TY  - JOUR
AU  - Miler, Marko
AU  - Jarić, Ivana
AU  - Živanović, Jasmina
AU  - Ajdžanovic, Vladimir
AU  - Tanić, Nasta
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1567
AB  - Citrus flavanones naringenin (NAR) and hesperetin (HES) are potent antioxidants that may contribute to maintenance of health at old age by improving cardiovascular and metabolic status. However, they may also affect thyroid hormone economy. Keeping in mind impaired thyroid function at older age, in this study we tested wheather NAR or HES administration potentiate this decline. NAR or HES were administrated orally (15 mg/kg) to male 24-month-old Wistar rats during 4 Weeks. Control groups received vehicle, sunflower oil. Qualitative and quantitative immunohistochemical and immunofluorescent expression of specific proteins and stereological analyses of thyroid tissue were performed. Thyroid stimulating hormone (TSH) and total thyroxine (T-4) concentrations were measured in serum. Thyroid parenchyma of both flavanone-treated groups was characterized by lower (p LT 0.05) absolute and relative volume of luminal colloid, accompanied by elevated (p LT 0.05) relative volume of stroma in comparison with the controls. No hypertrophy or absolute thyroid volume change was detected. Intensity of immunopositive signal for thyroglobulin (Tg) and T-4 bound to Tg (T-4-Tg) increased (p LT 0.05) in the colloid of thyroid follicles after both flavanone treatments. Serum TSH increased (p LT 0.05) after NAR, while T-4 remained unchanged after both treatments. In conclusion, NAR elevated serum TSH in old-aged males, thus being more potent than HES in altering pituitary-thyroid axis. However, changes in thyroid structure, namely moderate colloid depletion and higher Tg and T-4-Tg protein expressions after both treatments, indicate preserved capacity of the gland to compensate flavanone interfering, and maintain T-4 production in old-aged males. (C) 2017 Elsevier GmbH. All rights reserved.
T2  - Acta Histochemica
T1  - Citrus flavanones mildly interfere with pituitary -thyroid axis in old-aged male rats
VL  - 119
IS  - 3
SP  - 292
EP  - 301
DO  - 10.1016/j.acthis.2017.02.005
ER  - 

@article{
author = "Miler, Marko and Jarić, Ivana and Živanović, Jasmina and Ajdžanovic, Vladimir and Tanić, Nasta and Milošević, Verica and Šošić-Jurjević, Branka",
year = "2017",
abstract = "Citrus flavanones naringenin (NAR) and hesperetin (HES) are potent antioxidants that may contribute to maintenance of health at old age by improving cardiovascular and metabolic status. However, they may also affect thyroid hormone economy. Keeping in mind impaired thyroid function at older age, in this study we tested wheather NAR or HES administration potentiate this decline. NAR or HES were administrated orally (15 mg/kg) to male 24-month-old Wistar rats during 4 Weeks. Control groups received vehicle, sunflower oil. Qualitative and quantitative immunohistochemical and immunofluorescent expression of specific proteins and stereological analyses of thyroid tissue were performed. Thyroid stimulating hormone (TSH) and total thyroxine (T-4) concentrations were measured in serum. Thyroid parenchyma of both flavanone-treated groups was characterized by lower (p LT 0.05) absolute and relative volume of luminal colloid, accompanied by elevated (p LT 0.05) relative volume of stroma in comparison with the controls. No hypertrophy or absolute thyroid volume change was detected. Intensity of immunopositive signal for thyroglobulin (Tg) and T-4 bound to Tg (T-4-Tg) increased (p LT 0.05) in the colloid of thyroid follicles after both flavanone treatments. Serum TSH increased (p LT 0.05) after NAR, while T-4 remained unchanged after both treatments. In conclusion, NAR elevated serum TSH in old-aged males, thus being more potent than HES in altering pituitary-thyroid axis. However, changes in thyroid structure, namely moderate colloid depletion and higher Tg and T-4-Tg protein expressions after both treatments, indicate preserved capacity of the gland to compensate flavanone interfering, and maintain T-4 production in old-aged males. (C) 2017 Elsevier GmbH. All rights reserved.",
journal = "Acta Histochemica",
title = "Citrus flavanones mildly interfere with pituitary -thyroid axis in old-aged male rats",
volume = "119",
number = "3",
pages = "292-301",
doi = "10.1016/j.acthis.2017.02.005"
}

Miler, M., Jarić, I., Živanović, J., Ajdžanovic, V., Tanić, N., Milošević, V.,& Šošić-Jurjević, B.. (2017). Citrus flavanones mildly interfere with pituitary -thyroid axis in old-aged male rats. in Acta Histochemica, 119(3), 292-301.
https://doi.org/10.1016/j.acthis.2017.02.005

Miler M, Jarić I, Živanović J, Ajdžanovic V, Tanić N, Milošević V, Šošić-Jurjević B. Citrus flavanones mildly interfere with pituitary -thyroid axis in old-aged male rats. in Acta Histochemica. 2017;119(3):292-301.
doi:10.1016/j.acthis.2017.02.005 .

Miler, Marko, Jarić, Ivana, Živanović, Jasmina, Ajdžanovic, Vladimir, Tanić, Nasta, Milošević, Verica, Šošić-Jurjević, Branka, "Citrus flavanones mildly interfere with pituitary -thyroid axis in old-aged male rats" in Acta Histochemica, 119, no. 3 (2017):292-301,
https://doi.org/10.1016/j.acthis.2017.02.005 . .

TY  - JOUR
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka
AU  - Ajdžanovic, Vladimir
AU  - Živanović, Jasmina
AU  - Isenović, Esma R.
AU  - Popovska-Percinic, Florina
AU  - Milošević, Verica
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/535
AB  - Thyroid C-cells produce calcitonin (CT), a hypocalcemic hormone, that acts as an inhibitor of bone resorption. In this study, we investigated the effects of tamoxifen (TAM) as a selective estrogen receptor modulator on thyroid C-cells, trabecular bone and biochemical markers of bone metabolism in an animal model of androgen deficiency, represented by middle-aged orchidectomized (Orx) rats. Fifteen-month-old male Wistar rats were divided into: Orx and sham-operated (SO) groups. Rats from one Orx group were injected subcutaneously with TAM citrate (Orx + TAM; 0.3 mgkg(-1) b.w.), while the rats from SO and a second Orx group received vehicle alone, once a day for 3 weeks. The peroxidase-antiperoxidase method was applied for localization of CT in C-cells. Thyroid C-cells were morphometrically and ultrastructurally analyzed. An ImageJ image-processing program was used to measure bone histomorphometric parameters. Blood serum samples were analyzed for CT, osteocalcin (OC), calcium (Ca2+) and phosphorus (P). Urinary Ca2+ concentrations were measured. TAM treatment significantly increased thyroid C-cell volume (V-c) and serum CT when compared with vehicle-treated Orx rats. Analysis of trabecular microarchitecture of the tibia showed that administration of TAM significantly increased cancellous bone area, trabecular thickness and trabecular number, whereas trabecular separation was significantly decreased compared with vehicle-treated Orx rats. Serum OC and urinary Ca2+ concentrations were significantly lower in comparison with the control Orx group. These results indicate that in our rat model of androgen deficiency, TAM stimulated calcitonin-producing thyroid C-cells and increased trabecular bone mass.
T2  - Journal of Anatomy
T1  - Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency
VL  - 226
IS  - 5
SP  - 489
EP  - 496
DO  - 10.1111/joa.12298
ER  - 

@article{
author = "Filipović, Branko and Šošić-Jurjević, Branka and Ajdžanovic, Vladimir and Živanović, Jasmina and Isenović, Esma R. and Popovska-Percinic, Florina and Milošević, Verica",
year = "2015",
abstract = "Thyroid C-cells produce calcitonin (CT), a hypocalcemic hormone, that acts as an inhibitor of bone resorption. In this study, we investigated the effects of tamoxifen (TAM) as a selective estrogen receptor modulator on thyroid C-cells, trabecular bone and biochemical markers of bone metabolism in an animal model of androgen deficiency, represented by middle-aged orchidectomized (Orx) rats. Fifteen-month-old male Wistar rats were divided into: Orx and sham-operated (SO) groups. Rats from one Orx group were injected subcutaneously with TAM citrate (Orx + TAM; 0.3 mgkg(-1) b.w.), while the rats from SO and a second Orx group received vehicle alone, once a day for 3 weeks. The peroxidase-antiperoxidase method was applied for localization of CT in C-cells. Thyroid C-cells were morphometrically and ultrastructurally analyzed. An ImageJ image-processing program was used to measure bone histomorphometric parameters. Blood serum samples were analyzed for CT, osteocalcin (OC), calcium (Ca2+) and phosphorus (P). Urinary Ca2+ concentrations were measured. TAM treatment significantly increased thyroid C-cell volume (V-c) and serum CT when compared with vehicle-treated Orx rats. Analysis of trabecular microarchitecture of the tibia showed that administration of TAM significantly increased cancellous bone area, trabecular thickness and trabecular number, whereas trabecular separation was significantly decreased compared with vehicle-treated Orx rats. Serum OC and urinary Ca2+ concentrations were significantly lower in comparison with the control Orx group. These results indicate that in our rat model of androgen deficiency, TAM stimulated calcitonin-producing thyroid C-cells and increased trabecular bone mass.",
journal = "Journal of Anatomy",
title = "Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency",
volume = "226",
number = "5",
pages = "489-496",
doi = "10.1111/joa.12298"
}

Filipović, B., Šošić-Jurjević, B., Ajdžanovic, V., Živanović, J., Isenović, E. R., Popovska-Percinic, F.,& Milošević, V.. (2015). Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency. in Journal of Anatomy, 226(5), 489-496.
https://doi.org/10.1111/joa.12298

Filipović B, Šošić-Jurjević B, Ajdžanovic V, Živanović J, Isenović ER, Popovska-Percinic F, Milošević V. Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency. in Journal of Anatomy. 2015;226(5):489-496.
doi:10.1111/joa.12298 .

Filipović, Branko, Šošić-Jurjević, Branka, Ajdžanovic, Vladimir, Živanović, Jasmina, Isenović, Esma R., Popovska-Percinic, Florina, Milošević, Verica, "Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency" in Journal of Anatomy, 226, no. 5 (2015):489-496,
https://doi.org/10.1111/joa.12298 . .

TY  - JOUR
AU  - Ajdžanovic, Vladimir
AU  - Medigovic, Ivana
AU  - Živanović, Jasmina
AU  - Šošić-Jurjević, Branka
AU  - Trifunovic, Svetlana
AU  - Tanić, Nasta
AU  - Milošević, Verica
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/97
AB  - Somatopause, the complex aspect of andropause, is recognizable by reduced growth hormone - GH/insulin-like growth factor 1 axis function in the ageing male. Soy isoflavones (usually genistein and daidzein), which are known for their beneficial effects in the treatment of ageing symptoms, are active in the pituitary, as well. The iromuno-histomorphometric and fluorescent characteristics of pituitary growth hormone secreting cells, in an animal model of andropause, were examined after a treatment with genistein or daidzein. Andropausal Wistar rats were divided into sham operated, orchidectomized and genistein or daidzein treated orchidectomized groups. Genistein or daidzein (30 mg/kg/day) were administered subcutaneously for three weeks, while sham operated and orchidectomized groups received the vehicle alone. Growth hormone secreting cells were identified by the percoxidase-antiperoxidase immuno-histochemical, and inmuno-fluorescent procedure. The main characteristic of growth hormone secreting cells in soy isoflavones treated groups is a weaker immuno-histochemical staining and immuno fluorescent signal compared to sham operated and orchidectomized groups. The growth hormone secreting cell volume in orchidectomized +genistein or +daidzein groups is by 13.8% and 11.9% (p LT 0.05) smaller respectively, in comparison with the orchidectomized group. In orchidectomized +genistein or +daidzein groups, the growth hormone secreting cells relative volume density is by 62.5% and 61.0% lower (p LT 0.05) respectively than for the sham operated group, and decreased by 65.4% and 64.0% (p LT 0.05) respectively, compared to the orchidectomized group. It can be concluded that chronic genistein or daidzein treatment, in an animal model of andropause, attenuates immuno-histomorphometric and fluorescent characteristics of growth hormone secreting cells.
T2  - Acta Veterinaria, Beograd
T1  - Immuno-Histomorphometric and -Fluorescent Characteristics of Gh Cells After Treatment with Genistein Or Daidzein in An Animal Model of Andropause
VL  - 64
IS  - 1
SP  - 93
EP  - 104
DO  - 10.2478/acve-2014-0010
ER  - 

@article{
author = "Ajdžanovic, Vladimir and Medigovic, Ivana and Živanović, Jasmina and Šošić-Jurjević, Branka and Trifunovic, Svetlana and Tanić, Nasta and Milošević, Verica",
year = "2014",
abstract = "Somatopause, the complex aspect of andropause, is recognizable by reduced growth hormone - GH/insulin-like growth factor 1 axis function in the ageing male. Soy isoflavones (usually genistein and daidzein), which are known for their beneficial effects in the treatment of ageing symptoms, are active in the pituitary, as well. The iromuno-histomorphometric and fluorescent characteristics of pituitary growth hormone secreting cells, in an animal model of andropause, were examined after a treatment with genistein or daidzein. Andropausal Wistar rats were divided into sham operated, orchidectomized and genistein or daidzein treated orchidectomized groups. Genistein or daidzein (30 mg/kg/day) were administered subcutaneously for three weeks, while sham operated and orchidectomized groups received the vehicle alone. Growth hormone secreting cells were identified by the percoxidase-antiperoxidase immuno-histochemical, and inmuno-fluorescent procedure. The main characteristic of growth hormone secreting cells in soy isoflavones treated groups is a weaker immuno-histochemical staining and immuno fluorescent signal compared to sham operated and orchidectomized groups. The growth hormone secreting cell volume in orchidectomized +genistein or +daidzein groups is by 13.8% and 11.9% (p LT 0.05) smaller respectively, in comparison with the orchidectomized group. In orchidectomized +genistein or +daidzein groups, the growth hormone secreting cells relative volume density is by 62.5% and 61.0% lower (p LT 0.05) respectively than for the sham operated group, and decreased by 65.4% and 64.0% (p LT 0.05) respectively, compared to the orchidectomized group. It can be concluded that chronic genistein or daidzein treatment, in an animal model of andropause, attenuates immuno-histomorphometric and fluorescent characteristics of growth hormone secreting cells.",
journal = "Acta Veterinaria, Beograd",
title = "Immuno-Histomorphometric and -Fluorescent Characteristics of Gh Cells After Treatment with Genistein Or Daidzein in An Animal Model of Andropause",
volume = "64",
number = "1",
pages = "93-104",
doi = "10.2478/acve-2014-0010"
}

Ajdžanovic, V., Medigovic, I., Živanović, J., Šošić-Jurjević, B., Trifunovic, S., Tanić, N.,& Milošević, V.. (2014). Immuno-Histomorphometric and -Fluorescent Characteristics of Gh Cells After Treatment with Genistein Or Daidzein in An Animal Model of Andropause. in Acta Veterinaria, Beograd, 64(1), 93-104.
https://doi.org/10.2478/acve-2014-0010

Ajdžanovic V, Medigovic I, Živanović J, Šošić-Jurjević B, Trifunovic S, Tanić N, Milošević V. Immuno-Histomorphometric and -Fluorescent Characteristics of Gh Cells After Treatment with Genistein Or Daidzein in An Animal Model of Andropause. in Acta Veterinaria, Beograd. 2014;64(1):93-104.
doi:10.2478/acve-2014-0010 .

Ajdžanovic, Vladimir, Medigovic, Ivana, Živanović, Jasmina, Šošić-Jurjević, Branka, Trifunovic, Svetlana, Tanić, Nasta, Milošević, Verica, "Immuno-Histomorphometric and -Fluorescent Characteristics of Gh Cells After Treatment with Genistein Or Daidzein in An Animal Model of Andropause" in Acta Veterinaria, Beograd, 64, no. 1 (2014):93-104,
https://doi.org/10.2478/acve-2014-0010 . .