TY  - JOUR
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Božanić, Dušan K.
AU  - Bogdanović, Una
AU  - Vodnik, Vesna
AU  - Dimitrijević-Branković, Suzana I.
AU  - Miljković, Miona G.
AU  - Kaščakova, Slavka
AU  - Refregiers, Matthieu
AU  - Đoković, Vladimir
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1608
AB  - The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353 nm] and [370-410 nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells surfaces. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study
VL  - 155
SP  - 341
EP  - 348
DO  - 10.1016/j.colsurfb.2017.04.044
ER  - 

@article{
author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Bogdanović, Una and Vodnik, Vesna and Dimitrijević-Branković, Suzana I. and Miljković, Miona G. and Kaščakova, Slavka and Refregiers, Matthieu and Đoković, Vladimir",
year = "2017",
abstract = "The interaction of the tryptophan functionalized Ag nanoparticles and live Candida albicans cells was studied by synchrotron excitation deep-ultraviolet (DUV) fluorescence imaging at the DISCO beamline of Synchrotron SOLEIL. DUV imaging showed that incubation of the fungus with functionalized nanoparticles results in significant increase in the fluorescence signal. The analysis of the images revealed that the interaction of the nanoparticles with (pseudo)hyphae polymorphs of the diploid fungus was less pronounced than in the case of yeast cells or budding spores. The changes in the intensity of the fluorescence signals of the cells after incubation were followed in [327-353 nm] and [370-410 nm] spectral ranges that correspond to the fluorescence of tryptophan in non-polar and polar environment, respectively. As a consequence of the environmental sensitivity of the silver-tryptophan fluorescent nanoprobe, we were able to determine the possible accumulation sites of the nanoparticles. The analysis of the intensity decay kinetics showed that the photobleaching effects were more pronounced in the case of the functionalized nanoparticle treated cells. The results of time-integrated emission in the mentioned spectral ranges suggested that the nanoparticles penetrate the cells, but that the majority of the nanoparticles attach to the cells surfaces. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study",
volume = "155",
pages = "341-348",
doi = "10.1016/j.colsurfb.2017.04.044"
}

Dojčilović, R., Pajović, J. D., Božanić, D. K., Bogdanović, U., Vodnik, V., Dimitrijević-Branković, S. I., Miljković, M. G., Kaščakova, S., Refregiers, M.,& Đoković, V.. (2017). Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study. in Colloids and Surfaces. B: Biointerfaces, 155, 341-348.
https://doi.org/10.1016/j.colsurfb.2017.04.044

Dojčilović R, Pajović JD, Božanić DK, Bogdanović U, Vodnik V, Dimitrijević-Branković SI, Miljković MG, Kaščakova S, Refregiers M, Đoković V. Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study. in Colloids and Surfaces. B: Biointerfaces. 2017;155:341-348.
doi:10.1016/j.colsurfb.2017.04.044 .

Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Bogdanović, Una, Vodnik, Vesna, Dimitrijević-Branković, Suzana I., Miljković, Miona G., Kaščakova, Slavka, Refregiers, Matthieu, Đoković, Vladimir, "Interaction of amino acid-functionalized silver nanoparticles and Candida albicans polymorphs: A deep-UV fluorescence imaging study" in Colloids and Surfaces. B: Biointerfaces, 155 (2017):341-348,
https://doi.org/10.1016/j.colsurfb.2017.04.044 . .