TY  - JOUR
AU  - Jakovljević-Uzelac, Jovana
AU  - Stanić, Marina
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s11010-017-3238-z
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7788
AB  - The objective of this study was to investigate in vitro effects of 10 A mu M dl-homocysteine (dl-Hcy), dl-homocysteine thiolactone-hydrochloride (dl-Hcy TLHC), and l-homocysteine thiolactone-hydrochloride (l-Hcy TLHC) on the oxygen consumption of rat heart tissue homogenate, as well as the involvement of the gasotransmitters NO, H2S and CO in the effects of the most toxic homocysteine compound, dl-Hcy TLHC. The possible contribution of the gasotransmitters in these effects was estimated by using the appropriate inhibitors of their synthesis (N (omega)-nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (dl-PAG), and zinc protoporphyrin IX (ZnPPR IX), respectively). The oxygen consumption of rat heart tissue homogenate was measured by Clark/type oxygen electrode in the absence and presence of the investigated compounds. All three homocysteine-based compounds caused a similar decrease in the oxygen consumption rate compared to control: 15.19 +/- 4.01%, 12.42 +/- 1.01%, and 16.43 +/- 4.52% for dl-Hcy, dl-Hcy TLHC, or l-Hcy TLHC, respectively. All applied inhibitors of gasotransmitter synthesis also decreased the oxygen consumption rate of tissue homogenate related to control: 13.53 +/- 1.35% for l-NAME (30 A mu M), 5.32 +/- 1.23% for dl-PAG (10 A mu M), and 5.56 +/- 1.39% for ZnPPR IX (10 A mu M). Simultaneous effect of l-NAME (30 A mu M) or ZnPPR IX (10 A mu M) with dl-Hcy TLHC (10 A mu M) caused a larger decrease of oxygen consumption compared to each of the substances individually. However, when dl-PAG (10 A mu M) was applied together with dl-Hcy TLHC (10 A mu M), it attenuated the effect of dl-Hcy TLHC from 12.42 +/- 1.01 to 9.22 +/- 1.58%. In conclusion, cardiotoxicity induced by Hcy-related compounds, which was shown in our previous research, could result from the inhibition of the oxygen consumption, and might be mediated by the certain gasotransmitters.
T2  - Molecular and Cellular Biochemistry
T1  - Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters
VL  - 444
IS  - 1-2
SP  - 143
EP  - 148
DO  - 10.1007/s11010-017-3238-z
ER  - 

@article{
author = "Jakovljević-Uzelac, Jovana and Stanić, Marina and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Dragan M.",
year = "2018",
abstract = "The objective of this study was to investigate in vitro effects of 10 A mu M dl-homocysteine (dl-Hcy), dl-homocysteine thiolactone-hydrochloride (dl-Hcy TLHC), and l-homocysteine thiolactone-hydrochloride (l-Hcy TLHC) on the oxygen consumption of rat heart tissue homogenate, as well as the involvement of the gasotransmitters NO, H2S and CO in the effects of the most toxic homocysteine compound, dl-Hcy TLHC. The possible contribution of the gasotransmitters in these effects was estimated by using the appropriate inhibitors of their synthesis (N (omega)-nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (dl-PAG), and zinc protoporphyrin IX (ZnPPR IX), respectively). The oxygen consumption of rat heart tissue homogenate was measured by Clark/type oxygen electrode in the absence and presence of the investigated compounds. All three homocysteine-based compounds caused a similar decrease in the oxygen consumption rate compared to control: 15.19 +/- 4.01%, 12.42 +/- 1.01%, and 16.43 +/- 4.52% for dl-Hcy, dl-Hcy TLHC, or l-Hcy TLHC, respectively. All applied inhibitors of gasotransmitter synthesis also decreased the oxygen consumption rate of tissue homogenate related to control: 13.53 +/- 1.35% for l-NAME (30 A mu M), 5.32 +/- 1.23% for dl-PAG (10 A mu M), and 5.56 +/- 1.39% for ZnPPR IX (10 A mu M). Simultaneous effect of l-NAME (30 A mu M) or ZnPPR IX (10 A mu M) with dl-Hcy TLHC (10 A mu M) caused a larger decrease of oxygen consumption compared to each of the substances individually. However, when dl-PAG (10 A mu M) was applied together with dl-Hcy TLHC (10 A mu M), it attenuated the effect of dl-Hcy TLHC from 12.42 +/- 1.01 to 9.22 +/- 1.58%. In conclusion, cardiotoxicity induced by Hcy-related compounds, which was shown in our previous research, could result from the inhibition of the oxygen consumption, and might be mediated by the certain gasotransmitters.",
journal = "Molecular and Cellular Biochemistry",
title = "Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters",
volume = "444",
number = "1-2",
pages = "143-148",
doi = "10.1007/s11010-017-3238-z"
}

Jakovljević-Uzelac, J., Stanić, M., Krstić, D. Z., Čolović, M. B.,& Đurić, D. M.. (2018). Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters. in Molecular and Cellular Biochemistry, 444(1-2), 143-148.
https://doi.org/10.1007/s11010-017-3238-z

Jakovljević-Uzelac J, Stanić M, Krstić DZ, Čolović MB, Đurić DM. Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters. in Molecular and Cellular Biochemistry. 2018;444(1-2):143-148.
doi:10.1007/s11010-017-3238-z .

Jakovljević-Uzelac, Jovana, Stanić, Marina, Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Dragan M., "Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters" in Molecular and Cellular Biochemistry, 444, no. 1-2 (2018):143-148,
https://doi.org/10.1007/s11010-017-3238-z . .

TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Spasojević, Dragica
AU  - Orlovska, Irina V.
AU  - Kozyrovska, Natalia O.
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Dmitrović, Svetlana
AU  - Matović, Branko
AU  - Tasić, Nikola B.
AU  - Maksimović, Vuk M.
AU  - Sosnin, Mikhail
AU  - Radotić, Ksenija
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7769
AB  - Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.
T2  - International Journal of Biological Macromolecules
T1  - Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing
VL  - 118
SP  - 494
EP  - 503
DO  - 10.1016/j.ijbiomac.2018.06.067
ER  - 

@article{
author = "Zmejkoski, Danica and Spasojević, Dragica and Orlovska, Irina V. and Kozyrovska, Natalia O. and Soković, Marina and Glamočlija, Jasmina and Dmitrović, Svetlana and Matović, Branko and Tasić, Nikola B. and Maksimović, Vuk M. and Sosnin, Mikhail and Radotić, Ksenija",
year = "2018",
abstract = "Lignins and lignin-derived compounds are known to have antibacterial properties. The wound healing agents in the form of dressings produce faster skin repair and decrease pain in patients. In order to create an efficient antimicrobial agent in the form of dressing in the treatment of chronic wounds, a composite hydrogel of bacterial cellulose (BC) and dehydrogenative polymer of coniferyl alcohol (DHP), BC-DHP, was designed. Novel composite showed inhibitory or bactericidal effects against selected pathogenic bacteria, including clinically isolated ones. The highest release rate of DHP was in the first hour, while after 24 h there was still slow release of small amounts of DHP from BC-DHP during 72 h monitoring. High-performance liquid chromatography coupled with mass-spectrometry showed that BC-DHP releases DHP oligomers, which are proposed to be antimicrobially active DHP fractions. Scanning electron microscopy and atomic force microscopy micrographs proved a dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number and size in the cellulose membrane. The Fourier-transform infrared absorption spectra of the BC-DHP showed that DHP was partly bound to the BC matrix. The swelling and crystallinity degree were dose-dependent. All obtained results confirmed BC-DHP composite as a promising hydrogel for wounds healing.",
journal = "International Journal of Biological Macromolecules",
title = "Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing",
volume = "118",
pages = "494-503",
doi = "10.1016/j.ijbiomac.2018.06.067"
}

Zmejkoski, D., Spasojević, D., Orlovska, I. V., Kozyrovska, N. O., Soković, M., Glamočlija, J., Dmitrović, S., Matović, B., Tasić, N. B., Maksimović, V. M., Sosnin, M.,& Radotić, K.. (2018). Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing. in International Journal of Biological Macromolecules, 118, 494-503.
https://doi.org/10.1016/j.ijbiomac.2018.06.067

Zmejkoski D, Spasojević D, Orlovska IV, Kozyrovska NO, Soković M, Glamočlija J, Dmitrović S, Matović B, Tasić NB, Maksimović VM, Sosnin M, Radotić K. Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing. in International Journal of Biological Macromolecules. 2018;118:494-503.
doi:10.1016/j.ijbiomac.2018.06.067 .

Zmejkoski, Danica, Spasojević, Dragica, Orlovska, Irina V., Kozyrovska, Natalia O., Soković, Marina, Glamočlija, Jasmina, Dmitrović, Svetlana, Matović, Branko, Tasić, Nikola B., Maksimović, Vuk M., Sosnin, Mikhail, Radotić, Ksenija, "Bacterial cellulose-lignin composite hydrogel as a promising agent in chronic wound healing" in International Journal of Biological Macromolecules, 118 (2018):494-503,
https://doi.org/10.1016/j.ijbiomac.2018.06.067 . .

TY  - CONF
AU  - Pejin, B.
AU  - Matović, Branko
AU  - Nikolić, M.
AU  - Hegediš, A.
AU  - Karaman, I.
AU  - Mutavdžić, D.
AU  - Savić, A.
AU  - Horvatović, M.
AU  - Radotić, K.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9303
AB  - Structure of the lyophilised colonies of the freshwater bryozoan Hyalinella
punctata (Hancock, 1850) and their mineral element composition were studied by
X ray diffraction, scanning electron microscopy (SEM) and inductively coupled
plasma (ICP) spectrometry for the first time ever. The X ray measurements showed
amorphous structure of the bryozoan, while SEM indicated porous connected
structure without regular structural motifs. Considerable presence of phosphorus
(7949.69 μg/ml), sulphur (6204.7 μg/ml) and calcium (6139.58 μg/ml), as well as
low heavy metal content was found by ICP. The obtained results jointly indicate
the potential of the investigated animal species as a base for bioscaffolds and
nanocomposites
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
T1  - Structure and mineral element composition of the lyophilised freshwater bryozoan Hyallinela punctata
VL  - 1
SP  - 403
EP  - 405
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9303
ER  - 

@conference{
author = "Pejin, B. and Matović, Branko and Nikolić, M. and Hegediš, A. and Karaman, I. and Mutavdžić, D. and Savić, A. and Horvatović, M. and Radotić, K.",
year = "2012",
abstract = "Structure of the lyophilised colonies of the freshwater bryozoan Hyalinella
punctata (Hancock, 1850) and their mineral element composition were studied by
X ray diffraction, scanning electron microscopy (SEM) and inductively coupled
plasma (ICP) spectrometry for the first time ever. The X ray measurements showed
amorphous structure of the bryozoan, while SEM indicated porous connected
structure without regular structural motifs. Considerable presence of phosphorus
(7949.69 μg/ml), sulphur (6204.7 μg/ml) and calcium (6139.58 μg/ml), as well as
low heavy metal content was found by ICP. The obtained results jointly indicate
the potential of the investigated animal species as a base for bioscaffolds and
nanocomposites",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry",
title = "Structure and mineral element composition of the lyophilised freshwater bryozoan Hyallinela punctata",
volume = "1",
pages = "403-405",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9303"
}

Pejin, B., Matović, B., Nikolić, M., Hegediš, A., Karaman, I., Mutavdžić, D., Savić, A., Horvatović, M.,& Radotić, K.. (2012). Structure and mineral element composition of the lyophilised freshwater bryozoan Hyallinela punctata. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 403-405.
https://hdl.handle.net/21.15107/rcub_vinar_9303

Pejin B, Matović B, Nikolić M, Hegediš A, Karaman I, Mutavdžić D, Savić A, Horvatović M, Radotić K. Structure and mineral element composition of the lyophilised freshwater bryozoan Hyallinela punctata. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry. 2012;1:403-405.
https://hdl.handle.net/21.15107/rcub_vinar_9303 .

Pejin, B., Matović, Branko, Nikolić, M., Hegediš, A., Karaman, I., Mutavdžić, D., Savić, A., Horvatović, M., Radotić, K., "Structure and mineral element composition of the lyophilised freshwater bryozoan Hyallinela punctata" in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry, 1 (2012):403-405,
https://hdl.handle.net/21.15107/rcub_vinar_9303 .