.jinorgbio.2019.110990
ER  - 
@article{
author = "Bondžić, Aleksandra and Senćanski, Milan V. and Vujačić Nikezić, Ana V. and Kirillova, Marina V. and André, Vania and Kirillov, Alexander M. and Bondžić, Bojan P.",
year = "2020",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/8806",
abstract = "Three coordination compounds featuring different types of tetracopper(II) cores, namely [O ⊂ Cu4N(CH2CH2O)34(BOH)4][BF4]2 (1), [Cu4(μ4-H2edte)(μ5-H2edte)(sal)2]n·7nH2O, (H4edte = N,N,N′,N′-tetrakis(2-hydroxyethyl)ethylenediamine, H2sal = salicylic acid) (2), and [Cu4(μ3-Hbes)4(μ-hba)K(H2O)3]n, H3bes = N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (3), were assayed for their potency to inhibit the acetyl (AChE) and butyrylcholinesterase (BuChE) enzymes aiming to test these compounds as potential dual inhibitors in the treatment of Alzheimer's disease. All the investigated compounds showed a strong inhibitory potency toward both enzymes with IC50 values in micromolar range of concentration; compound 1 displayed the most potent inhibitory behaviour toward both enzymes. The mechanism of the AChE and BuChE inhibition was examined by enzyme kinetic measurements. The obtained kinetic parameters, Vmax and Km indicated an uncompetitive type of inhibition of both enzymes by compound 1. For the other two compounds a non-competitive inhibition mode was observed. To get further insight into the mechanism of action and to elucidate binding modes in details we examined the interactions of 1–3 with acetylcholinesterase, using molecular docking approach. Grid based docking studies indicated that these compounds can bind to peripheral anionic site (PAS) of the AChE with Ki values in micromolar range. Moreover, blind docking revealed the capability of investigated compounds to bind to new allosteric site (i.e. binding site II) distinct from PAS. Showing that these Cu-based compounds can act as new allosteric inhibitors of AChE and identifying novel allosteric binding site on AChE represents a significant contribution toward the design of novel and more effective inhibitors of AChE. © 2020 Elsevier Inc.",
journal = "Journal of Inorganic Biochemistry",
title = "Aminoalcoholate-driven tetracopper(II) cores as dual acetyl and butyrylcholinesterase inhibitors: Experimental and theoretical elucidation of mechanism of action",
volume = "205",
pages = "110990",
doi = "10.1016/j.jinorgbio.2019.110990"
}
Bondžić, A., Senćanski, M. V., Vujačić Nikezić, A. V., Kirillova, M. V., André, V., Kirillov, A. M.,& Bondžić, B. P. (2020). Aminoalcoholate-driven tetracopper(II) cores as dual acetyl and butyrylcholinesterase inhibitors: Experimental and theoretical elucidation of mechanism of action.
Journal of Inorganic Biochemistry, 205, 110990.
https://doi.org/10.1016/j.jinorgbio.2019.110990
Bondžić A, Senćanski MV, Vujačić Nikezić AV, Kirillova MV, André V, Kirillov AM, Bondžić BP. Aminoalcoholate-driven tetracopper(II) cores as dual acetyl and butyrylcholinesterase inhibitors: Experimental and theoretical elucidation of mechanism of action. Journal of Inorganic Biochemistry. 2020;205:110990
Bondžić Aleksandra, Senćanski Milan V., Vujačić Nikezić Ana V., Kirillova Marina V., André Vania, Kirillov Alexander M., Bondžić Bojan P., "Aminoalcoholate-driven tetracopper(II) cores as dual acetyl and butyrylcholinesterase inhibitors: Experimental and theoretical elucidation of mechanism of action" Journal of Inorganic Biochemistry, 205 (2020):110990,
https://doi.org/10.1016/j.jinorgbio.2019.110990 .
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Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles

Laban, Bojana B.; Ralević, Uroš; Petrović, Sandra; Leskovac, Andreja; Vasić Anićijević, Dragana D.; Marković, Mirjana; Vasić, Vesna M.

(2020)

TY  - JOUR
AU  - Laban, Bojana B.
AU  - Ralević, Uroš
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Vasić Anićijević, Dragana D.
AU  - Marković, Mirjana
AU  - Vasić, Vesna M.
PY  - 2020
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8673
AB  - The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV–Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorption mode of L-Met on both Ag and Au surfaces was a vertical binding geometry via –NH2 group, while horizontal binding mode via [sbnd]S[sbnd] and –NH2 groups is also possible. The genotoxicity (evaluated by the micronucleus assay) of NPs, as well as their effects on some oxidative stress parameters (catalase activity, malondialdehyde level), were assessed in vitro using human peripheral blood cells as a model system. The influence of NPs on the morphology of lymphocyte cells studied using atomic force microscopy revealed that the membrane of cells remained unaffected after the treatment with NPs. When considering the effects of NPs on catalase activity and malondialdehyde level, neither particle type promoted oxidative stress. However, the treatment of lymphocytes with Ag@LM NPs induced a concentration-dependent enhancement of the micronuclei incidence and suppression of the cell proliferation while Au@LM NPs promoted cell proliferation, with no significant effects on micronuclei formation. The Ag@LM NPs were more prone to induce DNA damage than Au@LM NPs, which makes the latter type more suitable for further studies in nano-medicine. © 2019
T2  - Journal of Inorganic Biochemistry
T1  - Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles
VL  - 204
SP  - 110958
DO  - 10.1016/j.jinorgbio.2019.110958
ER  - 
@article{
author = "Laban, Bojana B. and Ralević, Uroš and Petrović, Sandra and Leskovac, Andreja and Vasić Anićijević, Dragana D. and Marković, Mirjana and Vasić, Vesna M.",
year = "2020",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8673",
abstract = "The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV–Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorption mode of L-Met on both Ag and Au surfaces was a vertical binding geometry via –NH2 group, while horizontal binding mode via [sbnd]S[sbnd] and –NH2 groups is also possible. The genotoxicity (evaluated by the micronucleus assay) of NPs, as well as their effects on some oxidative stress parameters (catalase activity, malondialdehyde level), were assessed in vitro using human peripheral blood cells as a model system. The influence of NPs on the morphology of lymphocyte cells studied using atomic force microscopy revealed that the membrane of cells remained unaffected after the treatment with NPs. When considering the effects of NPs on catalase activity and malondialdehyde level, neither particle type promoted oxidative stress. However, the treatment of lymphocytes with Ag@LM NPs induced a concentration-dependent enhancement of the micronuclei incidence and suppression of the cell proliferation while Au@LM NPs promoted cell proliferation, with no significant effects on micronuclei formation. The Ag@LM NPs were more prone to induce DNA damage than Au@LM NPs, which makes the latter type more suitable for further studies in nano-medicine. © 2019",
journal = "Journal of Inorganic Biochemistry",
title = "Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles",
volume = "204",
pages = "110958",
doi = "10.1016/j.jinorgbio.2019.110958"
}
Laban, B. B., Ralević, U., Petrović, S., Leskovac, A., Vasić Anićijević, D. D., Marković, M.,& Vasić, V. M. (2020). Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles.
Journal of Inorganic Biochemistry, 204, 110958.
https://doi.org/10.1016/j.jinorgbio.2019.110958
Laban BB, Ralević U, Petrović S, Leskovac A, Vasić Anićijević DD, Marković M, Vasić VM. Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles. Journal of Inorganic Biochemistry. 2020;204:110958
Laban Bojana B., Ralević Uroš, Petrović Sandra, Leskovac Andreja, Vasić Anićijević Dragana D., Marković Mirjana, Vasić Vesna M., "Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles" Journal of Inorganic Biochemistry, 204 (2020):110958,
https://doi.org/10.1016/j.jinorgbio.2019.110958 .
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In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system

Dinčić, Marko; Čolović, Mirjana B.; Sarić Matutinović, Marija; Ćetković, Mila; Kravić-Stevović, Tamara K.; Mougharbel, Ali S.; Todorović, Jasna; Ignjatović, Svetlana; Radosavljević, Branimir; Milisavljević, Milan; Kortz, Ulrich; Krstić, Danijela Z.

(2020)

TY  - JOUR
AU  - Dinčić, Marko
AU  - Čolović, Mirjana B.
AU  - Sarić Matutinović, Marija
AU  - Ćetković, Mila
AU  - Kravić-Stevović, Tamara K.
AU  - Mougharbel, Ali S.
AU  - Todorović, Jasna
AU  - Ignjatović, Svetlana
AU  - Radosavljević, Branimir
AU  - Milisavljević, Milan
AU  - Kortz, Ulrich
AU  - Krstić, Danijela Z.
PY  - 2020
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8479
AB  - In this study, thein vivohypoglycemic effect of a donut-shaped polyanion salt (NH4)14[Na@P5W30O110]$31H2O{NaP5W30} and its Ag-containing derivative K14[Ag@P5W30O110]$22H2O$6KCl {AgP5W30}, as wellas their hepatotoxicity and nephrotoxicity, was evaluated. In the screening hypoglycemic study,Wistaralbinorats with streptozotocin induced diabetes were treated intraperitoneally with three single doses (5,10, and 20 mg per kg per b.w.) of both investigated polyoxotungstates. The blood glucose levels,measured before and after 2, 4 and 6 h polyoxotungstate application, showed that both studiedcompounds induced the most pronounced and time dependent glucose lowering effects at the doses of20 mg kg1. Thus, daily doses of 20 mg kg1were administered toWistar albinorats orally for 14 days infurther toxicity examinations. The serum glucose concentration and biochemical parameters of kidneyand liver function, as well as a histopathological analysis of kidney and liver tissues were evaluated 14days after the polyoxotungstate administration. Both investigated compounds did not induce statisticallysignificant alterations of the serum glucose and uric acid concentrations, as well as some of the liverfunction markers (serum alanine and aspartate aminotransferases, and alkaline phosphatase activities).However, the significant decrease in serum total protein and albumin concentrations and the increase inbiochemical parameters of renal function–serum urea (up to 63.1%) and creatinine concentrations (upto 23.3%) were observed for both polyoxotungstates. In addition, the detected biochemical changeswere in accordance with kidney and liver histhopathological analysis. Accordingly, the hepatotoxic andnephrotoxic effects of these potential antidiabetic polyoxotungstates could be considered as mild.
T2  - RSC Advances
T1  - In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system
VL  - 10
IS  - 5
SP  - 2846
EP  - 2855
DO  - 10.1039/C9RA09790B
ER  - 
@article{
author = "Dinčić, Marko and Čolović, Mirjana B. and Sarić Matutinović, Marija and Ćetković, Mila and Kravić-Stevović, Tamara K. and Mougharbel, Ali S. and Todorović, Jasna and Ignjatović, Svetlana and Radosavljević, Branimir and Milisavljević, Milan and Kortz, Ulrich and Krstić, Danijela Z.",
year = "2020",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8479",
abstract = "In this study, thein vivohypoglycemic effect of a donut-shaped polyanion salt (NH4)14[Na@P5W30O110]$31H2O{NaP5W30} and its Ag-containing derivative K14[Ag@P5W30O110]$22H2O$6KCl {AgP5W30}, as wellas their hepatotoxicity and nephrotoxicity, was evaluated. In the screening hypoglycemic study,Wistaralbinorats with streptozotocin induced diabetes were treated intraperitoneally with three single doses (5,10, and 20 mg per kg per b.w.) of both investigated polyoxotungstates. The blood glucose levels,measured before and after 2, 4 and 6 h polyoxotungstate application, showed that both studiedcompounds induced the most pronounced and time dependent glucose lowering effects at the doses of20 mg kg1. Thus, daily doses of 20 mg kg1were administered toWistar albinorats orally for 14 days infurther toxicity examinations. The serum glucose concentration and biochemical parameters of kidneyand liver function, as well as a histopathological analysis of kidney and liver tissues were evaluated 14days after the polyoxotungstate administration. Both investigated compounds did not induce statisticallysignificant alterations of the serum glucose and uric acid concentrations, as well as some of the liverfunction markers (serum alanine and aspartate aminotransferases, and alkaline phosphatase activities).However, the significant decrease in serum total protein and albumin concentrations and the increase inbiochemical parameters of renal function–serum urea (up to 63.1%) and creatinine concentrations (upto 23.3%) were observed for both polyoxotungstates. In addition, the detected biochemical changeswere in accordance with kidney and liver histhopathological analysis. Accordingly, the hepatotoxic andnephrotoxic effects of these potential antidiabetic polyoxotungstates could be considered as mild.",
journal = "RSC Advances",
title = "In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system",
volume = "10",
number = "5",
pages = "2846-2855",
doi = "10.1039/C9RA09790B"
}
Dinčić, M., Čolović, M. B., Sarić Matutinović, M., Ćetković, M., Kravić-Stevović, T. K., Mougharbel, A. S., Todorović, J., Ignjatović, S., Radosavljević, B., Milisavljević, M., Kortz, U.,& Krstić, D. Z. (2020). In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system.
RSC Advances, 10(5), 2846-2855.
https://doi.org/10.1039/C9RA09790B
Dinčić M, Čolović MB, Sarić Matutinović M, Ćetković M, Kravić-Stevović TK, Mougharbel AS, Todorović J, Ignjatović S, Radosavljević B, Milisavljević M, Kortz U, Krstić DZ. In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system. RSC Advances. 2020;10(5):2846-2855
Dinčić Marko, Čolović Mirjana B., Sarić Matutinović Marija, Ćetković Mila, Kravić-Stevović Tamara K., Mougharbel Ali S., Todorović Jasna, Ignjatović Svetlana, Radosavljević Branimir, Milisavljević Milan, Kortz Ulrich, Krstić Danijela Z., "In vivo toxicity evaluation of two polyoxotungstates with potential antidiabetic activity using Wistar rats as a model system" RSC Advances, 10, no. 5 (2020):2846-2855,
https://doi.org/10.1039/C9RA09790B .
2
1

Atmospheric Solids Analysis Probe with Mass Spectrometry for Chlorpyrifos and Chlorpyrifos-Oxon Determination in Apples

Cvijović, Mirjana R.; Di Marco, Valerio; Stanković, Srboljub J.; Nedić, Zoran P.; Joksović, Ljubinka G.; Mihailović, Nevena R.

(2019)

TY  - JOUR
AU  - Cvijović, Mirjana R.
AU  - Di Marco, Valerio
AU  - Stanković, Srboljub J.
AU  - Nedić, Zoran P.
AU  - Joksović, Ljubinka G.
AU  - Mihailović, Nevena R.
PY  - 2019
UR  - https://journals.matheo.si/index.php/ACSi/article/view/4468
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8088
AB  - Chlorpyrifos (CPS) is a toxic pesticide present in several pesticide formulations, with low degradability by natural processes. The degradation leads to the toxic metabolite chlorpyrifos-oxon (CPO). The analytical techniques used for the CPS and CPO analysis, like UPLC-PDA and GC-MS, are accurate but also expensive and time consuming, and they need sample pretreatment. In the search of a more rapid and simple analytical procedure, atmospheric solids analysis probe with mass spectrometry (ASAP-MS) was optimized for the determination of CPS and CPO in apples (Malus domestica „Idared”). The identification of the analytes was based on protonated ion and isotopic pattern, while the quantification was based on peak intensities. The obtained results were confirmed by re-validated UPLC-PDA and GC-MS techniques. CPS and CPO concentrations determined by ASAP-MS and UPLC-PDA showed moderate discrepancies (on average by 10–20%), thus demonstrating that ASAP-MS can be a semiquantitative tool for the quantification of these compounds. As additional goal of this work, the efficiency of a gamma irradiation treatment to remove CPS and CPO from apples was tested by analyzing their content before and after the irradiation: 89–99% of CPS and CPO were degraded with doses of 3.5–3.8 kGy and 66–72 h of irradiation per sample. Identical degradation results were obtained by UPLC-PDA and ASAP-MS, indicating that the latter technique is well suitable to rapidly check pesticide degradation in apples. © 2019 Slovensko Kemijsko Drustvo. All Rights Reserved.
T2  - Acta Chimica Slovenica
T1  - Atmospheric Solids Analysis Probe with Mass Spectrometry for Chlorpyrifos and Chlorpyrifos-Oxon Determination in Apples
VL  - 66
IS  - 1
SP  - 70
EP  - 77
DO  - 10.17344/acsi.2018.4468
ER  - 
@article{
author = "Cvijović, Mirjana R. and Di Marco, Valerio and Stanković, Srboljub J. and Nedić, Zoran P. and Joksović, Ljubinka G. and Mihailović, Nevena R.",
year = "2019",
url = "https://journals.matheo.si/index.php/ACSi/article/view/4468, http://vinar.vin.bg.ac.rs/handle/123456789/8088",
abstract = "Chlorpyrifos (CPS) is a toxic pesticide present in several pesticide formulations, with low degradability by natural processes. The degradation leads to the toxic metabolite chlorpyrifos-oxon (CPO). The analytical techniques used for the CPS and CPO analysis, like UPLC-PDA and GC-MS, are accurate but also expensive and time consuming, and they need sample pretreatment. In the search of a more rapid and simple analytical procedure, atmospheric solids analysis probe with mass spectrometry (ASAP-MS) was optimized for the determination of CPS and CPO in apples (Malus domestica „Idared”). The identification of the analytes was based on protonated ion and isotopic pattern, while the quantification was based on peak intensities. The obtained results were confirmed by re-validated UPLC-PDA and GC-MS techniques. CPS and CPO concentrations determined by ASAP-MS and UPLC-PDA showed moderate discrepancies (on average by 10–20%), thus demonstrating that ASAP-MS can be a semiquantitative tool for the quantification of these compounds. As additional goal of this work, the efficiency of a gamma irradiation treatment to remove CPS and CPO from apples was tested by analyzing their content before and after the irradiation: 89–99% of CPS and CPO were degraded with doses of 3.5–3.8 kGy and 66–72 h of irradiation per sample. Identical degradation results were obtained by UPLC-PDA and ASAP-MS, indicating that the latter technique is well suitable to rapidly check pesticide degradation in apples. © 2019 Slovensko Kemijsko Drustvo. All Rights Reserved.",
journal = "Acta Chimica Slovenica",
title = "Atmospheric Solids Analysis Probe with Mass Spectrometry for Chlorpyrifos and Chlorpyrifos-Oxon Determination in Apples",
volume = "66",
number = "1",
pages = "70-77",
doi = "10.17344/acsi.2018.4468"
}
Cvijović, M. R., Di Marco, V., Stanković, S. J., Nedić, Z. P., Joksović, L. G.,& Mihailović, N. R. (2019). Atmospheric Solids Analysis Probe with Mass Spectrometry for Chlorpyrifos and Chlorpyrifos-Oxon Determination in Apples.
Acta Chimica Slovenica, 66(1), 70-77.
https://doi.org/10.17344/acsi.2018.4468
Cvijović MR, Di Marco V, Stanković SJ, Nedić ZP, Joksović LG, Mihailović NR. Atmospheric Solids Analysis Probe with Mass Spectrometry for Chlorpyrifos and Chlorpyrifos-Oxon Determination in Apples. Acta Chimica Slovenica. 2019;66(1):70-77
Cvijović Mirjana R., Di Marco Valerio, Stanković Srboljub J., Nedić Zoran P., Joksović Ljubinka G., Mihailović Nevena R., "Atmospheric Solids Analysis Probe with Mass Spectrometry for Chlorpyrifos and Chlorpyrifos-Oxon Determination in Apples" Acta Chimica Slovenica, 66, no. 1 (2019):70-77,
https://doi.org/10.17344/acsi.2018.4468 .
3
1
2

UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations

Savić, Jasmina; Petrović, Sandra; Leskovac, Andreja; Lazarević-Pašti, Tamara; Nastasijević, Branislav J.; Tanović, Brankica B.; Gašić, Slavica M.; Vasić, Vesna M.

(2019)

TY  - JOUR
AU  - Savić, Jasmina
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Lazarević-Pašti, Tamara
AU  - Nastasijević, Branislav J.
AU  - Tanović, Brankica B.
AU  - Gašić, Slavica M.
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814618313670
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7821
AB  - UV-C irradiation is widely used in the food industry. However, the health effects from dietary exposure to the irradiated pesticide residues retained in foodstuffs are underestimated. In this study, technical chlorpyrifos (TCPF) and its oil in water (EW) and emulsifiable concentrate (EC) formulations were irradiated by UV-C, and their photodegradation products were subjected to toxicity assessment, including determination of acetylcholinesterase (AChE) activity, genotoxicity and oxidative stress using human blood cells as a model system. Toxicity studies were performed using the chlorpyrifos concentrations in the range of those proposed as the maximum residue levels in plant commodities. TCPF, EW and EC photodegradation products induced DNA damage and oxidative stress, and their genotoxicity did not decrease as a function of irradiation time. Irradiated TCPF and EC are more potent AChE inhibitors than irradiated EW. Accordingly, the application of UV-C irradiation must be considered when processing the plants previously treated with chlorpyrifos formulations. © 2018 Elsevier Ltd
T2  - Food Chemistry
T1  - UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations
VL  - 271
SP  - 469
EP  - 478
DO  - 10.1016/j.foodchem.2018.07.207
ER  - 
@article{
author = "Savić, Jasmina and Petrović, Sandra and Leskovac, Andreja and Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Tanović, Brankica B. and Gašić, Slavica M. and Vasić, Vesna M.",
year = "2019",
url = "https://linkinghub.elsevier.com/retrieve/pii/S0308814618313670, http://vinar.vin.bg.ac.rs/handle/123456789/7821",
abstract = "UV-C irradiation is widely used in the food industry. However, the health effects from dietary exposure to the irradiated pesticide residues retained in foodstuffs are underestimated. In this study, technical chlorpyrifos (TCPF) and its oil in water (EW) and emulsifiable concentrate (EC) formulations were irradiated by UV-C, and their photodegradation products were subjected to toxicity assessment, including determination of acetylcholinesterase (AChE) activity, genotoxicity and oxidative stress using human blood cells as a model system. Toxicity studies were performed using the chlorpyrifos concentrations in the range of those proposed as the maximum residue levels in plant commodities. TCPF, EW and EC photodegradation products induced DNA damage and oxidative stress, and their genotoxicity did not decrease as a function of irradiation time. Irradiated TCPF and EC are more potent AChE inhibitors than irradiated EW. Accordingly, the application of UV-C irradiation must be considered when processing the plants previously treated with chlorpyrifos formulations. © 2018 Elsevier Ltd",
journal = "Food Chemistry",
title = "UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations",
volume = "271",
pages = "469-478",
doi = "10.1016/j.foodchem.2018.07.207"
}
Savić, J., Petrović, S., Leskovac, A., Lazarević-Pašti, T., Nastasijević, B. J., Tanović, B. B., Gašić, S. M.,& Vasić, V. M. (2019). UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations.
Food Chemistry, 271, 469-478.
https://doi.org/10.1016/j.foodchem.2018.07.207
Savić J, Petrović S, Leskovac A, Lazarević-Pašti T, Nastasijević BJ, Tanović BB, Gašić SM, Vasić VM. UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. Food Chemistry. 2019;271:469-478
Savić Jasmina, Petrović Sandra, Leskovac Andreja, Lazarević-Pašti Tamara, Nastasijević Branislav J., Tanović Brankica B., Gašić Slavica M., Vasić Vesna M., "UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations" Food Chemistry, 271 (2019):469-478,
https://doi.org/10.1016/j.foodchem.2018.07.207 .
8
6

Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models

Marković, Tatjana; Radanović, Dragoja; Nastasijević, Branislav J.; Antić-Mladenović, Svetlana; Vasić, Vesna M.; Matković, Ana

(2019)

TY  - JOUR
AU  - Marković, Tatjana
AU  - Radanović, Dragoja
AU  - Nastasijević, Branislav J.
AU  - Antić-Mladenović, Svetlana
AU  - Vasić, Vesna M.
AU  - Matković, Ana
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669019301190
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8073
AB  - Yellow gentian (Gentiana lutea ssp. symphyandra (Murb.) Hayek) is a high-mountainous perennial spontaneously growing on meadows and open slopes of the Eastern part of the Alps and the Balkan Peninsula. While the excessive exploitation endangered its survival in the nature in many European countries, the orientation to its large-scale production enables its protection at natural stands and satisfaction of increasing market demands for its roots, a highly-valuable herbal drug (Gentianae radix). During the six-year field trial established with Yellow gentian nursery plants on black water-air permeable and biodegradable film, in dry farming conditions of Mountain Tara (Serbia), the influence of a sigle basal application of farm yard manure and mineral fertilizer, at different planting densities (11.1, 13.3 and 16 plants m −2 ) on produced yield (roots), has been investigated. Single dose fertilization positively influenced the yields but did not provide optimal supplies for cultivated crop in the second part of experiment. It depended on crop age, planting density but also the climatic conditions. In the second part of plant production period, particulary in denser establishemnts, additional fertilization should be applied. Providing the appropriate amounts of fertilizers, Yellow gentian can be successfully grown even in the densest planting model (16 plants m −2 ), which is quite important in respect to the economic feasibility of cultivation. Similar yields achieved in mineral and organic treatments (4.51 kg m −2 and 4.85 kg m -2 of fresh root, respectively), suggest the roots might be successfully produced in both, conventional and organic cultivation model. In attempt to estimate impact of fertilization treatments on chemical quality of produced raw material (Gentianae radix) the content of several pharmacological constituents (loganic acid, swertiamarin, gentiopicroside, sweroside, amarogentin and isogentisin) was evaluated; gentiopicroside and loganic acid were the most dominant ones, regadless the fertilization, whereas the content of isogentisin was significantly increased in both, organic and mineral fertilization model, and content of sweroside, only in mineral model. With regard to the safety of produced raw material, the contents of biogenic (Fe, Mn, Zn, Cu) and non-biogenic (Pb) trace elements were analyzed; all trace elements were within the safty limits except the Cd content which slightly exceeded the limit. © 2019 Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models
VL  - 132
SP  - 236
EP  - 244
DO  - 10.1016/j.indcrop.2019.02.027
ER  - 
@article{
author = "Marković, Tatjana and Radanović, Dragoja and Nastasijević, Branislav J. and Antić-Mladenović, Svetlana and Vasić, Vesna M. and Matković, Ana",
year = "2019",
url = "https://linkinghub.elsevier.com/retrieve/pii/S0926669019301190, http://vinar.vin.bg.ac.rs/handle/123456789/8073",
abstract = "Yellow gentian (Gentiana lutea ssp. symphyandra (Murb.) Hayek) is a high-mountainous perennial spontaneously growing on meadows and open slopes of the Eastern part of the Alps and the Balkan Peninsula. While the excessive exploitation endangered its survival in the nature in many European countries, the orientation to its large-scale production enables its protection at natural stands and satisfaction of increasing market demands for its roots, a highly-valuable herbal drug (Gentianae radix). During the six-year field trial established with Yellow gentian nursery plants on black water-air permeable and biodegradable film, in dry farming conditions of Mountain Tara (Serbia), the influence of a sigle basal application of farm yard manure and mineral fertilizer, at different planting densities (11.1, 13.3 and 16 plants m −2 ) on produced yield (roots), has been investigated. Single dose fertilization positively influenced the yields but did not provide optimal supplies for cultivated crop in the second part of experiment. It depended on crop age, planting density but also the climatic conditions. In the second part of plant production period, particulary in denser establishemnts, additional fertilization should be applied. Providing the appropriate amounts of fertilizers, Yellow gentian can be successfully grown even in the densest planting model (16 plants m −2 ), which is quite important in respect to the economic feasibility of cultivation. Similar yields achieved in mineral and organic treatments (4.51 kg m −2 and 4.85 kg m -2 of fresh root, respectively), suggest the roots might be successfully produced in both, conventional and organic cultivation model. In attempt to estimate impact of fertilization treatments on chemical quality of produced raw material (Gentianae radix) the content of several pharmacological constituents (loganic acid, swertiamarin, gentiopicroside, sweroside, amarogentin and isogentisin) was evaluated; gentiopicroside and loganic acid were the most dominant ones, regadless the fertilization, whereas the content of isogentisin was significantly increased in both, organic and mineral fertilization model, and content of sweroside, only in mineral model. With regard to the safety of produced raw material, the contents of biogenic (Fe, Mn, Zn, Cu) and non-biogenic (Pb) trace elements were analyzed; all trace elements were within the safty limits except the Cd content which slightly exceeded the limit. © 2019 Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models",
volume = "132",
pages = "236-244",
doi = "10.1016/j.indcrop.2019.02.027"
}
Marković, T., Radanović, D., Nastasijević, B. J., Antić-Mladenović, S., Vasić, V. M.,& Matković, A. (2019). Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models.
Industrial Crops and Products, 132, 236-244.
https://doi.org/10.1016/j.indcrop.2019.02.027
Marković T, Radanović D, Nastasijević BJ, Antić-Mladenović S, Vasić VM, Matković A. Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models. Industrial Crops and Products. 2019;132:236-244
Marković Tatjana, Radanović Dragoja, Nastasijević Branislav J., Antić-Mladenović Svetlana, Vasić Vesna M., Matković Ana, "Yield, quality and safety of yellow gentian roots produced under dry-farming conditions in various single basal fertilization and planting density models" Industrial Crops and Products, 132 (2019):236-244,
https://doi.org/10.1016/j.indcrop.2019.02.027 .
3
2
2

Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays

Bošnjaković-Pavlović, Nada; Xu, Xiao; Krstić, Danijela Z.; Gillet, Jean-Michel; Wei, Yongge; Wu, Pingfan; Čolović, Mirjana B.; Spasojević-de Biré, Anne

(2019)

TY  - JOUR
AU  - Bošnjaković-Pavlović, Nada
AU  - Xu, Xiao
AU  - Krstić, Danijela Z.
AU  - Gillet, Jean-Michel
AU  - Wei, Yongge
AU  - Wu, Pingfan
AU  - Čolović, Mirjana B.
AU  - Spasojević-de Biré, Anne
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0162013418306998
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8355
AB  - The influence of three functionalized hexavanadates (V6): Na2 [V6O13{(OCH2)3CCH3}2], [H2]2 [V6O13{(OCH2)3CCH2OCOCH2CH3}2] and [(C4H9)4N]2 [V6O13{(OCH2)3CCH2OOC(CH3)2-COOH}2 on Na+/K+-ATPase activity, was investigated in vitro. Including compounds already tested by Xu et al. (Journal of Inorganic Biochemistry 161 (2016) 27–36), all functionalized hexavanadates inhibit the activity of Na+/K+-ATPase in a dose-dependent manner but with different inhibitory potencies. Na2 [V6O13{(OCH2)3CCH3}2] was found to have the best inhibition properties - showing 50% inhibition IC50 = 5.50 × 10−5 M, while [(C4H9)4N]2 [V6O13{(OCH2)3CCH2OOC(CH3)2-COOH}2] showed the lowest inhibitory power, IC50 = 1.31 × 10−4 M. In order to understand the bioactivity of functionalized hexavanadates series, we have also used a combined theoretical approach: determination of electrostatic potential from ab initio theoretical calculations and computation of the molecular interaction field (MIF) surface. © 2019
T2  - Journal of Inorganic Biochemistry
T1  - Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays
VL  - 198
SP  - 110720
DO  - 10.1016/j.jinorgbio.2019.110720
ER  - 
@article{
author = "Bošnjaković-Pavlović, Nada and Xu, Xiao and Krstić, Danijela Z. and Gillet, Jean-Michel and Wei, Yongge and Wu, Pingfan and Čolović, Mirjana B. and Spasojević-de Biré, Anne",
year = "2019",
url = "https://linkinghub.elsevier.com/retrieve/pii/S0162013418306998, http://vinar.vin.bg.ac.rs/handle/123456789/8355",
abstract = "The influence of three functionalized hexavanadates (V6): Na2 [V6O13{(OCH2)3CCH3}2], [H2]2 [V6O13{(OCH2)3CCH2OCOCH2CH3}2] and [(C4H9)4N]2 [V6O13{(OCH2)3CCH2OOC(CH3)2-COOH}2 on Na+/K+-ATPase activity, was investigated in vitro. Including compounds already tested by Xu et al. (Journal of Inorganic Biochemistry 161 (2016) 27–36), all functionalized hexavanadates inhibit the activity of Na+/K+-ATPase in a dose-dependent manner but with different inhibitory potencies. Na2 [V6O13{(OCH2)3CCH3}2] was found to have the best inhibition properties - showing 50% inhibition IC50 = 5.50 × 10−5 M, while [(C4H9)4N]2 [V6O13{(OCH2)3CCH2OOC(CH3)2-COOH}2] showed the lowest inhibitory power, IC50 = 1.31 × 10−4 M. In order to understand the bioactivity of functionalized hexavanadates series, we have also used a combined theoretical approach: determination of electrostatic potential from ab initio theoretical calculations and computation of the molecular interaction field (MIF) surface. © 2019",
journal = "Journal of Inorganic Biochemistry",
title = "Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays",
volume = "198",
pages = "110720",
doi = "10.1016/j.jinorgbio.2019.110720"
}
Bošnjaković-Pavlović, N., Xu, X., Krstić, D. Z., Gillet, J., Wei, Y., Wu, P., Čolović, M. B.,& Spasojević-de Biré, A. (2019). Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays.
Journal of Inorganic Biochemistry, 198, 110720.
https://doi.org/10.1016/j.jinorgbio.2019.110720
Bošnjaković-Pavlović N, Xu X, Krstić DZ, Gillet J, Wei Y, Wu P, Čolović MB, Spasojević-de Biré A. Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays. Journal of Inorganic Biochemistry. 2019;198:110720
Bošnjaković-Pavlović Nada, Xu Xiao, Krstić Danijela Z., Gillet Jean-Michel, Wei Yongge, Wu Pingfan, Čolović Mirjana B., Spasojević-de Biré Anne, "Experimental and theoretical insights of functionalized hexavanadates on Na+/K+-ATPase activity; molecular interaction field, ab initio calculations and in vitro assays" Journal of Inorganic Biochemistry, 198 (2019):110720,
https://doi.org/10.1016/j.jinorgbio.2019.110720 .
3
1
2

Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles

Bondžić, Aleksandra; Vujačić Nikezić, Ana V.; Klekotka, Urszula; Marković, Mirjana; Vodnik, Vesna; Kalska, B.; Vasić, Vesna M.

(2019)

TY  - JOUR
AU  - Bondžić, Aleksandra
AU  - Vujačić Nikezić, Ana V.
AU  - Klekotka,  Urszula
AU  - Marković, Mirjana
AU  - Vodnik, Vesna
AU  - Kalska, B.
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8812
AB  - Abstract: This paper presents the study of the interaction between gold nanoparticles (AuNPs) and antitumor gold complexes, [Au(OH)2(bipy)][PF6], [Au(CH3COO)2(pydmb-H)], and [Au(bipydmb-H)(OH)][PF6], in order to estimate the possibility for metal complex tracking in cells using nanospectroscopy approach. Decrease of intensity of the surface plasmon absorption band at 524 nm and the appearance of a new broad band at ∼640 nm, followed by their red shift were observed in the presence of the complexes. TEM images showed that the average size and shape of AuNPs did not change after the addition of gold complexes. DLS and zeta potential measurements pointed out to the metal complex adsorption on the surface of AuNPs followed by flocculation process. In addition, kinetic studies indicated strong bond formation between these complexes and AuNPs. Accordingly, AuNPs/complex conjugates have the potential to be applied for the tracking of these metal complexes in the cells using nanospectroscopy approach. © 2019, Pleiades Publishing, Ltd.
T2  - Russian Journal of Physical Chemistry A
T1  - Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles
VL  - 93
IS  - 13
SP  - 2765
EP  - 2770
DO  - 10.1134/S0036024419130065
ER  - 
@article{
author = "Bondžić, Aleksandra and Vujačić Nikezić, Ana V. and Klekotka,  Urszula and Marković, Mirjana and Vodnik, Vesna and Kalska, B. and Vasić, Vesna M.",
year = "2019",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/8812",
abstract = "Abstract: This paper presents the study of the interaction between gold nanoparticles (AuNPs) and antitumor gold complexes, [Au(OH)2(bipy)][PF6], [Au(CH3COO)2(pydmb-H)], and [Au(bipydmb-H)(OH)][PF6], in order to estimate the possibility for metal complex tracking in cells using nanospectroscopy approach. Decrease of intensity of the surface plasmon absorption band at 524 nm and the appearance of a new broad band at ∼640 nm, followed by their red shift were observed in the presence of the complexes. TEM images showed that the average size and shape of AuNPs did not change after the addition of gold complexes. DLS and zeta potential measurements pointed out to the metal complex adsorption on the surface of AuNPs followed by flocculation process. In addition, kinetic studies indicated strong bond formation between these complexes and AuNPs. Accordingly, AuNPs/complex conjugates have the potential to be applied for the tracking of these metal complexes in the cells using nanospectroscopy approach. © 2019, Pleiades Publishing, Ltd.",
journal = "Russian Journal of Physical Chemistry A",
title = "Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles",
volume = "93",
number = "13",
pages = "2765-2770",
doi = "10.1134/S0036024419130065"
}
Bondžić, A., Vujačić Nikezić, A. V., Klekotka, U., Marković, M., Vodnik, V., Kalska, B.,& Vasić, V. M. (2019). Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles.
Russian Journal of Physical Chemistry A, 93(13), 2765-2770.
https://doi.org/10.1134/S0036024419130065
Bondžić A, Vujačić Nikezić AV, Klekotka U, Marković M, Vodnik V, Kalska B, Vasić VM. Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles. Russian Journal of Physical Chemistry A. 2019;93(13):2765-2770
Bondžić Aleksandra, Vujačić Nikezić Ana V., Klekotka  Urszula, Marković Mirjana, Vodnik Vesna, Kalska B., Vasić Vesna M., "Insight into the Interaction between Selected Antitumor Gold(III) Complexes and Citrate Stabilized Gold Nanoparticles" Russian Journal of Physical Chemistry A, 93, no. 13 (2019):2765-2770,
https://doi.org/10.1134/S0036024419130065 .
1
1

Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue

Bondžić, Aleksandra; Leskovac, Andreja; Petrović, Sandra; Vasić Anićijević, Dragana D.; Luce, Marco; Massai, Lara; Generosi, Amanda; Paci, Barbara; Cricenti, Antonio; Messori, Luigi; Vasić, Vesna M.

(2019)

TY  - JOUR
AU  - Bondžić, Aleksandra
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Vasić Anićijević, Dragana D.
AU  - Luce, Marco
AU  - Massai, Lara
AU  - Generosi, Amanda
AU  - Paci, Barbara
AU  - Cricenti, Antonio
AU  - Messori, Luigi
AU  - Vasić, Vesna M.
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8747
AB  - Citrate-capped gold nanoparticles (AuNPs) were functionalized with three distinct antitumor gold(III) complexes, e.g., [Au(N,N)(OH)2][PF6], where (N,N)=2,2′-bipyridine; [Au(C,N)(AcO)2], where (C,N)=deprotonated 6-(1,1-dimethylbenzyl)-pyridine; [Au(C,N,N)(OH)][PF6], where (C,N,N)=deprotonated 6-(1,1-dimethylbenzyl)-2,2′-bipyridine, to assess the chance of tracking their subcellular distribution by atomic force microscopy (AFM), and surface enhanced Raman spectroscopy (SERS) techniques. An extensive physicochemical characterization of the formed conjugates was, thus, carried out by applying a variety of methods (density functional theory—DFT, UV/Vis spectrophotometry, AFM, Raman spectroscopy, and SERS). The resulting gold(III) complexes/AuNPs conjugates turned out to be pretty stable. Interestingly, they exhibited a dramatically increased resonance intensity in the Raman spectra induced by AuNPs. For testing the use of the functionalized AuNPs for biosensing, their distribution in the nuclear, cytosolic, and membrane cell fractions obtained from human lymphocytes was investigated by AFM and SERS. The conjugates were detected in the membrane and nuclear cell fractions but not in the cytosol. The AFM method confirmed that conjugates induced changes in the morphology and nanostructure of the membrane and nuclear fractions. The obtained results point out that the conjugates formed between AuNPs and gold(III) complexes may be used as a tool for tracking metallodrug distribution in the different cell fractions.
T2  - International Journal of Molecular Sciences
T1  - Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue
VL  - 20
IS  - 24
SP  - 6306
DO  - 10.3390/ijms20246306
ER  - 
@article{
author = "Bondžić, Aleksandra and Leskovac, Andreja and Petrović, Sandra and Vasić Anićijević, Dragana D. and Luce, Marco and Massai, Lara and Generosi, Amanda and Paci, Barbara and Cricenti, Antonio and Messori, Luigi and Vasić, Vesna M.",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8747",
abstract = "Citrate-capped gold nanoparticles (AuNPs) were functionalized with three distinct antitumor gold(III) complexes, e.g., [Au(N,N)(OH)2][PF6], where (N,N)=2,2′-bipyridine; [Au(C,N)(AcO)2], where (C,N)=deprotonated 6-(1,1-dimethylbenzyl)-pyridine; [Au(C,N,N)(OH)][PF6], where (C,N,N)=deprotonated 6-(1,1-dimethylbenzyl)-2,2′-bipyridine, to assess the chance of tracking their subcellular distribution by atomic force microscopy (AFM), and surface enhanced Raman spectroscopy (SERS) techniques. An extensive physicochemical characterization of the formed conjugates was, thus, carried out by applying a variety of methods (density functional theory—DFT, UV/Vis spectrophotometry, AFM, Raman spectroscopy, and SERS). The resulting gold(III) complexes/AuNPs conjugates turned out to be pretty stable. Interestingly, they exhibited a dramatically increased resonance intensity in the Raman spectra induced by AuNPs. For testing the use of the functionalized AuNPs for biosensing, their distribution in the nuclear, cytosolic, and membrane cell fractions obtained from human lymphocytes was investigated by AFM and SERS. The conjugates were detected in the membrane and nuclear cell fractions but not in the cytosol. The AFM method confirmed that conjugates induced changes in the morphology and nanostructure of the membrane and nuclear fractions. The obtained results point out that the conjugates formed between AuNPs and gold(III) complexes may be used as a tool for tracking metallodrug distribution in the different cell fractions.",
journal = "International Journal of Molecular Sciences",
title = "Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue",
volume = "20",
number = "24",
pages = "6306",
doi = "10.3390/ijms20246306"
}
Bondžić, A., Leskovac, A., Petrović, S., Vasić Anićijević, D. D., Luce, M., Massai, L., Generosi, A., Paci, B., Cricenti, A., Messori, L.,& Vasić, V. M. (2019). Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue.
International Journal of Molecular Sciences, 20(24), 6306.
https://doi.org/10.3390/ijms20246306
Bondžić A, Leskovac A, Petrović S, Vasić Anićijević DD, Luce M, Massai L, Generosi A, Paci B, Cricenti A, Messori L, Vasić VM. Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue. International Journal of Molecular Sciences. 2019;20(24):6306
Bondžić Aleksandra, Leskovac Andreja, Petrović Sandra, Vasić Anićijević Dragana D., Luce Marco, Massai Lara, Generosi Amanda, Paci Barbara, Cricenti Antonio, Messori Luigi, Vasić Vesna M., "Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue" International Journal of Molecular Sciences, 20, no. 24 (2019):6306,
https://doi.org/10.3390/ijms20246306 .

Measurements of Oxidative Potential of Particulate Matter at Belgrade Tunnel; Comparison of BPEAnit, DTT and DCFH Assays

Jovanović, Maja; Savić, Jasmina; Salimi, Farhad; Stevanović, Svetlana; Brown, Reece A.; Jovašević-Stojanović, Milena; Manojlovic, Dragan; Bartonova, Alena; Bottle, Steven; Ristovski, Zoran

(2019)

TY  - JOUR
AU  - Jovanović, Maja
AU  - Savić, Jasmina
AU  - Salimi, Farhad
AU  - Stevanović, Svetlana
AU  - Brown, Reece A.
AU  - Jovašević-Stojanović, Milena
AU  - Manojlovic, Dragan
AU  - Bartonova, Alena
AU  - Bottle, Steven
AU  - Ristovski, Zoran
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8677
AB  - To estimate the oxidative potential (OP) of particulate matter (PM), two commonly used cell-free, molecular probes were applied: dithiothreitol (DTT) and dichloro-dihydro-fluorescein diacetate (DCFH-DA), and their performance was compared with 9,10-bis (phenylethynyl) anthracene-nitroxide (BPEAnit). To the best of our knowledge, this is the first study in which the performance of the DTT and DCFH has been compared with the BPEAnit probe. The average concentrations of PM, organic carbon (OC) and elemental carbon (EC) for fine (PM2.5) and coarse (PM10) particles were determined. The results were 44.8 ± 13.7, 9.8 ± 5.1 and 9.3 ± 4.8 µg·m−3 for PM2.5 and 75.5 ± 25.1, 16.3 ± 8.7 and 11.8 ± 5.3 µg·m−3 for PM10, respectively, for PM, OC and EC. The water-soluble organic carbon (WSOC) fraction accounted for 42 ± 14% and 28 ± 9% of organic carbon in PM2.5 and PM10, respectively. The average volume normalized OP values for the three assays depended on both the sampling periods and the PM fractions. The OPBPEAnit had its peak at 2 p.m.; in the afternoon, it was three times higher compared to the morning and late afternoon values. The DCFH and BPEAnit results were correlated (r = 0.64), while there was no good agreement between the BPEAnit and the DTT (r = 0.14). The total organic content of PM does not necessarily represent oxidative capacity and it shows varying correlation with the OP. With respect to the two PM fractions studied, the OP was mostly associated with smaller particles.
T2  - International Journal of Environmental Research and Public Health
T1  - Measurements of Oxidative Potential of Particulate Matter at Belgrade Tunnel; Comparison of BPEAnit, DTT and DCFH Assays
VL  - 16
IS  - 24
SP  - 4906
DO  - 10.3390/ijerph16244906
ER  - 
@article{
author = "Jovanović, Maja and Savić, Jasmina and Salimi, Farhad and Stevanović, Svetlana and Brown, Reece A. and Jovašević-Stojanović, Milena and Manojlovic, Dragan and Bartonova, Alena and Bottle, Steven and Ristovski, Zoran",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8677",
abstract = "To estimate the oxidative potential (OP) of particulate matter (PM), two commonly used cell-free, molecular probes were applied: dithiothreitol (DTT) and dichloro-dihydro-fluorescein diacetate (DCFH-DA), and their performance was compared with 9,10-bis (phenylethynyl) anthracene-nitroxide (BPEAnit). To the best of our knowledge, this is the first study in which the performance of the DTT and DCFH has been compared with the BPEAnit probe. The average concentrations of PM, organic carbon (OC) and elemental carbon (EC) for fine (PM2.5) and coarse (PM10) particles were determined. The results were 44.8 ± 13.7, 9.8 ± 5.1 and 9.3 ± 4.8 µg·m−3 for PM2.5 and 75.5 ± 25.1, 16.3 ± 8.7 and 11.8 ± 5.3 µg·m−3 for PM10, respectively, for PM, OC and EC. The water-soluble organic carbon (WSOC) fraction accounted for 42 ± 14% and 28 ± 9% of organic carbon in PM2.5 and PM10, respectively. The average volume normalized OP values for the three assays depended on both the sampling periods and the PM fractions. The OPBPEAnit had its peak at 2 p.m.; in the afternoon, it was three times higher compared to the morning and late afternoon values. The DCFH and BPEAnit results were correlated (r = 0.64), while there was no good agreement between the BPEAnit and the DTT (r = 0.14). The total organic content of PM does not necessarily represent oxidative capacity and it shows varying correlation with the OP. With respect to the two PM fractions studied, the OP was mostly associated with smaller particles.",
journal = "International Journal of Environmental Research and Public Health",
title = "Measurements of Oxidative Potential of Particulate Matter at Belgrade Tunnel; Comparison of BPEAnit, DTT and DCFH Assays",
volume = "16",
number = "24",
pages = "4906",
doi = "10.3390/ijerph16244906"
}
Jovanović, M., Savić, J., Salimi, F., Stevanović, S., Brown, R. A., Jovašević-Stojanović, M., Manojlovic, D., Bartonova, A., Bottle, S.,& Ristovski, Z. (2019). Measurements of Oxidative Potential of Particulate Matter at Belgrade Tunnel; Comparison of BPEAnit, DTT and DCFH Assays.
International Journal of Environmental Research and Public Health, 16(24), 4906.
https://doi.org/10.3390/ijerph16244906
Jovanović M, Savić J, Salimi F, Stevanović S, Brown RA, Jovašević-Stojanović M, Manojlovic D, Bartonova A, Bottle S, Ristovski Z. Measurements of Oxidative Potential of Particulate Matter at Belgrade Tunnel; Comparison of BPEAnit, DTT and DCFH Assays. International Journal of Environmental Research and Public Health. 2019;16(24):4906
Jovanović Maja, Savić Jasmina, Salimi Farhad, Stevanović Svetlana, Brown Reece A., Jovašević-Stojanović Milena, Manojlovic Dragan, Bartonova Alena, Bottle Steven, Ristovski Zoran, "Measurements of Oxidative Potential of Particulate Matter at Belgrade Tunnel; Comparison of BPEAnit, DTT and DCFH Assays" International Journal of Environmental Research and Public Health, 16, no. 24 (2019):4906,
https://doi.org/10.3390/ijerph16244906 .
9
6
9

Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations

Savić, Jasmina; Petrović, Sandra; Leskovac, Andreja; Lazarević-Pašti, Tamara; Nastasijević, Branislav J.; Tanović, Brankica B.; Gašić, Slavica M.; Vasić, Vesna M.

(2019)

TY  - BOOK
AU  - Savić, Jasmina
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Lazarević-Pašti, Tamara
AU  - Nastasijević, Branislav J.
AU  - Tanović, Brankica B.
AU  - Gašić, Slavica M.
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814618313670
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7831
AB  - Supplementary data 1: Table 1S. The chromatographic gradient profile; Table 2S. CPF concentration decrease (corresponding initial CPF concentration decrease was set as 0%) for all three forms of CPF depending on irradiation time; Table 3S. CPF and CPO concentrations determined chromatographically for TCPF, EW and EC formulations, as the function of irradiation time; % of CPO comparing to initial CPF concentration in all three forms of CPF; 
Supplementary data 2: Material safety data sheet according to 1907/2006/EC, Article 31/version 1; 
Supplementary data 3: Material safety data sheet according to 1907/2006/EC, Article 31/version 4
T2  - Food Chemistry
T1  - Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations
VL  - 271
SP  - 469
EP  - 478
DO  - 10.1016/j.foodchem.2018.07.207
ER  - 
@book{
author = "Savić, Jasmina and Petrović, Sandra and Leskovac, Andreja and Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Tanović, Brankica B. and Gašić, Slavica M. and Vasić, Vesna M.",
year = "2019",
url = "https://linkinghub.elsevier.com/retrieve/pii/S0308814618313670, http://vinar.vin.bg.ac.rs/handle/123456789/7831",
abstract = "Supplementary data 1: Table 1S. The chromatographic gradient profile; Table 2S. CPF concentration decrease (corresponding initial CPF concentration decrease was set as 0%) for all three forms of CPF depending on irradiation time; Table 3S. CPF and CPO concentrations determined chromatographically for TCPF, EW and EC formulations, as the function of irradiation time; % of CPO comparing to initial CPF concentration in all three forms of CPF; 
Supplementary data 2: Material safety data sheet according to 1907/2006/EC, Article 31/version 1; 
Supplementary data 3: Material safety data sheet according to 1907/2006/EC, Article 31/version 4",
journal = "Food Chemistry",
title = "Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations",
volume = "271",
pages = "469-478",
doi = "10.1016/j.foodchem.2018.07.207"
}
Savić, J., Petrović, S., Leskovac, A., Lazarević-Pašti, T., Nastasijević, B. J., Tanović, B. B., Gašić, S. M.,& Vasić, V. M. (2019). Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations.
Food Chemistry, 271, 469-478.
https://doi.org/10.1016/j.foodchem.2018.07.207
Savić J, Petrović S, Leskovac A, Lazarević-Pašti T, Nastasijević BJ, Tanović BB, Gašić SM, Vasić VM. Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. Food Chemistry. 2019;271:469-478
Savić Jasmina, Petrović Sandra, Leskovac Andreja, Lazarević-Pašti Tamara, Nastasijević Branislav J., Tanović Brankica B., Gašić Slavica M., Vasić Vesna M., "Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations" Food Chemistry, 271 (2019):469-478,
https://doi.org/10.1016/j.foodchem.2018.07.207 .
8
6

Detection of Dimethoate Pesticide using Layer by Layer Deposition of PDAC/GO on Ag electrode

Ega, Tharun K; Al-Hamry, Ammar; Kanoun, Olfa; Lazarević-Pašti, Tamara; Bogdanović, Danica B.; Pašti, Igor A.; Rodriguez, Raul D.; Sheremet, Evgeniya; Paterno, Leonardo G.

(IEEE, 2019)

TY  - CONF
AU  - Ega, Tharun K
AU  - Al-Hamry, Ammar
AU  - Kanoun, Olfa
AU  - Lazarević-Pašti, Tamara
AU  - Bogdanović, Danica B.
AU  - Pašti, Igor A.
AU  - Rodriguez, Raul D.
AU  - Sheremet, Evgeniya
AU  - Paterno, Leonardo G.
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8665
AB  - Dimethoate (DMT) is an organophosphate pesticide (OP), which is widely used against insects and mites and their control in agriculture. As other OPs, DMT is also an inhibitor of acetylcholinesterase, which is responsible for the disabling of cholinesterase required for the functioning of the central nervous system. This pesticide can invade living cells of the human body through contact or ingestion. We report an electrochemical sensor based on a layer by layer deposition of PDAC/GO on silver electrodes. The sensor fabrication, physical characterization i.e. Raman spectroscopy and scanning electron microscopy of PDAC/GO based films, and its electrochemical characterization are discussed. The detection of DMT by analyzing electrochemical measurements including cyclic voltammetry and impedance spectroscopy shows that functionalization using layer by layer deposition improves electrochemical response and presents a basis for detection of DMT. The highest response is observed in the case of only one PDAC/GO layer which is attributed to the properly balanced interaction between DMT and PDAC/GO layer, and the increase of electrical resistivity of the PDAC/GO layer with its thickness. © 2019 IEEE.
PB  - IEEE
C3  - 2019 16th International Multi-Conference on Systems, Signals & Devices (SSD)
C3  - 2019 16th International Multi-Conference on Systems, Signals & Devices (SSD)
T1  - Detection of Dimethoate Pesticide using Layer by Layer Deposition of PDAC/GO on Ag electrode
SP  - 621
EP  - 625
DO  - 10.1109/SSD.2019.8893253
ER  - 
@conference{
author = "Ega, Tharun K and Al-Hamry, Ammar and Kanoun, Olfa and Lazarević-Pašti, Tamara and Bogdanović, Danica B. and Pašti, Igor A. and Rodriguez, Raul D. and Sheremet, Evgeniya and Paterno, Leonardo G.",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8665",
abstract = "Dimethoate (DMT) is an organophosphate pesticide (OP), which is widely used against insects and mites and their control in agriculture. As other OPs, DMT is also an inhibitor of acetylcholinesterase, which is responsible for the disabling of cholinesterase required for the functioning of the central nervous system. This pesticide can invade living cells of the human body through contact or ingestion. We report an electrochemical sensor based on a layer by layer deposition of PDAC/GO on silver electrodes. The sensor fabrication, physical characterization i.e. Raman spectroscopy and scanning electron microscopy of PDAC/GO based films, and its electrochemical characterization are discussed. The detection of DMT by analyzing electrochemical measurements including cyclic voltammetry and impedance spectroscopy shows that functionalization using layer by layer deposition improves electrochemical response and presents a basis for detection of DMT. The highest response is observed in the case of only one PDAC/GO layer which is attributed to the properly balanced interaction between DMT and PDAC/GO layer, and the increase of electrical resistivity of the PDAC/GO layer with its thickness. © 2019 IEEE.",
publisher = "IEEE",
journal = "2019 16th International Multi-Conference on Systems, Signals & Devices (SSD), 2019 16th International Multi-Conference on Systems, Signals & Devices (SSD)",
title = "Detection of Dimethoate Pesticide using Layer by Layer Deposition of PDAC/GO on Ag electrode",
pages = "621-625",
doi = "10.1109/SSD.2019.8893253"
}
Ega, T. K., Al-Hamry, A., Kanoun, O., Lazarević-Pašti, T., Bogdanović, D. B., Pašti, I. A., Rodriguez, R. D., Sheremet, E.,& Paterno, L. G. (2019). Detection of Dimethoate Pesticide using Layer by Layer Deposition of PDAC/GO on Ag electrode.
2019 16th International Multi-Conference on Systems, Signals & Devices (SSD)
IEEE., 621-625.
https://doi.org/10.1109/SSD.2019.8893253
Ega TK, Al-Hamry A, Kanoun O, Lazarević-Pašti T, Bogdanović DB, Pašti IA, Rodriguez RD, Sheremet E, Paterno LG. Detection of Dimethoate Pesticide using Layer by Layer Deposition of PDAC/GO on Ag electrode. 2019 16th International Multi-Conference on Systems, Signals & Devices (SSD). 2019;:621-625
Ega Tharun K, Al-Hamry Ammar, Kanoun Olfa, Lazarević-Pašti Tamara, Bogdanović Danica B., Pašti Igor A., Rodriguez Raul D., Sheremet Evgeniya, Paterno Leonardo G., "Detection of Dimethoate Pesticide using Layer by Layer Deposition of PDAC/GO on Ag electrode" 2019 16th International Multi-Conference on Systems, Signals & Devices (SSD) (2019):621-625,
https://doi.org/10.1109/SSD.2019.8893253 .
3
2

Non-thermal plasma needle as an effective tool in dimethoate removal from water

Mitrović, Tatjana; Lazović, Saša; Nastasijević, Branislav J.; Pašti, Igor A.; Vasić, Vesna M.; Lazarević-Pašti, Tamara

(2019)

TY  - JOUR
AU  - Mitrović, Tatjana
AU  - Lazović, Saša
AU  - Nastasijević, Branislav J.
AU  - Pašti, Igor A.
AU  - Vasić, Vesna M.
AU  - Lazarević-Pašti, Tamara
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0301479719307716
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8337
AB  - Intensive use of pesticides requires innovative approaches for their removal from the environment. Here we report the method for degradation of dimethoate in water using non-thermal plasma needle and analyze kinetics of dimethoate removal and possible degradation pathways. The effects of dimethoate initial concentration, plasma treatment time, Argon flow rate and the presence of radical promoters on the effectiveness of proposed method are evaluated. With argon flow rate of 0.5 slm (standard litres per minute) 1 × 10−4 M dimethoate can be removed within 30 min of treatment. Using UPLC analysis it was confirmed that one of the decomposition products is dimethoate oxo-analogue omethoate, which is in fact more toxic than dimethoate. However, the overall toxicity of contaminated water was reduced upon the treatment. The addition of H2O2 as a free radical promoter enhances dimethoate removal, while K2S2O8 results with selective conversion to omethoate. Using mass spectrometry in combination with the theoretical calculations, possible degradation pathways were proposed. The feasibility of the proposed method for dimethoate degradation in real water samples is confirmed. The proposed method is demonstrated as a highly effective approach for dimethoate removal without significant accumulation of undesirable toxic products and secondary waste. © 2019 Elsevier Ltd
T2  - Journal of Environmental Management
T1  - Non-thermal plasma needle as an effective tool in dimethoate removal from water
VL  - 246
SP  - 63
EP  - 70
DO  - 10.1016/j.jenvman.2019.05.143
ER  - 
@article{
author = "Mitrović, Tatjana and Lazović, Saša and Nastasijević, Branislav J. and Pašti, Igor A. and Vasić, Vesna M. and Lazarević-Pašti, Tamara",
year = "2019",
url = "https://linkinghub.elsevier.com/retrieve/pii/S0301479719307716, http://vinar.vin.bg.ac.rs/handle/123456789/8337",
abstract = "Intensive use of pesticides requires innovative approaches for their removal from the environment. Here we report the method for degradation of dimethoate in water using non-thermal plasma needle and analyze kinetics of dimethoate removal and possible degradation pathways. The effects of dimethoate initial concentration, plasma treatment time, Argon flow rate and the presence of radical promoters on the effectiveness of proposed method are evaluated. With argon flow rate of 0.5 slm (standard litres per minute) 1 × 10−4 M dimethoate can be removed within 30 min of treatment. Using UPLC analysis it was confirmed that one of the decomposition products is dimethoate oxo-analogue omethoate, which is in fact more toxic than dimethoate. However, the overall toxicity of contaminated water was reduced upon the treatment. The addition of H2O2 as a free radical promoter enhances dimethoate removal, while K2S2O8 results with selective conversion to omethoate. Using mass spectrometry in combination with the theoretical calculations, possible degradation pathways were proposed. The feasibility of the proposed method for dimethoate degradation in real water samples is confirmed. The proposed method is demonstrated as a highly effective approach for dimethoate removal without significant accumulation of undesirable toxic products and secondary waste. © 2019 Elsevier Ltd",
journal = "Journal of Environmental Management",
title = "Non-thermal plasma needle as an effective tool in dimethoate removal from water",
volume = "246",
pages = "63-70",
doi = "10.1016/j.jenvman.2019.05.143"
}
Mitrović, T., Lazović, S., Nastasijević, B. J., Pašti, I. A., Vasić, V. M.,& Lazarević-Pašti, T. (2019). Non-thermal plasma needle as an effective tool in dimethoate removal from water.
Journal of Environmental Management, 246, 63-70.
https://doi.org/10.1016/j.jenvman.2019.05.143
Mitrović T, Lazović S, Nastasijević BJ, Pašti IA, Vasić VM, Lazarević-Pašti T. Non-thermal plasma needle as an effective tool in dimethoate removal from water. Journal of Environmental Management. 2019;246:63-70
Mitrović Tatjana, Lazović Saša, Nastasijević Branislav J., Pašti Igor A., Vasić Vesna M., Lazarević-Pašti Tamara, "Non-thermal plasma needle as an effective tool in dimethoate removal from water" Journal of Environmental Management, 246 (2019):63-70,
https://doi.org/10.1016/j.jenvman.2019.05.143 .
7
5
6

Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites

Vujačić Nikezić, Ana V.; Janjić, Goran V.; Bondžić, Aleksandra; Zarić, Božidarka; Vasić Anićijević, Dragana D.; Momić, Tatjana; Vasić, Vesna M.

(2018)

TY  - JOUR
AU  - Vujačić Nikezić, Ana V.
AU  - Janjić, Goran V.
AU  - Bondžić, Aleksandra
AU  - Zarić, Božidarka
AU  - Vasić Anićijević, Dragana D.
AU  - Momić, Tatjana
AU  - Vasić, Vesna M.
PY  - 2018
UR  - http://xlink.rsc.org/?DOI=C8MT00111A
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7812
AB  - The present paper deals with investigation of the interaction between selected simple structure Au(iii) ([AuCl4]-, [AuCl2(dmso)2]+, [AuCl2(bipy)]+) and Pt(ii) ([PtCl2(dmso)2]) complexes with Na/K-ATPase as the target enzyme, using an experimental and theoretical approach. Reaction stoichiometries and binding constants for these enzyme/complex systems were determined, while kinetic measurements were used in order to reveal the type of inhibition. Based on the results obtained by quantum mechanical calculations (electrostatic surface potential (ESP), volume and surface of the complexes) the nature of the investigated complexes was characterized. By using the solvent accessible surface area (SASA) applied on specific inhibitory sites (ion channel and intracellular domains) the nature of these sites was described. Docking studies were used to determine the theoretical probability of the non-covalent metal binding site positions. Inhibition studies implied that all the investigated complexes decreased the activity of the enzyme while the kinetic analysis indicated an uncompetitive mode of inhibition for the selected complexes. Docking results suggested that the main inhibitory site of all these complexes is located in the ion translocation pathway on the extracellular side in the E2P enzyme conformation, similar to the case of cardiac glycosides, specific Na/K-ATPase inhibitors. Also, based on our knowledge, the hydrolyzed forms of [AuCl4]- and [PtCl2(dmso)2] complexes were investigated for the first time by theoretical calculations in this paper. Thereby, a new inhibitory site situated between the M2 and M4 helices was revealed. Binding in this site induces conformational changes in the enzyme domains and perturbs the E1-E2P conformational equilibrium, causing enzyme inhibition.
T2  - Metallomics
T1  - Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites
VL  - 10
IS  - 7
SP  - 1003
EP  - 1015
DO  - 10.1039/C8MT00111A
ER  - 
@article{
author = "Vujačić Nikezić, Ana V. and Janjić, Goran V. and Bondžić, Aleksandra and Zarić, Božidarka and Vasić Anićijević, Dragana D. and Momić, Tatjana and Vasić, Vesna M.",
year = "2018",
url = "http://xlink.rsc.org/?DOI=C8MT00111A, http://vinar.vin.bg.ac.rs/handle/123456789/7812",
abstract = "The present paper deals with investigation of the interaction between selected simple structure Au(iii) ([AuCl4]-, [AuCl2(dmso)2]+, [AuCl2(bipy)]+) and Pt(ii) ([PtCl2(dmso)2]) complexes with Na/K-ATPase as the target enzyme, using an experimental and theoretical approach. Reaction stoichiometries and binding constants for these enzyme/complex systems were determined, while kinetic measurements were used in order to reveal the type of inhibition. Based on the results obtained by quantum mechanical calculations (electrostatic surface potential (ESP), volume and surface of the complexes) the nature of the investigated complexes was characterized. By using the solvent accessible surface area (SASA) applied on specific inhibitory sites (ion channel and intracellular domains) the nature of these sites was described. Docking studies were used to determine the theoretical probability of the non-covalent metal binding site positions. Inhibition studies implied that all the investigated complexes decreased the activity of the enzyme while the kinetic analysis indicated an uncompetitive mode of inhibition for the selected complexes. Docking results suggested that the main inhibitory site of all these complexes is located in the ion translocation pathway on the extracellular side in the E2P enzyme conformation, similar to the case of cardiac glycosides, specific Na/K-ATPase inhibitors. Also, based on our knowledge, the hydrolyzed forms of [AuCl4]- and [PtCl2(dmso)2] complexes were investigated for the first time by theoretical calculations in this paper. Thereby, a new inhibitory site situated between the M2 and M4 helices was revealed. Binding in this site induces conformational changes in the enzyme domains and perturbs the E1-E2P conformational equilibrium, causing enzyme inhibition.",
journal = "Metallomics",
title = "Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites",
volume = "10",
number = "7",
pages = "1003-1015",
doi = "10.1039/C8MT00111A"
}
Vujačić Nikezić, A. V., Janjić, G. V., Bondžić, A., Zarić, B., Vasić Anićijević, D. D., Momić, T.,& Vasić, V. M. (2018). Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites.
Metallomics, 10(7), 1003-1015.
https://doi.org/10.1039/C8MT00111A
Vujačić Nikezić AV, Janjić GV, Bondžić A, Zarić B, Vasić Anićijević DD, Momić T, Vasić VM. Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites. Metallomics. 2018;10(7):1003-1015
Vujačić Nikezić Ana V., Janjić Goran V., Bondžić Aleksandra, Zarić Božidarka, Vasić Anićijević Dragana D., Momić Tatjana, Vasić Vesna M., "Interaction of Au(iii) and Pt(ii) complexes with Na/K-ATPase: experimental and theoretical study of reaction stoichiometry and binding sites" Metallomics, 10, no. 7 (2018):1003-1015,
https://doi.org/10.1039/C8MT00111A .
1
1
1

Nanospectroscopy of thiacyanine dye molecules adsorbed on silver nanoparticle clusters

Ralević, Uroš; Isic, Goran; Vasić Anićijević, Dragana D.; Laban, Bojana B.; Bogdanović, Una; Lazović, Vladimir M.; Vodnik, Vesna; Gajic, Rados

(2018)

TY  - JOUR
AU  - Ralević, Uroš
AU  - Isic, Goran
AU  - Vasić Anićijević, Dragana D.
AU  - Laban, Bojana B.
AU  - Bogdanović, Una
AU  - Lazović, Vladimir M.
AU  - Vodnik, Vesna
AU  - Gajic, Rados
PY  - 2018
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1890
AB  - The adsorption of thiacyanine dye molecules on citrate-stabilized silver nanoparticle clusters drop-cast onto freshly cleaved mica or highly oriented pyrolytic graphite surfaces is examined using colocalized surface-enhanced Raman spectroscopy and atomic force microscopy. The incidence of dye Raman signatures in photoluminescence hotspots identified around nanoparticle clusters is considered for both citrate- and borate-capped silver nanoparticles and found to be substantially lower in the former case, suggesting that the citrate anions impede the efficient dye adsorption. Rigorous numerical simulations of light scattering on random nanoparticle clusters are used for estimating the electromagnetic enhancement and elucidating the hotspot formation mechanism. The majority of the enhanced Raman signal, estimated to be more than 90%, is found to originate from the nanogaps between adjacent nanoparticles in the cluster, regardless of the cluster size and geometry. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Applied Surface Science
T1  - Nanospectroscopy of thiacyanine dye molecules adsorbed on silver nanoparticle clusters
VL  - 434
SP  - 540
EP  - 548
DO  - 10.1016/j.apsusc.2017.10.148
ER  - 
@article{
author = "Ralević, Uroš and Isic, Goran and Vasić Anićijević, Dragana D. and Laban, Bojana B. and Bogdanović, Una and Lazović, Vladimir M. and Vodnik, Vesna and Gajic, Rados",
year = "2018",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1890",
abstract = "The adsorption of thiacyanine dye molecules on citrate-stabilized silver nanoparticle clusters drop-cast onto freshly cleaved mica or highly oriented pyrolytic graphite surfaces is examined using colocalized surface-enhanced Raman spectroscopy and atomic force microscopy. The incidence of dye Raman signatures in photoluminescence hotspots identified around nanoparticle clusters is considered for both citrate- and borate-capped silver nanoparticles and found to be substantially lower in the former case, suggesting that the citrate anions impede the efficient dye adsorption. Rigorous numerical simulations of light scattering on random nanoparticle clusters are used for estimating the electromagnetic enhancement and elucidating the hotspot formation mechanism. The majority of the enhanced Raman signal, estimated to be more than 90%, is found to originate from the nanogaps between adjacent nanoparticles in the cluster, regardless of the cluster size and geometry. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Applied Surface Science",
title = "Nanospectroscopy of thiacyanine dye molecules adsorbed on silver nanoparticle clusters",
volume = "434",
pages = "540-548",
doi = "10.1016/j.apsusc.2017.10.148"
}
Ralević, U., Isic, G., Vasić Anićijević, D. D., Laban, B. B., Bogdanović, U., Lazović, V. M., Vodnik, V.,& Gajic, R. (2018). Nanospectroscopy of thiacyanine dye molecules adsorbed on silver nanoparticle clusters.
Applied Surface Science, 434, 540-548.
https://doi.org/10.1016/j.apsusc.2017.10.148
Ralević U, Isic G, Vasić Anićijević DD, Laban BB, Bogdanović U, Lazović VM, Vodnik V, Gajic R. Nanospectroscopy of thiacyanine dye molecules adsorbed on silver nanoparticle clusters. Applied Surface Science. 2018;434:540-548
Ralević Uroš, Isic Goran, Vasić Anićijević Dragana D., Laban Bojana B., Bogdanović Una, Lazović Vladimir M., Vodnik Vesna, Gajic Rados, "Nanospectroscopy of thiacyanine dye molecules adsorbed on silver nanoparticle clusters" Applied Surface Science, 434 (2018):540-548,
https://doi.org/10.1016/j.apsusc.2017.10.148 .
3
2
3
4

Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field

Dinčić, Marko; Krstić, Danijela Z.; Čolović, Mirjana B.; Nešović Ostojić, Jelena; Kovačević, Sanjin; De Luka, Silvio R.; Đorđević, Drago M.; Ćirković, Saša; Brkić, Predrag; Todorović, Jasna

(2018)

TY  - JOUR
AU  - Dinčić, Marko
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Nešović Ostojić, Jelena
AU  - Kovačević, Sanjin
AU  - De Luka, Silvio R.
AU  - Đorđević, Drago M.
AU  - Ćirković, Saša
AU  - Brkić, Predrag
AU  - Todorović, Jasna
PY  - 2018
UR  - https://www.tandfonline.com/doi/full/10.1080/09553002.2018.1518611
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7957
AB  - Purpose: It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and pathological processes, the modulation of Na+/K+-ATPase, ecto-ATPases and AChE activities, as well as oxidative stress responses were followed in synaptosomes isolated from rats after chronic exposure toward differently oriented SMF. Material and methods: Wistar albino rats were randomly divided into three experimental groups (six animals per group): Up and Down group - exposed to upward and downward oriented SMF, respectively, and Control group. After 50 days, the rats were sacrificed, and synaptosomes were isolated from the whole rat brain and used for testing the enzyme activities and oxidative stress parameters. Results: Chronic exposure to 1 mT SMF significantly increased ATPases, AChE activities, and malondialdehyde (MDA) level in both exposed groups, compared to control values. The significant decrease in synaptosomal catalase activity (1.48 ± 0.17 U/mg protein) induced by exposure to the downward oriented field, compared to those obtained for Control group (2.60 ± 0.29 U/mg protein), and Up group (2.72 ± 0.21 U/mg protein). Conclusions: It could be concluded that chronic exposure to differently oriented SMF increases ATPases and AChE activities in rat synaptosomes. Since brain ATPases and AChE have important roles in the pathogenesis of several neurological diseases, SMF influence on the activity of these enzymes may have potential therapeutic importance. © 2018, Copyright © 2018 Taylor & Francis Group, LLC.
T2  - International Journal of Radiation Biology
T1  - Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field
VL  - 94
IS  - 11
SP  - 1062
EP  - 1071
DO  - 10.1080/09553002.2018.1518611
ER  - 
@article{
author = "Dinčić, Marko and Krstić, Danijela Z. and Čolović, Mirjana B. and Nešović Ostojić, Jelena and Kovačević, Sanjin and De Luka, Silvio R. and Đorđević, Drago M. and Ćirković, Saša and Brkić, Predrag and Todorović, Jasna",
year = "2018",
url = "https://www.tandfonline.com/doi/full/10.1080/09553002.2018.1518611, http://vinar.vin.bg.ac.rs/handle/123456789/7957",
abstract = "Purpose: It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and pathological processes, the modulation of Na+/K+-ATPase, ecto-ATPases and AChE activities, as well as oxidative stress responses were followed in synaptosomes isolated from rats after chronic exposure toward differently oriented SMF. Material and methods: Wistar albino rats were randomly divided into three experimental groups (six animals per group): Up and Down group - exposed to upward and downward oriented SMF, respectively, and Control group. After 50 days, the rats were sacrificed, and synaptosomes were isolated from the whole rat brain and used for testing the enzyme activities and oxidative stress parameters. Results: Chronic exposure to 1 mT SMF significantly increased ATPases, AChE activities, and malondialdehyde (MDA) level in both exposed groups, compared to control values. The significant decrease in synaptosomal catalase activity (1.48 ± 0.17 U/mg protein) induced by exposure to the downward oriented field, compared to those obtained for Control group (2.60 ± 0.29 U/mg protein), and Up group (2.72 ± 0.21 U/mg protein). Conclusions: It could be concluded that chronic exposure to differently oriented SMF increases ATPases and AChE activities in rat synaptosomes. Since brain ATPases and AChE have important roles in the pathogenesis of several neurological diseases, SMF influence on the activity of these enzymes may have potential therapeutic importance. © 2018, Copyright © 2018 Taylor & Francis Group, LLC.",
journal = "International Journal of Radiation Biology",
title = "Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field",
volume = "94",
number = "11",
pages = "1062-1071",
doi = "10.1080/09553002.2018.1518611"
}
Dinčić, M., Krstić, D. Z., Čolović, M. B., Nešović Ostojić, J., Kovačević, S., De Luka, S. R., Đorđević, D. M., Ćirković, S., Brkić, P.,& Todorović, J. (2018). Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field.
International Journal of Radiation Biology, 94(11), 1062-1071.
https://doi.org/10.1080/09553002.2018.1518611
Dinčić M, Krstić DZ, Čolović MB, Nešović Ostojić J, Kovačević S, De Luka SR, Đorđević DM, Ćirković S, Brkić P, Todorović J. Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field. International Journal of Radiation Biology. 2018;94(11):1062-1071
Dinčić Marko, Krstić Danijela Z., Čolović Mirjana B., Nešović Ostojić Jelena, Kovačević Sanjin, De Luka Silvio R., Đorđević Drago M., Ćirković Saša, Brkić Predrag, Todorović Jasna, "Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field" International Journal of Radiation Biology, 94, no. 11 (2018):1062-1071,
https://doi.org/10.1080/09553002.2018.1518611 .
1
2
1
1

The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water

Lazarević-Pašti, Tamara; Anićijević, Vladan J.; Baljozović, Miloš; Vasić Anićijević, Dragana D.; Gutić, Sanjin J.; Vasić, Vesna M.; Skorodumova, Natalia V.; Pašti, Igor A.

(2018)

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Anićijević, Vladan J.
AU  - Baljozović, Miloš
AU  - Vasić Anićijević, Dragana D.
AU  - Gutić, Sanjin J.
AU  - Vasić, Vesna M.
AU  - Skorodumova, Natalia V.
AU  - Pašti, Igor A.
PY  - 2018
UR  - http://xlink.rsc.org/?DOI=C8EN00171E
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7730
AB  - The wide use of pesticides in modern agriculture and other areas results in an urgent need for their efficient removal from the environment. Adsorption of pesticides is one of the most commonly used strategies for this task. Here we analyze the adsorption of two organophosphorus pesticides, dimethoate (DMT) and chlorpyrifos (CPF), on graphene-based materials. The adsorption was found to be very sensitive to the structure of the adsorbents used. In particular, aliphatic DMT was found to prefer hydrophilic oxidized graphene surfaces. The CPF molecule, which contains an aromatic moiety, prefers adsorption on the surface of a graphene basal plane with high structural order and preserved π electron system. The toxicity of pesticide solutions is reduced after adsorption, suggesting that there is no oxidation of DMT and CPF to more toxic oxo forms. We emphasize that the combination of structural properties of adsorbents and adsorbates defines the adsorption of organophosphorus pesticides on graphene-based materials, while the specific surface area of adsorbents is not the major factor.
T2  - Environmental Science: Nano
T1  - The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water
VL  - 5
IS  - 6
SP  - 1482
EP  - 1494
DO  - 10.1039/C8EN00171E
ER  - 
@article{
author = "Lazarević-Pašti, Tamara and Anićijević, Vladan J. and Baljozović, Miloš and Vasić Anićijević, Dragana D. and Gutić, Sanjin J. and Vasić, Vesna M. and Skorodumova, Natalia V. and Pašti, Igor A.",
year = "2018",
url = "http://xlink.rsc.org/?DOI=C8EN00171E, http://vinar.vin.bg.ac.rs/handle/123456789/7730",
abstract = "The wide use of pesticides in modern agriculture and other areas results in an urgent need for their efficient removal from the environment. Adsorption of pesticides is one of the most commonly used strategies for this task. Here we analyze the adsorption of two organophosphorus pesticides, dimethoate (DMT) and chlorpyrifos (CPF), on graphene-based materials. The adsorption was found to be very sensitive to the structure of the adsorbents used. In particular, aliphatic DMT was found to prefer hydrophilic oxidized graphene surfaces. The CPF molecule, which contains an aromatic moiety, prefers adsorption on the surface of a graphene basal plane with high structural order and preserved π electron system. The toxicity of pesticide solutions is reduced after adsorption, suggesting that there is no oxidation of DMT and CPF to more toxic oxo forms. We emphasize that the combination of structural properties of adsorbents and adsorbates defines the adsorption of organophosphorus pesticides on graphene-based materials, while the specific surface area of adsorbents is not the major factor.",
journal = "Environmental Science: Nano",
title = "The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water",
volume = "5",
number = "6",
pages = "1482-1494",
doi = "10.1039/C8EN00171E"
}
Lazarević-Pašti, T., Anićijević, V. J., Baljozović, M., Vasić Anićijević, D. D., Gutić, S. J., Vasić, V. M., Skorodumova, N. V.,& Pašti, I. A. (2018). The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water.
Environmental Science: Nano, 5(6), 1482-1494.
https://doi.org/10.1039/C8EN00171E
Lazarević-Pašti T, Anićijević VJ, Baljozović M, Vasić Anićijević DD, Gutić SJ, Vasić VM, Skorodumova NV, Pašti IA. The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water. Environmental Science: Nano. 2018;5(6):1482-1494
Lazarević-Pašti Tamara, Anićijević Vladan J., Baljozović Miloš, Vasić Anićijević Dragana D., Gutić Sanjin J., Vasić Vesna M., Skorodumova Natalia V., Pašti Igor A., "The impact of the structure of graphene-based materials on the removal of organophosphorus pesticides from water" Environmental Science: Nano, 5, no. 6 (2018):1482-1494,
https://doi.org/10.1039/C8EN00171E .
7
27
15
25

Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents

Leskovac, Andreja; Petrović, Sandra; Lazarević-Pašti, Tamara; Krstić, Milena P.; Vasić, Vesna M.

(2018)

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Lazarević-Pašti, Tamara
AU  - Krstić, Milena P.
AU  - Vasić, Vesna M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s00775-018-1560-x
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7765
AB  - In recent years, the search for effective anticancer compounds based on transition metal complexes has been the focus of medical investigations. The synergy between the ruthenium(II) and N-alkylphenothiazine counter-ions (chlorpromazine hydrochloride, thioridazine hydrochloride and trifluoperazine dihydrochloride, respectively) through the formation of three different complexes (1–3) was investigated. We explored whether the selected counter-ions and complexes might affect redox homeostasis and genome integrity of normal human blood cells, and induce an inhibition of Na+/K+-ATPase and AChE at pharmacologically relevant doses. Our results have shown that counter-ions and complexes did not affect the activity of Na+/K+-ATPase, while AChE activity was inhibited in a dose-dependent manner. All investigated compounds disturbed the viability and redox homeostasis of lymphocytes. Complexes 1 and 2 displayed potent cytotoxic and prooxidant action while complex 3 behaved as a weaker genotoxic inducer. Still, the tested complexes appeared to be less genotoxic and more cytostatic than the corresponding counter-ions. The effects of selected complexes were also tested in PC12 and U2OS cancer cells with special attention being given to the ability of phenothiazines to affect dopamine D2 receptors. Using the confocal laser scanning microscopy, we observed that all the complexes reduced cell viability. Although all investigated complexes have been bound to the dopamine receptor D2-eGFP, only complex 3 reduced its surface density and increased its lateral mobility in investigated cell lines. Albeit the role of alternative targets for complex 3 cannot be ruled out, its effects should be further examined as potential treatment strategy against cancer cells that overexpress D2.
T2  - JBIC Journal of Biological Inorganic Chemistry
T1  - Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents
VL  - 23
IS  - 5
SP  - 689
EP  - 704
DO  - 10.1007/s00775-018-1560-x
ER  - 
@article{
author = "Leskovac, Andreja and Petrović, Sandra and Lazarević-Pašti, Tamara and Krstić, Milena P. and Vasić, Vesna M.",
year = "2018",
url = "http://link.springer.com/10.1007/s00775-018-1560-x, http://vinar.vin.bg.ac.rs/handle/123456789/7765",
abstract = "In recent years, the search for effective anticancer compounds based on transition metal complexes has been the focus of medical investigations. The synergy between the ruthenium(II) and N-alkylphenothiazine counter-ions (chlorpromazine hydrochloride, thioridazine hydrochloride and trifluoperazine dihydrochloride, respectively) through the formation of three different complexes (1–3) was investigated. We explored whether the selected counter-ions and complexes might affect redox homeostasis and genome integrity of normal human blood cells, and induce an inhibition of Na+/K+-ATPase and AChE at pharmacologically relevant doses. Our results have shown that counter-ions and complexes did not affect the activity of Na+/K+-ATPase, while AChE activity was inhibited in a dose-dependent manner. All investigated compounds disturbed the viability and redox homeostasis of lymphocytes. Complexes 1 and 2 displayed potent cytotoxic and prooxidant action while complex 3 behaved as a weaker genotoxic inducer. Still, the tested complexes appeared to be less genotoxic and more cytostatic than the corresponding counter-ions. The effects of selected complexes were also tested in PC12 and U2OS cancer cells with special attention being given to the ability of phenothiazines to affect dopamine D2 receptors. Using the confocal laser scanning microscopy, we observed that all the complexes reduced cell viability. Although all investigated complexes have been bound to the dopamine receptor D2-eGFP, only complex 3 reduced its surface density and increased its lateral mobility in investigated cell lines. Albeit the role of alternative targets for complex 3 cannot be ruled out, its effects should be further examined as potential treatment strategy against cancer cells that overexpress D2.",
journal = "JBIC Journal of Biological Inorganic Chemistry",
title = "Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents",
volume = "23",
number = "5",
pages = "689-704",
doi = "10.1007/s00775-018-1560-x"
}
Leskovac, A., Petrović, S., Lazarević-Pašti, T., Krstić, M. P.,& Vasić, V. M. (2018). Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents.
JBIC Journal of Biological Inorganic Chemistry, 23(5), 689-704.
https://doi.org/10.1007/s00775-018-1560-x
Leskovac A, Petrović S, Lazarević-Pašti T, Krstić MP, Vasić VM. Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents. JBIC Journal of Biological Inorganic Chemistry. 2018;23(5):689-704
Leskovac Andreja, Petrović Sandra, Lazarević-Pašti Tamara, Krstić Milena P., Vasić Vesna M., "Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents" JBIC Journal of Biological Inorganic Chemistry, 23, no. 5 (2018):689-704,
https://doi.org/10.1007/s00775-018-1560-x .

Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals

Čolović, Mirjana B.; Vasić, Vesna M.; Đurić, Dragan M.; Krstić, Danijela Z.

(2018)

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Vasić, Vesna M.
AU  - Đurić, Dragan M.
AU  - Krstić, Danijela Z.
PY  - 2018
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1926
AB  - Background: Sulphur is an abundant element in biological systems, which plays an important role in processes essential for life as a constituent of proteins, vitamins and other crucial biomolecules. The major source of sulphur for humans is plants being able to use inorganic sulphur in the purpose of sulphur-containing amino acids synthesis. Sulphur-containing amino acids include methionine, cysteine, homocysteine, and taurine. Methionine and cysteine are classified as proteinogenic, canonic amino acids incorporated in protein structure. Sulphur amino acids are involved in the synthesis of intracellular antioxidants such as glutathione and N-acetyl cysteine. Moreover, naturally occurring sulphur-containing ligands are effective and safe detoxifying agents, often used in order to prevent toxic metal ions effects and their accumulation in human body. Methods: Literature search for peer-reviewed articles was performed using PubMed and Scopus databases, and utilizing appropriate keywords. Results: This review is focused on sulphur-containing amino acids - methionine, cysteine, taurine, and their derivatives - glutathione and N-acetylcysteine, and their defense effects as antioxidant agents against free radicals. Additionally, the protective effects of sulphur-containing ligands against the toxic effects of heavy and transition metal ions, and their reactivation role towards the enzyme inhibition are described. Conclusion: Sulphur-containing amino acids represent a powerful part of cell antioxidant system. Thus, they are essential in the maintenance of normal cellular functions and health. In addition to their worthy antioxidant action, sulphur-containing amino acids may offer a chelating site for heavy metals. Accordingly, they may be supplemented during chelating therapy, providing beneficial effects in eliminating toxic metals.
T2  - Current Medicinal Chemistry
T1  - Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals
VL  - 25
IS  - 3
SP  - 324
EP  - 335
DO  - 10.2174/0929867324666170609075434
ER  - 
@article{
author = "Čolović, Mirjana B. and Vasić, Vesna M. and Đurić, Dragan M. and Krstić, Danijela Z.",
year = "2018",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1926",
abstract = "Background: Sulphur is an abundant element in biological systems, which plays an important role in processes essential for life as a constituent of proteins, vitamins and other crucial biomolecules. The major source of sulphur for humans is plants being able to use inorganic sulphur in the purpose of sulphur-containing amino acids synthesis. Sulphur-containing amino acids include methionine, cysteine, homocysteine, and taurine. Methionine and cysteine are classified as proteinogenic, canonic amino acids incorporated in protein structure. Sulphur amino acids are involved in the synthesis of intracellular antioxidants such as glutathione and N-acetyl cysteine. Moreover, naturally occurring sulphur-containing ligands are effective and safe detoxifying agents, often used in order to prevent toxic metal ions effects and their accumulation in human body. Methods: Literature search for peer-reviewed articles was performed using PubMed and Scopus databases, and utilizing appropriate keywords. Results: This review is focused on sulphur-containing amino acids - methionine, cysteine, taurine, and their derivatives - glutathione and N-acetylcysteine, and their defense effects as antioxidant agents against free radicals. Additionally, the protective effects of sulphur-containing ligands against the toxic effects of heavy and transition metal ions, and their reactivation role towards the enzyme inhibition are described. Conclusion: Sulphur-containing amino acids represent a powerful part of cell antioxidant system. Thus, they are essential in the maintenance of normal cellular functions and health. In addition to their worthy antioxidant action, sulphur-containing amino acids may offer a chelating site for heavy metals. Accordingly, they may be supplemented during chelating therapy, providing beneficial effects in eliminating toxic metals.",
journal = "Current Medicinal Chemistry",
title = "Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals",
volume = "25",
number = "3",
pages = "324-335",
doi = "10.2174/0929867324666170609075434"
}
Čolović, M. B., Vasić, V. M., Đurić, D. M.,& Krstić, D. Z. (2018). Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals.
Current Medicinal Chemistry, 25(3), 324-335.
https://doi.org/10.2174/0929867324666170609075434
Čolović MB, Vasić VM, Đurić DM, Krstić DZ. Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals. Current Medicinal Chemistry. 2018;25(3):324-335
Čolović Mirjana B., Vasić Vesna M., Đurić Dragan M., Krstić Danijela Z., "Sulphur-containing Amino Acids: Protective Role Against Free Radicals and Heavy Metals" Current Medicinal Chemistry, 25, no. 3 (2018):324-335,
https://doi.org/10.2174/0929867324666170609075434 .
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The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach

Bondžić, Aleksandra; Čolović, Mirjana B.; Janjić, Goran V.; Zarić, Božidarka; Petrović, Sandra; Krstić, Danijela Z.; Marzo, Tiziano; Messori, Luigi; Vasić, Vesna M.

(2017)

TY  - JOUR
AU  - Bondžić, Aleksandra
AU  - Čolović, Mirjana B.
AU  - Janjić, Goran V.
AU  - Zarić, Božidarka
AU  - Petrović, Sandra
AU  - Krstić, Danijela Z.
AU  - Marzo, Tiziano
AU  - Messori, Luigi
AU  - Vasić, Vesna M.
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1648
AB  - The in vitro effects of oxo-bridged binuclear gold(III) complexes, i.e., [(bipy2Me)(2)Au-2(mu-O)(2)][PF6](2) (Auoxo6), Au-2[(bipydmb-H)(2)(mu-O)][PF6] (Au(2)bipyC) and [Au-2(phen(2Me))(2)(mu-O)(2)](PF6)(2) (Au(2)phen) on Na/K-ATPase, purified from the porcine cerebral cortex, were investigated. All three studied gold complexes inhibited the enzyme activity in a concentration-dependent manner achieving IC50 values in the low micromolar range. Kinetic analysis suggested an uncompetitive mode of inhibition for Auoxo6 and Au(2)bipyC, and a mixed type one for Au(2)phen. Docking studies indicated that the inhibitory actions of all tested complexes are related to E2-P enzyme conformation binding to ion channel and intracellular part between N and P sub-domain. In addition, Au(2)phen was able to inhibit the enzyme by interacting with its extracellular part as well. Toxic effects of the gold(III) complexes were evaluated in vitro by following lactate dehydrogenase activity in rat brain synaptosomes and incidence of micronuclei and cytokinesis-block proliferation index in cultivated human lymphocytes. All investigated complexes turned out to induce cytogenetic damage consisting of a significant decrease in cell proliferation and an increase in micronuclei in a dose-dependent manner. On the other hand, lactate dehydrogenase activity, an indicator of membrane integrity/viability, was not affected by Auoxo6 and Au(2)bipyC, while Au(2)phen slightly modified its activity.
T2  - Journal of Biological Inorganic Chemistry
T1  - The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach
VL  - 22
IS  - 6
SP  - 819
EP  - 832
DO  - 10.1007/s00775-017-1460-5
ER  - 
@article{
author = "Bondžić, Aleksandra and Čolović, Mirjana B. and Janjić, Goran V. and Zarić, Božidarka and Petrović, Sandra and Krstić, Danijela Z. and Marzo, Tiziano and Messori, Luigi and Vasić, Vesna M.",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1648",
abstract = "The in vitro effects of oxo-bridged binuclear gold(III) complexes, i.e., [(bipy2Me)(2)Au-2(mu-O)(2)][PF6](2) (Auoxo6), Au-2[(bipydmb-H)(2)(mu-O)][PF6] (Au(2)bipyC) and [Au-2(phen(2Me))(2)(mu-O)(2)](PF6)(2) (Au(2)phen) on Na/K-ATPase, purified from the porcine cerebral cortex, were investigated. All three studied gold complexes inhibited the enzyme activity in a concentration-dependent manner achieving IC50 values in the low micromolar range. Kinetic analysis suggested an uncompetitive mode of inhibition for Auoxo6 and Au(2)bipyC, and a mixed type one for Au(2)phen. Docking studies indicated that the inhibitory actions of all tested complexes are related to E2-P enzyme conformation binding to ion channel and intracellular part between N and P sub-domain. In addition, Au(2)phen was able to inhibit the enzyme by interacting with its extracellular part as well. Toxic effects of the gold(III) complexes were evaluated in vitro by following lactate dehydrogenase activity in rat brain synaptosomes and incidence of micronuclei and cytokinesis-block proliferation index in cultivated human lymphocytes. All investigated complexes turned out to induce cytogenetic damage consisting of a significant decrease in cell proliferation and an increase in micronuclei in a dose-dependent manner. On the other hand, lactate dehydrogenase activity, an indicator of membrane integrity/viability, was not affected by Auoxo6 and Au(2)bipyC, while Au(2)phen slightly modified its activity.",
journal = "Journal of Biological Inorganic Chemistry",
title = "The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach",
volume = "22",
number = "6",
pages = "819-832",
doi = "10.1007/s00775-017-1460-5"
}
Bondžić, A., Čolović, M. B., Janjić, G. V., Zarić, B., Petrović, S., Krstić, D. Z., Marzo, T., Messori, L.,& Vasić, V. M. (2017). The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach.
Journal of Biological Inorganic Chemistry, 22(6), 819-832.
https://doi.org/10.1007/s00775-017-1460-5
Bondžić A, Čolović MB, Janjić GV, Zarić B, Petrović S, Krstić DZ, Marzo T, Messori L, Vasić VM. The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach. Journal of Biological Inorganic Chemistry. 2017;22(6):819-832
Bondžić Aleksandra, Čolović Mirjana B., Janjić Goran V., Zarić Božidarka, Petrović Sandra, Krstić Danijela Z., Marzo Tiziano, Messori Luigi, Vasić Vesna M., "The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach" Journal of Biological Inorganic Chemistry, 22, no. 6 (2017):819-832,
https://doi.org/10.1007/s00775-017-1460-5 .
3
3
3

Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity

Čolović, Mirjana B.; Medic, Branislava; Cetkovic, Mila; Kravić-Stevović, Tamara K.; Stojanovic, Marko; Ayass, Wassim W.; Mougharbel, Ali S.; Radenković, Miroslav; Prostran, Milica; Kortz, Ulrich; Krstić, Danijela Z.

(2017)

TY  - JOUR
AU  - Čolović, Mirjana B.
AU  - Medic, Branislava
AU  - Cetkovic, Mila
AU  - Kravić-Stevović, Tamara K.
AU  - Stojanovic, Marko
AU  - Ayass, Wassim W.
AU  - Mougharbel, Ali S.
AU  - Radenković, Miroslav
AU  - Prostran, Milica
AU  - Kortz, Ulrich
AU  - Krstić, Danijela Z.
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1748
AB  - A toxicity evaluation of two Keggin-type heteropolytungstates, K-7[Ti2PW10O40].6H(2)O and K6H [SiV3W9O40].3H(2)O, with different inhibitory potencies toward acetylcholinesterase activity (IC50 values of 1.04 x 10(-6) and 4.80 x 10(-4) mol/L, respectively) was performed. Wistar albino rats were orally treated with single doses (5 and 50 mg/kg) of both investigated compounds. The biochemical parameters of renal (serum urea and creatinine) and liver function (direct and total bilirubin, alanine transaminase, and aspartate aminotransferase) were determined after 24 h and 14 days. A histopathological analysis of liver tissue was carried out 14 days after the polyoxotungstate administration. Both applied doses of the investigated compounds did not induce statistically significant alterations of the renal function markers. However, the polyoxotungstate treatment caused an increase in the activities of serum alanine transaminase and aspartate aminotransferase in a time- and concentration -dependent manner, although statistically significant changes in bilirubin concentrations were not observed. Furthermore, the detected hepatotoxic effect was confirmed by histhopathological analysis that suggested some reversible liver tissue damage two weeks after the treatment, especially in the case of K6H [SiV3W9O40]-3H(2)O. Accordingly, the toxicity of these two polyoxotungstates with anti-acetylcholinesterase effect cannot be considered as a severe one, but their potential clinical application would require a more complex toxicological study.
T2  - Toxicology and Applied Pharmacology
T1  - Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity
VL  - 333
SP  - 68
EP  - 75
DO  - 10.1016/j.taap.2017.08.010
ER  - 
@article{
author = "Čolović, Mirjana B. and Medic, Branislava and Cetkovic, Mila and Kravić-Stevović, Tamara K. and Stojanovic, Marko and Ayass, Wassim W. and Mougharbel, Ali S. and Radenković, Miroslav and Prostran, Milica and Kortz, Ulrich and Krstić, Danijela Z.",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1748",
abstract = "A toxicity evaluation of two Keggin-type heteropolytungstates, K-7[Ti2PW10O40].6H(2)O and K6H [SiV3W9O40].3H(2)O, with different inhibitory potencies toward acetylcholinesterase activity (IC50 values of 1.04 x 10(-6) and 4.80 x 10(-4) mol/L, respectively) was performed. Wistar albino rats were orally treated with single doses (5 and 50 mg/kg) of both investigated compounds. The biochemical parameters of renal (serum urea and creatinine) and liver function (direct and total bilirubin, alanine transaminase, and aspartate aminotransferase) were determined after 24 h and 14 days. A histopathological analysis of liver tissue was carried out 14 days after the polyoxotungstate administration. Both applied doses of the investigated compounds did not induce statistically significant alterations of the renal function markers. However, the polyoxotungstate treatment caused an increase in the activities of serum alanine transaminase and aspartate aminotransferase in a time- and concentration -dependent manner, although statistically significant changes in bilirubin concentrations were not observed. Furthermore, the detected hepatotoxic effect was confirmed by histhopathological analysis that suggested some reversible liver tissue damage two weeks after the treatment, especially in the case of K6H [SiV3W9O40]-3H(2)O. Accordingly, the toxicity of these two polyoxotungstates with anti-acetylcholinesterase effect cannot be considered as a severe one, but their potential clinical application would require a more complex toxicological study.",
journal = "Toxicology and Applied Pharmacology",
title = "Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity",
volume = "333",
pages = "68-75",
doi = "10.1016/j.taap.2017.08.010"
}
Čolović, M. B., Medic, B., Cetkovic, M., Kravić-Stevović, T. K., Stojanovic, M., Ayass, W. W., Mougharbel, A. S., Radenković, M., Prostran, M., Kortz, U.,& Krstić, D. Z. (2017). Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity.
Toxicology and Applied Pharmacology, 333, 68-75.
https://doi.org/10.1016/j.taap.2017.08.010
Čolović MB, Medic B, Cetkovic M, Kravić-Stevović TK, Stojanovic M, Ayass WW, Mougharbel AS, Radenković M, Prostran M, Kortz U, Krstić DZ. Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity. Toxicology and Applied Pharmacology. 2017;333:68-75
Čolović Mirjana B., Medic Branislava, Cetkovic Mila, Kravić-Stevović Tamara K., Stojanovic Marko, Ayass Wassim W., Mougharbel Ali S., Radenković Miroslav, Prostran Milica, Kortz Ulrich, Krstić Danijela Z., "Toxicity evaluation of two polyoxotungstates with anti-acetylcholinesterase activity" Toxicology and Applied Pharmacology, 333 (2017):68-75,
https://doi.org/10.1016/j.taap.2017.08.010 .
7
8
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9

Na/K-ATPase as a target for anticancer metal based drugs: insights into molecular interactions with selected gold(III) complexes

Bondžić, Aleksandra; Janjić, Goran V.; Dramićanin, Miroslav; Messori, Luigi; Massai, Lara; Parac Vogt, Tatjana N.; Vasić, Vesna M.

(2017)

TY  - JOUR
AU  - Bondžić, Aleksandra
AU  - Janjić, Goran V.
AU  - Dramićanin, Miroslav
AU  - Messori, Luigi
AU  - Massai, Lara
AU  - Parac Vogt, Tatjana N.
AU  - Vasić, Vesna M.
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1476
AB  - Na/K-ATPase is emerging as an important target for a variety of anticancer metal-based drugs. The interactions of Na/K-ATPase (in its E1 state) with three representative and structurally related cytotoxic gold(III) complexes, i.e. [Au(bipy)(OH)(2)][PF6], bipy = 2,2-bipyridine; [Au(py(dmb)-H)(CH3COO)(2)], py(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-pyridine and [Au(bipy(dmb)-H)(OH)][PF6], bipy(c)-H = deprotonated 6-(1,1-dimethylbenzyl)-2,20-bipyridine, are investigated here in depth using a variety of spectroscopic methods, in combination with docking studies. Detailed information is gained on the conformational and structural changes experienced by the enzyme upon binding of these gold(III) complexes. The quenching constants of intrinsic enzyme fluorescence, the fraction of Trp residues accessible to gold(III) complexes and the reaction stoichiometries were determined in various cases. Specific hypotheses are made concerning the binding mode of these gold(III) complexes to the enzyme and the likely binding sites. Differences in their binding behaviour toward Na/K-ATPase are explained on the ground of their distinctive structural features. The present results offer further support to the view that Na/K-ATPase may be a relevant biomolecular target for cytotoxic gold(III) compounds of medicinal interest and may thus be involved in their overall mode of action.
T2  - Metallomics
T1  - Na/K-ATPase as a target for anticancer metal based drugs: insights into molecular interactions with selected gold(III) complexes
VL  - 9
IS  - 3
SP  - 292
EP  - 300
DO  - 10.1039/c7mt00017k
ER  - 
@article{
author = "Bondžić, Aleksandra and Janjić, Goran V. and Dramićanin, Miroslav and Messori, Luigi and Massai, Lara and Parac Vogt, Tatjana N. and Vasić, Vesna M.",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1476",
abstract = "Na/K-ATPase is emerging as an important target for a variety of anticancer metal-based drugs. The interactions of Na/K-ATPase (in its E1 state) with three representative and structurally related cytotoxic gold(III) complexes, i.e. [Au(bipy)(OH)(2)][PF6], bipy = 2,2-bipyridine; [Au(py(dmb)-H)(CH3COO)(2)], py(dmb)-H = deprotonated 6-(1,1-dimethylbenzyl)-pyridine and [Au(bipy(dmb)-H)(OH)][PF6], bipy(c)-H = deprotonated 6-(1,1-dimethylbenzyl)-2,20-bipyridine, are investigated here in depth using a variety of spectroscopic methods, in combination with docking studies. Detailed information is gained on the conformational and structural changes experienced by the enzyme upon binding of these gold(III) complexes. The quenching constants of intrinsic enzyme fluorescence, the fraction of Trp residues accessible to gold(III) complexes and the reaction stoichiometries were determined in various cases. Specific hypotheses are made concerning the binding mode of these gold(III) complexes to the enzyme and the likely binding sites. Differences in their binding behaviour toward Na/K-ATPase are explained on the ground of their distinctive structural features. The present results offer further support to the view that Na/K-ATPase may be a relevant biomolecular target for cytotoxic gold(III) compounds of medicinal interest and may thus be involved in their overall mode of action.",
journal = "Metallomics",
title = "Na/K-ATPase as a target for anticancer metal based drugs: insights into molecular interactions with selected gold(III) complexes",
volume = "9",
number = "3",
pages = "292-300",
doi = "10.1039/c7mt00017k"
}
Bondžić, A., Janjić, G. V., Dramićanin, M., Messori, L., Massai, L., Parac Vogt, T. N.,& Vasić, V. M. (2017). Na/K-ATPase as a target for anticancer metal based drugs: insights into molecular interactions with selected gold(III) complexes.
Metallomics, 9(3), 292-300.
https://doi.org/10.1039/c7mt00017k
Bondžić A, Janjić GV, Dramićanin M, Messori L, Massai L, Parac Vogt TN, Vasić VM. Na/K-ATPase as a target for anticancer metal based drugs: insights into molecular interactions with selected gold(III) complexes. Metallomics. 2017;9(3):292-300
Bondžić Aleksandra, Janjić Goran V., Dramićanin Miroslav, Messori Luigi, Massai Lara, Parac Vogt Tatjana N., Vasić Vesna M., "Na/K-ATPase as a target for anticancer metal based drugs: insights into molecular interactions with selected gold(III) complexes" Metallomics, 9, no. 3 (2017):292-300,
https://doi.org/10.1039/c7mt00017k .
10
10
10

The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro

Petrović, Sandra; Vasić, Vesna M.; Mitrovic, Tatjana; Lazovic, Sasa; Leskovac, Andreja

(2017)

TY  - JOUR
AU  - Petrović, Sandra
AU  - Vasić, Vesna M.
AU  - Mitrovic, Tatjana
AU  - Lazovic, Sasa
AU  - Leskovac, Andreja
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1506
AB  - Undecylprodigiosin pigment (UPP) is reported to display cytotoxic activity towards various types of tumours. Nevertheless, its efficacy in modifying the cellular response to ionising radiation is still unknown. In this study, the radiomodulating effects of UPP were investigated. The effects of UPP were assessed in vitro by treating cultures of human peripheral blood with UPP and ionising radiation using two treatment regimens, the UPP pre-irradiation treatment and UPP post-irradiation treatment. The activity of UPP was investigated evaluating its effects on the radiation-induced micronuclei formation, cell proliferation, and induction of apoptosis. The redox modulating effects of UPP were examined measuring the catalase activity and the level of malondialdehyde, as a measure of oxidative stress. The results showed that UPP effects on cellular response to ionising radiation depend on its concentration and the timing of its administration. At low concentration, the UPP displayed radioprotective effects in. gamma-irradiated human lymphocytes while at higher concentrations, it acted as a radiosensitiser enhancing either mitotic catastrophe or apoptosis depending on the treatment regimen. The UPP modified redox processes in cells, particularly when it was employed prior to gamma-irradiation. Our data highlight the importance of further research of the potential of UPP to sensitize tumour cells to radiation therapy by inhibiting pathways that lead to treatment resistance.
T2  - Arhiv za higijenu rada i toksikologiju
T1  - The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro
VL  - 68
IS  - 1
SP  - 1
EP  - 8
DO  - 10.1515/aiht-2017-68-2897
ER  - 
@article{
author = "Petrović, Sandra and Vasić, Vesna M. and Mitrovic, Tatjana and Lazovic, Sasa and Leskovac, Andreja",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1506",
abstract = "Undecylprodigiosin pigment (UPP) is reported to display cytotoxic activity towards various types of tumours. Nevertheless, its efficacy in modifying the cellular response to ionising radiation is still unknown. In this study, the radiomodulating effects of UPP were investigated. The effects of UPP were assessed in vitro by treating cultures of human peripheral blood with UPP and ionising radiation using two treatment regimens, the UPP pre-irradiation treatment and UPP post-irradiation treatment. The activity of UPP was investigated evaluating its effects on the radiation-induced micronuclei formation, cell proliferation, and induction of apoptosis. The redox modulating effects of UPP were examined measuring the catalase activity and the level of malondialdehyde, as a measure of oxidative stress. The results showed that UPP effects on cellular response to ionising radiation depend on its concentration and the timing of its administration. At low concentration, the UPP displayed radioprotective effects in. gamma-irradiated human lymphocytes while at higher concentrations, it acted as a radiosensitiser enhancing either mitotic catastrophe or apoptosis depending on the treatment regimen. The UPP modified redox processes in cells, particularly when it was employed prior to gamma-irradiation. Our data highlight the importance of further research of the potential of UPP to sensitize tumour cells to radiation therapy by inhibiting pathways that lead to treatment resistance.",
journal = "Arhiv za higijenu rada i toksikologiju",
title = "The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro",
volume = "68",
number = "1",
pages = "1-8",
doi = "10.1515/aiht-2017-68-2897"
}
Petrović, S., Vasić, V. M., Mitrovic, T., Lazovic, S.,& Leskovac, A. (2017). The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro.
Arhiv za higijenu rada i toksikologiju, 68(1), 1-8.
https://doi.org/10.1515/aiht-2017-68-2897
Petrović S, Vasić VM, Mitrovic T, Lazovic S, Leskovac A. The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro. Arhiv za higijenu rada i toksikologiju. 2017;68(1):1-8
Petrović Sandra, Vasić Vesna M., Mitrovic Tatjana, Lazovic Sasa, Leskovac Andreja, "The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro" Arhiv za higijenu rada i toksikologiju, 68, no. 1 (2017):1-8,
https://doi.org/10.1515/aiht-2017-68-2897 .
3
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3