TY  - JOUR
AU  - Vučić, Vesna
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Radojčić, Marija
AU  - Ruždijić, Sabera
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3467
AB  - The antiproliferative and cytotoxic potential of the nucleotide analog 8-Cl-cAMP was tested in PC-3 and DU145 metastatic human prostate cancer cells. The drug was examined as the only therapeutic agent and in combination with ionizing irradiation (IR). Highly synergistic effects of IR and 8-Cl-cAMP were observed in both cell lines when examined by the MTT viability and BrdU proliferation assays. The combination of IR and 8-Cl-cAMP at clinically relevant doses exerted substantial growth inhibition. The combination of IR and 8-Cl-cAMP caused a significant disturbance in the distribution of cell cycle phases. Cell cycle arrest in the sub-G0/G1 phase predominated in both cell lines. The most striking observation was a significant increase in apoptotic PC-3 and DU145 cells. The DU145 cells were three times more sensitive to the combined treatment than PC-3 cells. The initial resistance to IR-induced apoptosis in these p53-deficient prostate cancer cell lines was overcome through an alternative proapoptotic pathway induced by 8-Cl-cAMP. Considering the low effective doses of treatments, improved tumor eradication rates and minimal undesirable side effects, the combination of IR and 8-Cl-cAMP could be the therapy of choice in treating prostate cancer.
T2  - Investigational New Drugs
T1  - The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells
VL  - 26
IS  - 4
SP  - 309
EP  - 317
DO  - 10.1007/s10637-007-9101-4
ER  - 

@article{
author = "Vučić, Vesna and Nićiforović, Ana and Adžić, Miroslav and Radojčić, Marija and Ruždijić, Sabera",
year = "2008",
abstract = "The antiproliferative and cytotoxic potential of the nucleotide analog 8-Cl-cAMP was tested in PC-3 and DU145 metastatic human prostate cancer cells. The drug was examined as the only therapeutic agent and in combination with ionizing irradiation (IR). Highly synergistic effects of IR and 8-Cl-cAMP were observed in both cell lines when examined by the MTT viability and BrdU proliferation assays. The combination of IR and 8-Cl-cAMP at clinically relevant doses exerted substantial growth inhibition. The combination of IR and 8-Cl-cAMP caused a significant disturbance in the distribution of cell cycle phases. Cell cycle arrest in the sub-G0/G1 phase predominated in both cell lines. The most striking observation was a significant increase in apoptotic PC-3 and DU145 cells. The DU145 cells were three times more sensitive to the combined treatment than PC-3 cells. The initial resistance to IR-induced apoptosis in these p53-deficient prostate cancer cell lines was overcome through an alternative proapoptotic pathway induced by 8-Cl-cAMP. Considering the low effective doses of treatments, improved tumor eradication rates and minimal undesirable side effects, the combination of IR and 8-Cl-cAMP could be the therapy of choice in treating prostate cancer.",
journal = "Investigational New Drugs",
title = "The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells",
volume = "26",
number = "4",
pages = "309-317",
doi = "10.1007/s10637-007-9101-4"
}

Vučić, V., Nićiforović, A., Adžić, M., Radojčić, M.,& Ruždijić, S.. (2008). The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells. in Investigational New Drugs, 26(4), 309-317.
https://doi.org/10.1007/s10637-007-9101-4

Vučić V, Nićiforović A, Adžić M, Radojčić M, Ruždijić S. The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells. in Investigational New Drugs. 2008;26(4):309-317.
doi:10.1007/s10637-007-9101-4 .

Vučić, Vesna, Nićiforović, Ana, Adžić, Miroslav, Radojčić, Marija, Ruždijić, Sabera, "The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells" in Investigational New Drugs, 26, no. 4 (2008):309-317,
https://doi.org/10.1007/s10637-007-9101-4 . .