TY  - JOUR
AU  - Petrović, Marija
AU  - Popović, Suzana
AU  - Baskić, Dejan
AU  - Todorović, Miloš
AU  - Đurđević, Predrag
AU  - Ristić-Fira, Aleksandra
AU  - Keta, Otilija D.
AU  - Petković, Vladana
AU  - Korićanac, Lela
AU  - Stojković, Danijela
AU  - Jevtić, Verica
AU  - Trifunović, Srećko
AU  - Todorović, Danijela
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9631
AB  - Aim: Newly synthesized platinum(IV) complexes with ethylenediamine-N,N’-diacetate ligands (EDDA-type) (butyl-Pt and pentyl-Pt) were investigated against two cancer (A549 lung, and HTB 140 melanoma) and one non-cancerous (MRC-5 embryonic lung fibroblast) human cell lines. Materials and Methods: The effects of these agents were compared with those of cisplatin after 6-, 24- and 48-h treatment. Sulforhodamine-B (SRB) assay was performed to estimate the cytotoxic effect, while the inhibitory effect on cell proliferation was measured using 5-bromo-2,-deoxyuridine (BrdU) incorporation assay. Cell cycle analysis was performed by flow cytometry. Type of cell death induced by these agents was determined by electrophoretic analysis of DNA, flow cytometry and by western blot analysis of proteins involved in induction of apoptosis. The effects of gamma irradiation, alone and in combination with platinum-based compounds, were examined by clonogenic and SRB assays. Results: All examined platinum-based compounds had inhibitory and antiproliferative effects on A549 cells, but not on HTB140 and MRC-5 cells. Butyl-Pt, pentyl-Pt and cisplatin arrested the cell cycle in the S-phase and induced apoptotic cell death via regulation of expression of B-cell lymphoma 2 (BCL2) and BCL2-associated X (BAX) proteins. Platinum-based compounds increased the sensitivity of A549 cells to gamma irradiation. Butyl-Pt and pentyl-Pt showed better antitumour effects against A549 cells than did cisplatin, by interfering in cell proliferation and the cell cycle, and by triggering apoptosis. Conclusion: The effects of gamma irradiation on tumour cells may be amplified by pre-treatment of cells with platinum-based compounds.
T2  - Anticancer Research
T1  - The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro
VL  - 40
IS  - 9
SP  - 5001
EP  - 5013
DO  - 10.21873/anticanres.14503
ER  - 

@article{
author = "Petrović, Marija and Popović, Suzana and Baskić, Dejan and Todorović, Miloš and Đurđević, Predrag and Ristić-Fira, Aleksandra and Keta, Otilija D. and Petković, Vladana and Korićanac, Lela and Stojković, Danijela and Jevtić, Verica and Trifunović, Srećko and Todorović, Danijela",
year = "2020",
abstract = "Aim: Newly synthesized platinum(IV) complexes with ethylenediamine-N,N’-diacetate ligands (EDDA-type) (butyl-Pt and pentyl-Pt) were investigated against two cancer (A549 lung, and HTB 140 melanoma) and one non-cancerous (MRC-5 embryonic lung fibroblast) human cell lines. Materials and Methods: The effects of these agents were compared with those of cisplatin after 6-, 24- and 48-h treatment. Sulforhodamine-B (SRB) assay was performed to estimate the cytotoxic effect, while the inhibitory effect on cell proliferation was measured using 5-bromo-2,-deoxyuridine (BrdU) incorporation assay. Cell cycle analysis was performed by flow cytometry. Type of cell death induced by these agents was determined by electrophoretic analysis of DNA, flow cytometry and by western blot analysis of proteins involved in induction of apoptosis. The effects of gamma irradiation, alone and in combination with platinum-based compounds, were examined by clonogenic and SRB assays. Results: All examined platinum-based compounds had inhibitory and antiproliferative effects on A549 cells, but not on HTB140 and MRC-5 cells. Butyl-Pt, pentyl-Pt and cisplatin arrested the cell cycle in the S-phase and induced apoptotic cell death via regulation of expression of B-cell lymphoma 2 (BCL2) and BCL2-associated X (BAX) proteins. Platinum-based compounds increased the sensitivity of A549 cells to gamma irradiation. Butyl-Pt and pentyl-Pt showed better antitumour effects against A549 cells than did cisplatin, by interfering in cell proliferation and the cell cycle, and by triggering apoptosis. Conclusion: The effects of gamma irradiation on tumour cells may be amplified by pre-treatment of cells with platinum-based compounds.",
journal = "Anticancer Research",
title = "The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro",
volume = "40",
number = "9",
pages = "5001-5013",
doi = "10.21873/anticanres.14503"
}

Petrović, M., Popović, S., Baskić, D., Todorović, M., Đurđević, P., Ristić-Fira, A., Keta, O. D., Petković, V., Korićanac, L., Stojković, D., Jevtić, V., Trifunović, S.,& Todorović, D.. (2020). The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro. in Anticancer Research, 40(9), 5001-5013.
https://doi.org/10.21873/anticanres.14503

Petrović M, Popović S, Baskić D, Todorović M, Đurđević P, Ristić-Fira A, Keta OD, Petković V, Korićanac L, Stojković D, Jevtić V, Trifunović S, Todorović D. The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro. in Anticancer Research. 2020;40(9):5001-5013.
doi:10.21873/anticanres.14503 .

Petrović, Marija, Popović, Suzana, Baskić, Dejan, Todorović, Miloš, Đurđević, Predrag, Ristić-Fira, Aleksandra, Keta, Otilija D., Petković, Vladana, Korićanac, Lela, Stojković, Danijela, Jevtić, Verica, Trifunović, Srećko, Todorović, Danijela, "The Effects of Newly Synthesized Platinum(IV) Complexes on Cytotoxicity and Radiosensitization of Human Tumour Cells In Vitro" in Anticancer Research, 40, no. 9 (2020):5001-5013,
https://doi.org/10.21873/anticanres.14503 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Filipović Tričković, Jelena G.
AU  - Mićić, Mileva
AU  - Joksić, Ivana
AU  - Valenta-Šobot, Ana
AU  - Demajo, Miroslav
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9729
AB  - Traditional methods in cell proliferation studies are based on immunohistochemical detection of proliferating cells in the target tissue. Since they are time consuming, optimization of novel, more efficient methods is important for large scale proliferation studies. In this study, we aimed to optimize the isolation of single epithelial rat forestomach cells for flow cytometry. As a marker of cellular proliferation we used the Ki-67 antibody to detect this nuclear protein expressed in proliferating cells. We also performed immunohistochemical detection of Ki-67 positive cells and propidium iodide staining to validate the results. 3-tert-butyl-4-hydroxyanisole was used as the positive control to ensure cellular proliferation. The results showed that isolation of epithelial cells with collagenase, trypsin and cell strainer ensures great cell viability (>95%) and the purity of the samples. Flow cytometry and immunostaining with the Ki-67 antibody indicated that 3-tert-butyl-4-hydroxyanisole treatment leads to a significant increase in proliferation. A significant positive correlation was observed between the results obtained by immunohistochemistry and flow cytometry, but the flow cytometric data had a smaller measurement error, suggesting the equal sensitivity and greater accuracy of this method. Propidium iodide staining showed that the percentage of cells in the G2+S phase of the cell cycle correlated positively with the percentage of Ki-67 positive cells assessed by flow cytometry, indicating that Ki-67 positive cells reflect an active dividing cell pool. We conclude that the isolation of forestomach epithelial cells described is a simple and reliable method for obtaining viable cells for use in flow cytometry. Compared to immunohistochemistry, flow cytometric detection of the Ki-67 antigen is equally sensitive, but much faster and provides more accurate results. © 2020, University of Zagreb, Facultty of Veterinary Medicine. All rights reserved.
T2  - Veterinarski Arhiv
T1  - Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry
VL  - 90
IS  - 5
SP  - 517
EP  - 525
DO  - 10.24099/vet.arhiv.0956
ER  - 

@article{
author = "Joksić, Gordana and Filipović Tričković, Jelena G. and Mićić, Mileva and Joksić, Ivana and Valenta-Šobot, Ana and Demajo, Miroslav",
year = "2020",
abstract = "Traditional methods in cell proliferation studies are based on immunohistochemical detection of proliferating cells in the target tissue. Since they are time consuming, optimization of novel, more efficient methods is important for large scale proliferation studies. In this study, we aimed to optimize the isolation of single epithelial rat forestomach cells for flow cytometry. As a marker of cellular proliferation we used the Ki-67 antibody to detect this nuclear protein expressed in proliferating cells. We also performed immunohistochemical detection of Ki-67 positive cells and propidium iodide staining to validate the results. 3-tert-butyl-4-hydroxyanisole was used as the positive control to ensure cellular proliferation. The results showed that isolation of epithelial cells with collagenase, trypsin and cell strainer ensures great cell viability (>95%) and the purity of the samples. Flow cytometry and immunostaining with the Ki-67 antibody indicated that 3-tert-butyl-4-hydroxyanisole treatment leads to a significant increase in proliferation. A significant positive correlation was observed between the results obtained by immunohistochemistry and flow cytometry, but the flow cytometric data had a smaller measurement error, suggesting the equal sensitivity and greater accuracy of this method. Propidium iodide staining showed that the percentage of cells in the G2+S phase of the cell cycle correlated positively with the percentage of Ki-67 positive cells assessed by flow cytometry, indicating that Ki-67 positive cells reflect an active dividing cell pool. We conclude that the isolation of forestomach epithelial cells described is a simple and reliable method for obtaining viable cells for use in flow cytometry. Compared to immunohistochemistry, flow cytometric detection of the Ki-67 antigen is equally sensitive, but much faster and provides more accurate results. © 2020, University of Zagreb, Facultty of Veterinary Medicine. All rights reserved.",
journal = "Veterinarski Arhiv",
title = "Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry",
volume = "90",
number = "5",
pages = "517-525",
doi = "10.24099/vet.arhiv.0956"
}

Joksić, G., Filipović Tričković, J. G., Mićić, M., Joksić, I., Valenta-Šobot, A.,& Demajo, M.. (2020). Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry. in Veterinarski Arhiv, 90(5), 517-525.
https://doi.org/10.24099/vet.arhiv.0956

Joksić G, Filipović Tričković JG, Mićić M, Joksić I, Valenta-Šobot A, Demajo M. Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry. in Veterinarski Arhiv. 2020;90(5):517-525.
doi:10.24099/vet.arhiv.0956 .

Joksić, Gordana, Filipović Tričković, Jelena G., Mićić, Mileva, Joksić, Ivana, Valenta-Šobot, Ana, Demajo, Miroslav, "Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry" in Veterinarski Arhiv, 90, no. 5 (2020):517-525,
https://doi.org/10.24099/vet.arhiv.0956 . .

TY  - JOUR
AU  - Sakata, Dousatsu
AU  - Belov, Oleg
AU  - Bordage, Marie-Claude
AU  - Emfietzoglou, Dimitris
AU  - Guatelli, Susanna
AU  - Inaniwa, Taku
AU  - Ivanchenko, Vladimir
AU  - Karamitros, Mathieu
AU  - Kyriakou, Ioanna
AU  - Lampe, Nathanael
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Shin, Wook-Geun
AU  - Incerti, Sebastien
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9751
AB  - Ionising radiation induced DNA damage and subsequent biological responses to it depend on the radiation's track-structure and its energy loss distribution pattern. To investigate the underlying biological mechanisms involved in such complex system, there is need of predicting biological response by integrated Monte Carlo (MC) simulations across physics, chemistry and biology. Hence, in this work, we have developed an application using the open source Geant4-DNA toolkit to propose a realistic "fully integrated" MC simulation to calculate both early DNA damage and subsequent biological responses with time. We had previously developed an application allowing simulations of radiation induced early DNA damage on a naked cell nucleus model. In the new version presented in this work, we have developed three additional important features: (1) modeling of a realistic cell geometry, (2) inclusion of a biological repair model, (3) refinement of DNA damage parameters for direct damage and indirect damage scoring. The simulation results are validated with experimental data in terms of Single Strand Break (SSB) yields for plasmid and Double Strand Break (DSB) yields for plasmid/human cell. In addition, the yields of indirect DSBs are compatible with the experimental scavengeable damage fraction. The simulation application also demonstrates agreement with experimental data of gamma -H2AX yields for gamma ray irradiation. Using this application, it is now possible to predict biological response along time through track-structure MC simulations.
T2  - Scientific Reports
T1  - Fully integrated Monte Carlo simulation for evaluating radiation induced DNA damage and subsequent repair using Geant4-DNA
VL  - 10
IS  - 1
SP  - 20788
DO  - 10.1038/s41598-020-75982-x
ER  - 

@article{
author = "Sakata, Dousatsu and Belov, Oleg and Bordage, Marie-Claude and Emfietzoglou, Dimitris and Guatelli, Susanna and Inaniwa, Taku and Ivanchenko, Vladimir and Karamitros, Mathieu and Kyriakou, Ioanna and Lampe, Nathanael and Petrović, Ivan M. and Ristić-Fira, Aleksandra and Shin, Wook-Geun and Incerti, Sebastien",
year = "2020",
abstract = "Ionising radiation induced DNA damage and subsequent biological responses to it depend on the radiation's track-structure and its energy loss distribution pattern. To investigate the underlying biological mechanisms involved in such complex system, there is need of predicting biological response by integrated Monte Carlo (MC) simulations across physics, chemistry and biology. Hence, in this work, we have developed an application using the open source Geant4-DNA toolkit to propose a realistic "fully integrated" MC simulation to calculate both early DNA damage and subsequent biological responses with time. We had previously developed an application allowing simulations of radiation induced early DNA damage on a naked cell nucleus model. In the new version presented in this work, we have developed three additional important features: (1) modeling of a realistic cell geometry, (2) inclusion of a biological repair model, (3) refinement of DNA damage parameters for direct damage and indirect damage scoring. The simulation results are validated with experimental data in terms of Single Strand Break (SSB) yields for plasmid and Double Strand Break (DSB) yields for plasmid/human cell. In addition, the yields of indirect DSBs are compatible with the experimental scavengeable damage fraction. The simulation application also demonstrates agreement with experimental data of gamma -H2AX yields for gamma ray irradiation. Using this application, it is now possible to predict biological response along time through track-structure MC simulations.",
journal = "Scientific Reports",
title = "Fully integrated Monte Carlo simulation for evaluating radiation induced DNA damage and subsequent repair using Geant4-DNA",
volume = "10",
number = "1",
pages = "20788",
doi = "10.1038/s41598-020-75982-x"
}

Sakata, D., Belov, O., Bordage, M., Emfietzoglou, D., Guatelli, S., Inaniwa, T., Ivanchenko, V., Karamitros, M., Kyriakou, I., Lampe, N., Petrović, I. M., Ristić-Fira, A., Shin, W.,& Incerti, S.. (2020). Fully integrated Monte Carlo simulation for evaluating radiation induced DNA damage and subsequent repair using Geant4-DNA. in Scientific Reports, 10(1), 20788.
https://doi.org/10.1038/s41598-020-75982-x

Sakata D, Belov O, Bordage M, Emfietzoglou D, Guatelli S, Inaniwa T, Ivanchenko V, Karamitros M, Kyriakou I, Lampe N, Petrović IM, Ristić-Fira A, Shin W, Incerti S. Fully integrated Monte Carlo simulation for evaluating radiation induced DNA damage and subsequent repair using Geant4-DNA. in Scientific Reports. 2020;10(1):20788.
doi:10.1038/s41598-020-75982-x .

Sakata, Dousatsu, Belov, Oleg, Bordage, Marie-Claude, Emfietzoglou, Dimitris, Guatelli, Susanna, Inaniwa, Taku, Ivanchenko, Vladimir, Karamitros, Mathieu, Kyriakou, Ioanna, Lampe, Nathanael, Petrović, Ivan M., Ristić-Fira, Aleksandra, Shin, Wook-Geun, Incerti, Sebastien, "Fully integrated Monte Carlo simulation for evaluating radiation induced DNA damage and subsequent repair using Geant4-DNA" in Scientific Reports, 10, no. 1 (2020):20788,
https://doi.org/10.1038/s41598-020-75982-x . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Deljanin, Milena
AU  - Petković, Vladana
AU  - Zdunić, Gordana
AU  - Janković, Teodora
AU  - Živković, Jelena
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
AU  - Šavikin, Katarina
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8996
AB  - The aim of the present study was to investigate effects of pomegranate peel (PP) extract on different human cancer cell lines. MTT was performed to estimate cytotoxic effects of PP extract against HTB140, HTB177, MCF7, HCT116 human cancer cell lines and MRC-5 normal fibroblasts. Clonogenic assay was used to reveal cell survival after the treatment with PP extract. Cell cycle analysis was done using flow cytometry. Wound healing assay was applied to estimate inhibitory effects of PP extract on migration of cancer cells. The results showed that PP extract expressed selective cytotoxicity for cancer cells compared to normal cell line. Analyzed cancer cell lines displayed individual variations in sensitivity to PP extract reflected through changes in clonogenic survival, cell cycle distribution and migration, which may be due to the specific nature of each tested cell line. In conclusion, PP extract exhibits good inhibitory effects on tested cancer cell lines.
T2  - Records of Natural Products
T1  - Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines
VL  - 14
IS  - 5
SP  - 326
EP  - 339
DO  - 10.25135/rnp.170.19.11.1477
ER  - 

@article{
author = "Keta, Otilija D. and Deljanin, Milena and Petković, Vladana and Zdunić, Gordana and Janković, Teodora and Živković, Jelena and Ristić-Fira, Aleksandra and Petrović, Ivan M. and Šavikin, Katarina",
year = "2020",
abstract = "The aim of the present study was to investigate effects of pomegranate peel (PP) extract on different human cancer cell lines. MTT was performed to estimate cytotoxic effects of PP extract against HTB140, HTB177, MCF7, HCT116 human cancer cell lines and MRC-5 normal fibroblasts. Clonogenic assay was used to reveal cell survival after the treatment with PP extract. Cell cycle analysis was done using flow cytometry. Wound healing assay was applied to estimate inhibitory effects of PP extract on migration of cancer cells. The results showed that PP extract expressed selective cytotoxicity for cancer cells compared to normal cell line. Analyzed cancer cell lines displayed individual variations in sensitivity to PP extract reflected through changes in clonogenic survival, cell cycle distribution and migration, which may be due to the specific nature of each tested cell line. In conclusion, PP extract exhibits good inhibitory effects on tested cancer cell lines.",
journal = "Records of Natural Products",
title = "Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines",
volume = "14",
number = "5",
pages = "326-339",
doi = "10.25135/rnp.170.19.11.1477"
}

Keta, O. D., Deljanin, M., Petković, V., Zdunić, G., Janković, T., Živković, J., Ristić-Fira, A., Petrović, I. M.,& Šavikin, K.. (2020). Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines. in Records of Natural Products, 14(5), 326-339.
https://doi.org/10.25135/rnp.170.19.11.1477

Keta OD, Deljanin M, Petković V, Zdunić G, Janković T, Živković J, Ristić-Fira A, Petrović IM, Šavikin K. Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines. in Records of Natural Products. 2020;14(5):326-339.
doi:10.25135/rnp.170.19.11.1477 .

Keta, Otilija D., Deljanin, Milena, Petković, Vladana, Zdunić, Gordana, Janković, Teodora, Živković, Jelena, Ristić-Fira, Aleksandra, Petrović, Ivan M., Šavikin, Katarina, "Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent Against Different Cancer Cell Lines" in Records of Natural Products, 14, no. 5 (2020):326-339,
https://doi.org/10.25135/rnp.170.19.11.1477 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Valenta-Šobot, Ana
AU  - Filipović Tričković, Jelena G.
AU  - Maletić, Dejan
AU  - Puač, Nevena
AU  - Malović, Gordana N.
AU  - Petrović, Zoran Lj.
AU  - Lazović, Saša
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8178
AB  - In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4-1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of 2-3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h. © 2019, Indian Academy of Sciences.
T2  - Current Science
T1  - Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation
VL  - 116
IS  - 7
SP  - 1229
EP  - 1233
DO  - 10.18520/cs/v116/i7/1229-1233
ER  - 

@article{
author = "Joksić, Gordana and Valenta-Šobot, Ana and Filipović Tričković, Jelena G. and Maletić, Dejan and Puač, Nevena and Malović, Gordana N. and Petrović, Zoran Lj. and Lazović, Saša",
year = "2019",
abstract = "In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4-1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of 2-3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h. © 2019, Indian Academy of Sciences.",
journal = "Current Science",
title = "Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation",
volume = "116",
number = "7",
pages = "1229-1233",
doi = "10.18520/cs/v116/i7/1229-1233"
}

Joksić, G., Valenta-Šobot, A., Filipović Tričković, J. G., Maletić, D., Puač, N., Malović, G. N., Petrović, Z. Lj.,& Lazović, S.. (2019). Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation. in Current Science, 116(7), 1229-1233.
https://doi.org/10.18520/cs/v116/i7/1229-1233

Joksić G, Valenta-Šobot A, Filipović Tričković JG, Maletić D, Puač N, Malović GN, Petrović ZL, Lazović S. Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation. in Current Science. 2019;116(7):1229-1233.
doi:10.18520/cs/v116/i7/1229-1233 .

Joksić, Gordana, Valenta-Šobot, Ana, Filipović Tričković, Jelena G., Maletić, Dejan, Puač, Nevena, Malović, Gordana N., Petrović, Zoran Lj., Lazović, Saša, "Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation" in Current Science, 116, no. 7 (2019):1229-1233,
https://doi.org/10.18520/cs/v116/i7/1229-1233 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Filipović Tričković, Jelena G.
AU  - Joksić, Ivana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8516
AB  - Background: Obesity, diabetes, and associated diseases are increasing all over the world, and pose a great burden on public health. According to the latest reports, 440 million people are suffering from diabetes. Diabetes is caused by impaired ability to produce or respond to the hormone insulin consequently resulting in hyperglycemia. Methods: Data used for this review was obtained by using PUBMED/MEDLINE (1987-2018). The main data search terms were: Gentiana lutea, Gentiana lutea extract, Gentiana lutea constituents, obesity, diabetes mellitus, diabetic complications. Results: In the present review, we describe the potential of root powder of yellow gentian (Gentiana lutea) for the prevention of obesity and diabetes including complications related to this disease. Conclusion: Reasonably effective, low-cost alternatives could fulfill an important role for a large part of the human population and could be of great value for the food market. Even a modest reduction of morbidity and mortality with respect to this disease translates into millions of lives saved. © 2019 Bentham Science Publishers.
T2  - Current Pharmaceutical Design
T1  - Potential of Gentiana lutea for the Treatment of Obesity-associated Diseases
VL  - 25
IS  - 18
SP  - 2071
EP  - 2076
DO  - 10.2174/1381612825666190708215743
ER  - 

@article{
author = "Joksić, Gordana and Filipović Tričković, Jelena G. and Joksić, Ivana",
year = "2019",
abstract = "Background: Obesity, diabetes, and associated diseases are increasing all over the world, and pose a great burden on public health. According to the latest reports, 440 million people are suffering from diabetes. Diabetes is caused by impaired ability to produce or respond to the hormone insulin consequently resulting in hyperglycemia. Methods: Data used for this review was obtained by using PUBMED/MEDLINE (1987-2018). The main data search terms were: Gentiana lutea, Gentiana lutea extract, Gentiana lutea constituents, obesity, diabetes mellitus, diabetic complications. Results: In the present review, we describe the potential of root powder of yellow gentian (Gentiana lutea) for the prevention of obesity and diabetes including complications related to this disease. Conclusion: Reasonably effective, low-cost alternatives could fulfill an important role for a large part of the human population and could be of great value for the food market. Even a modest reduction of morbidity and mortality with respect to this disease translates into millions of lives saved. © 2019 Bentham Science Publishers.",
journal = "Current Pharmaceutical Design",
title = "Potential of Gentiana lutea for the Treatment of Obesity-associated Diseases",
volume = "25",
number = "18",
pages = "2071-2076",
doi = "10.2174/1381612825666190708215743"
}

Joksić, G., Filipović Tričković, J. G.,& Joksić, I.. (2019). Potential of Gentiana lutea for the Treatment of Obesity-associated Diseases. in Current Pharmaceutical Design, 25(18), 2071-2076.
https://doi.org/10.2174/1381612825666190708215743

Joksić G, Filipović Tričković JG, Joksić I. Potential of Gentiana lutea for the Treatment of Obesity-associated Diseases. in Current Pharmaceutical Design. 2019;25(18):2071-2076.
doi:10.2174/1381612825666190708215743 .

Joksić, Gordana, Filipović Tričković, Jelena G., Joksić, Ivana, "Potential of Gentiana lutea for the Treatment of Obesity-associated Diseases" in Current Pharmaceutical Design, 25, no. 18 (2019):2071-2076,
https://doi.org/10.2174/1381612825666190708215743 . .

TY  - JOUR
AU  - Petković, Vladana
AU  - Keta, Otilija D.
AU  - Vidosavljević, Marija Z.
AU  - Incerti, Sebastien
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8414
AB  - Purpose: Investigation of effects on DNA of γ-irradiated human cancer cells pretreated with free radical scavengers is aimed to create reference data which would enable assessment of the relative efficiency of high linear energy transfer (LET) radiations used in hadron therapy, i.e. protons and carbon ions. Materials and methods: MCF-7 breast and HTB177 lung cancer cells are irradiated with γ-rays. To minimize indirect effects of irradiation, dimethyl sulfoxide (DMSO) or glycerol are applied as free radical scavengers. Biological response to irradiation is evaluated through clonogenic cell survival, immunocytochemical and cell cycle analysis, as well as expression of proteins involved in DNA damage response. Results: Examined cell lines reveal similar level of radioresistance. Application of scavengers leads to the rise of cell survival and decreases the number of DNA double strand breaks in irradiated cells. Differences in cell cycle and protein expression between the two cell lines are probably caused by different DNA damage repair mechanisms that are activated. Conclusion: The obtained results show that DMSO and glycerol have good scavenging capacity, and may be used to minimize DNA damage induced by free radicals. Therefore, they will be used as the reference for comparison with high LET irradiations, as well as good experimental data suitable for validation of numerical simulations. © 2019, © 2019 Taylor & Francis Group, LLC.
T2  - International Journal of Radiation Biology
T1  - Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers
VL  - 95
IS  - 3
SP  - 274
EP  - 285
DO  - 10.1080/09553002.2019.1549753
ER  - 

@article{
author = "Petković, Vladana and Keta, Otilija D. and Vidosavljević, Marija Z. and Incerti, Sebastien and Ristić-Fira, Aleksandra and Petrović, Ivan M.",
year = "2019",
abstract = "Purpose: Investigation of effects on DNA of γ-irradiated human cancer cells pretreated with free radical scavengers is aimed to create reference data which would enable assessment of the relative efficiency of high linear energy transfer (LET) radiations used in hadron therapy, i.e. protons and carbon ions. Materials and methods: MCF-7 breast and HTB177 lung cancer cells are irradiated with γ-rays. To minimize indirect effects of irradiation, dimethyl sulfoxide (DMSO) or glycerol are applied as free radical scavengers. Biological response to irradiation is evaluated through clonogenic cell survival, immunocytochemical and cell cycle analysis, as well as expression of proteins involved in DNA damage response. Results: Examined cell lines reveal similar level of radioresistance. Application of scavengers leads to the rise of cell survival and decreases the number of DNA double strand breaks in irradiated cells. Differences in cell cycle and protein expression between the two cell lines are probably caused by different DNA damage repair mechanisms that are activated. Conclusion: The obtained results show that DMSO and glycerol have good scavenging capacity, and may be used to minimize DNA damage induced by free radicals. Therefore, they will be used as the reference for comparison with high LET irradiations, as well as good experimental data suitable for validation of numerical simulations. © 2019, © 2019 Taylor & Francis Group, LLC.",
journal = "International Journal of Radiation Biology",
title = "Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers",
volume = "95",
number = "3",
pages = "274-285",
doi = "10.1080/09553002.2019.1549753"
}

Petković, V., Keta, O. D., Vidosavljević, M. Z., Incerti, S., Ristić-Fira, A.,& Petrović, I. M.. (2019). Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers. in International Journal of Radiation Biology, 95(3), 274-285.
https://doi.org/10.1080/09553002.2019.1549753

Petković V, Keta OD, Vidosavljević MZ, Incerti S, Ristić-Fira A, Petrović IM. Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers. in International Journal of Radiation Biology. 2019;95(3):274-285.
doi:10.1080/09553002.2019.1549753 .

Petković, Vladana, Keta, Otilija D., Vidosavljević, Marija Z., Incerti, Sebastien, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Biological outcomes of γ-radiation induced DNA damages in breast and lung cancer cells pretreated with free radical scavengers" in International Journal of Radiation Biology, 95, no. 3 (2019):274-285,
https://doi.org/10.1080/09553002.2019.1549753 . .

TY  - JOUR
AU  - Nešić, Maja A.
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Stepić, Milutin
AU  - Radoičić, Marija B.
AU  - Popović, Iva A.
AU  - Šaponjić, Zoran
AU  - Petković, Marijana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1747
AB  - We studied the colloidal TiO2 nanoparticles as a carrier for controlled delivery of the ruthenium complex to the melanoma cell line. The system demonstrated slower complex release upon visible and increased release rate upon UV light illumination. Accordingly, the light-dependent cytotoxicity of the system was demonstrated on amelanotic melanoma cancer line. The cell death is enhanced by UV and reduced by red light in the presence of investigated nanocomposite system. Both components of the system may act as photosensitizers, by generating reactive oxygen species, which promote cell death. Thus, the system might act dually, as photodynamic therapeutic agent and as the light tunable system for metallo-drug delivery and it might be of interest for development of new more efficient drug delivery approaches by using a light as external stimulus. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Journal of Photochemistry and Photobiology. A: Chemistry
T1  - Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex
VL  - 347
SP  - 55
EP  - 66
DO  - 10.1016/jjphotochem.2017.06.045
ER  - 

@article{
author = "Nešić, Maja A. and Žakula, Jelena and Korićanac, Lela and Stepić, Milutin and Radoičić, Marija B. and Popović, Iva A. and Šaponjić, Zoran and Petković, Marijana",
year = "2017",
abstract = "We studied the colloidal TiO2 nanoparticles as a carrier for controlled delivery of the ruthenium complex to the melanoma cell line. The system demonstrated slower complex release upon visible and increased release rate upon UV light illumination. Accordingly, the light-dependent cytotoxicity of the system was demonstrated on amelanotic melanoma cancer line. The cell death is enhanced by UV and reduced by red light in the presence of investigated nanocomposite system. Both components of the system may act as photosensitizers, by generating reactive oxygen species, which promote cell death. Thus, the system might act dually, as photodynamic therapeutic agent and as the light tunable system for metallo-drug delivery and it might be of interest for development of new more efficient drug delivery approaches by using a light as external stimulus. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Journal of Photochemistry and Photobiology. A: Chemistry",
title = "Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex",
volume = "347",
pages = "55-66",
doi = "10.1016/jjphotochem.2017.06.045"
}

Nešić, M. A., Žakula, J., Korićanac, L., Stepić, M., Radoičić, M. B., Popović, I. A., Šaponjić, Z.,& Petković, M.. (2017). Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex. in Journal of Photochemistry and Photobiology. A: Chemistry, 347, 55-66.
https://doi.org/10.1016/jjphotochem.2017.06.045

Nešić MA, Žakula J, Korićanac L, Stepić M, Radoičić MB, Popović IA, Šaponjić Z, Petković M. Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex. in Journal of Photochemistry and Photobiology. A: Chemistry. 2017;347:55-66.
doi:10.1016/jjphotochem.2017.06.045 .

Nešić, Maja A., Žakula, Jelena, Korićanac, Lela, Stepić, Milutin, Radoičić, Marija B., Popović, Iva A., Šaponjić, Zoran, Petković, Marijana, "Light controlled metallo-drug delivery system based on the TiO(2-)nanoparticles and Ru-complex" in Journal of Photochemistry and Photobiology. A: Chemistry, 347 (2017):55-66,
https://doi.org/10.1016/jjphotochem.2017.06.045 . .

TY  - JOUR
AU  - Filipović Tričković, Jelena G.
AU  - Mandušić, Vesna
AU  - Joksić, Ivana
AU  - Vujić, Dragana
AU  - Valenta-Šobot, Ana
AU  - Joksić, Gordana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1882
AB  - Fanconi anemia is rare inherited disease characterized by wide spectrum of congenital anomalies, progressive pancytopenia, and predisposition to hematological malignancies and solid tumors. Molecular genetic analysis of mutations in FANC genes is of a great importance for diagnosis confirmation, prenatal and carrier testing, as well as for prediction of chemotherapy outcome and disease complications. In this study we performed screening of frequently affected regions of FANCD2 gene for sequence variants in six unrelated FA-D2 patients in Serbia. This is the first molecular analysis of FANCD2 gene in Serbian FA-D2 patients. A total of 10 sequence variants were detected, one in homozygous, and nine in heterozygous state. Two variants were found within exons, and eight within introns, in deep intronic regions. In-silico analysis showed that among all detected variants one exon variant and three intron variants might have impact on splicing mechanism. Heterozygous variants found in intron 3, c. 206-246delG; exon 26, c. 2396 C GT A and intron 28, c. 2715+573 C GT T were not previously reported. In-silico analysis revealed that among them, two (intron 3, c. 206-246 delG and exon 26, c. 2396 C GT A) could be novel disease-causing mutations. Many variants were found in more than one patient, including those unreported, indicating their possible ethnic association. Great number of variants in some patients suggests their non-random emergence in Fanconi anemia pathway.
T2  - Genetika
T1  - Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants
VL  - 49
IS  - 2
SP  - 559
EP  - 572
DO  - 10.2298/GENSR1702559T
ER  - 

@article{
author = "Filipović Tričković, Jelena G. and Mandušić, Vesna and Joksić, Ivana and Vujić, Dragana and Valenta-Šobot, Ana and Joksić, Gordana",
year = "2017",
abstract = "Fanconi anemia is rare inherited disease characterized by wide spectrum of congenital anomalies, progressive pancytopenia, and predisposition to hematological malignancies and solid tumors. Molecular genetic analysis of mutations in FANC genes is of a great importance for diagnosis confirmation, prenatal and carrier testing, as well as for prediction of chemotherapy outcome and disease complications. In this study we performed screening of frequently affected regions of FANCD2 gene for sequence variants in six unrelated FA-D2 patients in Serbia. This is the first molecular analysis of FANCD2 gene in Serbian FA-D2 patients. A total of 10 sequence variants were detected, one in homozygous, and nine in heterozygous state. Two variants were found within exons, and eight within introns, in deep intronic regions. In-silico analysis showed that among all detected variants one exon variant and three intron variants might have impact on splicing mechanism. Heterozygous variants found in intron 3, c. 206-246delG; exon 26, c. 2396 C GT A and intron 28, c. 2715+573 C GT T were not previously reported. In-silico analysis revealed that among them, two (intron 3, c. 206-246 delG and exon 26, c. 2396 C GT A) could be novel disease-causing mutations. Many variants were found in more than one patient, including those unreported, indicating their possible ethnic association. Great number of variants in some patients suggests their non-random emergence in Fanconi anemia pathway.",
journal = "Genetika",
title = "Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants",
volume = "49",
number = "2",
pages = "559-572",
doi = "10.2298/GENSR1702559T"
}

Filipović Tričković, J. G., Mandušić, V., Joksić, I., Vujić, D., Valenta-Šobot, A.,& Joksić, G.. (2017). Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants. in Genetika, 49(2), 559-572.
https://doi.org/10.2298/GENSR1702559T

Filipović Tričković JG, Mandušić V, Joksić I, Vujić D, Valenta-Šobot A, Joksić G. Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants. in Genetika. 2017;49(2):559-572.
doi:10.2298/GENSR1702559T .

Filipović Tričković, Jelena G., Mandušić, Vesna, Joksić, Ivana, Vujić, Dragana, Valenta-Šobot, Ana, Joksić, Gordana, "Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants" in Genetika, 49, no. 2 (2017):559-572,
https://doi.org/10.2298/GENSR1702559T . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Bulat, Tanja M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1573
AB  - The aim of this study was to investigate effects of irradiations with the therapeutic proton and carbon ion beams in two non-small cell lung cancers, CRL5876 adenocarcinoma and HTB177 large cell lung carcinoma. The DNA damage response dynamics, cell cycle regulation, and cell death pathway activation were followed. Viability of both cell lines was lower after carbon ions compared to the therapeutic proton irradiations. HTB177 cells showed higher recovery than CRL5876 cells seven days following the treatments, but the survival rates of both cell lines were lower after exposure to carbon ions with respect to therapeutic protons. When analyzing cell cycle distribution of both CRL5876 and HTB177 cells, it was noticed that therapeutic protons predominantly induced G1 arrest, while the cells after carbon ions were arrested in G2/M phase. The results illustrated that differences in the levels of phosphorylated H2AX, a double-strand break marker, exist after therapeutic proton and carbon ion irradiations. We also observed dose- and time-dependent increase in the p53 and p21 levels after applied irradiations. Carbon ions caused larger increase in the quantity of p53 and p21 compared to therapeutic protons. These results suggested that various repair mechanisms were induced in the treated cells. Considering the fact that we have not observed any distinct change in the Bax/Bcl-2 ratio following irradiations, it seemed that different types of cell death were involved in the response to the two types of irradiations that were applied.
T2  - Experimental Biology and Medicine
T1  - Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions
VL  - 242
IS  - 10
SP  - 1015
EP  - 1024
DO  - 10.1177/1535370216669611
ER  - 

@article{
author = "Keta, Otilija D. and Todorović, Danijela V. and Bulat, Tanja M. and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2017",
abstract = "The aim of this study was to investigate effects of irradiations with the therapeutic proton and carbon ion beams in two non-small cell lung cancers, CRL5876 adenocarcinoma and HTB177 large cell lung carcinoma. The DNA damage response dynamics, cell cycle regulation, and cell death pathway activation were followed. Viability of both cell lines was lower after carbon ions compared to the therapeutic proton irradiations. HTB177 cells showed higher recovery than CRL5876 cells seven days following the treatments, but the survival rates of both cell lines were lower after exposure to carbon ions with respect to therapeutic protons. When analyzing cell cycle distribution of both CRL5876 and HTB177 cells, it was noticed that therapeutic protons predominantly induced G1 arrest, while the cells after carbon ions were arrested in G2/M phase. The results illustrated that differences in the levels of phosphorylated H2AX, a double-strand break marker, exist after therapeutic proton and carbon ion irradiations. We also observed dose- and time-dependent increase in the p53 and p21 levels after applied irradiations. Carbon ions caused larger increase in the quantity of p53 and p21 compared to therapeutic protons. These results suggested that various repair mechanisms were induced in the treated cells. Considering the fact that we have not observed any distinct change in the Bax/Bcl-2 ratio following irradiations, it seemed that different types of cell death were involved in the response to the two types of irradiations that were applied.",
journal = "Experimental Biology and Medicine",
title = "Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions",
volume = "242",
number = "10",
pages = "1015-1024",
doi = "10.1177/1535370216669611"
}

Keta, O. D., Todorović, D. V., Bulat, T. M., Cirrone, G. A. P., Romano, F., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2017). Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions. in Experimental Biology and Medicine, 242(10), 1015-1024.
https://doi.org/10.1177/1535370216669611

Keta OD, Todorović DV, Bulat TM, Cirrone GAP, Romano F, Cuttone G, Petrović IM, Ristić-Fira A. Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions. in Experimental Biology and Medicine. 2017;242(10):1015-1024.
doi:10.1177/1535370216669611 .

Keta, Otilija D., Todorović, Danijela V., Bulat, Tanja M., Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Comparison of human lung cancer cell radiosensitivity after irradiations with therapeutic protons and carbon ions" in Experimental Biology and Medicine, 242, no. 10 (2017):1015-1024,
https://doi.org/10.1177/1535370216669611 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Mićić, Mileva
AU  - Filipović, Jelena G.
AU  - Drakulić, Dunja R.
AU  - Stanojlović, Miloš R.
AU  - Calija, Bojan
AU  - Valenta-Šobot, Ana
AU  - Demajo, Miroslav
AU  - Nilsson, Robert
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1584
AB  - The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.
T2  - Acta Veterinaria, Beograd
T1  - Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach
VL  - 67
IS  - 1
SP  - 1
EP  - 10
DO  - 10.1515/acve-2017-0001
ER  - 

@article{
author = "Joksić, Gordana and Mićić, Mileva and Filipović, Jelena G. and Drakulić, Dunja R. and Stanojlović, Miloš R. and Calija, Bojan and Valenta-Šobot, Ana and Demajo, Miroslav and Nilsson, Robert",
year = "2017",
abstract = "The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.",
journal = "Acta Veterinaria, Beograd",
title = "Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach",
volume = "67",
number = "1",
pages = "1-10",
doi = "10.1515/acve-2017-0001"
}

Joksić, G., Mićić, M., Filipović, J. G., Drakulić, D. R., Stanojlović, M. R., Calija, B., Valenta-Šobot, A., Demajo, M.,& Nilsson, R.. (2017). Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach. in Acta Veterinaria, Beograd, 67(1), 1-10.
https://doi.org/10.1515/acve-2017-0001

Joksić G, Mićić M, Filipović JG, Drakulić DR, Stanojlović MR, Calija B, Valenta-Šobot A, Demajo M, Nilsson R. Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach. in Acta Veterinaria, Beograd. 2017;67(1):1-10.
doi:10.1515/acve-2017-0001 .

Joksić, Gordana, Mićić, Mileva, Filipović, Jelena G., Drakulić, Dunja R., Stanojlović, Miloš R., Calija, Bojan, Valenta-Šobot, Ana, Demajo, Miroslav, Nilsson, Robert, "Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach" in Acta Veterinaria, Beograd, 67, no. 1 (2017):1-10,
https://doi.org/10.1515/acve-2017-0001 . .

TY  - JOUR
AU  - Bulat, Tanja M.
AU  - Keta, Otilija D.
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/970
AB  - Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (gamma H2AX). Immunofluorescent staining visualizes formation of gamma H2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position and intensity of fluorescent signal in each single cell. In practice there are problems in quantification of gamma H2AX. This paper is based on two issues: the determination of which technique should be applied concerning the radiation dose, and how to analyze fluorescent microscopy images obtained by different microscopes. HTB140 melanoma cells were exposed to gamma-rays, in the dose range from 1 to 16 Gy. Radiation effects on the DNA level were analyzed at different time intervals after irradiation by Western blot analysis and immunofluorescence microscopy. Immunochemically stained cells were visualized with two types of microscopes: AxioVision (Zeiss, Germany) microscope, comprising an ApoTome software, and AxioImagerA1 microscope (Zeiss, Germany). Obtained results show that the level of gamma H2AX is time and dose dependent. Immunofluorescence microscopy provided better detection of DSBs for lower irradiation doses, while Western blot analysis was more reliable for higher irradiation doses. AxioVision microscope containing ApoTome software was more suitable for the detection of gamma H2AX foci.
T2  - Anais de Academia Brasileira de Ciencias
T1  - Radiation dose determines the method for quantification of DNA double strand breaks
VL  - 88
IS  - 1
SP  - 127
EP  - 136
DO  - 10.1590/0001-3765201620140553
ER  - 

@article{
author = "Bulat, Tanja M. and Keta, Otilija D. and Korićanac, Lela and Žakula, Jelena and Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V.",
year = "2016",
abstract = "Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (gamma H2AX). Immunofluorescent staining visualizes formation of gamma H2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position and intensity of fluorescent signal in each single cell. In practice there are problems in quantification of gamma H2AX. This paper is based on two issues: the determination of which technique should be applied concerning the radiation dose, and how to analyze fluorescent microscopy images obtained by different microscopes. HTB140 melanoma cells were exposed to gamma-rays, in the dose range from 1 to 16 Gy. Radiation effects on the DNA level were analyzed at different time intervals after irradiation by Western blot analysis and immunofluorescence microscopy. Immunochemically stained cells were visualized with two types of microscopes: AxioVision (Zeiss, Germany) microscope, comprising an ApoTome software, and AxioImagerA1 microscope (Zeiss, Germany). Obtained results show that the level of gamma H2AX is time and dose dependent. Immunofluorescence microscopy provided better detection of DSBs for lower irradiation doses, while Western blot analysis was more reliable for higher irradiation doses. AxioVision microscope containing ApoTome software was more suitable for the detection of gamma H2AX foci.",
journal = "Anais de Academia Brasileira de Ciencias",
title = "Radiation dose determines the method for quantification of DNA double strand breaks",
volume = "88",
number = "1",
pages = "127-136",
doi = "10.1590/0001-3765201620140553"
}

Bulat, T. M., Keta, O. D., Korićanac, L., Žakula, J., Petrović, I. M., Ristić-Fira, A.,& Todorović, D. V.. (2016). Radiation dose determines the method for quantification of DNA double strand breaks. in Anais de Academia Brasileira de Ciencias, 88(1), 127-136.
https://doi.org/10.1590/0001-3765201620140553

Bulat TM, Keta OD, Korićanac L, Žakula J, Petrović IM, Ristić-Fira A, Todorović DV. Radiation dose determines the method for quantification of DNA double strand breaks. in Anais de Academia Brasileira de Ciencias. 2016;88(1):127-136.
doi:10.1590/0001-3765201620140553 .

Bulat, Tanja M., Keta, Otilija D., Korićanac, Lela, Žakula, Jelena, Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., "Radiation dose determines the method for quantification of DNA double strand breaks" in Anais de Academia Brasileira de Ciencias, 88, no. 1 (2016):127-136,
https://doi.org/10.1590/0001-3765201620140553 . .

TY  - JOUR
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Romano, Francesco
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1290
AB  - Background and objectives: The main goal when treating malignancies with radiation is to deprive tumour cells of their reproductive potential. One approach is to induce tumour cell apoptosis. This study was conducted to evaluate the ability of carbon ions (C-12) to induce apoptosis and cell cycle arrest in human HTB140 melanoma cells. Methods: In this in vitro study, human melanoma HTB140 cells were irradiated with the 62 MeV/n carbon (C-12) ion beam, having two different linear energy transfer (LET) values: 197 and 382 keV/mu m. The dose range was 2 to 16 Gy. Cell viability was estimated by the sulforhodamine B assay seven days after irradiation. The cell cycle and apoptosis were evaluated 48 h after irradiation using flow cytometry. At the same time point, protein and gene expression of apoptotic regulators were estimated using the Western blot and q-PCR methods, respectively. Results: Cell viability experiments indicated strong anti-tumour effects of C-12 ions. The analysis of cell cycle showed that C-12 ions blocked HTB140 cells in G2 phase and induced the dose dependent increase of apoptosis. The maximum value of 21.8 per cent was attained after irradiation with LET of 197 keV/mu m at the dose level of 16 Gy. Pro-apoptotic effects of C-12 ions were confirmed by changes of key apoptotic molecules: the p53, Bax, Bcl-2, poly ADP ribose polymerase (PARP) as well as nuclear factor kappa B (NF kappa B). At the level of protein expression, the results indicated significant increases of p53, NF kappa B and Bax/Bcl-2 ratio and PARP cleavage. The Bax/Bcl-2 mRNA ratio was also increased, while no change was detected in the level of NF kappa B mRNA. Interpretation and conclusions: The present results indicated that anti-tumour effects of C-12 ions in human melanoma HTB140 cells were accomplished through induction of the mitochondrial apoptotic pathway as well as G2 arrest.
T2  - Indian Journal of Medical Research
T1  - Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells
VL  - 143
SP  - 120
EP  - 128
DO  - 10.4103/0971-5916.191811
ER  - 

@article{
author = "Žakula, Jelena and Korićanac, Lela and Keta, Otilija D. and Todorović, Danijela V. and Cirrone, Giuseppe Antonio Pablo and Romano, Francesco and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2016",
abstract = "Background and objectives: The main goal when treating malignancies with radiation is to deprive tumour cells of their reproductive potential. One approach is to induce tumour cell apoptosis. This study was conducted to evaluate the ability of carbon ions (C-12) to induce apoptosis and cell cycle arrest in human HTB140 melanoma cells. Methods: In this in vitro study, human melanoma HTB140 cells were irradiated with the 62 MeV/n carbon (C-12) ion beam, having two different linear energy transfer (LET) values: 197 and 382 keV/mu m. The dose range was 2 to 16 Gy. Cell viability was estimated by the sulforhodamine B assay seven days after irradiation. The cell cycle and apoptosis were evaluated 48 h after irradiation using flow cytometry. At the same time point, protein and gene expression of apoptotic regulators were estimated using the Western blot and q-PCR methods, respectively. Results: Cell viability experiments indicated strong anti-tumour effects of C-12 ions. The analysis of cell cycle showed that C-12 ions blocked HTB140 cells in G2 phase and induced the dose dependent increase of apoptosis. The maximum value of 21.8 per cent was attained after irradiation with LET of 197 keV/mu m at the dose level of 16 Gy. Pro-apoptotic effects of C-12 ions were confirmed by changes of key apoptotic molecules: the p53, Bax, Bcl-2, poly ADP ribose polymerase (PARP) as well as nuclear factor kappa B (NF kappa B). At the level of protein expression, the results indicated significant increases of p53, NF kappa B and Bax/Bcl-2 ratio and PARP cleavage. The Bax/Bcl-2 mRNA ratio was also increased, while no change was detected in the level of NF kappa B mRNA. Interpretation and conclusions: The present results indicated that anti-tumour effects of C-12 ions in human melanoma HTB140 cells were accomplished through induction of the mitochondrial apoptotic pathway as well as G2 arrest.",
journal = "Indian Journal of Medical Research",
title = "Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells",
volume = "143",
pages = "120-128",
doi = "10.4103/0971-5916.191811"
}

Žakula, J., Korićanac, L., Keta, O. D., Todorović, D. V., Cirrone, G. A. P., Romano, F., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2016). Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells. in Indian Journal of Medical Research, 143, 120-128.
https://doi.org/10.4103/0971-5916.191811

Žakula J, Korićanac L, Keta OD, Todorović DV, Cirrone GAP, Romano F, Cuttone G, Petrović IM, Ristić-Fira A. Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells. in Indian Journal of Medical Research. 2016;143:120-128.
doi:10.4103/0971-5916.191811 .

Žakula, Jelena, Korićanac, Lela, Keta, Otilija D., Todorović, Danijela V., Cirrone, Giuseppe Antonio Pablo, Romano, Francesco, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Carbon ions of different linear energy transfer (LET) values induce apoptosis and G2 cell cycle arrest in radio-resistant melanoma cells" in Indian Journal of Medical Research, 143 (2016):120-128,
https://doi.org/10.4103/0971-5916.191811 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Stašić, Jelena
AU  - Filipović, Jelena G.
AU  - Valenta-Šobot, Ana
AU  - Trtica, Milan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/976
AB  - In this work we present biological effects of silver nanoparticles (AgNPs) synthesized by picosecond laser ablation of silver in deionized water. We examined induction of chromosomal aberrations, lymphocyte micronuclei, appearance and recovery of double strand breaks (DSBs) of DNA, cell proliferation potential, concentration of lipid peroxidation products and insulin-like growth factor 1 (ILGF-1). We found that AgNPs sized from 3 nm to 8 nm induce cell cytostasis, which is accompanied with its clastogenic action on DNA, while AgNPs, sized 2 nm behaves contrary stimulating cell proliferation by enhancing ILGF-1 concentration. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
PB  - Elsevier
T2  - Toxicology Letters
T1  - Size of silver nanoparticles determines proliferation ability of human circulating lymphocytes in vitro
VL  - 247
SP  - 29
EP  - 34
DO  - 10.1016/j.toxlet.2016.02.007
ER  - 

@article{
author = "Joksić, Gordana and Stašić, Jelena and Filipović, Jelena G. and Valenta-Šobot, Ana and Trtica, Milan",
year = "2016",
abstract = "In this work we present biological effects of silver nanoparticles (AgNPs) synthesized by picosecond laser ablation of silver in deionized water. We examined induction of chromosomal aberrations, lymphocyte micronuclei, appearance and recovery of double strand breaks (DSBs) of DNA, cell proliferation potential, concentration of lipid peroxidation products and insulin-like growth factor 1 (ILGF-1). We found that AgNPs sized from 3 nm to 8 nm induce cell cytostasis, which is accompanied with its clastogenic action on DNA, while AgNPs, sized 2 nm behaves contrary stimulating cell proliferation by enhancing ILGF-1 concentration. (C) 2016 Elsevier Ireland Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Toxicology Letters",
title = "Size of silver nanoparticles determines proliferation ability of human circulating lymphocytes in vitro",
volume = "247",
pages = "29-34",
doi = "10.1016/j.toxlet.2016.02.007"
}

Joksić, G., Stašić, J., Filipović, J. G., Valenta-Šobot, A.,& Trtica, M.. (2016). Size of silver nanoparticles determines proliferation ability of human circulating lymphocytes in vitro. in Toxicology Letters
Elsevier., 247, 29-34.
https://doi.org/10.1016/j.toxlet.2016.02.007

Joksić G, Stašić J, Filipović JG, Valenta-Šobot A, Trtica M. Size of silver nanoparticles determines proliferation ability of human circulating lymphocytes in vitro. in Toxicology Letters. 2016;247:29-34.
doi:10.1016/j.toxlet.2016.02.007 .

Joksić, Gordana, Stašić, Jelena, Filipović, Jelena G., Valenta-Šobot, Ana, Trtica, Milan, "Size of silver nanoparticles determines proliferation ability of human circulating lymphocytes in vitro" in Toxicology Letters, 247 (2016):29-34,
https://doi.org/10.1016/j.toxlet.2016.02.007 . .

TY  - JOUR
AU  - Filipović, Jelena G.
AU  - Joksić, Gordana
AU  - Vujić, Dragana
AU  - Joksić, Ivana
AU  - Mrasek, Kristin
AU  - Weise, Anja
AU  - Liehr, Thomas
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1239
AB  - Background: Fanconi anemia (FA) is a chromosomal instability syndrome characterized by increased frequency of chromosomal breakages, chromosomal radial figures and accelerated telomere shortening. In this work we performed detailed molecular-cytogenetic characterization of breakpoints in primary lymphocytes of FA-D2 patients in different stages of the disease using fluorescent in situ hybridization. Results: We found that chromosomal breakpoints co-localize on the molecular level with common fragile sites, whereas their distribution pattern depends on the severity of the disease. Telomere quantitative fluorescent in situ hybridization revealed that telomere fusions and radial figures, especially radials which involve telomere sequences are the consequence of critically shortened telomeres that increase with the disease progression and could be considered as a predictive parameter during the course of the disease. Sex chromosomes in FA cells are also involved in radial formation indicating that specific X chromosome regions share homology with autosomes and also could serve as repair templates in resolving DNA damage. Conclusions: FA-D2 chromosomal breakpoints co-localize with common fragile sites, but their distribution pattern depends on the disease stage. Telomere fusions and radials figures which involve telomere sequences are the consequence of shortened telomeres, increase with disease progression and could be of predictive value.
T2  - Molecular Cytogenetics
T1  - First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease
VL  - 9
DO  - 10.1186/s13039-016-0280-6
ER  - 

@article{
author = "Filipović, Jelena G. and Joksić, Gordana and Vujić, Dragana and Joksić, Ivana and Mrasek, Kristin and Weise, Anja and Liehr, Thomas",
year = "2016",
abstract = "Background: Fanconi anemia (FA) is a chromosomal instability syndrome characterized by increased frequency of chromosomal breakages, chromosomal radial figures and accelerated telomere shortening. In this work we performed detailed molecular-cytogenetic characterization of breakpoints in primary lymphocytes of FA-D2 patients in different stages of the disease using fluorescent in situ hybridization. Results: We found that chromosomal breakpoints co-localize on the molecular level with common fragile sites, whereas their distribution pattern depends on the severity of the disease. Telomere quantitative fluorescent in situ hybridization revealed that telomere fusions and radial figures, especially radials which involve telomere sequences are the consequence of critically shortened telomeres that increase with the disease progression and could be considered as a predictive parameter during the course of the disease. Sex chromosomes in FA cells are also involved in radial formation indicating that specific X chromosome regions share homology with autosomes and also could serve as repair templates in resolving DNA damage. Conclusions: FA-D2 chromosomal breakpoints co-localize with common fragile sites, but their distribution pattern depends on the disease stage. Telomere fusions and radials figures which involve telomere sequences are the consequence of shortened telomeres, increase with disease progression and could be of predictive value.",
journal = "Molecular Cytogenetics",
title = "First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease",
volume = "9",
doi = "10.1186/s13039-016-0280-6"
}

Filipović, J. G., Joksić, G., Vujić, D., Joksić, I., Mrasek, K., Weise, A.,& Liehr, T.. (2016). First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease. in Molecular Cytogenetics, 9.
https://doi.org/10.1186/s13039-016-0280-6

Filipović JG, Joksić G, Vujić D, Joksić I, Mrasek K, Weise A, Liehr T. First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease. in Molecular Cytogenetics. 2016;9.
doi:10.1186/s13039-016-0280-6 .

Filipović, Jelena G., Joksić, Gordana, Vujić, Dragana, Joksić, Ivana, Mrasek, Kristin, Weise, Anja, Liehr, Thomas, "First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease" in Molecular Cytogenetics, 9 (2016),
https://doi.org/10.1186/s13039-016-0280-6 . .

TY  - CONF
AU  - Petković, Vladana
AU  - Keta, Otilija D.
AU  - Vojinović, N.
AU  - Incerti, Sebastien
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9207
AB  - Direct  effects  of  radiation  affect  the  DNA  molecule,  causing  DNAdamage and finally  cell death. We examined the role of DMSO and glycerol as free-radical scavengers in HTB177 cells irradiated with gamma rays. Direct effects of   radiation   were   estimated   through   DNA   double   strand   break   (DSB) quantification  and  cell  survival.  Results  of  this  work  revealed  that  chosen concentration   of   DMSO   exhibit   higher   protective   effect   comparing   to glycerol.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
T1  - Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays
SP  - 447
EP  - 450
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9207
ER  - 

@conference{
author = "Petković, Vladana and Keta, Otilija D. and Vojinović, N. and Incerti, Sebastien and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2016",
abstract = "Direct  effects  of  radiation  affect  the  DNA  molecule,  causing  DNAdamage and finally  cell death. We examined the role of DMSO and glycerol as free-radical scavengers in HTB177 cells irradiated with gamma rays. Direct effects of   radiation   were   estimated   through   DNA   double   strand   break   (DSB) quantification  and  cell  survival.  Results  of  this  work  revealed  that  chosen concentration   of   DMSO   exhibit   higher   protective   effect   comparing   to glycerol.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry",
title = "Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays",
pages = "447-450",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9207"
}

Petković, V., Keta, O. D., Vojinović, N., Incerti, S., Petrović, I. M.,& Ristić-Fira, A.. (2016). Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 447-450.
https://hdl.handle.net/21.15107/rcub_vinar_9207

Petković V, Keta OD, Vojinović N, Incerti S, Petrović IM, Ristić-Fira A. Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry. 2016;:447-450.
https://hdl.handle.net/21.15107/rcub_vinar_9207 .

Petković, Vladana, Keta, Otilija D., Vojinović, N., Incerti, Sebastien, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Radio-protective effect of DMSO glycerol in human non-small cell lung cancer irradiated with gamma rays" in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry (2016):447-450,
https://hdl.handle.net/21.15107/rcub_vinar_9207 .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Bulat, Tanja M.
AU  - Golic, Igor
AU  - Incerti, Sebastien
AU  - Korać, Aleksandra
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1044
AB  - In most patients with lung cancer radiation treatment is used either as single agent or in combination with radiosensitizing drugs. However, the mechanisms underlying combined therapy and its impact on different modes of cell death have not yet been fully elucidated. We aimed to examine effects of single and combined treatments with gamma-rays and erlotinib on radioresistant CRL-5876 human lung adenocarcinoma cells with particular emphasis on cell death. CRL-5876 cells were treated with gamma-rays and/or erlotinib and changes in cell cycle, DNA repair dynamics, ultrastructure, nuclear morphology and protein expression were monitored at different time points. To reveal the relationship between types of cell death that arise after these treatments, autophagy was blocked with chloroquine. We found that higher dose of gamma-rays causes G2/M arrest while adding of erlotinib to this treatment decreases the number of cells in S phase. Impact of erlotinib on kinetics of disappearance of irradiation-induced DNA double strand breaks is reflected in the increase of residual gamma-H2AX foci after 24 h. gamma-rays provoke cytoprotective autophagy which precedes development of senescence. Erlotinib predominantly induces apoptosis and enlarges the number of apoptotic cells in the irradiated CRL-5876 cells. Chloroquine improved cytotoxicity induced by radiation and erlotinib, increased apoptosis and decreased senescence in the CRL-5876 cells. The results obtained on CRL-5876 cells indicate significant radiosensitizing effect of erlotinib and suggest that chloroquine in the combination with the above treatments may have an additional antitumor effect in lung adenocarcinoma.
T2  - Cell Biology and Toxicology
T1  - The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib
VL  - 32
IS  - 2
SP  - 83
EP  - 101
DO  - 10.1007/s10565-016-9319-z
ER  - 

@article{
author = "Keta, Otilija D. and Bulat, Tanja M. and Golic, Igor and Incerti, Sebastien and Korać, Aleksandra and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2016",
abstract = "In most patients with lung cancer radiation treatment is used either as single agent or in combination with radiosensitizing drugs. However, the mechanisms underlying combined therapy and its impact on different modes of cell death have not yet been fully elucidated. We aimed to examine effects of single and combined treatments with gamma-rays and erlotinib on radioresistant CRL-5876 human lung adenocarcinoma cells with particular emphasis on cell death. CRL-5876 cells were treated with gamma-rays and/or erlotinib and changes in cell cycle, DNA repair dynamics, ultrastructure, nuclear morphology and protein expression were monitored at different time points. To reveal the relationship between types of cell death that arise after these treatments, autophagy was blocked with chloroquine. We found that higher dose of gamma-rays causes G2/M arrest while adding of erlotinib to this treatment decreases the number of cells in S phase. Impact of erlotinib on kinetics of disappearance of irradiation-induced DNA double strand breaks is reflected in the increase of residual gamma-H2AX foci after 24 h. gamma-rays provoke cytoprotective autophagy which precedes development of senescence. Erlotinib predominantly induces apoptosis and enlarges the number of apoptotic cells in the irradiated CRL-5876 cells. Chloroquine improved cytotoxicity induced by radiation and erlotinib, increased apoptosis and decreased senescence in the CRL-5876 cells. The results obtained on CRL-5876 cells indicate significant radiosensitizing effect of erlotinib and suggest that chloroquine in the combination with the above treatments may have an additional antitumor effect in lung adenocarcinoma.",
journal = "Cell Biology and Toxicology",
title = "The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib",
volume = "32",
number = "2",
pages = "83-101",
doi = "10.1007/s10565-016-9319-z"
}

Keta, O. D., Bulat, T. M., Golic, I., Incerti, S., Korać, A., Petrović, I. M.,& Ristić-Fira, A.. (2016). The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib. in Cell Biology and Toxicology, 32(2), 83-101.
https://doi.org/10.1007/s10565-016-9319-z

Keta OD, Bulat TM, Golic I, Incerti S, Korać A, Petrović IM, Ristić-Fira A. The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib. in Cell Biology and Toxicology. 2016;32(2):83-101.
doi:10.1007/s10565-016-9319-z .

Keta, Otilija D., Bulat, Tanja M., Golic, Igor, Incerti, Sebastien, Korać, Aleksandra, Petrović, Ivan M., Ristić-Fira, Aleksandra, "The impact of autophagy on cell death modalities in CRL-5876 lung adenocarcinoma cells after their exposure to gamma-rays and/or erlotinib" in Cell Biology and Toxicology, 32, no. 2 (2016):83-101,
https://doi.org/10.1007/s10565-016-9319-z . .

TY  - JOUR
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Joksić, Ivana
AU  - Vujić, Dragana
AU  - Valenta-Šobot, Ana
AU  - Filipović, Jelena G.
AU  - Joksić, Gordana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/777
AB  - Fanconi anemia (FA) is a rare genetically heterogeneous disorder associated with bone marrow failure, birth defects and cancer susceptibility. Apart from the disease-causing mutations in FANC genes, the identification of specific DNA variations, such as single nucleotide polymorphisms (SNPs), in other candidate genes may lead to a better clinical description of this condition enabling individualized treatment with improvement of the prognosis. In this study, we have assessed 95 SNPs located in 52 key genes involved in base excision repair (BER), nucleotide excision repair (NER), mismatch repair (MMR), double strand break (DSB) repair and cell cycle control using a DNA repair chip (Asper Biotech, Estonia) which includes most of the common variants for the candidate genes. The SNP genotyping was performed in five FA-D2 patients and in one FA-A patient. The polymorphisms studied were synonymous (n=10), nonsynonymous (missense) (n=52) and in non-coding regions of the genome (introns and 5 and 3 untranslated regions (UTR)) (n=33). Polymorphisms found at the homozygous state are selected for further analysis. Our results have shown a significant inter-individual variability among patients in the type and the frequency of SNPs and also elucidate the need for further studies of polymorphisms located in ATM, APEX APE 1, XRCC1, ERCC2, MSH3, PARP4, NBS1, BARD1, CDKN1B, TP53 and TP53BP1 which may be of great importance for better clinical description of FA. In addition, the present report recommends the use of SNPs as predictive and prognostic genetic markers to individualize therapy of FA patients.
T2  - Genetika
T1  - Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients
VL  - 47
IS  - 2
SP  - 695
EP  - 710
DO  - 10.2298/GENSR1502695P
ER  - 

@article{
author = "Petrović, Sandra and Leskovac, Andreja and Joksić, Ivana and Vujić, Dragana and Valenta-Šobot, Ana and Filipović, Jelena G. and Joksić, Gordana",
year = "2015",
abstract = "Fanconi anemia (FA) is a rare genetically heterogeneous disorder associated with bone marrow failure, birth defects and cancer susceptibility. Apart from the disease-causing mutations in FANC genes, the identification of specific DNA variations, such as single nucleotide polymorphisms (SNPs), in other candidate genes may lead to a better clinical description of this condition enabling individualized treatment with improvement of the prognosis. In this study, we have assessed 95 SNPs located in 52 key genes involved in base excision repair (BER), nucleotide excision repair (NER), mismatch repair (MMR), double strand break (DSB) repair and cell cycle control using a DNA repair chip (Asper Biotech, Estonia) which includes most of the common variants for the candidate genes. The SNP genotyping was performed in five FA-D2 patients and in one FA-A patient. The polymorphisms studied were synonymous (n=10), nonsynonymous (missense) (n=52) and in non-coding regions of the genome (introns and 5 and 3 untranslated regions (UTR)) (n=33). Polymorphisms found at the homozygous state are selected for further analysis. Our results have shown a significant inter-individual variability among patients in the type and the frequency of SNPs and also elucidate the need for further studies of polymorphisms located in ATM, APEX APE 1, XRCC1, ERCC2, MSH3, PARP4, NBS1, BARD1, CDKN1B, TP53 and TP53BP1 which may be of great importance for better clinical description of FA. In addition, the present report recommends the use of SNPs as predictive and prognostic genetic markers to individualize therapy of FA patients.",
journal = "Genetika",
title = "Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients",
volume = "47",
number = "2",
pages = "695-710",
doi = "10.2298/GENSR1502695P"
}

Petrović, S., Leskovac, A., Joksić, I., Vujić, D., Valenta-Šobot, A., Filipović, J. G.,& Joksić, G.. (2015). Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients. in Genetika, 47(2), 695-710.
https://doi.org/10.2298/GENSR1502695P

Petrović S, Leskovac A, Joksić I, Vujić D, Valenta-Šobot A, Filipović JG, Joksić G. Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients. in Genetika. 2015;47(2):695-710.
doi:10.2298/GENSR1502695P .

Petrović, Sandra, Leskovac, Andreja, Joksić, Ivana, Vujić, Dragana, Valenta-Šobot, Ana, Filipović, Jelena G., Joksić, Gordana, "Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients" in Genetika, 47, no. 2 (2015):695-710,
https://doi.org/10.2298/GENSR1502695P . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Todorović, Danijela V.
AU  - Popović, Nataša M.
AU  - Korićanac, Lela
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5447
AB  - Introduction: Proton radiation offers physical advantages over conventional radiation. Radiosensitivity of human 59M ovarian cancer and HTB140 melanoma cells was investigated after exposure to gamma-rays and protons. Material and methods: Irradiations were performed in the middle of a 62 MeV therapeutic proton spread out Bragg peak with doses ranging from 2 to 16 Gy. The mean energy of protons was 34.88+/-2.15 MeV, corresponding to the linear energy transfer of 4.7+/-0.2 keV/mu m. Irradiations with gamma-rays were performed using the same doses. Viability, proliferation and survival were assessed 7 days after both types of irradiation while analyses of cell cycle and apoptosis were performed 48 h after irradiation. Results: Results showed that gamma-rays and protons reduced the number of viable cells for both cell lines, with stronger inactivation achieved after irradiation with protons. Surviving fractions for 59M were 0.91+/-0.01 for gamma-rays and 0.81+/-0.01 for protons, while those for HTB140 cells were 0.93+/-0.01 for gamma-rays and 0.86+/-0.01 for protons. Relative biological effectiveness of protons, being 2.47+/-0.22 for 59M and 2.08+/-0.36 for HTB140, indicated that protons provoked better cell elimination than gamma-rays. After proton irradiation proliferation capacity of the two cell lines was slightly higher as compared to gamma-rays. Proliferation was higher for 59M than for HTB140 cells after both types of irradiation. Induction of apoptosis and G2 arrest detected after proton irradiation were more prominent in 59M cells. Conclusions: The obtained results suggest that protons exert better antitumour effects on ovarian carcinoma and melanoma cells than gamma-rays. The dissimilar response of these cells to radiation is related to their different features.
T2  - Archives of Medical Science
T1  - Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons
VL  - 10
IS  - 3
SP  - 578
EP  - 586
DO  - 10.5114/aoms.2014.43751
ER  - 

@article{
author = "Keta, Otilija D. and Todorović, Danijela V. and Popović, Nataša M. and Korićanac, Lela and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2014",
abstract = "Introduction: Proton radiation offers physical advantages over conventional radiation. Radiosensitivity of human 59M ovarian cancer and HTB140 melanoma cells was investigated after exposure to gamma-rays and protons. Material and methods: Irradiations were performed in the middle of a 62 MeV therapeutic proton spread out Bragg peak with doses ranging from 2 to 16 Gy. The mean energy of protons was 34.88+/-2.15 MeV, corresponding to the linear energy transfer of 4.7+/-0.2 keV/mu m. Irradiations with gamma-rays were performed using the same doses. Viability, proliferation and survival were assessed 7 days after both types of irradiation while analyses of cell cycle and apoptosis were performed 48 h after irradiation. Results: Results showed that gamma-rays and protons reduced the number of viable cells for both cell lines, with stronger inactivation achieved after irradiation with protons. Surviving fractions for 59M were 0.91+/-0.01 for gamma-rays and 0.81+/-0.01 for protons, while those for HTB140 cells were 0.93+/-0.01 for gamma-rays and 0.86+/-0.01 for protons. Relative biological effectiveness of protons, being 2.47+/-0.22 for 59M and 2.08+/-0.36 for HTB140, indicated that protons provoked better cell elimination than gamma-rays. After proton irradiation proliferation capacity of the two cell lines was slightly higher as compared to gamma-rays. Proliferation was higher for 59M than for HTB140 cells after both types of irradiation. Induction of apoptosis and G2 arrest detected after proton irradiation were more prominent in 59M cells. Conclusions: The obtained results suggest that protons exert better antitumour effects on ovarian carcinoma and melanoma cells than gamma-rays. The dissimilar response of these cells to radiation is related to their different features.",
journal = "Archives of Medical Science",
title = "Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons",
volume = "10",
number = "3",
pages = "578-586",
doi = "10.5114/aoms.2014.43751"
}

Keta, O. D., Todorović, D. V., Popović, N. M., Korićanac, L., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2014). Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons. in Archives of Medical Science, 10(3), 578-586.
https://doi.org/10.5114/aoms.2014.43751

Keta OD, Todorović DV, Popović NM, Korićanac L, Cuttone G, Petrović IM, Ristić-Fira A. Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons. in Archives of Medical Science. 2014;10(3):578-586.
doi:10.5114/aoms.2014.43751 .

Keta, Otilija D., Todorović, Danijela V., Popović, Nataša M., Korićanac, Lela, Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Radiosensitivity of human ovarian carcinoma and melanoma cells to gamma-rays and protons" in Archives of Medical Science, 10, no. 3 (2014):578-586,
https://doi.org/10.5114/aoms.2014.43751 . .

TY  - JOUR
AU  - Keta, Otilija D.
AU  - Bulat, Tanja M.
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Cuttone, Giacomo
AU  - Privitera, Giuseppe
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/167
AB  - Molecular targeted cancer therapy is a promising treatment strategy. Considering the central role of the epidermal growth factor receptor in cell proliferation and survival, there are indications that targeted agents like tyrosine kinase inhibitors, i. e., erlotinib, may enhance the antitumor treatment by radiation. The aim of this study is to analyze the inactivation effects of gamma-rays and to test the radiosensitizing potential of erlotinib on human lung adenocarcinoma cells in vitro. Irradiations were performed with doses ranging from 1 Gy to 8 Gy. In order to increase the radiosensitivity of CRL-5876 lung adenocarcinoma cells, the cells were treated with a clinically relevant concentration of 2 mu M erlotinib. The effects of single and combined treatments were monitored using clonogenic survival, cell viability and proliferation assays at different time points. For the detection and visualization of the phosphorylated histone H2AX (gamma-H2AX), an important biological marker of DNA double-strand break formation, fluorescence inununocytochemistry, was performed. The response to the treatment was monitored at four time points: 30 min, 2, 6, and 24 h. Irradiations with gamma-rays resulted in significant cell inactivation regarding all analyzed biological endpoints. Combined treatments revealed consistent cell inactivation. Moreover, compared to gamma-rays alone, elevated levels of gamma-H2AX foci were observed after pretreatment with erlotinib, indicating radiosensitization through impaired DNA repair.
T2  - Nuclear technology and radiation protection
T1  - Radiosensitization of Non-Small Cell Lung Carcinoma By EGFR Inhibition
VL  - 29
IS  - 3
SP  - 233
EP  - 241
DO  - 10.2298/NTRP1403233K
ER  - 

@article{
author = "Keta, Otilija D. and Bulat, Tanja M. and Korićanac, Lela and Žakula, Jelena and Cuttone, Giacomo and Privitera, Giuseppe and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2014",
abstract = "Molecular targeted cancer therapy is a promising treatment strategy. Considering the central role of the epidermal growth factor receptor in cell proliferation and survival, there are indications that targeted agents like tyrosine kinase inhibitors, i. e., erlotinib, may enhance the antitumor treatment by radiation. The aim of this study is to analyze the inactivation effects of gamma-rays and to test the radiosensitizing potential of erlotinib on human lung adenocarcinoma cells in vitro. Irradiations were performed with doses ranging from 1 Gy to 8 Gy. In order to increase the radiosensitivity of CRL-5876 lung adenocarcinoma cells, the cells were treated with a clinically relevant concentration of 2 mu M erlotinib. The effects of single and combined treatments were monitored using clonogenic survival, cell viability and proliferation assays at different time points. For the detection and visualization of the phosphorylated histone H2AX (gamma-H2AX), an important biological marker of DNA double-strand break formation, fluorescence inununocytochemistry, was performed. The response to the treatment was monitored at four time points: 30 min, 2, 6, and 24 h. Irradiations with gamma-rays resulted in significant cell inactivation regarding all analyzed biological endpoints. Combined treatments revealed consistent cell inactivation. Moreover, compared to gamma-rays alone, elevated levels of gamma-H2AX foci were observed after pretreatment with erlotinib, indicating radiosensitization through impaired DNA repair.",
journal = "Nuclear technology and radiation protection",
title = "Radiosensitization of Non-Small Cell Lung Carcinoma By EGFR Inhibition",
volume = "29",
number = "3",
pages = "233-241",
doi = "10.2298/NTRP1403233K"
}

Keta, O. D., Bulat, T. M., Korićanac, L., Žakula, J., Cuttone, G., Privitera, G., Petrović, I. M.,& Ristić-Fira, A.. (2014). Radiosensitization of Non-Small Cell Lung Carcinoma By EGFR Inhibition. in Nuclear technology and radiation protection, 29(3), 233-241.
https://doi.org/10.2298/NTRP1403233K

Keta OD, Bulat TM, Korićanac L, Žakula J, Cuttone G, Privitera G, Petrović IM, Ristić-Fira A. Radiosensitization of Non-Small Cell Lung Carcinoma By EGFR Inhibition. in Nuclear technology and radiation protection. 2014;29(3):233-241.
doi:10.2298/NTRP1403233K .

Keta, Otilija D., Bulat, Tanja M., Korićanac, Lela, Žakula, Jelena, Cuttone, Giacomo, Privitera, Giuseppe, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Radiosensitization of Non-Small Cell Lung Carcinoma By EGFR Inhibition" in Nuclear technology and radiation protection, 29, no. 3 (2014):233-241,
https://doi.org/10.2298/NTRP1403233K . .

TY  - JOUR
AU  - Pagano, Giovanni
AU  - Talamanca, Annarita Aiello
AU  - Castello, Giuseppe
AU  - Cordero, Mario D.
AU  - d'Ischia, Marco
AU  - Gadaleta, Maria Nicola
AU  - Pallardo, Federico V.
AU  - Petrović, Sandra
AU  - Tiano, Luca
AU  - Zatterale, Adriana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/60
AB  - An extensive number of pathologies are associated with mitochondrial dysfunction (MDF) and oxidative stress (OS). Thus, mitochondrial cofactors termed mitochondrial nutrients (MN), such as alpha-lipoic acid (ALA), Coenzyme Q10 (CoQ10), and L-carnitine (CARN) (or its derivatives) have been tested in a number of clinical trials, and this review is focused on the use of MN-based clinical trials. The papers reporting on MN-based clinical trials were retrieved in MedLine up to July 2014, and evaluated for the following endpoints: (a) treated diseases; (b) dosages, number of enrolled patients and duration of treatment; (c) trial success for each MN or MN combinations as reported by authors. The reports satisfying the above endpoints included total numbers of trials and frequencies of randomized, controlled studies, i.e., 81 trials testing ALA, 107 reports testing CoQ10, and 74 reports testing CARN, while only 7 reports were retrieved testing double MN associations, while no report was found testing a triple MN combination. A total of 28 reports tested MN associations with classical antioxidants, such as antioxidant nutrients or drugs. Combinations of MN showed better outcomes than individual MN, suggesting forthcoming clinical studies. The criteria in study design and monitoring MN-based clinical trials are discussed.
T2  - International Journal of Molecular Sciences
T1  - Current Experience in Testing Mitochondrial Nutrients in Disorders Featuring Oxidative Stress and Mitochondrial Dysfunction: Rational Design of Chemoprevention Trials
VL  - 15
IS  - 11
SP  - 20169
EP  - 20208
DO  - 10.3390/ijms151120169
ER  - 

@article{
author = "Pagano, Giovanni and Talamanca, Annarita Aiello and Castello, Giuseppe and Cordero, Mario D. and d'Ischia, Marco and Gadaleta, Maria Nicola and Pallardo, Federico V. and Petrović, Sandra and Tiano, Luca and Zatterale, Adriana",
year = "2014",
abstract = "An extensive number of pathologies are associated with mitochondrial dysfunction (MDF) and oxidative stress (OS). Thus, mitochondrial cofactors termed mitochondrial nutrients (MN), such as alpha-lipoic acid (ALA), Coenzyme Q10 (CoQ10), and L-carnitine (CARN) (or its derivatives) have been tested in a number of clinical trials, and this review is focused on the use of MN-based clinical trials. The papers reporting on MN-based clinical trials were retrieved in MedLine up to July 2014, and evaluated for the following endpoints: (a) treated diseases; (b) dosages, number of enrolled patients and duration of treatment; (c) trial success for each MN or MN combinations as reported by authors. The reports satisfying the above endpoints included total numbers of trials and frequencies of randomized, controlled studies, i.e., 81 trials testing ALA, 107 reports testing CoQ10, and 74 reports testing CARN, while only 7 reports were retrieved testing double MN associations, while no report was found testing a triple MN combination. A total of 28 reports tested MN associations with classical antioxidants, such as antioxidant nutrients or drugs. Combinations of MN showed better outcomes than individual MN, suggesting forthcoming clinical studies. The criteria in study design and monitoring MN-based clinical trials are discussed.",
journal = "International Journal of Molecular Sciences",
title = "Current Experience in Testing Mitochondrial Nutrients in Disorders Featuring Oxidative Stress and Mitochondrial Dysfunction: Rational Design of Chemoprevention Trials",
volume = "15",
number = "11",
pages = "20169-20208",
doi = "10.3390/ijms151120169"
}

Pagano, G., Talamanca, A. A., Castello, G., Cordero, M. D., d'Ischia, M., Gadaleta, M. N., Pallardo, F. V., Petrović, S., Tiano, L.,& Zatterale, A.. (2014). Current Experience in Testing Mitochondrial Nutrients in Disorders Featuring Oxidative Stress and Mitochondrial Dysfunction: Rational Design of Chemoprevention Trials. in International Journal of Molecular Sciences, 15(11), 20169-20208.
https://doi.org/10.3390/ijms151120169

Pagano G, Talamanca AA, Castello G, Cordero MD, d'Ischia M, Gadaleta MN, Pallardo FV, Petrović S, Tiano L, Zatterale A. Current Experience in Testing Mitochondrial Nutrients in Disorders Featuring Oxidative Stress and Mitochondrial Dysfunction: Rational Design of Chemoprevention Trials. in International Journal of Molecular Sciences. 2014;15(11):20169-20208.
doi:10.3390/ijms151120169 .

Pagano, Giovanni, Talamanca, Annarita Aiello, Castello, Giuseppe, Cordero, Mario D., d'Ischia, Marco, Gadaleta, Maria Nicola, Pallardo, Federico V., Petrović, Sandra, Tiano, Luca, Zatterale, Adriana, "Current Experience in Testing Mitochondrial Nutrients in Disorders Featuring Oxidative Stress and Mitochondrial Dysfunction: Rational Design of Chemoprevention Trials" in International Journal of Molecular Sciences, 15, no. 11 (2014):20169-20208,
https://doi.org/10.3390/ijms151120169 . .

TY  - JOUR
AU  - Vujić, Dragana
AU  - Petrović, Sandra
AU  - Lazić, Emilija
AU  - Kuzmanović, Miloš
AU  - Leskovac, Andreja
AU  - Joksić, Ivana
AU  - Mićić, Dragan
AU  - Jovanović, Ankica
AU  - Zečević, Željko
AU  - Guć-Šćekić, Marija
AU  - Ćirković, Sanja
AU  - Joksić, Gordana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5928
AB  - To investigate genetic subtypes of inherited bone marrow failure syndrome Fanconi anemia (FA) in Sebia. FA-D2 subtype was found to be the most frequent genetic subtype among investigated FA patients; specific observations of FA-D2 phenotype are pointed out. Several biological endpoints of FA cells in vitro such as radiation-induced level of lymphocyte micronuclei (radiosensitivity), base line and radiation induced level of the DNA double strand breaks (DSBs), leukocyte apoptosis, and telomere capping function were assessed. The results indicate that all FA-D2 patients display radioresistant in vitro response, which is seen as significantly reduced yield of radiation-induced micronuclei. On the contrary, FA-A patients display radiosensitive in vitro response seen as increased number of radiation-induced micronuclei (MN). A massive elimination of irradiated cells via apoptosis is found in both FA-A and FA-D2 subtypes. In FA-A subtype apoptosis positively relates with the yield of radiation-induced MN, whereas in FA-D2 subtype apoptosis relates with a high percentage of cells carrying dysfunctional telomeres. The present results unequivocally demonstrate that cytokinesis-block micronucleus (CBMN) assay and analyses of telomere capping function can be used to distinguish FA-D2 and FA-A complementation groups. Considering all biological endpoints were analyzed, it can be concluded that all FA patients are radiosensitive, regardless of their complementation group. Thus, using CBMN test and telomere capping function analysis can discriminate FA-A from FA-D2 complementation groups, which could be important for assessment the conditioning regimens prior to bone marrow transplantation.
T2  - Indian Journal of Pediatrics
T1  - Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia
VL  - 81
IS  - 3
SP  - 260
EP  - 265
DO  - 10.1007/s12098-013-1284-4
ER  - 

@article{
author = "Vujić, Dragana and Petrović, Sandra and Lazić, Emilija and Kuzmanović, Miloš and Leskovac, Andreja and Joksić, Ivana and Mićić, Dragan and Jovanović, Ankica and Zečević, Željko and Guć-Šćekić, Marija and Ćirković, Sanja and Joksić, Gordana",
year = "2014",
abstract = "To investigate genetic subtypes of inherited bone marrow failure syndrome Fanconi anemia (FA) in Sebia. FA-D2 subtype was found to be the most frequent genetic subtype among investigated FA patients; specific observations of FA-D2 phenotype are pointed out. Several biological endpoints of FA cells in vitro such as radiation-induced level of lymphocyte micronuclei (radiosensitivity), base line and radiation induced level of the DNA double strand breaks (DSBs), leukocyte apoptosis, and telomere capping function were assessed. The results indicate that all FA-D2 patients display radioresistant in vitro response, which is seen as significantly reduced yield of radiation-induced micronuclei. On the contrary, FA-A patients display radiosensitive in vitro response seen as increased number of radiation-induced micronuclei (MN). A massive elimination of irradiated cells via apoptosis is found in both FA-A and FA-D2 subtypes. In FA-A subtype apoptosis positively relates with the yield of radiation-induced MN, whereas in FA-D2 subtype apoptosis relates with a high percentage of cells carrying dysfunctional telomeres. The present results unequivocally demonstrate that cytokinesis-block micronucleus (CBMN) assay and analyses of telomere capping function can be used to distinguish FA-D2 and FA-A complementation groups. Considering all biological endpoints were analyzed, it can be concluded that all FA patients are radiosensitive, regardless of their complementation group. Thus, using CBMN test and telomere capping function analysis can discriminate FA-A from FA-D2 complementation groups, which could be important for assessment the conditioning regimens prior to bone marrow transplantation.",
journal = "Indian Journal of Pediatrics",
title = "Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia",
volume = "81",
number = "3",
pages = "260-265",
doi = "10.1007/s12098-013-1284-4"
}

Vujić, D., Petrović, S., Lazić, E., Kuzmanović, M., Leskovac, A., Joksić, I., Mićić, D., Jovanović, A., Zečević, Ž., Guć-Šćekić, M., Ćirković, S.,& Joksić, G.. (2014). Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia. in Indian Journal of Pediatrics, 81(3), 260-265.
https://doi.org/10.1007/s12098-013-1284-4

Vujić D, Petrović S, Lazić E, Kuzmanović M, Leskovac A, Joksić I, Mićić D, Jovanović A, Zečević Ž, Guć-Šćekić M, Ćirković S, Joksić G. Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia. in Indian Journal of Pediatrics. 2014;81(3):260-265.
doi:10.1007/s12098-013-1284-4 .

Vujić, Dragana, Petrović, Sandra, Lazić, Emilija, Kuzmanović, Miloš, Leskovac, Andreja, Joksić, Ivana, Mićić, Dragan, Jovanović, Ankica, Zečević, Željko, Guć-Šćekić, Marija, Ćirković, Sanja, Joksić, Gordana, "Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia" in Indian Journal of Pediatrics, 81, no. 3 (2014):260-265,
https://doi.org/10.1007/s12098-013-1284-4 . .

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Guć-Šćekić, Marija
AU  - Vujić, Dragana
AU  - Joksić, Gordana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5986
AB  - Purpose: As the Fanconi anemia (FA) pathway is required for appropriate cell cycle progression through mitosis and the completion of cell division, the aim of the present study was to determine the destiny of FA cells after irradiation in vitro and to elucidate any difference in radiosensitivity between FA and control cells. Materials and methods: Analyses of phosphorylated histone H2AX (gamma-H2AX) foci, micronuclei formation and cell cycle analysis were performed in unirradiated (0 min) and irradiated primary FA fibroblasts and in a control group at different post-irradiation times (30 min, 2 h, 5 h and 24 h). Results: The accumulation of gamma-H2AX foci in irradiated FA fibroblasts was observed. At 24 h post-irradiation, 57% of FA cells were gamma-H2AX foci-positive, significantly higher than in the control (p LT 0.01). The cell cycle analysis has shown the transient G2/M arrest in irradiated FA fibroblasts. The portion of cells in the G2/M phase showed initial increase at 30 min post-irradiation and afterwards decreased over time reaching the pretreatment level 24 h after irradiation. Irradiated FA fibroblasts progressed to abnormal mitosis, as is shown by the production of cells with different nuclear morphologies from binucleated to multinucleated surrounded with micronuclei, and also by a high percentage of foci-positive micronuclei. The majority of radiation-induced micronuclei were gamma-H2AX foci-positive, indicating that radiation-induced micronuclei contain fragments of damaged chromosomes. In contrast, in the control group, most of the micronuclei were classified as gamma-H2AX foci-negative, which indicates that cells with unrepaired damage were blocked before entering mitosis. Conclusion: The results clearly indicate that mitotic catastrophe might be an important cell-death mechanism involved in the response of FA fibroblasts to ionizing radiation.
T2  - International Journal of Radiation Biology
T1  - Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts
VL  - 90
IS  - 5
SP  - 373
EP  - 381
DO  - 10.3109/09553002.2014.892224
ER  - 

@article{
author = "Leskovac, Andreja and Petrović, Sandra and Guć-Šćekić, Marija and Vujić, Dragana and Joksić, Gordana",
year = "2014",
abstract = "Purpose: As the Fanconi anemia (FA) pathway is required for appropriate cell cycle progression through mitosis and the completion of cell division, the aim of the present study was to determine the destiny of FA cells after irradiation in vitro and to elucidate any difference in radiosensitivity between FA and control cells. Materials and methods: Analyses of phosphorylated histone H2AX (gamma-H2AX) foci, micronuclei formation and cell cycle analysis were performed in unirradiated (0 min) and irradiated primary FA fibroblasts and in a control group at different post-irradiation times (30 min, 2 h, 5 h and 24 h). Results: The accumulation of gamma-H2AX foci in irradiated FA fibroblasts was observed. At 24 h post-irradiation, 57% of FA cells were gamma-H2AX foci-positive, significantly higher than in the control (p LT 0.01). The cell cycle analysis has shown the transient G2/M arrest in irradiated FA fibroblasts. The portion of cells in the G2/M phase showed initial increase at 30 min post-irradiation and afterwards decreased over time reaching the pretreatment level 24 h after irradiation. Irradiated FA fibroblasts progressed to abnormal mitosis, as is shown by the production of cells with different nuclear morphologies from binucleated to multinucleated surrounded with micronuclei, and also by a high percentage of foci-positive micronuclei. The majority of radiation-induced micronuclei were gamma-H2AX foci-positive, indicating that radiation-induced micronuclei contain fragments of damaged chromosomes. In contrast, in the control group, most of the micronuclei were classified as gamma-H2AX foci-negative, which indicates that cells with unrepaired damage were blocked before entering mitosis. Conclusion: The results clearly indicate that mitotic catastrophe might be an important cell-death mechanism involved in the response of FA fibroblasts to ionizing radiation.",
journal = "International Journal of Radiation Biology",
title = "Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts",
volume = "90",
number = "5",
pages = "373-381",
doi = "10.3109/09553002.2014.892224"
}

Leskovac, A., Petrović, S., Guć-Šćekić, M., Vujić, D.,& Joksić, G.. (2014). Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts. in International Journal of Radiation Biology, 90(5), 373-381.
https://doi.org/10.3109/09553002.2014.892224

Leskovac A, Petrović S, Guć-Šćekić M, Vujić D, Joksić G. Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts. in International Journal of Radiation Biology. 2014;90(5):373-381.
doi:10.3109/09553002.2014.892224 .

Leskovac, Andreja, Petrović, Sandra, Guć-Šćekić, Marija, Vujić, Dragana, Joksić, Gordana, "Radiation-induced mitotic catastrophe in FANCD2 primary fibroblasts" in International Journal of Radiation Biology, 90, no. 5 (2014):373-381,
https://doi.org/10.3109/09553002.2014.892224 . .

TY  - JOUR
AU  - Lazovic, S.
AU  - Maletić, Dimitrije
AU  - Leskovac, Andreja
AU  - Filipović, Jelena G.
AU  - Puac, N.
AU  - Malović, Gordana N.
AU  - Joksić, Gordana
AU  - Petrović, Z. Lj.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/154
AB  - We use human primary fibroblasts for comparing plasma and gamma rays induced DNA damage. In both cases, DNA strand breaks occur, but of fundamentally different nature. Unlike gamma exposure, contact with plasma predominantly leads to single strand breaks and base-damages, while double strand breaks are mainly consequence of the cell repair mechanisms. Different cell signaling mechanisms are detected confirming this (ataxia telangiectasia mutated - ATM and ataxia telangiectasia and Rad3 related - ATR, respectively). The effective plasma doses can be tuned to match the typical therapeutic doses of 2Gy. Tailoring the effective dose through plasma power and duration of the treatment enables safety precautions mainly by inducing apoptosis and consequently reduced frequency of micronuclei. (C) 2014 AIP Publishing LLC.
T2  - Applied Physics Letters
T1  - Plasma induced DNA damage: Comparison with the effects of ionizing radiation
VL  - 105
IS  - 12
DO  - 10.1063/1.4896626
ER  - 

@article{
author = "Lazovic, S. and Maletić, Dimitrije and Leskovac, Andreja and Filipović, Jelena G. and Puac, N. and Malović, Gordana N. and Joksić, Gordana and Petrović, Z. Lj.",
year = "2014",
abstract = "We use human primary fibroblasts for comparing plasma and gamma rays induced DNA damage. In both cases, DNA strand breaks occur, but of fundamentally different nature. Unlike gamma exposure, contact with plasma predominantly leads to single strand breaks and base-damages, while double strand breaks are mainly consequence of the cell repair mechanisms. Different cell signaling mechanisms are detected confirming this (ataxia telangiectasia mutated - ATM and ataxia telangiectasia and Rad3 related - ATR, respectively). The effective plasma doses can be tuned to match the typical therapeutic doses of 2Gy. Tailoring the effective dose through plasma power and duration of the treatment enables safety precautions mainly by inducing apoptosis and consequently reduced frequency of micronuclei. (C) 2014 AIP Publishing LLC.",
journal = "Applied Physics Letters",
title = "Plasma induced DNA damage: Comparison with the effects of ionizing radiation",
volume = "105",
number = "12",
doi = "10.1063/1.4896626"
}

Lazovic, S., Maletić, D., Leskovac, A., Filipović, J. G., Puac, N., Malović, G. N., Joksić, G.,& Petrović, Z. Lj.. (2014). Plasma induced DNA damage: Comparison with the effects of ionizing radiation. in Applied Physics Letters, 105(12).
https://doi.org/10.1063/1.4896626

Lazovic S, Maletić D, Leskovac A, Filipović JG, Puac N, Malović GN, Joksić G, Petrović ZL. Plasma induced DNA damage: Comparison with the effects of ionizing radiation. in Applied Physics Letters. 2014;105(12).
doi:10.1063/1.4896626 .

Lazovic, S., Maletić, Dimitrije, Leskovac, Andreja, Filipović, Jelena G., Puac, N., Malović, Gordana N., Joksić, Gordana, Petrović, Z. Lj., "Plasma induced DNA damage: Comparison with the effects of ionizing radiation" in Applied Physics Letters, 105, no. 12 (2014),
https://doi.org/10.1063/1.4896626 . .

TY  - JOUR
AU  - Romano, Francesco
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Di Rosa, F.
AU  - Mazzaglia, S. E.
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Varisano, A.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6037
AB  - Fluence, depth absorbed dose and linear energy transfer (LET) distributions of proton and carbon ion beams have been investigated using the Monte Carlo code Geant4 (GEometry ANd Tracking). An open source application was developed with the aim to simulate two typical transport beam lines, one used for ocular therapy and cell irradiations with protons and the other for cell irradiations with carbon ions. This tool allows evaluation of the primary and total dose averaged LET and predict their spatial distribution in voxelized or sliced geometries. In order to reproduce the LET distributions in a realistic way, and also the secondary particles contributions due to nuclear interactions were considered in the computations. Pristine and spread-out Bragg peaks were taken into account both for proton and carbon ion beams, with the maximum energy of 62 MeV/n. Depth dose distributions were compared with experimental data, showing good agreement. Primary and total LET distributions were analysed in order to study the influence of contributions of secondary particles in regions at different depths. A non-negligible influence of high-LET components was found in the entrance channel for proton beams, determining the total dose averaged LET by the factor 3 higher than the primary one. A completely different situation was obtained for carbon ions. In this case, secondary particles mainly contributed in the tail that is after the peak. The results showed how the weight of light and heavy secondary ions can considerably influence the computation of LET depth distributions. This has an important role in the interpretation of results coming from radiobiological experiments and, therefore, in hadron treatment planning procedures.
T2  - Physics in Medicine and Biology
T1  - A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line
VL  - 59
IS  - 12
SP  - 2863
EP  - 2882
DO  - 10.1088/0031-9155/59/12/2863
ER  - 

@article{
author = "Romano, Francesco and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Di Rosa, F. and Mazzaglia, S. E. and Petrović, Ivan M. and Ristić-Fira, Aleksandra and Varisano, A.",
year = "2014",
abstract = "Fluence, depth absorbed dose and linear energy transfer (LET) distributions of proton and carbon ion beams have been investigated using the Monte Carlo code Geant4 (GEometry ANd Tracking). An open source application was developed with the aim to simulate two typical transport beam lines, one used for ocular therapy and cell irradiations with protons and the other for cell irradiations with carbon ions. This tool allows evaluation of the primary and total dose averaged LET and predict their spatial distribution in voxelized or sliced geometries. In order to reproduce the LET distributions in a realistic way, and also the secondary particles contributions due to nuclear interactions were considered in the computations. Pristine and spread-out Bragg peaks were taken into account both for proton and carbon ion beams, with the maximum energy of 62 MeV/n. Depth dose distributions were compared with experimental data, showing good agreement. Primary and total LET distributions were analysed in order to study the influence of contributions of secondary particles in regions at different depths. A non-negligible influence of high-LET components was found in the entrance channel for proton beams, determining the total dose averaged LET by the factor 3 higher than the primary one. A completely different situation was obtained for carbon ions. In this case, secondary particles mainly contributed in the tail that is after the peak. The results showed how the weight of light and heavy secondary ions can considerably influence the computation of LET depth distributions. This has an important role in the interpretation of results coming from radiobiological experiments and, therefore, in hadron treatment planning procedures.",
journal = "Physics in Medicine and Biology",
title = "A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line",
volume = "59",
number = "12",
pages = "2863-2882",
doi = "10.1088/0031-9155/59/12/2863"
}

Romano, F., Cirrone, G. A. P., Cuttone, G., Di Rosa, F., Mazzaglia, S. E., Petrović, I. M., Ristić-Fira, A.,& Varisano, A.. (2014). A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line. in Physics in Medicine and Biology, 59(12), 2863-2882.
https://doi.org/10.1088/0031-9155/59/12/2863

Romano F, Cirrone GAP, Cuttone G, Di Rosa F, Mazzaglia SE, Petrović IM, Ristić-Fira A, Varisano A. A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line. in Physics in Medicine and Biology. 2014;59(12):2863-2882.
doi:10.1088/0031-9155/59/12/2863 .

Romano, Francesco, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Di Rosa, F., Mazzaglia, S. E., Petrović, Ivan M., Ristić-Fira, Aleksandra, Varisano, A., "A Monte Carlo study for the calculation of the average linear energy transfer (LET) distributions for a clinical proton beam line and a radiobiological carbon ion beam line" in Physics in Medicine and Biology, 59, no. 12 (2014):2863-2882,
https://doi.org/10.1088/0031-9155/59/12/2863 . .