Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications

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Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications (en)
Улога стероидних хормона у неуроендокриној адаптацији на стрес и патофизиологији метаболичког синдрома - молекуларни механизми и клиничке импликације (sr)
Uloga steroidnih hormona u neuroendokrinoj adaptaciji na stres i patofiziologiji metaboličkog sindroma - molekularni mehanizmi i kliničke implikacije (sr_RS)
Authors

Publications

Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats

Romić, Snježana Đ.; Đorđević, Ana; Tepavčević, Snežana; Ćulafić, Tijana; Stojiljković, Mojca D.; Bursać, Biljana; Stanišić, Jelena; Kostić, Milan; Gligorovska, Ljupka; Korićanac, Goran

(2020)

TY  - JOUR
AU  - Romić, Snježana Đ.
AU  - Đorđević, Ana
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca D.
AU  - Bursać, Biljana
AU  - Stanišić, Jelena
AU  - Kostić, Milan
AU  - Gligorovska, Ljupka
AU  - Korićanac, Goran
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8849
AB  - Both a diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine the effects of the fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether these effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress for 9 weeks. Protein expression and/or phosphorylation of the insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by western blot. GR distribution between cytosolic and nuclear fractions was also analyzed. The fructose-rich diet decreased the level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased the IRS1 content and prevented the fructose diet-induced decrease of the pAkt (Ser473) level. The fructose-rich diet in combination with chronic stress reduced the protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased the protein content of Na+/K+-ATPase α1- and α2-subunits, while after combined treatment the α2 content was at the control level and the α1 content was lower than the control level. The effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights into the effects of chronic stress and a combination of the fructose diet and chronic stress on the studied molecules in the heart.
T2  - Food & Function
T1  - Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats
VL  - 11
IS  - 2
SP  - 1455
EP  - 1466
DO  - 10.1039/C9FO02306B
ER  - 
@article{
author = "Romić, Snježana Đ. and Đorđević, Ana and Tepavčević, Snežana and Ćulafić, Tijana and Stojiljković, Mojca D. and Bursać, Biljana and Stanišić, Jelena and Kostić, Milan and Gligorovska, Ljupka and Korićanac, Goran",
year = "2020",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/8849",
abstract = "Both a diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine the effects of the fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether these effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress for 9 weeks. Protein expression and/or phosphorylation of the insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by western blot. GR distribution between cytosolic and nuclear fractions was also analyzed. The fructose-rich diet decreased the level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased the IRS1 content and prevented the fructose diet-induced decrease of the pAkt (Ser473) level. The fructose-rich diet in combination with chronic stress reduced the protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased the protein content of Na+/K+-ATPase α1- and α2-subunits, while after combined treatment the α2 content was at the control level and the α1 content was lower than the control level. The effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights into the effects of chronic stress and a combination of the fructose diet and chronic stress on the studied molecules in the heart.",
journal = "Food & Function",
title = "Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats",
volume = "11",
number = "2",
pages = "1455-1466",
doi = "10.1039/C9FO02306B"
}
Romić, S. Đ., Đorđević, A., Tepavčević, S., Ćulafić, T., Stojiljković, M. D., Bursać, B., Stanišić, J., Kostić, M., Gligorovska, L.,& Korićanac, G. (2020). Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats.
Food & Function, 11(2), 1455-1466.
https://doi.org/10.1039/C9FO02306B
Romić SĐ, Đorđević A, Tepavčević S, Ćulafić T, Stojiljković MD, Bursać B, Stanišić J, Kostić M, Gligorovska L, Korićanac G. Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats. Food & Function. 2020;11(2):1455-1466
Romić Snježana Đ., Đorđević Ana, Tepavčević Snežana, Ćulafić Tijana, Stojiljković Mojca D., Bursać Biljana, Stanišić Jelena, Kostić Milan, Gligorovska Ljupka, Korićanac Goran, "Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats" Food & Function, 11, no. 2 (2020):1455-1466,
https://doi.org/10.1039/C9FO02306B .
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Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle

Đokić, Vladimir; Janković, Svetlana; Labudović-Borović, Milica; Rakočević, Jelena; Stanišić, Jelena; Rajković, Jovana; Novaković, Radmila; Kostić, Milan; Đurić, Miloš; Gostimirović, Miloš; Gojković-Bukarica, Ljiljana

(2020)

TY  - JOUR
AU  - Đokić, Vladimir
AU  - Janković, Svetlana
AU  - Labudović-Borović, Milica
AU  - Rakočević, Jelena
AU  - Stanišić, Jelena
AU  - Rajković, Jovana
AU  - Novaković, Radmila
AU  - Kostić, Milan
AU  - Đurić, Miloš
AU  - Gostimirović, Miloš
AU  - Gojković-Bukarica, Ljiljana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9048
AB  - Voltage-gated potassium (K ) channels are the largest superfamily of potassium (K) channels. A variety of K channels are expressed in the vascular smooth muscle cells (SMC). Studies have shown that gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) cause various changes in the human umbilical vein (HUV). Recently, we have shown that 4-AP, a nonspecific K 1-4 channel inhibitor, significantly decreases vasorelaxation induced by K channel opener pinacidil in vascular SMCs of the HUVs from normal pregnancies, but not in GDM and PIH. The goal of this study was to provide more detailed insight in the K channel subtypes involved in pinacidil-induced vasodilation of HUVs, as well as to investigate potential alterations of their function and expression during GDM and PIH. Margatoxin, a specific blocker of K 1.2 and K 1.3 channels, significantly antagonized pinacidil-induced vasorelaxation in normal pregnancy, while in HUVs from GDM and PIH that was not the case, indicating damage of K 1.2 and K 1.3 channel function. Immunohistochemistry and Western blot revealed similar expression of K 1.2 channels in all groups. The expression of K 1.3 subunit was significantly decreased in PIH, while it remained unchanged in GDM compared to normal pregnancy. Phrixotoxin, specific blocker of K 4.2 and K 4.3 channels, did not antagonize response to pinacidil in any of the groups. The major novel findings show that margatoxin antagonized pinacidil-induced relaxation in normal pregnancy, but not in GDM and PIH. Decreased expression of K 1.3 channels in HUV during PIH may be important pathophysiological mechanism contributing to an increased risk of adverse pregnancy outcomes.
T2  - European Journal of Pharmacology
T1  - Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle
VL  - 882
SP  - 173281
DO  - 10.1016/j.ejphar.2020.173281
ER  - 
@article{
author = "Đokić, Vladimir and Janković, Svetlana and Labudović-Borović, Milica and Rakočević, Jelena and Stanišić, Jelena and Rajković, Jovana and Novaković, Radmila and Kostić, Milan and Đurić, Miloš and Gostimirović, Miloš and Gojković-Bukarica, Ljiljana",
year = "2020",
url = "https://vinar.vin.bg.ac.rs/handle/123456789/9048",
abstract = "Voltage-gated potassium (K ) channels are the largest superfamily of potassium (K) channels. A variety of K channels are expressed in the vascular smooth muscle cells (SMC). Studies have shown that gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) cause various changes in the human umbilical vein (HUV). Recently, we have shown that 4-AP, a nonspecific K 1-4 channel inhibitor, significantly decreases vasorelaxation induced by K channel opener pinacidil in vascular SMCs of the HUVs from normal pregnancies, but not in GDM and PIH. The goal of this study was to provide more detailed insight in the K channel subtypes involved in pinacidil-induced vasodilation of HUVs, as well as to investigate potential alterations of their function and expression during GDM and PIH. Margatoxin, a specific blocker of K 1.2 and K 1.3 channels, significantly antagonized pinacidil-induced vasorelaxation in normal pregnancy, while in HUVs from GDM and PIH that was not the case, indicating damage of K 1.2 and K 1.3 channel function. Immunohistochemistry and Western blot revealed similar expression of K 1.2 channels in all groups. The expression of K 1.3 subunit was significantly decreased in PIH, while it remained unchanged in GDM compared to normal pregnancy. Phrixotoxin, specific blocker of K 4.2 and K 4.3 channels, did not antagonize response to pinacidil in any of the groups. The major novel findings show that margatoxin antagonized pinacidil-induced relaxation in normal pregnancy, but not in GDM and PIH. Decreased expression of K 1.3 channels in HUV during PIH may be important pathophysiological mechanism contributing to an increased risk of adverse pregnancy outcomes.",
journal = "European Journal of Pharmacology",
title = "Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle",
volume = "882",
pages = "173281",
doi = "10.1016/j.ejphar.2020.173281"
}
Đokić, V., Janković, S., Labudović-Borović, M., Rakočević, J., Stanišić, J., Rajković, J., Novaković, R., Kostić, M., Đurić, M., Gostimirović, M.,& Gojković-Bukarica, L. (2020). Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle.
European Journal of Pharmacology, 882, 173281.
https://doi.org/10.1016/j.ejphar.2020.173281
Đokić V, Janković S, Labudović-Borović M, Rakočević J, Stanišić J, Rajković J, Novaković R, Kostić M, Đurić M, Gostimirović M, Gojković-Bukarica L. Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle. European Journal of Pharmacology. 2020;882:173281
Đokić Vladimir, Janković Svetlana, Labudović-Borović Milica, Rakočević Jelena, Stanišić Jelena, Rajković Jovana, Novaković Radmila, Kostić Milan, Đurić Miloš, Gostimirović Miloš, Gojković-Bukarica Ljiljana, "Pregnancy-induced hypertension decreases Kv1.3 potassium channel expression and function in human umbilical vein smooth muscle" European Journal of Pharmacology, 882 (2020):173281,
https://doi.org/10.1016/j.ejphar.2020.173281 .
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Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels

Đokić, Vladimir; Janković-Ražnatović, Svetlana; Novaković, Radmila; Kostić, Milan; Rajković, Jovana; Labudović-Borović, Milica; Rakočević, Jelena T.; Stanišić, Jelena; Đurić, Miloš; Gojković-Bukarica, Ljiljana

(2019)

TY  - JOUR
AU  - Đokić, Vladimir
AU  - Janković-Ražnatović, Svetlana
AU  - Novaković, Radmila
AU  - Kostić, Milan
AU  - Rajković, Jovana
AU  - Labudović-Borović, Milica
AU  - Rakočević, Jelena T.
AU  - Stanišić, Jelena
AU  - Đurić, Miloš
AU  - Gojković-Bukarica, Ljiljana
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8626
AB  - Gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) can jeopardize mother and/or fetus. Vascular ATP-sensitive potassium (KATP) channels most likely participate in the processes of diabetes and hypertension. The aim of this research was to examine whether GDM and PIH cause changes in the expression and function of KATP channels in vascular smooth muscle of human umbilical vein (HUV). Western blot and immunohistochemistry detected significantly decreased expression of Kir6.1 subunit of KATP channels in GDM and PIH, while the expression of SUR2B was unchanged. In GDM, a K+ channel opener, pinacidil caused reduced relaxation of the endothelium-denuded HUVs compared to normal pregnancy. However, its effects in HUVs from PIH subjects were similar to normal pregnancy. In all groups KATP channel blocker glibenclamide antagonized the relaxation of HUV induced by pinacidil without change in the maximal relaxations indicating additional KATP channel-independent mechanisms of pinacidil action. Iberiotoxin, a selective antagonist of large-conductance calcium-activated potassium channels, inhibited the relaxant effect of pinacidil in PIH, but not in normal pregnancy and GDM. Experiments performed in K+-rich solution confirmed the existence of K+-independent effects of pinacidil, which also appear to be impaired in GDM and PIH. Thus, the expression of KATP channels is decreased in GDM and PIH. In GDM, vasorelaxant response of HUV to pinacidil is reduced, while in PIH it remains unchanged. It is very likely that KATP channels modulation and more detailed insight in KATP channel-independent actions of pinacidil may be precious in the therapy of pathological pregnancies. © 2019 Elsevier Inc.
T2  - Experimental and Molecular Pathology
T1  - Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels
VL  - 111
SP  - 104323
DO  - 10.1016/j.yexmp.2019.104323
ER  - 
@article{
author = "Đokić, Vladimir and Janković-Ražnatović, Svetlana and Novaković, Radmila and Kostić, Milan and Rajković, Jovana and Labudović-Borović, Milica and Rakočević, Jelena T. and Stanišić, Jelena and Đurić, Miloš and Gojković-Bukarica, Ljiljana",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8626",
abstract = "Gestational diabetes mellitus (GDM) and pregnancy-induced hypertension (PIH) can jeopardize mother and/or fetus. Vascular ATP-sensitive potassium (KATP) channels most likely participate in the processes of diabetes and hypertension. The aim of this research was to examine whether GDM and PIH cause changes in the expression and function of KATP channels in vascular smooth muscle of human umbilical vein (HUV). Western blot and immunohistochemistry detected significantly decreased expression of Kir6.1 subunit of KATP channels in GDM and PIH, while the expression of SUR2B was unchanged. In GDM, a K+ channel opener, pinacidil caused reduced relaxation of the endothelium-denuded HUVs compared to normal pregnancy. However, its effects in HUVs from PIH subjects were similar to normal pregnancy. In all groups KATP channel blocker glibenclamide antagonized the relaxation of HUV induced by pinacidil without change in the maximal relaxations indicating additional KATP channel-independent mechanisms of pinacidil action. Iberiotoxin, a selective antagonist of large-conductance calcium-activated potassium channels, inhibited the relaxant effect of pinacidil in PIH, but not in normal pregnancy and GDM. Experiments performed in K+-rich solution confirmed the existence of K+-independent effects of pinacidil, which also appear to be impaired in GDM and PIH. Thus, the expression of KATP channels is decreased in GDM and PIH. In GDM, vasorelaxant response of HUV to pinacidil is reduced, while in PIH it remains unchanged. It is very likely that KATP channels modulation and more detailed insight in KATP channel-independent actions of pinacidil may be precious in the therapy of pathological pregnancies. © 2019 Elsevier Inc.",
journal = "Experimental and Molecular Pathology",
title = "Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels",
volume = "111",
pages = "104323",
doi = "10.1016/j.yexmp.2019.104323"
}
Đokić, V., Janković-Ražnatović, S., Novaković, R., Kostić, M., Rajković, J., Labudović-Borović, M., Rakočević, J. T., Stanišić, J., Đurić, M.,& Gojković-Bukarica, L. (2019). Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels.
Experimental and Molecular Pathology, 111, 104323.
https://doi.org/10.1016/j.yexmp.2019.104323
Đokić V, Janković-Ražnatović S, Novaković R, Kostić M, Rajković J, Labudović-Borović M, Rakočević JT, Stanišić J, Đurić M, Gojković-Bukarica L. Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels. Experimental and Molecular Pathology. 2019;111:104323
Đokić Vladimir, Janković-Ražnatović Svetlana, Novaković Radmila, Kostić Milan, Rajković Jovana, Labudović-Borović Milica, Rakočević Jelena T., Stanišić Jelena, Đurić Miloš, Gojković-Bukarica Ljiljana, "Effect of gestational diabetes mellitus and pregnancy-induced hypertension on human umbilical vein smooth muscle KATP channels" Experimental and Molecular Pathology, 111 (2019):104323,
https://doi.org/10.1016/j.yexmp.2019.104323 .
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Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats

Bošković, Maja; Bundalo, Maja M.; Živković, Maja; Stanišić, Jelena; Kostić, Milan; Korićanac, Goran; Stanković, Aleksandra

(2019)

TY  - JOUR
AU  - Bošković, Maja
AU  - Bundalo, Maja M.
AU  - Živković, Maja
AU  - Stanišić, Jelena
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7984
AB  - Harmful effects of fructose-rich diet (FRD) were predominantly observed in males, suggesting protective effects of estrogens. Little is known about AMPK/sirtuin-1 (SIRT1)/forkhead box O3 (FoxO3a)/manganese superoxide dismutase (MnSOD)/catalase signaling in the heart in state of metabolic syndrome and oxidative stress induced by fructose over-consumption. We investigated the effect of 10% FRD on expression of AMPK-SIRT1-FoxO3a-MnSOD/catalase axis in myocardium and potentially beneficial effect of 17β-estradiol replacement. The expression of NADPH oxidase 4 (Nox4) and miRNA-155, unfavorable regulators of this axis, were also investigated. FRD significantly increased AMPK and decreased FoxO3a activity, decreased SIRT1, MnSOD and Nox4 protein expression while E2 reverted these changes, except for Nox4, and increased catalase protein level. E2 diminished Nox4 and MnSOD mRNA level in FRD ovariectomized rats. These results suggest independent response of AMPK and SIRT to FRD treatment. The proposed signaling in the heart should be further investigated in the prooxidative and antioxidative milieu.
T2  - Journal of Functional Foods
T1  - Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats
VL  - 52
SP  - 690
EP  - 698
DO  - 10.1016/j.jff.2018.11.053
ER  - 
@article{
author = "Bošković, Maja and Bundalo, Maja M. and Živković, Maja and Stanišić, Jelena and Kostić, Milan and Korićanac, Goran and Stanković, Aleksandra",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/7984",
abstract = "Harmful effects of fructose-rich diet (FRD) were predominantly observed in males, suggesting protective effects of estrogens. Little is known about AMPK/sirtuin-1 (SIRT1)/forkhead box O3 (FoxO3a)/manganese superoxide dismutase (MnSOD)/catalase signaling in the heart in state of metabolic syndrome and oxidative stress induced by fructose over-consumption. We investigated the effect of 10% FRD on expression of AMPK-SIRT1-FoxO3a-MnSOD/catalase axis in myocardium and potentially beneficial effect of 17β-estradiol replacement. The expression of NADPH oxidase 4 (Nox4) and miRNA-155, unfavorable regulators of this axis, were also investigated. FRD significantly increased AMPK and decreased FoxO3a activity, decreased SIRT1, MnSOD and Nox4 protein expression while E2 reverted these changes, except for Nox4, and increased catalase protein level. E2 diminished Nox4 and MnSOD mRNA level in FRD ovariectomized rats. These results suggest independent response of AMPK and SIRT to FRD treatment. The proposed signaling in the heart should be further investigated in the prooxidative and antioxidative milieu.",
journal = "Journal of Functional Foods",
title = "Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats",
volume = "52",
pages = "690-698",
doi = "10.1016/j.jff.2018.11.053"
}
Bošković, M., Bundalo, M. M., Živković, M., Stanišić, J., Kostić, M., Korićanac, G.,& Stanković, A. (2019). Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats.
Journal of Functional Foods, 52, 690-698.
https://doi.org/10.1016/j.jff.2018.11.053
Bošković M, Bundalo MM, Živković M, Stanišić J, Kostić M, Korićanac G, Stanković A. Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats. Journal of Functional Foods. 2019;52:690-698
Bošković Maja, Bundalo Maja M., Živković Maja, Stanišić Jelena, Kostić Milan, Korićanac Goran, Stanković Aleksandra, "Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats" Journal of Functional Foods, 52 (2019):690-698,
https://doi.org/10.1016/j.jff.2018.11.053 .
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The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows

Pantelić, Marija; Jovanović, Ljubomir J.; Prodanović, Radiša; Vujanac, Ivan; Đurić, Miloje; Ćulafić, Tijana; Vranješ-Đurić, Sanja; Korićanac, Goran; Kirovski, Danijela

(2018)

TY  - JOUR
AU  - Pantelić, Marija
AU  - Jovanović, Ljubomir J.
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Ćulafić, Tijana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jpn.12655
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7804
AB  - Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods.
T2  - Journal of Animal Physiology and Animal Nutrition
T1  - The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows
VL  - 102
IS  - 1
SP  - 41
EP  - 55
DO  - 10.1111/jpn.12655
ER  - 
@article{
author = "Pantelić, Marija and Jovanović, Ljubomir J. and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Ćulafić, Tijana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2018",
url = "http://doi.wiley.com/10.1111/jpn.12655, http://vinar.vin.bg.ac.rs/handle/123456789/7804",
abstract = "Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods.",
journal = "Journal of Animal Physiology and Animal Nutrition",
title = "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows",
volume = "102",
number = "1",
pages = "41-55",
doi = "10.1111/jpn.12655"
}
Pantelić, M., Jovanović, L. J., Prodanović, R., Vujanac, I., Đurić, M., Ćulafić, T., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D. (2018). The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows.
Journal of Animal Physiology and Animal Nutrition, 102(1), 41-55.
https://doi.org/10.1111/jpn.12655
Pantelić M, Jovanović LJ, Prodanović R, Vujanac I, Đurić M, Ćulafić T, Vranješ-Đurić S, Korićanac G, Kirovski D. The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. Journal of Animal Physiology and Animal Nutrition. 2018;102(1):41-55
Pantelić Marija, Jovanović Ljubomir J., Prodanović Radiša, Vujanac Ivan, Đurić Miloje, Ćulafić Tijana, Vranješ-Đurić Sanja, Korićanac Goran, Kirovski Danijela, "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows" Journal of Animal Physiology and Animal Nutrition, 102, no. 1 (2018):41-55,
https://doi.org/10.1111/jpn.12655 .
9
6
8

The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows

Pantelić, Marija; Jovanović, Ljubomir J.; Prodanović, Radiša; Vujanac, Ivan; Đurić, Miloje; Ćulafić, Tijana; Vranješ-Đurić, Sanja; Korićanac, Goran; Kirovski, Danijela

(2018)

TY  - JOUR
AU  - Pantelić, Marija
AU  - Jovanović, Ljubomir J.
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Ćulafić, Tijana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jpn.12655
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7835
AB  - Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods. © 2017 Blackwell Verlag GmbH
T2  - Journal of Animal Physiology and Animal Nutrition
T1  - The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows
VL  - 102
IS  - 1
SP  - 41
EP  - 55
DO  - 10.1111/jpn.12655
ER  - 
@article{
author = "Pantelić, Marija and Jovanović, Ljubomir J. and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Ćulafić, Tijana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2018",
url = "http://doi.wiley.com/10.1111/jpn.12655, http://vinar.vin.bg.ac.rs/handle/123456789/7835",
abstract = "Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods. © 2017 Blackwell Verlag GmbH",
journal = "Journal of Animal Physiology and Animal Nutrition",
title = "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows",
volume = "102",
number = "1",
pages = "41-55",
doi = "10.1111/jpn.12655"
}
Pantelić, M., Jovanović, L. J., Prodanović, R., Vujanac, I., Đurić, M., Ćulafić, T., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D. (2018). The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows.
Journal of Animal Physiology and Animal Nutrition, 102(1), 41-55.
https://doi.org/10.1111/jpn.12655
Pantelić M, Jovanović LJ, Prodanović R, Vujanac I, Đurić M, Ćulafić T, Vranješ-Đurić S, Korićanac G, Kirovski D. The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. Journal of Animal Physiology and Animal Nutrition. 2018;102(1):41-55
Pantelić Marija, Jovanović Ljubomir J., Prodanović Radiša, Vujanac Ivan, Đurić Miloje, Ćulafić Tijana, Vranješ-Đurić Sanja, Korićanac Goran, Kirovski Danijela, "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows" Journal of Animal Physiology and Animal Nutrition, 102, no. 1 (2018):41-55,
https://doi.org/10.1111/jpn.12655 .
9
6
8

Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves

Jovanović, Ljubomir J.; Pantelić, Marija; Prodanović, Radiša; Vujanac, Ivan; Đurić, Miloje; Tepavčević, Snežana; Vranješ-Đurić, Sanja; Korićanac, Goran; Kirovski, Danijela

(2017)

TY  - JOUR
AU  - Jovanović, Ljubomir J.
AU  - Pantelić, Marija
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Tepavčević, Snežana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1808
AB  - The objective of this study was to investigate the effects of peroral administration of chromium-enriched yeast on glucose tolerance in Holstein calves, assessed by insulin signaling pathway molecule determination and intravenous glucose tolerance test (IVGTT). Twenty-four Holstein calves, aged 1 month, were chosen for the study and divided into two groups: the PoCr group (n = 12) that perorally received 0.04 mg of Cr/kg of body mass daily, for 70 days, and the NCr group (n = 12) that received no chromium supplementation. Skeletal tissue samples from each calf were obtained on day 0 and day 70 of the experiment. Chromium supplementation increased protein content of the insulin beta-subunit receptor, phosphorylation of insulin receptor substrate 1 at Tyrosine 632, phosphorylation of Akt at Serine 473, glucose transporter-4, and AMP-activated protein kinase in skeletal muscle tissue, while phosphorylation of insulin receptor substrate 1 at Serine 307 was not affected by chromium treatment. Results obtained during IVGTT, which was conducted on days 0, 30, 50, and 70, suggested an increased insulin sensitivity and, consequently, a better utilization of glucose in the PoCr group. Lower basal concentrations of glucose and insulin in the PoCr group on days 30 and 70 were also obtained. Our results indicate that chromium supplementation improves glucose utilization in calves by enhancing insulin intracellular signaling in the skeletal muscle tissue.
T2  - Biological Trace Element Research
T1  - Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves
VL  - 180
IS  - 2
SP  - 223
EP  - 232
DO  - 10.1007/s12011-017-1007-1
ER  - 
@article{
author = "Jovanović, Ljubomir J. and Pantelić, Marija and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Tepavčević, Snežana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1808",
abstract = "The objective of this study was to investigate the effects of peroral administration of chromium-enriched yeast on glucose tolerance in Holstein calves, assessed by insulin signaling pathway molecule determination and intravenous glucose tolerance test (IVGTT). Twenty-four Holstein calves, aged 1 month, were chosen for the study and divided into two groups: the PoCr group (n = 12) that perorally received 0.04 mg of Cr/kg of body mass daily, for 70 days, and the NCr group (n = 12) that received no chromium supplementation. Skeletal tissue samples from each calf were obtained on day 0 and day 70 of the experiment. Chromium supplementation increased protein content of the insulin beta-subunit receptor, phosphorylation of insulin receptor substrate 1 at Tyrosine 632, phosphorylation of Akt at Serine 473, glucose transporter-4, and AMP-activated protein kinase in skeletal muscle tissue, while phosphorylation of insulin receptor substrate 1 at Serine 307 was not affected by chromium treatment. Results obtained during IVGTT, which was conducted on days 0, 30, 50, and 70, suggested an increased insulin sensitivity and, consequently, a better utilization of glucose in the PoCr group. Lower basal concentrations of glucose and insulin in the PoCr group on days 30 and 70 were also obtained. Our results indicate that chromium supplementation improves glucose utilization in calves by enhancing insulin intracellular signaling in the skeletal muscle tissue.",
journal = "Biological Trace Element Research",
title = "Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves",
volume = "180",
number = "2",
pages = "223-232",
doi = "10.1007/s12011-017-1007-1"
}
Jovanović, L. J., Pantelić, M., Prodanović, R., Vujanac, I., Đurić, M., Tepavčević, S., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D. (2017). Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves.
Biological Trace Element Research, 180(2), 223-232.
https://doi.org/10.1007/s12011-017-1007-1
Jovanović LJ, Pantelić M, Prodanović R, Vujanac I, Đurić M, Tepavčević S, Vranješ-Đurić S, Korićanac G, Kirovski D. Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves. Biological Trace Element Research. 2017;180(2):223-232
Jovanović Ljubomir J., Pantelić Marija, Prodanović Radiša, Vujanac Ivan, Đurić Miloje, Tepavčević Snežana, Vranješ-Đurić Sanja, Korićanac Goran, Kirovski Danijela, "Effect of Peroral Administration of Chromium on Insulin Signaling Pathway in Skeletal Muscle Tissue of Holstein Calves" Biological Trace Element Research, 180, no. 2 (2017):223-232,
https://doi.org/10.1007/s12011-017-1007-1 .
1
8
7
7

Fructose-rich diet differently affects angiotensin II receptor content in the nucleus and a plasma membrane fraction of visceral adipose tissue

Bundalo, Maja M.; Đorđević, Ana D.; Bursac, Biljana; Živković, Maja; Korićanac, Goran; Stanković, Aleksandra

(2017)

TY  - JOUR
AU  - Bundalo, Maja M.
AU  - Đorđević, Ana D.
AU  - Bursac, Biljana
AU  - Živković, Maja
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1839
AB  - The adipose tissue renin-angiotensin system (RAS) is proposed to be a pathophysiological link between adipose tissue dysregulation and metabolic disorders induced by a fructose-rich diet (FRD). RAS can act intracellularly. We hypothesized that adipocyte nuclear membranes possess angiotensin receptor types 1 and 2 (AT1R and AT2R), which couple to nuclear signaling pathways and regulate oxidative gene expression under FRD conditions. We analyzed the effect of consumption of 10% fructose solution for 9 weeks on biochemical parameters, adipocyte morphology, and expression of AT1R, AT2R, AT1R-associated protein (ATRAP), NADPH oxidase 4 (NOX4), matrix metalloproteinase-9 (MMP-9), and manganese superoxide dismutase (MnSOD) in adipose tissue of Wistar rats. We detected AT1R and AT2R in the nuclear fraction. FRD reduced the level of angiotensin receptors in the nucleus, while increased AT1R and decreased AT2R levels were observed in the plasma membrane. FRD increased the ATRAP mRNA level and decreased MnSOD mRNA and protein levels. No significant differences were observed for MMP-9 and NOX4 mRNA levels. These findings coincided with hyperleptinemia, elevated blood pressure and triglycerides, and unchanged visceral adipose tissue mass and morphology in FRD rats. Besides providing evidence for nuclear localization of angiotensin receptors in visceral adipose tissue, this study demonstrates the different effects of FRD on AT1R expression in different cellular compartments. Elevated blood pressure and decreased antioxidant capacity in visceral fat of fructose-fed rats were accompanied by an increased AT1R level in the plasma membrane, while upregulation of ATRAP and a decrease of nuclear membrane AT1R suggest an increased capacity for attenuation of excessive AT1R signaling and visceral adiposity.
T2  - Applied Physiology Nutrition and Metabolism
T1  - Fructose-rich diet differently affects angiotensin II receptor content in the nucleus and a plasma membrane fraction of visceral adipose tissue
VL  - 42
IS  - 12
SP  - 1254
EP  - 1263
DO  - 10.1139/apnm-2016-0725
ER  - 
@article{
author = "Bundalo, Maja M. and Đorđević, Ana D. and Bursac, Biljana and Živković, Maja and Korićanac, Goran and Stanković, Aleksandra",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1839",
abstract = "The adipose tissue renin-angiotensin system (RAS) is proposed to be a pathophysiological link between adipose tissue dysregulation and metabolic disorders induced by a fructose-rich diet (FRD). RAS can act intracellularly. We hypothesized that adipocyte nuclear membranes possess angiotensin receptor types 1 and 2 (AT1R and AT2R), which couple to nuclear signaling pathways and regulate oxidative gene expression under FRD conditions. We analyzed the effect of consumption of 10% fructose solution for 9 weeks on biochemical parameters, adipocyte morphology, and expression of AT1R, AT2R, AT1R-associated protein (ATRAP), NADPH oxidase 4 (NOX4), matrix metalloproteinase-9 (MMP-9), and manganese superoxide dismutase (MnSOD) in adipose tissue of Wistar rats. We detected AT1R and AT2R in the nuclear fraction. FRD reduced the level of angiotensin receptors in the nucleus, while increased AT1R and decreased AT2R levels were observed in the plasma membrane. FRD increased the ATRAP mRNA level and decreased MnSOD mRNA and protein levels. No significant differences were observed for MMP-9 and NOX4 mRNA levels. These findings coincided with hyperleptinemia, elevated blood pressure and triglycerides, and unchanged visceral adipose tissue mass and morphology in FRD rats. Besides providing evidence for nuclear localization of angiotensin receptors in visceral adipose tissue, this study demonstrates the different effects of FRD on AT1R expression in different cellular compartments. Elevated blood pressure and decreased antioxidant capacity in visceral fat of fructose-fed rats were accompanied by an increased AT1R level in the plasma membrane, while upregulation of ATRAP and a decrease of nuclear membrane AT1R suggest an increased capacity for attenuation of excessive AT1R signaling and visceral adiposity.",
journal = "Applied Physiology Nutrition and Metabolism",
title = "Fructose-rich diet differently affects angiotensin II receptor content in the nucleus and a plasma membrane fraction of visceral adipose tissue",
volume = "42",
number = "12",
pages = "1254-1263",
doi = "10.1139/apnm-2016-0725"
}
Bundalo, M. M., Đorđević, A. D., Bursac, B., Živković, M., Korićanac, G.,& Stanković, A. (2017). Fructose-rich diet differently affects angiotensin II receptor content in the nucleus and a plasma membrane fraction of visceral adipose tissue.
Applied Physiology Nutrition and Metabolism, 42(12), 1254-1263.
https://doi.org/10.1139/apnm-2016-0725
Bundalo MM, Đorđević AD, Bursac B, Živković M, Korićanac G, Stanković A. Fructose-rich diet differently affects angiotensin II receptor content in the nucleus and a plasma membrane fraction of visceral adipose tissue. Applied Physiology Nutrition and Metabolism. 2017;42(12):1254-1263
Bundalo Maja M., Đorđević Ana D., Bursac Biljana, Živković Maja, Korićanac Goran, Stanković Aleksandra, "Fructose-rich diet differently affects angiotensin II receptor content in the nucleus and a plasma membrane fraction of visceral adipose tissue" Applied Physiology Nutrition and Metabolism, 42, no. 12 (2017):1254-1263,
https://doi.org/10.1139/apnm-2016-0725 .
1
3
3
2

Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?

Bundalo, Maja M.; Romić, Snježana Đ.; Tepavčević, Snežana; Stojiljković, Mojca D.; Stanković, Aleksandra; Živković, Maja; Korićanac, Goran

(2017)

TY  - JOUR
AU  - Bundalo, Maja M.
AU  - Romić, Snježana Đ.
AU  - Tepavčević, Snežana
AU  - Stojiljković, Mojca D.
AU  - Stanković, Aleksandra
AU  - Živković, Maja
AU  - Korićanac, Goran
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1726
AB  - Increased intake of fructose in humans and laboratory animals is demonstrated to be a risk factor for development of metabolic disorders (insulin resistance, metabolic syndrome, type 2 diabetes) and cardiovascular diseases. On the other hand, estradiol is emphasized as a cardioprotective agent. The main goal of this review is to summarize recent findings on damaging cardiac effects of fructose-rich diet in females, mostly experimental animals, and to evaluate protective capacity of estradiol. Published results of our and other research groups indicate mostly detrimental effects of fructose-rich diet on cardiac insulin signaling molecules, glucose and fatty acid metabolism, nitric oxide production and ion transport, as well as renin-angiotensin system and inflammation. Some of these processes are involved in cardiac insulin signal transmission, others are regulated by insulin or have an influence on insulin action. Administration of estradiol to ovariectomized female rats, exposed to increased intake of fructose, was mostly beneficial to the heart, but sometimes it was ineffective or even detrimental, depending on the particular processes. We believe that these data, carefully translated to human population, could be useful for clinicians dealing with postmenopausal women susceptible to metabolic diseases and hormone replacement therapy.
T2  - European Journal of Pharmacology
T1  - Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?
VL  - 811
SP  - 141
EP  - 147
DO  - 10.1016/j.ejphar.2017.06.003
ER  - 
@article{
author = "Bundalo, Maja M. and Romić, Snježana Đ. and Tepavčević, Snežana and Stojiljković, Mojca D. and Stanković, Aleksandra and Živković, Maja and Korićanac, Goran",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1726",
abstract = "Increased intake of fructose in humans and laboratory animals is demonstrated to be a risk factor for development of metabolic disorders (insulin resistance, metabolic syndrome, type 2 diabetes) and cardiovascular diseases. On the other hand, estradiol is emphasized as a cardioprotective agent. The main goal of this review is to summarize recent findings on damaging cardiac effects of fructose-rich diet in females, mostly experimental animals, and to evaluate protective capacity of estradiol. Published results of our and other research groups indicate mostly detrimental effects of fructose-rich diet on cardiac insulin signaling molecules, glucose and fatty acid metabolism, nitric oxide production and ion transport, as well as renin-angiotensin system and inflammation. Some of these processes are involved in cardiac insulin signal transmission, others are regulated by insulin or have an influence on insulin action. Administration of estradiol to ovariectomized female rats, exposed to increased intake of fructose, was mostly beneficial to the heart, but sometimes it was ineffective or even detrimental, depending on the particular processes. We believe that these data, carefully translated to human population, could be useful for clinicians dealing with postmenopausal women susceptible to metabolic diseases and hormone replacement therapy.",
journal = "European Journal of Pharmacology",
title = "Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?",
volume = "811",
pages = "141-147",
doi = "10.1016/j.ejphar.2017.06.003"
}
Bundalo, M. M., Romić, S. Đ., Tepavčević, S., Stojiljković, M. D., Stanković, A., Živković, M.,& Korićanac, G. (2017). Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?.
European Journal of Pharmacology, 811, 141-147.
https://doi.org/10.1016/j.ejphar.2017.06.003
Bundalo MM, Romić SĐ, Tepavčević S, Stojiljković MD, Stanković A, Živković M, Korićanac G. Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?. European Journal of Pharmacology. 2017;811:141-147
Bundalo Maja M., Romić Snježana Đ., Tepavčević Snežana, Stojiljković Mojca D., Stanković Aleksandra, Živković Maja, Korićanac Goran, "Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?" European Journal of Pharmacology, 811 (2017):141-147,
https://doi.org/10.1016/j.ejphar.2017.06.003 .
1
4
4
4

Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats

Romić, Snježana Đ.; Krskova, Katarina; Olszanecki, Rafal; Balazova, Lucia; Lory, Viktoria; Korićanac, Goran; Slamkova, Miroslava; Zorad, Stefan

(2017)

TY  - JOUR
AU  - Romić, Snježana Đ.
AU  - Krskova, Katarina
AU  - Olszanecki, Rafal
AU  - Balazova, Lucia
AU  - Lory, Viktoria
AU  - Korićanac, Goran
AU  - Slamkova, Miroslava
AU  - Zorad, Stefan
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1764
AB  - Fatty acid (FA) uptake and/or intramuscular triglyceride (TG) accumulation in skeletal muscle are increased in obesity, type 2 diabetes and aging. FA translocase (FAT/CD36) translocation, lipin-1 subcellular localization and nuclear factor kappa B (NF-kappa B) p65 protein content in quadriceps muscle of young and old obese Zuckerfa/fa rats and their lean controls were analyzed by immunoblot to define obesity- and aging-related alterations in FA uptake, their subsequent metabolic fate and potential to activate pro-inflammatory signaling. As expected, obesity increased FAT/CD36 content in plasma membrane in quadriceps muscle of fa/fa rats. Aging increased cytosolic lipin-1 content in both, obese rats and their lean controls. Also, old obese rats had decreased level of nuclear extract lipin-lcompared to that in old lean rats. Neither obesity nor age altered NF-kappa B p65 protein content in cytosol and nuclear extract of quadriceps muscle suggesting that obesity/aging-induced changes in FA handling are not accompanied by NF-kappa B-mediated inflammation. Increase in plasma membrane FAT/CD36 content in obese rats and failure in lipin-1 export to nucleus with progression of obesity, implying an increase in FA uptake and their different channeling into lipid intermediates synthesis pathway in old fa/fa rats versus FA usage in lean rats of the same age.
T2  - General Physiology and Biophysics
T1  - Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats
VL  - 36
IS  - 4
SP  - 399
EP  - 406
DO  - 10.4149/gpb_2017010
ER  - 
@article{
author = "Romić, Snježana Đ. and Krskova, Katarina and Olszanecki, Rafal and Balazova, Lucia and Lory, Viktoria and Korićanac, Goran and Slamkova, Miroslava and Zorad, Stefan",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1764",
abstract = "Fatty acid (FA) uptake and/or intramuscular triglyceride (TG) accumulation in skeletal muscle are increased in obesity, type 2 diabetes and aging. FA translocase (FAT/CD36) translocation, lipin-1 subcellular localization and nuclear factor kappa B (NF-kappa B) p65 protein content in quadriceps muscle of young and old obese Zuckerfa/fa rats and their lean controls were analyzed by immunoblot to define obesity- and aging-related alterations in FA uptake, their subsequent metabolic fate and potential to activate pro-inflammatory signaling. As expected, obesity increased FAT/CD36 content in plasma membrane in quadriceps muscle of fa/fa rats. Aging increased cytosolic lipin-1 content in both, obese rats and their lean controls. Also, old obese rats had decreased level of nuclear extract lipin-lcompared to that in old lean rats. Neither obesity nor age altered NF-kappa B p65 protein content in cytosol and nuclear extract of quadriceps muscle suggesting that obesity/aging-induced changes in FA handling are not accompanied by NF-kappa B-mediated inflammation. Increase in plasma membrane FAT/CD36 content in obese rats and failure in lipin-1 export to nucleus with progression of obesity, implying an increase in FA uptake and their different channeling into lipid intermediates synthesis pathway in old fa/fa rats versus FA usage in lean rats of the same age.",
journal = "General Physiology and Biophysics",
title = "Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats",
volume = "36",
number = "4",
pages = "399-406",
doi = "10.4149/gpb_2017010"
}
Romić, S. Đ., Krskova, K., Olszanecki, R., Balazova, L., Lory, V., Korićanac, G., Slamkova, M.,& Zorad, S. (2017). Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats.
General Physiology and Biophysics, 36(4), 399-406.
https://doi.org/10.4149/gpb_2017010
Romić SĐ, Krskova K, Olszanecki R, Balazova L, Lory V, Korićanac G, Slamkova M, Zorad S. Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats. General Physiology and Biophysics. 2017;36(4):399-406
Romić Snježana Đ., Krskova Katarina, Olszanecki Rafal, Balazova Lucia, Lory Viktoria, Korićanac Goran, Slamkova Miroslava, Zorad Stefan, "Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats" General Physiology and Biophysics, 36, no. 4 (2017):399-406,
https://doi.org/10.4149/gpb_2017010 .
2
2
2

Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression

Prodanović, Radiša; Korićanac, Goran; Vujanac, Ivan; Đorđević, Ana D.; Pantelić, Marija; Romić, Snježana Đ.; Stanimirovic, Zoran; Kirovski, Danijela

(2016)

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Korićanac, Goran
AU  - Vujanac, Ivan
AU  - Đorđević, Ana D.
AU  - Pantelić, Marija
AU  - Romić, Snježana Đ.
AU  - Stanimirovic, Zoran
AU  - Kirovski, Danijela
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1211
AB  - We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 LT = BCS LT = 3.5) and high (4.0 LT = BCS LT = 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation. (C) 2016 Elsevier Ltd. All rights reserved.
T2  - Research in Veterinary Science
T1  - Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression
VL  - 107
SP  - 16
EP  - 19
DO  - 10.1016/j.rvsc.2016.04.007
ER  - 
@article{
author = "Prodanović, Radiša and Korićanac, Goran and Vujanac, Ivan and Đorđević, Ana D. and Pantelić, Marija and Romić, Snježana Đ. and Stanimirovic, Zoran and Kirovski, Danijela",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1211",
abstract = "We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 LT = BCS LT = 3.5) and high (4.0 LT = BCS LT = 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation. (C) 2016 Elsevier Ltd. All rights reserved.",
journal = "Research in Veterinary Science",
title = "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression",
volume = "107",
pages = "16-19",
doi = "10.1016/j.rvsc.2016.04.007"
}
Prodanović, R., Korićanac, G., Vujanac, I., Đorđević, A. D., Pantelić, M., Romić, S. Đ., Stanimirovic, Z.,& Kirovski, D. (2016). Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression.
Research in Veterinary Science, 107, 16-19.
https://doi.org/10.1016/j.rvsc.2016.04.007
Prodanović R, Korićanac G, Vujanac I, Đorđević AD, Pantelić M, Romić SĐ, Stanimirovic Z, Kirovski D. Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. Research in Veterinary Science. 2016;107:16-19
Prodanović Radiša, Korićanac Goran, Vujanac Ivan, Đorđević Ana D., Pantelić Marija, Romić Snježana Đ., Stanimirovic Zoran, Kirovski Danijela, "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression" Research in Veterinary Science, 107 (2016):16-19,
https://doi.org/10.1016/j.rvsc.2016.04.007 .
15
12
13

Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet

Stanišić, Jelena; Korićanac, Goran; Ćulafić, Tijana; Romić, Snježana Đ.; Stojiljković, Mojca D.; Kostić, Milan; Pantelić, Marija; Tepavčević, Snežana

(2016)

TY  - JOUR
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Pantelić, Marija
AU  - Tepavčević, Snežana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/913
AB  - Increase in fructose consumption together with decrease in physical activity contributes to the development of metabolic syndrome and consequently cardiovascular diseases. The current study examined the preventive role of exercise on defects in cardiac insulin signaling and function of endothelial nitric oxide synthase (eNOS) in fructose fed rats. Male Wistar rats were divided into control, sedentary fructose (received 10% fructose for 9 weeks) and exercise fructose (additionally exposed to low intensity exercise) groups. Concentration of triglycerides, glucose, insulin and visceral adipose tissue weight were determined to estimate metabolic syndrome development. Expression and/or phosphorylation of cardiac insulin receptor (IR), insulin receptor substrate 1 (IRS1), tyrosine-specific protein phosphatase 1B (PTP1B), Akt, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and eNOS were evaluated. Fructose overload increased visceral adipose tissue, insulin concentration and homeostasis model assessment index. Exercise managed to decrease visceral adiposity and insulin level and to increase insulin sensitivity. Fructose diet increased level of cardiac PTP1B and pIRS1 (Ser307), while levels of IR and ERK1/2, as well as pIRS1 (Tyr 632), pAkt (Ser473, Thr308) and pERK1/2 were decreased. These disturbances were accompanied by reduced phosphorylation of eNOS at Ser1177. Exercise managed to prevent most of the disturbances in insulin signaling caused by fructose diet (except phosphorylation of IRS1 at Tyr 632 and phosphorylation and protein expression of ERK1/2) and consequently restored function of eNOS. Low intensity exercise could be considered as efficient treatment of cardiac insulin resistance induced by fructose diet. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
T2  - Molecular and Cellular Endocrinology
T1  - Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet
VL  - 420
IS  - C
SP  - 97
EP  - 104
DO  - 10.1016/j.mce.2015.11.032
ER  - 
@article{
author = "Stanišić, Jelena and Korićanac, Goran and Ćulafić, Tijana and Romić, Snježana Đ. and Stojiljković, Mojca D. and Kostić, Milan and Pantelić, Marija and Tepavčević, Snežana",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/913",
abstract = "Increase in fructose consumption together with decrease in physical activity contributes to the development of metabolic syndrome and consequently cardiovascular diseases. The current study examined the preventive role of exercise on defects in cardiac insulin signaling and function of endothelial nitric oxide synthase (eNOS) in fructose fed rats. Male Wistar rats were divided into control, sedentary fructose (received 10% fructose for 9 weeks) and exercise fructose (additionally exposed to low intensity exercise) groups. Concentration of triglycerides, glucose, insulin and visceral adipose tissue weight were determined to estimate metabolic syndrome development. Expression and/or phosphorylation of cardiac insulin receptor (IR), insulin receptor substrate 1 (IRS1), tyrosine-specific protein phosphatase 1B (PTP1B), Akt, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) and eNOS were evaluated. Fructose overload increased visceral adipose tissue, insulin concentration and homeostasis model assessment index. Exercise managed to decrease visceral adiposity and insulin level and to increase insulin sensitivity. Fructose diet increased level of cardiac PTP1B and pIRS1 (Ser307), while levels of IR and ERK1/2, as well as pIRS1 (Tyr 632), pAkt (Ser473, Thr308) and pERK1/2 were decreased. These disturbances were accompanied by reduced phosphorylation of eNOS at Ser1177. Exercise managed to prevent most of the disturbances in insulin signaling caused by fructose diet (except phosphorylation of IRS1 at Tyr 632 and phosphorylation and protein expression of ERK1/2) and consequently restored function of eNOS. Low intensity exercise could be considered as efficient treatment of cardiac insulin resistance induced by fructose diet. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
journal = "Molecular and Cellular Endocrinology",
title = "Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet",
volume = "420",
number = "C",
pages = "97-104",
doi = "10.1016/j.mce.2015.11.032"
}
Stanišić, J., Korićanac, G., Ćulafić, T., Romić, S. Đ., Stojiljković, M. D., Kostić, M., Pantelić, M.,& Tepavčević, S. (2016). Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet.
Molecular and Cellular Endocrinology, 420(C), 97-104.
https://doi.org/10.1016/j.mce.2015.11.032
Stanišić J, Korićanac G, Ćulafić T, Romić SĐ, Stojiljković MD, Kostić M, Pantelić M, Tepavčević S. Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet. Molecular and Cellular Endocrinology. 2016;420(C):97-104
Stanišić Jelena, Korićanac Goran, Ćulafić Tijana, Romić Snježana Đ., Stojiljković Mojca D., Kostić Milan, Pantelić Marija, Tepavčević Snežana, "Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet" Molecular and Cellular Endocrinology, 420, no. C (2016):97-104,
https://doi.org/10.1016/j.mce.2015.11.032 .
2
16
14
11

Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta

Bundalo, Maja M.; Živković, Maja; Romić, Snježana Đ.; Tepavčević, Snežana; Korićanac, Goran; Đurić, Tamara; Stanković, Aleksandra

(2016)

TY  - JOUR
AU  - Bundalo, Maja M.
AU  - Živković, Maja
AU  - Romić, Snježana Đ.
AU  - Tepavčević, Snežana
AU  - Korićanac, Goran
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1132
AB  - Introduction: The cardiovascular renin-angiotensin system (RAS) could be affected by gender and dietary regime. We hypothesized that male rats will be more susceptible to activation of RAS in the heart and aorta, as a response to a fructose-rich diet (FRD). Materials and methods: Both male and female Wistar rats were given a 10% (w/v) fructose solution for 9 weeks. We measured the biochemical parameters, blood pressure (BP) and heart rate. We used Western blot and real-time polymerase chain reaction (PCR) to quantify protein and gene expression. Results: In the male rats, the FRD elevated BP and expression of cardiac angiotensin-converting enzyme (ACE), while the expression of angiotensin-converting enzyme 2 (ACE2) and angiotensin II Type 2 receptor (AT(2)R) were significantly decreased. In female rats, there were no changes in cardiac RAS expression due to FRD. Furthermore, the ACE/AT(1)R axis was overexpressed in the FRD male rats aortae, while only AT(1)R was upregulated in the FRD female rats aortae. ACE2 expression remained unchanged in the aortae of both genders receiving the FRD. Conclusions: The FRD induced gender-specific changes in the expression of the RAS in the heart and aortae of male rats. Further investigations are required in order to get a comprehensive understanding of the underlying mechanisms of gender-specific fructose-induced cardiovascular pathologies.
T2  - Journal of the Renin-Angiotensin-Aldosterone System
T1  - Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta
VL  - 17
IS  - 2
DO  - 10.1177/1470320316642915
ER  - 
@article{
author = "Bundalo, Maja M. and Živković, Maja and Romić, Snježana Đ. and Tepavčević, Snežana and Korićanac, Goran and Đurić, Tamara and Stanković, Aleksandra",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1132",
abstract = "Introduction: The cardiovascular renin-angiotensin system (RAS) could be affected by gender and dietary regime. We hypothesized that male rats will be more susceptible to activation of RAS in the heart and aorta, as a response to a fructose-rich diet (FRD). Materials and methods: Both male and female Wistar rats were given a 10% (w/v) fructose solution for 9 weeks. We measured the biochemical parameters, blood pressure (BP) and heart rate. We used Western blot and real-time polymerase chain reaction (PCR) to quantify protein and gene expression. Results: In the male rats, the FRD elevated BP and expression of cardiac angiotensin-converting enzyme (ACE), while the expression of angiotensin-converting enzyme 2 (ACE2) and angiotensin II Type 2 receptor (AT(2)R) were significantly decreased. In female rats, there were no changes in cardiac RAS expression due to FRD. Furthermore, the ACE/AT(1)R axis was overexpressed in the FRD male rats aortae, while only AT(1)R was upregulated in the FRD female rats aortae. ACE2 expression remained unchanged in the aortae of both genders receiving the FRD. Conclusions: The FRD induced gender-specific changes in the expression of the RAS in the heart and aortae of male rats. Further investigations are required in order to get a comprehensive understanding of the underlying mechanisms of gender-specific fructose-induced cardiovascular pathologies.",
journal = "Journal of the Renin-Angiotensin-Aldosterone System",
title = "Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta",
volume = "17",
number = "2",
doi = "10.1177/1470320316642915"
}
Bundalo, M. M., Živković, M., Romić, S. Đ., Tepavčević, S., Korićanac, G., Đurić, T.,& Stanković, A. (2016). Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta.
Journal of the Renin-Angiotensin-Aldosterone System, 17(2).
https://doi.org/10.1177/1470320316642915
Bundalo MM, Živković M, Romić SĐ, Tepavčević S, Korićanac G, Đurić T, Stanković A. Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta. Journal of the Renin-Angiotensin-Aldosterone System. 2016;17(2)
Bundalo Maja M., Živković Maja, Romić Snježana Đ., Tepavčević Snežana, Korićanac Goran, Đurić Tamara, Stanković Aleksandra, "Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta" Journal of the Renin-Angiotensin-Aldosterone System, 17, no. 2 (2016),
https://doi.org/10.1177/1470320316642915 .
17
14
16

Posttraumatic and depressive symptoms in beta-endorphin dynamics

Savić, Danka A.; Knežević, Goran; Matić, Gordana; Damjanović, Svetozar S.; Spiric, Zeljko

(2015)

TY  - JOUR
AU  - Savić, Danka A.
AU  - Knežević, Goran
AU  - Matić, Gordana
AU  - Damjanović, Svetozar S.
AU  - Spiric, Zeljko
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/554
AB  - A disturbed beta-endorphin system can be a part of the post-traumatic stress disorder (PTSD) and depression allostasis. Study subjects (N=392) included those with PTSD and/or (stress-induced) depression, and healthy controls with and without traumas. The aim of the study was to examine the network of relations centered around plasma beta-endorphin. The network included anxiety (as a personality trait), traumatic events, pain, aggressiveness, depressive symptoms, and three clusters of PTSD symptoms: intrusions, avoidance, and hyperarousal. Beta-endorphin was represented by individual mean from 13 time points (BEmean), reflecting the total amount of the peripherally secreted hormone, and the coefficient of variation (BEvar), calculated as the ratio of standard deviation to the mean, reflecting the hormones dynamics. BEvar correlated with all other variables, BEmean had no correlations. Structural equation modeling (SEM) was used to examine all interrelations (including their directions) of BEvar and the state/trait variables in the context of their entirety. The model revealed that hyperarousal and anxiety were the only direct agents of peripheral beta-endorphin fluctuations, mediating the effects of other variables. Traumatic events and intrusions act on BEvar via hyperarousal, while depressive symptoms, avoidance, and pain act via anxiety. Hyperarousal should be emphasized as the main agent not only because its effect on BEvar is larger than that of anxiety, but also because it increases anxiety itself (via avoidance and pain). All influences on BEvar are positive and they indicate long-term (sensitizing) effects (as opposed to direct stimulation, for example, by acute pain, anger, etc.). Relations apart from beta-endorphin are also discussed. (C) 2015 Elsevier B.V. All rights reserved,
T2  - Journal of Affective Disorders
T1  - Posttraumatic and depressive symptoms in beta-endorphin dynamics
VL  - 181
SP  - 61
EP  - 66
DO  - 10.1016/j.jad.2015.03.063
ER  - 
@article{
author = "Savić, Danka A. and Knežević, Goran and Matić, Gordana and Damjanović, Svetozar S. and Spiric, Zeljko",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/554",
abstract = "A disturbed beta-endorphin system can be a part of the post-traumatic stress disorder (PTSD) and depression allostasis. Study subjects (N=392) included those with PTSD and/or (stress-induced) depression, and healthy controls with and without traumas. The aim of the study was to examine the network of relations centered around plasma beta-endorphin. The network included anxiety (as a personality trait), traumatic events, pain, aggressiveness, depressive symptoms, and three clusters of PTSD symptoms: intrusions, avoidance, and hyperarousal. Beta-endorphin was represented by individual mean from 13 time points (BEmean), reflecting the total amount of the peripherally secreted hormone, and the coefficient of variation (BEvar), calculated as the ratio of standard deviation to the mean, reflecting the hormones dynamics. BEvar correlated with all other variables, BEmean had no correlations. Structural equation modeling (SEM) was used to examine all interrelations (including their directions) of BEvar and the state/trait variables in the context of their entirety. The model revealed that hyperarousal and anxiety were the only direct agents of peripheral beta-endorphin fluctuations, mediating the effects of other variables. Traumatic events and intrusions act on BEvar via hyperarousal, while depressive symptoms, avoidance, and pain act via anxiety. Hyperarousal should be emphasized as the main agent not only because its effect on BEvar is larger than that of anxiety, but also because it increases anxiety itself (via avoidance and pain). All influences on BEvar are positive and they indicate long-term (sensitizing) effects (as opposed to direct stimulation, for example, by acute pain, anger, etc.). Relations apart from beta-endorphin are also discussed. (C) 2015 Elsevier B.V. All rights reserved,",
journal = "Journal of Affective Disorders",
title = "Posttraumatic and depressive symptoms in beta-endorphin dynamics",
volume = "181",
pages = "61-66",
doi = "10.1016/j.jad.2015.03.063"
}
Savić, D. A., Knežević, G., Matić, G., Damjanović, S. S.,& Spiric, Z. (2015). Posttraumatic and depressive symptoms in beta-endorphin dynamics.
Journal of Affective Disorders, 181, 61-66.
https://doi.org/10.1016/j.jad.2015.03.063
Savić DA, Knežević G, Matić G, Damjanović SS, Spiric Z. Posttraumatic and depressive symptoms in beta-endorphin dynamics. Journal of Affective Disorders. 2015;181:61-66
Savić Danka A., Knežević Goran, Matić Gordana, Damjanović Svetozar S., Spiric Zeljko, "Posttraumatic and depressive symptoms in beta-endorphin dynamics" Journal of Affective Disorders, 181 (2015):61-66,
https://doi.org/10.1016/j.jad.2015.03.063 .
1
8
8
10

Efekti estradiola u srcu pacova sa insulinskom rezistencijom izazvanom ishranom bogatom fruktozom

Romić, Snježana Đ.

(Универзитет у Београду, Биолошки факултет, 2015)

TY  - BOOK
AU  - Romić, Snježana Đ.
PY  - 2015
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=2310
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:10216/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024953522
UR  - http://nardus.mpn.gov.rs/123456789/4170
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7272
AB  - Fruktoza je prirodni šećer, ĉiji se unos poslednjih decenija dramatiĉno povećao, najvećim delom zbog povećane upotrebe kukuruznog sirupa sa visokim sadrţajem fruktoze. Povećan unos fruktoze vodi ka razvoju metaboliĉkog sindroma kod ljudi i eksperimentalnih ţivotinja. Pored promena u jetri, adipoznom tkivu i skeletnim mišićima, ishrana bogata fruktozom je praćena i razvojem insulinske rezistencije u srcu. Poznato je da insulin ostvaruje svoje efekte na ćelije srca i da u stanju insulinske rezistencije u srcu dolazi do poremećaja u signalnom putu Akt/eNOS i balansu korišćenja energetskih supstrata. Posledice povećanog unosa fruktoze su polno zavisne, pri ĉemu je protektivni efekat pripisan estrogenim hormonima. Fiziološke koncentracije estrogena poboljšavaju osetljivost na insulin i deluju kardioprotektivno. TakoĊe, estradiol utiĉe na specifiĉno delovanje insulina u srcu.Cilj ove studije bio je analiza efekta estradiola na procese u srcu regulisane insulinom u stanju insulinske rezistencije izazvane ishranom bogatom fruktozom. Ţenke pacova su podvrgnute standardnoj ishrani ili ishrani obogaćenoj 10% rastvorom fruktoze tokom 9 nedelja i ovarijektomisane 2 nedelje pre ţrtvovanja, pri ĉemu je polovina ţivotinja na fruktoznoj ishrani dan nakon ovarijektomije podvrgnuta tretmanu estradiolom. U cilju izuĉavanja efekata ishrane bogate fruktozom i estradiola na insulinom regulisanu fosforilaciju i unutarćelijsku lokalizaciju ispitivanih molekula, polovina ţivotinja je tretirana insulinom 40 minuta pre ţrtvovanja. Analizirani su molekuli iz insulinskog signalnog puta (IRS-1, Akt i ERK1/2), kao i efektorni molekuli ĉiju funkciju reguliše insulin, kao što su eNOS, Na+/K+-ATP-aza i molekuli ukljuĉeni u transport i metabolizam energetskih supstrata u srcu (GLUT1, GLUT4, CD36, lipin 1 i CPTI) i lipidni profil srca. Ispitivana je ekspresija ovih molekula na nivou proteina, kao i njihova fosforilacija ili unutarćelijska lokalizacija.Ishrana bogata fruktozom je povećala unos teĉnosti i smanjila unos hrane, dok je povećala ukupan kalorijski unos i koncentraciju leptina u plazmi. Estradiol je većim delom poništio štetne efekte ishrane na regulaciju apetita, verovatno preko povećanjacentralne osetljivosti na leptin. Ţivotinje na fruktoznoj ishrani su imale povišene koncentracije triglicerida i insulina u plazmi i povećan HOMA indeks, smanjenu koncentraciju slobodnih masnih kiselina, dok je koncentracija glukoze bila nepromenjena...
AB  - Fructose is natural sugar whose intake has increased dramatically over the past decades, mostly due to increased consumption of high-fructose corn syrup. Increased intake of fructose initiates development of metabolic syndrome phenotype in humans and experimental animals. In addition to changes in liver, adippose tissue and skeletal muscle, fructose-rich diеt is accompanied by cardiac insulin resistance. Cardiac muscle is a target of insulin. Insulin resistance is accompanied by disturbances in Akt/eNOS signalling and altered cardiac usage of energetic substrates. Consequences of enhanced fructose intake are shown to be sex-dependent and the protective effect is attributed to estrogens. Physiological concentrations of estrogens improve insulin sensitivity and they are cardioprotective. Also, estrogens affect specific cardiac insulin action.The aim of the present study was to analyze effects of estradiol on insulin-regulated processes in the heart in insulin resistance state. Female rats were subjected to standard diet or diet containing 10% fructose in drinking water during 9 weeks. Two weeks before sacrifice, all animals were bilaterally ovariectomized and half of the fructose-fed animals were subjected to estradiol replacement treatment, day after ovariectomy. In order to study fructose-rich diet and estradiol effects on the insulin regulated phosphorylations and subcellular localization of analyzed molecules, half of the animals were treated with insulin, 40 min before killing. Insulin signaling molecules (IRS-1, Akt i ERK1/2) were analyzed, as well as insulin regulated effector molecules, such as eNOS, Na+/K+-ATPase and molecules involved in the transport and metabolism of energetic substrates in the heart (GLUT1, GLUT4, CD36, lipin 1 i CPTI), as well as profile of cardiac lipids. We examined the protein exppression of the molecules, as well as their phosphorylation or subcellular localization.Fructose-rich diet increased liquid intake and decreased food intake, while it increased total caloric intake and plasma leptin concentration. Estradiol abolished most of the detrimental effects of diet on appetite regulation, probably through increase in central leptin sensitivity. The fructose-fed rats had increased plasma triglycerides andinsulin levels and increased HOMA index, decreased plasma free fatty acid level, while glucose concentration was unaltered...
PB  - Универзитет у Београду, Биолошки факултет
T2  - Универзитет у Београду
T1  - Efekti estradiola u srcu pacova sa insulinskom rezistencijom izazvanom ishranom bogatom fruktozom
T1  - Effects of estradiol in the heart of rats with fructose-rich diet-induced insulin resistance
ER  - 
@phdthesis{
author = "Romić, Snježana Đ.",
year = "2015",
url = "http://eteze.bg.ac.rs/application/showtheses?thesesId=2310, https://fedorabg.bg.ac.rs/fedora/get/o:10216/bdef:Content/download, http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024953522, http://nardus.mpn.gov.rs/123456789/4170, http://vinar.vin.bg.ac.rs/handle/123456789/7272",
abstract = "Fruktoza je prirodni šećer, ĉiji se unos poslednjih decenija dramatiĉno povećao, najvećim delom zbog povećane upotrebe kukuruznog sirupa sa visokim sadrţajem fruktoze. Povećan unos fruktoze vodi ka razvoju metaboliĉkog sindroma kod ljudi i eksperimentalnih ţivotinja. Pored promena u jetri, adipoznom tkivu i skeletnim mišićima, ishrana bogata fruktozom je praćena i razvojem insulinske rezistencije u srcu. Poznato je da insulin ostvaruje svoje efekte na ćelije srca i da u stanju insulinske rezistencije u srcu dolazi do poremećaja u signalnom putu Akt/eNOS i balansu korišćenja energetskih supstrata. Posledice povećanog unosa fruktoze su polno zavisne, pri ĉemu je protektivni efekat pripisan estrogenim hormonima. Fiziološke koncentracije estrogena poboljšavaju osetljivost na insulin i deluju kardioprotektivno. TakoĊe, estradiol utiĉe na specifiĉno delovanje insulina u srcu.Cilj ove studije bio je analiza efekta estradiola na procese u srcu regulisane insulinom u stanju insulinske rezistencije izazvane ishranom bogatom fruktozom. Ţenke pacova su podvrgnute standardnoj ishrani ili ishrani obogaćenoj 10% rastvorom fruktoze tokom 9 nedelja i ovarijektomisane 2 nedelje pre ţrtvovanja, pri ĉemu je polovina ţivotinja na fruktoznoj ishrani dan nakon ovarijektomije podvrgnuta tretmanu estradiolom. U cilju izuĉavanja efekata ishrane bogate fruktozom i estradiola na insulinom regulisanu fosforilaciju i unutarćelijsku lokalizaciju ispitivanih molekula, polovina ţivotinja je tretirana insulinom 40 minuta pre ţrtvovanja. Analizirani su molekuli iz insulinskog signalnog puta (IRS-1, Akt i ERK1/2), kao i efektorni molekuli ĉiju funkciju reguliše insulin, kao što su eNOS, Na+/K+-ATP-aza i molekuli ukljuĉeni u transport i metabolizam energetskih supstrata u srcu (GLUT1, GLUT4, CD36, lipin 1 i CPTI) i lipidni profil srca. Ispitivana je ekspresija ovih molekula na nivou proteina, kao i njihova fosforilacija ili unutarćelijska lokalizacija.Ishrana bogata fruktozom je povećala unos teĉnosti i smanjila unos hrane, dok je povećala ukupan kalorijski unos i koncentraciju leptina u plazmi. Estradiol je većim delom poništio štetne efekte ishrane na regulaciju apetita, verovatno preko povećanjacentralne osetljivosti na leptin. Ţivotinje na fruktoznoj ishrani su imale povišene koncentracije triglicerida i insulina u plazmi i povećan HOMA indeks, smanjenu koncentraciju slobodnih masnih kiselina, dok je koncentracija glukoze bila nepromenjena..., Fructose is natural sugar whose intake has increased dramatically over the past decades, mostly due to increased consumption of high-fructose corn syrup. Increased intake of fructose initiates development of metabolic syndrome phenotype in humans and experimental animals. In addition to changes in liver, adippose tissue and skeletal muscle, fructose-rich diеt is accompanied by cardiac insulin resistance. Cardiac muscle is a target of insulin. Insulin resistance is accompanied by disturbances in Akt/eNOS signalling and altered cardiac usage of energetic substrates. Consequences of enhanced fructose intake are shown to be sex-dependent and the protective effect is attributed to estrogens. Physiological concentrations of estrogens improve insulin sensitivity and they are cardioprotective. Also, estrogens affect specific cardiac insulin action.The aim of the present study was to analyze effects of estradiol on insulin-regulated processes in the heart in insulin resistance state. Female rats were subjected to standard diet or diet containing 10% fructose in drinking water during 9 weeks. Two weeks before sacrifice, all animals were bilaterally ovariectomized and half of the fructose-fed animals were subjected to estradiol replacement treatment, day after ovariectomy. In order to study fructose-rich diet and estradiol effects on the insulin regulated phosphorylations and subcellular localization of analyzed molecules, half of the animals were treated with insulin, 40 min before killing. Insulin signaling molecules (IRS-1, Akt i ERK1/2) were analyzed, as well as insulin regulated effector molecules, such as eNOS, Na+/K+-ATPase and molecules involved in the transport and metabolism of energetic substrates in the heart (GLUT1, GLUT4, CD36, lipin 1 i CPTI), as well as profile of cardiac lipids. We examined the protein exppression of the molecules, as well as their phosphorylation or subcellular localization.Fructose-rich diet increased liquid intake and decreased food intake, while it increased total caloric intake and plasma leptin concentration. Estradiol abolished most of the detrimental effects of diet on appetite regulation, probably through increase in central leptin sensitivity. The fructose-fed rats had increased plasma triglycerides andinsulin levels and increased HOMA index, decreased plasma free fatty acid level, while glucose concentration was unaltered...",
publisher = "Универзитет у Београду, Биолошки факултет",
journal = "Универзитет у Београду",
title = "Efekti estradiola u srcu pacova sa insulinskom rezistencijom izazvanom ishranom bogatom fruktozom, Effects of estradiol in the heart of rats with fructose-rich diet-induced insulin resistance"
}
Romić, S. Đ. (2015). Effects of estradiol in the heart of rats with fructose-rich diet-induced insulin resistance.
Универзитет у Београду
Универзитет у Београду, Биолошки факултет..
Romić SĐ. Effects of estradiol in the heart of rats with fructose-rich diet-induced insulin resistance. Универзитет у Београду. 2015;
Romić Snježana Đ., "Effects of estradiol in the heart of rats with fructose-rich diet-induced insulin resistance" Универзитет у Београду (2015)

Oestradiol Treatment Counteracts the Effect of Fructose-Rich Diet on Matrix Metalloproteinase 9 Expression and NF kappa B Activation

Bundalo, Maja M.; Živković, Maja; Ćulafić, Tijana; Stojiljković, Mojca D.; Korićanac, Goran; Stanković, Aleksandra

(2015)

TY  - JOUR
AU  - Bundalo, Maja M.
AU  - Živković, Maja
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca D.
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1112
AB  - Fructose-rich diet induces metabolic changes similar to those observed in metabolic syndrome. Among other matrix metalloproteinases, MMP-9 has an important role in adverse cardiac remodelling and might have a role in the development of cardiovascular disorders associated with metabolic syndrome. The changes of MMP-9 expression could be mediated via the NF kappa B pathway. In this study we investigated the effect of fructose-rich diet on MMP-9 expression in the heart of male and female rats, along with the effect of fructose-rich diet and oestradiol on MMP-9 expression in ovariectomized females. We further assessed the effect of fructose-rich diet and oestradiol on NF kappa B activation, measured as the level of p65 phosphorylation at Ser 276. The results showed that the diet regime did not affect the heart mass. Higher MMP-9 gene expression was found in cardiac tissue of male rats fed the fructose-rich diet than in females on the same diet regime. In ovariectomized females, fructose-rich diet upregulated MMP-9 protein and mRNA expression in the heart, as well as phosphorylation of the p65 subunit of NF kappa B at Ser 276. Oestradiol replacement therapy reverted these changes in the heart of ovariectomized females. This study has shown that oestradiol could revert the early molecular changes in MMP-9 expression induced by fructose-rich diet that occurred before cardiac hypertrophy development by decreasing phosphorylation of the NF kappa B p65 subunit at Ser 276.
T2  - Folia Biologica
T1  - Oestradiol Treatment Counteracts the Effect of Fructose-Rich Diet on Matrix Metalloproteinase 9 Expression and NF kappa B Activation
VL  - 61
IS  - 6
SP  - 233
EP  - 240
ER  - 
@article{
author = "Bundalo, Maja M. and Živković, Maja and Ćulafić, Tijana and Stojiljković, Mojca D. and Korićanac, Goran and Stanković, Aleksandra",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1112",
abstract = "Fructose-rich diet induces metabolic changes similar to those observed in metabolic syndrome. Among other matrix metalloproteinases, MMP-9 has an important role in adverse cardiac remodelling and might have a role in the development of cardiovascular disorders associated with metabolic syndrome. The changes of MMP-9 expression could be mediated via the NF kappa B pathway. In this study we investigated the effect of fructose-rich diet on MMP-9 expression in the heart of male and female rats, along with the effect of fructose-rich diet and oestradiol on MMP-9 expression in ovariectomized females. We further assessed the effect of fructose-rich diet and oestradiol on NF kappa B activation, measured as the level of p65 phosphorylation at Ser 276. The results showed that the diet regime did not affect the heart mass. Higher MMP-9 gene expression was found in cardiac tissue of male rats fed the fructose-rich diet than in females on the same diet regime. In ovariectomized females, fructose-rich diet upregulated MMP-9 protein and mRNA expression in the heart, as well as phosphorylation of the p65 subunit of NF kappa B at Ser 276. Oestradiol replacement therapy reverted these changes in the heart of ovariectomized females. This study has shown that oestradiol could revert the early molecular changes in MMP-9 expression induced by fructose-rich diet that occurred before cardiac hypertrophy development by decreasing phosphorylation of the NF kappa B p65 subunit at Ser 276.",
journal = "Folia Biologica",
title = "Oestradiol Treatment Counteracts the Effect of Fructose-Rich Diet on Matrix Metalloproteinase 9 Expression and NF kappa B Activation",
volume = "61",
number = "6",
pages = "233-240"
}
Bundalo, M. M., Živković, M., Ćulafić, T., Stojiljković, M. D., Korićanac, G.,& Stanković, A. (2015). Oestradiol Treatment Counteracts the Effect of Fructose-Rich Diet on Matrix Metalloproteinase 9 Expression and NF kappa B Activation.
Folia Biologica, 61(6), 233-240.
Bundalo MM, Živković M, Ćulafić T, Stojiljković MD, Korićanac G, Stanković A. Oestradiol Treatment Counteracts the Effect of Fructose-Rich Diet on Matrix Metalloproteinase 9 Expression and NF kappa B Activation. Folia Biologica. 2015;61(6):233-240
Bundalo Maja M., Živković Maja, Ćulafić Tijana, Stojiljković Mojca D., Korićanac Goran, Stanković Aleksandra, "Oestradiol Treatment Counteracts the Effect of Fructose-Rich Diet on Matrix Metalloproteinase 9 Expression and NF kappa B Activation" Folia Biologica, 61, no. 6 (2015):233-240
7
6

Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome

Tepavčević, Snežana; Milutinovic, Danijela Vojnovic; Macut, Djuro; Stojiljković, Mojca D.; Nikolic, Marina; Bozic-Antic, Ivana; Ćulafić, Tijana; Bjekic-Macut, Jelica; Matić, Gordana; Korićanac, Goran

(2015)

TY  - JOUR
AU  - Tepavčević, Snežana
AU  - Milutinovic, Danijela Vojnovic
AU  - Macut, Djuro
AU  - Stojiljković, Mojca D.
AU  - Nikolic, Marina
AU  - Bozic-Antic, Ivana
AU  - Ćulafić, Tijana
AU  - Bjekic-Macut, Jelica
AU  - Matić, Gordana
AU  - Korićanac, Goran
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/710
AB  - Polycystic ovary syndrome (PCOS) is associated with an altered plasma lipid profile and increased risk for cardiovascular diseases. We hypothesized that molecular mechanisms underlying cardiac pathology in PCOS involve changes in expression and subcellular localization of several key proteins involved in cardiac lipid transport and metabolism, such as fatty acid transporter CD36, lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 (PGC1), and carnitine palmitoyltransferase 1 (CPT1). We used the animal model of PCOS obtained by treating female rats with dihydrotestosterone (DHT). Protein levels of CD36, lipin 1, PPAR alpha, PGC1, and antioxidative enzymes were assessed by Western blot in different cardiac cell compartments. Cardiac triglycerides (TG) and lipid peroxidation were also measured. The content of CD36 was decreased in both the cardiac plasma membranes and intracellular pool. On the other hand, total content of cardiac lipin 1 in DHT-treated rats was elevated, in contrast to decreased microsomal lipin 1 content. An increase in nuclear content of lipin 1 was observed together with elevation of nuclear PPAR alpha and PGC1, and an increase in CPT1 expression. However, lipid peroxidation was reduced in the heart, without alterations in antioxidative enzymes expression and cardiac TG content. The results indicate that treatment of female rats with DHT is accompanied by a decrease of fatty acid uptake and a reduction of lipid peroxidation in the heart. The observed elevation of lipin 1, PPAR alpha, PGC1, and CPT1 expression suggests that cardiac fatty acid metabolism is shifted toward mitochondrial beta oxidation.
T2  - Endocrine
T1  - Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome
VL  - 50
IS  - 1
SP  - 193
EP  - 201
DO  - 10.1007/s12020-015-0558-1
ER  - 
@article{
author = "Tepavčević, Snežana and Milutinovic, Danijela Vojnovic and Macut, Djuro and Stojiljković, Mojca D. and Nikolic, Marina and Bozic-Antic, Ivana and Ćulafić, Tijana and Bjekic-Macut, Jelica and Matić, Gordana and Korićanac, Goran",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/710",
abstract = "Polycystic ovary syndrome (PCOS) is associated with an altered plasma lipid profile and increased risk for cardiovascular diseases. We hypothesized that molecular mechanisms underlying cardiac pathology in PCOS involve changes in expression and subcellular localization of several key proteins involved in cardiac lipid transport and metabolism, such as fatty acid transporter CD36, lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 (PGC1), and carnitine palmitoyltransferase 1 (CPT1). We used the animal model of PCOS obtained by treating female rats with dihydrotestosterone (DHT). Protein levels of CD36, lipin 1, PPAR alpha, PGC1, and antioxidative enzymes were assessed by Western blot in different cardiac cell compartments. Cardiac triglycerides (TG) and lipid peroxidation were also measured. The content of CD36 was decreased in both the cardiac plasma membranes and intracellular pool. On the other hand, total content of cardiac lipin 1 in DHT-treated rats was elevated, in contrast to decreased microsomal lipin 1 content. An increase in nuclear content of lipin 1 was observed together with elevation of nuclear PPAR alpha and PGC1, and an increase in CPT1 expression. However, lipid peroxidation was reduced in the heart, without alterations in antioxidative enzymes expression and cardiac TG content. The results indicate that treatment of female rats with DHT is accompanied by a decrease of fatty acid uptake and a reduction of lipid peroxidation in the heart. The observed elevation of lipin 1, PPAR alpha, PGC1, and CPT1 expression suggests that cardiac fatty acid metabolism is shifted toward mitochondrial beta oxidation.",
journal = "Endocrine",
title = "Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome",
volume = "50",
number = "1",
pages = "193-201",
doi = "10.1007/s12020-015-0558-1"
}
Tepavčević, S., Milutinovic, D. V., Macut, D., Stojiljković, M. D., Nikolic, M., Bozic-Antic, I., Ćulafić, T., Bjekic-Macut, J., Matić, G.,& Korićanac, G. (2015). Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome.
Endocrine, 50(1), 193-201.
https://doi.org/10.1007/s12020-015-0558-1
Tepavčević S, Milutinovic DV, Macut D, Stojiljković MD, Nikolic M, Bozic-Antic I, Ćulafić T, Bjekic-Macut J, Matić G, Korićanac G. Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome. Endocrine. 2015;50(1):193-201
Tepavčević Snežana, Milutinovic Danijela Vojnovic, Macut Djuro, Stojiljković Mojca D., Nikolic Marina, Bozic-Antic Ivana, Ćulafić Tijana, Bjekic-Macut Jelica, Matić Gordana, Korićanac Goran, "Cardiac fatty acid uptake and metabolism in the rat model of polycystic ovary syndrome" Endocrine, 50, no. 1 (2015):193-201,
https://doi.org/10.1007/s12020-015-0558-1 .
6
4
3

Fructose-Rich Diet-Induced Changes in the Expression of the Renin Angiotensin System Molecules in the Heart of Ovariectomized Female Rats Could be Reversed by Estradiol

Bundalo, Maja M.; Živković, Maja; Tepavčević, Snežana; Ćulafić, Tijana; Korićanac, Goran; Stanković, Aleksandra

(2015)

TY  - JOUR
AU  - Bundalo, Maja M.
AU  - Živković, Maja
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/570
AB  - The renin-angiotensin system has been implicated in the development of metabolic syndrome and appears to be a key in the local tissue control of normal cardiac functions. Physiological concentrations of estrogens have been shown to be cardioprotective, especially against the damaging effects of fructose-rich diet. The aim of the study was to investigate the expression of the renin-angiotensin system molecules with potentially deleterious effect on the heart (angiotensin-converting enzyme and angiotensin II type 1 receptor) and those with potentially protective effects, (angiotensin-converting enzyme 2 and angiotensin II type 2 receptor), in ovariectomized fructose fed female rats with 17-estradiol replacement. Real-time PCR and Western blot analysis were used for quantification of gene and protein expression in the heart. Fructose diet increased the expression of angiotensin-converting enzyme and angiotensin II type 1 receptor and decreased the expression of angiotensin-converting enzyme 2 and angiotensin II type 2 receptor. On the other hand, estradiol replacement seems to undo fructose diet effects on cardiac renin-angiotensin system. Downregulation of angiotensin-converting enzyme and angiotensin II type 1 receptor, and reversion of expression of both potentially protective molecules, angiotensin-converting enzyme 2 and angiotensin II type 2 receptor, to the control level in cardiac tissue took place. Obtained results suggest that estradiol may reverse the harmful effect of fructose-rich diet on the expression of renin-angiotensin system molecules. These findings may also be important in further research of phenotypes like insulin resistance, metabolic syndrome, and following cardiovascular pathology in females.
T2  - Hormone and Metabolic Research
T1  - Fructose-Rich Diet-Induced Changes in the Expression of the Renin Angiotensin System Molecules in the Heart of Ovariectomized Female Rats Could be Reversed by Estradiol
VL  - 47
IS  - 7
SP  - 521
EP  - 527
DO  - 10.1055/s-0034-1394373
ER  - 
@article{
author = "Bundalo, Maja M. and Živković, Maja and Tepavčević, Snežana and Ćulafić, Tijana and Korićanac, Goran and Stanković, Aleksandra",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/570",
abstract = "The renin-angiotensin system has been implicated in the development of metabolic syndrome and appears to be a key in the local tissue control of normal cardiac functions. Physiological concentrations of estrogens have been shown to be cardioprotective, especially against the damaging effects of fructose-rich diet. The aim of the study was to investigate the expression of the renin-angiotensin system molecules with potentially deleterious effect on the heart (angiotensin-converting enzyme and angiotensin II type 1 receptor) and those with potentially protective effects, (angiotensin-converting enzyme 2 and angiotensin II type 2 receptor), in ovariectomized fructose fed female rats with 17-estradiol replacement. Real-time PCR and Western blot analysis were used for quantification of gene and protein expression in the heart. Fructose diet increased the expression of angiotensin-converting enzyme and angiotensin II type 1 receptor and decreased the expression of angiotensin-converting enzyme 2 and angiotensin II type 2 receptor. On the other hand, estradiol replacement seems to undo fructose diet effects on cardiac renin-angiotensin system. Downregulation of angiotensin-converting enzyme and angiotensin II type 1 receptor, and reversion of expression of both potentially protective molecules, angiotensin-converting enzyme 2 and angiotensin II type 2 receptor, to the control level in cardiac tissue took place. Obtained results suggest that estradiol may reverse the harmful effect of fructose-rich diet on the expression of renin-angiotensin system molecules. These findings may also be important in further research of phenotypes like insulin resistance, metabolic syndrome, and following cardiovascular pathology in females.",
journal = "Hormone and Metabolic Research",
title = "Fructose-Rich Diet-Induced Changes in the Expression of the Renin Angiotensin System Molecules in the Heart of Ovariectomized Female Rats Could be Reversed by Estradiol",
volume = "47",
number = "7",
pages = "521-527",
doi = "10.1055/s-0034-1394373"
}
Bundalo, M. M., Živković, M., Tepavčević, S., Ćulafić, T., Korićanac, G.,& Stanković, A. (2015). Fructose-Rich Diet-Induced Changes in the Expression of the Renin Angiotensin System Molecules in the Heart of Ovariectomized Female Rats Could be Reversed by Estradiol.
Hormone and Metabolic Research, 47(7), 521-527.
https://doi.org/10.1055/s-0034-1394373
Bundalo MM, Živković M, Tepavčević S, Ćulafić T, Korićanac G, Stanković A. Fructose-Rich Diet-Induced Changes in the Expression of the Renin Angiotensin System Molecules in the Heart of Ovariectomized Female Rats Could be Reversed by Estradiol. Hormone and Metabolic Research. 2015;47(7):521-527
Bundalo Maja M., Živković Maja, Tepavčević Snežana, Ćulafić Tijana, Korićanac Goran, Stanković Aleksandra, "Fructose-Rich Diet-Induced Changes in the Expression of the Renin Angiotensin System Molecules in the Heart of Ovariectomized Female Rats Could be Reversed by Estradiol" Hormone and Metabolic Research, 47, no. 7 (2015):521-527,
https://doi.org/10.1055/s-0034-1394373 .
8
8
8

Cardiac Nitric Oxide Synthases and Na+/K+-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosterone

Tepavčević, Snežana; Milutinovic, D. V.; Macut, D.; Stanišić, Jelena; Nikolic, M.; Bozic-Antic, I.; Rodaljevic, S.; Bjekic-Macut, J.; Matić, Gordana; Korićanac, Goran

(2015)

TY  - JOUR
AU  - Tepavčević, Snežana
AU  - Milutinovic, D. V.
AU  - Macut, D.
AU  - Stanišić, Jelena
AU  - Nikolic, M.
AU  - Bozic-Antic, I.
AU  - Rodaljevic, S.
AU  - Bjekic-Macut, J.
AU  - Matić, Gordana
AU  - Korićanac, Goran
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/553
AB  - Nitric oxide synthases (NOSs) and Na+/K+-ATPase are enzymes essential for regular functioning of the heart. Since both enzymes are under insulin and androgen regulation and since insulin action and androgen level were disturbed in polycystic ovary syndrome (PCOS), we hypothesized that cardiac nitric oxide (NO) production and sodium/potassium transport would be deteriorated in PCOS. To test our hypothesis we introduced animal model of PCOS based on dihydrotestosterone (DHT) treatment of female Wistar rats and analyzed protein expression, phosphorylation or subcellular localization of endothelial NOS (eNOS), inducible NOS (iNOS) and alpha subunits of Na+/K+-ATPase in the heart. Obtained results indicate that DHT treatment significantly decreased cardiac eNOS protein level and activating phosphorylation at serine 1177, while inhibitory phosphorylation at threonine 495 was increased. In contrast to expression of eNOS, iNOS protein level in the heart of DHT-treated rats was significantly elevated. Furthermore, cardiac protein level of alpha 1 subunit of the ATPase, as well as its plasma membrane content, were decreased in rats with PCOS. In line with this, alpha 2 subunit protein level in fraction of plasma membranes was also significantly below control level. In conclusion, DHT treatment impaired effectiveness of NOSs and Na+/K+-ATPase in the female rat heart. Regarding the importance of NO production and sodium/potassium transport in the cardiac contraction and blood flow regulation, it implicates strong consequences of PCOS for heart functioning.
T2  - Experimental and Clinical Endocrinology and Diabetes
T1  - Cardiac Nitric Oxide Synthases and Na+/K+-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosterone
VL  - 123
IS  - 5
SP  - 303
EP  - 307
DO  - 10.1055/s-0035-1548929
ER  - 
@article{
author = "Tepavčević, Snežana and Milutinovic, D. V. and Macut, D. and Stanišić, Jelena and Nikolic, M. and Bozic-Antic, I. and Rodaljevic, S. and Bjekic-Macut, J. and Matić, Gordana and Korićanac, Goran",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/553",
abstract = "Nitric oxide synthases (NOSs) and Na+/K+-ATPase are enzymes essential for regular functioning of the heart. Since both enzymes are under insulin and androgen regulation and since insulin action and androgen level were disturbed in polycystic ovary syndrome (PCOS), we hypothesized that cardiac nitric oxide (NO) production and sodium/potassium transport would be deteriorated in PCOS. To test our hypothesis we introduced animal model of PCOS based on dihydrotestosterone (DHT) treatment of female Wistar rats and analyzed protein expression, phosphorylation or subcellular localization of endothelial NOS (eNOS), inducible NOS (iNOS) and alpha subunits of Na+/K+-ATPase in the heart. Obtained results indicate that DHT treatment significantly decreased cardiac eNOS protein level and activating phosphorylation at serine 1177, while inhibitory phosphorylation at threonine 495 was increased. In contrast to expression of eNOS, iNOS protein level in the heart of DHT-treated rats was significantly elevated. Furthermore, cardiac protein level of alpha 1 subunit of the ATPase, as well as its plasma membrane content, were decreased in rats with PCOS. In line with this, alpha 2 subunit protein level in fraction of plasma membranes was also significantly below control level. In conclusion, DHT treatment impaired effectiveness of NOSs and Na+/K+-ATPase in the female rat heart. Regarding the importance of NO production and sodium/potassium transport in the cardiac contraction and blood flow regulation, it implicates strong consequences of PCOS for heart functioning.",
journal = "Experimental and Clinical Endocrinology and Diabetes",
title = "Cardiac Nitric Oxide Synthases and Na+/K+-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosterone",
volume = "123",
number = "5",
pages = "303-307",
doi = "10.1055/s-0035-1548929"
}
Tepavčević, S., Milutinovic, D. V., Macut, D., Stanišić, J., Nikolic, M., Bozic-Antic, I., Rodaljevic, S., Bjekic-Macut, J., Matić, G.,& Korićanac, G. (2015). Cardiac Nitric Oxide Synthases and Na+/K+-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosterone.
Experimental and Clinical Endocrinology and Diabetes, 123(5), 303-307.
https://doi.org/10.1055/s-0035-1548929
Tepavčević S, Milutinovic DV, Macut D, Stanišić J, Nikolic M, Bozic-Antic I, Rodaljevic S, Bjekic-Macut J, Matić G, Korićanac G. Cardiac Nitric Oxide Synthases and Na+/K+-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosterone. Experimental and Clinical Endocrinology and Diabetes. 2015;123(5):303-307
Tepavčević Snežana, Milutinovic D. V., Macut D., Stanišić Jelena, Nikolic M., Bozic-Antic I., Rodaljevic S., Bjekic-Macut J., Matić Gordana, Korićanac Goran, "Cardiac Nitric Oxide Synthases and Na+/K+-ATPase in the Rat Model of Polycystic Ovary Syndrome Induced by Dihydrotestosterone" Experimental and Clinical Endocrinology and Diabetes, 123, no. 5 (2015):303-307,
https://doi.org/10.1055/s-0035-1548929 .
5
3
4

Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats

Romić, Snježana Đ.; Tepavčević, Snežana; Žakula, Zorica; Milosavljević, Tijana; Kostić, Milan; Petković, Marijana; Korićanac, Goran

(2014)

TY  - JOUR
AU  - Romić, Snježana Đ.
AU  - Tepavčević, Snežana
AU  - Žakula, Zorica
AU  - Milosavljević, Tijana
AU  - Kostić, Milan
AU  - Petković, Marijana
AU  - Korićanac, Goran
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6053
AB  - To give new insight to alterations of cardiac lipid metabolism accompanied by a fructose-rich diet (FRD), rats of both sexes were exposed to 10 % fructose in drinking water during 9 weeks. The protein level and subcellular localization of the main regulators of cardiac lipid metabolism, such as lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1 alpha), carnitine palmitoyltransferase I (CPTI), and CD36 were studied. Caloric intake in fructose-fed rats (FFR) of both sexes was increased. Circulating triacylglyceroles (TAG) and non-esterified fatty acids were increased in male FFR, while females increased visceral adiposity and blood TAG. Total expression of lipin 1 in cardiac cell lysate and its cytosolic and microsomal level were increased in the hearts of male FFR. PPAR alpha and PGC-1 alpha content were decreased in the nuclear extract. In addition, cardiac deposition of TAG in male FFR was elevated, as well as inhibitory phosphorylation of insulin receptor substrate 1 (IRS-1). In contrast, in female FFR, lipin 1 level was increased in nuclear extract only, while overall CPTI expression and phosphorylation of IRS-1 at serine 307 were decreased. The results of our study suggest that fructose diet causes gender-dependent alterations in cardiac lipid metabolism. Potentially detrimental effects of FRD seem to be limited to male rats. Most of the observed changes might be a consequence of elevated expression and altered localization of lipin 1. Increased inhibitory phosphorylation of IRS-1 is possible link between cardiac lipid metabolism and insulin resistance in FFR.
T2  - Lipids
T1  - Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats
VL  - 49
IS  - 7
SP  - 655
EP  - 663
DO  - 10.1007/s11745-014-3909-4
ER  - 
@article{
author = "Romić, Snježana Đ. and Tepavčević, Snežana and Žakula, Zorica and Milosavljević, Tijana and Kostić, Milan and Petković, Marijana and Korićanac, Goran",
year = "2014",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/6053",
abstract = "To give new insight to alterations of cardiac lipid metabolism accompanied by a fructose-rich diet (FRD), rats of both sexes were exposed to 10 % fructose in drinking water during 9 weeks. The protein level and subcellular localization of the main regulators of cardiac lipid metabolism, such as lipin 1, peroxisome proliferator-activated receptor alpha (PPAR alpha), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1 alpha), carnitine palmitoyltransferase I (CPTI), and CD36 were studied. Caloric intake in fructose-fed rats (FFR) of both sexes was increased. Circulating triacylglyceroles (TAG) and non-esterified fatty acids were increased in male FFR, while females increased visceral adiposity and blood TAG. Total expression of lipin 1 in cardiac cell lysate and its cytosolic and microsomal level were increased in the hearts of male FFR. PPAR alpha and PGC-1 alpha content were decreased in the nuclear extract. In addition, cardiac deposition of TAG in male FFR was elevated, as well as inhibitory phosphorylation of insulin receptor substrate 1 (IRS-1). In contrast, in female FFR, lipin 1 level was increased in nuclear extract only, while overall CPTI expression and phosphorylation of IRS-1 at serine 307 were decreased. The results of our study suggest that fructose diet causes gender-dependent alterations in cardiac lipid metabolism. Potentially detrimental effects of FRD seem to be limited to male rats. Most of the observed changes might be a consequence of elevated expression and altered localization of lipin 1. Increased inhibitory phosphorylation of IRS-1 is possible link between cardiac lipid metabolism and insulin resistance in FFR.",
journal = "Lipids",
title = "Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats",
volume = "49",
number = "7",
pages = "655-663",
doi = "10.1007/s11745-014-3909-4"
}
Romić, S. Đ., Tepavčević, S., Žakula, Z., Milosavljević, T., Kostić, M., Petković, M.,& Korićanac, G. (2014). Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats.
Lipids, 49(7), 655-663.
https://doi.org/10.1007/s11745-014-3909-4
Romić SĐ, Tepavčević S, Žakula Z, Milosavljević T, Kostić M, Petković M, Korićanac G. Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats. Lipids. 2014;49(7):655-663
Romić Snježana Đ., Tepavčević Snežana, Žakula Zorica, Milosavljević Tijana, Kostić Milan, Petković Marijana, Korićanac Goran, "Gender Differences in the Expression and Cellular Localization of Lipin 1 in the Hearts of Fructose-Fed Rats" Lipids, 49, no. 7 (2014):655-663,
https://doi.org/10.1007/s11745-014-3909-4 .
5
5
5

GR gene BclI polymorphysm changes the path, but not the level, of dexamethasone-induced cortisol suppression

Savić, Danka A.; Knežević, Goran; Damjanović, Svetozar S.; Antic, Jadranka; Matić, Gordana

(2014)

TY  - JOUR
AU  - Savić, Danka A.
AU  - Knežević, Goran
AU  - Damjanović, Svetozar S.
AU  - Antic, Jadranka
AU  - Matić, Gordana
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/108
AB  - Background: The hypothalamo-pituitary-adrenocortical (HPA) axis self-regulation is achieved via cortisol binding to mineralocorticoid (MR) and glucocorticoid receptors (GR). It is often disturbed in mental disorders, particularly in those where traumatic stress has been implicated, such as posttraumatic stress disorder and depression. Although dexamethasone suppression test (DST) is often used as diagnostic aid, the findings still vary. In search of the factors influencing the DST outcome, we examined the glucocorticoicl receptor (GR) gene Bell polymorphism. Methods: A total of 229 male subjects were classified into three Bell groups: two groups with homozygous carriers (of the G allele, N=108, and of the C allele, N=26), and one with heterozygous carriers (N=95). Multiple hierarchical linear regression analysis was clone, where the dependent variable was the clexamethasone-inclucecl cortisol suppression, and predictors included receptor variables. The interactions of the count of Gs with the predictors were introduced to single out the effects of the G allele. Results: The means of all studied variables, including suppression, are statistically the same in the three groups. However, the mechanism of suppression involves MRs only in the G allele carriers. Limitations: The subjects were selected by criteria suited for the aim of the large project whose part is this study, hence the relatively small number of CC carriers. Also, we did not assess MR functional properties that would probably sharpen the results. Conclusion: Our finding that MRs participate in cortisol suppression in the G allele carriers suggests that research aimed at refining HPA axis-based therapy might require its adjustment for such patients., (C) 2014 Elsevier By. All rights reserved.
T2  - Journal of Affective Disorders
T1  - GR gene BclI polymorphysm changes the path, but not the level, of dexamethasone-induced cortisol suppression
VL  - 168
SP  - 1
EP  - 4
DO  - 10.1016/j.jad.2014.06.046
ER  - 
@article{
author = "Savić, Danka A. and Knežević, Goran and Damjanović, Svetozar S. and Antic, Jadranka and Matić, Gordana",
year = "2014",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/108",
abstract = "Background: The hypothalamo-pituitary-adrenocortical (HPA) axis self-regulation is achieved via cortisol binding to mineralocorticoid (MR) and glucocorticoid receptors (GR). It is often disturbed in mental disorders, particularly in those where traumatic stress has been implicated, such as posttraumatic stress disorder and depression. Although dexamethasone suppression test (DST) is often used as diagnostic aid, the findings still vary. In search of the factors influencing the DST outcome, we examined the glucocorticoicl receptor (GR) gene Bell polymorphism. Methods: A total of 229 male subjects were classified into three Bell groups: two groups with homozygous carriers (of the G allele, N=108, and of the C allele, N=26), and one with heterozygous carriers (N=95). Multiple hierarchical linear regression analysis was clone, where the dependent variable was the clexamethasone-inclucecl cortisol suppression, and predictors included receptor variables. The interactions of the count of Gs with the predictors were introduced to single out the effects of the G allele. Results: The means of all studied variables, including suppression, are statistically the same in the three groups. However, the mechanism of suppression involves MRs only in the G allele carriers. Limitations: The subjects were selected by criteria suited for the aim of the large project whose part is this study, hence the relatively small number of CC carriers. Also, we did not assess MR functional properties that would probably sharpen the results. Conclusion: Our finding that MRs participate in cortisol suppression in the G allele carriers suggests that research aimed at refining HPA axis-based therapy might require its adjustment for such patients., (C) 2014 Elsevier By. All rights reserved.",
journal = "Journal of Affective Disorders",
title = "GR gene BclI polymorphysm changes the path, but not the level, of dexamethasone-induced cortisol suppression",
volume = "168",
pages = "1-4",
doi = "10.1016/j.jad.2014.06.046"
}
Savić, D. A., Knežević, G., Damjanović, S. S., Antic, J.,& Matić, G. (2014). GR gene BclI polymorphysm changes the path, but not the level, of dexamethasone-induced cortisol suppression.
Journal of Affective Disorders, 168, 1-4.
https://doi.org/10.1016/j.jad.2014.06.046
Savić DA, Knežević G, Damjanović SS, Antic J, Matić G. GR gene BclI polymorphysm changes the path, but not the level, of dexamethasone-induced cortisol suppression. Journal of Affective Disorders. 2014;168:1-4
Savić Danka A., Knežević Goran, Damjanović Svetozar S., Antic Jadranka, Matić Gordana, "GR gene BclI polymorphysm changes the path, but not the level, of dexamethasone-induced cortisol suppression" Journal of Affective Disorders, 168 (2014):1-4,
https://doi.org/10.1016/j.jad.2014.06.046 .
3
3
3

Mineralocorticoid receptor and heat shock protein expression levels in peripheral lymphocytes from war trauma-exposed men with and without PTSD

Matić, Gordana; Milutinovic, Danijela Vojnovic; Nestorov, Jelena; Elakovic, Ivana; Jovanovic, Sanja Manitasevic; Elzaedi, Younis Mouftah; Perisic, Tatjana; Dunderski, Jadranka; Damjanović, Svetozar S.; Knežević, Goran; Spiric, Zeljko; Vermetten, Eric; Savić, Danka A.

(2014)

TY  - JOUR
AU  - Matić, Gordana
AU  - Milutinovic, Danijela Vojnovic
AU  - Nestorov, Jelena
AU  - Elakovic, Ivana
AU  - Jovanovic, Sanja Manitasevic
AU  - Elzaedi, Younis Mouftah
AU  - Perisic, Tatjana
AU  - Dunderski, Jadranka
AU  - Damjanović, Svetozar S.
AU  - Knežević, Goran
AU  - Spiric, Zeljko
AU  - Vermetten, Eric
AU  - Savić, Danka A.
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5909
AB  - Alterations in the number and functional status of mineralocorticoid (MR) and glucocorticoid receptors (GR) may contribute to vulnerability to posttraumatic stress disorder (PTSD). Corticosteroid receptors are chaperoned by heat shock proteins Hsp90 and Hsp70. We examined relations between corticosteroid receptor and heat shock protein expression levels, and related them with war trauma exposure, PTSD and resilience to PTSD. Relative levels of MR. Hsp90 and Hsp70 were determined by immunoblotting in lymphocytes from war trauma-exposed men with current PTSD (current PTSD group, n=113), with lifetime PTSD (life-time PTSD group, n=61) and without PTSD (trauma control group, n=88), and from non-traumatized healthy controls (healthy control group, n=85). Between-group differences in MR, Hsp90 and Hsp70 levels and in MR/GR ratio were not observed. The level of MR was correlated with both Hsp90 and Hsp70 levels in trauma control and healthy control groups. On the other hand, GR level was correlated only with Hsp90 level, and this correlation was evident in current PTSD and trauma control groups. In conclusion, PTSD and exposure to trauma are not related to changes in lymphocyte MR, Hsp90 or Hsp70 levels, but may be associated with disturbances in corticosteroid receptors interaction with heat shock proteins. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
T2  - Psychiatry Research
T1  - Mineralocorticoid receptor and heat shock protein expression levels in peripheral lymphocytes from war trauma-exposed men with and without PTSD
VL  - 215
IS  - 2
SP  - 379
EP  - 385
DO  - 10.1016/j.psychres.2013.11.022
ER  - 
@article{
author = "Matić, Gordana and Milutinovic, Danijela Vojnovic and Nestorov, Jelena and Elakovic, Ivana and Jovanovic, Sanja Manitasevic and Elzaedi, Younis Mouftah and Perisic, Tatjana and Dunderski, Jadranka and Damjanović, Svetozar S. and Knežević, Goran and Spiric, Zeljko and Vermetten, Eric and Savić, Danka A.",
year = "2014",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5909",
abstract = "Alterations in the number and functional status of mineralocorticoid (MR) and glucocorticoid receptors (GR) may contribute to vulnerability to posttraumatic stress disorder (PTSD). Corticosteroid receptors are chaperoned by heat shock proteins Hsp90 and Hsp70. We examined relations between corticosteroid receptor and heat shock protein expression levels, and related them with war trauma exposure, PTSD and resilience to PTSD. Relative levels of MR. Hsp90 and Hsp70 were determined by immunoblotting in lymphocytes from war trauma-exposed men with current PTSD (current PTSD group, n=113), with lifetime PTSD (life-time PTSD group, n=61) and without PTSD (trauma control group, n=88), and from non-traumatized healthy controls (healthy control group, n=85). Between-group differences in MR, Hsp90 and Hsp70 levels and in MR/GR ratio were not observed. The level of MR was correlated with both Hsp90 and Hsp70 levels in trauma control and healthy control groups. On the other hand, GR level was correlated only with Hsp90 level, and this correlation was evident in current PTSD and trauma control groups. In conclusion, PTSD and exposure to trauma are not related to changes in lymphocyte MR, Hsp90 or Hsp70 levels, but may be associated with disturbances in corticosteroid receptors interaction with heat shock proteins. (C) 2013 Elsevier Ireland Ltd. All rights reserved.",
journal = "Psychiatry Research",
title = "Mineralocorticoid receptor and heat shock protein expression levels in peripheral lymphocytes from war trauma-exposed men with and without PTSD",
volume = "215",
number = "2",
pages = "379-385",
doi = "10.1016/j.psychres.2013.11.022"
}
Matić, G., Milutinovic, D. V., Nestorov, J., Elakovic, I., Jovanovic, S. M., Elzaedi, Y. M., Perisic, T., Dunderski, J., Damjanović, S. S., Knežević, G., Spiric, Z., Vermetten, E.,& Savić, D. A. (2014). Mineralocorticoid receptor and heat shock protein expression levels in peripheral lymphocytes from war trauma-exposed men with and without PTSD.
Psychiatry Research, 215(2), 379-385.
https://doi.org/10.1016/j.psychres.2013.11.022
Matić G, Milutinovic DV, Nestorov J, Elakovic I, Jovanovic SM, Elzaedi YM, Perisic T, Dunderski J, Damjanović SS, Knežević G, Spiric Z, Vermetten E, Savić DA. Mineralocorticoid receptor and heat shock protein expression levels in peripheral lymphocytes from war trauma-exposed men with and without PTSD. Psychiatry Research. 2014;215(2):379-385
Matić Gordana, Milutinovic Danijela Vojnovic, Nestorov Jelena, Elakovic Ivana, Jovanovic Sanja Manitasevic, Elzaedi Younis Mouftah, Perisic Tatjana, Dunderski Jadranka, Damjanović Svetozar S., Knežević Goran, Spiric Zeljko, Vermetten Eric, Savić Danka A., "Mineralocorticoid receptor and heat shock protein expression levels in peripheral lymphocytes from war trauma-exposed men with and without PTSD" Psychiatry Research, 215, no. 2 (2014):379-385,
https://doi.org/10.1016/j.psychres.2013.11.022 .
12
11
10

Expression and Cellular Distribution of Glucose Transporters and Alpha Subunits of Na+/K+-ATPase in the Heart of Fructose-fed Female Rats: The Role of Estradiol

Korićanac, Goran; Tepavčević, Snežana; Romić, Snježana Đ.; Milosavljević, Tijana; Stojiljković, Mojca D.; Žakula, Zorica

(2014)

TY  - JOUR
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Romić, Snježana Đ.
AU  - Milosavljević, Tijana
AU  - Stojiljković, Mojca D.
AU  - Žakula, Zorica
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5879
T2  - Hormone and Metabolic Research
T1  - Expression and Cellular Distribution of Glucose Transporters and Alpha Subunits of Na+/K+-ATPase in the Heart of Fructose-fed Female Rats: The Role of Estradiol
VL  - 46
IS  - 2
SP  - 109
EP  - 115
ER  - 
@article{
author = "Korićanac, Goran and Tepavčević, Snežana and Romić, Snježana Đ. and Milosavljević, Tijana and Stojiljković, Mojca D. and Žakula, Zorica",
year = "2014",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5879",
journal = "Hormone and Metabolic Research",
title = "Expression and Cellular Distribution of Glucose Transporters and Alpha Subunits of Na+/K+-ATPase in the Heart of Fructose-fed Female Rats: The Role of Estradiol",
volume = "46",
number = "2",
pages = "109-115"
}
Korićanac, G., Tepavčević, S., Romić, S. Đ., Milosavljević, T., Stojiljković, M. D.,& Žakula, Z. (2014). Expression and Cellular Distribution of Glucose Transporters and Alpha Subunits of Na+/K+-ATPase in the Heart of Fructose-fed Female Rats: The Role of Estradiol.
Hormone and Metabolic Research, 46(2), 109-115.
Korićanac G, Tepavčević S, Romić SĐ, Milosavljević T, Stojiljković MD, Žakula Z. Expression and Cellular Distribution of Glucose Transporters and Alpha Subunits of Na+/K+-ATPase in the Heart of Fructose-fed Female Rats: The Role of Estradiol. Hormone and Metabolic Research. 2014;46(2):109-115
Korićanac Goran, Tepavčević Snežana, Romić Snježana Đ., Milosavljević Tijana, Stojiljković Mojca D., Žakula Zorica, "Expression and Cellular Distribution of Glucose Transporters and Alpha Subunits of Na+/K+-ATPase in the Heart of Fructose-fed Female Rats: The Role of Estradiol" Hormone and Metabolic Research, 46, no. 2 (2014):109-115
7

Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome

Tepavčević, Snežana; Milutinovic, Danijela Vojnovic; Macut, Djuro; Žakula, Zorica; Nikolic, Marina; Bozic-Antic, Ivana; Romić, Snježana Đ.; Bjekic-Macut, Jelica; Matić, Gordana; Korićanac, Goran

(2014)

TY  - JOUR
AU  - Tepavčević, Snežana
AU  - Milutinovic, Danijela Vojnovic
AU  - Macut, Djuro
AU  - Žakula, Zorica
AU  - Nikolic, Marina
AU  - Bozic-Antic, Ivana
AU  - Romić, Snježana Đ.
AU  - Bjekic-Macut, Jelica
AU  - Matić, Gordana
AU  - Korićanac, Goran
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5970
AB  - It is supposed that women with polycystic ovary syndrome (PCOS) are prone to develop cardiovascular disease as a consequence of multiple risk factors that are mostly related to the state of insulin resistance and consequent hyperinsulinemia. In the present study, we evaluated insulin signaling and glucose transporters (GLUT) in cardiac cells of dihydrotestosterone (DHT) treated female rats as an animal model of PCOS. Expression of proteins involved in cardiac insulin signaling pathways and glucose transporters, as well as their phosphorylation or intracellular localization were studied by Western blot analysis in DHT-treated and control rats. Treatment with DHT resulted in increased body mass, absolute mass of the heart, elevated plasma insulin concentration, dyslipidemia and insulin resistance. At the molecular level, DHT treatment did not change protein expression of cardiac insulin receptor and insulin receptor substrate 1, while phosphorylation of the substrate at serine 307 was increased. Unexpectedly, although expression of downstream Akt kinase and its phosphorylation at threonine 308 were not altered, phosphoiylation of Akt at serine 473 was increased in the heart of DHT-treated rats. In contrast, expression and phosphorylation of extracellular signal regulated kinases 1/2 were decreased. Plasma membrane contents of GLUT1 and GLUT4 were decreased, as well as the expression of GLUT4 in cardiac cells at the end of androgen treatment. The obtained results provide evidence for alterations in expression and especially in functional characteristics of insulin signaling molecules and glucose transporters in the heart of DHT-treated rats with PCOS, indicating impaired cardiac insulin action. (c) 2014 Elsevier Ltd. All rights reserved.
T2  - Journal of Steroid Biochemistry and Molecular Biology
T1  - Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome
VL  - 141
SP  - 71
EP  - 76
DO  - 10.1016/j.jsbmb.2014.01.006
ER  - 
@article{
author = "Tepavčević, Snežana and Milutinovic, Danijela Vojnovic and Macut, Djuro and Žakula, Zorica and Nikolic, Marina and Bozic-Antic, Ivana and Romić, Snježana Đ. and Bjekic-Macut, Jelica and Matić, Gordana and Korićanac, Goran",
year = "2014",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5970",
abstract = "It is supposed that women with polycystic ovary syndrome (PCOS) are prone to develop cardiovascular disease as a consequence of multiple risk factors that are mostly related to the state of insulin resistance and consequent hyperinsulinemia. In the present study, we evaluated insulin signaling and glucose transporters (GLUT) in cardiac cells of dihydrotestosterone (DHT) treated female rats as an animal model of PCOS. Expression of proteins involved in cardiac insulin signaling pathways and glucose transporters, as well as their phosphorylation or intracellular localization were studied by Western blot analysis in DHT-treated and control rats. Treatment with DHT resulted in increased body mass, absolute mass of the heart, elevated plasma insulin concentration, dyslipidemia and insulin resistance. At the molecular level, DHT treatment did not change protein expression of cardiac insulin receptor and insulin receptor substrate 1, while phosphorylation of the substrate at serine 307 was increased. Unexpectedly, although expression of downstream Akt kinase and its phosphorylation at threonine 308 were not altered, phosphoiylation of Akt at serine 473 was increased in the heart of DHT-treated rats. In contrast, expression and phosphorylation of extracellular signal regulated kinases 1/2 were decreased. Plasma membrane contents of GLUT1 and GLUT4 were decreased, as well as the expression of GLUT4 in cardiac cells at the end of androgen treatment. The obtained results provide evidence for alterations in expression and especially in functional characteristics of insulin signaling molecules and glucose transporters in the heart of DHT-treated rats with PCOS, indicating impaired cardiac insulin action. (c) 2014 Elsevier Ltd. All rights reserved.",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
title = "Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome",
volume = "141",
pages = "71-76",
doi = "10.1016/j.jsbmb.2014.01.006"
}
Tepavčević, S., Milutinovic, D. V., Macut, D., Žakula, Z., Nikolic, M., Bozic-Antic, I., Romić, S. Đ., Bjekic-Macut, J., Matić, G.,& Korićanac, G. (2014). Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome.
Journal of Steroid Biochemistry and Molecular Biology, 141, 71-76.
https://doi.org/10.1016/j.jsbmb.2014.01.006
Tepavčević S, Milutinovic DV, Macut D, Žakula Z, Nikolic M, Bozic-Antic I, Romić SĐ, Bjekic-Macut J, Matić G, Korićanac G. Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome. Journal of Steroid Biochemistry and Molecular Biology. 2014;141:71-76
Tepavčević Snežana, Milutinovic Danijela Vojnovic, Macut Djuro, Žakula Zorica, Nikolic Marina, Bozic-Antic Ivana, Romić Snježana Đ., Bjekic-Macut Jelica, Matić Gordana, Korićanac Goran, "Dihydrotestosterone deteriorates cardiac insulin signaling and glucose transport in the rat model of polycystic ovary syndrome" Journal of Steroid Biochemistry and Molecular Biology, 141 (2014):71-76,
https://doi.org/10.1016/j.jsbmb.2014.01.006 .
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Efekat eksperimentalnog dijabetesa tipa 1 i supstitucione terapije na molekule regulisane insulinom u srcu pacova

Stojiljković, Mojca D.

(Универзитет у Београду, Биолошки факултет, 2014)

TY  - BOOK
AU  - Stojiljković, Mojca D.
PY  - 2014
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=3811
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:12801/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024763058
UR  - http://nardus.mpn.gov.rs/123456789/6495
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/7302
AB  - Insulinska deficijencija i hiperglikemija, koje predstavljaju osnovnekarakteristike dijabetesa tipa 1, povezane su sa brojnim endokrinim imetaboličkim promenama, a jedna od najčešćih komplikacija je povećanaučestalost nastanka kardiovaskularnih oboljenja. Zbog toga je rana i adekvatnaprimena terapije insulinom neophodna, kako za kontrolu bolesti, tako i zaprevenciju potencijalnih komplikacija.Polazna hipoteza istraţivanja čiji su rezultati obuhvaćeni ovomdoktorskom disertacijom je bila da u stanju hipoinsulinemije i hiperglikemijemoţe doći do promena u signalnom putu insulina u srcu, što moţe da se odrazina produkciju azot-monoksida i iskorišćavanje energetskih supstrata, a da bisupstitucija insulinom trebala da koriguje nastale promene.Za uspostavljanje eksperimentalnog modela dijabetesa tipa 1, muţjacipacova soja Wistar tretirani su streptozotocinom (60 mg/kg). Poznato je da seakutni dijabetes javlja između osmog dana i tri nedelje, a hronični 3 nedeljenakon tretmana streptozotocinom. Ţivotinje sa dijabetesom su ili bilenetretirane 2 nedelje ili podvrgnute subhroničnom tretmanu insulinom (3 IU) utrajanju od 7 dana. Za analizu su selektirani signalni molekuli Akt i ERK kojipredstavljaju medijatore gotovo svih efekata insulina u srcu. Osim togaanalizirani su i efektorni molekuli regulisani preko aktivacije signalnih putevaAkt i ERK, kao što su eNOS i iNOS, uključeni u sintezu azot-monoksida, kao itransporteri glukoze GLUT1 i GLUT4 i transporter masnih kiselina CD36.Ispitivana je ekspresija ovih molekula na nivou gena i proteina i/ili fosforilacija,kao i unutarćelijska lokalizacija transportera za glukozu i masne kiseline u srcu.Poznato je da je kompeticija između arginaze i NOS za zajedničkisupstrat L-arginin, ograničavajući faktor za proizvodnju azot-monoksida...
AB  - nsulin deficiency and hyperglycemia, which are the main features ofType 1 diabetes are associated with a number of endocrine and metabolicdisorders and one of the most common complications is the increased incidenceof cardiovascular diseases. Therefore, appropriate insulin replacement therapyis necessary in order to control the disease, as well as for the prevention ofpotential complications.We hypothesized that insulin deficiency and hyperglycemia may lead tochanges in cardiac insulin signalling pathway which consequently may result inchanges in nitric oxide production and utilization of energy substrates, and thatinsulin replacement is required to correct the changes.To induce experimental Type 1 diabetes, male Wistar rats were injectedintraperitoneally with streptozotocin (65 mg/kg). Acute diabetes was reportedto occur between 8 days and 3 weeks, and chronic diabetes within 3 weeks afterstreptozotocin administration. Diabetic animals were either maintaineduntreated, or treated with insulin (3 IU) daily for a week. For analysis,intracellular signaling molecules Akt and ERK1/2 were selected, which areimportant intermediary of almost all effects of insulin in the heart. Furthermorewe analyzed effector molecules which are regulated through activation of Aktand ERK signaling pathways, such as eNOS and iNOS, involved in thesynthesis of nitric oxide, as well as glucose transporter GLUT1 and GLUT4 andtransporter of fatty acids CD36. In the present study we examined theexpression and/or phosphorylation of the molecules, as well as the subcellularlocalization of the transporters for glucose and fatty acid in the heart.It is known that the competition between arginase and NOS for acommon substrate L-arginine is a limiting factor for the production of nitricoxide...
PB  - Универзитет у Београду, Биолошки факултет
T2  - Универзитет у Београду
T1  - Efekat eksperimentalnog dijabetesa tipa 1 i supstitucione terapije na molekule regulisane insulinom u srcu pacova
T1  - The effect of experimentally induced Type 1 diabetes and insulin replacement therapy on insulin signalling pathways in the rat heart
ER  - 
@phdthesis{
author = "Stojiljković, Mojca D.",
year = "2014",
url = "http://eteze.bg.ac.rs/application/showtheses?thesesId=3811, https://fedorabg.bg.ac.rs/fedora/get/o:12801/bdef:Content/download, http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024763058, http://nardus.mpn.gov.rs/123456789/6495, http://vinar.vin.bg.ac.rs/handle/123456789/7302",
abstract = "Insulinska deficijencija i hiperglikemija, koje predstavljaju osnovnekarakteristike dijabetesa tipa 1, povezane su sa brojnim endokrinim imetaboličkim promenama, a jedna od najčešćih komplikacija je povećanaučestalost nastanka kardiovaskularnih oboljenja. Zbog toga je rana i adekvatnaprimena terapije insulinom neophodna, kako za kontrolu bolesti, tako i zaprevenciju potencijalnih komplikacija.Polazna hipoteza istraţivanja čiji su rezultati obuhvaćeni ovomdoktorskom disertacijom je bila da u stanju hipoinsulinemije i hiperglikemijemoţe doći do promena u signalnom putu insulina u srcu, što moţe da se odrazina produkciju azot-monoksida i iskorišćavanje energetskih supstrata, a da bisupstitucija insulinom trebala da koriguje nastale promene.Za uspostavljanje eksperimentalnog modela dijabetesa tipa 1, muţjacipacova soja Wistar tretirani su streptozotocinom (60 mg/kg). Poznato je da seakutni dijabetes javlja između osmog dana i tri nedelje, a hronični 3 nedeljenakon tretmana streptozotocinom. Ţivotinje sa dijabetesom su ili bilenetretirane 2 nedelje ili podvrgnute subhroničnom tretmanu insulinom (3 IU) utrajanju od 7 dana. Za analizu su selektirani signalni molekuli Akt i ERK kojipredstavljaju medijatore gotovo svih efekata insulina u srcu. Osim togaanalizirani su i efektorni molekuli regulisani preko aktivacije signalnih putevaAkt i ERK, kao što su eNOS i iNOS, uključeni u sintezu azot-monoksida, kao itransporteri glukoze GLUT1 i GLUT4 i transporter masnih kiselina CD36.Ispitivana je ekspresija ovih molekula na nivou gena i proteina i/ili fosforilacija,kao i unutarćelijska lokalizacija transportera za glukozu i masne kiseline u srcu.Poznato je da je kompeticija između arginaze i NOS za zajedničkisupstrat L-arginin, ograničavajući faktor za proizvodnju azot-monoksida..., nsulin deficiency and hyperglycemia, which are the main features ofType 1 diabetes are associated with a number of endocrine and metabolicdisorders and one of the most common complications is the increased incidenceof cardiovascular diseases. Therefore, appropriate insulin replacement therapyis necessary in order to control the disease, as well as for the prevention ofpotential complications.We hypothesized that insulin deficiency and hyperglycemia may lead tochanges in cardiac insulin signalling pathway which consequently may result inchanges in nitric oxide production and utilization of energy substrates, and thatinsulin replacement is required to correct the changes.To induce experimental Type 1 diabetes, male Wistar rats were injectedintraperitoneally with streptozotocin (65 mg/kg). Acute diabetes was reportedto occur between 8 days and 3 weeks, and chronic diabetes within 3 weeks afterstreptozotocin administration. Diabetic animals were either maintaineduntreated, or treated with insulin (3 IU) daily for a week. For analysis,intracellular signaling molecules Akt and ERK1/2 were selected, which areimportant intermediary of almost all effects of insulin in the heart. Furthermorewe analyzed effector molecules which are regulated through activation of Aktand ERK signaling pathways, such as eNOS and iNOS, involved in thesynthesis of nitric oxide, as well as glucose transporter GLUT1 and GLUT4 andtransporter of fatty acids CD36. In the present study we examined theexpression and/or phosphorylation of the molecules, as well as the subcellularlocalization of the transporters for glucose and fatty acid in the heart.It is known that the competition between arginase and NOS for acommon substrate L-arginine is a limiting factor for the production of nitricoxide...",
publisher = "Универзитет у Београду, Биолошки факултет",
journal = "Универзитет у Београду",
title = "Efekat eksperimentalnog dijabetesa tipa 1 i supstitucione terapije na molekule regulisane insulinom u srcu pacova, The effect of experimentally induced Type 1 diabetes and insulin replacement therapy on insulin signalling pathways in the rat heart"
}
Stojiljković, M. D. (2014). The effect of experimentally induced Type 1 diabetes and insulin replacement therapy on insulin signalling pathways in the rat heart.
Универзитет у Београду
Универзитет у Београду, Биолошки факултет..
Stojiljković MD. The effect of experimentally induced Type 1 diabetes and insulin replacement therapy on insulin signalling pathways in the rat heart. Универзитет у Београду. 2014;
Stojiljković Mojca D., "The effect of experimentally induced Type 1 diabetes and insulin replacement therapy on insulin signalling pathways in the rat heart" Универзитет у Београду (2014)