TY  - CONF
AU  - Stanković, Miroslav
AU  - Stojanović, Igor
AU  - Zlatanović, Ivana
AU  - Milovanović, Vesna
AU  - Stojanović, Gordana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11147
PB  - RAD Centre, Niš, Serbia
C3  - RAD 2019 : 7th International Conference on Radiation in Various Fields of Research : book of abstracts; June 10-14, 2019; Herceg Novi, Montenegro
T1  - An overview of the effect of Hypogimnia physodes, Hypogimnia tubulosa, Umbilicaria crustulosa and Umbilicaria cylindrica acetone extracts on frequencies and distribution of micronucleus in human lymphocytes
SP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11147
ER  - 

@conference{
author = "Stanković, Miroslav and Stojanović, Igor and Zlatanović, Ivana and Milovanović, Vesna and Stojanović, Gordana",
year = "2019",
publisher = "RAD Centre, Niš, Serbia",
journal = "RAD 2019 : 7th International Conference on Radiation in Various Fields of Research : book of abstracts; June 10-14, 2019; Herceg Novi, Montenegro",
title = "An overview of the effect of Hypogimnia physodes, Hypogimnia tubulosa, Umbilicaria crustulosa and Umbilicaria cylindrica acetone extracts on frequencies and distribution of micronucleus in human lymphocytes",
pages = "140",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11147"
}

Stanković, M., Stojanović, I., Zlatanović, I., Milovanović, V.,& Stojanović, G.. (2019). An overview of the effect of Hypogimnia physodes, Hypogimnia tubulosa, Umbilicaria crustulosa and Umbilicaria cylindrica acetone extracts on frequencies and distribution of micronucleus in human lymphocytes. in RAD 2019 : 7th International Conference on Radiation in Various Fields of Research : book of abstracts; June 10-14, 2019; Herceg Novi, Montenegro
RAD Centre, Niš, Serbia., 140.
https://hdl.handle.net/21.15107/rcub_vinar_11147

Stanković M, Stojanović I, Zlatanović I, Milovanović V, Stojanović G. An overview of the effect of Hypogimnia physodes, Hypogimnia tubulosa, Umbilicaria crustulosa and Umbilicaria cylindrica acetone extracts on frequencies and distribution of micronucleus in human lymphocytes. in RAD 2019 : 7th International Conference on Radiation in Various Fields of Research : book of abstracts; June 10-14, 2019; Herceg Novi, Montenegro. 2019;:140.
https://hdl.handle.net/21.15107/rcub_vinar_11147 .

Stanković, Miroslav, Stojanović, Igor, Zlatanović, Ivana, Milovanović, Vesna, Stojanović, Gordana, "An overview of the effect of Hypogimnia physodes, Hypogimnia tubulosa, Umbilicaria crustulosa and Umbilicaria cylindrica acetone extracts on frequencies and distribution of micronucleus in human lymphocytes" in RAD 2019 : 7th International Conference on Radiation in Various Fields of Research : book of abstracts; June 10-14, 2019; Herceg Novi, Montenegro (2019):140,
https://hdl.handle.net/21.15107/rcub_vinar_11147 .

TY  - JOUR
AU  - Nikolić, Milena D.
AU  - Pavlović, Aleksandra N.
AU  - Mitić, Snežana S.
AU  - Tošić, Snežana B.
AU  - Mitić, Milan N.
AU  - Kaličanin, Biljana M.
AU  - Manojlović, Dragan D.
AU  - Stanković, Dalibor M.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8428
AB  - Background of the study – Among fruits, berry fruits contain a very large complex of chemical compounds that are responsible for their antioxidant properties. As far as we know there is no data available from cyclic voltammetry studies of berry fruits. Objectives – The first objective of the present work was to investigate the electrochemical properties of antioxidants present in four types of berry fruits: strawberries, blackberries, blueberries and red raspberries. The second objective was to establish the correlation between antioxidant capacities obtained from cyclic voltammograms with those obtained using spectrophotometric techniques. Methods – Evaluation of antioxidant activity of 15 berry samples (strawberry, blackberry, blueberry and raspberry) has been carried out using cyclic voltammetry (CV). CVs were taken in the potential range between 0 mV and 800 mV with a scan rate of 100 mV s-1. Results – A pronounced anodic current peak observed between 0.524 V and 0.540 V in all analyzed berry samples can be ascribed to anthocyanins, which are the major contributors to the antioxidant capacity of investigated berries. Results obtained from CVs were compared with those obtained by using DPPH, ABTS, FRAP and RP assays. ACI parameters were determined for each berry sample by assigning all the assays an equal weight. According to obtained results, the highest ACI had blueberry samples, followed by blackberry, raspberry and strawberry samples. Also, high positive correlations were found between antioxidant capacities deduced from CVs with those obtained using FRAP (R2 = 0.8630, p = 0.0006), RP (R2 = 0.7426, p = 0.0004), DPPH (R2 = 0.7680, p = 0.0001) and ABTS (R2 = 0.8710, p = 0.000002). In addition, high positive correlations were also found between total phenols, flavonoids, anthocyanins and CV antioxidant capacity (R2 = 0.8091, p = 0.0005; R2 = 0.8341, p = 0.0002; R2 = 0.7163, p = 0.0007), respectively. Conclusion – Obtained results showed that the cyclic voltammetry is a suitable method for the determination of the reducing capacity based on the electrochemical properties of compounds under the test. © ISHS 2019.
T2  - European Journal of Horticultural Science
T1  - Use of cyclic voltammetry to determine the antioxidant capacity of berry fruits: correlation with spectrophotometric assays
VL  - 84
IS  - 3
SP  - 152
EP  - 160
DO  - 10.17660/eJHS.2019/84.3.5
ER  - 

@article{
author = "Nikolić, Milena D. and Pavlović, Aleksandra N. and Mitić, Snežana S. and Tošić, Snežana B. and Mitić, Milan N. and Kaličanin, Biljana M. and Manojlović, Dragan D. and Stanković, Dalibor M.",
year = "2019",
abstract = "Background of the study – Among fruits, berry fruits contain a very large complex of chemical compounds that are responsible for their antioxidant properties. As far as we know there is no data available from cyclic voltammetry studies of berry fruits. Objectives – The first objective of the present work was to investigate the electrochemical properties of antioxidants present in four types of berry fruits: strawberries, blackberries, blueberries and red raspberries. The second objective was to establish the correlation between antioxidant capacities obtained from cyclic voltammograms with those obtained using spectrophotometric techniques. Methods – Evaluation of antioxidant activity of 15 berry samples (strawberry, blackberry, blueberry and raspberry) has been carried out using cyclic voltammetry (CV). CVs were taken in the potential range between 0 mV and 800 mV with a scan rate of 100 mV s-1. Results – A pronounced anodic current peak observed between 0.524 V and 0.540 V in all analyzed berry samples can be ascribed to anthocyanins, which are the major contributors to the antioxidant capacity of investigated berries. Results obtained from CVs were compared with those obtained by using DPPH, ABTS, FRAP and RP assays. ACI parameters were determined for each berry sample by assigning all the assays an equal weight. According to obtained results, the highest ACI had blueberry samples, followed by blackberry, raspberry and strawberry samples. Also, high positive correlations were found between antioxidant capacities deduced from CVs with those obtained using FRAP (R2 = 0.8630, p = 0.0006), RP (R2 = 0.7426, p = 0.0004), DPPH (R2 = 0.7680, p = 0.0001) and ABTS (R2 = 0.8710, p = 0.000002). In addition, high positive correlations were also found between total phenols, flavonoids, anthocyanins and CV antioxidant capacity (R2 = 0.8091, p = 0.0005; R2 = 0.8341, p = 0.0002; R2 = 0.7163, p = 0.0007), respectively. Conclusion – Obtained results showed that the cyclic voltammetry is a suitable method for the determination of the reducing capacity based on the electrochemical properties of compounds under the test. © ISHS 2019.",
journal = "European Journal of Horticultural Science",
title = "Use of cyclic voltammetry to determine the antioxidant capacity of berry fruits: correlation with spectrophotometric assays",
volume = "84",
number = "3",
pages = "152-160",
doi = "10.17660/eJHS.2019/84.3.5"
}

Nikolić, M. D., Pavlović, A. N., Mitić, S. S., Tošić, S. B., Mitić, M. N., Kaličanin, B. M., Manojlović, D. D.,& Stanković, D. M.. (2019). Use of cyclic voltammetry to determine the antioxidant capacity of berry fruits: correlation with spectrophotometric assays. in European Journal of Horticultural Science, 84(3), 152-160.
https://doi.org/10.17660/eJHS.2019/84.3.5

Nikolić MD, Pavlović AN, Mitić SS, Tošić SB, Mitić MN, Kaličanin BM, Manojlović DD, Stanković DM. Use of cyclic voltammetry to determine the antioxidant capacity of berry fruits: correlation with spectrophotometric assays. in European Journal of Horticultural Science. 2019;84(3):152-160.
doi:10.17660/eJHS.2019/84.3.5 .

Nikolić, Milena D., Pavlović, Aleksandra N., Mitić, Snežana S., Tošić, Snežana B., Mitić, Milan N., Kaličanin, Biljana M., Manojlović, Dragan D., Stanković, Dalibor M., "Use of cyclic voltammetry to determine the antioxidant capacity of berry fruits: correlation with spectrophotometric assays" in European Journal of Horticultural Science, 84, no. 3 (2019):152-160,
https://doi.org/10.17660/eJHS.2019/84.3.5 . .

TY  - JOUR
AU  - Zlatanović, Ivana
AU  - Stanković, Miroslava
AU  - Stankov Jovanović, Vesna
AU  - Mitić, Violeta
AU  - Zrnzevic, Ivana
AU  - Đorđević, Aleksandra
AU  - Stojanović, Gordana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1466
AB  - This paper reports for the first time the effect of an acetone extract of Umbilicaria crustulosa on the micronucleus distribution of human lymphocytes, and on the cholinesterase activity and antioxidant activity by the cupric ion reducing antioxidant capacity (CUPRAC) method. Additionally, the total phenolic compounds (TPC) and the antioxidant properties were estimated via DPPH, ABTS and TRP assays. Moreover, the antibacterial activity against two Gram-positive and three Gram-negative bacteria were determined. Acetone extract of U. crustulosa at concentration of 1 and 2 mu g mL(-1) decreased a frequency of micronuclei (MN) by 10.8 and 16.8 %, respectively, acting more or slightly less than the synthetic protector amifostine (AMF, WR-2721, 11.4 %, at concentration of 1 mu g mL(-1)). The tested extract did not inhibit cholinesterase activity nor did it exhibit activity toward the examined bacteria. The extract reduced the concentration of DPPH and ABTS radicals by 88.7 and 96.2 %, respectively. Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.6197+/-0.0166 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 19.7641+/-1.6546 mu g trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 350.4188 +/-14.587 mu g gallic acid equivalents (GAE) per mg of dry extract. The results of the present study showed that U. crustulosa acetone extract is a promising candidate for in vivo experiments considering its antioxidant activity and protective effect on human lymphocytes.
T2  - Journal of the Serbian Chemical Society
T1  - Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract
VL  - 82
IS  - 2
SP  - 141
EP  - 150
DO  - 10.2298/JSC161019014Z
ER  - 

@article{
author = "Zlatanović, Ivana and Stanković, Miroslava and Stankov Jovanović, Vesna and Mitić, Violeta and Zrnzevic, Ivana and Đorđević, Aleksandra and Stojanović, Gordana",
year = "2017",
abstract = "This paper reports for the first time the effect of an acetone extract of Umbilicaria crustulosa on the micronucleus distribution of human lymphocytes, and on the cholinesterase activity and antioxidant activity by the cupric ion reducing antioxidant capacity (CUPRAC) method. Additionally, the total phenolic compounds (TPC) and the antioxidant properties were estimated via DPPH, ABTS and TRP assays. Moreover, the antibacterial activity against two Gram-positive and three Gram-negative bacteria were determined. Acetone extract of U. crustulosa at concentration of 1 and 2 mu g mL(-1) decreased a frequency of micronuclei (MN) by 10.8 and 16.8 %, respectively, acting more or slightly less than the synthetic protector amifostine (AMF, WR-2721, 11.4 %, at concentration of 1 mu g mL(-1)). The tested extract did not inhibit cholinesterase activity nor did it exhibit activity toward the examined bacteria. The extract reduced the concentration of DPPH and ABTS radicals by 88.7 and 96.2 %, respectively. Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.6197+/-0.0166 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 19.7641+/-1.6546 mu g trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 350.4188 +/-14.587 mu g gallic acid equivalents (GAE) per mg of dry extract. The results of the present study showed that U. crustulosa acetone extract is a promising candidate for in vivo experiments considering its antioxidant activity and protective effect on human lymphocytes.",
journal = "Journal of the Serbian Chemical Society",
title = "Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract",
volume = "82",
number = "2",
pages = "141-150",
doi = "10.2298/JSC161019014Z"
}

Zlatanović, I., Stanković, M., Stankov Jovanović, V., Mitić, V., Zrnzevic, I., Đorđević, A.,& Stojanović, G.. (2017). Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract. in Journal of the Serbian Chemical Society, 82(2), 141-150.
https://doi.org/10.2298/JSC161019014Z

Zlatanović I, Stanković M, Stankov Jovanović V, Mitić V, Zrnzevic I, Đorđević A, Stojanović G. Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract. in Journal of the Serbian Chemical Society. 2017;82(2):141-150.
doi:10.2298/JSC161019014Z .

Zlatanović, Ivana, Stanković, Miroslava, Stankov Jovanović, Vesna, Mitić, Violeta, Zrnzevic, Ivana, Đorđević, Aleksandra, Stojanović, Gordana, "Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract" in Journal of the Serbian Chemical Society, 82, no. 2 (2017):141-150,
https://doi.org/10.2298/JSC161019014Z . .

TY  - JOUR
AU  - Zrnzevic, Ivana
AU  - Stanković, Miroslava
AU  - Stankov Jovanović, Vesna
AU  - Mitić, Violeta
AU  - Đorđević, Aleksandra
AU  - Zlatanović, Ivana
AU  - Stojanović, Gordana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1656
AB  - In the present investigation, effects of Ramalina capitata acetone extract on micronucleus distribution on human lymphocytes, on cholinesterase activity and antioxidant activity (by the CUPRAC method) were examined, for the first time as well as its HPLC profile. Additionally, total phenolic compounds (TPC), antioxidant properties (estimated via DPPH, ABTS and TRP assays) and antibacterial activity were determined. The predominant phenolic compounds in this extract were evernic, everninic and obtusatic acids. Acetone extract of R. capitata at concentration of 2 mu g mL(-1) decreased a frequency of micronuclei (MN) for 14.8 %. The extract reduces the concentration of DPPH and ABTS radicals for 21.2 and 36.1 % (respectively). Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.4624 +/- 0.1064 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 6.1176 +/- 0.2964 mu g Trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 670.6376 +/- 66.554 mu g galic acid equivalents (GAE) per mg of dry extract. Tested extract at concentration of 2 mg mL(-1) exhibited inhibition effect (5.2 %) on pooled human serum cholinesterase. The antimicrobial assay showed that acetone extract had inhibition effect towards Gram-positive strains. The results of manifested antioxidant activity, reducing the number of micronuclei in human lymphocytes, and antibacterial activity recommends R. capitata extract for further in vivo studies.
T2  - EXCLI Journal
T1  - Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity
VL  - 16
SP  - 679
EP  - 687
DO  - 10.17179/excli2017-301
ER  - 

@article{
author = "Zrnzevic, Ivana and Stanković, Miroslava and Stankov Jovanović, Vesna and Mitić, Violeta and Đorđević, Aleksandra and Zlatanović, Ivana and Stojanović, Gordana",
year = "2017",
abstract = "In the present investigation, effects of Ramalina capitata acetone extract on micronucleus distribution on human lymphocytes, on cholinesterase activity and antioxidant activity (by the CUPRAC method) were examined, for the first time as well as its HPLC profile. Additionally, total phenolic compounds (TPC), antioxidant properties (estimated via DPPH, ABTS and TRP assays) and antibacterial activity were determined. The predominant phenolic compounds in this extract were evernic, everninic and obtusatic acids. Acetone extract of R. capitata at concentration of 2 mu g mL(-1) decreased a frequency of micronuclei (MN) for 14.8 %. The extract reduces the concentration of DPPH and ABTS radicals for 21.2 and 36.1 % (respectively). Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.4624 +/- 0.1064 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 6.1176 +/- 0.2964 mu g Trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 670.6376 +/- 66.554 mu g galic acid equivalents (GAE) per mg of dry extract. Tested extract at concentration of 2 mg mL(-1) exhibited inhibition effect (5.2 %) on pooled human serum cholinesterase. The antimicrobial assay showed that acetone extract had inhibition effect towards Gram-positive strains. The results of manifested antioxidant activity, reducing the number of micronuclei in human lymphocytes, and antibacterial activity recommends R. capitata extract for further in vivo studies.",
journal = "EXCLI Journal",
title = "Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity",
volume = "16",
pages = "679-687",
doi = "10.17179/excli2017-301"
}

Zrnzevic, I., Stanković, M., Stankov Jovanović, V., Mitić, V., Đorđević, A., Zlatanović, I.,& Stojanović, G.. (2017). Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity. in EXCLI Journal, 16, 679-687.
https://doi.org/10.17179/excli2017-301

Zrnzevic I, Stanković M, Stankov Jovanović V, Mitić V, Đorđević A, Zlatanović I, Stojanović G. Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity. in EXCLI Journal. 2017;16:679-687.
doi:10.17179/excli2017-301 .

Zrnzevic, Ivana, Stanković, Miroslava, Stankov Jovanović, Vesna, Mitić, Violeta, Đorđević, Aleksandra, Zlatanović, Ivana, Stojanović, Gordana, "Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity" in EXCLI Journal, 16 (2017):679-687,
https://doi.org/10.17179/excli2017-301 . .

TY  - JOUR
AU  - Kostić, Danijela A.
AU  - Stanković, Miroslava
AU  - Milovanović, Vesna M.
AU  - Milovanović, N.
AU  - Stojanović, Gordana S.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8009
AB  - The contents of phenols, flavonoids anthocyans and antioxidant activity of the water (WE), methanol (ME) and methanol-water (MWE) (50/50, v/v) extracts of M. sylvestris fresh and dry petals were determined. Further, for all above mentioned extracts, in vitro effect of the frequency of micronuclei (MN) on peripheral human lymphocytes was evaluated using cytochalasin-B micronucleus (CBMN) assay. Total phenol content (mg of gallic acid equivalent/g of petals) varied from 13.20 (WE) to 29.20 (ME) of fresh petals and for dry petals from 80 (WE) to 142 (ME). The content of flavonoids (mg of catechin equivalent /g of petals) ranged from 47.50 (WE) to 101.20 (ME) of fresh petals and for dry petals from 439 (WE) to 814 (ME). The anthocyans content (mg of cyanidin-3-O-glucoside/g of petals) ranged from 0.40 (WE) to 0.72 (ME) of fresh petals and for dry petals from 3.13 (WE) to 6.16 (ME). All extracts showed high antioxidant activity (more than 67%). ME of fresh petals showed a greater decrease in the frequency of MN (from 23.9 to 29.3%) in all tested concentrations (0.5; 1.0 and 2.0 µg/ml) compared to standard radio of protectant to Amifostine (20.8%). © 2017, Scibulcom Ltd. All rights reserved.
T2  - Oxidation Communications
T1  - Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte
VL  - 40
IS  - 2
SP  - 602
EP  - 612
UR  - https://hdl.handle.net/21.15107/rcub_vinar_8009
ER  - 

@article{
author = "Kostić, Danijela A. and Stanković, Miroslava and Milovanović, Vesna M. and Milovanović, N. and Stojanović, Gordana S.",
year = "2017",
abstract = "The contents of phenols, flavonoids anthocyans and antioxidant activity of the water (WE), methanol (ME) and methanol-water (MWE) (50/50, v/v) extracts of M. sylvestris fresh and dry petals were determined. Further, for all above mentioned extracts, in vitro effect of the frequency of micronuclei (MN) on peripheral human lymphocytes was evaluated using cytochalasin-B micronucleus (CBMN) assay. Total phenol content (mg of gallic acid equivalent/g of petals) varied from 13.20 (WE) to 29.20 (ME) of fresh petals and for dry petals from 80 (WE) to 142 (ME). The content of flavonoids (mg of catechin equivalent /g of petals) ranged from 47.50 (WE) to 101.20 (ME) of fresh petals and for dry petals from 439 (WE) to 814 (ME). The anthocyans content (mg of cyanidin-3-O-glucoside/g of petals) ranged from 0.40 (WE) to 0.72 (ME) of fresh petals and for dry petals from 3.13 (WE) to 6.16 (ME). All extracts showed high antioxidant activity (more than 67%). ME of fresh petals showed a greater decrease in the frequency of MN (from 23.9 to 29.3%) in all tested concentrations (0.5; 1.0 and 2.0 µg/ml) compared to standard radio of protectant to Amifostine (20.8%). © 2017, Scibulcom Ltd. All rights reserved.",
journal = "Oxidation Communications",
title = "Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte",
volume = "40",
number = "2",
pages = "602-612",
url = "https://hdl.handle.net/21.15107/rcub_vinar_8009"
}

Kostić, D. A., Stanković, M., Milovanović, V. M., Milovanović, N.,& Stojanović, G. S.. (2017). Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte. in Oxidation Communications, 40(2), 602-612.
https://hdl.handle.net/21.15107/rcub_vinar_8009

Kostić DA, Stanković M, Milovanović VM, Milovanović N, Stojanović GS. Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte. in Oxidation Communications. 2017;40(2):602-612.
https://hdl.handle.net/21.15107/rcub_vinar_8009 .

Kostić, Danijela A., Stanković, Miroslava, Milovanović, Vesna M., Milovanović, N., Stojanović, Gordana S., "Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte" in Oxidation Communications, 40, no. 2 (2017):602-612,
https://hdl.handle.net/21.15107/rcub_vinar_8009 .

TY  - JOUR
AU  - Stojanović, Gordana
AU  - Stanković, Miroslava
AU  - Stojanovic, Igor Z.
AU  - Palic, Ivan
AU  - Milovanović, Vesna
AU  - Rancic, Sofija
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5969
AB  - Three lichen secondary metabolites atranorin (1), evernic acid (2), and usnic acid (3), were evaluated for their in vitro clastogenic and antiproliferative effects on human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay at concentrations of 2 mu g/mL, 4 mu g/mL and 6 mu g/mL of final culture solution. The frequency of micronucleus (MN) was scored in binucleated cells, and cytokinesis-block proliferation index (CBPI) was calculated. Among the tested compounds, 3 exhibited the most prominent effect decreasing the frequency of MN in the range of 42.5% - 48.9%, that is about double of the positive control amifostin WR-2721 that reduces MN frequency for 22.0%. The effect of evernic acid was approximately equal to action of amifostin (23.2% -32.9%). Atranorin at concentrations of 2 mu g/mL and 4 mu g/mL decreasing the frequency of MN only for 11.1% and 1.8%, while in concentration of 6 mu g/mL increases the frequency of MN for 9.6 %. The comparable CBPI values of the investigated compounds and control suggested that they did not show a statistically significant inhibitory effect on lymphocyte cell proliferation at applied concentrations.
T2  - Natural Product Communications
T1  - Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes
VL  - 9
IS  - 4
SP  - 503
EP  - 504
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5969
ER  - 

@article{
author = "Stojanović, Gordana and Stanković, Miroslava and Stojanovic, Igor Z. and Palic, Ivan and Milovanović, Vesna and Rancic, Sofija",
year = "2014",
abstract = "Three lichen secondary metabolites atranorin (1), evernic acid (2), and usnic acid (3), were evaluated for their in vitro clastogenic and antiproliferative effects on human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay at concentrations of 2 mu g/mL, 4 mu g/mL and 6 mu g/mL of final culture solution. The frequency of micronucleus (MN) was scored in binucleated cells, and cytokinesis-block proliferation index (CBPI) was calculated. Among the tested compounds, 3 exhibited the most prominent effect decreasing the frequency of MN in the range of 42.5% - 48.9%, that is about double of the positive control amifostin WR-2721 that reduces MN frequency for 22.0%. The effect of evernic acid was approximately equal to action of amifostin (23.2% -32.9%). Atranorin at concentrations of 2 mu g/mL and 4 mu g/mL decreasing the frequency of MN only for 11.1% and 1.8%, while in concentration of 6 mu g/mL increases the frequency of MN for 9.6 %. The comparable CBPI values of the investigated compounds and control suggested that they did not show a statistically significant inhibitory effect on lymphocyte cell proliferation at applied concentrations.",
journal = "Natural Product Communications",
title = "Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes",
volume = "9",
number = "4",
pages = "503-504",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5969"
}

Stojanović, G., Stanković, M., Stojanovic, I. Z., Palic, I., Milovanović, V.,& Rancic, S.. (2014). Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes. in Natural Product Communications, 9(4), 503-504.
https://hdl.handle.net/21.15107/rcub_vinar_5969

Stojanović G, Stanković M, Stojanovic IZ, Palic I, Milovanović V, Rancic S. Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes. in Natural Product Communications. 2014;9(4):503-504.
https://hdl.handle.net/21.15107/rcub_vinar_5969 .

Stojanović, Gordana, Stanković, Miroslava, Stojanovic, Igor Z., Palic, Ivan, Milovanović, Vesna, Rancic, Sofija, "Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes" in Natural Product Communications, 9, no. 4 (2014):503-504,
https://hdl.handle.net/21.15107/rcub_vinar_5969 .

TY  - JOUR
AU  - Stojanovic, Igor Z.
AU  - Stanković, Miroslava
AU  - Jovanovic, Olga
AU  - Petrovic, Goran
AU  - Smelcerovic, Andrija
AU  - Stojanović, Gordana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5302
AB  - Three lichen depsidones, physodalic acid (1), physodic acid (2), and 3-hydroxy physodic acid (3), were isolated from Hypogymnia physodes diethyl ether extract using column chromatography, and their structures determined by comparing their UV, H-1 and C-13 NMR spectroscopic and MS data with those given in the literature, as well as with data computed by CHEM draw ultra 11 software. The contents of 1, 2 and 3 were determined in the methanol (ME), acetone (AE), and diethyl ether (EE) extracts using reversed-phase high performance liquid chromatography with photodiode array detection. The extracts, isolated depsidones 1-3 and EE fraction F23 (consisting of 90% 2 and 3, in the ratio 5.5: 1) were evaluated for their in vitro effects on chromosome aberrations in peripheral human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay in doses of 1 mu g/mL and 2 mu g/mL of final culture solution. The frequency of MN was scored in binucleated cells, and nuclear proliferation index was calculated. It was found that 1, 2, 3, F23, and EE at 1.0 mu g/mL exerted a beneficial effect on lymphocyte cells giving a significant decrease of the frequency of MN in comparison with the positive control Amifostin WR-2721. Among the tested depsidones at a concentration of 1 mu g/mL, 3 exhibited the most prominent effect decreasing the frequency of MN by 30.3%, followed by 2 (28.2%) and 1 (22.0%). The extracts were less effective than the isolated depsidones.
T2  - Natural Product Communications
T1  - Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes
VL  - 8
IS  - 1
SP  - 109
EP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5302
ER  - 

@article{
author = "Stojanovic, Igor Z. and Stanković, Miroslava and Jovanovic, Olga and Petrovic, Goran and Smelcerovic, Andrija and Stojanović, Gordana",
year = "2013",
abstract = "Three lichen depsidones, physodalic acid (1), physodic acid (2), and 3-hydroxy physodic acid (3), were isolated from Hypogymnia physodes diethyl ether extract using column chromatography, and their structures determined by comparing their UV, H-1 and C-13 NMR spectroscopic and MS data with those given in the literature, as well as with data computed by CHEM draw ultra 11 software. The contents of 1, 2 and 3 were determined in the methanol (ME), acetone (AE), and diethyl ether (EE) extracts using reversed-phase high performance liquid chromatography with photodiode array detection. The extracts, isolated depsidones 1-3 and EE fraction F23 (consisting of 90% 2 and 3, in the ratio 5.5: 1) were evaluated for their in vitro effects on chromosome aberrations in peripheral human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay in doses of 1 mu g/mL and 2 mu g/mL of final culture solution. The frequency of MN was scored in binucleated cells, and nuclear proliferation index was calculated. It was found that 1, 2, 3, F23, and EE at 1.0 mu g/mL exerted a beneficial effect on lymphocyte cells giving a significant decrease of the frequency of MN in comparison with the positive control Amifostin WR-2721. Among the tested depsidones at a concentration of 1 mu g/mL, 3 exhibited the most prominent effect decreasing the frequency of MN by 30.3%, followed by 2 (28.2%) and 1 (22.0%). The extracts were less effective than the isolated depsidones.",
journal = "Natural Product Communications",
title = "Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes",
volume = "8",
number = "1",
pages = "109-112",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5302"
}

Stojanovic, I. Z., Stanković, M., Jovanovic, O., Petrovic, G., Smelcerovic, A.,& Stojanović, G.. (2013). Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes. in Natural Product Communications, 8(1), 109-112.
https://hdl.handle.net/21.15107/rcub_vinar_5302

Stojanovic IZ, Stanković M, Jovanovic O, Petrovic G, Smelcerovic A, Stojanović G. Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes. in Natural Product Communications. 2013;8(1):109-112.
https://hdl.handle.net/21.15107/rcub_vinar_5302 .

Stojanovic, Igor Z., Stanković, Miroslava, Jovanovic, Olga, Petrovic, Goran, Smelcerovic, Andrija, Stojanović, Gordana, "Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes" in Natural Product Communications, 8, no. 1 (2013):109-112,
https://hdl.handle.net/21.15107/rcub_vinar_5302 .