TY  - JOUR
AU  - Miljković, Miona G.
AU  - Lazić, Vesna M.
AU  - Davidović, Slađana Z.
AU  - Milivojević, Ana
AU  - Papan, Jelena
AU  - Fernandes, Margarida M.
AU  - Lanceros-Mendez, Senentxu
AU  - Ahrenkiel, Scott Phillip
AU  - Nedeljković, Jovan
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8902
AB  - The aim of this study was the development of a non-toxic, biosynthetic antimicrobial agent which selectively acts on only one type of microorganism, and preserves the microbiota. Antimicrobial performance of biosynthesized silver nanoparticles (Ag NPs) by horsetail (Equisetum arvense L.) extract was examined against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus, as well as yeasts Candida albicans and Saccharomyces boulardii. Also, the cytotoxicity of Ag NPs was examined toward pre-osteoblast cells. The synthetic conditions—concentration of extract, temperature, and pH—were optimized to prepare silver colloids with different particle size distributions and long-term stability. The obtained samples were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The smaller-sized Ag NPs (~ 10–20 nm), prepared at a lower temperature (20 °C), showed better antimicrobial performance against E. coli compared to larger ones (~ 40–60 nm), prepared at high temperature (100 °C). On the other hand, both samples did not display any toxic action against bacteria S. aureus, or yeasts C. albicans and S. boulardii. Non-cytotoxic behavior of Ag NPs toward pre-osteoblast cells was observed for the concentrations of silver ≤ 2.25 and ≤ 4.5 mg L−1 for 10–20 and 40–60 nm-sized Ag NPs, respectively. Biosynthesized Ag NPs by horsetail extract display selective toxic action against E. coli at the ecologically acceptable concentration level. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Journal of Inorganic and Organometallic Polymers and Materials
T1  - Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli
VL  - 30
IS  - 7
SP  - 2598
EP  - 2607
DO  - 10.1007/s10904-019-01402-x
ER  - 

@article{
author = "Miljković, Miona G. and Lazić, Vesna M. and Davidović, Slađana Z. and Milivojević, Ana and Papan, Jelena and Fernandes, Margarida M. and Lanceros-Mendez, Senentxu and Ahrenkiel, Scott Phillip and Nedeljković, Jovan",
year = "2020",
abstract = "The aim of this study was the development of a non-toxic, biosynthetic antimicrobial agent which selectively acts on only one type of microorganism, and preserves the microbiota. Antimicrobial performance of biosynthesized silver nanoparticles (Ag NPs) by horsetail (Equisetum arvense L.) extract was examined against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus, as well as yeasts Candida albicans and Saccharomyces boulardii. Also, the cytotoxicity of Ag NPs was examined toward pre-osteoblast cells. The synthetic conditions—concentration of extract, temperature, and pH—were optimized to prepare silver colloids with different particle size distributions and long-term stability. The obtained samples were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The smaller-sized Ag NPs (~ 10–20 nm), prepared at a lower temperature (20 °C), showed better antimicrobial performance against E. coli compared to larger ones (~ 40–60 nm), prepared at high temperature (100 °C). On the other hand, both samples did not display any toxic action against bacteria S. aureus, or yeasts C. albicans and S. boulardii. Non-cytotoxic behavior of Ag NPs toward pre-osteoblast cells was observed for the concentrations of silver ≤ 2.25 and ≤ 4.5 mg L−1 for 10–20 and 40–60 nm-sized Ag NPs, respectively. Biosynthesized Ag NPs by horsetail extract display selective toxic action against E. coli at the ecologically acceptable concentration level. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Journal of Inorganic and Organometallic Polymers and Materials",
title = "Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli",
volume = "30",
number = "7",
pages = "2598-2607",
doi = "10.1007/s10904-019-01402-x"
}

Miljković, M. G., Lazić, V. M., Davidović, S. Z., Milivojević, A., Papan, J., Fernandes, M. M., Lanceros-Mendez, S., Ahrenkiel, S. P.,& Nedeljković, J.. (2020). Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli. in Journal of Inorganic and Organometallic Polymers and Materials, 30(7), 2598-2607.
https://doi.org/10.1007/s10904-019-01402-x

Miljković MG, Lazić VM, Davidović SZ, Milivojević A, Papan J, Fernandes MM, Lanceros-Mendez S, Ahrenkiel SP, Nedeljković J. Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli. in Journal of Inorganic and Organometallic Polymers and Materials. 2020;30(7):2598-2607.
doi:10.1007/s10904-019-01402-x .

Miljković, Miona G., Lazić, Vesna M., Davidović, Slađana Z., Milivojević, Ana, Papan, Jelena, Fernandes, Margarida M., Lanceros-Mendez, Senentxu, Ahrenkiel, Scott Phillip, Nedeljković, Jovan, "Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli" in Journal of Inorganic and Organometallic Polymers and Materials, 30, no. 7 (2020):2598-2607,
https://doi.org/10.1007/s10904-019-01402-x . .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
AU  - Dimitrijević-Branković, Suzana I.
AU  - Davidović, Slađana Z.
AU  - Radovanović, Neda
AU  - Delattre, Cédric
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8800
AB  - The use of polysaccharide-based materials presents an eco-friendly technological solution, by reducing dependence on fossil resources while reducing a product’s carbon footprint, when compared to conventional plastic packaging materials. This review discusses the potential of polysaccharides as a raw material to produce multifunctional materials for food packaging applications. The covered areas include the recent innovations and properties of the polysaccharide-based materials. Emphasis is given to hemicelluloses, marine polysaccharides, and bacterial exopolysaccharides and their potential application in the latest trends of food packaging materials, including edible coatings, intelligent films, and thermo-insulated aerogel packaging.
T2  - Molecules
T1  - Prospect of Polysaccharide-Based Materials as Advanced Food Packaging
VL  - 25
IS  - 1
SP  - 135
DO  - 10.3390/molecules25010135
ER  - 

@article{
author = "Nešić, Aleksandra and Cabrera-Barjas, Gustavo and Dimitrijević-Branković, Suzana I. and Davidović, Slađana Z. and Radovanović, Neda and Delattre, Cédric",
year = "2019",
abstract = "The use of polysaccharide-based materials presents an eco-friendly technological solution, by reducing dependence on fossil resources while reducing a product’s carbon footprint, when compared to conventional plastic packaging materials. This review discusses the potential of polysaccharides as a raw material to produce multifunctional materials for food packaging applications. The covered areas include the recent innovations and properties of the polysaccharide-based materials. Emphasis is given to hemicelluloses, marine polysaccharides, and bacterial exopolysaccharides and their potential application in the latest trends of food packaging materials, including edible coatings, intelligent films, and thermo-insulated aerogel packaging.",
journal = "Molecules",
title = "Prospect of Polysaccharide-Based Materials as Advanced Food Packaging",
volume = "25",
number = "1",
pages = "135",
doi = "10.3390/molecules25010135"
}

Nešić, A., Cabrera-Barjas, G., Dimitrijević-Branković, S. I., Davidović, S. Z., Radovanović, N.,& Delattre, C.. (2019). Prospect of Polysaccharide-Based Materials as Advanced Food Packaging. in Molecules, 25(1), 135.
https://doi.org/10.3390/molecules25010135

Nešić A, Cabrera-Barjas G, Dimitrijević-Branković SI, Davidović SZ, Radovanović N, Delattre C. Prospect of Polysaccharide-Based Materials as Advanced Food Packaging. in Molecules. 2019;25(1):135.
doi:10.3390/molecules25010135 .

Nešić, Aleksandra, Cabrera-Barjas, Gustavo, Dimitrijević-Branković, Suzana I., Davidović, Slađana Z., Radovanović, Neda, Delattre, Cédric, "Prospect of Polysaccharide-Based Materials as Advanced Food Packaging" in Molecules, 25, no. 1 (2019):135,
https://doi.org/10.3390/molecules25010135 . .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Gordić, Milan V.
AU  - Petrović, Jelena M.
AU  - Pavlović, Vladimir B.
AU  - Mitrić, Miodrag
AU  - Nešić, Aleksandra
AU  - Dimitrijević-Branković, Suzana I.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8454
AB  - New agar-based composite films with increasing Cu-carbonate and Cu-phosphate mineral phase content were prepared by in situ mineralization and solvent casting method. SEM and optical analysis revealed that Cu-carbonate phase had better compatibility with agar matrix than Cu-phosphate phase. Incorporation of both mineral phases improved mechanical and water vapor barrier properties of the obtained mineralized films, in concentration dependent manner. When 5 mM of carbonate precursor was incorporated into agar matrix, mechanical resistance was enchanced for 44% and water vapor barrier property for 40%. The release of Cu (II) was higher in acidic conditions for both mineralized composites and remained in the range of specific release limits for this metal. In addition, both mineralized composite films exhibited distinctive antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Overall, the Cu-carbonate and Cu-phosphate mineralized agar films showed potential to be used for food packaging materials, agriculture or medical purposes. © 2019 Elsevier Ltd
T2  - European Polymer Journal
T1  - Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase
VL  - 119
SP  - 352
EP  - 358
DO  - 10.1016/j.eurpolymj.2019.08.004
ER  - 

@article{
author = "Radovanović, Neda and Malagurski, Ivana and Lević, Steva and Gordić, Milan V. and Petrović, Jelena M. and Pavlović, Vladimir B. and Mitrić, Miodrag and Nešić, Aleksandra and Dimitrijević-Branković, Suzana I.",
year = "2019",
abstract = "New agar-based composite films with increasing Cu-carbonate and Cu-phosphate mineral phase content were prepared by in situ mineralization and solvent casting method. SEM and optical analysis revealed that Cu-carbonate phase had better compatibility with agar matrix than Cu-phosphate phase. Incorporation of both mineral phases improved mechanical and water vapor barrier properties of the obtained mineralized films, in concentration dependent manner. When 5 mM of carbonate precursor was incorporated into agar matrix, mechanical resistance was enchanced for 44% and water vapor barrier property for 40%. The release of Cu (II) was higher in acidic conditions for both mineralized composites and remained in the range of specific release limits for this metal. In addition, both mineralized composite films exhibited distinctive antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Overall, the Cu-carbonate and Cu-phosphate mineralized agar films showed potential to be used for food packaging materials, agriculture or medical purposes. © 2019 Elsevier Ltd",
journal = "European Polymer Journal",
title = "Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase",
volume = "119",
pages = "352-358",
doi = "10.1016/j.eurpolymj.2019.08.004"
}

Radovanović, N., Malagurski, I., Lević, S., Gordić, M. V., Petrović, J. M., Pavlović, V. B., Mitrić, M., Nešić, A.,& Dimitrijević-Branković, S. I.. (2019). Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase. in European Polymer Journal, 119, 352-358.
https://doi.org/10.1016/j.eurpolymj.2019.08.004

Radovanović N, Malagurski I, Lević S, Gordić MV, Petrović JM, Pavlović VB, Mitrić M, Nešić A, Dimitrijević-Branković SI. Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase. in European Polymer Journal. 2019;119:352-358.
doi:10.1016/j.eurpolymj.2019.08.004 .

Radovanović, Neda, Malagurski, Ivana, Lević, Steva, Gordić, Milan V., Petrović, Jelena M., Pavlović, Vladimir B., Mitrić, Miodrag, Nešić, Aleksandra, Dimitrijević-Branković, Suzana I., "Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase" in European Polymer Journal, 119 (2019):352-358,
https://doi.org/10.1016/j.eurpolymj.2019.08.004 . .

TY  - JOUR
AU  - Davidović, Slađana Z.
AU  - Lazić, Vesna M.
AU  - Miljković, Miona G.
AU  - Gordić, Milan V.
AU  - Sekulić, Milica
AU  - Marinović-Cincović, Milena
AU  - Ratnayake, Ishara S.
AU  - Ahrenkiel, Scott Phillip
AU  - Nedeljković, Jovan
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8452
AB  - The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (<2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10–20 nm silver nanoparticles (Ag NPs) within the agar-agar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agar-agar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inorganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureuswas observed for all agar-silver films, while satisfactory results were observed for Gram-negative bacteria Pseudomonas aeruginosa (≥99.6%). The release of Ag+ ions is suppressed by the increase of the concentration of Mg2+ ions and it was found to be significantly smaller (≤0.24 ppm) than the harmful ecological level (1 ppm). © 2019 Elsevier Ltd
T2  - Carbohydrate Polymers
T1  - Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions
VL  - 224
SP  - 115187
DO  - 10.1016/j.carbpol.2019.115187
ER  - 

@article{
author = "Davidović, Slađana Z. and Lazić, Vesna M. and Miljković, Miona G. and Gordić, Milan V. and Sekulić, Milica and Marinović-Cincović, Milena and Ratnayake, Ishara S. and Ahrenkiel, Scott Phillip and Nedeljković, Jovan",
year = "2019",
abstract = "The antibacterial ability of in situ prepared nanometer-sized silver particles, immobilized in agar-agar films, was studied as a function of the concentration of co-dopant, magnesium ions. Content of inorganic components in hybrid films was determined using inductively coupled plasma optic emission spectroscopy, and found to be low (<2 wt.-%). Morphology of prepared hybrid films, studied by transmission electron microscopy, revealed the presence of non-agglomerated and randomly distributed 10–20 nm silver nanoparticles (Ag NPs) within the agar-agar matrices. Fourier-transform infrared spectroscopy indicated the distinct chemical interaction between Ag NPs and polymer chains. Thermogravimetric analysis, as well as the determination of tensile strength, Young's modulus, and elongation at break showed improvement of thermal stability and mechanical properties of agar-agar matrices upon the incorporation of Ag NPs due to high compatibility between the hydrophilic organic component and inorganic components. The complete microbial reduction of Gram-positive bacteria Staphylococcus aureuswas observed for all agar-silver films, while satisfactory results were observed for Gram-negative bacteria Pseudomonas aeruginosa (≥99.6%). The release of Ag+ ions is suppressed by the increase of the concentration of Mg2+ ions and it was found to be significantly smaller (≤0.24 ppm) than the harmful ecological level (1 ppm). © 2019 Elsevier Ltd",
journal = "Carbohydrate Polymers",
title = "Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions",
volume = "224",
pages = "115187",
doi = "10.1016/j.carbpol.2019.115187"
}

Davidović, S. Z., Lazić, V. M., Miljković, M. G., Gordić, M. V., Sekulić, M., Marinović-Cincović, M., Ratnayake, I. S., Ahrenkiel, S. P.,& Nedeljković, J.. (2019). Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions. in Carbohydrate Polymers, 224, 115187.
https://doi.org/10.1016/j.carbpol.2019.115187

Davidović SZ, Lazić VM, Miljković MG, Gordić MV, Sekulić M, Marinović-Cincović M, Ratnayake IS, Ahrenkiel SP, Nedeljković J. Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions. in Carbohydrate Polymers. 2019;224:115187.
doi:10.1016/j.carbpol.2019.115187 .

Davidović, Slađana Z., Lazić, Vesna M., Miljković, Miona G., Gordić, Milan V., Sekulić, Milica, Marinović-Cincović, Milena, Ratnayake, Ishara S., Ahrenkiel, Scott Phillip, Nedeljković, Jovan, "Antibacterial ability of immobilized silver nanoparticles in agar-agar films co-doped with magnesium ions" in Carbohydrate Polymers, 224 (2019):115187,
https://doi.org/10.1016/j.carbpol.2019.115187 . .

TY  - CONF
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Radovanović, Neda
AU  - Gordić, Milan V.
AU  - Carević, Milica
AU  - Nešić, Aleksandra
AU  - Dimitrijević, Suzana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10723
AB  - Dextransucrase (DS), the extracellular enzyme is of immense industrial importance, due to ability to produce dextran and oligosaccharides (OS). Worldwide interest in OS has been increasing, since they have been accorded the prebiotic status. However, the industrial application of DS for OS synthesis is limited, due to low yield of enzyme production and its low catalytic activity. Hence, there is a great interest for development of new technologies that can provide improved performance of biocatalyst. Enzyme immobilization technology is considered to be a crucial step for cheaper and more efficient usage of DS. Entrapment is one of the widely investigated immobilization methods, where enzymes are enclosed or confined within the polymer matrix without altering their native structure, developing bioreactors for commercial applications. Different matrices such as polyacryl-amide gel, alginate beads and agar–agar have been used for the entrapment of different enzymes and among them agar–agar is a biocompatible, non-toxic and strong solidifying agent for immobilization of various enzymes. In this work, the entrapment of DS was initiated by different quantity (1:9, 1:4 and 1:1) of dialyzed enzyme into agar- agar solution. Agar solution was prepared in distilled water by vigorous shaking at 100°C, autoclaved and was allowed to cool to 40–45°C. Afterwards, enzyme was incorporated and mixed thoroughly. This mixture was immediately poured into a clean Petri plate and left to solidify at room temperature. Polymer films with and without immobilized DS were analyzed in terms of enzyme activity and reusability and mechanical properties (tensile strength, elongation at break and elastic modulus). In order to remove un-entrapped enzyme the films were washed with double deionized water and sodium acetate buffer (pH 5.4) three times prior to enzymatic assay. For quantitative analysis of samples for OS production a Dionex Ultimate 3000 HPLC system was used. Results showed that maximum immobilization yield (74.11%) was achieved by use of 2 % agar and (1:9) enzyme: agar ratio. HPLC analysis confirmed the similar trend of OS formation in immobilized samples compared to free enzyme. The lowest tested fraction of enzyme immobilized into polymer matrix (1:9) improved tensile strength of films in comparison with control film, whereas higher concentration of enzyme led to decrease in mechanical resistance of films. Scanning electron microscopy of agar films before and after DS entrapment revealed significant morphological change on the matrix surface. Considering the economic feasibility, the entrapped DS indicated imperative recycling efficiency up to six reaction cycles. The results of this study revealed that an easily available and inexpensive matrix could be successfully employed for DS immobilization and OS production.
C3  - PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia
T1  - Dextransucrase entrapment as an efficient alternative for increased recycling efficiency of free enzyme within agar-agar film matrix
SP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10723
ER  - 

@conference{
author = "Miljković, Miona and Davidović, Slađana and Radovanović, Neda and Gordić, Milan V. and Carević, Milica and Nešić, Aleksandra and Dimitrijević, Suzana",
year = "2018",
abstract = "Dextransucrase (DS), the extracellular enzyme is of immense industrial importance, due to ability to produce dextran and oligosaccharides (OS). Worldwide interest in OS has been increasing, since they have been accorded the prebiotic status. However, the industrial application of DS for OS synthesis is limited, due to low yield of enzyme production and its low catalytic activity. Hence, there is a great interest for development of new technologies that can provide improved performance of biocatalyst. Enzyme immobilization technology is considered to be a crucial step for cheaper and more efficient usage of DS. Entrapment is one of the widely investigated immobilization methods, where enzymes are enclosed or confined within the polymer matrix without altering their native structure, developing bioreactors for commercial applications. Different matrices such as polyacryl-amide gel, alginate beads and agar–agar have been used for the entrapment of different enzymes and among them agar–agar is a biocompatible, non-toxic and strong solidifying agent for immobilization of various enzymes. In this work, the entrapment of DS was initiated by different quantity (1:9, 1:4 and 1:1) of dialyzed enzyme into agar- agar solution. Agar solution was prepared in distilled water by vigorous shaking at 100°C, autoclaved and was allowed to cool to 40–45°C. Afterwards, enzyme was incorporated and mixed thoroughly. This mixture was immediately poured into a clean Petri plate and left to solidify at room temperature. Polymer films with and without immobilized DS were analyzed in terms of enzyme activity and reusability and mechanical properties (tensile strength, elongation at break and elastic modulus). In order to remove un-entrapped enzyme the films were washed with double deionized water and sodium acetate buffer (pH 5.4) three times prior to enzymatic assay. For quantitative analysis of samples for OS production a Dionex Ultimate 3000 HPLC system was used. Results showed that maximum immobilization yield (74.11%) was achieved by use of 2 % agar and (1:9) enzyme: agar ratio. HPLC analysis confirmed the similar trend of OS formation in immobilized samples compared to free enzyme. The lowest tested fraction of enzyme immobilized into polymer matrix (1:9) improved tensile strength of films in comparison with control film, whereas higher concentration of enzyme led to decrease in mechanical resistance of films. Scanning electron microscopy of agar films before and after DS entrapment revealed significant morphological change on the matrix surface. Considering the economic feasibility, the entrapped DS indicated imperative recycling efficiency up to six reaction cycles. The results of this study revealed that an easily available and inexpensive matrix could be successfully employed for DS immobilization and OS production.",
journal = "PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia",
title = "Dextransucrase entrapment as an efficient alternative for increased recycling efficiency of free enzyme within agar-agar film matrix",
pages = "88",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10723"
}

Miljković, M., Davidović, S., Radovanović, N., Gordić, M. V., Carević, M., Nešić, A.,& Dimitrijević, S.. (2018). Dextransucrase entrapment as an efficient alternative for increased recycling efficiency of free enzyme within agar-agar film matrix. in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia, 88.
https://hdl.handle.net/21.15107/rcub_vinar_10723

Miljković M, Davidović S, Radovanović N, Gordić MV, Carević M, Nešić A, Dimitrijević S. Dextransucrase entrapment as an efficient alternative for increased recycling efficiency of free enzyme within agar-agar film matrix. in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia. 2018;:88.
https://hdl.handle.net/21.15107/rcub_vinar_10723 .

Miljković, Miona, Davidović, Slađana, Radovanović, Neda, Gordić, Milan V., Carević, Milica, Nešić, Aleksandra, Dimitrijević, Suzana, "Dextransucrase entrapment as an efficient alternative for increased recycling efficiency of free enzyme within agar-agar film matrix" in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia (2018):88,
https://hdl.handle.net/21.15107/rcub_vinar_10723 .

TY  - JOUR
AU  - Miljković, Miona G.
AU  - Lazić, Vesna M.
AU  - Banjanac, Katarina
AU  - Davidović, Slađana Z.
AU  - Bezbradica, Dejan I.
AU  - Marinković, Aleksandar D.
AU  - Sredojević, Dušan
AU  - Nedeljković, Jovan
AU  - Dimitrijević-Branković, Suzana I.
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0141813018302952
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7776
AB  - The TiO2 based hybrid supports with different functional groups (amino, glutaraldehyde or epoxy) were prepared and their influence on immobilization of dextransucrase (DS) was studied. Novel synthetic route for surface modification of TiO2 with amino and glutaraldehyde groups was developed taking advantage of charge transfer complex (CTC) formation between surface Ti atoms and salicylate-type of ligand (5 aminosalicylic acid (5-ASA)). The proposed coordination of 5-ASA to the surface of TiO2 powder and optical properties of CTC was presented. The pristine TiO2 and amino functionalized TiO2 have higher sorption capacity for DS (12.6 and 12.0 mg g(-1), respectively) compared to glutaraldehyde and epoxy activated supports (9.6 and 9.8 mg g(-1) respectively). However, immobilized enzyme to either glutaraldehyde or epoxy functionalized TiO2 have almost two times higher expressed activities compared to pristine TiO2 support (258, 235 and 142 IU g(-1), respectively). Thermal stability of enzyme immobilized on glutaraldehyde and epoxy functionalized supports was studied at 40 degrees C, as well as operational stability under long-run working conditions in repeated cycles. After five cycles, DS imobilized on glutaraldehyde activated support retained almost 70% of its initial expressed activity, while, after five cycles, performance of DS immobilized on epoxy activated support was significantly lower (15%).
T2  - International Journal of Biological Macromolecules
T1  - Immobilization of dextransucrase on functionalized TiO2 supports
VL  - 114
SP  - 1216
EP  - 1223
DO  - 10.1016/j.ijbiomac.2018.04.027
ER  - 

@article{
author = "Miljković, Miona G. and Lazić, Vesna M. and Banjanac, Katarina and Davidović, Slađana Z. and Bezbradica, Dejan I. and Marinković, Aleksandar D. and Sredojević, Dušan and Nedeljković, Jovan and Dimitrijević-Branković, Suzana I.",
year = "2018",
abstract = "The TiO2 based hybrid supports with different functional groups (amino, glutaraldehyde or epoxy) were prepared and their influence on immobilization of dextransucrase (DS) was studied. Novel synthetic route for surface modification of TiO2 with amino and glutaraldehyde groups was developed taking advantage of charge transfer complex (CTC) formation between surface Ti atoms and salicylate-type of ligand (5 aminosalicylic acid (5-ASA)). The proposed coordination of 5-ASA to the surface of TiO2 powder and optical properties of CTC was presented. The pristine TiO2 and amino functionalized TiO2 have higher sorption capacity for DS (12.6 and 12.0 mg g(-1), respectively) compared to glutaraldehyde and epoxy activated supports (9.6 and 9.8 mg g(-1) respectively). However, immobilized enzyme to either glutaraldehyde or epoxy functionalized TiO2 have almost two times higher expressed activities compared to pristine TiO2 support (258, 235 and 142 IU g(-1), respectively). Thermal stability of enzyme immobilized on glutaraldehyde and epoxy functionalized supports was studied at 40 degrees C, as well as operational stability under long-run working conditions in repeated cycles. After five cycles, DS imobilized on glutaraldehyde activated support retained almost 70% of its initial expressed activity, while, after five cycles, performance of DS immobilized on epoxy activated support was significantly lower (15%).",
journal = "International Journal of Biological Macromolecules",
title = "Immobilization of dextransucrase on functionalized TiO2 supports",
volume = "114",
pages = "1216-1223",
doi = "10.1016/j.ijbiomac.2018.04.027"
}

Miljković, M. G., Lazić, V. M., Banjanac, K., Davidović, S. Z., Bezbradica, D. I., Marinković, A. D., Sredojević, D., Nedeljković, J.,& Dimitrijević-Branković, S. I.. (2018). Immobilization of dextransucrase on functionalized TiO2 supports. in International Journal of Biological Macromolecules, 114, 1216-1223.
https://doi.org/10.1016/j.ijbiomac.2018.04.027

Miljković MG, Lazić VM, Banjanac K, Davidović SZ, Bezbradica DI, Marinković AD, Sredojević D, Nedeljković J, Dimitrijević-Branković SI. Immobilization of dextransucrase on functionalized TiO2 supports. in International Journal of Biological Macromolecules. 2018;114:1216-1223.
doi:10.1016/j.ijbiomac.2018.04.027 .

Miljković, Miona G., Lazić, Vesna M., Banjanac, Katarina, Davidović, Slađana Z., Bezbradica, Dejan I., Marinković, Aleksandar D., Sredojević, Dušan, Nedeljković, Jovan, Dimitrijević-Branković, Suzana I., "Immobilization of dextransucrase on functionalized TiO2 supports" in International Journal of Biological Macromolecules, 114 (2018):1216-1223,
https://doi.org/10.1016/j.ijbiomac.2018.04.027 . .

TY  - JOUR
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Mitrić, Miodrag
AU  - Pavlović, Vladimir B.
AU  - Dimitrijević-Branković, Suzana I.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1817
AB  - Two bimetallic (Zn/Cu) alginate based nanocomposites, impregnated with carbonate or phosphate mineral phase, were prepared by a facile procedure. Mineralized samples exhibited different morphologies and properties when compared to the non-mineralized sample. Antimicrobial testing against Escherichia coil, Staphylococcus aureus and Candida albicans showed that mineralized samples are more efficient than non-mineralized in elimination of microorganisms. The results of this study suggest that bimetallic mineralized alginates could be potentially used as affordable, easy to produce antimicrobial materials. (C) 2017 Elsevier By. All rights reserved.
T2  - Materials Letters
T1  - Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application
VL  - 212
SP  - 32
EP  - 36
DO  - 10.1016/j.matlet.2017.10.046
ER  - 

@article{
author = "Malagurski, Ivana and Lević, Steva and Mitrić, Miodrag and Pavlović, Vladimir B. and Dimitrijević-Branković, Suzana I.",
year = "2018",
abstract = "Two bimetallic (Zn/Cu) alginate based nanocomposites, impregnated with carbonate or phosphate mineral phase, were prepared by a facile procedure. Mineralized samples exhibited different morphologies and properties when compared to the non-mineralized sample. Antimicrobial testing against Escherichia coil, Staphylococcus aureus and Candida albicans showed that mineralized samples are more efficient than non-mineralized in elimination of microorganisms. The results of this study suggest that bimetallic mineralized alginates could be potentially used as affordable, easy to produce antimicrobial materials. (C) 2017 Elsevier By. All rights reserved.",
journal = "Materials Letters",
title = "Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application",
volume = "212",
pages = "32-36",
doi = "10.1016/j.matlet.2017.10.046"
}

Malagurski, I., Lević, S., Mitrić, M., Pavlović, V. B.,& Dimitrijević-Branković, S. I.. (2018). Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application. in Materials Letters, 212, 32-36.
https://doi.org/10.1016/j.matlet.2017.10.046

Malagurski I, Lević S, Mitrić M, Pavlović VB, Dimitrijević-Branković SI. Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application. in Materials Letters. 2018;212:32-36.
doi:10.1016/j.matlet.2017.10.046 .

Malagurski, Ivana, Lević, Steva, Mitrić, Miodrag, Pavlović, Vladimir B., Dimitrijević-Branković, Suzana I., "Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application" in Materials Letters, 212 (2018):32-36,
https://doi.org/10.1016/j.matlet.2017.10.046 . .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Milutinović, Milica
AU  - Mihajlovski, Katarina 
AU  - Jović, Jelena M.
AU  - Nastasijević, Branislav J.
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana I.
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0882401017312664
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7679
AB  - In the current study, the biocontrol potential of a novel strain Bacillus sp. PPM3 isolated from marine sediment from the Red Sea in Hurghada, Egypt is recognized. This novel strain was selected out of 32 isolates based on its ability to suppress the growth of four plant pathogenic fungi: Aspergillus flavus, Fusarium graminearum, Mucor sp. and Alternaria sp. The new marine strain was identified and characterized by phenotypic and molecular approaches. The culture filtrate of Bacillus sp. PPM3 suppressed the growth and spore germination of all tested fungi in vitro with the highest value of inhibition reported for Mucor sp. (97.5%). The antifungal effect of the culture filtrate from the strain PPM3 was due to production of highly stable secondary metabolites resistant to extreme pH, temperature and enzymatic treatments. A PCR analysis confirmed the expression of genes involved in the synthesis of antifungal lipopeptides: iturin, bacillomycin D, mycosubtilin and surfactin. In a greenhouse experiment strain PPM3 effectively reduced disease incidence of F. graminearum in maize plants and displayed additional plant growth stimulating effect. The results show that novel marine strain PPM3 could have a potential in commercial application as biocontrol agent for treatment of various plant diseases caused by soil-borne and postharvest pathogenic fungi.
T2  - Microbial Pathogenesis
T1  - Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment
VL  - 120
SP  - 71
EP  - 78
DO  - 10.1016/j.micpath.2018.04.056
ER  - 

@article{
author = "Radovanović, Neda and Milutinović, Milica and Mihajlovski, Katarina  and Jović, Jelena M. and Nastasijević, Branislav J. and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana I.",
year = "2018",
abstract = "In the current study, the biocontrol potential of a novel strain Bacillus sp. PPM3 isolated from marine sediment from the Red Sea in Hurghada, Egypt is recognized. This novel strain was selected out of 32 isolates based on its ability to suppress the growth of four plant pathogenic fungi: Aspergillus flavus, Fusarium graminearum, Mucor sp. and Alternaria sp. The new marine strain was identified and characterized by phenotypic and molecular approaches. The culture filtrate of Bacillus sp. PPM3 suppressed the growth and spore germination of all tested fungi in vitro with the highest value of inhibition reported for Mucor sp. (97.5%). The antifungal effect of the culture filtrate from the strain PPM3 was due to production of highly stable secondary metabolites resistant to extreme pH, temperature and enzymatic treatments. A PCR analysis confirmed the expression of genes involved in the synthesis of antifungal lipopeptides: iturin, bacillomycin D, mycosubtilin and surfactin. In a greenhouse experiment strain PPM3 effectively reduced disease incidence of F. graminearum in maize plants and displayed additional plant growth stimulating effect. The results show that novel marine strain PPM3 could have a potential in commercial application as biocontrol agent for treatment of various plant diseases caused by soil-borne and postharvest pathogenic fungi.",
journal = "Microbial Pathogenesis",
title = "Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment",
volume = "120",
pages = "71-78",
doi = "10.1016/j.micpath.2018.04.056"
}

Radovanović, N., Milutinović, M., Mihajlovski, K., Jović, J. M., Nastasijević, B. J., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S. I.. (2018). Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment. in Microbial Pathogenesis, 120, 71-78.
https://doi.org/10.1016/j.micpath.2018.04.056

Radovanović N, Milutinović M, Mihajlovski K, Jović JM, Nastasijević BJ, Rajilić-Stojanović M, Dimitrijević-Branković SI. Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment. in Microbial Pathogenesis. 2018;120:71-78.
doi:10.1016/j.micpath.2018.04.056 .

Radovanović, Neda, Milutinović, Milica, Mihajlovski, Katarina , Jović, Jelena M., Nastasijević, Branislav J., Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana I., "Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment" in Microbial Pathogenesis, 120 (2018):71-78,
https://doi.org/10.1016/j.micpath.2018.04.056 . .

TY  - CONF
AU  - Radovanović, Neda
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Malagurski, Ivana
AU  - Gordić, Milan V.
AU  - Nešić, Aleksandra
AU  - Dimitrijević-Branković, Suzana I.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10724
AB  - Polysaccharide-based materials represent an attractive alternative to plastics, due to their biodegradability, compatibility and great film forming properties1. As they are usually characterized by poor mechanical and barrier properties and lack of functionality, different components must be incorporated into these biopolymer materials in order to improve their properties. In this study, new mineralized, agar-based composite films with increasing Cu-phosphate mineral phase loadings (1, 2.5 and 5 mM) were prepared by in situ mineralization and solvent casting method. The presence of mineral significantly influenced the morphology, properties and fun- ctionality of the obtained composite films. Reinforcement with the Cu-phosphate phase improved in a concentration-dependent manner, optical, mechanical and water vapor barrier properties of the obtained mine- ralized films. In addition Cu-phosphate mineralized agar films exhibited antimicrobial activity against both, Gram positive and Gram negative bacteria, Staphylococcus aureus and Escherichia coli, respectively. The res- ults of this study suggest that agar films mineralized with Cu-phosphate could be potentially used as affordable, eco-friendly and functional food packaging materials with tunable properties. Production procedure offers possibilities for increasing Cu-mineral phase content without compromising properties of the composite films.
C3  - PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia
T1  - Active agar mineralized composite films intended for food packaging
SP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10724
ER  - 

@conference{
author = "Radovanović, Neda and Miljković, Miona and Davidović, Slađana and Malagurski, Ivana and Gordić, Milan V. and Nešić, Aleksandra and Dimitrijević-Branković, Suzana I.",
year = "2018",
abstract = "Polysaccharide-based materials represent an attractive alternative to plastics, due to their biodegradability, compatibility and great film forming properties1. As they are usually characterized by poor mechanical and barrier properties and lack of functionality, different components must be incorporated into these biopolymer materials in order to improve their properties. In this study, new mineralized, agar-based composite films with increasing Cu-phosphate mineral phase loadings (1, 2.5 and 5 mM) were prepared by in situ mineralization and solvent casting method. The presence of mineral significantly influenced the morphology, properties and fun- ctionality of the obtained composite films. Reinforcement with the Cu-phosphate phase improved in a concentration-dependent manner, optical, mechanical and water vapor barrier properties of the obtained mine- ralized films. In addition Cu-phosphate mineralized agar films exhibited antimicrobial activity against both, Gram positive and Gram negative bacteria, Staphylococcus aureus and Escherichia coli, respectively. The res- ults of this study suggest that agar films mineralized with Cu-phosphate could be potentially used as affordable, eco-friendly and functional food packaging materials with tunable properties. Production procedure offers possibilities for increasing Cu-mineral phase content without compromising properties of the composite films.",
journal = "PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia",
title = "Active agar mineralized composite films intended for food packaging",
pages = "94",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10724"
}

Radovanović, N., Miljković, M., Davidović, S., Malagurski, I., Gordić, M. V., Nešić, A.,& Dimitrijević-Branković, S. I.. (2018). Active agar mineralized composite films intended for food packaging. in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia, 94.
https://hdl.handle.net/21.15107/rcub_vinar_10724

Radovanović N, Miljković M, Davidović S, Malagurski I, Gordić MV, Nešić A, Dimitrijević-Branković SI. Active agar mineralized composite films intended for food packaging. in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia. 2018;:94.
https://hdl.handle.net/21.15107/rcub_vinar_10724 .

Radovanović, Neda, Miljković, Miona, Davidović, Slađana, Malagurski, Ivana, Gordić, Milan V., Nešić, Aleksandra, Dimitrijević-Branković, Suzana I., "Active agar mineralized composite films intended for food packaging" in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia (2018):94,
https://hdl.handle.net/21.15107/rcub_vinar_10724 .

TY  - JOUR
AU  - Bogdanović, Una
AU  - Dimitrijević, Suzana I.
AU  - Škapin, Srečo Davor
AU  - Popović, Maja
AU  - Rakočević, Zlatko Lj.
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Stoiljković, Milovan
AU  - Vodnik, Vesna
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0928493117326449
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7799
AB  - Copper nanoparticles (Cu NPs) have proven to own excellent antimicrobial efficacy, but the problems of easy oxidation and aggregation limit their practical application. Here, nanocomposite based on polyaniline (PANI) and Cu NPs solved this problem and brought additional physicochemical properties that are markedly advantageous for antimicrobial applications. Current work exploits this potential, to examine its time- and concentration-dependent antimicrobial activity, employing E. coli, S. aureus, and C. albicans as a model microbial species. Regarding the presence of polaronic charge carriers in the fibrous polyaniline network, effects of Cu NPs’ size and their partially oxidized surfaces (the data were confirmed by HRTEM, FESEM, XRD, Raman and XPS analysis), as well as rapid copper ions release, Cu-PANI nanocomposite showed efficient bactericidal and fungicidal activities at the concentrations ≤1 ppm, within the incubation time of 2 h. Beside the quantitative analysis, the high levels of cellular disruption for all tested microbes were evidenced by atomic force microscopy. Moreover, the minimum inhibitory and bactericidal concentrations of the Cu-PANI nanocomposite were lower than those reported for other nanocomposites. Using such low concentrations is recognized as a good way to avoid its toxicity toward the environment. For this purpose, Cu-PANI nanocomposite is tested for its genotoxicity and influence on the oxidative status of the human cells in vitro.
T2  - Materials Science and Engineering: C
T1  - Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation
VL  - 93
SP  - 49
EP  - 60
DO  - 10.1016/j.msec.2018.07.067
ER  - 

@article{
author = "Bogdanović, Una and Dimitrijević, Suzana I. and Škapin, Srečo Davor and Popović, Maja and Rakočević, Zlatko Lj. and Leskovac, Andreja and Petrović, Sandra and Stoiljković, Milovan and Vodnik, Vesna",
year = "2018",
abstract = "Copper nanoparticles (Cu NPs) have proven to own excellent antimicrobial efficacy, but the problems of easy oxidation and aggregation limit their practical application. Here, nanocomposite based on polyaniline (PANI) and Cu NPs solved this problem and brought additional physicochemical properties that are markedly advantageous for antimicrobial applications. Current work exploits this potential, to examine its time- and concentration-dependent antimicrobial activity, employing E. coli, S. aureus, and C. albicans as a model microbial species. Regarding the presence of polaronic charge carriers in the fibrous polyaniline network, effects of Cu NPs’ size and their partially oxidized surfaces (the data were confirmed by HRTEM, FESEM, XRD, Raman and XPS analysis), as well as rapid copper ions release, Cu-PANI nanocomposite showed efficient bactericidal and fungicidal activities at the concentrations ≤1 ppm, within the incubation time of 2 h. Beside the quantitative analysis, the high levels of cellular disruption for all tested microbes were evidenced by atomic force microscopy. Moreover, the minimum inhibitory and bactericidal concentrations of the Cu-PANI nanocomposite were lower than those reported for other nanocomposites. Using such low concentrations is recognized as a good way to avoid its toxicity toward the environment. For this purpose, Cu-PANI nanocomposite is tested for its genotoxicity and influence on the oxidative status of the human cells in vitro.",
journal = "Materials Science and Engineering: C",
title = "Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation",
volume = "93",
pages = "49-60",
doi = "10.1016/j.msec.2018.07.067"
}

Bogdanović, U., Dimitrijević, S. I., Škapin, S. D., Popović, M., Rakočević, Z. Lj., Leskovac, A., Petrović, S., Stoiljković, M.,& Vodnik, V.. (2018). Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation. in Materials Science and Engineering: C, 93, 49-60.
https://doi.org/10.1016/j.msec.2018.07.067

Bogdanović U, Dimitrijević SI, Škapin SD, Popović M, Rakočević ZL, Leskovac A, Petrović S, Stoiljković M, Vodnik V. Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation. in Materials Science and Engineering: C. 2018;93:49-60.
doi:10.1016/j.msec.2018.07.067 .

Bogdanović, Una, Dimitrijević, Suzana I., Škapin, Srečo Davor, Popović, Maja, Rakočević, Zlatko Lj., Leskovac, Andreja, Petrović, Sandra, Stoiljković, Milovan, Vodnik, Vesna, "Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation" in Materials Science and Engineering: C, 93 (2018):49-60,
https://doi.org/10.1016/j.msec.2018.07.067 . .

TY  - JOUR
AU  - Davidović, Slađana Z.
AU  - Miljković, Miona G.
AU  - Tomić, Miloš
AU  - Gordić, Milan V.
AU  - Nešić, Aleksandra
AU  - Dimitrijević, Suzana I.
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1932
AB  - The aim of this study was to develop dextran-based edible films plasticized by sorbitol. In order to optimise the film-forming formulation, response surface methodology was used. The influence of dextran and sorbitol concentration on the mechanical and water vapour barrier properties of obtained films was investigated. The results showed that both parameters exhibited significant effect on the water vapour permeability of a film. Both dextran and sorbitol concentration had significant influence on tensile strength and elongation at break, whereas only sorbitol concentration had significant effect on Youngs modulus. After optimisation by desirability approach, it was found that a film made of 3.40 wt% of dextran and 20.43 wt% of sorbitol showed the lowest water vapour permeability and the highest tensile strength and elasticity.
T2  - Carbohydrate Polymers
T1  - Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3
VL  - 184
SP  - 207
EP  - 213
DO  - 10.1016/j.carbpol.2017.12.061
ER  - 

@article{
author = "Davidović, Slađana Z. and Miljković, Miona G. and Tomić, Miloš and Gordić, Milan V. and Nešić, Aleksandra and Dimitrijević, Suzana I.",
year = "2018",
abstract = "The aim of this study was to develop dextran-based edible films plasticized by sorbitol. In order to optimise the film-forming formulation, response surface methodology was used. The influence of dextran and sorbitol concentration on the mechanical and water vapour barrier properties of obtained films was investigated. The results showed that both parameters exhibited significant effect on the water vapour permeability of a film. Both dextran and sorbitol concentration had significant influence on tensile strength and elongation at break, whereas only sorbitol concentration had significant effect on Youngs modulus. After optimisation by desirability approach, it was found that a film made of 3.40 wt% of dextran and 20.43 wt% of sorbitol showed the lowest water vapour permeability and the highest tensile strength and elasticity.",
journal = "Carbohydrate Polymers",
title = "Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3",
volume = "184",
pages = "207-213",
doi = "10.1016/j.carbpol.2017.12.061"
}

Davidović, S. Z., Miljković, M. G., Tomić, M., Gordić, M. V., Nešić, A.,& Dimitrijević, S. I.. (2018). Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3. in Carbohydrate Polymers, 184, 207-213.
https://doi.org/10.1016/j.carbpol.2017.12.061

Davidović SZ, Miljković MG, Tomić M, Gordić MV, Nešić A, Dimitrijević SI. Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3. in Carbohydrate Polymers. 2018;184:207-213.
doi:10.1016/j.carbpol.2017.12.061 .

Davidović, Slađana Z., Miljković, Miona G., Tomić, Miloš, Gordić, Milan V., Nešić, Aleksandra, Dimitrijević, Suzana I., "Response surface methodology for optimisation of edible coatings based on dextran from Leuconostoc mesenteroides T3" in Carbohydrate Polymers, 184 (2018):207-213,
https://doi.org/10.1016/j.carbpol.2017.12.061 . .

TY  - CONF
AU  - Davidović, Slađana
AU  - Miljković, Miona
AU  - Radovanović, Neda
AU  - Gordić, Milan V.
AU  - Nešić, Aleksandra
AU  - Dimitrijević, Suzana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10722
AB  - Current trends in food biotechnology are focused on replacing synthetic polymers used for food packages by the natural ones. Biopolymers are made by sustainable processes in a cheap way. Moreover, they are nontoxic and can serve as a good alternative to traditional (petroleum) food packaging, due to great film-formation properties in a form of edible films and coatings for food products. Various polysaccharides, proteins, and lipids have been intensively investigated as edible materials that could improve the food quality, freshness, and provide food safety. However, natural polymers usually lack of antioxidative and/or antimicrobial properties. Therefore, many synthetic and natural additives can be incorporated into edible films. Among them, extracts of medicinal plants are giving more attention due to their safeness and proved health benefits. The aim of this study was to obtain edible films based on dextran, with antioxidative and antimicrobial pro- perties. Dextran obtained in our lab was used for preparing edible film with sorbitol as a plasticizer, while pulverized waste biomass obtained from the processing of medicinal herb yarrow (Achillea millefolium) was used as a source of polyphenols. It has previously been shown 1 that dextran-based films had excellent mecha- nical and water vapor barrier properties. In this work, spray dried ethanol extract of Achillea millefolium dust (AME) was incorporated into dextran films in concentrations 2, 3, and 4% (w/v). The composite films were analyzed regarding antioxidative, antimicrobial, and mechanical properties. All films showed high antioxidative activity (90% reduction of DPPH radical). However, to obtain high antimicrobial activity (higher than 50%), 4% of AME was required. Composite films showed antimicrobial activity against three Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and Enterococcus faecalis and one Gram-negative bacterium (Pseudomonas aeruginosa). Incorporation of AME into dextran film showed positive effect on tensile strength and negative effect on elongation. Composite film with the best mechanical properties (tensile strength 3.5 MPa and elongation at break 37%) was the film containing 4% AME.
C3  - PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia
T1  - Antioxidant and antimicrobial edible films based on dextran containing polyphenols from yarrow extract
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10722
ER  - 

@conference{
author = "Davidović, Slađana and Miljković, Miona and Radovanović, Neda and Gordić, Milan V. and Nešić, Aleksandra and Dimitrijević, Suzana",
year = "2018",
abstract = "Current trends in food biotechnology are focused on replacing synthetic polymers used for food packages by the natural ones. Biopolymers are made by sustainable processes in a cheap way. Moreover, they are nontoxic and can serve as a good alternative to traditional (petroleum) food packaging, due to great film-formation properties in a form of edible films and coatings for food products. Various polysaccharides, proteins, and lipids have been intensively investigated as edible materials that could improve the food quality, freshness, and provide food safety. However, natural polymers usually lack of antioxidative and/or antimicrobial properties. Therefore, many synthetic and natural additives can be incorporated into edible films. Among them, extracts of medicinal plants are giving more attention due to their safeness and proved health benefits. The aim of this study was to obtain edible films based on dextran, with antioxidative and antimicrobial pro- perties. Dextran obtained in our lab was used for preparing edible film with sorbitol as a plasticizer, while pulverized waste biomass obtained from the processing of medicinal herb yarrow (Achillea millefolium) was used as a source of polyphenols. It has previously been shown 1 that dextran-based films had excellent mecha- nical and water vapor barrier properties. In this work, spray dried ethanol extract of Achillea millefolium dust (AME) was incorporated into dextran films in concentrations 2, 3, and 4% (w/v). The composite films were analyzed regarding antioxidative, antimicrobial, and mechanical properties. All films showed high antioxidative activity (90% reduction of DPPH radical). However, to obtain high antimicrobial activity (higher than 50%), 4% of AME was required. Composite films showed antimicrobial activity against three Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and Enterococcus faecalis and one Gram-negative bacterium (Pseudomonas aeruginosa). Incorporation of AME into dextran film showed positive effect on tensile strength and negative effect on elongation. Composite film with the best mechanical properties (tensile strength 3.5 MPa and elongation at break 37%) was the film containing 4% AME.",
journal = "PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia",
title = "Antioxidant and antimicrobial edible films based on dextran containing polyphenols from yarrow extract",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10722"
}

Davidović, S., Miljković, M., Radovanović, N., Gordić, M. V., Nešić, A.,& Dimitrijević, S.. (2018). Antioxidant and antimicrobial edible films based on dextran containing polyphenols from yarrow extract. in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia, 72.
https://hdl.handle.net/21.15107/rcub_vinar_10722

Davidović S, Miljković M, Radovanović N, Gordić MV, Nešić A, Dimitrijević S. Antioxidant and antimicrobial edible films based on dextran containing polyphenols from yarrow extract. in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia. 2018;:72.
https://hdl.handle.net/21.15107/rcub_vinar_10722 .

Davidović, Slađana, Miljković, Miona, Radovanović, Neda, Gordić, Milan V., Nešić, Aleksandra, Dimitrijević, Suzana, "Antioxidant and antimicrobial edible films based on dextran containing polyphenols from yarrow extract" in PolyChar 26th annual world forum on advanced materials, September 10-13, 2018, Tbilisi, Georgia (2018):72,
https://hdl.handle.net/21.15107/rcub_vinar_10722 .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Davidović, Slađana Z.
AU  - Veljović, Đorđe N.
AU  - Carević, Milica B.
AU  - Lazić, Vesna M.
AU  - Dimitrijević-Branković, Suzana I.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1866
AB  - The agricultural raw industry generates large amounts of annually by-products that create disposal problems. Hitherto, there have been no reported papers about the simultaneous production of cellulase and beta-amylase from these raw materials using Paenibacillus sp. that would reduce the costs. Thus, in this paper simultaneous cellulase (CMC-ase and avicelase) and beta-amylase production using barley bran and the application of the natural isolate Paenibacillus chitinolyticus CKS1 and potential enzymes in the hydrolysis process was studied. Response surface methodology was used to obtain the maximum enzyme activity (CMC-ase 0.405 U mL(-1), avicelase 0.433 U mL(-1) and beta-amylase 1.594U mL(-1)). Scanning electron microscopy showed degradation of the lignocellulosic-starch structure of barley bran after fermentation. The CKS1 bacterial supernatant, which contains cellulases and beta-amylase, could hydrolyze cotton fibres and barley bran, respectively. The main products after enzymatic hydrolysis of cotton fibres and barley bran, glucose (0.117 (-1)(g gmat)) and maltose (0.347 (-1)(g gmat)), were quantified by high performance liquid chromatography (HPLC). The produced enzymes could be used for hydrolysis of cotton fabric and barley bran to glucose and maltose, respectively. Application of simultaneous enzymes production using an agricultural by-product is economically and environmentally accepted and moreover, valuable biotechnological products, such as glucose and maltose, were obtained in this investigation.
T2  - Journal of the Serbian Chemical Society
T1  - Effective valorisation of barley bran for simultaneous cellulase and beta-amylase production by Paenibacillus chitinolyticus CKS1: Statistical optimization and enzymes application
VL  - 82
IS  - 11
SP  - 1223
EP  - 1236
DO  - 10.2298/JSC170514092M
ER  - 

@article{
author = "Mihajlovski, Katarina and Davidović, Slađana Z. and Veljović, Đorđe N. and Carević, Milica B. and Lazić, Vesna M. and Dimitrijević-Branković, Suzana I.",
year = "2017",
abstract = "The agricultural raw industry generates large amounts of annually by-products that create disposal problems. Hitherto, there have been no reported papers about the simultaneous production of cellulase and beta-amylase from these raw materials using Paenibacillus sp. that would reduce the costs. Thus, in this paper simultaneous cellulase (CMC-ase and avicelase) and beta-amylase production using barley bran and the application of the natural isolate Paenibacillus chitinolyticus CKS1 and potential enzymes in the hydrolysis process was studied. Response surface methodology was used to obtain the maximum enzyme activity (CMC-ase 0.405 U mL(-1), avicelase 0.433 U mL(-1) and beta-amylase 1.594U mL(-1)). Scanning electron microscopy showed degradation of the lignocellulosic-starch structure of barley bran after fermentation. The CKS1 bacterial supernatant, which contains cellulases and beta-amylase, could hydrolyze cotton fibres and barley bran, respectively. The main products after enzymatic hydrolysis of cotton fibres and barley bran, glucose (0.117 (-1)(g gmat)) and maltose (0.347 (-1)(g gmat)), were quantified by high performance liquid chromatography (HPLC). The produced enzymes could be used for hydrolysis of cotton fabric and barley bran to glucose and maltose, respectively. Application of simultaneous enzymes production using an agricultural by-product is economically and environmentally accepted and moreover, valuable biotechnological products, such as glucose and maltose, were obtained in this investigation.",
journal = "Journal of the Serbian Chemical Society",
title = "Effective valorisation of barley bran for simultaneous cellulase and beta-amylase production by Paenibacillus chitinolyticus CKS1: Statistical optimization and enzymes application",
volume = "82",
number = "11",
pages = "1223-1236",
doi = "10.2298/JSC170514092M"
}

Mihajlovski, K., Davidović, S. Z., Veljović, Đ. N., Carević, M. B., Lazić, V. M.,& Dimitrijević-Branković, S. I.. (2017). Effective valorisation of barley bran for simultaneous cellulase and beta-amylase production by Paenibacillus chitinolyticus CKS1: Statistical optimization and enzymes application. in Journal of the Serbian Chemical Society, 82(11), 1223-1236.
https://doi.org/10.2298/JSC170514092M

Mihajlovski K, Davidović SZ, Veljović ĐN, Carević MB, Lazić VM, Dimitrijević-Branković SI. Effective valorisation of barley bran for simultaneous cellulase and beta-amylase production by Paenibacillus chitinolyticus CKS1: Statistical optimization and enzymes application. in Journal of the Serbian Chemical Society. 2017;82(11):1223-1236.
doi:10.2298/JSC170514092M .

Mihajlovski, Katarina, Davidović, Slađana Z., Veljović, Đorđe N., Carević, Milica B., Lazić, Vesna M., Dimitrijević-Branković, Suzana I., "Effective valorisation of barley bran for simultaneous cellulase and beta-amylase production by Paenibacillus chitinolyticus CKS1: Statistical optimization and enzymes application" in Journal of the Serbian Chemical Society, 82, no. 11 (2017):1223-1236,
https://doi.org/10.2298/JSC170514092M . .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Gordić, Milan V.
AU  - Onjia, Antonije E.
AU  - Davidović, Slađana Z.
AU  - Miljković, Miona G.
AU  - Dimitrijević-Branković, Suzana I.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1344
AB  - In this work, antimicrobial bioinspired films made from chitosan incorporated with triclosan were investigated. The tensile strenght of these films were in the range of 33 and 39 MPa, which presented satisfied mechanical stability comparable to the synthetic-based packages commonly used in industry. The addition of triclosan enhanced thermal stability and antimicrobial activity of chitosan films against Escherichia coli and Staphylococus aureus. Results obtained in this work demonstrated that chitosan/triclosan films could be potentially used as an eco-sustainable package in healthcare sector to prevent infections/contaminations.
T2  - Materials Letters
T1  - Chitosan-triclosan films for potential use as bio-antimicrobial bags in healthcare sector
VL  - 186
SP  - 368
EP  - 371
DO  - 10.1016/j.matlet.2016.10.028
ER  - 

@article{
author = "Nešić, Aleksandra and Gordić, Milan V. and Onjia, Antonije E. and Davidović, Slađana Z. and Miljković, Miona G. and Dimitrijević-Branković, Suzana I.",
year = "2017",
abstract = "In this work, antimicrobial bioinspired films made from chitosan incorporated with triclosan were investigated. The tensile strenght of these films were in the range of 33 and 39 MPa, which presented satisfied mechanical stability comparable to the synthetic-based packages commonly used in industry. The addition of triclosan enhanced thermal stability and antimicrobial activity of chitosan films against Escherichia coli and Staphylococus aureus. Results obtained in this work demonstrated that chitosan/triclosan films could be potentially used as an eco-sustainable package in healthcare sector to prevent infections/contaminations.",
journal = "Materials Letters",
title = "Chitosan-triclosan films for potential use as bio-antimicrobial bags in healthcare sector",
volume = "186",
pages = "368-371",
doi = "10.1016/j.matlet.2016.10.028"
}

Nešić, A., Gordić, M. V., Onjia, A. E., Davidović, S. Z., Miljković, M. G.,& Dimitrijević-Branković, S. I.. (2017). Chitosan-triclosan films for potential use as bio-antimicrobial bags in healthcare sector. in Materials Letters, 186, 368-371.
https://doi.org/10.1016/j.matlet.2016.10.028

Nešić A, Gordić MV, Onjia AE, Davidović SZ, Miljković MG, Dimitrijević-Branković SI. Chitosan-triclosan films for potential use as bio-antimicrobial bags in healthcare sector. in Materials Letters. 2017;186:368-371.
doi:10.1016/j.matlet.2016.10.028 .

Nešić, Aleksandra, Gordić, Milan V., Onjia, Antonije E., Davidović, Slađana Z., Miljković, Miona G., Dimitrijević-Branković, Suzana I., "Chitosan-triclosan films for potential use as bio-antimicrobial bags in healthcare sector" in Materials Letters, 186 (2017):368-371,
https://doi.org/10.1016/j.matlet.2016.10.028 . .

TY  - JOUR
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Nešić, Aleksandra
AU  - Mitrić, Miodrag
AU  - Pavlović, Vladimir B.
AU  - Dimitrijević-Branković, Suzana I.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1731
AB  - New mineralized, agar-based nanocomposite films (Zn-carbonate and Zn-phosphate/agar) were produced by a combination of in situ precipitation and a casting method. The presence of minerals significantly influenced the morphology, properties and functionality of the obtained nanocomposites. Reinforcement with the Zn-mineral phase improved the mechanical properties of the carbonate-mineralized films, but had a negligible effect on the phosphate-mineralized samples. Both nanocomposites showed improved optical and thermal properties, better Zn(II) release potential in a slightly acidic environment and exhibited antimicrobial activity against S. aureus. These results suggest that Zn-mineralized agar nanocomposite films could be potentially used as affordable, eco-friendly and active food packaging materials. (C) 2017 Elsevier Ltd. All rights reserved.
T2  - Carbohydrate Polymers
T1  - Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties
VL  - 175
SP  - 55
EP  - 62
DO  - 10.1016/j.carbpol.2017.07.064
ER  - 

@article{
author = "Malagurski, Ivana and Lević, Steva and Nešić, Aleksandra and Mitrić, Miodrag and Pavlović, Vladimir B. and Dimitrijević-Branković, Suzana I.",
year = "2017",
abstract = "New mineralized, agar-based nanocomposite films (Zn-carbonate and Zn-phosphate/agar) were produced by a combination of in situ precipitation and a casting method. The presence of minerals significantly influenced the morphology, properties and functionality of the obtained nanocomposites. Reinforcement with the Zn-mineral phase improved the mechanical properties of the carbonate-mineralized films, but had a negligible effect on the phosphate-mineralized samples. Both nanocomposites showed improved optical and thermal properties, better Zn(II) release potential in a slightly acidic environment and exhibited antimicrobial activity against S. aureus. These results suggest that Zn-mineralized agar nanocomposite films could be potentially used as affordable, eco-friendly and active food packaging materials. (C) 2017 Elsevier Ltd. All rights reserved.",
journal = "Carbohydrate Polymers",
title = "Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties",
volume = "175",
pages = "55-62",
doi = "10.1016/j.carbpol.2017.07.064"
}

Malagurski, I., Lević, S., Nešić, A., Mitrić, M., Pavlović, V. B.,& Dimitrijević-Branković, S. I.. (2017). Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties. in Carbohydrate Polymers, 175, 55-62.
https://doi.org/10.1016/j.carbpol.2017.07.064

Malagurski I, Lević S, Nešić A, Mitrić M, Pavlović VB, Dimitrijević-Branković SI. Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties. in Carbohydrate Polymers. 2017;175:55-62.
doi:10.1016/j.carbpol.2017.07.064 .

Malagurski, Ivana, Lević, Steva, Nešić, Aleksandra, Mitrić, Miodrag, Pavlović, Vladimir B., Dimitrijević-Branković, Suzana I., "Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties" in Carbohydrate Polymers, 175 (2017):55-62,
https://doi.org/10.1016/j.carbpol.2017.07.064 . .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Ružić, Jovana
AU  - Gordić, Milan V.
AU  - Ostojić, Sanja 
AU  - Micić, Darko M.
AU  - Onjia, Antonije E.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1446
AB  - In this work, the new biodegradable blend films composed of natural polymer pectin and synthetic polymer polyvinylpyrrolidone were obtained through casting process. The concentration of glycerol in all formulations was constant (2% v/v), while the amount of polyvinylpyrrolidone varied from 20 to 60% by mass of pectin. The molecular interactions between two components were elucidated by FTIR-ATR spectroscopy, while functional properties of these films were carried out by determination of puncture resistance, tensile strength, thermal stability, water vapor barrier, wettability and printability. The blending of pectin with PVP led to an improvement of mechanical resistance, barrier properties and hydrophobicity of these films for 57%, 58 and 24%, respectively, remaining adequate thermal stability. Since pectin and PVP are nontoxic, food-grade and biodegradable materials, this research present an ecosustainable approach to provide films with satisfied physical properties for use as packaging material of non-food products, such as water-treatment products (detergents, biocides, agrochemical compounds). (C) 2016 Elsevier Ltd. All rights reserved.
T2  - Composites: Part B: Engineering
T1  - Pectin-polyvinylpyrrolidone films: A sustainable approach to the development of biobased packaging materials
VL  - 110
SP  - 56
EP  - 61
DO  - 10.1016/j.compositesb.2016.11.016
ER  - 

@article{
author = "Nešić, Aleksandra and Ružić, Jovana and Gordić, Milan V. and Ostojić, Sanja  and Micić, Darko M. and Onjia, Antonije E.",
year = "2017",
abstract = "In this work, the new biodegradable blend films composed of natural polymer pectin and synthetic polymer polyvinylpyrrolidone were obtained through casting process. The concentration of glycerol in all formulations was constant (2% v/v), while the amount of polyvinylpyrrolidone varied from 20 to 60% by mass of pectin. The molecular interactions between two components were elucidated by FTIR-ATR spectroscopy, while functional properties of these films were carried out by determination of puncture resistance, tensile strength, thermal stability, water vapor barrier, wettability and printability. The blending of pectin with PVP led to an improvement of mechanical resistance, barrier properties and hydrophobicity of these films for 57%, 58 and 24%, respectively, remaining adequate thermal stability. Since pectin and PVP are nontoxic, food-grade and biodegradable materials, this research present an ecosustainable approach to provide films with satisfied physical properties for use as packaging material of non-food products, such as water-treatment products (detergents, biocides, agrochemical compounds). (C) 2016 Elsevier Ltd. All rights reserved.",
journal = "Composites: Part B: Engineering",
title = "Pectin-polyvinylpyrrolidone films: A sustainable approach to the development of biobased packaging materials",
volume = "110",
pages = "56-61",
doi = "10.1016/j.compositesb.2016.11.016"
}

Nešić, A., Ružić, J., Gordić, M. V., Ostojić, S., Micić, D. M.,& Onjia, A. E.. (2017). Pectin-polyvinylpyrrolidone films: A sustainable approach to the development of biobased packaging materials. in Composites: Part B: Engineering, 110, 56-61.
https://doi.org/10.1016/j.compositesb.2016.11.016

Nešić A, Ružić J, Gordić MV, Ostojić S, Micić DM, Onjia AE. Pectin-polyvinylpyrrolidone films: A sustainable approach to the development of biobased packaging materials. in Composites: Part B: Engineering. 2017;110:56-61.
doi:10.1016/j.compositesb.2016.11.016 .

Nešić, Aleksandra, Ružić, Jovana, Gordić, Milan V., Ostojić, Sanja , Micić, Darko M., Onjia, Antonije E., "Pectin-polyvinylpyrrolidone films: A sustainable approach to the development of biobased packaging materials" in Composites: Part B: Engineering, 110 (2017):56-61,
https://doi.org/10.1016/j.compositesb.2016.11.016 . .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Onjia, Antonije E.
AU  - Davidović, Slađana Z.
AU  - Dimitrijević, Suzana I.
AU  - Errico, Maria Emanuela
AU  - Santagata, Gabriella
AU  - Malinconico, Mario
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1385
AB  - In this study, pectin based films including different amounts of sodium alginate were prepared by casting method. All the films, with and without polyglycerol as plasticizer, were crosslinked with zinc ions in order to extend their potential functionality. The development of junction points, occurring during the crosslinking process with zinc ions, induced the increasing of free volume with following changing in chemico-physical properties of films. The inclusion of alginate in pectin based formulations improved the strength of zinc ions crosslinking network, whereas the addition of polyglycerol significantly improved mechanical performance. Finally, zinc-crosslinked films evidenced antimicrobial activity against the most common exploited pathogens: Staphylococcus Aureus, Escherichia Coli and Candida Albicans. These results suggest that zinc-crosslinked based films can be exploitable as novel bio-active biomaterials for protection and disinfection of medical devices. (C) 2016 Elsevier Ltd. All rights reserved.
T2  - Carbohydrate Polymers
T1  - Design of pectin-sodium alginate based films for potential healthcare application: Study of chemico-physical interactions between the components of films and assessment of their antimicrobial activity
VL  - 157
SP  - 981
EP  - 990
DO  - 10.1016/j.carbpol.2016.10.054
ER  - 

@article{
author = "Nešić, Aleksandra and Onjia, Antonije E. and Davidović, Slađana Z. and Dimitrijević, Suzana I. and Errico, Maria Emanuela and Santagata, Gabriella and Malinconico, Mario",
year = "2017",
abstract = "In this study, pectin based films including different amounts of sodium alginate were prepared by casting method. All the films, with and without polyglycerol as plasticizer, were crosslinked with zinc ions in order to extend their potential functionality. The development of junction points, occurring during the crosslinking process with zinc ions, induced the increasing of free volume with following changing in chemico-physical properties of films. The inclusion of alginate in pectin based formulations improved the strength of zinc ions crosslinking network, whereas the addition of polyglycerol significantly improved mechanical performance. Finally, zinc-crosslinked films evidenced antimicrobial activity against the most common exploited pathogens: Staphylococcus Aureus, Escherichia Coli and Candida Albicans. These results suggest that zinc-crosslinked based films can be exploitable as novel bio-active biomaterials for protection and disinfection of medical devices. (C) 2016 Elsevier Ltd. All rights reserved.",
journal = "Carbohydrate Polymers",
title = "Design of pectin-sodium alginate based films for potential healthcare application: Study of chemico-physical interactions between the components of films and assessment of their antimicrobial activity",
volume = "157",
pages = "981-990",
doi = "10.1016/j.carbpol.2016.10.054"
}

Nešić, A., Onjia, A. E., Davidović, S. Z., Dimitrijević, S. I., Errico, M. E., Santagata, G.,& Malinconico, M.. (2017). Design of pectin-sodium alginate based films for potential healthcare application: Study of chemico-physical interactions between the components of films and assessment of their antimicrobial activity. in Carbohydrate Polymers, 157, 981-990.
https://doi.org/10.1016/j.carbpol.2016.10.054

Nešić A, Onjia AE, Davidović SZ, Dimitrijević SI, Errico ME, Santagata G, Malinconico M. Design of pectin-sodium alginate based films for potential healthcare application: Study of chemico-physical interactions between the components of films and assessment of their antimicrobial activity. in Carbohydrate Polymers. 2017;157:981-990.
doi:10.1016/j.carbpol.2016.10.054 .

Nešić, Aleksandra, Onjia, Antonije E., Davidović, Slađana Z., Dimitrijević, Suzana I., Errico, Maria Emanuela, Santagata, Gabriella, Malinconico, Mario, "Design of pectin-sodium alginate based films for potential healthcare application: Study of chemico-physical interactions between the components of films and assessment of their antimicrobial activity" in Carbohydrate Polymers, 157 (2017):981-990,
https://doi.org/10.1016/j.carbpol.2016.10.054 . .

TY  - JOUR
AU  - Davidović, Slađana Z.
AU  - Lazić, Vesna M.
AU  - Vukoje, Ivana D.
AU  - Papan, Jelena
AU  - Anhrenkiel, S. Phillip
AU  - Dimitrijević, Suzana I.
AU  - Nedeljković, Jovan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1887
AB  - A simple, fast and non-costly method for selective cysteine (Cys) detection, based on optical changes of silver colloids, is developed. For that purpose, stable colloids consisting of silver nanoparticles (Ag NPs) coated with polysaccharide dextran (Dex), isolated from bacterium species Leuconostoc mesenteroides T3, were prepared. The synthesized samples were thoroughly characterized including absorption and FTIR spectroscopy, as well as transmission electron microscopy and X-ray diffraction analysis. The silver colloids display high sensitivity and selectivity towards Cys detection in aqueous solutions. The Ag NPs coated with Dex provide possibility to detect Cys among a dozen amino acids and its detection limit was found to be 12.0 M. The sensing mechanism - red shift of optical absorption - is discussed in terms of the agglomeration of Ag NPs due to formation of hydrogen bonds between Cys molecules attached to different Ag NPs. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Dextran coated silver nanoparticles Chemical sensor for selective cysteine detection
VL  - 160
SP  - 184
EP  - 191
DO  - 10.1016/j.colsurfb.2017.09.031
ER  - 

@article{
author = "Davidović, Slađana Z. and Lazić, Vesna M. and Vukoje, Ivana D. and Papan, Jelena and Anhrenkiel, S. Phillip and Dimitrijević, Suzana I. and Nedeljković, Jovan",
year = "2017",
abstract = "A simple, fast and non-costly method for selective cysteine (Cys) detection, based on optical changes of silver colloids, is developed. For that purpose, stable colloids consisting of silver nanoparticles (Ag NPs) coated with polysaccharide dextran (Dex), isolated from bacterium species Leuconostoc mesenteroides T3, were prepared. The synthesized samples were thoroughly characterized including absorption and FTIR spectroscopy, as well as transmission electron microscopy and X-ray diffraction analysis. The silver colloids display high sensitivity and selectivity towards Cys detection in aqueous solutions. The Ag NPs coated with Dex provide possibility to detect Cys among a dozen amino acids and its detection limit was found to be 12.0 M. The sensing mechanism - red shift of optical absorption - is discussed in terms of the agglomeration of Ag NPs due to formation of hydrogen bonds between Cys molecules attached to different Ag NPs. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Dextran coated silver nanoparticles Chemical sensor for selective cysteine detection",
volume = "160",
pages = "184-191",
doi = "10.1016/j.colsurfb.2017.09.031"
}

Davidović, S. Z., Lazić, V. M., Vukoje, I. D., Papan, J., Anhrenkiel, S. P., Dimitrijević, S. I.,& Nedeljković, J.. (2017). Dextran coated silver nanoparticles Chemical sensor for selective cysteine detection. in Colloids and Surfaces. B: Biointerfaces, 160, 184-191.
https://doi.org/10.1016/j.colsurfb.2017.09.031

Davidović SZ, Lazić VM, Vukoje ID, Papan J, Anhrenkiel SP, Dimitrijević SI, Nedeljković J. Dextran coated silver nanoparticles Chemical sensor for selective cysteine detection. in Colloids and Surfaces. B: Biointerfaces. 2017;160:184-191.
doi:10.1016/j.colsurfb.2017.09.031 .

Davidović, Slađana Z., Lazić, Vesna M., Vukoje, Ivana D., Papan, Jelena, Anhrenkiel, S. Phillip, Dimitrijević, Suzana I., Nedeljković, Jovan, "Dextran coated silver nanoparticles Chemical sensor for selective cysteine detection" in Colloids and Surfaces. B: Biointerfaces, 160 (2017):184-191,
https://doi.org/10.1016/j.colsurfb.2017.09.031 . .

TY  - CONF
AU  - Bogdanović, Una
AU  - Vodnik, Vesna
AU  - Mitrić, Miodrag
AU  - Dimitrijević, Suzana I.
AU  - Škapin, Srečo Davor
AU  - Žunič, Vojka
AU  - Budimir, Milica
AU  - Stoiljković, Milovan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9257
AB  - By combining copper nanoparticles (CuNPs) as a good antimicrobial agent
with polyaniline (PANI), which also shows some degree of antimicrobial
activity, we were able to synthesize a novel promising antimicrobial
material – copper-polyaniline (Cu-PANI) nanocomposite. It was prepared
by simple in situ polymerization method, when thepolymer and metal
nanoparticles (dav= 6 nm)are produced simultaneously.Quantitative
(antimicrobial assay) and qualitative (atomic force microscopy – AFM)
analyses showed that synergestic effect of CuNPs and PANI against
bacteriaE. coli andS. aureus,and fungusC. albicans, provides its faster
andhigher antimicrobial activity than any component acting alone.This
makes it a great candidate for fast waste water treatment.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
T1  - Antimicrobial activity of copper-polyaniline nanocomposite
SP  - 653
EP  - 656
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9257
ER  - 

@conference{
author = "Bogdanović, Una and Vodnik, Vesna and Mitrić, Miodrag and Dimitrijević, Suzana I. and Škapin, Srečo Davor and Žunič, Vojka and Budimir, Milica and Stoiljković, Milovan",
year = "2016",
abstract = "By combining copper nanoparticles (CuNPs) as a good antimicrobial agent
with polyaniline (PANI), which also shows some degree of antimicrobial
activity, we were able to synthesize a novel promising antimicrobial
material – copper-polyaniline (Cu-PANI) nanocomposite. It was prepared
by simple in situ polymerization method, when thepolymer and metal
nanoparticles (dav= 6 nm)are produced simultaneously.Quantitative
(antimicrobial assay) and qualitative (atomic force microscopy – AFM)
analyses showed that synergestic effect of CuNPs and PANI against
bacteriaE. coli andS. aureus,and fungusC. albicans, provides its faster
andhigher antimicrobial activity than any component acting alone.This
makes it a great candidate for fast waste water treatment.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry",
title = "Antimicrobial activity of copper-polyaniline nanocomposite",
pages = "653-656",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9257"
}

Bogdanović, U., Vodnik, V., Mitrić, M., Dimitrijević, S. I., Škapin, S. D., Žunič, V., Budimir, M.,& Stoiljković, M.. (2016). Antimicrobial activity of copper-polyaniline nanocomposite. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 653-656.
https://hdl.handle.net/21.15107/rcub_vinar_9257

Bogdanović U, Vodnik V, Mitrić M, Dimitrijević SI, Škapin SD, Žunič V, Budimir M, Stoiljković M. Antimicrobial activity of copper-polyaniline nanocomposite. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry. 2016;:653-656.
https://hdl.handle.net/21.15107/rcub_vinar_9257 .

Bogdanović, Una, Vodnik, Vesna, Mitrić, Miodrag, Dimitrijević, Suzana I., Škapin, Srečo Davor, Žunič, Vojka, Budimir, Milica, Stoiljković, Milovan, "Antimicrobial activity of copper-polyaniline nanocomposite" in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry (2016):653-656,
https://hdl.handle.net/21.15107/rcub_vinar_9257 .

TY  - JOUR
AU  - Bogdanović, Una
AU  - Vodnik, Vesna
AU  - Mitrić, Miodrag
AU  - Dimitrijević, Suzana I.
AU  - Škapin, Srečo Davor
AU  - Zunic, Vojka
AU  - Budimir, Milica
AU  - Stoiljković, Milovan
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/376
AB  - This study explores different mechanisms of antimicrobial action by designing hybrid nanomaterials that provide a new approach in the fight against resistant microbes. Here, we present a cheap copper-polyaniline (Cu-PANI) nanocomposite material with enhanced antimicrobial properties, prepared by simple in situ polymerization method, when polymer and metal nanoparticles are produced simultaneously. The copper nanoparticles (CuNPs) are uniformly dispersed in the polymer and have a narrow size distribution (dav = 6 nm). We found that CuNPs and PANI act synergistically against three strains, Escherichia coli, Staphylococcus aureus, and Candida albicans, and resulting nanocomposite exhibits higher antimicrobial activity than any component acting alone. Before using the colony counting method to quantify its time and concentration antimicrobial activity, different techniques (UV-visible spectroscopy, transmission electron microscopy, scanning electron microscope, field emission scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectrophotometry, and inductively coupled plasma optical emission spectrometry) were used to identify the optical, structural, and chemical aspects of the formed Cu-PANI nanocomposite. The antimicrobial activity of this nanocomposite shows that the microbial growth has been fully inhibited; moreover, some of the tested microbes were killed. Atomic force microscopy revealed dramatic changes in morphology of tested cells due to disruption of their cell wall integrity after incubation with Cu-PANI nanocomposite.
T2  - ACS Applied Materials and Interfaces
T1  - Nanomaterial with High Antimicrobial Efficacy-Copper/Polyaniline Nanocomposite
VL  - 7
IS  - 3
SP  - 1955
EP  - 1966
DO  - 10.1021/am507746m
ER  - 

@article{
author = "Bogdanović, Una and Vodnik, Vesna and Mitrić, Miodrag and Dimitrijević, Suzana I. and Škapin, Srečo Davor and Zunic, Vojka and Budimir, Milica and Stoiljković, Milovan",
year = "2015",
abstract = "This study explores different mechanisms of antimicrobial action by designing hybrid nanomaterials that provide a new approach in the fight against resistant microbes. Here, we present a cheap copper-polyaniline (Cu-PANI) nanocomposite material with enhanced antimicrobial properties, prepared by simple in situ polymerization method, when polymer and metal nanoparticles are produced simultaneously. The copper nanoparticles (CuNPs) are uniformly dispersed in the polymer and have a narrow size distribution (dav = 6 nm). We found that CuNPs and PANI act synergistically against three strains, Escherichia coli, Staphylococcus aureus, and Candida albicans, and resulting nanocomposite exhibits higher antimicrobial activity than any component acting alone. Before using the colony counting method to quantify its time and concentration antimicrobial activity, different techniques (UV-visible spectroscopy, transmission electron microscopy, scanning electron microscope, field emission scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectrophotometry, and inductively coupled plasma optical emission spectrometry) were used to identify the optical, structural, and chemical aspects of the formed Cu-PANI nanocomposite. The antimicrobial activity of this nanocomposite shows that the microbial growth has been fully inhibited; moreover, some of the tested microbes were killed. Atomic force microscopy revealed dramatic changes in morphology of tested cells due to disruption of their cell wall integrity after incubation with Cu-PANI nanocomposite.",
journal = "ACS Applied Materials and Interfaces",
title = "Nanomaterial with High Antimicrobial Efficacy-Copper/Polyaniline Nanocomposite",
volume = "7",
number = "3",
pages = "1955-1966",
doi = "10.1021/am507746m"
}

Bogdanović, U., Vodnik, V., Mitrić, M., Dimitrijević, S. I., Škapin, S. D., Zunic, V., Budimir, M.,& Stoiljković, M.. (2015). Nanomaterial with High Antimicrobial Efficacy-Copper/Polyaniline Nanocomposite. in ACS Applied Materials and Interfaces, 7(3), 1955-1966.
https://doi.org/10.1021/am507746m

Bogdanović U, Vodnik V, Mitrić M, Dimitrijević SI, Škapin SD, Zunic V, Budimir M, Stoiljković M. Nanomaterial with High Antimicrobial Efficacy-Copper/Polyaniline Nanocomposite. in ACS Applied Materials and Interfaces. 2015;7(3):1955-1966.
doi:10.1021/am507746m .

Bogdanović, Una, Vodnik, Vesna, Mitrić, Miodrag, Dimitrijević, Suzana I., Škapin, Srečo Davor, Zunic, Vojka, Budimir, Milica, Stoiljković, Milovan, "Nanomaterial with High Antimicrobial Efficacy-Copper/Polyaniline Nanocomposite" in ACS Applied Materials and Interfaces, 7, no. 3 (2015):1955-1966,
https://doi.org/10.1021/am507746m . .

TY  - JOUR
AU  - Bogdanović, Una
AU  - Lazić, Vesna M.
AU  - Vodnik, Vesna
AU  - Budimir, Milica
AU  - Marković, Zoran M.
AU  - Dimitrijević, Suzana I.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6044
AB  - Copper (Cu) hydrosol containing small nanoparticles (NPs) (similar to 5 nm) with narrow size distribution were used to investigate antimicrobial activity toward representative microorganisms of public concern (Escherichia coli, Staphylococcus aureus and Candida albicans). Microbial reduction measurements were performed as a function of CuNPs concentration, after 2 h of their contact. Surface and morphological alterations of the strains exposed to the Cu NPs were studied on the cellular level using an atomic force microscope (AFM). (C) 2014 Elsevier B.V. All rights reserved.
T2  - Materials Letters
T1  - Copper nanoparticles with high antimicrobial activity
VL  - 128
SP  - 75
EP  - 78
DO  - 10.1016/j.matlet.2014.04.106
ER  - 

@article{
author = "Bogdanović, Una and Lazić, Vesna M. and Vodnik, Vesna and Budimir, Milica and Marković, Zoran M. and Dimitrijević, Suzana I.",
year = "2014",
abstract = "Copper (Cu) hydrosol containing small nanoparticles (NPs) (similar to 5 nm) with narrow size distribution were used to investigate antimicrobial activity toward representative microorganisms of public concern (Escherichia coli, Staphylococcus aureus and Candida albicans). Microbial reduction measurements were performed as a function of CuNPs concentration, after 2 h of their contact. Surface and morphological alterations of the strains exposed to the Cu NPs were studied on the cellular level using an atomic force microscope (AFM). (C) 2014 Elsevier B.V. All rights reserved.",
journal = "Materials Letters",
title = "Copper nanoparticles with high antimicrobial activity",
volume = "128",
pages = "75-78",
doi = "10.1016/j.matlet.2014.04.106"
}

Bogdanović, U., Lazić, V. M., Vodnik, V., Budimir, M., Marković, Z. M.,& Dimitrijević, S. I.. (2014). Copper nanoparticles with high antimicrobial activity. in Materials Letters, 128, 75-78.
https://doi.org/10.1016/j.matlet.2014.04.106

Bogdanović U, Lazić VM, Vodnik V, Budimir M, Marković ZM, Dimitrijević SI. Copper nanoparticles with high antimicrobial activity. in Materials Letters. 2014;128:75-78.
doi:10.1016/j.matlet.2014.04.106 .

Bogdanović, Una, Lazić, Vesna M., Vodnik, Vesna, Budimir, Milica, Marković, Zoran M., Dimitrijević, Suzana I., "Copper nanoparticles with high antimicrobial activity" in Materials Letters, 128 (2014):75-78,
https://doi.org/10.1016/j.matlet.2014.04.106 . .

TY  - CONF
AU  - Bogdanović, Una
AU  - Lazić, Vesna M.
AU  - Vodnik, Vesna
AU  - Budimir, Milica
AU  - Marković, Zoran M.
AU  - Dimitrijević, Suzana I.
AU  - Džunuzović, Jasna V.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9194
AB  - Copper nanoparticles (CuNPs), with an average particle size of about 5 nm,
was prepared by the simple chemical reduction procedure. TEM and UV–
Vis spectroscopy contributed to the analysis of size and optical properties of
CuNPs, and their antibacterial activity was evaluated toward human
pathogenic bacteria Escherichia coli and Staphylococcus aureus, in a
concentration dependent manner. The changes in the cell membrane
morphology of tested strains were investigated by atomic force microscope
(AFM), after 2 h of their contact with CuNPs. It was found that CuNPs
cause different types of cell membrane disruptions, as well as that S. aureus
bacteria were slightly resistant to the cell membrane damage than E. coli.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Antibacterial activity of copper nanoparticles
VL  - F-21-P
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9194
ER  - 

@conference{
author = "Bogdanović, Una and Lazić, Vesna M. and Vodnik, Vesna and Budimir, Milica and Marković, Zoran M. and Dimitrijević, Suzana I. and Džunuzović, Jasna V.",
year = "2014",
abstract = "Copper nanoparticles (CuNPs), with an average particle size of about 5 nm,
was prepared by the simple chemical reduction procedure. TEM and UV–
Vis spectroscopy contributed to the analysis of size and optical properties of
CuNPs, and their antibacterial activity was evaluated toward human
pathogenic bacteria Escherichia coli and Staphylococcus aureus, in a
concentration dependent manner. The changes in the cell membrane
morphology of tested strains were investigated by atomic force microscope
(AFM), after 2 h of their contact with CuNPs. It was found that CuNPs
cause different types of cell membrane disruptions, as well as that S. aureus
bacteria were slightly resistant to the cell membrane damage than E. coli.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Antibacterial activity of copper nanoparticles",
volume = "F-21-P",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9194"
}

Bogdanović, U., Lazić, V. M., Vodnik, V., Budimir, M., Marković, Z. M., Dimitrijević, S. I.,& Džunuzović, J. V.. (2014). Antibacterial activity of copper nanoparticles. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., F-21-P.
https://hdl.handle.net/21.15107/rcub_vinar_9194

Bogdanović U, Lazić VM, Vodnik V, Budimir M, Marković ZM, Dimitrijević SI, Džunuzović JV. Antibacterial activity of copper nanoparticles. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2014;F-21-P.
https://hdl.handle.net/21.15107/rcub_vinar_9194 .

Bogdanović, Una, Lazić, Vesna M., Vodnik, Vesna, Budimir, Milica, Marković, Zoran M., Dimitrijević, Suzana I., Džunuzović, Jasna V., "Antibacterial activity of copper nanoparticles" in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry, F-21-P (2014),
https://hdl.handle.net/21.15107/rcub_vinar_9194 .

TY  - JOUR
AU  - Stanić, Vojislav
AU  - Dimitrijević, Suzana I.
AU  - Antonović, Dušan
AU  - Jokić, Bojan M.
AU  - Zec, Slavica
AU  - Tanaskovic, Sladjana T.
AU  - Raičević, Slavica
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5820
AB  - Synthetic biomaterials based on fluorine substituted hydroxyapatite are potentially attractive for orthopedic and dental implant applications. The new synthesis of fluorine substituted hydroxyapatite samples were done by neutralization, which consists of adding the solution of HF and H3PO4 in suspension of Ca(OH)(2). Characterization studies from XRD, SEM and FTIR spectra showed that crystals are obtained with apatite structure and those particles of all samples are nano size, with an average length of 80 nm and about 15-25 nm in diameter. The central composite design was used in order to determine the optimal conditions for the antimicrobial activity of the synthesized samples. In order to evaluate the influence of operating parameters on the percent of viable cell reduction of Streptococcus mutans, three independent variables were chosen: exposure time, pH of saline and floride concentration in apatite samples. The experimental and predicted antimicrobial activities were in close agreement. Antimicrobial activity of the samples increases with the increase of fluoride concentration and the decreased pH of saline. The maximum antimicrobial activity was achieved at the initial pH of 4. (C) 2013 Elsevier B.V. All rights reserved.
T2  - Applied Surface Science
T1  - Synthesis of fluorine substituted hydroxyapatite nanopowders and application of the central composite design for determination of its antimicrobial effects
VL  - 290
SP  - 346
EP  - 352
DO  - 10.1016/j.apsusc.2013.11.081
ER  - 

@article{
author = "Stanić, Vojislav and Dimitrijević, Suzana I. and Antonović, Dušan and Jokić, Bojan M. and Zec, Slavica and Tanaskovic, Sladjana T. and Raičević, Slavica",
year = "2014",
abstract = "Synthetic biomaterials based on fluorine substituted hydroxyapatite are potentially attractive for orthopedic and dental implant applications. The new synthesis of fluorine substituted hydroxyapatite samples were done by neutralization, which consists of adding the solution of HF and H3PO4 in suspension of Ca(OH)(2). Characterization studies from XRD, SEM and FTIR spectra showed that crystals are obtained with apatite structure and those particles of all samples are nano size, with an average length of 80 nm and about 15-25 nm in diameter. The central composite design was used in order to determine the optimal conditions for the antimicrobial activity of the synthesized samples. In order to evaluate the influence of operating parameters on the percent of viable cell reduction of Streptococcus mutans, three independent variables were chosen: exposure time, pH of saline and floride concentration in apatite samples. The experimental and predicted antimicrobial activities were in close agreement. Antimicrobial activity of the samples increases with the increase of fluoride concentration and the decreased pH of saline. The maximum antimicrobial activity was achieved at the initial pH of 4. (C) 2013 Elsevier B.V. All rights reserved.",
journal = "Applied Surface Science",
title = "Synthesis of fluorine substituted hydroxyapatite nanopowders and application of the central composite design for determination of its antimicrobial effects",
volume = "290",
pages = "346-352",
doi = "10.1016/j.apsusc.2013.11.081"
}

Stanić, V., Dimitrijević, S. I., Antonović, D., Jokić, B. M., Zec, S., Tanaskovic, S. T.,& Raičević, S.. (2014). Synthesis of fluorine substituted hydroxyapatite nanopowders and application of the central composite design for determination of its antimicrobial effects. in Applied Surface Science, 290, 346-352.
https://doi.org/10.1016/j.apsusc.2013.11.081

Stanić V, Dimitrijević SI, Antonović D, Jokić BM, Zec S, Tanaskovic ST, Raičević S. Synthesis of fluorine substituted hydroxyapatite nanopowders and application of the central composite design for determination of its antimicrobial effects. in Applied Surface Science. 2014;290:346-352.
doi:10.1016/j.apsusc.2013.11.081 .

Stanić, Vojislav, Dimitrijević, Suzana I., Antonović, Dušan, Jokić, Bojan M., Zec, Slavica, Tanaskovic, Sladjana T., Raičević, Slavica, "Synthesis of fluorine substituted hydroxyapatite nanopowders and application of the central composite design for determination of its antimicrobial effects" in Applied Surface Science, 290 (2014):346-352,
https://doi.org/10.1016/j.apsusc.2013.11.081 . .