TY  - JOUR
AU  - Matijević, Milica
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Radoičić, Marija B.
AU  - Liang, Xinyue
AU  - Mi, Lan
AU  - Filipović Tričković, Jelena G.
AU  - Valenta-Šobot, Ana
AU  - Stanković, Maja N.
AU  - Nakarada, Đura
AU  - Mojović, Miloš
AU  - Petković, Marijana
AU  - Stepić, Milutin
AU  - Nešić, Maja D.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9908
AB  - In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy.
T2  - Photochemical and Photobiological Sciences
T1  - Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles
VL  - 20
IS  - 8
SP  - 1087
EP  - 1098
DO  - 10.1007/s43630-021-00082-2
ER  - 

@article{
author = "Matijević, Milica and Žakula, Jelena and Korićanac, Lela and Radoičić, Marija B. and Liang, Xinyue and Mi, Lan and Filipović Tričković, Jelena G. and Valenta-Šobot, Ana and Stanković, Maja N. and Nakarada, Đura and Mojović, Miloš and Petković, Marijana and Stepić, Milutin and Nešić, Maja D.",
year = "2021",
abstract = "In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy.",
journal = "Photochemical and Photobiological Sciences",
title = "Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles",
volume = "20",
number = "8",
pages = "1087-1098",
doi = "10.1007/s43630-021-00082-2"
}

Matijević, M., Žakula, J., Korićanac, L., Radoičić, M. B., Liang, X., Mi, L., Filipović Tričković, J. G., Valenta-Šobot, A., Stanković, M. N., Nakarada, Đ., Mojović, M., Petković, M., Stepić, M.,& Nešić, M. D.. (2021). Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles. in Photochemical and Photobiological Sciences, 20(8), 1087-1098.
https://doi.org/10.1007/s43630-021-00082-2

Matijević M, Žakula J, Korićanac L, Radoičić MB, Liang X, Mi L, Filipović Tričković JG, Valenta-Šobot A, Stanković MN, Nakarada Đ, Mojović M, Petković M, Stepić M, Nešić MD. Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles. in Photochemical and Photobiological Sciences. 2021;20(8):1087-1098.
doi:10.1007/s43630-021-00082-2 .

Matijević, Milica, Žakula, Jelena, Korićanac, Lela, Radoičić, Marija B., Liang, Xinyue, Mi, Lan, Filipović Tričković, Jelena G., Valenta-Šobot, Ana, Stanković, Maja N., Nakarada, Đura, Mojović, Miloš, Petković, Marijana, Stepić, Milutin, Nešić, Maja D., "Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles" in Photochemical and Photobiological Sciences, 20, no. 8 (2021):1087-1098,
https://doi.org/10.1007/s43630-021-00082-2 . .