TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Spasojević, Nataša
AU  - Dronjak, Slađana
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4746
AB  - The sympatho-adrenal system represents one of the main systems involved in the response to stressful events because its stress-induced activation results in an increased release of catecholamines. Exercise training acts as an important modulator of sympatho-adrenal system, adrenal medulla and stellate ganglia being two components of this system. This study aimed at investigating physical exercise-related changes in gene expression of catecholamine biosynthetic enzymes tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase in the adrenal medulla and stellate ganglia of chronically psychosocially stressed adult rats exposed daily to 20-min treadmill exercise for 12 weeks, using TaqMan RT-PCR assay. Chronic psychosocial stress decreased gene expression of the examined enzymes in the adrenal medulla and treadmill exercise did not lead to further modulation of the corresponding gene expression. On the other hand, chronic psychosocial stress produced a significant increase of TH (about 51%) and DBH (about 103%) gene expression in stellate ganglia, while treadmill exercise decreased gene expression of these enzymes to control levels in psychosocially stressed rats. Our data indicate that treadmill exercise leads to a decreased gene transcription of catecholamine biosynthetic enzymes in stellate ganglia and attenuation of cardiac noradrenaline production in stressful situations. Reduction of catecholamine synthesis in stellate ganglia may be linked to the beneficial effects of treadmill exercise on cardiovascular system in stressed animals.
T2  - European Journal of Applied Physiology
T1  - Modulation of catecholamine-synthesizing enzymes in adrenal medulla and stellate ganglia by treadmill exercise of stressed rats
VL  - 112
IS  - 3
SP  - 1177
EP  - 1182
DO  - 10.1007/s00421-011-2046-5
ER  - 

@article{
author = "Gavrilović, Ljubica and Spasojević, Nataša and Dronjak, Slađana",
year = "2012",
abstract = "The sympatho-adrenal system represents one of the main systems involved in the response to stressful events because its stress-induced activation results in an increased release of catecholamines. Exercise training acts as an important modulator of sympatho-adrenal system, adrenal medulla and stellate ganglia being two components of this system. This study aimed at investigating physical exercise-related changes in gene expression of catecholamine biosynthetic enzymes tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase in the adrenal medulla and stellate ganglia of chronically psychosocially stressed adult rats exposed daily to 20-min treadmill exercise for 12 weeks, using TaqMan RT-PCR assay. Chronic psychosocial stress decreased gene expression of the examined enzymes in the adrenal medulla and treadmill exercise did not lead to further modulation of the corresponding gene expression. On the other hand, chronic psychosocial stress produced a significant increase of TH (about 51%) and DBH (about 103%) gene expression in stellate ganglia, while treadmill exercise decreased gene expression of these enzymes to control levels in psychosocially stressed rats. Our data indicate that treadmill exercise leads to a decreased gene transcription of catecholamine biosynthetic enzymes in stellate ganglia and attenuation of cardiac noradrenaline production in stressful situations. Reduction of catecholamine synthesis in stellate ganglia may be linked to the beneficial effects of treadmill exercise on cardiovascular system in stressed animals.",
journal = "European Journal of Applied Physiology",
title = "Modulation of catecholamine-synthesizing enzymes in adrenal medulla and stellate ganglia by treadmill exercise of stressed rats",
volume = "112",
number = "3",
pages = "1177-1182",
doi = "10.1007/s00421-011-2046-5"
}

Gavrilović, L., Spasojević, N.,& Dronjak, S.. (2012). Modulation of catecholamine-synthesizing enzymes in adrenal medulla and stellate ganglia by treadmill exercise of stressed rats. in European Journal of Applied Physiology, 112(3), 1177-1182.
https://doi.org/10.1007/s00421-011-2046-5

Gavrilović L, Spasojević N, Dronjak S. Modulation of catecholamine-synthesizing enzymes in adrenal medulla and stellate ganglia by treadmill exercise of stressed rats. in European Journal of Applied Physiology. 2012;112(3):1177-1182.
doi:10.1007/s00421-011-2046-5 .

Gavrilović, Ljubica, Spasojević, Nataša, Dronjak, Slađana, "Modulation of catecholamine-synthesizing enzymes in adrenal medulla and stellate ganglia by treadmill exercise of stressed rats" in European Journal of Applied Physiology, 112, no. 3 (2012):1177-1182,
https://doi.org/10.1007/s00421-011-2046-5 . .

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Keta, Otilija D.
AU  - Iannolo, Gioacchin
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6905
AB  - Response of human HTB140 melanoma cells to proton irradiation in combination with fotemustine (FM) was investigated. Effects of these agents were analysed on cell proliferation and induction of apoptosis. Cells pretreated with 100- or 250-mu M of FM were irradiated in the middle of the therapeutic 62-MeV proton spread-out Bragg peak, with a dose of 16 Gy. All treatments reduced proliferation and survival of melanoma cells. The most pronounced effects of the combined treatment were obtained for cell survivals. The level of apoptosis increased after all applied treatments. Particularly good pro-apoptotic effect was achieved when proton irradiation was combined with 250 mu M of FM. This was followed by the increased expression of p53 gene. The obtained results have shown that combined application of FM and protons significantly reduced growth of this resistant melanoma cell line.
T2  - Radiation Protection Dosimetry
T1  - Proton inactivation of melanoma cells enhanced by fotemustine
VL  - 143
IS  - 2-4
SP  - 503
EP  - 507
DO  - 10.1093/rpd/ncq527
ER  - 

@article{
author = "Ristić-Fira, Aleksandra and Korićanac, Lela and Žakula, Jelena and Keta, Otilija D. and Iannolo, Gioacchin and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2011",
abstract = "Response of human HTB140 melanoma cells to proton irradiation in combination with fotemustine (FM) was investigated. Effects of these agents were analysed on cell proliferation and induction of apoptosis. Cells pretreated with 100- or 250-mu M of FM were irradiated in the middle of the therapeutic 62-MeV proton spread-out Bragg peak, with a dose of 16 Gy. All treatments reduced proliferation and survival of melanoma cells. The most pronounced effects of the combined treatment were obtained for cell survivals. The level of apoptosis increased after all applied treatments. Particularly good pro-apoptotic effect was achieved when proton irradiation was combined with 250 mu M of FM. This was followed by the increased expression of p53 gene. The obtained results have shown that combined application of FM and protons significantly reduced growth of this resistant melanoma cell line.",
journal = "Radiation Protection Dosimetry",
title = "Proton inactivation of melanoma cells enhanced by fotemustine",
volume = "143",
number = "2-4",
pages = "503-507",
doi = "10.1093/rpd/ncq527"
}

Ristić-Fira, A., Korićanac, L., Žakula, J., Keta, O. D., Iannolo, G., Cuttone, G.,& Petrović, I. M.. (2011). Proton inactivation of melanoma cells enhanced by fotemustine. in Radiation Protection Dosimetry, 143(2-4), 503-507.
https://doi.org/10.1093/rpd/ncq527

Ristić-Fira A, Korićanac L, Žakula J, Keta OD, Iannolo G, Cuttone G, Petrović IM. Proton inactivation of melanoma cells enhanced by fotemustine. in Radiation Protection Dosimetry. 2011;143(2-4):503-507.
doi:10.1093/rpd/ncq527 .

Ristić-Fira, Aleksandra, Korićanac, Lela, Žakula, Jelena, Keta, Otilija D., Iannolo, Gioacchin, Cuttone, Giacomo, Petrović, Ivan M., "Proton inactivation of melanoma cells enhanced by fotemustine" in Radiation Protection Dosimetry, 143, no. 2-4 (2011):503-507,
https://doi.org/10.1093/rpd/ncq527 . .

TY  - JOUR
AU  - Spasojević, Nataša
AU  - Gavrilović, Ljubica
AU  - Dronjak, Slađana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4539
AB  - Stress exposure activates the sympathoneural system, resulting in catecholamine release. Chronic stress is associated with development of numerous disorders, including cardiovascular diseases. Here we investigated the expression of mRNAs for catecholamine biosynthetic enzymes tyrosine-hydroxylase, dopamine-beta-hydroxylase and phenylethanolamine N-methyltransferase, and for beta(1)- and beta(2)-adrenoceptors in the right and left ventricles of rats exposed to chronic unpredictable mild stress. The tyrosine-hydroxylase and dopamine-beta-hydroxylase mRNA levels were not affected by stress, whereas the phenylethanolamine N-methyltransferase mRNA levels significantly increased in both right and left ventricles. No changes in beta(1)-adrenoceptor mRNA levels in either right or left ventricles were observed. At the same time, stress produced a significant increase of beta(2)-adrenoceptor mRNA levels in left ventricles. These results suggest that elevated expression of phenylethanolamine N-methyltransferase in both ventricules and beta(2)-adrenoceptor genes in left ventricles could provide a molecular mechanism that leads to altered physiological response, which is important for the organism coping with stress.
T2  - Physiological Research
T1  - Regulation of Catecholamine-Synthesising Enzymes and beta-Adrenoceptors Gene Expression in Ventricles of Stressed Rats
VL  - 60
SP  - S171
EP  - S176
UR  - https://hdl.handle.net/21.15107/rcub_vinar_4539
ER  - 

@article{
author = "Spasojević, Nataša and Gavrilović, Ljubica and Dronjak, Slađana",
year = "2011",
abstract = "Stress exposure activates the sympathoneural system, resulting in catecholamine release. Chronic stress is associated with development of numerous disorders, including cardiovascular diseases. Here we investigated the expression of mRNAs for catecholamine biosynthetic enzymes tyrosine-hydroxylase, dopamine-beta-hydroxylase and phenylethanolamine N-methyltransferase, and for beta(1)- and beta(2)-adrenoceptors in the right and left ventricles of rats exposed to chronic unpredictable mild stress. The tyrosine-hydroxylase and dopamine-beta-hydroxylase mRNA levels were not affected by stress, whereas the phenylethanolamine N-methyltransferase mRNA levels significantly increased in both right and left ventricles. No changes in beta(1)-adrenoceptor mRNA levels in either right or left ventricles were observed. At the same time, stress produced a significant increase of beta(2)-adrenoceptor mRNA levels in left ventricles. These results suggest that elevated expression of phenylethanolamine N-methyltransferase in both ventricules and beta(2)-adrenoceptor genes in left ventricles could provide a molecular mechanism that leads to altered physiological response, which is important for the organism coping with stress.",
journal = "Physiological Research",
title = "Regulation of Catecholamine-Synthesising Enzymes and beta-Adrenoceptors Gene Expression in Ventricles of Stressed Rats",
volume = "60",
pages = "S171-S176",
url = "https://hdl.handle.net/21.15107/rcub_vinar_4539"
}

Spasojević, N., Gavrilović, L.,& Dronjak, S.. (2011). Regulation of Catecholamine-Synthesising Enzymes and beta-Adrenoceptors Gene Expression in Ventricles of Stressed Rats. in Physiological Research, 60, S171-S176.
https://hdl.handle.net/21.15107/rcub_vinar_4539

Spasojević N, Gavrilović L, Dronjak S. Regulation of Catecholamine-Synthesising Enzymes and beta-Adrenoceptors Gene Expression in Ventricles of Stressed Rats. in Physiological Research. 2011;60:S171-S176.
https://hdl.handle.net/21.15107/rcub_vinar_4539 .

Spasojević, Nataša, Gavrilović, Ljubica, Dronjak, Slađana, "Regulation of Catecholamine-Synthesising Enzymes and beta-Adrenoceptors Gene Expression in Ventricles of Stressed Rats" in Physiological Research, 60 (2011):S171-S176,
https://hdl.handle.net/21.15107/rcub_vinar_4539 .

TY  - JOUR
AU  - Horvat, Anica
AU  - Stanojević, Ivana
AU  - Drakulić, Dunja R.
AU  - Velickovic, Natasa
AU  - Petrović, Snježana
AU  - Milošević, Maja
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3941
AB  - The aim of the present study was to examine the effect of acute restraint stress on rat brain synaptosomal plasma membrane (SPM) ecto-nucleotidase activities at specific stages of postnatal development (15-, 30-, 60- and 90-day-old rats) by measuring the rates of ATP, ADP and AMP hydrolysis 1,24 and 72 h post-stress. At 1 h after stress NTPDase and ecto-5-nucleotidase activities were decreased in rats aged up to 60 days old. In adult rats elevated enzyme activities were detected, which indicated the existence of different short-term stress responses during development. A similar pattern of ATP and ADP hydrolysis changes as well as the ATP/ADP ratio in all developmental stages indicated that NTPDase3 was acutely affected after stress. The long-term effect of acute stress on NTPDase activity differed during postnatal development. In juvenile animals (15 days old) NTPDase activity was not altered. However, in later developmental stages (30 and 60 days old rats) NTPDase activity decreased and persisted for 72 h post-stress. In adult rats only ATP hydrolysis was decreased after 24 h, indicating that ecto-ATPase was affected by stress. Ecto-5-nucleotidase hydrolysing activity was decreased within 24 h in adult rats, while in 15- and 30-day old rats it decreased 72 h post-stress. At equivalent times in pubertal rats (60 days old) a slight activation of ecto-5-nucleotidase was detected. Our results highlight the developmental-dependence of brain ecto-nucleotidase susceptibility to acute stress and the likely existence of different mechanisms involved in time-dependent ecto-nucleotidase activity modulation following stress exposure. Clearly there are differences in the response of the purinergic system to acute restraint stress between young and adult rats. (C) 2009 ISDN. Published by Elsevier Ltd. All rights reserved.
T2  - International Journal of Developmental Neuroscience
T1  - Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development
VL  - 28
IS  - 2
SP  - 175
EP  - 182
DO  - 10.1016/j.ijdevneu.2009.11.005
ER  - 

@article{
author = "Horvat, Anica and Stanojević, Ivana and Drakulić, Dunja R. and Velickovic, Natasa and Petrović, Snježana and Milošević, Maja",
year = "2010",
abstract = "The aim of the present study was to examine the effect of acute restraint stress on rat brain synaptosomal plasma membrane (SPM) ecto-nucleotidase activities at specific stages of postnatal development (15-, 30-, 60- and 90-day-old rats) by measuring the rates of ATP, ADP and AMP hydrolysis 1,24 and 72 h post-stress. At 1 h after stress NTPDase and ecto-5-nucleotidase activities were decreased in rats aged up to 60 days old. In adult rats elevated enzyme activities were detected, which indicated the existence of different short-term stress responses during development. A similar pattern of ATP and ADP hydrolysis changes as well as the ATP/ADP ratio in all developmental stages indicated that NTPDase3 was acutely affected after stress. The long-term effect of acute stress on NTPDase activity differed during postnatal development. In juvenile animals (15 days old) NTPDase activity was not altered. However, in later developmental stages (30 and 60 days old rats) NTPDase activity decreased and persisted for 72 h post-stress. In adult rats only ATP hydrolysis was decreased after 24 h, indicating that ecto-ATPase was affected by stress. Ecto-5-nucleotidase hydrolysing activity was decreased within 24 h in adult rats, while in 15- and 30-day old rats it decreased 72 h post-stress. At equivalent times in pubertal rats (60 days old) a slight activation of ecto-5-nucleotidase was detected. Our results highlight the developmental-dependence of brain ecto-nucleotidase susceptibility to acute stress and the likely existence of different mechanisms involved in time-dependent ecto-nucleotidase activity modulation following stress exposure. Clearly there are differences in the response of the purinergic system to acute restraint stress between young and adult rats. (C) 2009 ISDN. Published by Elsevier Ltd. All rights reserved.",
journal = "International Journal of Developmental Neuroscience",
title = "Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development",
volume = "28",
number = "2",
pages = "175-182",
doi = "10.1016/j.ijdevneu.2009.11.005"
}

Horvat, A., Stanojević, I., Drakulić, D. R., Velickovic, N., Petrović, S.,& Milošević, M.. (2010). Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development. in International Journal of Developmental Neuroscience, 28(2), 175-182.
https://doi.org/10.1016/j.ijdevneu.2009.11.005

Horvat A, Stanojević I, Drakulić DR, Velickovic N, Petrović S, Milošević M. Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development. in International Journal of Developmental Neuroscience. 2010;28(2):175-182.
doi:10.1016/j.ijdevneu.2009.11.005 .

Horvat, Anica, Stanojević, Ivana, Drakulić, Dunja R., Velickovic, Natasa, Petrović, Snježana, Milošević, Maja, "Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development" in International Journal of Developmental Neuroscience, 28, no. 2 (2010):175-182,
https://doi.org/10.1016/j.ijdevneu.2009.11.005 . .

TY  - CONF
AU  - Milošević, Maja
AU  - Drakulić, Dunja R.
AU  - Petrović, Snježana
AU  - Stanojević, Ivana
AU  - Veličković, Nataša
AU  - Horvat, Anica
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9319
AB  - L-cysteine is used as effective oral chelating agent due to property of its sulfhydryl
group to bind heavy metal ions. The aim of this study was to investigate ability of
L-cysteine to prevent mercury (II) and cadmium (II) induced ecto-ATPase activity
inhibition of rat uterus plasma membranes. Results show that 10 mmol/l L-cysteine
have protective effect on enzyme activity in the presence of cadmium and mercury
ions.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9319
ER  - 

@conference{
author = "Milošević, Maja and Drakulić, Dunja R. and Petrović, Snježana and Stanojević, Ivana and Veličković, Nataša and Horvat, Anica",
year = "2010",
abstract = "L-cysteine is used as effective oral chelating agent due to property of its sulfhydryl
group to bind heavy metal ions. The aim of this study was to investigate ability of
L-cysteine to prevent mercury (II) and cadmium (II) induced ecto-ATPase activity
inhibition of rat uterus plasma membranes. Results show that 10 mmol/l L-cysteine
have protective effect on enzyme activity in the presence of cadmium and mercury
ions.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9319"
}

Milošević, M., Drakulić, D. R., Petrović, S., Stanojević, I., Veličković, N.,& Horvat, A.. (2010). L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
https://hdl.handle.net/21.15107/rcub_vinar_9319

Milošević M, Drakulić DR, Petrović S, Stanojević I, Veličković N, Horvat A. L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;.
https://hdl.handle.net/21.15107/rcub_vinar_9319 .

Milošević, Maja, Drakulić, Dunja R., Petrović, Snježana, Stanojević, Ivana, Veličković, Nataša, Horvat, Anica, "L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010),
https://hdl.handle.net/21.15107/rcub_vinar_9319 .

TY  - CONF
AU  - Petrović, Snježana
AU  - Milošević, Maja
AU  - Stanojević, Ivana
AU  - Drakulić, Dunja R.
AU  - Jovanović, N.
AU  - Veličković, Nataša
AU  - Horvat, Anica
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9321
AB  - In this study the Ca2+ ion flux modulation in the synaptosomal mitochondria
isolated from caudate nucleus (CN) of the ovariectomised rats was examined.
17 beta-estradiol (E2), E2-conjugated to bovine serum albumin (E-BSA), estradiol
receptor α (ERα) agonist 4,4',4''-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol
(PPT), ERβ agonist 2,3-bis(4-hydroxyphenyl)-propionitrile (DNP) and ERα/β
antagonist 7α,17β-[9[(4,4,5,5,5-pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-
triene-3,17-diol (ICI 182,780) were used. The Ca2+ efflux inhibition of about 27%
was detected in the presence of 0.5 nmol/l E2, and of about 20% in the case of
E-BSA. DNP (10 nmol/l) was as much potent Ca2+ efflux inhibitor as E2, while
PPT (10 nmol/l) hardly had any inhibitory effect (9% efflux decrease). When E2
binding to ERα and ERβ was prevented by 1 μmol/l ICI 182,780, the Ca2+ efflux
inhibition of about 15% was detected. Our results suggest that E2 prevents Ca2+
efflux from synaptosomal mitochondria due to ERβ activation rather than ERα.
The involvement of the external E2 binding site on the mitochondrial membrane
probably different from ERα/β should not be excluded because of Ca2+ efflux
inhibition detected in the presence of E-BSA and ICI 182,780. The Ca2+ efflux
modulation could be the mechanism through which E2 exerts its neuromodulatory
role in specific brain structures.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9321
ER  - 

@conference{
author = "Petrović, Snježana and Milošević, Maja and Stanojević, Ivana and Drakulić, Dunja R. and Jovanović, N. and Veličković, Nataša and Horvat, Anica",
year = "2010",
abstract = "In this study the Ca2+ ion flux modulation in the synaptosomal mitochondria
isolated from caudate nucleus (CN) of the ovariectomised rats was examined.
17 beta-estradiol (E2), E2-conjugated to bovine serum albumin (E-BSA), estradiol
receptor α (ERα) agonist 4,4',4''-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol
(PPT), ERβ agonist 2,3-bis(4-hydroxyphenyl)-propionitrile (DNP) and ERα/β
antagonist 7α,17β-[9[(4,4,5,5,5-pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-
triene-3,17-diol (ICI 182,780) were used. The Ca2+ efflux inhibition of about 27%
was detected in the presence of 0.5 nmol/l E2, and of about 20% in the case of
E-BSA. DNP (10 nmol/l) was as much potent Ca2+ efflux inhibitor as E2, while
PPT (10 nmol/l) hardly had any inhibitory effect (9% efflux decrease). When E2
binding to ERα and ERβ was prevented by 1 μmol/l ICI 182,780, the Ca2+ efflux
inhibition of about 15% was detected. Our results suggest that E2 prevents Ca2+
efflux from synaptosomal mitochondria due to ERβ activation rather than ERα.
The involvement of the external E2 binding site on the mitochondrial membrane
probably different from ERα/β should not be excluded because of Ca2+ efflux
inhibition detected in the presence of E-BSA and ICI 182,780. The Ca2+ efflux
modulation could be the mechanism through which E2 exerts its neuromodulatory
role in specific brain structures.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9321"
}

Petrović, S., Milošević, M., Stanojević, I., Drakulić, D. R., Jovanović, N., Veličković, N.,& Horvat, A.. (2010). Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
https://hdl.handle.net/21.15107/rcub_vinar_9321

Petrović S, Milošević M, Stanojević I, Drakulić DR, Jovanović N, Veličković N, Horvat A. Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;.
https://hdl.handle.net/21.15107/rcub_vinar_9321 .

Petrović, Snježana, Milošević, Maja, Stanojević, Ivana, Drakulić, Dunja R., Jovanović, N., Veličković, Nataša, Horvat, Anica, "Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010),
https://hdl.handle.net/21.15107/rcub_vinar_9321 .

TY  - CONF
AU  - Stanojević, Ivana
AU  - Milošević, Maja
AU  - Petrović, Snježana
AU  - Drakulić, Dunja R.
AU  - Veličković, Nataša
AU  - Horvat, Anica
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9324
AB  - Kinetic properties of ecto-nucleoside triphosphate diphosphohydrolase (NTPDases)
in synaptic terminals from whole rat brain were characterised. Three different
NTPDases are present in nervous system and hydrolysing ATP and ADP with
different affinities. ATP and ADP hydrolysis exhibited kinetic properties typical
for all members of the NTPDase, low substrate specificity for tri- and
diphosphonucleosides, divalent cation dependency and insensitivity towards iontransporting
ATPase inhibitors. Kinetic properties of ATPase and ADPase
component of NTPDases depend on postnatal developmental stages of rats, and
indicated different NTPDase family members present in various ontogeny stages.
Lowering in the NTPDases activities could result in a decreased production of
adenosine and change in extracellular ATP/adenosine ratio.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Kinetic properties of ntpdases in rat brain synaptic plasma membranes
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9324
ER  - 

@conference{
author = "Stanojević, Ivana and Milošević, Maja and Petrović, Snježana and Drakulić, Dunja R. and Veličković, Nataša and Horvat, Anica",
year = "2010",
abstract = "Kinetic properties of ecto-nucleoside triphosphate diphosphohydrolase (NTPDases)
in synaptic terminals from whole rat brain were characterised. Three different
NTPDases are present in nervous system and hydrolysing ATP and ADP with
different affinities. ATP and ADP hydrolysis exhibited kinetic properties typical
for all members of the NTPDase, low substrate specificity for tri- and
diphosphonucleosides, divalent cation dependency and insensitivity towards iontransporting
ATPase inhibitors. Kinetic properties of ATPase and ADPase
component of NTPDases depend on postnatal developmental stages of rats, and
indicated different NTPDase family members present in various ontogeny stages.
Lowering in the NTPDases activities could result in a decreased production of
adenosine and change in extracellular ATP/adenosine ratio.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Kinetic properties of ntpdases in rat brain synaptic plasma membranes",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9324"
}

Stanojević, I., Milošević, M., Petrović, S., Drakulić, D. R., Veličković, N.,& Horvat, A.. (2010). Kinetic properties of ntpdases in rat brain synaptic plasma membranes. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
https://hdl.handle.net/21.15107/rcub_vinar_9324

Stanojević I, Milošević M, Petrović S, Drakulić DR, Veličković N, Horvat A. Kinetic properties of ntpdases in rat brain synaptic plasma membranes. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;.
https://hdl.handle.net/21.15107/rcub_vinar_9324 .

Stanojević, Ivana, Milošević, Maja, Petrović, Snježana, Drakulić, Dunja R., Veličković, Nataša, Horvat, Anica, "Kinetic properties of ntpdases in rat brain synaptic plasma membranes" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010),
https://hdl.handle.net/21.15107/rcub_vinar_9324 .

TY  - JOUR
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Keta, Otilija D.
AU  - Privitera G.
AU  - Cirrone, G. A. P.
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10506
AB  - Deregulation of apoptosis commonly occurs in melanoma cells and could be a reason for resistance. The effectiveness of different treatments depends on their ability to activate this process. In this study the effects of combined treatments with fotemustine (FM) and proton irradiation on the regulators of apoptosis were analyzed. Sub-confluent HTB140 human melanoma cells were treated with FM (100, 250 µM) 24 h prior to irradiation (12, 16 Gy). Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak. Flow cytometric analysis of apoptosis and the Western blot analysis of apoptotic regulators were performed 6 or 48 h after treatments. Percent of apoptotic nuclei increased after applied treatments, reaching the level of 4 to 41 %. Induction of apoptosis was associated with p53 and Bax up regulation and Bcl-2 down regulation. The obtained results imply that analyzed treatments induce apoptosis through the activation of the mitochondrial apoptotic pathway, with better pro-apoptotic effects achieved by combined treatments.
T2  - INFN-LNS Activity report 2009
T1  - Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation
SP  - 234
EP  - 237
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10506
ER  - 

@article{
author = "Korićanac, Lela and Žakula, Jelena and Keta, Otilija D. and Privitera G. and Cirrone, G. A. P. and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2010",
abstract = "Deregulation of apoptosis commonly occurs in melanoma cells and could be a reason for resistance. The effectiveness of different treatments depends on their ability to activate this process. In this study the effects of combined treatments with fotemustine (FM) and proton irradiation on the regulators of apoptosis were analyzed. Sub-confluent HTB140 human melanoma cells were treated with FM (100, 250 µM) 24 h prior to irradiation (12, 16 Gy). Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak. Flow cytometric analysis of apoptosis and the Western blot analysis of apoptotic regulators were performed 6 or 48 h after treatments. Percent of apoptotic nuclei increased after applied treatments, reaching the level of 4 to 41 %. Induction of apoptosis was associated with p53 and Bax up regulation and Bcl-2 down regulation. The obtained results imply that analyzed treatments induce apoptosis through the activation of the mitochondrial apoptotic pathway, with better pro-apoptotic effects achieved by combined treatments.",
journal = "INFN-LNS Activity report 2009",
title = "Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation",
pages = "234-237",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10506"
}

Korićanac, L., Žakula, J., Keta, O. D., Privitera G., Cirrone, G. A. P., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2010). Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation. in INFN-LNS Activity report 2009, 234-237.
https://hdl.handle.net/21.15107/rcub_vinar_10506

Korićanac L, Žakula J, Keta OD, Privitera G., Cirrone GAP, Cuttone G, Petrović IM, Ristić-Fira A. Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation. in INFN-LNS Activity report 2009. 2010;:234-237.
https://hdl.handle.net/21.15107/rcub_vinar_10506 .

Korićanac, Lela, Žakula, Jelena, Keta, Otilija D., Privitera G., Cirrone, G. A. P., Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation" in INFN-LNS Activity report 2009 (2010):234-237,
https://hdl.handle.net/21.15107/rcub_vinar_10506 .

TY  - JOUR
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Romano, F.
AU  - Cirrone, G. A. P.
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10625
AB  - Exposure to irradiation can trigger the p53 tumor suppressor to induce cell growth arrest or apoptosis. This study was conducted in order to evaluate the ability of carbon ions to induce apoptosis. The HTB140 melanoma cells were irradiated at three positions along the Bragg curve of the 62 MeV/u 12C ion beams. In this way the cells were exposed to different high linear energy transfer (LET) values. The percentage of apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic indexes were calculated. The expression of apoptosis-associated proteins, p53 and Bax was estimated by Western blot analyses. A dose dependent increase of apoptosis was noticed in all irradiation positions. When moving along the Bragg curve, i.e., with the raise of LET, the level of apoptosis increased, but was somewhat attenuated for the highest LET value. The corresponding apoptotic indexes ranged from 2.45 to 7.71. Moreover, the induction of apoptosis was associated with p53 and Bax up regulation
T2  - INFN-LNS Activity report 2009
T1  - Apoptosis of HTBL40 melanoma cells induced by carbon ions of different let
SP  - 238
EP  - 241
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10625
ER  - 

@article{
author = "Žakula, Jelena and Korićanac, Lela and Keta, Otilija D. and Romano, F. and Cirrone, G. A. P. and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2010",
abstract = "Exposure to irradiation can trigger the p53 tumor suppressor to induce cell growth arrest or apoptosis. This study was conducted in order to evaluate the ability of carbon ions to induce apoptosis. The HTB140 melanoma cells were irradiated at three positions along the Bragg curve of the 62 MeV/u 12C ion beams. In this way the cells were exposed to different high linear energy transfer (LET) values. The percentage of apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic indexes were calculated. The expression of apoptosis-associated proteins, p53 and Bax was estimated by Western blot analyses. A dose dependent increase of apoptosis was noticed in all irradiation positions. When moving along the Bragg curve, i.e., with the raise of LET, the level of apoptosis increased, but was somewhat attenuated for the highest LET value. The corresponding apoptotic indexes ranged from 2.45 to 7.71. Moreover, the induction of apoptosis was associated with p53 and Bax up regulation",
journal = "INFN-LNS Activity report 2009",
title = "Apoptosis of HTBL40 melanoma cells induced by carbon ions of different let",
pages = "238-241",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10625"
}

Žakula, J., Korićanac, L., Keta, O. D., Romano, F., Cirrone, G. A. P., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2010). Apoptosis of HTBL40 melanoma cells induced by carbon ions of different let. in INFN-LNS Activity report 2009, 238-241.
https://hdl.handle.net/21.15107/rcub_vinar_10625

Žakula J, Korićanac L, Keta OD, Romano F, Cirrone GAP, Cuttone G, Petrović IM, Ristić-Fira A. Apoptosis of HTBL40 melanoma cells induced by carbon ions of different let. in INFN-LNS Activity report 2009. 2010;:238-241.
https://hdl.handle.net/21.15107/rcub_vinar_10625 .

Žakula, Jelena, Korićanac, Lela, Keta, Otilija D., Romano, F., Cirrone, G. A. P., Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Apoptosis of HTBL40 melanoma cells induced by carbon ions of different let" in INFN-LNS Activity report 2009 (2010):238-241,
https://hdl.handle.net/21.15107/rcub_vinar_10625 .

TY  - JOUR
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Romano, F.
AU  - Cirrone, G. A. P.
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11379
AB  - Exposure to irradiation can trigger the p53 tumor suppressor to induce cell growth arrest or apoptosis. This study was conducted in order to evaluate the ability of carbon ions to induce apoptosis. The HTB140 melanoma cells were irradiated at three positions along the Bragg curve of the 62 MeV/u 12C ion beams. In this way the cells were exposed to different high linear energy transfer (LET) values. The percentage of apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic indexes were calculated. The expression of apoptosis-associated proteins, p53 and Bax was estimated by Western blot analyses. A dose dependent increase of apoptosis was noticed in all irradiation positions. When moving along the Bragg curve, i.e., with the raise of LET, the level of apoptosis increased, but was somewhat attenuated for the highest LET value. The corresponding apoptotic indexes ranged from 2.45 to 7.71. Moreover, the induction of apoptosis was associated with p53 and Bax up regulation.
T2  - INFN-LNS Activity report 2009
T1  - Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET
SP  - 238
EP  - 241
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11379
ER  - 

@article{
author = "Žakula, Jelena and Korićanac, Lela and Keta, Otilija D. and Romano, F. and Cirrone, G. A. P. and Cuttone, Giacomo and Ristić-Fira, Aleksandra and Petrović, Ivan M.",
year = "2010",
abstract = "Exposure to irradiation can trigger the p53 tumor suppressor to induce cell growth arrest or apoptosis. This study was conducted in order to evaluate the ability of carbon ions to induce apoptosis. The HTB140 melanoma cells were irradiated at three positions along the Bragg curve of the 62 MeV/u 12C ion beams. In this way the cells were exposed to different high linear energy transfer (LET) values. The percentage of apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic indexes were calculated. The expression of apoptosis-associated proteins, p53 and Bax was estimated by Western blot analyses. A dose dependent increase of apoptosis was noticed in all irradiation positions. When moving along the Bragg curve, i.e., with the raise of LET, the level of apoptosis increased, but was somewhat attenuated for the highest LET value. The corresponding apoptotic indexes ranged from 2.45 to 7.71. Moreover, the induction of apoptosis was associated with p53 and Bax up regulation.",
journal = "INFN-LNS Activity report 2009",
title = "Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET",
pages = "238-241",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11379"
}

Žakula, J., Korićanac, L., Keta, O. D., Romano, F., Cirrone, G. A. P., Cuttone, G., Ristić-Fira, A.,& Petrović, I. M.. (2010). Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET. in INFN-LNS Activity report 2009, 238-241.
https://hdl.handle.net/21.15107/rcub_vinar_11379

Žakula J, Korićanac L, Keta OD, Romano F, Cirrone GAP, Cuttone G, Ristić-Fira A, Petrović IM. Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET. in INFN-LNS Activity report 2009. 2010;:238-241.
https://hdl.handle.net/21.15107/rcub_vinar_11379 .

Žakula, Jelena, Korićanac, Lela, Keta, Otilija D., Romano, F., Cirrone, G. A. P., Cuttone, Giacomo, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET" in INFN-LNS Activity report 2009 (2010):238-241,
https://hdl.handle.net/21.15107/rcub_vinar_11379 .

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Pajović, Snežana B.
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4048
AB  - In the present study we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex, as well as the serum corticosterone and glucose level of mature male Wistar rats subjected to three different treatments: animals subjected to chronic restraint-stress, animals injected with lithium (Li) and chronically stressed rats treated with Li. Under all three conditions we observed hypertrophy of whole adrenals, as well as the adrenal cortices. Chronic restraint stress, solely or in combination with Li treatment, significantly elevated the corticosterone level, but did not change the blood glucose level. Animals treated only with Li exhibited an elevated serum corticosterone level and blood glucose level. The aim of our study was to investigate the modulation of the chronic stress-induced effect on the blood glucose level by lithium, as a possible mechanism of avoiding the damage caused by chronic stress. Our results showed that lithium is an agent of choice which may help to reduce stress-elevated corticosterone and replenish exhausted glucose storages in an organism.
T2  - Archives of Biological Sciences
T1  - Lithium Modulates the Chronic Stress-Induced Effect on Blood Glucose Level of Male Rats
VL  - 62
IS  - 2
SP  - 289
EP  - 295
DO  - 10.2298/ABS1002289P
ER  - 

@article{
author = "Popović, Nataša M. and Pajović, Snežana B.",
year = "2010",
abstract = "In the present study we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex, as well as the serum corticosterone and glucose level of mature male Wistar rats subjected to three different treatments: animals subjected to chronic restraint-stress, animals injected with lithium (Li) and chronically stressed rats treated with Li. Under all three conditions we observed hypertrophy of whole adrenals, as well as the adrenal cortices. Chronic restraint stress, solely or in combination with Li treatment, significantly elevated the corticosterone level, but did not change the blood glucose level. Animals treated only with Li exhibited an elevated serum corticosterone level and blood glucose level. The aim of our study was to investigate the modulation of the chronic stress-induced effect on the blood glucose level by lithium, as a possible mechanism of avoiding the damage caused by chronic stress. Our results showed that lithium is an agent of choice which may help to reduce stress-elevated corticosterone and replenish exhausted glucose storages in an organism.",
journal = "Archives of Biological Sciences",
title = "Lithium Modulates the Chronic Stress-Induced Effect on Blood Glucose Level of Male Rats",
volume = "62",
number = "2",
pages = "289-295",
doi = "10.2298/ABS1002289P"
}

Popović, N. M.,& Pajović, S. B.. (2010). Lithium Modulates the Chronic Stress-Induced Effect on Blood Glucose Level of Male Rats. in Archives of Biological Sciences, 62(2), 289-295.
https://doi.org/10.2298/ABS1002289P

Popović NM, Pajović SB. Lithium Modulates the Chronic Stress-Induced Effect on Blood Glucose Level of Male Rats. in Archives of Biological Sciences. 2010;62(2):289-295.
doi:10.2298/ABS1002289P .

Popović, Nataša M., Pajović, Snežana B., "Lithium Modulates the Chronic Stress-Induced Effect on Blood Glucose Level of Male Rats" in Archives of Biological Sciences, 62, no. 2 (2010):289-295,
https://doi.org/10.2298/ABS1002289P . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Spasojević, Nataša
AU  - Dronjak, Slađana
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3988
AB  - Since previous experience of stressful situation profoundly affects response to a subsequent novel stressor, we examined changes in gene expression and protein levels of catecholamine biosynthetic enzymes in cardiac ventricles after exposure of chronic psychosocially isolated adult Wistar male rats to short-term immobilization stress. Chronic social isolation did not affect gene expression of tyrosine hydroxylase (TH) in either right or left ventricle. Subsequent immonilization of these animals produced an elevation of TH mRNA level in right and left ventricles. The levels of dopamine-beta-hydroxylase (DBH) mRNA were detectable only after immobilization both in right and left ventricles of control and chronically isolated rats. Chronic isolation stress increased phenylethanolamine N-methyltransferase (PNMT) mRNA levels in the right ventricle. Immobilization led to an elevated PNMT mRNA level in right and left ventricles of both control and chronically stressed animals. Protein levels of TH, DBH, and PNMT in right and left ventricles of socially isolated rats were increased after subsequent immobilization. Taking into consideration the role of cardiac catecholamines in physiological and pathophysiological processes, it could be hypothesized that increased catecholamine synthesis in the ventricles after novel immobilization stress could point to the susceptibility of the heart to subsequent stress.
T2  - Endocrine
T1  - Subsequent stress increases gene expression of catecholamine synthetic enzymes in cardiac ventricles of chronic-stressed rats
VL  - 37
IS  - 3
SP  - 425
EP  - 429
DO  - 10.1007/s12020-010-9325-5
ER  - 

@article{
author = "Gavrilović, Ljubica and Spasojević, Nataša and Dronjak, Slađana",
year = "2010",
abstract = "Since previous experience of stressful situation profoundly affects response to a subsequent novel stressor, we examined changes in gene expression and protein levels of catecholamine biosynthetic enzymes in cardiac ventricles after exposure of chronic psychosocially isolated adult Wistar male rats to short-term immobilization stress. Chronic social isolation did not affect gene expression of tyrosine hydroxylase (TH) in either right or left ventricle. Subsequent immonilization of these animals produced an elevation of TH mRNA level in right and left ventricles. The levels of dopamine-beta-hydroxylase (DBH) mRNA were detectable only after immobilization both in right and left ventricles of control and chronically isolated rats. Chronic isolation stress increased phenylethanolamine N-methyltransferase (PNMT) mRNA levels in the right ventricle. Immobilization led to an elevated PNMT mRNA level in right and left ventricles of both control and chronically stressed animals. Protein levels of TH, DBH, and PNMT in right and left ventricles of socially isolated rats were increased after subsequent immobilization. Taking into consideration the role of cardiac catecholamines in physiological and pathophysiological processes, it could be hypothesized that increased catecholamine synthesis in the ventricles after novel immobilization stress could point to the susceptibility of the heart to subsequent stress.",
journal = "Endocrine",
title = "Subsequent stress increases gene expression of catecholamine synthetic enzymes in cardiac ventricles of chronic-stressed rats",
volume = "37",
number = "3",
pages = "425-429",
doi = "10.1007/s12020-010-9325-5"
}

Gavrilović, L., Spasojević, N.,& Dronjak, S.. (2010). Subsequent stress increases gene expression of catecholamine synthetic enzymes in cardiac ventricles of chronic-stressed rats. in Endocrine, 37(3), 425-429.
https://doi.org/10.1007/s12020-010-9325-5

Gavrilović L, Spasojević N, Dronjak S. Subsequent stress increases gene expression of catecholamine synthetic enzymes in cardiac ventricles of chronic-stressed rats. in Endocrine. 2010;37(3):425-429.
doi:10.1007/s12020-010-9325-5 .

Gavrilović, Ljubica, Spasojević, Nataša, Dronjak, Slađana, "Subsequent stress increases gene expression of catecholamine synthetic enzymes in cardiac ventricles of chronic-stressed rats" in Endocrine, 37, no. 3 (2010):425-429,
https://doi.org/10.1007/s12020-010-9325-5 . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Spasojević, Nataša
AU  - Varagic, V.
AU  - Dronjak, Slađana
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3962
AB  - Enhanced activation of sympathetic neurons during stress results in an increased cardiovascular function. Social isolation is a psychological stress which has deleterious effects on health and represents the most relevant cause of diseases in mammalian species. In this study we investigated the changes in catecholamine biosynthetic enzymes tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) gene expression and protein levels in the stellate ganglia of naive controls and chronically socially isolated (12 weeks) adult rats and the response of these animals to additional immobilization stress (2 h) by applying TaqMan RT-PCR assay and Western blot analysis. Psychosocial stress produced a significant increase of both TH mRNA (p LT 0.05) and DBH mRNA (p LT 0.05) levels in stellate ganglia. The exposure of control rats to acute immobilization significantly increased TH mRNA (p LT 0.001) and DBH mRNA (p LT 0.01) levels, while additional immobilization of chronic psychosocially stressed rats expressed no effect on gene expression of these enzymes. Protein levels of TH, and DBH remained unchanged in control and chronic social isolation rats and also after short-term immobilization. The results presented here suggest that psychosocial stress-induced an increase in gene expression of catecholamine biosynthetic enzymes in stellate ganglia and thus may be connected to the increased risk of cardiovascular disease.
T2  - Acta Veterinaria, Beograd
T1  - Gene Expression of Catecholamine Synthesizing Enzymes in Stellate Ganglia of Stressed Rats
VL  - 60
IS  - 1
SP  - 15
EP  - 22
DO  - 10.2298/AVB1001015G
ER  - 

@article{
author = "Gavrilović, Ljubica and Spasojević, Nataša and Varagic, V. and Dronjak, Slađana",
year = "2010",
abstract = "Enhanced activation of sympathetic neurons during stress results in an increased cardiovascular function. Social isolation is a psychological stress which has deleterious effects on health and represents the most relevant cause of diseases in mammalian species. In this study we investigated the changes in catecholamine biosynthetic enzymes tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) gene expression and protein levels in the stellate ganglia of naive controls and chronically socially isolated (12 weeks) adult rats and the response of these animals to additional immobilization stress (2 h) by applying TaqMan RT-PCR assay and Western blot analysis. Psychosocial stress produced a significant increase of both TH mRNA (p LT 0.05) and DBH mRNA (p LT 0.05) levels in stellate ganglia. The exposure of control rats to acute immobilization significantly increased TH mRNA (p LT 0.001) and DBH mRNA (p LT 0.01) levels, while additional immobilization of chronic psychosocially stressed rats expressed no effect on gene expression of these enzymes. Protein levels of TH, and DBH remained unchanged in control and chronic social isolation rats and also after short-term immobilization. The results presented here suggest that psychosocial stress-induced an increase in gene expression of catecholamine biosynthetic enzymes in stellate ganglia and thus may be connected to the increased risk of cardiovascular disease.",
journal = "Acta Veterinaria, Beograd",
title = "Gene Expression of Catecholamine Synthesizing Enzymes in Stellate Ganglia of Stressed Rats",
volume = "60",
number = "1",
pages = "15-22",
doi = "10.2298/AVB1001015G"
}

Gavrilović, L., Spasojević, N., Varagic, V.,& Dronjak, S.. (2010). Gene Expression of Catecholamine Synthesizing Enzymes in Stellate Ganglia of Stressed Rats. in Acta Veterinaria, Beograd, 60(1), 15-22.
https://doi.org/10.2298/AVB1001015G

Gavrilović L, Spasojević N, Varagic V, Dronjak S. Gene Expression of Catecholamine Synthesizing Enzymes in Stellate Ganglia of Stressed Rats. in Acta Veterinaria, Beograd. 2010;60(1):15-22.
doi:10.2298/AVB1001015G .

Gavrilović, Ljubica, Spasojević, Nataša, Varagic, V., Dronjak, Slađana, "Gene Expression of Catecholamine Synthesizing Enzymes in Stellate Ganglia of Stressed Rats" in Acta Veterinaria, Beograd, 60, no. 1 (2010):15-22,
https://doi.org/10.2298/AVB1001015G . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Spasojević, Nataša
AU  - Dronjak, Slađana
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3966
AB  - Objective: Social isolation is regarded as one of the most relevant causes of diseases in mammalian species. The activation of the sympathoneural system represents one of the key components of the stress response. The sympathetic nervous system is one of the major pathways involved in immune-neuroendocrine interactions. The aim of the present study was to determine plasma epinephrine and norepinephrine in individually housed rats, as well as to find out whether splenic gene expression of catecholamine synthesizing enzymes and their protein levels are affected by chronic psychosocial stress. Methods: Tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) mRNA levels were quantified by quantitative real-time RT-PCR. The TH, DBH and PNMT immunoproteins were assayed by Western blot. Results: Chronic social isolation of adult male rats produced a significant increase in plasma catecholamine levels and a decrease in splenic TH mRNA, DBH mRNA and PNMT mRNA. Protein levels of TH, DBH and PNMT were also reduced. Conclusion: These results suggest that increased plasma catecholamines and decreased gene expression and protein levels of catecholamine biosynthetic enzymes in the spleen of chronically individually housed animals might reduce catecholamine synthesis, thus leaving the immunocompetent tissues depleted of catecholamines and consequently leading to an impairment of immune response. Copyright (C) 2010 S. Karger AG, Basel
T2  - Neuroimmunomodulation
T1  - Chronic Individual Housing-Induced Stress Decreased Expression of Catecholamine Biosynthetic Enzyme Genes and Proteins in Spleen of Adult Rats
VL  - 17
IS  - 4
SP  - 265
EP  - 269
DO  - 10.1159/000290042
ER  - 

@article{
author = "Gavrilović, Ljubica and Spasojević, Nataša and Dronjak, Slađana",
year = "2010",
abstract = "Objective: Social isolation is regarded as one of the most relevant causes of diseases in mammalian species. The activation of the sympathoneural system represents one of the key components of the stress response. The sympathetic nervous system is one of the major pathways involved in immune-neuroendocrine interactions. The aim of the present study was to determine plasma epinephrine and norepinephrine in individually housed rats, as well as to find out whether splenic gene expression of catecholamine synthesizing enzymes and their protein levels are affected by chronic psychosocial stress. Methods: Tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) mRNA levels were quantified by quantitative real-time RT-PCR. The TH, DBH and PNMT immunoproteins were assayed by Western blot. Results: Chronic social isolation of adult male rats produced a significant increase in plasma catecholamine levels and a decrease in splenic TH mRNA, DBH mRNA and PNMT mRNA. Protein levels of TH, DBH and PNMT were also reduced. Conclusion: These results suggest that increased plasma catecholamines and decreased gene expression and protein levels of catecholamine biosynthetic enzymes in the spleen of chronically individually housed animals might reduce catecholamine synthesis, thus leaving the immunocompetent tissues depleted of catecholamines and consequently leading to an impairment of immune response. Copyright (C) 2010 S. Karger AG, Basel",
journal = "Neuroimmunomodulation",
title = "Chronic Individual Housing-Induced Stress Decreased Expression of Catecholamine Biosynthetic Enzyme Genes and Proteins in Spleen of Adult Rats",
volume = "17",
number = "4",
pages = "265-269",
doi = "10.1159/000290042"
}

Gavrilović, L., Spasojević, N.,& Dronjak, S.. (2010). Chronic Individual Housing-Induced Stress Decreased Expression of Catecholamine Biosynthetic Enzyme Genes and Proteins in Spleen of Adult Rats. in Neuroimmunomodulation, 17(4), 265-269.
https://doi.org/10.1159/000290042

Gavrilović L, Spasojević N, Dronjak S. Chronic Individual Housing-Induced Stress Decreased Expression of Catecholamine Biosynthetic Enzyme Genes and Proteins in Spleen of Adult Rats. in Neuroimmunomodulation. 2010;17(4):265-269.
doi:10.1159/000290042 .

Gavrilović, Ljubica, Spasojević, Nataša, Dronjak, Slađana, "Chronic Individual Housing-Induced Stress Decreased Expression of Catecholamine Biosynthetic Enzyme Genes and Proteins in Spleen of Adult Rats" in Neuroimmunomodulation, 17, no. 4 (2010):265-269,
https://doi.org/10.1159/000290042 . .

TY  - JOUR
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Korićanac, Lela
AU  - Valastro, Lucia
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4101
AB  - Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was at its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer.
T2  - International Journal of Radiation Biology
T1  - Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak
VL  - 86
IS  - 9
SP  - 742
EP  - 751
DO  - 10.3109/09553002.2010.481322
ER  - 

@article{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V. and Korićanac, Lela and Valastro, Lucia and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo",
year = "2010",
abstract = "Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was at its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer.",
journal = "International Journal of Radiation Biology",
title = "Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak",
volume = "86",
number = "9",
pages = "742-751",
doi = "10.3109/09553002.2010.481322"
}

Petrović, I. M., Ristić-Fira, A., Todorović, D. V., Korićanac, L., Valastro, L., Cirrone, G. A. P.,& Cuttone, G.. (2010). Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak. in International Journal of Radiation Biology, 86(9), 742-751.
https://doi.org/10.3109/09553002.2010.481322

Petrović IM, Ristić-Fira A, Todorović DV, Korićanac L, Valastro L, Cirrone GAP, Cuttone G. Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak. in International Journal of Radiation Biology. 2010;86(9):742-751.
doi:10.3109/09553002.2010.481322 .

Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., Korićanac, Lela, Valastro, Lucia, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak" in International Journal of Radiation Biology, 86, no. 9 (2010):742-751,
https://doi.org/10.3109/09553002.2010.481322 . .

TY  - JOUR
AU  - Filipović, Dragana
AU  - Mandic, Ljuba M.
AU  - Kanazir, Dušan T.
AU  - Pajović, Snežana B.
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3979
AB  - Cellular protection against oxidative stress is afforded by the enzyme superoxide dismutase (SOD). In this study, the protein levels of copper-zinc SOD (CuZnSOD) in the cytosolic and nuclear fraction, manganese SOD (MnSOD) in the mitochondrial, and cytosolic fraction and cytochrome c (cyt c) in the liver of male rats exposed to 2 h of acute immobilization (IM) or Cold stress, 21 days chronic isolation or their combinations (chronic/acute stress) were examined. The serum corticosterone (CORT) level was measured, as an indicator of stress stimuli. Both acute stressors with elevated CORT levels caused a decrease of mitochondrial MnSOD, while acute IM resulted in redistribution of the CuZnSOD protein level between the cytosolic and nuclear fraction. Chronic isolation, during which the CORT level was close to control value, resulted in an increase of cytosolic CuZnSOD, whereas a decrease of MnSOD in mitochondrial and its corresponding increase in cytosol fraction was found. In both combined stress regimes, an increase of the CuZnSOD and MnSOD levels in the cytosolic fraction was recorded whereby increase of the CORT level was observed only in the chronic isolation followed by acute IM. The data indicate that acute and/or chronic stress models have different degrees of influence on serum CORT and SOD subcellular protein levels. Increased cytosolic CuZnSOD protein level under chronic isolation suggests that state of oxidative stress may also exist under CORT level similar to the basal value. The presence of MnSOD and cyt c in the cytosolic fraction could serve as useful parameters for mitochondrial dysfunction.
T2  - Molecular and Cellular Biochemistry
T1  - Acute and/or chronic stress models modulate CuZnSOD and MnSOD protein expression in rat liver
VL  - 338
IS  - 1-2
SP  - 167
EP  - 174
DO  - 10.1007/s11010-009-0350-8
ER  - 

@article{
author = "Filipović, Dragana and Mandic, Ljuba M. and Kanazir, Dušan T. and Pajović, Snežana B.",
year = "2010",
abstract = "Cellular protection against oxidative stress is afforded by the enzyme superoxide dismutase (SOD). In this study, the protein levels of copper-zinc SOD (CuZnSOD) in the cytosolic and nuclear fraction, manganese SOD (MnSOD) in the mitochondrial, and cytosolic fraction and cytochrome c (cyt c) in the liver of male rats exposed to 2 h of acute immobilization (IM) or Cold stress, 21 days chronic isolation or their combinations (chronic/acute stress) were examined. The serum corticosterone (CORT) level was measured, as an indicator of stress stimuli. Both acute stressors with elevated CORT levels caused a decrease of mitochondrial MnSOD, while acute IM resulted in redistribution of the CuZnSOD protein level between the cytosolic and nuclear fraction. Chronic isolation, during which the CORT level was close to control value, resulted in an increase of cytosolic CuZnSOD, whereas a decrease of MnSOD in mitochondrial and its corresponding increase in cytosol fraction was found. In both combined stress regimes, an increase of the CuZnSOD and MnSOD levels in the cytosolic fraction was recorded whereby increase of the CORT level was observed only in the chronic isolation followed by acute IM. The data indicate that acute and/or chronic stress models have different degrees of influence on serum CORT and SOD subcellular protein levels. Increased cytosolic CuZnSOD protein level under chronic isolation suggests that state of oxidative stress may also exist under CORT level similar to the basal value. The presence of MnSOD and cyt c in the cytosolic fraction could serve as useful parameters for mitochondrial dysfunction.",
journal = "Molecular and Cellular Biochemistry",
title = "Acute and/or chronic stress models modulate CuZnSOD and MnSOD protein expression in rat liver",
volume = "338",
number = "1-2",
pages = "167-174",
doi = "10.1007/s11010-009-0350-8"
}

Filipović, D., Mandic, L. M., Kanazir, D. T.,& Pajović, S. B.. (2010). Acute and/or chronic stress models modulate CuZnSOD and MnSOD protein expression in rat liver. in Molecular and Cellular Biochemistry, 338(1-2), 167-174.
https://doi.org/10.1007/s11010-009-0350-8

Filipović D, Mandic LM, Kanazir DT, Pajović SB. Acute and/or chronic stress models modulate CuZnSOD and MnSOD protein expression in rat liver. in Molecular and Cellular Biochemistry. 2010;338(1-2):167-174.
doi:10.1007/s11010-009-0350-8 .

Filipović, Dragana, Mandic, Ljuba M., Kanazir, Dušan T., Pajović, Snežana B., "Acute and/or chronic stress models modulate CuZnSOD and MnSOD protein expression in rat liver" in Molecular and Cellular Biochemistry, 338, no. 1-2 (2010):167-174,
https://doi.org/10.1007/s11010-009-0350-8 . .

TY  - JOUR
AU  - Horvat, Anica
AU  - Milošević, Maja
AU  - Demajo, Miroslav
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3630
AB  - In this study, we investigated the in vitro effects of metal ions on Mg2+-ATPase activity in isolated membranes from rat uterus. The effects of increasing concentrations of metal salts (CrCl2, CuSO4, HgCl2 and ZnSO4) show sigmoidal and almost complete inhibition relative to the control enzyme activity. According to the IC50, the ATPase possesses greater sensibility to Zn2+ GT Cu2+ congruent to Cr3+ congruent to Hg2+, while other metal salts exhibit the following inhibition: CdCl2 55%, CsCl 64.5% and SrCl2 58%. Here we demonstrated that the physicochemical properties of these metals are of importance in defining possible mechanisms of binding and decrease of enzyme activity.
T2  - Environmental Chemistry Letters
T1  - Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus
VL  - 7
IS  - 1
SP  - 41
EP  - 44
DO  - 10.1007/s10311-007-0132-z
ER  - 

@article{
author = "Horvat, Anica and Milošević, Maja and Demajo, Miroslav",
year = "2009",
abstract = "In this study, we investigated the in vitro effects of metal ions on Mg2+-ATPase activity in isolated membranes from rat uterus. The effects of increasing concentrations of metal salts (CrCl2, CuSO4, HgCl2 and ZnSO4) show sigmoidal and almost complete inhibition relative to the control enzyme activity. According to the IC50, the ATPase possesses greater sensibility to Zn2+ GT Cu2+ congruent to Cr3+ congruent to Hg2+, while other metal salts exhibit the following inhibition: CdCl2 55%, CsCl 64.5% and SrCl2 58%. Here we demonstrated that the physicochemical properties of these metals are of importance in defining possible mechanisms of binding and decrease of enzyme activity.",
journal = "Environmental Chemistry Letters",
title = "Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus",
volume = "7",
number = "1",
pages = "41-44",
doi = "10.1007/s10311-007-0132-z"
}

Horvat, A., Milošević, M.,& Demajo, M.. (2009). Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus. in Environmental Chemistry Letters, 7(1), 41-44.
https://doi.org/10.1007/s10311-007-0132-z

Horvat A, Milošević M, Demajo M. Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus. in Environmental Chemistry Letters. 2009;7(1):41-44.
doi:10.1007/s10311-007-0132-z .

Horvat, Anica, Milošević, Maja, Demajo, Miroslav, "Effects of metal ions on Mg2+-ATPase activity in plasma membranes isolated from the rat uterus" in Environmental Chemistry Letters, 7, no. 1 (2009):41-44,
https://doi.org/10.1007/s10311-007-0132-z . .

TY  - JOUR
AU  - Petrović, Snježana
AU  - Milošević, Maja
AU  - Stanojević, Ivana
AU  - Velickovic, Natasa
AU  - Drakulić, Dunja R.
AU  - Horvat, Anica
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3749
AB  - The role of membrane-bound estradiol in modulation of mitochondrial Ca2+ flux in nerve endings isolated from rat brain stem was examined. Physiological concentrations of 17 beta-estradiol bind specifically to isolated mitochondria (Bmax 33.8 +/- 2.5 fmoles estradiol/mg of protein, Km 0.185 +/- 0.006 nmoles/l free estradiol). At concentrations ranging from 1 x 10-10 to 2 x 10-9 moles/l, estradiol significantly (by 23-28%) decreases mitochondrial Na-dependent calcium efflux. Decreased calcium efflux was associated with increased affinity of the Na+/Ca2+ exchanger for Na+ and decreased capacity of the exchanger to extrude Ca2+. Calcium ion efflux modulation and mitochondrial ion retention may be the way that 17 beta-estradiol exerts its role in nerve cell homeostasis.
T2  - Archives of Biological Sciences
T1  - Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol
VL  - 61
IS  - 2
SP  - 171
EP  - 177
DO  - 10.2298/ABS0902171P
ER  - 

@article{
author = "Petrović, Snježana and Milošević, Maja and Stanojević, Ivana and Velickovic, Natasa and Drakulić, Dunja R. and Horvat, Anica",
year = "2009",
abstract = "The role of membrane-bound estradiol in modulation of mitochondrial Ca2+ flux in nerve endings isolated from rat brain stem was examined. Physiological concentrations of 17 beta-estradiol bind specifically to isolated mitochondria (Bmax 33.8 +/- 2.5 fmoles estradiol/mg of protein, Km 0.185 +/- 0.006 nmoles/l free estradiol). At concentrations ranging from 1 x 10-10 to 2 x 10-9 moles/l, estradiol significantly (by 23-28%) decreases mitochondrial Na-dependent calcium efflux. Decreased calcium efflux was associated with increased affinity of the Na+/Ca2+ exchanger for Na+ and decreased capacity of the exchanger to extrude Ca2+. Calcium ion efflux modulation and mitochondrial ion retention may be the way that 17 beta-estradiol exerts its role in nerve cell homeostasis.",
journal = "Archives of Biological Sciences",
title = "Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol",
volume = "61",
number = "2",
pages = "171-177",
doi = "10.2298/ABS0902171P"
}

Petrović, S., Milošević, M., Stanojević, I., Velickovic, N., Drakulić, D. R.,& Horvat, A.. (2009). Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol. in Archives of Biological Sciences, 61(2), 171-177.
https://doi.org/10.2298/ABS0902171P

Petrović S, Milošević M, Stanojević I, Velickovic N, Drakulić DR, Horvat A. Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol. in Archives of Biological Sciences. 2009;61(2):171-177.
doi:10.2298/ABS0902171P .

Petrović, Snježana, Milošević, Maja, Stanojević, Ivana, Velickovic, Natasa, Drakulić, Dunja R., Horvat, Anica, "Inhibition of Mitochondrial Na-Dependent Ca2+ Efflux from Rat Brain Stem By 17 Beta-Estradiol" in Archives of Biological Sciences, 61, no. 2 (2009):171-177,
https://doi.org/10.2298/ABS0902171P . .

TY  - JOUR
AU  - Velickovic, Natasa
AU  - Đorđević, Ana D.
AU  - Drakulić, Dunja R.
AU  - Stanojević, Ivana
AU  - Šećerov, Bojana Lj.
AU  - Horvat, Anica
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2559
AB  - Glucocorticoids, essential for normal,hypothalamic-pituitary-adrenal (HPA) axis activity, exert their action on the hippocampus through two types of corticosteroid receptors: the glucocorticoid receptor (GR) and the mineralocorticoid receptor (MR). Recent studies report that exposure of juvenile rats to cranial irradiation adversely affects HPA axis stability leading to its activation along with radiation-induced inflammation. This study was aimed to examine the acute effects of radiation on HPA axis activity and hippocampal corticosteroid receptor expression in 18-day-old rats. Since immobilization was part of irradiation procedure, both irradiated and sham-irradiated animals were exposed to this unavoidable stress. Our results demonstrate that the irradiated rats exhibited different pattern of corticosteroid receptor expression and hormone levels compared to respective controls. These differences included upregulation of GR protein in the hippocampus with a concomitant elevation of GR mRNA and an increase in circulating level of corticosterone. In addition, the expression of MR, both at the level of protein and gene expression, was not altered. Taken together, this study demonstrates that cranial irradiation in juvenile rats leads to enhanced HPA axis activity and increased relative GR/MR ratio in hippocampus. The present paper intends to show that neuroendocrine response of normal brain tissue to localized irradiation comprise both activation of HPA axis and altered corticosteroid receptor balance, probably as consequence of innate immune activation.
T2  - General Physiology and Biophysics
T1  - Cranial irradiation modulates hypothalamic-pituitary-adrenal axis activity and corticosteroid receptor expression in the hippocampus of juvenile rat
VL  - 28
IS  - SI
SP  - 219
EP  - 227
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2559
ER  - 

@article{
author = "Velickovic, Natasa and Đorđević, Ana D. and Drakulić, Dunja R. and Stanojević, Ivana and Šećerov, Bojana Lj. and Horvat, Anica",
year = "2009",
abstract = "Glucocorticoids, essential for normal,hypothalamic-pituitary-adrenal (HPA) axis activity, exert their action on the hippocampus through two types of corticosteroid receptors: the glucocorticoid receptor (GR) and the mineralocorticoid receptor (MR). Recent studies report that exposure of juvenile rats to cranial irradiation adversely affects HPA axis stability leading to its activation along with radiation-induced inflammation. This study was aimed to examine the acute effects of radiation on HPA axis activity and hippocampal corticosteroid receptor expression in 18-day-old rats. Since immobilization was part of irradiation procedure, both irradiated and sham-irradiated animals were exposed to this unavoidable stress. Our results demonstrate that the irradiated rats exhibited different pattern of corticosteroid receptor expression and hormone levels compared to respective controls. These differences included upregulation of GR protein in the hippocampus with a concomitant elevation of GR mRNA and an increase in circulating level of corticosterone. In addition, the expression of MR, both at the level of protein and gene expression, was not altered. Taken together, this study demonstrates that cranial irradiation in juvenile rats leads to enhanced HPA axis activity and increased relative GR/MR ratio in hippocampus. The present paper intends to show that neuroendocrine response of normal brain tissue to localized irradiation comprise both activation of HPA axis and altered corticosteroid receptor balance, probably as consequence of innate immune activation.",
journal = "General Physiology and Biophysics",
title = "Cranial irradiation modulates hypothalamic-pituitary-adrenal axis activity and corticosteroid receptor expression in the hippocampus of juvenile rat",
volume = "28",
number = "SI",
pages = "219-227",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2559"
}

Velickovic, N., Đorđević, A. D., Drakulić, D. R., Stanojević, I., Šećerov, B. Lj.,& Horvat, A.. (2009). Cranial irradiation modulates hypothalamic-pituitary-adrenal axis activity and corticosteroid receptor expression in the hippocampus of juvenile rat. in General Physiology and Biophysics, 28(SI), 219-227.
https://hdl.handle.net/21.15107/rcub_vinar_2559

Velickovic N, Đorđević AD, Drakulić DR, Stanojević I, Šećerov BL, Horvat A. Cranial irradiation modulates hypothalamic-pituitary-adrenal axis activity and corticosteroid receptor expression in the hippocampus of juvenile rat. in General Physiology and Biophysics. 2009;28(SI):219-227.
https://hdl.handle.net/21.15107/rcub_vinar_2559 .

Velickovic, Natasa, Đorđević, Ana D., Drakulić, Dunja R., Stanojević, Ivana, Šećerov, Bojana Lj., Horvat, Anica, "Cranial irradiation modulates hypothalamic-pituitary-adrenal axis activity and corticosteroid receptor expression in the hippocampus of juvenile rat" in General Physiology and Biophysics, 28, no. SI (2009):219-227,
https://hdl.handle.net/21.15107/rcub_vinar_2559 .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Spasojević, Nataša
AU  - Dronjak, Slađana
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3252
AB  - In this study we investigated the changes in norepinephrine biosynthetic enzymes tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) gene expression in the stellate ganglia of naive controls and long-term socially isolated (12 weeks) adult rats and the response of these animals to additional immobilization stress. Psychosocial stress produced a significant increase of both TH mRNA and DBH mRNA levels in stellate ganglia. Additional immobilization of long-term psychosocially stressed rats expressed no effect on gene expression of these enzymes. The results presented here suggest that psychosocial stress-induced increase in gene expression of norepinephrine biosynthetic enzymes in stellate ganglia may be connected to the increased risk of cardiovascular disease. (C) 2009 Elsevier B.V. All rights reserved.
T2  - Autonomic Neuroscience: Basic and Clinical
T1  - Psychosocial stress-related changes in gene expression of norepinephrine biosynthetic enzymes in stellate ganglia of adult rats
VL  - 150
IS  - 1-2
SP  - 144
EP  - 146
DO  - 10.1016/j.autneu.2009.05.242
ER  - 

@article{
author = "Gavrilović, Ljubica and Spasojević, Nataša and Dronjak, Slađana",
year = "2009",
abstract = "In this study we investigated the changes in norepinephrine biosynthetic enzymes tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) gene expression in the stellate ganglia of naive controls and long-term socially isolated (12 weeks) adult rats and the response of these animals to additional immobilization stress. Psychosocial stress produced a significant increase of both TH mRNA and DBH mRNA levels in stellate ganglia. Additional immobilization of long-term psychosocially stressed rats expressed no effect on gene expression of these enzymes. The results presented here suggest that psychosocial stress-induced increase in gene expression of norepinephrine biosynthetic enzymes in stellate ganglia may be connected to the increased risk of cardiovascular disease. (C) 2009 Elsevier B.V. All rights reserved.",
journal = "Autonomic Neuroscience: Basic and Clinical",
title = "Psychosocial stress-related changes in gene expression of norepinephrine biosynthetic enzymes in stellate ganglia of adult rats",
volume = "150",
number = "1-2",
pages = "144-146",
doi = "10.1016/j.autneu.2009.05.242"
}

Gavrilović, L., Spasojević, N.,& Dronjak, S.. (2009). Psychosocial stress-related changes in gene expression of norepinephrine biosynthetic enzymes in stellate ganglia of adult rats. in Autonomic Neuroscience: Basic and Clinical, 150(1-2), 144-146.
https://doi.org/10.1016/j.autneu.2009.05.242

Gavrilović L, Spasojević N, Dronjak S. Psychosocial stress-related changes in gene expression of norepinephrine biosynthetic enzymes in stellate ganglia of adult rats. in Autonomic Neuroscience: Basic and Clinical. 2009;150(1-2):144-146.
doi:10.1016/j.autneu.2009.05.242 .

Gavrilović, Ljubica, Spasojević, Nataša, Dronjak, Slađana, "Psychosocial stress-related changes in gene expression of norepinephrine biosynthetic enzymes in stellate ganglia of adult rats" in Autonomic Neuroscience: Basic and Clinical, 150, no. 1-2 (2009):144-146,
https://doi.org/10.1016/j.autneu.2009.05.242 . .

TY  - JOUR
AU  - Gavrilović, Ljubica
AU  - Spasojević, Nataša
AU  - Živković, Maja
AU  - Dronjak, Slađana
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3845
AB  - Chronic stress is associated with the development of cardiovascular diseases. The sympathoneural system plays an important role in the regulation of cardiac function both in health and disease. In the present study, the changes in gene expression of the catecholamine biosynthetic enzymes tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) and protein levels in the right and left heart auricles of naive control and long-term (12 weeks) socially isolated rats were investigated by Taqman RT-PCR and Western blot analysis. The response of these animals to additional immobilization stress (2 h) was also examined. Long-term social isolation produced a decrease in TH mRNA level in left auricles (about 70%) compared to the corresponding control. Expression of the DBH gene was markedly decreased both in the right (about 62%) and left (about 81%) auricles compared to the corresponding control, group-maintained rats, whereas PNMT mRNA levels remained unchanged. Exposure of group-housed rats to acute immobilization for 2 h led to a significant increase of mRNA levels of TH (about 267%), DBH (about 37%) and PNMT ( about 60%) only in the right auricles. Additional 2-h immobilization of individually housed rats did not affect gene expression of these enzymes in either the right or left auricle. Protein levels of TH, DBH and PNMT in left and right heart auricles were unchanged either in both individually housed and immobilized rats. The unchanged mRNA levels of the enzymes examined after short-term immobilization suggest that the catecholaminergic system of the heart auricles of animals previously exposed to chronic psychosocial stress was adapted to maintain appropriate cardiovascular homeostasis.
T2  - Brazilian Journal of Medical and Biological Research
T1  - Effect of immobilization stress on gene expression of catecholamine biosynthetic enzymes in heart auricles of socially isolated rats
VL  - 42
IS  - 12
SP  - 1185
EP  - 1190
DO  - 10.1590/S0100-879X2009005000040
ER  - 

@article{
author = "Gavrilović, Ljubica and Spasojević, Nataša and Živković, Maja and Dronjak, Slađana",
year = "2009",
abstract = "Chronic stress is associated with the development of cardiovascular diseases. The sympathoneural system plays an important role in the regulation of cardiac function both in health and disease. In the present study, the changes in gene expression of the catecholamine biosynthetic enzymes tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) and protein levels in the right and left heart auricles of naive control and long-term (12 weeks) socially isolated rats were investigated by Taqman RT-PCR and Western blot analysis. The response of these animals to additional immobilization stress (2 h) was also examined. Long-term social isolation produced a decrease in TH mRNA level in left auricles (about 70%) compared to the corresponding control. Expression of the DBH gene was markedly decreased both in the right (about 62%) and left (about 81%) auricles compared to the corresponding control, group-maintained rats, whereas PNMT mRNA levels remained unchanged. Exposure of group-housed rats to acute immobilization for 2 h led to a significant increase of mRNA levels of TH (about 267%), DBH (about 37%) and PNMT ( about 60%) only in the right auricles. Additional 2-h immobilization of individually housed rats did not affect gene expression of these enzymes in either the right or left auricle. Protein levels of TH, DBH and PNMT in left and right heart auricles were unchanged either in both individually housed and immobilized rats. The unchanged mRNA levels of the enzymes examined after short-term immobilization suggest that the catecholaminergic system of the heart auricles of animals previously exposed to chronic psychosocial stress was adapted to maintain appropriate cardiovascular homeostasis.",
journal = "Brazilian Journal of Medical and Biological Research",
title = "Effect of immobilization stress on gene expression of catecholamine biosynthetic enzymes in heart auricles of socially isolated rats",
volume = "42",
number = "12",
pages = "1185-1190",
doi = "10.1590/S0100-879X2009005000040"
}

Gavrilović, L., Spasojević, N., Živković, M.,& Dronjak, S.. (2009). Effect of immobilization stress on gene expression of catecholamine biosynthetic enzymes in heart auricles of socially isolated rats. in Brazilian Journal of Medical and Biological Research, 42(12), 1185-1190.
https://doi.org/10.1590/S0100-879X2009005000040

Gavrilović L, Spasojević N, Živković M, Dronjak S. Effect of immobilization stress on gene expression of catecholamine biosynthetic enzymes in heart auricles of socially isolated rats. in Brazilian Journal of Medical and Biological Research. 2009;42(12):1185-1190.
doi:10.1590/S0100-879X2009005000040 .

Gavrilović, Ljubica, Spasojević, Nataša, Živković, Maja, Dronjak, Slađana, "Effect of immobilization stress on gene expression of catecholamine biosynthetic enzymes in heart auricles of socially isolated rats" in Brazilian Journal of Medical and Biological Research, 42, no. 12 (2009):1185-1190,
https://doi.org/10.1590/S0100-879X2009005000040 . .

TY  - JOUR
AU  - Filipović, Dragana
AU  - Martinović, Jelena
AU  - Pajović, Snežana B.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2558
AB  - Manganese superoxide dismutase (MnSOD) is the major antioxidant in mitochondria that protect brain from neuroendocrine stress. Although MnSOD is localized in the mitochondria, the immediate subcellular distribution of MnSOD protein level in the prefrontal cortex and hippocampus of Wistar male rats exposed to acute stressors immobilization or cold, chronic stress isolation or their combinations (acute/chronic) have not been studied. Western immunoblotting revealed that acute immobilization stress resulted in an increase in mitochondria! MnSOD protein level, whereas chronic isolation compromises MnSOD protein level. Chronically stressed animals exposed to novel acute stressors showed a significant decrease in mitochondria MnSOD protein level and reciprocal increase in this protein in the cytosolic fraction. At the same time, a significant increase in serum corticosterone level was observed after acute stressors, whereas chronic isolation led to negligable changes and caused a reduced responsiveness to a novel acute stressors. Presence of cytochrome c in mitochondrial and cytosolic fraction of both brain structures was also confirmed. Results suggest that chronic stress isolation results in mitochondrial dysfunction and MnSOD release into the cytosol.
T2  - General Physiology and Biophysics
T1  - The effect of acute or/and chronic stress on the MnSOD protein expression in rat prefrontal cortex and hippocampus
VL  - 28
IS  - SI
SP  - 53
EP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2558
ER  - 

@article{
author = "Filipović, Dragana and Martinović, Jelena and Pajović, Snežana B.",
year = "2009",
abstract = "Manganese superoxide dismutase (MnSOD) is the major antioxidant in mitochondria that protect brain from neuroendocrine stress. Although MnSOD is localized in the mitochondria, the immediate subcellular distribution of MnSOD protein level in the prefrontal cortex and hippocampus of Wistar male rats exposed to acute stressors immobilization or cold, chronic stress isolation or their combinations (acute/chronic) have not been studied. Western immunoblotting revealed that acute immobilization stress resulted in an increase in mitochondria! MnSOD protein level, whereas chronic isolation compromises MnSOD protein level. Chronically stressed animals exposed to novel acute stressors showed a significant decrease in mitochondria MnSOD protein level and reciprocal increase in this protein in the cytosolic fraction. At the same time, a significant increase in serum corticosterone level was observed after acute stressors, whereas chronic isolation led to negligable changes and caused a reduced responsiveness to a novel acute stressors. Presence of cytochrome c in mitochondrial and cytosolic fraction of both brain structures was also confirmed. Results suggest that chronic stress isolation results in mitochondrial dysfunction and MnSOD release into the cytosol.",
journal = "General Physiology and Biophysics",
title = "The effect of acute or/and chronic stress on the MnSOD protein expression in rat prefrontal cortex and hippocampus",
volume = "28",
number = "SI",
pages = "53-61",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2558"
}

Filipović, D., Martinović, J.,& Pajović, S. B.. (2009). The effect of acute or/and chronic stress on the MnSOD protein expression in rat prefrontal cortex and hippocampus. in General Physiology and Biophysics, 28(SI), 53-61.
https://hdl.handle.net/21.15107/rcub_vinar_2558

Filipović D, Martinović J, Pajović SB. The effect of acute or/and chronic stress on the MnSOD protein expression in rat prefrontal cortex and hippocampus. in General Physiology and Biophysics. 2009;28(SI):53-61.
https://hdl.handle.net/21.15107/rcub_vinar_2558 .

Filipović, Dragana, Martinović, Jelena, Pajović, Snežana B., "The effect of acute or/and chronic stress on the MnSOD protein expression in rat prefrontal cortex and hippocampus" in General Physiology and Biophysics, 28, no. SI (2009):53-61,
https://hdl.handle.net/21.15107/rcub_vinar_2558 .

TY  - JOUR
AU  - Filipović, Dragana
AU  - Pajović, Snežana B.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3724
AB  - Neuroendocrine stress (NES) causes increase of glucocorticoids and alters physiological levels of reactive oxygen species production in cells, which might involve modifications in the antioxidant defense system. We investigated the hypothesis that acute, chronic, or combined stress alters copper-zinc superoxide dismutase (CuZnSOD) expression pattern at both, mRNA and subcellular protein level in the cerebral cortex and hippocampus of rats and that there may be a relationship between stress-induced corticosterone and CuZnSOD expression. The most effective stress model which led to the most pronounced changes in CuZnSOD expression patterns was also investigated. Our results demonstrated that acute stress immobilization up-regulates mRNA expression of hippocampal CuZnSOD, while cytosolic protein expression of this enzyme was increased in both brain structures. Chronic stress isolation had no effect on either mRNA and protein expression level and caused a lack of significant up-regulation to a novel acute stressors. The presence of this protein in nuclear fractions of both brain structures was also confirmed. The elevated cytosolic CuZnSOD protein levels following acute immobilization might reflect on the defense system against oxidative stress. Chronic isolation compromises CuZnSOD protein expression, which may lead to the inefficient defense against reactive oxygen species (ROS). The stress-triggered CuZnSOD protein expression was not correlated by the corresponding mRNA. The results suggest that different stress models exert a different degree of influence on mRNA and protein level of CuZnSOD in both brain structures as well as serum corticosterone.
T2  - Cellular and Molecular Neurobiology
T1  - Differential Regulation of CuZnSOD Expression in Rat Brain by Acute and/or Chronic Stress
VL  - 29
IS  - 5
SP  - 673
EP  - 681
DO  - 10.1007/s10571-009-9375-5
ER  - 

@article{
author = "Filipović, Dragana and Pajović, Snežana B.",
year = "2009",
abstract = "Neuroendocrine stress (NES) causes increase of glucocorticoids and alters physiological levels of reactive oxygen species production in cells, which might involve modifications in the antioxidant defense system. We investigated the hypothesis that acute, chronic, or combined stress alters copper-zinc superoxide dismutase (CuZnSOD) expression pattern at both, mRNA and subcellular protein level in the cerebral cortex and hippocampus of rats and that there may be a relationship between stress-induced corticosterone and CuZnSOD expression. The most effective stress model which led to the most pronounced changes in CuZnSOD expression patterns was also investigated. Our results demonstrated that acute stress immobilization up-regulates mRNA expression of hippocampal CuZnSOD, while cytosolic protein expression of this enzyme was increased in both brain structures. Chronic stress isolation had no effect on either mRNA and protein expression level and caused a lack of significant up-regulation to a novel acute stressors. The presence of this protein in nuclear fractions of both brain structures was also confirmed. The elevated cytosolic CuZnSOD protein levels following acute immobilization might reflect on the defense system against oxidative stress. Chronic isolation compromises CuZnSOD protein expression, which may lead to the inefficient defense against reactive oxygen species (ROS). The stress-triggered CuZnSOD protein expression was not correlated by the corresponding mRNA. The results suggest that different stress models exert a different degree of influence on mRNA and protein level of CuZnSOD in both brain structures as well as serum corticosterone.",
journal = "Cellular and Molecular Neurobiology",
title = "Differential Regulation of CuZnSOD Expression in Rat Brain by Acute and/or Chronic Stress",
volume = "29",
number = "5",
pages = "673-681",
doi = "10.1007/s10571-009-9375-5"
}

Filipović, D.,& Pajović, S. B.. (2009). Differential Regulation of CuZnSOD Expression in Rat Brain by Acute and/or Chronic Stress. in Cellular and Molecular Neurobiology, 29(5), 673-681.
https://doi.org/10.1007/s10571-009-9375-5

Filipović D, Pajović SB. Differential Regulation of CuZnSOD Expression in Rat Brain by Acute and/or Chronic Stress. in Cellular and Molecular Neurobiology. 2009;29(5):673-681.
doi:10.1007/s10571-009-9375-5 .

Filipović, Dragana, Pajović, Snežana B., "Differential Regulation of CuZnSOD Expression in Rat Brain by Acute and/or Chronic Stress" in Cellular and Molecular Neurobiology, 29, no. 5 (2009):673-681,
https://doi.org/10.1007/s10571-009-9375-5 . .

TY  - CONF
AU  - Petrović, S.
AU  - Milošević, Maja
AU  - Horvat, Anica
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9368
AB  - In the present study the modulation of Ca2+ ion flux in the synaptosomal mitochondria isolated from the ovariectyomized rat Brain Steam and the possible roll of membrane bound estradiol was examined. Physiological concentrations of 17β-estradiol binds specifically to isolated mitochondria (Vmax 3.37± 0.25 pmol/mg protein, Km 1.85± 0.06 nmol/l of free estradiol). Addition of 17β-estradiol (10 pmol/l - 1 nmol/ l) in vitro decreased mitochondrial calcium ion efflux significantly (25%) after 10 minutes. Modulation of calcium ion efflux and mitochondrial ion retention may be the way that 17β-estradiol (E2) exerts its role in the nerve cell homeostasis.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
T1  - Modulation of Ca2+ ion flux through mitochondrial membrane of the rat brain steam synaptosomes by 17β-estradiol
VL  - 1
SP  - 352
EP  - 354
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9368
ER  - 

@conference{
author = "Petrović, S. and Milošević, Maja and Horvat, Anica",
year = "2008",
abstract = "In the present study the modulation of Ca2+ ion flux in the synaptosomal mitochondria isolated from the ovariectyomized rat Brain Steam and the possible roll of membrane bound estradiol was examined. Physiological concentrations of 17β-estradiol binds specifically to isolated mitochondria (Vmax 3.37± 0.25 pmol/mg protein, Km 1.85± 0.06 nmol/l of free estradiol). Addition of 17β-estradiol (10 pmol/l - 1 nmol/ l) in vitro decreased mitochondrial calcium ion efflux significantly (25%) after 10 minutes. Modulation of calcium ion efflux and mitochondrial ion retention may be the way that 17β-estradiol (E2) exerts its role in the nerve cell homeostasis.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry",
title = "Modulation of Ca2+ ion flux through mitochondrial membrane of the rat brain steam synaptosomes by 17β-estradiol",
volume = "1",
pages = "352-354",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9368"
}

Petrović, S., Milošević, M.,& Horvat, A.. (2008). Modulation of Ca2+ ion flux through mitochondrial membrane of the rat brain steam synaptosomes by 17β-estradiol. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 352-354.
https://hdl.handle.net/21.15107/rcub_vinar_9368

Petrović S, Milošević M, Horvat A. Modulation of Ca2+ ion flux through mitochondrial membrane of the rat brain steam synaptosomes by 17β-estradiol. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry. 2008;1:352-354.
https://hdl.handle.net/21.15107/rcub_vinar_9368 .

Petrović, S., Milošević, Maja, Horvat, Anica, "Modulation of Ca2+ ion flux through mitochondrial membrane of the rat brain steam synaptosomes by 17β-estradiol" in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry, 1 (2008):352-354,
https://hdl.handle.net/21.15107/rcub_vinar_9368 .

TY  - CONF
AU  - Milošević, Maja
AU  - Petrović, S.
AU  - Horvat, Anica
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9379
AB  - The potential protective effects of ethylenediamine tetraacetic acid (EDTA) on the CuSO4 cell toxicity was examined on rat myometrial plasma membranes. Activity of plasma membrane ecto-ATPase, as modulator of purinergic signaling in the presence of increasing concentrations of copper salt and in the presence or absence of EDTA was studied. Our results show that 1mmol/l EDTA may exert protective effects on Cu2+-induced toxicity by increasing half-maximum inhibitory activities of this ion.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
T1  - Protection of copper ions induced inhibition of rat myometrial ecto-atpase activity by edta
VL  - 1
SP  - 444
EP  - 446
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9379
ER  - 

@conference{
author = "Milošević, Maja and Petrović, S. and Horvat, Anica",
year = "2008",
abstract = "The potential protective effects of ethylenediamine tetraacetic acid (EDTA) on the CuSO4 cell toxicity was examined on rat myometrial plasma membranes. Activity of plasma membrane ecto-ATPase, as modulator of purinergic signaling in the presence of increasing concentrations of copper salt and in the presence or absence of EDTA was studied. Our results show that 1mmol/l EDTA may exert protective effects on Cu2+-induced toxicity by increasing half-maximum inhibitory activities of this ion.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry",
title = "Protection of copper ions induced inhibition of rat myometrial ecto-atpase activity by edta",
volume = "1",
pages = "444-446",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9379"
}

Milošević, M., Petrović, S.,& Horvat, A.. (2008). Protection of copper ions induced inhibition of rat myometrial ecto-atpase activity by edta. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 444-446.
https://hdl.handle.net/21.15107/rcub_vinar_9379

Milošević M, Petrović S, Horvat A. Protection of copper ions induced inhibition of rat myometrial ecto-atpase activity by edta. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry. 2008;1:444-446.
https://hdl.handle.net/21.15107/rcub_vinar_9379 .

Milošević, Maja, Petrović, S., Horvat, Anica, "Protection of copper ions induced inhibition of rat myometrial ecto-atpase activity by edta" in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry, 1 (2008):444-446,
https://hdl.handle.net/21.15107/rcub_vinar_9379 .