TY  - JOUR
AU  - Korać Jačić, Jelena
AU  - Nikolić, Ljiljana
AU  - Stanković, Dalibor M.
AU  - Opačić, Miloš
AU  - Dimitrijević, Milena S.
AU  - Savić, Danijela
AU  - Grgurić-Šipka, Sanja
AU  - Spasojević, Ivan
AU  - Bogdanović-Pristov, Jelena
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8801
AB  - Upon release in response to stress, epinephrine (Epi) may interact with labile iron pool in human plasma with potentially important (patho)physiological consequences. We have shown that Epi and Fe3+ build stable 1:1 high-spin bidentate complex at physiological pH, and that Epi does not undergo degradation in the presence of iron. However, the interactions of Epi with the more soluble Fe2+, and the impact of iron on biological activity of Epi are still not known. Herein we showed that Epi and Fe2+ build colorless complex which is stable under anaerobic conditions. In the presence of O2, Epi promoted the oxidation of Fe2+ and the formation of Epi-Fe3+ complex. Cyclic voltammetry showed that mid-point potential of Epi-Fe2+ complex is very low (-582 mV vs. standard hydrogen electrode), which explains catalyzed oxidation of Fe2+. Next, we examined the impact of iron binding on biological performance of Epi using patch clamping in cell culture with constitutive expression of adrenergic receptors. Epi alone evoked an increase of outward currents, whereas Epi in the complex with Fe3+ did not. This implies that the binding of Epi to adrenergic receptors and their activation is prevented by the formation of complex with iron. Pro-oxidative activity of Epi-Fe2+ complex may represent a link between chronic stress and cardiovascular problems. On the other hand, labile iron could serve as a modulator of biological activity of ligands. Such interactions may be important in human pathologies that are related to iron overload or deficiency.
T2  - Free Radical Biology and Medicine
T1  - Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.
VL  - 148
SP  - 123
EP  - 127
DO  - 10.1016/j.freeradbiomed.2020.01.001
ER  - 

@article{
author = "Korać Jačić, Jelena and Nikolić, Ljiljana and Stanković, Dalibor M. and Opačić, Miloš and Dimitrijević, Milena S. and Savić, Danijela and Grgurić-Šipka, Sanja and Spasojević, Ivan and Bogdanović-Pristov, Jelena",
year = "2020",
abstract = "Upon release in response to stress, epinephrine (Epi) may interact with labile iron pool in human plasma with potentially important (patho)physiological consequences. We have shown that Epi and Fe3+ build stable 1:1 high-spin bidentate complex at physiological pH, and that Epi does not undergo degradation in the presence of iron. However, the interactions of Epi with the more soluble Fe2+, and the impact of iron on biological activity of Epi are still not known. Herein we showed that Epi and Fe2+ build colorless complex which is stable under anaerobic conditions. In the presence of O2, Epi promoted the oxidation of Fe2+ and the formation of Epi-Fe3+ complex. Cyclic voltammetry showed that mid-point potential of Epi-Fe2+ complex is very low (-582 mV vs. standard hydrogen electrode), which explains catalyzed oxidation of Fe2+. Next, we examined the impact of iron binding on biological performance of Epi using patch clamping in cell culture with constitutive expression of adrenergic receptors. Epi alone evoked an increase of outward currents, whereas Epi in the complex with Fe3+ did not. This implies that the binding of Epi to adrenergic receptors and their activation is prevented by the formation of complex with iron. Pro-oxidative activity of Epi-Fe2+ complex may represent a link between chronic stress and cardiovascular problems. On the other hand, labile iron could serve as a modulator of biological activity of ligands. Such interactions may be important in human pathologies that are related to iron overload or deficiency.",
journal = "Free Radical Biology and Medicine",
title = "Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.",
volume = "148",
pages = "123-127",
doi = "10.1016/j.freeradbiomed.2020.01.001"
}

Korać Jačić, J., Nikolić, L., Stanković, D. M., Opačić, M., Dimitrijević, M. S., Savić, D., Grgurić-Šipka, S., Spasojević, I.,& Bogdanović-Pristov, J.. (2020). Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.. in Free Radical Biology and Medicine, 148, 123-127.
https://doi.org/10.1016/j.freeradbiomed.2020.01.001

Korać Jačić J, Nikolić L, Stanković DM, Opačić M, Dimitrijević MS, Savić D, Grgurić-Šipka S, Spasojević I, Bogdanović-Pristov J. Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.. in Free Radical Biology and Medicine. 2020;148:123-127.
doi:10.1016/j.freeradbiomed.2020.01.001 .

Korać Jačić, Jelena, Nikolić, Ljiljana, Stanković, Dalibor M., Opačić, Miloš, Dimitrijević, Milena S., Savić, Danijela, Grgurić-Šipka, Sanja, Spasojević, Ivan, Bogdanović-Pristov, Jelena, "Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors." in Free Radical Biology and Medicine, 148 (2020):123-127,
https://doi.org/10.1016/j.freeradbiomed.2020.01.001 . .

TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Petković, Branka
AU  - Pavković-Lučić, Sofija
AU  - Prolić, Zlatko M.
AU  - Anđelković, Marko
AU  - Savić, Tatjana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1540
AB  - Purpose: Extremely low frequency (ELF) magnetic fields as essential ecological factors may induce specific responses in genetically different lines. The object of this study was to investigate the impact of the ELF magnetic field on fitness components and locomotor activity of five Drosophila subobscura isofemale (IF) lines.Materials and methods: Each D. subobscura IF line, arbitrarily named: B16/1, B24/4, B39/1, B57/2 and B69/5, was maintained in five full-sib inbreeding generations. Their genetic structures were defined based on the mitochondrial DNA variability. Egg-first instar larvae and 1-day-old flies were exposed to an ELF magnetic field (50Hz, 0.5mT, 48h) and thereafter, fitness components and locomotor activity of males and females in an open field test were observed for each selected IF line, respectively.Results: Exposure of egg-first instar larvae to an ELF magnetic field shortened developmental time, and did not affect the viability and sex ratio of D. subobscura IF lines. Exposure of 1-day-old males and females IF lines B16/1 and B24/4 to an ELF magnetic field significantly decreased their locomotor activity and this effect lasted longer in females than males.Conclusions: These results indicate various responses of D. subobscura IF lines to the applied ELF magnetic field depending on their genetic background.
T2  - International Journal of Radiation Biology
T1  - Different responses of Drosophila subobscura isofemale lines to extremely low frequency magnetic field (50Hz, 0.5mT): fitness components and locomotor activity
VL  - 93
IS  - 5
SP  - 544
EP  - 552
DO  - 10.1080/09553002.2017.1268281
ER  - 

@article{
author = "Zmejkoski, Danica and Petković, Branka and Pavković-Lučić, Sofija and Prolić, Zlatko M. and Anđelković, Marko and Savić, Tatjana",
year = "2017",
abstract = "Purpose: Extremely low frequency (ELF) magnetic fields as essential ecological factors may induce specific responses in genetically different lines. The object of this study was to investigate the impact of the ELF magnetic field on fitness components and locomotor activity of five Drosophila subobscura isofemale (IF) lines.Materials and methods: Each D. subobscura IF line, arbitrarily named: B16/1, B24/4, B39/1, B57/2 and B69/5, was maintained in five full-sib inbreeding generations. Their genetic structures were defined based on the mitochondrial DNA variability. Egg-first instar larvae and 1-day-old flies were exposed to an ELF magnetic field (50Hz, 0.5mT, 48h) and thereafter, fitness components and locomotor activity of males and females in an open field test were observed for each selected IF line, respectively.Results: Exposure of egg-first instar larvae to an ELF magnetic field shortened developmental time, and did not affect the viability and sex ratio of D. subobscura IF lines. Exposure of 1-day-old males and females IF lines B16/1 and B24/4 to an ELF magnetic field significantly decreased their locomotor activity and this effect lasted longer in females than males.Conclusions: These results indicate various responses of D. subobscura IF lines to the applied ELF magnetic field depending on their genetic background.",
journal = "International Journal of Radiation Biology",
title = "Different responses of Drosophila subobscura isofemale lines to extremely low frequency magnetic field (50Hz, 0.5mT): fitness components and locomotor activity",
volume = "93",
number = "5",
pages = "544-552",
doi = "10.1080/09553002.2017.1268281"
}

Zmejkoski, D., Petković, B., Pavković-Lučić, S., Prolić, Z. M., Anđelković, M.,& Savić, T.. (2017). Different responses of Drosophila subobscura isofemale lines to extremely low frequency magnetic field (50Hz, 0.5mT): fitness components and locomotor activity. in International Journal of Radiation Biology, 93(5), 544-552.
https://doi.org/10.1080/09553002.2017.1268281

Zmejkoski D, Petković B, Pavković-Lučić S, Prolić ZM, Anđelković M, Savić T. Different responses of Drosophila subobscura isofemale lines to extremely low frequency magnetic field (50Hz, 0.5mT): fitness components and locomotor activity. in International Journal of Radiation Biology. 2017;93(5):544-552.
doi:10.1080/09553002.2017.1268281 .

Zmejkoski, Danica, Petković, Branka, Pavković-Lučić, Sofija, Prolić, Zlatko M., Anđelković, Marko, Savić, Tatjana, "Different responses of Drosophila subobscura isofemale lines to extremely low frequency magnetic field (50Hz, 0.5mT): fitness components and locomotor activity" in International Journal of Radiation Biology, 93, no. 5 (2017):544-552,
https://doi.org/10.1080/09553002.2017.1268281 . .

TY  - JOUR
AU  - Peric-Mataruga, Vesna
AU  - Petković, Branka
AU  - Ilijin, Larisa
AU  - Mrdakovic, Marija
AU  - Dronjak, Slađana
AU  - Todorović, Dajana
AU  - Vlahovic, Milena
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1705
AB  - Insects brain as a part of nervous system is the first-line of fast stress response that integrate stress signals to regulate all aspects of insect physiology and behaviour. The cadmium (Cd) bioaccumulation factor (BF), activity of the neurotoxicity biomarker acetylcholinesterase (AChE), dopamine content, expression and amount of Hsp70 in the brain and locomotor activity were evaluated in the 4th instar of Lymantria dispar L caterpillars fed a Cd supplemented diet and reared in an optimal temperature regime (23 degrees C) and/or exposed to high temperature (28 degrees C). The insects originated from two forests, one close to Nikola Tesla thermoelectric power plant, Obrenovac (polluted population), and the other Kosmaj mountain (less-polluted population, far from any industrial region). The Cd BF was higher in the less polluted than in the polluted population especially at the high ambient temperature. AChE activity and dopamine content were changed in the brains of L. dispar from both populations in the same manner. Hsp70 concentration in caterpillar brains showed opposite trends, a decrease in the less polluted and an increase in the polluted population. Locomotor activity was modified in both Lymantria dispar populations, but the pattern of changes depended on the stressors and their combined effect. ACh activity and dopamine content are sensitive parameters to Cd exposure, regardless of pollutant experience, and might be promising biomarkers in monitoring forest ecosystems. (C) 2017 Elsevier Ltd. All rights reserved.
T2  - Chemosphere
T1  - Cadmium and high temperature effects on brain and behaviour of Lymantria dispar L. caterpillars originating from polluted and less-polluted forests
VL  - 185
SP  - 628
EP  - 636
DO  - 10.1016/j.chemosphere.2017.07.050
ER  - 

@article{
author = "Peric-Mataruga, Vesna and Petković, Branka and Ilijin, Larisa and Mrdakovic, Marija and Dronjak, Slađana and Todorović, Dajana and Vlahovic, Milena",
year = "2017",
abstract = "Insects brain as a part of nervous system is the first-line of fast stress response that integrate stress signals to regulate all aspects of insect physiology and behaviour. The cadmium (Cd) bioaccumulation factor (BF), activity of the neurotoxicity biomarker acetylcholinesterase (AChE), dopamine content, expression and amount of Hsp70 in the brain and locomotor activity were evaluated in the 4th instar of Lymantria dispar L caterpillars fed a Cd supplemented diet and reared in an optimal temperature regime (23 degrees C) and/or exposed to high temperature (28 degrees C). The insects originated from two forests, one close to Nikola Tesla thermoelectric power plant, Obrenovac (polluted population), and the other Kosmaj mountain (less-polluted population, far from any industrial region). The Cd BF was higher in the less polluted than in the polluted population especially at the high ambient temperature. AChE activity and dopamine content were changed in the brains of L. dispar from both populations in the same manner. Hsp70 concentration in caterpillar brains showed opposite trends, a decrease in the less polluted and an increase in the polluted population. Locomotor activity was modified in both Lymantria dispar populations, but the pattern of changes depended on the stressors and their combined effect. ACh activity and dopamine content are sensitive parameters to Cd exposure, regardless of pollutant experience, and might be promising biomarkers in monitoring forest ecosystems. (C) 2017 Elsevier Ltd. All rights reserved.",
journal = "Chemosphere",
title = "Cadmium and high temperature effects on brain and behaviour of Lymantria dispar L. caterpillars originating from polluted and less-polluted forests",
volume = "185",
pages = "628-636",
doi = "10.1016/j.chemosphere.2017.07.050"
}

Peric-Mataruga, V., Petković, B., Ilijin, L., Mrdakovic, M., Dronjak, S., Todorović, D.,& Vlahovic, M.. (2017). Cadmium and high temperature effects on brain and behaviour of Lymantria dispar L. caterpillars originating from polluted and less-polluted forests. in Chemosphere, 185, 628-636.
https://doi.org/10.1016/j.chemosphere.2017.07.050

Peric-Mataruga V, Petković B, Ilijin L, Mrdakovic M, Dronjak S, Todorović D, Vlahovic M. Cadmium and high temperature effects on brain and behaviour of Lymantria dispar L. caterpillars originating from polluted and less-polluted forests. in Chemosphere. 2017;185:628-636.
doi:10.1016/j.chemosphere.2017.07.050 .

Peric-Mataruga, Vesna, Petković, Branka, Ilijin, Larisa, Mrdakovic, Marija, Dronjak, Slađana, Todorović, Dajana, Vlahovic, Milena, "Cadmium and high temperature effects on brain and behaviour of Lymantria dispar L. caterpillars originating from polluted and less-polluted forests" in Chemosphere, 185 (2017):628-636,
https://doi.org/10.1016/j.chemosphere.2017.07.050 . .

TY  - JOUR
AU  - Dimitrijevic, Danica
AU  - Savić, Tatjana
AU  - Anđelković, Marko
AU  - Prolić, Zlatko M.
AU  - Janac, Branka
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5985
AB  - Purpose: Extremely low frequency (ELF) magnetic fields are essential ecological factors which may induce changes in many organisms. The aim of this study was to examine the effects in Drosophila subobscura exposed for 48 h to ELF magnetic field (50 Hz, 0.5 mT) at different developmental stages. Materials and methods: Egg-first instar larvae developmental stage of D. subobscura isofemale lines was exposed to ELF magnetic field, and fitness components (developmental time, developmental dynamics, viability and sex ratio) and locomotor activity of three-day-old males and females were monitored. Also, just eclosed D. subobscura isofemale adults were exposed to ELF magnetic field and their locomotor activity was monitored just after. Results: ELF magnetic field shortens developmental time, increases viability and does not affect sex ratio of D. subobscura. No matter which developmental stage is exposed, ELF magnetic field significantly decreases locomotor activity of adult flies, but after exposure of just eclosed adults observed change lasts longer. Conclusions: Applied ELF magnetic field modifies fitness components and locomotor activity of D. subobscura. Observed effects can be attributed to the influence of magnetic field on different stages of development where the hormonal and nervous systems play important role in the control of examined parameters.
T2  - International Journal of Radiation Biology
T1  - Extremely low frequency magnetic field (50 Hz, 0.5 mT) modifies fitness components and locomotor activity of Drosophila subobscura
VL  - 90
IS  - 5
SP  - 337
EP  - 343
DO  - 10.3109/09553002.2014.888105
ER  - 

@article{
author = "Dimitrijevic, Danica and Savić, Tatjana and Anđelković, Marko and Prolić, Zlatko M. and Janac, Branka",
year = "2014",
abstract = "Purpose: Extremely low frequency (ELF) magnetic fields are essential ecological factors which may induce changes in many organisms. The aim of this study was to examine the effects in Drosophila subobscura exposed for 48 h to ELF magnetic field (50 Hz, 0.5 mT) at different developmental stages. Materials and methods: Egg-first instar larvae developmental stage of D. subobscura isofemale lines was exposed to ELF magnetic field, and fitness components (developmental time, developmental dynamics, viability and sex ratio) and locomotor activity of three-day-old males and females were monitored. Also, just eclosed D. subobscura isofemale adults were exposed to ELF magnetic field and their locomotor activity was monitored just after. Results: ELF magnetic field shortens developmental time, increases viability and does not affect sex ratio of D. subobscura. No matter which developmental stage is exposed, ELF magnetic field significantly decreases locomotor activity of adult flies, but after exposure of just eclosed adults observed change lasts longer. Conclusions: Applied ELF magnetic field modifies fitness components and locomotor activity of D. subobscura. Observed effects can be attributed to the influence of magnetic field on different stages of development where the hormonal and nervous systems play important role in the control of examined parameters.",
journal = "International Journal of Radiation Biology",
title = "Extremely low frequency magnetic field (50 Hz, 0.5 mT) modifies fitness components and locomotor activity of Drosophila subobscura",
volume = "90",
number = "5",
pages = "337-343",
doi = "10.3109/09553002.2014.888105"
}

Dimitrijevic, D., Savić, T., Anđelković, M., Prolić, Z. M.,& Janac, B.. (2014). Extremely low frequency magnetic field (50 Hz, 0.5 mT) modifies fitness components and locomotor activity of Drosophila subobscura. in International Journal of Radiation Biology, 90(5), 337-343.
https://doi.org/10.3109/09553002.2014.888105

Dimitrijevic D, Savić T, Anđelković M, Prolić ZM, Janac B. Extremely low frequency magnetic field (50 Hz, 0.5 mT) modifies fitness components and locomotor activity of Drosophila subobscura. in International Journal of Radiation Biology. 2014;90(5):337-343.
doi:10.3109/09553002.2014.888105 .

Dimitrijevic, Danica, Savić, Tatjana, Anđelković, Marko, Prolić, Zlatko M., Janac, Branka, "Extremely low frequency magnetic field (50 Hz, 0.5 mT) modifies fitness components and locomotor activity of Drosophila subobscura" in International Journal of Radiation Biology, 90, no. 5 (2014):337-343,
https://doi.org/10.3109/09553002.2014.888105 . .