TY  - JOUR
AU  - Preljević, Kenan
AU  - Pašić, Ivana
AU  - Vlaović, Milorad
AU  - Matić, Ivana Z.
AU  - Krivokapić, Slađana
AU  - Petrović, Nina
AU  - Stanojković, Tatjana
AU  - Živković, Vladimir
AU  - Perović, Svetlana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12935
AB  - The essential oils of Thymus vulgaris (TVEO) and Thymus serpyllum (TSEO) show different biological activities. The aim of the study was to evaluate the biological activities of TVEO and TSEO from Montenegro. The main components of TVEO were p-cymene (29.52%), thymol (22.8%) and linalool (4.73%) while the main components of TSEO were p-cymene (19.04%), geraniol (11,09%), linalool (9.16%), geranyl acetate (6.49%) and borneol (5.24%). Antioxidant activity determined via DPPH for TVEO was 4.49 and FRAP 1130.27, while for TSEO it was estimated that DPPH was 4.88 μL/mL and FRAP was 701.25 μmol FRAP/L. Both essential oils were active against all tested bacteria, with the highest level of sensitivity of E. coli with MIC of 1.5625 μL/mL. Essential oils showed strong cytotoxic effects on human cancer cell lines, with IC50 values ranging from 0.20 to 0.24 μL/mL for TVEO and from 0.32 to 0.49 μL/mL for TSEO. TVEO caused apoptosis in cervical adenocarcinoma HeLa cells through activation of caspase-3 and caspase-8, while TSEO caused apoptosis through caspase-3. EOs decreased levels of oxidative stress in normal MRC-5 cells. HeLa cells treated with TVEO had reduced MMP2 expression levels, while cells treated with TSEO had lowered MMP2 and MMP9 levels. The treatment of HeLa cells with TVEO increased the levels of miR-16 and miR-34a, indicating potential tumor-suppressive properties. Our findings suggest that Thymus essential oils may be considered as good candidates for further investigation as cancer-chemopreventive and cancer-therapeutic agents.
T2  - Fitoterapia
T1  - Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro
VL  - 174
SP  - 105871
DO  - 10.1016/j.fitote.2024.105871
ER  - 

@article{
author = "Preljević, Kenan and Pašić, Ivana and Vlaović, Milorad and Matić, Ivana Z. and Krivokapić, Slađana and Petrović, Nina and Stanojković, Tatjana and Živković, Vladimir and Perović, Svetlana",
year = "2024",
abstract = "The essential oils of Thymus vulgaris (TVEO) and Thymus serpyllum (TSEO) show different biological activities. The aim of the study was to evaluate the biological activities of TVEO and TSEO from Montenegro. The main components of TVEO were p-cymene (29.52%), thymol (22.8%) and linalool (4.73%) while the main components of TSEO were p-cymene (19.04%), geraniol (11,09%), linalool (9.16%), geranyl acetate (6.49%) and borneol (5.24%). Antioxidant activity determined via DPPH for TVEO was 4.49 and FRAP 1130.27, while for TSEO it was estimated that DPPH was 4.88 μL/mL and FRAP was 701.25 μmol FRAP/L. Both essential oils were active against all tested bacteria, with the highest level of sensitivity of E. coli with MIC of 1.5625 μL/mL. Essential oils showed strong cytotoxic effects on human cancer cell lines, with IC50 values ranging from 0.20 to 0.24 μL/mL for TVEO and from 0.32 to 0.49 μL/mL for TSEO. TVEO caused apoptosis in cervical adenocarcinoma HeLa cells through activation of caspase-3 and caspase-8, while TSEO caused apoptosis through caspase-3. EOs decreased levels of oxidative stress in normal MRC-5 cells. HeLa cells treated with TVEO had reduced MMP2 expression levels, while cells treated with TSEO had lowered MMP2 and MMP9 levels. The treatment of HeLa cells with TVEO increased the levels of miR-16 and miR-34a, indicating potential tumor-suppressive properties. Our findings suggest that Thymus essential oils may be considered as good candidates for further investigation as cancer-chemopreventive and cancer-therapeutic agents.",
journal = "Fitoterapia",
title = "Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro",
volume = "174",
pages = "105871",
doi = "10.1016/j.fitote.2024.105871"
}

Preljević, K., Pašić, I., Vlaović, M., Matić, I. Z., Krivokapić, S., Petrović, N., Stanojković, T., Živković, V.,& Perović, S.. (2024). Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro. in Fitoterapia, 174, 105871.
https://doi.org/10.1016/j.fitote.2024.105871

Preljević K, Pašić I, Vlaović M, Matić IZ, Krivokapić S, Petrović N, Stanojković T, Živković V, Perović S. Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro. in Fitoterapia. 2024;174:105871.
doi:10.1016/j.fitote.2024.105871 .

Preljević, Kenan, Pašić, Ivana, Vlaović, Milorad, Matić, Ivana Z., Krivokapić, Slađana, Petrović, Nina, Stanojković, Tatjana, Živković, Vladimir, Perović, Svetlana, "Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro" in Fitoterapia, 174 (2024):105871,
https://doi.org/10.1016/j.fitote.2024.105871 . .

TY  - JOUR
AU  - Matić, Ivana Z.
AU  - Mraković, Ana
AU  - Rakočević, Zlatko
AU  - Stoiljković, Milovan
AU  - Pavlović, Vladimir B.
AU  - Momić, Tatjana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11544
AB  - Background: Although luteolin has been confirmed as potent anticancer agent, its potential application as therapeutic is limited by its water solubility. To overcome this shortcoming nanoparticle technology approach was applied. Owing to their proven low toxicity and the possibility to be easily functionalized gold nanoparticles (AuNP) were the nanosystem of choice used in this study. Novel luteolin capped gold nanoparticles (AuNPL) were synthesized and their anticancer effect towards human cervical adenocarcinoma HeLa cells was investigated in vitro. Methods: AuNPL were synthesized by reducing chloroauric acid by trisodium citrate with subsequent addition of luteoline during synthesis and their physicochemical characterization was done. AuNPL cytotoxicity against HeLa, human malignant melanoma A375, and normal human keratinocytes HaCaT cells was tested by MTT cell survival assay, and their IC50 values were determined. The capability of AuNPL to induce cell cycle arrest and apoptosis in HeLa cells were demonstrated by flow cytometry. The antioxidant activity of AuNPL was assessed by DPPH⋅ and ABTS⋅þ scavenging assays. Cytoprotective properties of AuNPL towards HaCaT cells were examined by measuring the physiological and H2O2 induced intracellular reactive oxygen species (ROS) levels using flow cytometry. Also, genotoxicity of AuNPL in HaCaT cells was investigated by the single cell alkaline comet assay. Results: Spherical AuNPL, stable in aqueous solution up to six months at 4 ◦C were obtained in the synthesis. The selectivity in the cytotoxic action of AuNPL on HeLa and A375 cancer cells compared with their cytotoxicity on normal keratinocytes HaCaT was observed. AuNPL exerted their cytotoxic activity against HeLa cells through accumulation of the cells in the subG1 phase of the cell cycle, inducing the apoptotic cell death mediated by the activation of caspase-3 − 8, and − 9. AuNPL antioxidative potential was confirmed by DPPH⋅ and ABTS⋅þ scavenging assays. IC50 concentration of AuNPL exerted cytoprotective effect against HaCaT cells by the significant reduction of the physiological intracellular ROS level. Additionally, AuNPL were shown as more cytoprotective towards HaCaT cells then luteolin due to the more successful elimination of H2O2 induced intracellular ROS. Moreover, nontoxic concentrations of AuNPL did not cause considerable DNA damage of HaCaT cells, indicating low genotoxicity of the nanoparticles.
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach
VL  - 80
SP  - 127286
DO  - 10.1016/j.jtemb.2023.127286
ER  - 

@article{
author = "Matić, Ivana Z. and Mraković, Ana and Rakočević, Zlatko and Stoiljković, Milovan and Pavlović, Vladimir B. and Momić, Tatjana",
year = "2023",
abstract = "Background: Although luteolin has been confirmed as potent anticancer agent, its potential application as therapeutic is limited by its water solubility. To overcome this shortcoming nanoparticle technology approach was applied. Owing to their proven low toxicity and the possibility to be easily functionalized gold nanoparticles (AuNP) were the nanosystem of choice used in this study. Novel luteolin capped gold nanoparticles (AuNPL) were synthesized and their anticancer effect towards human cervical adenocarcinoma HeLa cells was investigated in vitro. Methods: AuNPL were synthesized by reducing chloroauric acid by trisodium citrate with subsequent addition of luteoline during synthesis and their physicochemical characterization was done. AuNPL cytotoxicity against HeLa, human malignant melanoma A375, and normal human keratinocytes HaCaT cells was tested by MTT cell survival assay, and their IC50 values were determined. The capability of AuNPL to induce cell cycle arrest and apoptosis in HeLa cells were demonstrated by flow cytometry. The antioxidant activity of AuNPL was assessed by DPPH⋅ and ABTS⋅þ scavenging assays. Cytoprotective properties of AuNPL towards HaCaT cells were examined by measuring the physiological and H2O2 induced intracellular reactive oxygen species (ROS) levels using flow cytometry. Also, genotoxicity of AuNPL in HaCaT cells was investigated by the single cell alkaline comet assay. Results: Spherical AuNPL, stable in aqueous solution up to six months at 4 ◦C were obtained in the synthesis. The selectivity in the cytotoxic action of AuNPL on HeLa and A375 cancer cells compared with their cytotoxicity on normal keratinocytes HaCaT was observed. AuNPL exerted their cytotoxic activity against HeLa cells through accumulation of the cells in the subG1 phase of the cell cycle, inducing the apoptotic cell death mediated by the activation of caspase-3 − 8, and − 9. AuNPL antioxidative potential was confirmed by DPPH⋅ and ABTS⋅þ scavenging assays. IC50 concentration of AuNPL exerted cytoprotective effect against HaCaT cells by the significant reduction of the physiological intracellular ROS level. Additionally, AuNPL were shown as more cytoprotective towards HaCaT cells then luteolin due to the more successful elimination of H2O2 induced intracellular ROS. Moreover, nontoxic concentrations of AuNPL did not cause considerable DNA damage of HaCaT cells, indicating low genotoxicity of the nanoparticles.",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach",
volume = "80",
pages = "127286",
doi = "10.1016/j.jtemb.2023.127286"
}

Matić, I. Z., Mraković, A., Rakočević, Z., Stoiljković, M., Pavlović, V. B.,& Momić, T.. (2023). Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach. in Journal of Trace Elements in Medicine and Biology, 80, 127286.
https://doi.org/10.1016/j.jtemb.2023.127286

Matić IZ, Mraković A, Rakočević Z, Stoiljković M, Pavlović VB, Momić T. Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach. in Journal of Trace Elements in Medicine and Biology. 2023;80:127286.
doi:10.1016/j.jtemb.2023.127286 .

Matić, Ivana Z., Mraković, Ana, Rakočević, Zlatko, Stoiljković, Milovan, Pavlović, Vladimir B., Momić, Tatjana, "Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach" in Journal of Trace Elements in Medicine and Biology, 80 (2023):127286,
https://doi.org/10.1016/j.jtemb.2023.127286 . .

TY  - JOUR
AU  - Milovanović, Jelena
AU  - Vujasinović, Tijana
AU  - Todorović-Raković, Nataša
AU  - Greenman, John
AU  - Hranisavljević, Jelena
AU  - Radulovic, Marko
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11982
AB  - Background: Vascular endothelial growth factor (VEGF) -A and -C act as multifunctional molecules and growth factors, while VE-cadherin (cadherin 5, CDH5) is the endothelial junction protein. Aim: To assess the relationship between intratumoral VEGF -A, -C and CDH5 levels and clinical outcome, in primary, early-stage, breast cancer patients. Patients and methods: The study included 69 node-negative (N0) breast cancer patients, all of whom had not received any prior hormonal or chemotherapeutic systemic therapy that would affect the course of disease. The median follow-up period was 144 months. Intratumoral mRNA levels of VEGF -A, -C and CDH5 were determined by RT-qPCR. Prognostic performance was evaluated by Cox proportional hazards regression, Kaplan-Meier analysis, as well as by the multivariable approach based on the least absolute shrinkage and selection operator (LASSO) logit regression. Classification of patients into the low and high subgroups was performed using the outcome-oriented cut-off point categorization approach. Results: Of the measured mRNAs, only CDH5 mRNA (t = −2.17; p = 0.04) and VEGF-C mRNA (t = −2.41; p = 0.03) showed significant differences between values in patient subgroups with distant metastasis and those without recurrences, respectively. These t-test results were in agreement with the Cox regression by which CDH5 mRNA reached the most pronounced hazard ratio (HR=2.07; p = 0.05), followed by VEGF-C mRNA (HR=1.59; p = 0.005). HR values above 1.0 indicate that high levels of either CDH5 or VEGF-C mRNAs associated with a higher risk of poor clinical outcome. Distant recurrence incidence was 26% for the CDH5high and 3% for the CDH5low subgroup (Kaplan–Meier analysis). Distant recurrence incidence was 23% for the VEGF-Chigh and 0% for VEGF-Clow subgroup. The independent prognostic value of VEGF-C mRNA was confirmed by LASSO regression. Conclusion: Intratumoral VEGF-A levels did not associate with disease outcome in primary, early-stage, breast cancer patients, whilst raised levels of either CDH5 or VEGF-C prognosticated a high risk of distant metastasis.
T2  - Pathology - Research and Practice
T1  - Vascular endothelial growth factor (VEGF) -A, -C and VE-cadherin as potential biomarkers in early breast cancer patients
VL  - 252
SP  - 154923
DO  - 10.1016/j.prp.2023.154923
ER  - 

@article{
author = "Milovanović, Jelena and Vujasinović, Tijana and Todorović-Raković, Nataša and Greenman, John and Hranisavljević, Jelena and Radulovic, Marko",
year = "2023",
abstract = "Background: Vascular endothelial growth factor (VEGF) -A and -C act as multifunctional molecules and growth factors, while VE-cadherin (cadherin 5, CDH5) is the endothelial junction protein. Aim: To assess the relationship between intratumoral VEGF -A, -C and CDH5 levels and clinical outcome, in primary, early-stage, breast cancer patients. Patients and methods: The study included 69 node-negative (N0) breast cancer patients, all of whom had not received any prior hormonal or chemotherapeutic systemic therapy that would affect the course of disease. The median follow-up period was 144 months. Intratumoral mRNA levels of VEGF -A, -C and CDH5 were determined by RT-qPCR. Prognostic performance was evaluated by Cox proportional hazards regression, Kaplan-Meier analysis, as well as by the multivariable approach based on the least absolute shrinkage and selection operator (LASSO) logit regression. Classification of patients into the low and high subgroups was performed using the outcome-oriented cut-off point categorization approach. Results: Of the measured mRNAs, only CDH5 mRNA (t = −2.17; p = 0.04) and VEGF-C mRNA (t = −2.41; p = 0.03) showed significant differences between values in patient subgroups with distant metastasis and those without recurrences, respectively. These t-test results were in agreement with the Cox regression by which CDH5 mRNA reached the most pronounced hazard ratio (HR=2.07; p = 0.05), followed by VEGF-C mRNA (HR=1.59; p = 0.005). HR values above 1.0 indicate that high levels of either CDH5 or VEGF-C mRNAs associated with a higher risk of poor clinical outcome. Distant recurrence incidence was 26% for the CDH5high and 3% for the CDH5low subgroup (Kaplan–Meier analysis). Distant recurrence incidence was 23% for the VEGF-Chigh and 0% for VEGF-Clow subgroup. The independent prognostic value of VEGF-C mRNA was confirmed by LASSO regression. Conclusion: Intratumoral VEGF-A levels did not associate with disease outcome in primary, early-stage, breast cancer patients, whilst raised levels of either CDH5 or VEGF-C prognosticated a high risk of distant metastasis.",
journal = "Pathology - Research and Practice",
title = "Vascular endothelial growth factor (VEGF) -A, -C and VE-cadherin as potential biomarkers in early breast cancer patients",
volume = "252",
pages = "154923",
doi = "10.1016/j.prp.2023.154923"
}

Milovanović, J., Vujasinović, T., Todorović-Raković, N., Greenman, J., Hranisavljević, J.,& Radulovic, M.. (2023). Vascular endothelial growth factor (VEGF) -A, -C and VE-cadherin as potential biomarkers in early breast cancer patients. in Pathology - Research and Practice, 252, 154923.
https://doi.org/10.1016/j.prp.2023.154923

Milovanović J, Vujasinović T, Todorović-Raković N, Greenman J, Hranisavljević J, Radulovic M. Vascular endothelial growth factor (VEGF) -A, -C and VE-cadherin as potential biomarkers in early breast cancer patients. in Pathology - Research and Practice. 2023;252:154923.
doi:10.1016/j.prp.2023.154923 .

Milovanović, Jelena, Vujasinović, Tijana, Todorović-Raković, Nataša, Greenman, John, Hranisavljević, Jelena, Radulovic, Marko, "Vascular endothelial growth factor (VEGF) -A, -C and VE-cadherin as potential biomarkers in early breast cancer patients" in Pathology - Research and Practice, 252 (2023):154923,
https://doi.org/10.1016/j.prp.2023.154923 . .

TY  - JOUR
AU  - Ergün, Sercan
AU  - Sankaranarayanan, Ramamoorthy
AU  - Petrović, Nina
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11568
AB  - COVID-19 is a viral respiratory infection induced by the newly discovered coronavirus SARS-CoV-2. miRNA is an example of a strong and direct regulator of a gene’s transcriptional activity. The interaction between miRNAs and their target molecules is responsible for homeostasis. Virus-derived and host-derived miRNAs are involved in the activity of hiding from immune system cells, inducing the inflammatory reaction through interplay with associated genes, during SARS-COV-2 infection. Interest in miRNAs has raised the comprehension of the machinery and pathophysiology of SARS-COV-2 infection. In this review, the effects and biological roles of miRNAs on SARS-CoV-2 pathogenicity and life cycle are described. The therapeutic potential of miRNAs against SARS-CoV-2 infection are also mentioned.
T2  - Epigenomics
T1  - Clinically informative microRNAs for SARS-CoV-2 infection
VL  - 15
IS  - 13
SP  - 705
EP  - 716
DO  - 10.2217/epi-2023-0179
ER  - 

@article{
author = "Ergün, Sercan and Sankaranarayanan, Ramamoorthy and Petrović, Nina",
year = "2023",
abstract = "COVID-19 is a viral respiratory infection induced by the newly discovered coronavirus SARS-CoV-2. miRNA is an example of a strong and direct regulator of a gene’s transcriptional activity. The interaction between miRNAs and their target molecules is responsible for homeostasis. Virus-derived and host-derived miRNAs are involved in the activity of hiding from immune system cells, inducing the inflammatory reaction through interplay with associated genes, during SARS-COV-2 infection. Interest in miRNAs has raised the comprehension of the machinery and pathophysiology of SARS-COV-2 infection. In this review, the effects and biological roles of miRNAs on SARS-CoV-2 pathogenicity and life cycle are described. The therapeutic potential of miRNAs against SARS-CoV-2 infection are also mentioned.",
journal = "Epigenomics",
title = "Clinically informative microRNAs for SARS-CoV-2 infection",
volume = "15",
number = "13",
pages = "705-716",
doi = "10.2217/epi-2023-0179"
}

Ergün, S., Sankaranarayanan, R.,& Petrović, N.. (2023). Clinically informative microRNAs for SARS-CoV-2 infection. in Epigenomics, 15(13), 705-716.
https://doi.org/10.2217/epi-2023-0179

Ergün S, Sankaranarayanan R, Petrović N. Clinically informative microRNAs for SARS-CoV-2 infection. in Epigenomics. 2023;15(13):705-716.
doi:10.2217/epi-2023-0179 .

Ergün, Sercan, Sankaranarayanan, Ramamoorthy, Petrović, Nina, "Clinically informative microRNAs for SARS-CoV-2 infection" in Epigenomics, 15, no. 13 (2023):705-716,
https://doi.org/10.2217/epi-2023-0179 . .

TY  - JOUR
AU  - Marković, Maja D.
AU  - Tadić, Julijana D.
AU  - Savić, Sanja I.
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana P.
AU  - Mijin, Dušan Ž.
AU  - Panić, Vesna V.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10262
AB  - Researchers are faced with everyday demands for safer and more efficient therapy for many diseases, especially serious one such as various types of cancer. Numerous anticancer drugs are poorly-water soluble and therefore their encapsulation and controlled release remain quite challenge. In present study, we deepened our research of hydrophilic carrier based on poly(methacrylic acid) and casein (PMAC) by investigating its potential for encapsulation and controlled release of novel poorly water-soluble dihydropyrimidion-azo-pyridon compound (DHPMP). DHPMP is a dye that has been proven to show cytotoxic activity against chronic myeloid leukemia K562 cells. By encapsulating DHPMP into the carrier and delivering it into the intestines, DHPMP absorption could be the fastest and the number of therapeutic doses and side effects can be reduced. Carriers based on PMAC and DHPMP (PMAC-DHPMP) were synthetized and characterized by FTIR, SEM and single compression tests. The swelling behavior of PMAC-DHPMP carriers and cumulative DHPMP release were investigated depending on the amount of crosslinker and encapsulated DHPMP in two media which were simulating pH environments in human stomach and intestines. The prolonged and controlled release of DHPMP was achieved. In vitro cytotoxic activity of PMAC-DHPMP carriers against K562 cells and the cell cycle analysis showed great potential of the carriers for application in leukemia treatment.
T2  - Journal of Biomedical Materials Research - Part A
T1  - Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment
DO  - 10.1002/jbm.a.37396
ER  - 

@article{
author = "Marković, Maja D. and Tadić, Julijana D. and Savić, Sanja I. and Matić, Ivana Z. and Stanojković, Tatjana P. and Mijin, Dušan Ž. and Panić, Vesna V.",
year = "2022",
abstract = "Researchers are faced with everyday demands for safer and more efficient therapy for many diseases, especially serious one such as various types of cancer. Numerous anticancer drugs are poorly-water soluble and therefore their encapsulation and controlled release remain quite challenge. In present study, we deepened our research of hydrophilic carrier based on poly(methacrylic acid) and casein (PMAC) by investigating its potential for encapsulation and controlled release of novel poorly water-soluble dihydropyrimidion-azo-pyridon compound (DHPMP). DHPMP is a dye that has been proven to show cytotoxic activity against chronic myeloid leukemia K562 cells. By encapsulating DHPMP into the carrier and delivering it into the intestines, DHPMP absorption could be the fastest and the number of therapeutic doses and side effects can be reduced. Carriers based on PMAC and DHPMP (PMAC-DHPMP) were synthetized and characterized by FTIR, SEM and single compression tests. The swelling behavior of PMAC-DHPMP carriers and cumulative DHPMP release were investigated depending on the amount of crosslinker and encapsulated DHPMP in two media which were simulating pH environments in human stomach and intestines. The prolonged and controlled release of DHPMP was achieved. In vitro cytotoxic activity of PMAC-DHPMP carriers against K562 cells and the cell cycle analysis showed great potential of the carriers for application in leukemia treatment.",
journal = "Journal of Biomedical Materials Research - Part A",
title = "Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment",
doi = "10.1002/jbm.a.37396"
}

Marković, M. D., Tadić, J. D., Savić, S. I., Matić, I. Z., Stanojković, T. P., Mijin, D. Ž.,& Panić, V. V.. (2022). Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment. in Journal of Biomedical Materials Research - Part A.
https://doi.org/10.1002/jbm.a.37396

Marković MD, Tadić JD, Savić SI, Matić IZ, Stanojković TP, Mijin DŽ, Panić VV. Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment. in Journal of Biomedical Materials Research - Part A. 2022;.
doi:10.1002/jbm.a.37396 .

Marković, Maja D., Tadić, Julijana D., Savić, Sanja I., Matić, Ivana Z., Stanojković, Tatjana P., Mijin, Dušan Ž., Panić, Vesna V., "Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment" in Journal of Biomedical Materials Research - Part A (2022),
https://doi.org/10.1002/jbm.a.37396 . .

TY  - JOUR
AU  - Joksimović, Nenad
AU  - Petronijević, Jelena
AU  - Radisavljević, Snežana
AU  - Petrović, Biljana
AU  - Mihajlović, Kristina
AU  - Janković, Nenad
AU  - Milović, Emilija
AU  - Milivojević, Dušan
AU  - Ilić, Bojana
AU  - Đurić, Ana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10518
AB  - Considering the urgency of finding a cure for vicious diseases such as tumors, we have synthesized and characterized a small series of new copper(ii) complexes with biologically important ligands such as acylpyruvate. In addition to this, we used another four copper(ii) complexes, with ligands of the same type to examine the antitumor potential. The antitumor potential of the copper(ii) complexes was examined on three tumor cell lines and one normal human cell line using the MTT assay. All seven tested complexes showed very good cytotoxic effects. Two copper complexes that showed the best antitumor potential were selected for further testing that showed the best potential for potential application in the future. The mechanism of activity of these complexes was examined in detail using tests such as cell cycle, ROS level, oxidative DNA damage, and proteins related to hypoxia analysis. In addition, we examined the binding abilities of these complexes with biomolecules (Guo, Ino, 5′-GMP, BSA, and DNA). The results showed that the tested compounds bind strongly to DNA molecules through intercalation. Also, it has been shown that the tested compounds adequately bind to the BSA molecule, which indicates an even greater potential for some future application of these compounds in clinical practice. © 2022 The Royal Society of Chemistry.
T2  - RSC Advances
T1  - Synthesis, characterization, antitumor potential, and investigation of mechanism of action of copper(ii) complexes with acylpyruvates as ligands: interactions with biomolecules and kinetic study
VL  - 12
IS  - 47
SP  - 30501
EP  - 30513
DO  - 10.1039/d2ra05797b
ER  - 

@article{
author = "Joksimović, Nenad and Petronijević, Jelena and Radisavljević, Snežana and Petrović, Biljana and Mihajlović, Kristina and Janković, Nenad and Milović, Emilija and Milivojević, Dušan and Ilić, Bojana and Đurić, Ana",
year = "2022",
abstract = "Considering the urgency of finding a cure for vicious diseases such as tumors, we have synthesized and characterized a small series of new copper(ii) complexes with biologically important ligands such as acylpyruvate. In addition to this, we used another four copper(ii) complexes, with ligands of the same type to examine the antitumor potential. The antitumor potential of the copper(ii) complexes was examined on three tumor cell lines and one normal human cell line using the MTT assay. All seven tested complexes showed very good cytotoxic effects. Two copper complexes that showed the best antitumor potential were selected for further testing that showed the best potential for potential application in the future. The mechanism of activity of these complexes was examined in detail using tests such as cell cycle, ROS level, oxidative DNA damage, and proteins related to hypoxia analysis. In addition, we examined the binding abilities of these complexes with biomolecules (Guo, Ino, 5′-GMP, BSA, and DNA). The results showed that the tested compounds bind strongly to DNA molecules through intercalation. Also, it has been shown that the tested compounds adequately bind to the BSA molecule, which indicates an even greater potential for some future application of these compounds in clinical practice. © 2022 The Royal Society of Chemistry.",
journal = "RSC Advances",
title = "Synthesis, characterization, antitumor potential, and investigation of mechanism of action of copper(ii) complexes with acylpyruvates as ligands: interactions with biomolecules and kinetic study",
volume = "12",
number = "47",
pages = "30501-30513",
doi = "10.1039/d2ra05797b"
}

Joksimović, N., Petronijević, J., Radisavljević, S., Petrović, B., Mihajlović, K., Janković, N., Milović, E., Milivojević, D., Ilić, B.,& Đurić, A.. (2022). Synthesis, characterization, antitumor potential, and investigation of mechanism of action of copper(ii) complexes with acylpyruvates as ligands: interactions with biomolecules and kinetic study. in RSC Advances, 12(47), 30501-30513.
https://doi.org/10.1039/d2ra05797b

Joksimović N, Petronijević J, Radisavljević S, Petrović B, Mihajlović K, Janković N, Milović E, Milivojević D, Ilić B, Đurić A. Synthesis, characterization, antitumor potential, and investigation of mechanism of action of copper(ii) complexes with acylpyruvates as ligands: interactions with biomolecules and kinetic study. in RSC Advances. 2022;12(47):30501-30513.
doi:10.1039/d2ra05797b .

Joksimović, Nenad, Petronijević, Jelena, Radisavljević, Snežana, Petrović, Biljana, Mihajlović, Kristina, Janković, Nenad, Milović, Emilija, Milivojević, Dušan, Ilić, Bojana, Đurić, Ana, "Synthesis, characterization, antitumor potential, and investigation of mechanism of action of copper(ii) complexes with acylpyruvates as ligands: interactions with biomolecules and kinetic study" in RSC Advances, 12, no. 47 (2022):30501-30513,
https://doi.org/10.1039/d2ra05797b . .

TY  - JOUR
AU  - Stepanović, Aleksandar
AU  - Nikitović, Marina
AU  - Stanojković, Tatjana P.
AU  - Grujičić, Danica
AU  - Bukumirić, Zoran
AU  - Srbljak, Ivana
AU  - Ilić, Rosanda
AU  - Milošević, Snežana
AU  - Arsenijević, Tatjana
AU  - Petrović, Nina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10265
AB  - A personalized approach to chemoradiation is important in reducing its potential side effects and identifying a group of patients prone to toxicity. MicroRNAs have been shown to have a predictive potential for radiotoxicity. The goal of the study was to test if levels of miRNA in peripheral blood mononuclear cells of glioblastoma patients are associated with toxicity and to identify the peak time point for toxicity. MicroRNA-10b/21/34a levels were measured in 43 patients with and without toxicity, at baseline, at the 15th, and at the 30th fraction by Real-Time quantitative Polymerase Chain Reaction. MicroRNA-10b/21 levels increased with toxicity grade (p = 0.014; p = 0.013); miR-21/34a levels were significantly different between patients with and without toxicity at the 15th fraction (p = 0.030; p = 0.045), while miR-34a levels significantly changed during treatment (p < 0.001). All three miRNAs showed a significantly high positive correlation with one another. MiR-34a might be considered as a predictive factor for toxicity due to its changes during treatment, and differences between the groups with and without toxicity; miR-10b might be used to predict toxicity; miR-10b/21 might be used for predicting the grade of toxicity in GB patients.
T2  - Scientific Reports
T1  - Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide
VL  - 12
IS  - 1
SP  - 7505
DO  - 10.1038/s41598-022-11445-9
ER  - 

@article{
author = "Stepanović, Aleksandar and Nikitović, Marina and Stanojković, Tatjana P. and Grujičić, Danica and Bukumirić, Zoran and Srbljak, Ivana and Ilić, Rosanda and Milošević, Snežana and Arsenijević, Tatjana and Petrović, Nina",
year = "2022",
abstract = "A personalized approach to chemoradiation is important in reducing its potential side effects and identifying a group of patients prone to toxicity. MicroRNAs have been shown to have a predictive potential for radiotoxicity. The goal of the study was to test if levels of miRNA in peripheral blood mononuclear cells of glioblastoma patients are associated with toxicity and to identify the peak time point for toxicity. MicroRNA-10b/21/34a levels were measured in 43 patients with and without toxicity, at baseline, at the 15th, and at the 30th fraction by Real-Time quantitative Polymerase Chain Reaction. MicroRNA-10b/21 levels increased with toxicity grade (p = 0.014; p = 0.013); miR-21/34a levels were significantly different between patients with and without toxicity at the 15th fraction (p = 0.030; p = 0.045), while miR-34a levels significantly changed during treatment (p < 0.001). All three miRNAs showed a significantly high positive correlation with one another. MiR-34a might be considered as a predictive factor for toxicity due to its changes during treatment, and differences between the groups with and without toxicity; miR-10b might be used to predict toxicity; miR-10b/21 might be used for predicting the grade of toxicity in GB patients.",
journal = "Scientific Reports",
title = "Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide",
volume = "12",
number = "1",
pages = "7505",
doi = "10.1038/s41598-022-11445-9"
}

Stepanović, A., Nikitović, M., Stanojković, T. P., Grujičić, D., Bukumirić, Z., Srbljak, I., Ilić, R., Milošević, S., Arsenijević, T.,& Petrović, N.. (2022). Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide. in Scientific Reports, 12(1), 7505.
https://doi.org/10.1038/s41598-022-11445-9

Stepanović A, Nikitović M, Stanojković TP, Grujičić D, Bukumirić Z, Srbljak I, Ilić R, Milošević S, Arsenijević T, Petrović N. Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide. in Scientific Reports. 2022;12(1):7505.
doi:10.1038/s41598-022-11445-9 .

Stepanović, Aleksandar, Nikitović, Marina, Stanojković, Tatjana P., Grujičić, Danica, Bukumirić, Zoran, Srbljak, Ivana, Ilić, Rosanda, Milošević, Snežana, Arsenijević, Tatjana, Petrović, Nina, "Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide" in Scientific Reports, 12, no. 1 (2022):7505,
https://doi.org/10.1038/s41598-022-11445-9 . .

TY  - JOUR
AU  - Mališić, Emina
AU  - Petrović, Nina
AU  - Brengues, Muriel
AU  - Azria, David
AU  - Matić, Ivana Z.
AU  - Srbljak Ćuk, Ivana
AU  - Kopčalić, Katarina
AU  - Stanojković, Tatjana P.
AU  - Nikitović, Marina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10544
AB  - The genetic background of each person might affect the severity of radiotherapy (RT)-induced normal tissue toxicity. The aim of study was to evaluate the influence of TGFB1 C-509T and Leu10Pro, XRCC1 Arg280His and XRCC3 Thr241Met polymorphisms as well as the level of radiation-induced CD8 T-lymphocyte apoptosis (RILA) on adverse effects of RT for prostate cancer (PCa). The study included 88 patients with localized or locally advanced PCa who were treated with RT. The polymorphisms were determined by PCR–RFLP analysis on DNA from peripheral blood mononuclear cells. RILA values were measured by flow cytometry. We found that CT genotype of TGFB1 C-509T could be protective biomarker for acute genitourinary (GU) and gastrointestinal (GI) radiotoxicity, while Thr variant of XRCC3 Thr241Met could predict the risk for acute GU radiotoxicity. Correlation between RILA values and toxicity was not detected. Univariate logistic regression analysis showed that Gleason score and risk group were risk factors for late GU, while for late GI radiotoxicity it was diabetes mellitus type 2. However, in multivariate model those were not proven to be significant and independent risk factors. Identification of assays combination predicting individual radiosensitivity is a crucial step towards personalized RT approach.
T2  - Scientific Reports
T1  - Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer
VL  - 12
IS  - 1
SP  - 21306
DO  - 10.1038/s41598-022-25328-6
ER  - 

@article{
author = "Mališić, Emina and Petrović, Nina and Brengues, Muriel and Azria, David and Matić, Ivana Z. and Srbljak Ćuk, Ivana and Kopčalić, Katarina and Stanojković, Tatjana P. and Nikitović, Marina",
year = "2022",
abstract = "The genetic background of each person might affect the severity of radiotherapy (RT)-induced normal tissue toxicity. The aim of study was to evaluate the influence of TGFB1 C-509T and Leu10Pro, XRCC1 Arg280His and XRCC3 Thr241Met polymorphisms as well as the level of radiation-induced CD8 T-lymphocyte apoptosis (RILA) on adverse effects of RT for prostate cancer (PCa). The study included 88 patients with localized or locally advanced PCa who were treated with RT. The polymorphisms were determined by PCR–RFLP analysis on DNA from peripheral blood mononuclear cells. RILA values were measured by flow cytometry. We found that CT genotype of TGFB1 C-509T could be protective biomarker for acute genitourinary (GU) and gastrointestinal (GI) radiotoxicity, while Thr variant of XRCC3 Thr241Met could predict the risk for acute GU radiotoxicity. Correlation between RILA values and toxicity was not detected. Univariate logistic regression analysis showed that Gleason score and risk group were risk factors for late GU, while for late GI radiotoxicity it was diabetes mellitus type 2. However, in multivariate model those were not proven to be significant and independent risk factors. Identification of assays combination predicting individual radiosensitivity is a crucial step towards personalized RT approach.",
journal = "Scientific Reports",
title = "Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer",
volume = "12",
number = "1",
pages = "21306",
doi = "10.1038/s41598-022-25328-6"
}

Mališić, E., Petrović, N., Brengues, M., Azria, D., Matić, I. Z., Srbljak Ćuk, I., Kopčalić, K., Stanojković, T. P.,& Nikitović, M.. (2022). Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer. in Scientific Reports, 12(1), 21306.
https://doi.org/10.1038/s41598-022-25328-6

Mališić E, Petrović N, Brengues M, Azria D, Matić IZ, Srbljak Ćuk I, Kopčalić K, Stanojković TP, Nikitović M. Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer. in Scientific Reports. 2022;12(1):21306.
doi:10.1038/s41598-022-25328-6 .

Mališić, Emina, Petrović, Nina, Brengues, Muriel, Azria, David, Matić, Ivana Z., Srbljak Ćuk, Ivana, Kopčalić, Katarina, Stanojković, Tatjana P., Nikitović, Marina, "Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer" in Scientific Reports, 12, no. 1 (2022):21306,
https://doi.org/10.1038/s41598-022-25328-6 . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Ergün, Sercan
AU  - Đorđić-Crnogorac, Marija
AU  - Stanojković, Tatjana
AU  - Mališić, Emina
AU  - Matić Ivana Z.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10706
AB  - Phytochemicals and bioactive substances derived from a wide range of plant extracts have been reported to exert various anticancer effects. Prostate cancer is one of the leading causes of cancer-related deaths within the male population. Prostate cancer-specific miRNA signatures were associated with cancer formation and progression, with various subtypes, and response to therapy. MicroRNA levels of expression were shown to change after the treatment of various compounds and substances extracted from natural products. Natural herbal compounds were shown to induce variations in miRNA expression levels in cancer cells. The aims of this study were to investigate the cytotoxic effects of methanol, ethyl-acetate, and hexane extracts obtained from branch-body part and flowers of Hypericum perforatum L. against humane PC-3 and DU 145 and to test potential miRNA-128/133b/155/193a/206/21/335 signature changes and differences between the two prostate cancer cell lines. Cytotoxic activity of H. perforatum extracts, their effects on cell cycle distribution, and miRNA expression levels were examined in humane PC-3 and DU 145 prostate cancer cells by MTT cell survival assay, flow cytometry, and quantitative real-time PCR. Hexane extract of flowers showed the strongest intensity of cytotoxic activity against PC-3 and DU 145 cells. The highest increase in the percentage of PC-3 cells in the subG1 phase was observed in cell samples treated with hexane extract of flowers and branch-body part. Significant differences in miRNA-128/133b/155/193a/206/21/335 levels were observed between PC-3 and DU 145 cell lines, especially in samples treated with flower extracts compared with the branch-body part. Conclusions: Investigated extracts have significant anticancer potential not only from the aspects of cytotoxicity and cell cycle effects but also from the aspect of lowering oncogenic or increasing tumor-suppressive miRNAs. The best effect might be the increase of tumor-suppressive miR-128 (accompanied by miR-193a) induced by the hexane extract of the flowers, which also exerted the highest cytotoxic activity. Hexane extract of flowers may be the candidate for further investigation for improving the efficiency of standard therapies for PCa. A miRNA signature might be cell-type specific after the treatment with H. perforatum extracts.
T2  - Genetika
T1  - Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells
VL  - 54
IS  - 3
SP  - 1249
EP  - 1270
DO  - 10.2298/GENSR2203249P
ER  - 

@article{
author = "Petrović, Nina and Ergün, Sercan and Đorđić-Crnogorac, Marija and Stanojković, Tatjana and Mališić, Emina and Matić Ivana Z.",
year = "2022",
abstract = "Phytochemicals and bioactive substances derived from a wide range of plant extracts have been reported to exert various anticancer effects. Prostate cancer is one of the leading causes of cancer-related deaths within the male population. Prostate cancer-specific miRNA signatures were associated with cancer formation and progression, with various subtypes, and response to therapy. MicroRNA levels of expression were shown to change after the treatment of various compounds and substances extracted from natural products. Natural herbal compounds were shown to induce variations in miRNA expression levels in cancer cells. The aims of this study were to investigate the cytotoxic effects of methanol, ethyl-acetate, and hexane extracts obtained from branch-body part and flowers of Hypericum perforatum L. against humane PC-3 and DU 145 and to test potential miRNA-128/133b/155/193a/206/21/335 signature changes and differences between the two prostate cancer cell lines. Cytotoxic activity of H. perforatum extracts, their effects on cell cycle distribution, and miRNA expression levels were examined in humane PC-3 and DU 145 prostate cancer cells by MTT cell survival assay, flow cytometry, and quantitative real-time PCR. Hexane extract of flowers showed the strongest intensity of cytotoxic activity against PC-3 and DU 145 cells. The highest increase in the percentage of PC-3 cells in the subG1 phase was observed in cell samples treated with hexane extract of flowers and branch-body part. Significant differences in miRNA-128/133b/155/193a/206/21/335 levels were observed between PC-3 and DU 145 cell lines, especially in samples treated with flower extracts compared with the branch-body part. Conclusions: Investigated extracts have significant anticancer potential not only from the aspects of cytotoxicity and cell cycle effects but also from the aspect of lowering oncogenic or increasing tumor-suppressive miRNAs. The best effect might be the increase of tumor-suppressive miR-128 (accompanied by miR-193a) induced by the hexane extract of the flowers, which also exerted the highest cytotoxic activity. Hexane extract of flowers may be the candidate for further investigation for improving the efficiency of standard therapies for PCa. A miRNA signature might be cell-type specific after the treatment with H. perforatum extracts.",
journal = "Genetika",
title = "Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells",
volume = "54",
number = "3",
pages = "1249-1270",
doi = "10.2298/GENSR2203249P"
}

Petrović, N., Ergün, S., Đorđić-Crnogorac, M., Stanojković, T., Mališić, E.,& Matić Ivana Z.. (2022). Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells. in Genetika, 54(3), 1249-1270.
https://doi.org/10.2298/GENSR2203249P

Petrović N, Ergün S, Đorđić-Crnogorac M, Stanojković T, Mališić E, Matić Ivana Z.. Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells. in Genetika. 2022;54(3):1249-1270.
doi:10.2298/GENSR2203249P .

Petrović, Nina, Ergün, Sercan, Đorđić-Crnogorac, Marija, Stanojković, Tatjana, Mališić, Emina, Matić Ivana Z., "Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells" in Genetika, 54, no. 3 (2022):1249-1270,
https://doi.org/10.2298/GENSR2203249P . .

TY  - JOUR
AU  - Srdić-Rajić, Tatjana
AU  - Metlaš, Radmila
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10137
AB  - Gene as the basic functional unit of DNA encodes information about the product such as protein. The majority of proteins realize function through protein-protein interactions involving short protein motifs. However, some proteins such as antibodies are established by the rearrangement of several (V-D-J) gene segments with the potential addition of nontemplated nucleotides that may change information encoded by the respective gene segment used. Antibody VH domain sequence analysis by ISM bioinformatics approach that is based on amino acids physicochemical features, enable to distinguish the contribution of the information encoded by VH gene or generated during VDJ gene recombination for antibody-antigen interaction. The data presented in this report revealed the significance of CDRH3 for the interaction of antibody specific for immunogenic molecules while CDRH3 contribution is minor for antibody interaction with nonimmunogenic molecules such as haptens and native mammalian dsDNA. Thus, paratopes might be located in the CDRH3 or VH regions.
T2  - Immunology Letters
T1  - Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location
VL  - 241
SP  - 55
EP  - 57
DO  - 10.1016/j.imlet.2021.11.003
ER  - 

@article{
author = "Srdić-Rajić, Tatjana and Metlaš, Radmila",
year = "2022",
abstract = "Gene as the basic functional unit of DNA encodes information about the product such as protein. The majority of proteins realize function through protein-protein interactions involving short protein motifs. However, some proteins such as antibodies are established by the rearrangement of several (V-D-J) gene segments with the potential addition of nontemplated nucleotides that may change information encoded by the respective gene segment used. Antibody VH domain sequence analysis by ISM bioinformatics approach that is based on amino acids physicochemical features, enable to distinguish the contribution of the information encoded by VH gene or generated during VDJ gene recombination for antibody-antigen interaction. The data presented in this report revealed the significance of CDRH3 for the interaction of antibody specific for immunogenic molecules while CDRH3 contribution is minor for antibody interaction with nonimmunogenic molecules such as haptens and native mammalian dsDNA. Thus, paratopes might be located in the CDRH3 or VH regions.",
journal = "Immunology Letters",
title = "Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location",
volume = "241",
pages = "55-57",
doi = "10.1016/j.imlet.2021.11.003"
}

Srdić-Rajić, T.,& Metlaš, R.. (2022). Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location. in Immunology Letters, 241, 55-57.
https://doi.org/10.1016/j.imlet.2021.11.003

Srdić-Rajić T, Metlaš R. Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location. in Immunology Letters. 2022;241:55-57.
doi:10.1016/j.imlet.2021.11.003 .

Srdić-Rajić, Tatjana, Metlaš, Radmila, "Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location" in Immunology Letters, 241 (2022):55-57,
https://doi.org/10.1016/j.imlet.2021.11.003 . .

TY  - JOUR
AU  - Milovanović, Jelena
AU  - Todorović-Raković, Nataša
AU  - Vujasinović, Tijana
AU  - Greenman, John
AU  - Mandušić, Vesna
AU  - Radulović, Marko
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10392
AB  - Background Granulysin (GNLY) is a cytolytic and proinflammatory molecule which also acts as an immune alarmin. The multifunctional nature of this molecule has made it challenging to define its full potential as a biomarker in breast cancer.AimTo evaluate the prognostic value of intratumoral GNLY in primary breast cancer patients and its association with established clinicopathological parameters.Patients and methodsThe study included 69 node-negative breast cancer patients with known clinicopathological parameters, all of whom had not received any prior hormonal or chemotherapeutic systemic therapy that would interfere with the course of disease. The median follow-up period was 144 months. Steroid hormone receptor status was determined by ligand-binding assay and HER2 status by chromogenic in situ hybridisation (CISH). Intratumoral GNLY mRNA levels were determined by RT-qPCR. Prognostic performance was evaluated by the receiver operating characteristic (ROC), Cox proportional hazards regression and Kaplan-Meier analysis. Classification of patients into GNLYlow and GNLYhigh subgroups was performed by the use of the outcome-oriented cut-off point categorisation approach.ResultsThere was a significant difference between GNLY values of patients without any recurrences and those with local or distant recurrences (Mann-Whitney test, p = 0.05 and p = 0.02, respectively). None of the tested parameters showed prognostic significance for local and distant recurrences when combined. When distant metastases and local recurrences were separated as events, the best prognostic performance was observed for GNLY as compared with any clinicopathological parameter (AUC=0.24 and p = 0.04 for local events; AUC=0.71 and p = 0.03 for distant events). Local recurrence incidence was 0% for the GNLYhigh subgroup and 19% for the GNLYlow subgroup; however distant recurrence incidence was 24% for the GNLYhigh subgroup but only 3% for the GNLYlow subgroup (Kaplan–Meier analysis). A significant positive correlation was found between intratumoral ER and GNLY levels, and a significant negative correlation between tumour grade and GNLY levels.ConclusionHigh levels of granulysin prognosticate low risk of local recurrence but a high risk of distant metastasis in primary, untreated, breast cancer patients.
T2  - Pathology - Research and Practice
T1  - Can granulysin provide prognostic value in primary breast cancer?
VL  - 237
SP  - 154039
DO  - 10.1016/j.prp.2022.154039
ER  - 

@article{
author = "Milovanović, Jelena and Todorović-Raković, Nataša and Vujasinović, Tijana and Greenman, John and Mandušić, Vesna and Radulović, Marko",
year = "2022",
abstract = "Background Granulysin (GNLY) is a cytolytic and proinflammatory molecule which also acts as an immune alarmin. The multifunctional nature of this molecule has made it challenging to define its full potential as a biomarker in breast cancer.AimTo evaluate the prognostic value of intratumoral GNLY in primary breast cancer patients and its association with established clinicopathological parameters.Patients and methodsThe study included 69 node-negative breast cancer patients with known clinicopathological parameters, all of whom had not received any prior hormonal or chemotherapeutic systemic therapy that would interfere with the course of disease. The median follow-up period was 144 months. Steroid hormone receptor status was determined by ligand-binding assay and HER2 status by chromogenic in situ hybridisation (CISH). Intratumoral GNLY mRNA levels were determined by RT-qPCR. Prognostic performance was evaluated by the receiver operating characteristic (ROC), Cox proportional hazards regression and Kaplan-Meier analysis. Classification of patients into GNLYlow and GNLYhigh subgroups was performed by the use of the outcome-oriented cut-off point categorisation approach.ResultsThere was a significant difference between GNLY values of patients without any recurrences and those with local or distant recurrences (Mann-Whitney test, p = 0.05 and p = 0.02, respectively). None of the tested parameters showed prognostic significance for local and distant recurrences when combined. When distant metastases and local recurrences were separated as events, the best prognostic performance was observed for GNLY as compared with any clinicopathological parameter (AUC=0.24 and p = 0.04 for local events; AUC=0.71 and p = 0.03 for distant events). Local recurrence incidence was 0% for the GNLYhigh subgroup and 19% for the GNLYlow subgroup; however distant recurrence incidence was 24% for the GNLYhigh subgroup but only 3% for the GNLYlow subgroup (Kaplan–Meier analysis). A significant positive correlation was found between intratumoral ER and GNLY levels, and a significant negative correlation between tumour grade and GNLY levels.ConclusionHigh levels of granulysin prognosticate low risk of local recurrence but a high risk of distant metastasis in primary, untreated, breast cancer patients.",
journal = "Pathology - Research and Practice",
title = "Can granulysin provide prognostic value in primary breast cancer?",
volume = "237",
pages = "154039",
doi = "10.1016/j.prp.2022.154039"
}

Milovanović, J., Todorović-Raković, N., Vujasinović, T., Greenman, J., Mandušić, V.,& Radulović, M.. (2022). Can granulysin provide prognostic value in primary breast cancer?. in Pathology - Research and Practice, 237, 154039.
https://doi.org/10.1016/j.prp.2022.154039

Milovanović J, Todorović-Raković N, Vujasinović T, Greenman J, Mandušić V, Radulović M. Can granulysin provide prognostic value in primary breast cancer?. in Pathology - Research and Practice. 2022;237:154039.
doi:10.1016/j.prp.2022.154039 .

Milovanović, Jelena, Todorović-Raković, Nataša, Vujasinović, Tijana, Greenman, John, Mandušić, Vesna, Radulović, Marko, "Can granulysin provide prognostic value in primary breast cancer?" in Pathology - Research and Practice, 237 (2022):154039,
https://doi.org/10.1016/j.prp.2022.154039 . .

TY  - JOUR
AU  - Tadić, Julijana D.
AU  - Lađarević, Jelena M.
AU  - Vitnik, Željko J.
AU  - Vitnik, Vesna D.
AU  - Stanojković, Tatjana P.
AU  - Matić, Ivana Z.
AU  - Mijin, Dušan Ž.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10890
AB  - Seven novel azo dyes with 2-pyridone and dihydropyrimidinone moieties have been synthesized and thoroughly characterized. The azo-hydrazone tautomerism has been investigated by experimental and theoretical approaches. The optimizations of geometries have been performed with density functional theory (DFT). The vibrational and NMR spectra were calculated and correlated with experimental ones. Furthermore, quantum chemical descriptors were calculated and MEP maps were plotted to determine biological reactivity of dyes. The antioxidant assay evinced that 5, 6 and 7 are promising antioxidant candidates. In vitro cytotoxic activity was studied against three malignant cell lines: prostate adenocarcinoma (PC-3), lung carcinoma (A549) and chronic myelogenous leukemia (K562), as well as against human normal lung fibroblasts (MRC-5), using MTT assay. Examination of cytotoxic effects on human cancer cell lines showed the concentration dependent cytotoxicity of all investigated compounds. The K562 cells were the most sensitive to the cytotoxicity of the compounds 3, 5 and 6, wherein compound 5 was particularly prominent and selective in cytotoxic action between K562 (24.97 μM) and PC-3 (48.98 μM) cancer cells, and normal MRC-5 (91.11 μM) cells. Moreover, the cell cycle analysis of compound 5 was examined in K562 cells, by flow cytometry, to study its mechanism of anticancer action. Finally, in silico evaluation of physicochemical parameters, druglikeness and ADME properties showed that investigated compounds are orally bioavailable with no permeation to the blood brain barrier.
T2  - Dyes and Pigments
T1  - Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity
VL  - 187
SP  - 109123
DO  - 10.1016/j.dyepig.2020.109123
ER  - 

@article{
author = "Tadić, Julijana D. and Lađarević, Jelena M. and Vitnik, Željko J. and Vitnik, Vesna D. and Stanojković, Tatjana P. and Matić, Ivana Z. and Mijin, Dušan Ž.",
year = "2021",
abstract = "Seven novel azo dyes with 2-pyridone and dihydropyrimidinone moieties have been synthesized and thoroughly characterized. The azo-hydrazone tautomerism has been investigated by experimental and theoretical approaches. The optimizations of geometries have been performed with density functional theory (DFT). The vibrational and NMR spectra were calculated and correlated with experimental ones. Furthermore, quantum chemical descriptors were calculated and MEP maps were plotted to determine biological reactivity of dyes. The antioxidant assay evinced that 5, 6 and 7 are promising antioxidant candidates. In vitro cytotoxic activity was studied against three malignant cell lines: prostate adenocarcinoma (PC-3), lung carcinoma (A549) and chronic myelogenous leukemia (K562), as well as against human normal lung fibroblasts (MRC-5), using MTT assay. Examination of cytotoxic effects on human cancer cell lines showed the concentration dependent cytotoxicity of all investigated compounds. The K562 cells were the most sensitive to the cytotoxicity of the compounds 3, 5 and 6, wherein compound 5 was particularly prominent and selective in cytotoxic action between K562 (24.97 μM) and PC-3 (48.98 μM) cancer cells, and normal MRC-5 (91.11 μM) cells. Moreover, the cell cycle analysis of compound 5 was examined in K562 cells, by flow cytometry, to study its mechanism of anticancer action. Finally, in silico evaluation of physicochemical parameters, druglikeness and ADME properties showed that investigated compounds are orally bioavailable with no permeation to the blood brain barrier.",
journal = "Dyes and Pigments",
title = "Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity",
volume = "187",
pages = "109123",
doi = "10.1016/j.dyepig.2020.109123"
}

Tadić, J. D., Lađarević, J. M., Vitnik, Ž. J., Vitnik, V. D., Stanojković, T. P., Matić, I. Z.,& Mijin, D. Ž.. (2021). Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity. in Dyes and Pigments, 187, 109123.
https://doi.org/10.1016/j.dyepig.2020.109123

Tadić JD, Lađarević JM, Vitnik ŽJ, Vitnik VD, Stanojković TP, Matić IZ, Mijin DŽ. Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity. in Dyes and Pigments. 2021;187:109123.
doi:10.1016/j.dyepig.2020.109123 .

Tadić, Julijana D., Lađarević, Jelena M., Vitnik, Željko J., Vitnik, Vesna D., Stanojković, Tatjana P., Matić, Ivana Z., Mijin, Dušan Ž., "Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity" in Dyes and Pigments, 187 (2021):109123,
https://doi.org/10.1016/j.dyepig.2020.109123 . .

TY  - JOUR
AU  - Matić, Ivana Z.
AU  - Ergün, Sercan
AU  - Đorđić Crnogorac, Marija
AU  - Misir, Sema
AU  - Aliyazicioğlu, Yüksel
AU  - Damjanović, Ana
AU  - Džudžević-Čančar, Hurija
AU  - Stanojković, Tatjana P.
AU  - Konanç, Kalbiye
AU  - Petrović, Nina
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9406
AB  - Six extracts were obtained from plant species Hypericum perforatum L., collected at Samsun in Turkey. The aim of this study was to examine the mechanisms of the anticancer activity of these extracts. Methanol, ethyl-acetate and hexane were used as a solvents for extraction from both branch-body part of the plant (extracts 1, 2 and 3) and from plant flowers (extracts 4, 5 and 6). The cytotoxic effects of the extracts were determined against 2D and 3D cancer cell models. Cell cycle changes of treated HeLa cells were analyzed by flow cytometry. Measurements of gene and microRNA expression levels in treated HeLa cells were done by quantitative real time PCR. Five examined extracts (2–6) exerted selective concentration-dependent cytotoxic effects on HeLa, K562, and A549 cancer cells, while the extract 1 exhibited very weak cytotoxicity. The extract 6 showed the highest intensity of cytotoxic activity. All tested extracts (2–6) demonstrated the ability to induce apoptosis in HeLa cells through activation of caspase-3. These extracts remarkably decreased gene expression levels of MMP2, MMP9, TIMP3, and VEGFA in HeLa cells. Flower extracts might have stronger effects on miR128/193a-5p/335 level changes than branch-body extracts. Hypericum perforatum extracts exerted weaker cytotoxic effects on 3D HeLa spheroids when compared with their effects on 2D monolayer HeLa cells. Taken together, results of our research may suggest the promising anticancer properties of the Hypericum perforatum extracts. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
T2  - Cytotechnology
T1  - Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models
VL  - 73
IS  - 3
SP  - 373
EP  - 389
DO  - 10.1007/s10616-021-00464-5
ER  - 

@article{
author = "Matić, Ivana Z. and Ergün, Sercan and Đorđić Crnogorac, Marija and Misir, Sema and Aliyazicioğlu, Yüksel and Damjanović, Ana and Džudžević-Čančar, Hurija and Stanojković, Tatjana P. and Konanç, Kalbiye and Petrović, Nina",
year = "2021",
abstract = "Six extracts were obtained from plant species Hypericum perforatum L., collected at Samsun in Turkey. The aim of this study was to examine the mechanisms of the anticancer activity of these extracts. Methanol, ethyl-acetate and hexane were used as a solvents for extraction from both branch-body part of the plant (extracts 1, 2 and 3) and from plant flowers (extracts 4, 5 and 6). The cytotoxic effects of the extracts were determined against 2D and 3D cancer cell models. Cell cycle changes of treated HeLa cells were analyzed by flow cytometry. Measurements of gene and microRNA expression levels in treated HeLa cells were done by quantitative real time PCR. Five examined extracts (2–6) exerted selective concentration-dependent cytotoxic effects on HeLa, K562, and A549 cancer cells, while the extract 1 exhibited very weak cytotoxicity. The extract 6 showed the highest intensity of cytotoxic activity. All tested extracts (2–6) demonstrated the ability to induce apoptosis in HeLa cells through activation of caspase-3. These extracts remarkably decreased gene expression levels of MMP2, MMP9, TIMP3, and VEGFA in HeLa cells. Flower extracts might have stronger effects on miR128/193a-5p/335 level changes than branch-body extracts. Hypericum perforatum extracts exerted weaker cytotoxic effects on 3D HeLa spheroids when compared with their effects on 2D monolayer HeLa cells. Taken together, results of our research may suggest the promising anticancer properties of the Hypericum perforatum extracts. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.",
journal = "Cytotechnology",
title = "Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models",
volume = "73",
number = "3",
pages = "373-389",
doi = "10.1007/s10616-021-00464-5"
}

Matić, I. Z., Ergün, S., Đorđić Crnogorac, M., Misir, S., Aliyazicioğlu, Y., Damjanović, A., Džudžević-Čančar, H., Stanojković, T. P., Konanç, K.,& Petrović, N.. (2021). Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models. in Cytotechnology, 73(3), 373-389.
https://doi.org/10.1007/s10616-021-00464-5

Matić IZ, Ergün S, Đorđić Crnogorac M, Misir S, Aliyazicioğlu Y, Damjanović A, Džudžević-Čančar H, Stanojković TP, Konanç K, Petrović N. Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models. in Cytotechnology. 2021;73(3):373-389.
doi:10.1007/s10616-021-00464-5 .

Matić, Ivana Z., Ergün, Sercan, Đorđić Crnogorac, Marija, Misir, Sema, Aliyazicioğlu, Yüksel, Damjanović, Ana, Džudžević-Čančar, Hurija, Stanojković, Tatjana P., Konanç, Kalbiye, Petrović, Nina, "Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models" in Cytotechnology, 73, no. 3 (2021):373-389,
https://doi.org/10.1007/s10616-021-00464-5 . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Nakashidze, Irina
AU  - Nedeljković, Milica
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9555
AB  - Breast cancer (BC) is a leading cause of death among women with malignant diseases. The selection of adequate therapies for highly invasive and metastatic BCs still represents a major challenge. Novel combinatorial therapeutic approaches are urgently required to enhance the efficiency of BC treatment. Recently, microRNAs (miRNAs) emerged as key regulators of the complex mechanisms that govern BC therapeutic resistance and susceptibility. In the present review we aim to critically examine how miRNAs influence BC response to therapies, or how to use miRNAs as a basis for new therapeutic approaches. We summarized recent findings in this rapidly evolving field, emphasizing the challenges still ahead for the successful implementation of miRNAs into BC treatment while providing insights for future BC management. The goal of this review was to propose miRNAs, that might simultaneously improve the efficacy of all four therapies that are the backbone of current BC management (radio-, chemo-, targeted, and hormone therapy). Among the fifty-nine described miRNAs, miR-21 and miR-16 emerged as the most promising, closely followed by miR-205, miR-451, miR-182, and miRNAs from the let-7 family. miR-21 inhibition might be the best choice for future improvement of invasive BC treatment. New therapeutic strategies of miRNA-based agents alongside current standard treatment modalities could greatly benefit BC patients. This review represents a guideline on how to navigate this elaborate puzzle. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.
T2  - Journal of Mammary Gland Biology and Neoplasia
T1  - Breast Cancer Response to Therapy: Can microRNAs Lead the Way?
VL  - 26
IS  - 2
SP  - 157
EP  - 178
DO  - 10.1007/s10911-021-09478-3
ER  - 

@article{
author = "Petrović, Nina and Nakashidze, Irina and Nedeljković, Milica",
year = "2021",
abstract = "Breast cancer (BC) is a leading cause of death among women with malignant diseases. The selection of adequate therapies for highly invasive and metastatic BCs still represents a major challenge. Novel combinatorial therapeutic approaches are urgently required to enhance the efficiency of BC treatment. Recently, microRNAs (miRNAs) emerged as key regulators of the complex mechanisms that govern BC therapeutic resistance and susceptibility. In the present review we aim to critically examine how miRNAs influence BC response to therapies, or how to use miRNAs as a basis for new therapeutic approaches. We summarized recent findings in this rapidly evolving field, emphasizing the challenges still ahead for the successful implementation of miRNAs into BC treatment while providing insights for future BC management. The goal of this review was to propose miRNAs, that might simultaneously improve the efficacy of all four therapies that are the backbone of current BC management (radio-, chemo-, targeted, and hormone therapy). Among the fifty-nine described miRNAs, miR-21 and miR-16 emerged as the most promising, closely followed by miR-205, miR-451, miR-182, and miRNAs from the let-7 family. miR-21 inhibition might be the best choice for future improvement of invasive BC treatment. New therapeutic strategies of miRNA-based agents alongside current standard treatment modalities could greatly benefit BC patients. This review represents a guideline on how to navigate this elaborate puzzle. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.",
journal = "Journal of Mammary Gland Biology and Neoplasia",
title = "Breast Cancer Response to Therapy: Can microRNAs Lead the Way?",
volume = "26",
number = "2",
pages = "157-178",
doi = "10.1007/s10911-021-09478-3"
}

Petrović, N., Nakashidze, I.,& Nedeljković, M.. (2021). Breast Cancer Response to Therapy: Can microRNAs Lead the Way?. in Journal of Mammary Gland Biology and Neoplasia, 26(2), 157-178.
https://doi.org/10.1007/s10911-021-09478-3

Petrović N, Nakashidze I, Nedeljković M. Breast Cancer Response to Therapy: Can microRNAs Lead the Way?. in Journal of Mammary Gland Biology and Neoplasia. 2021;26(2):157-178.
doi:10.1007/s10911-021-09478-3 .

Petrović, Nina, Nakashidze, Irina, Nedeljković, Milica, "Breast Cancer Response to Therapy: Can microRNAs Lead the Way?" in Journal of Mammary Gland Biology and Neoplasia, 26, no. 2 (2021):157-178,
https://doi.org/10.1007/s10911-021-09478-3 . .

TY  - JOUR
AU  - Stanojković, Tatjana P.
AU  - Matić, Ivana Z.
AU  - Petrović, Nina
AU  - Stanković, Vesna
AU  - Kopčalić, Katarina
AU  - Besu, Irina
AU  - Đorđić Crnogorac, Marija
AU  - Mališić, Emina
AU  - Mirjačić-Martinović, Katarina
AU  - Vuletić, Ana
AU  - Bukumirić, Zoran
AU  - Žižak, Željko
AU  - Veldwijk, Marlon
AU  - Herskind, Carsten
AU  - Nikitović, Marina
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9713
AB  - One of the challenges of radiation oncology in the era of personalized medicine is identification of biomarkers associated with individual radiosensitivity. The aim of research was to evaluate the possible clinical value of the associations between clinical, physical, and biological factors, and risk for development of acute radiotoxicity in patients with prostate cancer. The study involved forty four patients treated with three-dimensional conformal radiotherapy. The concentrations of IL-1β, IL-2, IL-6, IFN-γ and TGF-β1 were assessed before radiotherapy, after 5th, 15th and 25th radiotherapy fractions, at the end, and 1 month after the end of radiotherapy. Cytokine gene expression was determined in peripheral blood mononuclear cells. The univariate analysis of circulating cytokine levels during radiotherapy showed that increased serum concentrations of IL-6 were significantly associated with higher grade of acute genitourinary toxicity. The multivariate analysis demonstrated that increased level of IL-6 during the radiotherapy was significantly associated with higher grade of acute genitourinary toxicity across treatment. TGF-β expression levels significantly decreased during course of radiotherapy. Research indicates that changes in circulating cytokine levels might be important parameter of radiotoxicity in patients with prostate cancer. These findings suggest that future studies based on multi-parameter examination are necessary for prediction of individual radiosensitivity.
T2  - Scientific Reports
T1  - Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients
VL  - 10
IS  - 1
SP  - 19002
DO  - 10.1038/s41598-020-75812-0
ER  - 

@article{
author = "Stanojković, Tatjana P. and Matić, Ivana Z. and Petrović, Nina and Stanković, Vesna and Kopčalić, Katarina and Besu, Irina and Đorđić Crnogorac, Marija and Mališić, Emina and Mirjačić-Martinović, Katarina and Vuletić, Ana and Bukumirić, Zoran and Žižak, Željko and Veldwijk, Marlon and Herskind, Carsten and Nikitović, Marina",
year = "2020",
abstract = "One of the challenges of radiation oncology in the era of personalized medicine is identification of biomarkers associated with individual radiosensitivity. The aim of research was to evaluate the possible clinical value of the associations between clinical, physical, and biological factors, and risk for development of acute radiotoxicity in patients with prostate cancer. The study involved forty four patients treated with three-dimensional conformal radiotherapy. The concentrations of IL-1β, IL-2, IL-6, IFN-γ and TGF-β1 were assessed before radiotherapy, after 5th, 15th and 25th radiotherapy fractions, at the end, and 1 month after the end of radiotherapy. Cytokine gene expression was determined in peripheral blood mononuclear cells. The univariate analysis of circulating cytokine levels during radiotherapy showed that increased serum concentrations of IL-6 were significantly associated with higher grade of acute genitourinary toxicity. The multivariate analysis demonstrated that increased level of IL-6 during the radiotherapy was significantly associated with higher grade of acute genitourinary toxicity across treatment. TGF-β expression levels significantly decreased during course of radiotherapy. Research indicates that changes in circulating cytokine levels might be important parameter of radiotoxicity in patients with prostate cancer. These findings suggest that future studies based on multi-parameter examination are necessary for prediction of individual radiosensitivity.",
journal = "Scientific Reports",
title = "Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients",
volume = "10",
number = "1",
pages = "19002",
doi = "10.1038/s41598-020-75812-0"
}

Stanojković, T. P., Matić, I. Z., Petrović, N., Stanković, V., Kopčalić, K., Besu, I., Đorđić Crnogorac, M., Mališić, E., Mirjačić-Martinović, K., Vuletić, A., Bukumirić, Z., Žižak, Ž., Veldwijk, M., Herskind, C.,& Nikitović, M.. (2020). Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients. in Scientific Reports, 10(1), 19002.
https://doi.org/10.1038/s41598-020-75812-0

Stanojković TP, Matić IZ, Petrović N, Stanković V, Kopčalić K, Besu I, Đorđić Crnogorac M, Mališić E, Mirjačić-Martinović K, Vuletić A, Bukumirić Z, Žižak Ž, Veldwijk M, Herskind C, Nikitović M. Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients. in Scientific Reports. 2020;10(1):19002.
doi:10.1038/s41598-020-75812-0 .

Stanojković, Tatjana P., Matić, Ivana Z., Petrović, Nina, Stanković, Vesna, Kopčalić, Katarina, Besu, Irina, Đorđić Crnogorac, Marija, Mališić, Emina, Mirjačić-Martinović, Katarina, Vuletić, Ana, Bukumirić, Zoran, Žižak, Željko, Veldwijk, Marlon, Herskind, Carsten, Nikitović, Marina, "Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients" in Scientific Reports, 10, no. 1 (2020):19002,
https://doi.org/10.1038/s41598-020-75812-0 . .