TY  - JOUR
AU  - Mićović, Žarko
AU  - Kostić, Sanja
AU  - Mutavdžin, Slavica
AU  - Andrejević, Aleksa
AU  - Stamenković, Aleksandra
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
AU  - Đurić, Marko
AU  - Hrnčić, Dragan
AU  - Živković, Vladimir
AU  - Jakovljević, Vladimir
AU  - Đurić, Dragan
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12970
AB  - Background/Aim. Chronically induced hypermethioninemia leads to hyperhomocysteinemia which causes oxidative stress, atherogenesis, neurodegeneration and cancer. However, little is known about the acute and subchronic effects of DL-methionine (Met). The aim of study was to assess the effects of acutely and subchronically applied Met on oxidative stress parameters in rat plasma [enzymes: catalase (CAT), glutathione peroxidise (GPx), superoxide dismutase (SOD) and index of lipid peroxidation, malondialdehyde (MDA)], and acetylcholinesterase (AChE) activity in rat cardiac tissue. Methods. The enzymes activities, as well as MDA concentration were evaluated following acute (n = 8) and subchronic (n = 10) application of Met [i.p. 0.8 mmoL/kg body weight (b.w.) in a single dose in the acute overload or daily during three weeks in the subchronic overload]. The same was done in the control groups following application of physiological solution [i.p. 1 mL 0.9% NaCl (n = 8) in the acute overload and 0.1–0.2 mL 0.9% NaCl, daily during three weeks (n =10) in the subchronic overload]. Tested parameters were evaluated 60 minutes after application in acute experiments and after three weeks of treatment in subchronic experiments. Results. There were no difference in homocysteine values between the groups treated with Met for three weeks and the control group. Met administration significantly increased the activity of CAT and GPx after 1 h compared to the control group (p = 0.008 for both enzymes), whereas the activity of SOD and MDA concentrations were unchanged. Subchronically applied Met did not affect activity of antioxidant enzymes and MDA level. AChE activity did not show any change in rat cardiac tissue after 1 h, but it was significantly decreased after the subchronic treatment (p = 0.041). Conclusion. Results of present research indicate that Met differently affects estimated parameters during acute and subchronic application. In the acute treatment Met mobilizes the most part of antioxidant enzymes while during the subchronic treatment these changes seems to be lost. On the contrary, the acute Met overload was not sufficient to influence on the AChE activity, while longer duration of Met loading diminished function of the enzyme. These findings point out that methionine can interfere with antioxidant defense system and cholinergic control of the heart function.
T2  - Vojnosanitetski pregled
T1  - The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue
VL  - 77
IS  - 2
SP  - 165
EP  - 173
DO  - 10.2298/VSP171213055M
ER  - 

@article{
author = "Mićović, Žarko and Kostić, Sanja and Mutavdžin, Slavica and Andrejević, Aleksa and Stamenković, Aleksandra and Čolović, Mirjana and Krstić, Danijela and Đurić, Marko and Hrnčić, Dragan and Živković, Vladimir and Jakovljević, Vladimir and Đurić, Dragan",
year = "2020",
abstract = "Background/Aim. Chronically induced hypermethioninemia leads to hyperhomocysteinemia which causes oxidative stress, atherogenesis, neurodegeneration and cancer. However, little is known about the acute and subchronic effects of DL-methionine (Met). The aim of study was to assess the effects of acutely and subchronically applied Met on oxidative stress parameters in rat plasma [enzymes: catalase (CAT), glutathione peroxidise (GPx), superoxide dismutase (SOD) and index of lipid peroxidation, malondialdehyde (MDA)], and acetylcholinesterase (AChE) activity in rat cardiac tissue. Methods. The enzymes activities, as well as MDA concentration were evaluated following acute (n = 8) and subchronic (n = 10) application of Met [i.p. 0.8 mmoL/kg body weight (b.w.) in a single dose in the acute overload or daily during three weeks in the subchronic overload]. The same was done in the control groups following application of physiological solution [i.p. 1 mL 0.9% NaCl (n = 8) in the acute overload and 0.1–0.2 mL 0.9% NaCl, daily during three weeks (n =10) in the subchronic overload]. Tested parameters were evaluated 60 minutes after application in acute experiments and after three weeks of treatment in subchronic experiments. Results. There were no difference in homocysteine values between the groups treated with Met for three weeks and the control group. Met administration significantly increased the activity of CAT and GPx after 1 h compared to the control group (p = 0.008 for both enzymes), whereas the activity of SOD and MDA concentrations were unchanged. Subchronically applied Met did not affect activity of antioxidant enzymes and MDA level. AChE activity did not show any change in rat cardiac tissue after 1 h, but it was significantly decreased after the subchronic treatment (p = 0.041). Conclusion. Results of present research indicate that Met differently affects estimated parameters during acute and subchronic application. In the acute treatment Met mobilizes the most part of antioxidant enzymes while during the subchronic treatment these changes seems to be lost. On the contrary, the acute Met overload was not sufficient to influence on the AChE activity, while longer duration of Met loading diminished function of the enzyme. These findings point out that methionine can interfere with antioxidant defense system and cholinergic control of the heart function.",
journal = "Vojnosanitetski pregled",
title = "The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue",
volume = "77",
number = "2",
pages = "165-173",
doi = "10.2298/VSP171213055M"
}

Mićović, Ž., Kostić, S., Mutavdžin, S., Andrejević, A., Stamenković, A., Čolović, M., Krstić, D., Đurić, M., Hrnčić, D., Živković, V., Jakovljević, V.,& Đurić, D.. (2020). The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue. in Vojnosanitetski pregled, 77(2), 165-173.
https://doi.org/10.2298/VSP171213055M

Mićović Ž, Kostić S, Mutavdžin S, Andrejević A, Stamenković A, Čolović M, Krstić D, Đurić M, Hrnčić D, Živković V, Jakovljević V, Đurić D. The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue. in Vojnosanitetski pregled. 2020;77(2):165-173.
doi:10.2298/VSP171213055M .

Mićović, Žarko, Kostić, Sanja, Mutavdžin, Slavica, Andrejević, Aleksa, Stamenković, Aleksandra, Čolović, Mirjana, Krstić, Danijela, Đurić, Marko, Hrnčić, Dragan, Živković, Vladimir, Jakovljević, Vladimir, Đurić, Dragan, "The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue" in Vojnosanitetski pregled, 77, no. 2 (2020):165-173,
https://doi.org/10.2298/VSP171213055M . .

TY  - JOUR
AU  - Đurić, Marko
AU  - Mutavdžin, Slavica
AU  - Lončar-Stojiljković, Dragana
AU  - Kostić, Sanja
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
AU  - Živković, Vladimir
AU  - Jakovljević, Vladimir
AU  - Đurić, Dragan
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12972
AB  - Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group – saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ sacrifice. AChE activity was measured in the rats’ cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+LNAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters.
T2  - Scripta Medica
T1  - The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity
VL  - 50
IS  - 3
SP  - 112
EP  - 116
DO  - 10.5937/scriptamed50-22658
ER  - 

@article{
author = "Đurić, Marko and Mutavdžin, Slavica and Lončar-Stojiljković, Dragana and Kostić, Sanja and Čolović, Mirjana and Krstić, Danijela and Živković, Vladimir and Jakovljević, Vladimir and Đurić, Dragan",
year = "2019",
abstract = "Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group – saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ sacrifice. AChE activity was measured in the rats’ cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+LNAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters.",
journal = "Scripta Medica",
title = "The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity",
volume = "50",
number = "3",
pages = "112-116",
doi = "10.5937/scriptamed50-22658"
}

Đurić, M., Mutavdžin, S., Lončar-Stojiljković, D., Kostić, S., Čolović, M., Krstić, D., Živković, V., Jakovljević, V.,& Đurić, D.. (2019). The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity. in Scripta Medica, 50(3), 112-116.
https://doi.org/10.5937/scriptamed50-22658

Đurić M, Mutavdžin S, Lončar-Stojiljković D, Kostić S, Čolović M, Krstić D, Živković V, Jakovljević V, Đurić D. The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity. in Scripta Medica. 2019;50(3):112-116.
doi:10.5937/scriptamed50-22658 .

Đurić, Marko, Mutavdžin, Slavica, Lončar-Stojiljković, Dragana, Kostić, Sanja, Čolović, Mirjana, Krstić, Danijela, Živković, Vladimir, Jakovljević, Vladimir, Đurić, Dragan, "The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity" in Scripta Medica, 50, no. 3 (2019):112-116,
https://doi.org/10.5937/scriptamed50-22658 . .

TY  - JOUR
AU  - Đurić, Marko
AU  - Kostić, Sanja
AU  - Lončar-Stojiljković, Dragana
AU  - Mutavdžin, Slavica
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
AU  - Stevanović, Predrag
AU  - Đurić, Dragan
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12973
AB  - Background: The importance of homocysteine (Hcy) is increasingly recognized in last few decades as an independent risk factor for atherosclerosis and thrombosis, but there is lack of data referring to influence of Hcy on plasma oxidative stress parameters as well as the role of gasotransmitters in these effects. Therefore, this study aim was to assess the role of gasotransmitter inhibitors in Hcy-induced effects on plasma oxidative stress in rats. Material and Methods: Study involved 96 male Wistar albino rats divided into 8 groups: 1) Control group - saline (1ml 0.9 % NaCl i.p.); 2) DL-Hcy (8 mmol/kg i.p. DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg i.p. Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) ZnPPR IX (30 mol/kg i.p. protoporphyrin IX zinc (ZnPPR IX), inhibitor of CO production); 5) DL-PAG (50 mg//kg i.p. DL-propargylglycine (DL-PAG), inhibitor of H2S production); 6) DL-Hcy+L-NAME (8 mmol/kg i.p. DL-Hcy + 10 mg/kg i.p. L-NAME); 7) DL-Hcy+ZnPPR IX (8 mmol/kg i.p. DL-Hcy + 30 mol/kg i.p. Zn PPR IX), and 8) DL-Hcy+DL-PAG (8 mmol/kg i.p. DL-Hcy + 50 mg//kg i.p. DL-PAG). In all experimental groups, tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals' euthanasia. In the collected blood samples malondialdehyde concentration, catalase, glutathione peroxidase and superoxide dismutase activity were measured. Results: Applied substances induced rapid and strong increase of plasma antioxidant enzymatic activity probably as a compensatory response to its pro-oxidant influence. Conclusion: The effects of Hcy on the activity of plasma antioxidant enzymes are in part mediated via interaction with gasotransmitters.
T2  - Scripta Medica
T1  - The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma
VL  - 50
IS  - 1
SP  - 6
EP  - 12
DO  - 10.5937/scriptamed50-21100
ER  - 

@article{
author = "Đurić, Marko and Kostić, Sanja and Lončar-Stojiljković, Dragana and Mutavdžin, Slavica and Čolović, Mirjana and Krstić, Danijela and Stevanović, Predrag and Đurić, Dragan",
year = "2019",
abstract = "Background: The importance of homocysteine (Hcy) is increasingly recognized in last few decades as an independent risk factor for atherosclerosis and thrombosis, but there is lack of data referring to influence of Hcy on plasma oxidative stress parameters as well as the role of gasotransmitters in these effects. Therefore, this study aim was to assess the role of gasotransmitter inhibitors in Hcy-induced effects on plasma oxidative stress in rats. Material and Methods: Study involved 96 male Wistar albino rats divided into 8 groups: 1) Control group - saline (1ml 0.9 % NaCl i.p.); 2) DL-Hcy (8 mmol/kg i.p. DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg i.p. Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) ZnPPR IX (30 mol/kg i.p. protoporphyrin IX zinc (ZnPPR IX), inhibitor of CO production); 5) DL-PAG (50 mg//kg i.p. DL-propargylglycine (DL-PAG), inhibitor of H2S production); 6) DL-Hcy+L-NAME (8 mmol/kg i.p. DL-Hcy + 10 mg/kg i.p. L-NAME); 7) DL-Hcy+ZnPPR IX (8 mmol/kg i.p. DL-Hcy + 30 mol/kg i.p. Zn PPR IX), and 8) DL-Hcy+DL-PAG (8 mmol/kg i.p. DL-Hcy + 50 mg//kg i.p. DL-PAG). In all experimental groups, tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals' euthanasia. In the collected blood samples malondialdehyde concentration, catalase, glutathione peroxidase and superoxide dismutase activity were measured. Results: Applied substances induced rapid and strong increase of plasma antioxidant enzymatic activity probably as a compensatory response to its pro-oxidant influence. Conclusion: The effects of Hcy on the activity of plasma antioxidant enzymes are in part mediated via interaction with gasotransmitters.",
journal = "Scripta Medica",
title = "The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma",
volume = "50",
number = "1",
pages = "6-12",
doi = "10.5937/scriptamed50-21100"
}

Đurić, M., Kostić, S., Lončar-Stojiljković, D., Mutavdžin, S., Čolović, M., Krstić, D., Stevanović, P.,& Đurić, D.. (2019). The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma. in Scripta Medica, 50(1), 6-12.
https://doi.org/10.5937/scriptamed50-21100

Đurić M, Kostić S, Lončar-Stojiljković D, Mutavdžin S, Čolović M, Krstić D, Stevanović P, Đurić D. The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma. in Scripta Medica. 2019;50(1):6-12.
doi:10.5937/scriptamed50-21100 .

Đurić, Marko, Kostić, Sanja, Lončar-Stojiljković, Dragana, Mutavdžin, Slavica, Čolović, Mirjana, Krstić, Danijela, Stevanović, Predrag, Đurić, Dragan, "The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma" in Scripta Medica, 50, no. 1 (2019):6-12,
https://doi.org/10.5937/scriptamed50-21100 . .

TY  - JOUR
AU  - Kornjača, Duško
AU  - Živković, Vladimir I.
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Marko
AU  - Stanković, Sanja
AU  - Mutavdžin, Slavica
AU  - Jakovljević, Vladimir Lj.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641700041K
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7714
AB  - The aim of this study was to assess the effects of DL-homocysteine (DL-Hcy) and DL-homocysteine thiolactone (DL-Hcy TLHC) on selected serum biochemical parameters, markers of oxidative stress and the activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) in the plasma, as well as on acetylcholinesterase (AChE) activity in the cardiac tissue homogenate in the rat. Male Wistar rats were divided into three groups as follows: control group (1 mL 0.9% NaCl, intraperitoneal (i.p.) injection), DL-Hcy group (8 mmol/kg body mass (b.m.), i.p.) or DL-Hcy TLHC group (8 mmol/kg b.m., i.p.). One hour after administration, the rats were euthanized, whole blood was collected for biochemical analysis, and the heart was excised. Following the i.p. administration of DL-Hcy and DL-Hcy TLHC, the activities of antioxidant enzymes were mostly significantly increased, while plasma malondialdehyde (MDA) was decreased. Administration of DL-Hcy and DL-Hcy TLHC significantly inhibited AChE activity in rat cardiac tissue. Our findings suggest that DL-Hcy and DL-Hcy TLHC exerted prooxidant effects; however, the decrease in MDA points to an inverse response to the increase in antioxidant enzyme activities. While both substances inhibited AChE activity in rat cardiac tissue, DL-Hcy TLHC induced stronger effects than DL-Hcy.
T2  - Archives of Biological Sciences
T1  - The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat
VL  - 70
IS  - 2
SP  - 241
EP  - 248
DO  - 10.2298/ABS170731041K
ER  - 

@article{
author = "Kornjača, Duško and Živković, Vladimir I. and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Marko and Stanković, Sanja and Mutavdžin, Slavica and Jakovljević, Vladimir Lj. and Đurić, Dragan M.",
year = "2018",
abstract = "The aim of this study was to assess the effects of DL-homocysteine (DL-Hcy) and DL-homocysteine thiolactone (DL-Hcy TLHC) on selected serum biochemical parameters, markers of oxidative stress and the activities of antioxidant enzymes (catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD)) in the plasma, as well as on acetylcholinesterase (AChE) activity in the cardiac tissue homogenate in the rat. Male Wistar rats were divided into three groups as follows: control group (1 mL 0.9% NaCl, intraperitoneal (i.p.) injection), DL-Hcy group (8 mmol/kg body mass (b.m.), i.p.) or DL-Hcy TLHC group (8 mmol/kg b.m., i.p.). One hour after administration, the rats were euthanized, whole blood was collected for biochemical analysis, and the heart was excised. Following the i.p. administration of DL-Hcy and DL-Hcy TLHC, the activities of antioxidant enzymes were mostly significantly increased, while plasma malondialdehyde (MDA) was decreased. Administration of DL-Hcy and DL-Hcy TLHC significantly inhibited AChE activity in rat cardiac tissue. Our findings suggest that DL-Hcy and DL-Hcy TLHC exerted prooxidant effects; however, the decrease in MDA points to an inverse response to the increase in antioxidant enzyme activities. While both substances inhibited AChE activity in rat cardiac tissue, DL-Hcy TLHC induced stronger effects than DL-Hcy.",
journal = "Archives of Biological Sciences",
title = "The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat",
volume = "70",
number = "2",
pages = "241-248",
doi = "10.2298/ABS170731041K"
}

Kornjača, D., Živković, V. I., Krstić, D. Z., Čolović, M. B., Đurić, M., Stanković, S., Mutavdžin, S., Jakovljević, V. Lj.,& Đurić, D. M.. (2018). The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat. in Archives of Biological Sciences, 70(2), 241-248.
https://doi.org/10.2298/ABS170731041K

Kornjača D, Živković VI, Krstić DZ, Čolović MB, Đurić M, Stanković S, Mutavdžin S, Jakovljević VL, Đurić DM. The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat. in Archives of Biological Sciences. 2018;70(2):241-248.
doi:10.2298/ABS170731041K .

Kornjača, Duško, Živković, Vladimir I., Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Marko, Stanković, Sanja, Mutavdžin, Slavica, Jakovljević, Vladimir Lj., Đurić, Dragan M., "The effects of acute hyperhomocysteinemia induced by DL-homocysteine or DL-homocysteine thiolactone on serum biochemical parameters, plasma antioxidant enzyme and cardiac acetylcholinesterase activities in the rat" in Archives of Biological Sciences, 70, no. 2 (2018):241-248,
https://doi.org/10.2298/ABS170731041K . .

TY  - JOUR
AU  - Jakovljević-Uzelac, Jovana
AU  - Stanić, Marina
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s11010-017-3238-z
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7788
AB  - The objective of this study was to investigate in vitro effects of 10 A mu M dl-homocysteine (dl-Hcy), dl-homocysteine thiolactone-hydrochloride (dl-Hcy TLHC), and l-homocysteine thiolactone-hydrochloride (l-Hcy TLHC) on the oxygen consumption of rat heart tissue homogenate, as well as the involvement of the gasotransmitters NO, H2S and CO in the effects of the most toxic homocysteine compound, dl-Hcy TLHC. The possible contribution of the gasotransmitters in these effects was estimated by using the appropriate inhibitors of their synthesis (N (omega)-nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (dl-PAG), and zinc protoporphyrin IX (ZnPPR IX), respectively). The oxygen consumption of rat heart tissue homogenate was measured by Clark/type oxygen electrode in the absence and presence of the investigated compounds. All three homocysteine-based compounds caused a similar decrease in the oxygen consumption rate compared to control: 15.19 +/- 4.01%, 12.42 +/- 1.01%, and 16.43 +/- 4.52% for dl-Hcy, dl-Hcy TLHC, or l-Hcy TLHC, respectively. All applied inhibitors of gasotransmitter synthesis also decreased the oxygen consumption rate of tissue homogenate related to control: 13.53 +/- 1.35% for l-NAME (30 A mu M), 5.32 +/- 1.23% for dl-PAG (10 A mu M), and 5.56 +/- 1.39% for ZnPPR IX (10 A mu M). Simultaneous effect of l-NAME (30 A mu M) or ZnPPR IX (10 A mu M) with dl-Hcy TLHC (10 A mu M) caused a larger decrease of oxygen consumption compared to each of the substances individually. However, when dl-PAG (10 A mu M) was applied together with dl-Hcy TLHC (10 A mu M), it attenuated the effect of dl-Hcy TLHC from 12.42 +/- 1.01 to 9.22 +/- 1.58%. In conclusion, cardiotoxicity induced by Hcy-related compounds, which was shown in our previous research, could result from the inhibition of the oxygen consumption, and might be mediated by the certain gasotransmitters.
T2  - Molecular and Cellular Biochemistry
T1  - Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters
VL  - 444
IS  - 1-2
SP  - 143
EP  - 148
DO  - 10.1007/s11010-017-3238-z
ER  - 

@article{
author = "Jakovljević-Uzelac, Jovana and Stanić, Marina and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Dragan M.",
year = "2018",
abstract = "The objective of this study was to investigate in vitro effects of 10 A mu M dl-homocysteine (dl-Hcy), dl-homocysteine thiolactone-hydrochloride (dl-Hcy TLHC), and l-homocysteine thiolactone-hydrochloride (l-Hcy TLHC) on the oxygen consumption of rat heart tissue homogenate, as well as the involvement of the gasotransmitters NO, H2S and CO in the effects of the most toxic homocysteine compound, dl-Hcy TLHC. The possible contribution of the gasotransmitters in these effects was estimated by using the appropriate inhibitors of their synthesis (N (omega)-nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (dl-PAG), and zinc protoporphyrin IX (ZnPPR IX), respectively). The oxygen consumption of rat heart tissue homogenate was measured by Clark/type oxygen electrode in the absence and presence of the investigated compounds. All three homocysteine-based compounds caused a similar decrease in the oxygen consumption rate compared to control: 15.19 +/- 4.01%, 12.42 +/- 1.01%, and 16.43 +/- 4.52% for dl-Hcy, dl-Hcy TLHC, or l-Hcy TLHC, respectively. All applied inhibitors of gasotransmitter synthesis also decreased the oxygen consumption rate of tissue homogenate related to control: 13.53 +/- 1.35% for l-NAME (30 A mu M), 5.32 +/- 1.23% for dl-PAG (10 A mu M), and 5.56 +/- 1.39% for ZnPPR IX (10 A mu M). Simultaneous effect of l-NAME (30 A mu M) or ZnPPR IX (10 A mu M) with dl-Hcy TLHC (10 A mu M) caused a larger decrease of oxygen consumption compared to each of the substances individually. However, when dl-PAG (10 A mu M) was applied together with dl-Hcy TLHC (10 A mu M), it attenuated the effect of dl-Hcy TLHC from 12.42 +/- 1.01 to 9.22 +/- 1.58%. In conclusion, cardiotoxicity induced by Hcy-related compounds, which was shown in our previous research, could result from the inhibition of the oxygen consumption, and might be mediated by the certain gasotransmitters.",
journal = "Molecular and Cellular Biochemistry",
title = "Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters",
volume = "444",
number = "1-2",
pages = "143-148",
doi = "10.1007/s11010-017-3238-z"
}

Jakovljević-Uzelac, J., Stanić, M., Krstić, D. Z., Čolović, M. B.,& Đurić, D. M.. (2018). Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters. in Molecular and Cellular Biochemistry, 444(1-2), 143-148.
https://doi.org/10.1007/s11010-017-3238-z

Jakovljević-Uzelac J, Stanić M, Krstić DZ, Čolović MB, Đurić DM. Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters. in Molecular and Cellular Biochemistry. 2018;444(1-2):143-148.
doi:10.1007/s11010-017-3238-z .

Jakovljević-Uzelac, Jovana, Stanić, Marina, Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Dragan M., "Effects of homocysteine and its related compounds on oxygen consumption of the rat heart tissue homogenate: the role of different gasotransmitters" in Molecular and Cellular Biochemistry, 444, no. 1-2 (2018):143-148,
https://doi.org/10.1007/s11010-017-3238-z . .

TY  - JOUR
AU  - Stojanović, Marija
AU  - Todorović, Dajana D.
AU  - Šćepanović, Ljiljana
AU  - Mitrović, Dušan M.
AU  - Borozan, Sunčica Z.
AU  - Dragutinović, Vesna V.
AU  - Labudović-Borović, Milica
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Đurić, Dragan M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s11010-018-3311-2
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8060
AB  - The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.
T2  - Molecular and Cellular Biochemistry
T1  - Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue
VL  - 448
IS  - 1-2
SP  - 43
EP  - 50
DO  - 10.1007/s11010-018-3311-2
ER  - 

@article{
author = "Stojanović, Marija and Todorović, Dajana D. and Šćepanović, Ljiljana and Mitrović, Dušan M. and Borozan, Sunčica Z. and Dragutinović, Vesna V. and Labudović-Borović, Milica and Krstić, Danijela Z. and Čolović, Mirjana B. and Đurić, Dragan M.",
year = "2018",
abstract = "The aim of this study was to assess the effects of l-cysteine (Cys) (7 mg/kg) and N-acetyl-l-cysteine (NAC) (50 mg/kg) in the rat liver caused by subchronic i.p. application of methionine (Met) (0.8 mmol/kg) during 21 days. Malondialdehyde (MDA) concentration, glutathione content (GSH), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and acetylcholinesterase (AchE) activities were determined in the liver tissue and activities of liver enzymes (AST, ALT, ALP, and GGT) and concentrations of total proteins and albumin were determinated in plasma/serum. Catalase, superoxide dismutase, and acetylcholinesterase activities were increased by Cys and NAC. Met caused periportal mononuclear infiltration and rare focal necrosis of hepatocytes. In Cys- and NAC-supplemented groups, intracellular edema and microvesicular fatty changes without necrosis were noticed. We observed decrease of AST, ALT, and ALP activity in the methionine-treated group. Our results indicate that Cys and NAC application can increase activity of antioxidative enzymes and prevent intensive histological changes in liver in condition of subchronic methionine exposure.",
journal = "Molecular and Cellular Biochemistry",
title = "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue",
volume = "448",
number = "1-2",
pages = "43-50",
doi = "10.1007/s11010-018-3311-2"
}

Stojanović, M., Todorović, D. D., Šćepanović, L., Mitrović, D. M., Borozan, S. Z., Dragutinović, V. V., Labudović-Borović, M., Krstić, D. Z., Čolović, M. B.,& Đurić, D. M.. (2018). Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry, 448(1-2), 43-50.
https://doi.org/10.1007/s11010-018-3311-2

Stojanović M, Todorović DD, Šćepanović L, Mitrović DM, Borozan SZ, Dragutinović VV, Labudović-Borović M, Krstić DZ, Čolović MB, Đurić DM. Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue. in Molecular and Cellular Biochemistry. 2018;448(1-2):43-50.
doi:10.1007/s11010-018-3311-2 .

Stojanović, Marija, Todorović, Dajana D., Šćepanović, Ljiljana, Mitrović, Dušan M., Borozan, Sunčica Z., Dragutinović, Vesna V., Labudović-Borović, Milica, Krstić, Danijela Z., Čolović, Mirjana B., Đurić, Dragan M., "Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue" in Molecular and Cellular Biochemistry, 448, no. 1-2 (2018):43-50,
https://doi.org/10.1007/s11010-018-3311-2 . .

TY  - JOUR
AU  - Stojanović, Marija
AU  - Scepanovic, Ljiljana
AU  - Bosnic, Olivera
AU  - Mitrovic, Dusan
AU  - Jozanov-Stankov, Olga
AU  - Scepanovic, Vuk
AU  - Scepanovic, Radomir
AU  - Stojanovic, Teja
AU  - Ilic, Slobodan
AU  - Đurić, Dragan M.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1117
AB  - Oxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chrons disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hour incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 mu mol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1: 10). Homogenates were centrifuged at 10000 for 10 min at 4 degrees C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p LT 0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p LT 0.001. Exposition to high D, L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p LT 0.01). Homocysteine also modified the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p LT 0.001). Homocysteine induced a consistent oxidative stress in rats intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine.
T2  - Acta Veterinaria, Beograd
T1  - Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver
VL  - 66
IS  - 1
SP  - 26
EP  - 36
DO  - 10.1515/acve-2016-0002
ER  - 

@article{
author = "Stojanović, Marija and Scepanovic, Ljiljana and Bosnic, Olivera and Mitrovic, Dusan and Jozanov-Stankov, Olga and Scepanovic, Vuk and Scepanovic, Radomir and Stojanovic, Teja and Ilic, Slobodan and Đurić, Dragan M.",
year = "2016",
abstract = "Oxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chrons disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hour incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 mu mol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1: 10). Homogenates were centrifuged at 10000 for 10 min at 4 degrees C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p LT 0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p LT 0.001. Exposition to high D, L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p LT 0.01). Homocysteine also modified the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p LT 0.001). Homocysteine induced a consistent oxidative stress in rats intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine.",
journal = "Acta Veterinaria, Beograd",
title = "Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver",
volume = "66",
number = "1",
pages = "26-36",
doi = "10.1515/acve-2016-0002"
}

Stojanović, M., Scepanovic, L., Bosnic, O., Mitrovic, D., Jozanov-Stankov, O., Scepanovic, V., Scepanovic, R., Stojanovic, T., Ilic, S.,& Đurić, D. M.. (2016). Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver. in Acta Veterinaria, Beograd, 66(1), 26-36.
https://doi.org/10.1515/acve-2016-0002

Stojanović M, Scepanovic L, Bosnic O, Mitrovic D, Jozanov-Stankov O, Scepanovic V, Scepanovic R, Stojanovic T, Ilic S, Đurić DM. Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver. in Acta Veterinaria, Beograd. 2016;66(1):26-36.
doi:10.1515/acve-2016-0002 .

Stojanović, Marija, Scepanovic, Ljiljana, Bosnic, Olivera, Mitrovic, Dusan, Jozanov-Stankov, Olga, Scepanovic, Vuk, Scepanovic, Radomir, Stojanovic, Teja, Ilic, Slobodan, Đurić, Dragan M., "Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver" in Acta Veterinaria, Beograd, 66, no. 1 (2016):26-36,
https://doi.org/10.1515/acve-2016-0002 . .