TY  - CONF
AU  - Mitić, Miloš
AU  - Simić, Iva
AU  - Đorđević, Ana D.
AU  - Đorđević, J.
AU  - Radojčić, Marija
AU  - Adžić, Miroslav
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9320
AB  - Dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis has been
implicated in the pathophysiology of depression and stress disorders.
Glucocorticoids, key regulators of stress response, have diverse effects on cellular
processes in the hippocampus. Beside non genomic pathways, glucocorticoids
effects are mediated through activation of the glucocorticoid receptor (GR), a
ligand activated transcriptional factor that belongs to the nuclear hormone receptor
superfamily. We analysed the GR protein level both, in the cytoplasmic and
nuclear compartments in Wistar rat hippocampus, exposed to 3 week social
isolation stress upon chronic fluoxetine treatment. Under chronic stress,
corticosterone level was decreased compared to the control and treatment with
fluoxetine did not change its level significantly in stressed animals. At the
molecular level, fluoxetine significantly decreased the level of nuclear GR protein
in the brain hippocampus of the chronically stressed rats. Fluoxetine reversed the
nuclear level of GR disrupted by chronic psychosocial isolation (CPSI), but it
failed to normalize HPA axis activity.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Fluoxetine decreases the level of nuclear glucocorticoid receptor in wistar rat hippocampus under chronic stress
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9320
ER  - 

@conference{
author = "Mitić, Miloš and Simić, Iva and Đorđević, Ana D. and Đorđević, J. and Radojčić, Marija and Adžić, Miroslav",
year = "2010",
abstract = "Dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis has been
implicated in the pathophysiology of depression and stress disorders.
Glucocorticoids, key regulators of stress response, have diverse effects on cellular
processes in the hippocampus. Beside non genomic pathways, glucocorticoids
effects are mediated through activation of the glucocorticoid receptor (GR), a
ligand activated transcriptional factor that belongs to the nuclear hormone receptor
superfamily. We analysed the GR protein level both, in the cytoplasmic and
nuclear compartments in Wistar rat hippocampus, exposed to 3 week social
isolation stress upon chronic fluoxetine treatment. Under chronic stress,
corticosterone level was decreased compared to the control and treatment with
fluoxetine did not change its level significantly in stressed animals. At the
molecular level, fluoxetine significantly decreased the level of nuclear GR protein
in the brain hippocampus of the chronically stressed rats. Fluoxetine reversed the
nuclear level of GR disrupted by chronic psychosocial isolation (CPSI), but it
failed to normalize HPA axis activity.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Fluoxetine decreases the level of nuclear glucocorticoid receptor in wistar rat hippocampus under chronic stress",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9320"
}

Mitić, M., Simić, I., Đorđević, A. D., Đorđević, J., Radojčić, M.,& Adžić, M.. (2010). Fluoxetine decreases the level of nuclear glucocorticoid receptor in wistar rat hippocampus under chronic stress. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
https://hdl.handle.net/21.15107/rcub_vinar_9320

Mitić M, Simić I, Đorđević AD, Đorđević J, Radojčić M, Adžić M. Fluoxetine decreases the level of nuclear glucocorticoid receptor in wistar rat hippocampus under chronic stress. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;.
https://hdl.handle.net/21.15107/rcub_vinar_9320 .

Mitić, Miloš, Simić, Iva, Đorđević, Ana D., Đorđević, J., Radojčić, Marija, Adžić, Miroslav, "Fluoxetine decreases the level of nuclear glucocorticoid receptor in wistar rat hippocampus under chronic stress" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010),
https://hdl.handle.net/21.15107/rcub_vinar_9320 .

TY  - JOUR
AU  - Đorđević, Jelena D.
AU  - Đorđević, Ana D.
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Radojčić, Marija
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4153
AB  - Clinical reports suggest close interactions between stressors, particularly those of long duration, and liver diseases, such as hepatic inflammation, that is proposed to occur via reactive oxygen species. In the present study we have used 21-day social isolation of male Wistar rats as a model of chronic stress to investigate protein expression/activity of liver antioxidant enzymes: superoxide dismutases (SODs), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GLR), and protein expression of their upstream regulators: glucocorticoid receptor (GR) and nuclear factor kappa B (NFkB). We have also characterized these parameters in either naive or chronically stressed animals that were challenged by 30-min acute immobilization. We found that chronic isolation caused decrease in serum corticosterone (CORT) and blood glucose (GLU), increase in NFkB signaling, and disproportion between CuZnSOD, peroxidases (CAT, GPx) and GLR, thus promoting H2O2 accumulation and prooxidative state in liver. The overall results suggested that chronic stress exaggerated responsiveness to subsequent stressor at the level of CORT and GLU, and potentiated GLR response, but compromised the restoration of oxido-reductive balance due to irreversible alterations in MnSOD and GPx.
T2  - Physiological Research
T1  - Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats
VL  - 59
IS  - 5
SP  - 729
EP  - 736
UR  - https://hdl.handle.net/21.15107/rcub_vinar_4153
ER  - 

@article{
author = "Đorđević, Jelena D. and Đorđević, Ana D. and Adžić, Miroslav and Nićiforović, Ana and Radojčić, Marija",
year = "2010",
abstract = "Clinical reports suggest close interactions between stressors, particularly those of long duration, and liver diseases, such as hepatic inflammation, that is proposed to occur via reactive oxygen species. In the present study we have used 21-day social isolation of male Wistar rats as a model of chronic stress to investigate protein expression/activity of liver antioxidant enzymes: superoxide dismutases (SODs), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GLR), and protein expression of their upstream regulators: glucocorticoid receptor (GR) and nuclear factor kappa B (NFkB). We have also characterized these parameters in either naive or chronically stressed animals that were challenged by 30-min acute immobilization. We found that chronic isolation caused decrease in serum corticosterone (CORT) and blood glucose (GLU), increase in NFkB signaling, and disproportion between CuZnSOD, peroxidases (CAT, GPx) and GLR, thus promoting H2O2 accumulation and prooxidative state in liver. The overall results suggested that chronic stress exaggerated responsiveness to subsequent stressor at the level of CORT and GLU, and potentiated GLR response, but compromised the restoration of oxido-reductive balance due to irreversible alterations in MnSOD and GPx.",
journal = "Physiological Research",
title = "Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats",
volume = "59",
number = "5",
pages = "729-736",
url = "https://hdl.handle.net/21.15107/rcub_vinar_4153"
}

Đorđević, J. D., Đorđević, A. D., Adžić, M., Nićiforović, A.,& Radojčić, M.. (2010). Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats. in Physiological Research, 59(5), 729-736.
https://hdl.handle.net/21.15107/rcub_vinar_4153

Đorđević JD, Đorđević AD, Adžić M, Nićiforović A, Radojčić M. Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats. in Physiological Research. 2010;59(5):729-736.
https://hdl.handle.net/21.15107/rcub_vinar_4153 .

Đorđević, Jelena D., Đorđević, Ana D., Adžić, Miroslav, Nićiforović, Ana, Radojčić, Marija, "Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats" in Physiological Research, 59, no. 5 (2010):729-736,
https://hdl.handle.net/21.15107/rcub_vinar_4153 .

TY  - JOUR
AU  - Đorđević, Ana D.
AU  - Adžić, Miroslav
AU  - Đorđević, Jelena D.
AU  - Radojčić, Marija
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4060
AB  - Successful adaptation to stress involves synergized actions of glucocorticoids and catecholamines at several levels of the CNS, including the prefrontal cortex (PFC). Inside the PFC, hormonal signals trigger concerted actions of transcriptional factors, such as glucocorticoid receptor (GR) and nuclear factor kappa B (NF kappa B), culminating in a balanced, proadaptive expression of their common genes, such as proplastic NCAM and/or apoptotic Bax and Bcl-2. In the present study, we hypothesized that chronic stress may compromise the balance between GR and NF kappa B signals and lead to an altered/maladaptive expression of their cognate genes in the PFC. Our results obtained with Wistar rats exposed to chronic social isolation indicated alterations of the GR relative to the NF kappa B, in favor of the GA, in both the cytoplasmic and the nuclear compartments of the PFC. Although these alterations did not affect the induction of proplastic NCAM gene, they decreased the NCAM sialylation necessary for plastic response and caused marked relocation of the mitochondrial membrane antiapoptotic Bcl-2 protein to its cytoplasmic form. Moreover, the compromised PSA-NCAM plastic response found under chronic stress was sustained after exposure of animals to the subsequent acute stress, whereas the proapoptotic signals were further emphasized. It is concluded that chronic social isolation of Wistar animals leads to a maladaptive response of the PFC, considering the diminishment of its plastic potential and potentiating of apoptosis. Such conditions in the PFC are likely to compromise its ability to interact with other CNS structures, such as the hippocampus, which is necessary for successful adaptation to stress. (C) 2010 Wiley-Liss, Inc.
T2  - Journal of Neuroscience Research
T1  - Chronic Social Isolation Suppresses Proplastic Response and Promotes Proapoptotic Signalling in Prefrontal Cortex of Wistar Rats
VL  - 88
IS  - 11
SP  - 2524
EP  - 2533
DO  - 10.1002/jnr.22403
ER  - 

@article{
author = "Đorđević, Ana D. and Adžić, Miroslav and Đorđević, Jelena D. and Radojčić, Marija",
year = "2010",
abstract = "Successful adaptation to stress involves synergized actions of glucocorticoids and catecholamines at several levels of the CNS, including the prefrontal cortex (PFC). Inside the PFC, hormonal signals trigger concerted actions of transcriptional factors, such as glucocorticoid receptor (GR) and nuclear factor kappa B (NF kappa B), culminating in a balanced, proadaptive expression of their common genes, such as proplastic NCAM and/or apoptotic Bax and Bcl-2. In the present study, we hypothesized that chronic stress may compromise the balance between GR and NF kappa B signals and lead to an altered/maladaptive expression of their cognate genes in the PFC. Our results obtained with Wistar rats exposed to chronic social isolation indicated alterations of the GR relative to the NF kappa B, in favor of the GA, in both the cytoplasmic and the nuclear compartments of the PFC. Although these alterations did not affect the induction of proplastic NCAM gene, they decreased the NCAM sialylation necessary for plastic response and caused marked relocation of the mitochondrial membrane antiapoptotic Bcl-2 protein to its cytoplasmic form. Moreover, the compromised PSA-NCAM plastic response found under chronic stress was sustained after exposure of animals to the subsequent acute stress, whereas the proapoptotic signals were further emphasized. It is concluded that chronic social isolation of Wistar animals leads to a maladaptive response of the PFC, considering the diminishment of its plastic potential and potentiating of apoptosis. Such conditions in the PFC are likely to compromise its ability to interact with other CNS structures, such as the hippocampus, which is necessary for successful adaptation to stress. (C) 2010 Wiley-Liss, Inc.",
journal = "Journal of Neuroscience Research",
title = "Chronic Social Isolation Suppresses Proplastic Response and Promotes Proapoptotic Signalling in Prefrontal Cortex of Wistar Rats",
volume = "88",
number = "11",
pages = "2524-2533",
doi = "10.1002/jnr.22403"
}

Đorđević, A. D., Adžić, M., Đorđević, J. D.,& Radojčić, M.. (2010). Chronic Social Isolation Suppresses Proplastic Response and Promotes Proapoptotic Signalling in Prefrontal Cortex of Wistar Rats. in Journal of Neuroscience Research, 88(11), 2524-2533.
https://doi.org/10.1002/jnr.22403

Đorđević AD, Adžić M, Đorđević JD, Radojčić M. Chronic Social Isolation Suppresses Proplastic Response and Promotes Proapoptotic Signalling in Prefrontal Cortex of Wistar Rats. in Journal of Neuroscience Research. 2010;88(11):2524-2533.
doi:10.1002/jnr.22403 .

Đorđević, Ana D., Adžić, Miroslav, Đorđević, Jelena D., Radojčić, Marija, "Chronic Social Isolation Suppresses Proplastic Response and Promotes Proapoptotic Signalling in Prefrontal Cortex of Wistar Rats" in Journal of Neuroscience Research, 88, no. 11 (2010):2524-2533,
https://doi.org/10.1002/jnr.22403 . .

TY  - JOUR
AU  - Đorđević, Jelena D.
AU  - Đorđević, Ana D.
AU  - Adžić, Miroslav
AU  - Radoičić, Marija
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4020
AB  - Chronic neuroendocrine stress usually leads to the elevation of the stress hormones and increased metabolic rate, which is frequently accompanied by oxidative damage to the CNS. In the present study we hypothesized that chronic psychosocial isolation (CPSI) of male Wistar rats, characterized by decreased serum corticosterone (CORT), unaltered catecholamines (CTs), and low blood glucose (GLU), may also promote oxidative imbalance in the CNS, by targeting antioxidant defense system. To test it, we have examined the relation between these input signals and protein expression/activity of antioxidant enzymes (AOEs): superoxide dismutases (SODs), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GLR) in the hippocampus (HIPPO) of CPSI animals. We found that CPSI did not affect SODs or CAT, but decreased activity of GPx and compromised GLR, an enzyme highly dependent on blood GLU for its substrate precursor. Further, we have tested whether the CPSI experience altered AOEs response to a novelty stress, and found that it attenuated peroxide-metabolizing enzymes, CAT and GPx, and decreased GLR activity, even though blood GLU was restored. The altered ratios of hippocampal AOEs in CPSI animals, which were worsened under the combined stress conditions, may lead to the accumulation of peroxide products and oxidative imbalance. The mechanism by which CPSI generate oxidative imbalance in the HIPPO is most likely based on poor systemic energy conditions set by this stress. Such conditions may cause functional decline of CNS structures, such as HIPPO, and are likely to promote state linked to onset of many mood disorders.
T2  - Cellular and Molecular Neurobiology
T1  - Chronic Social Isolation Compromises the Activity of Both Glutathione Peroxidase and Catalase in Hippocampus of Male Wistar Rats
VL  - 30
IS  - 5
SP  - 693
EP  - 700
DO  - 10.1007/s10571-009-9493-0
ER  - 

@article{
author = "Đorđević, Jelena D. and Đorđević, Ana D. and Adžić, Miroslav and Radoičić, Marija",
year = "2010",
abstract = "Chronic neuroendocrine stress usually leads to the elevation of the stress hormones and increased metabolic rate, which is frequently accompanied by oxidative damage to the CNS. In the present study we hypothesized that chronic psychosocial isolation (CPSI) of male Wistar rats, characterized by decreased serum corticosterone (CORT), unaltered catecholamines (CTs), and low blood glucose (GLU), may also promote oxidative imbalance in the CNS, by targeting antioxidant defense system. To test it, we have examined the relation between these input signals and protein expression/activity of antioxidant enzymes (AOEs): superoxide dismutases (SODs), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GLR) in the hippocampus (HIPPO) of CPSI animals. We found that CPSI did not affect SODs or CAT, but decreased activity of GPx and compromised GLR, an enzyme highly dependent on blood GLU for its substrate precursor. Further, we have tested whether the CPSI experience altered AOEs response to a novelty stress, and found that it attenuated peroxide-metabolizing enzymes, CAT and GPx, and decreased GLR activity, even though blood GLU was restored. The altered ratios of hippocampal AOEs in CPSI animals, which were worsened under the combined stress conditions, may lead to the accumulation of peroxide products and oxidative imbalance. The mechanism by which CPSI generate oxidative imbalance in the HIPPO is most likely based on poor systemic energy conditions set by this stress. Such conditions may cause functional decline of CNS structures, such as HIPPO, and are likely to promote state linked to onset of many mood disorders.",
journal = "Cellular and Molecular Neurobiology",
title = "Chronic Social Isolation Compromises the Activity of Both Glutathione Peroxidase and Catalase in Hippocampus of Male Wistar Rats",
volume = "30",
number = "5",
pages = "693-700",
doi = "10.1007/s10571-009-9493-0"
}

Đorđević, J. D., Đorđević, A. D., Adžić, M.,& Radoičić, M.. (2010). Chronic Social Isolation Compromises the Activity of Both Glutathione Peroxidase and Catalase in Hippocampus of Male Wistar Rats. in Cellular and Molecular Neurobiology, 30(5), 693-700.
https://doi.org/10.1007/s10571-009-9493-0

Đorđević JD, Đorđević AD, Adžić M, Radoičić M. Chronic Social Isolation Compromises the Activity of Both Glutathione Peroxidase and Catalase in Hippocampus of Male Wistar Rats. in Cellular and Molecular Neurobiology. 2010;30(5):693-700.
doi:10.1007/s10571-009-9493-0 .

Đorđević, Jelena D., Đorđević, Ana D., Adžić, Miroslav, Radoičić, Marija, "Chronic Social Isolation Compromises the Activity of Both Glutathione Peroxidase and Catalase in Hippocampus of Male Wistar Rats" in Cellular and Molecular Neurobiology, 30, no. 5 (2010):693-700,
https://doi.org/10.1007/s10571-009-9493-0 . .

TY  - JOUR
AU  - Đorđević, Jelena D.
AU  - Nićiforović, Ana
AU  - Radojčić, Marija
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6816
AB  - Altered activities of antioxidant defence system enzymes and the levels of free radicals scavengers have been found to correlate with various physiological or pathological conditions, including stress. The aim of this study was to determine the effects of chronic 21 day isolation stress on antioxidant enzymes (AOEs) expression and activity in Wistar rat liver tissue. The serum corticosterone (CORT) and glucose (GLU) levels were also measured, as one of the most important indicators of stress. Our data revealed that in chronic stress conditions, when both CORT and GLU were low, the AOEs expression was markedly induced. This increase in MnSOD, CuZnSOD, and catalase exhibited similar trend implying efficient detoxification of O(2) radical anion and H(2)O(2). However, this trend was not followed by the respective enzyme activity. While the total SOD activity was induced by the stress, catalase activity remained unaltered. This discrepancy led us to a conclusion that chronic isolation stress may cause oxidant-antioxidant imbalance in rat liver tissue, favoring H(2)O(2) accumulation.
T2  - Russian Journal of Physical Chemistry A
T1  - Antioxidant Enzymes Expression and Activity in Liver of Stressed Wistar Rat
VL  - 83
IS  - 9
SP  - 1617
EP  - 1620
DO  - 10.1134/S0036024409090362
ER  - 

@article{
author = "Đorđević, Jelena D. and Nićiforović, Ana and Radojčić, Marija",
year = "2009",
abstract = "Altered activities of antioxidant defence system enzymes and the levels of free radicals scavengers have been found to correlate with various physiological or pathological conditions, including stress. The aim of this study was to determine the effects of chronic 21 day isolation stress on antioxidant enzymes (AOEs) expression and activity in Wistar rat liver tissue. The serum corticosterone (CORT) and glucose (GLU) levels were also measured, as one of the most important indicators of stress. Our data revealed that in chronic stress conditions, when both CORT and GLU were low, the AOEs expression was markedly induced. This increase in MnSOD, CuZnSOD, and catalase exhibited similar trend implying efficient detoxification of O(2) radical anion and H(2)O(2). However, this trend was not followed by the respective enzyme activity. While the total SOD activity was induced by the stress, catalase activity remained unaltered. This discrepancy led us to a conclusion that chronic isolation stress may cause oxidant-antioxidant imbalance in rat liver tissue, favoring H(2)O(2) accumulation.",
journal = "Russian Journal of Physical Chemistry A",
title = "Antioxidant Enzymes Expression and Activity in Liver of Stressed Wistar Rat",
volume = "83",
number = "9",
pages = "1617-1620",
doi = "10.1134/S0036024409090362"
}

Đorđević, J. D., Nićiforović, A.,& Radojčić, M.. (2009). Antioxidant Enzymes Expression and Activity in Liver of Stressed Wistar Rat. in Russian Journal of Physical Chemistry A, 83(9), 1617-1620.
https://doi.org/10.1134/S0036024409090362

Đorđević JD, Nićiforović A, Radojčić M. Antioxidant Enzymes Expression and Activity in Liver of Stressed Wistar Rat. in Russian Journal of Physical Chemistry A. 2009;83(9):1617-1620.
doi:10.1134/S0036024409090362 .

Đorđević, Jelena D., Nićiforović, Ana, Radojčić, Marija, "Antioxidant Enzymes Expression and Activity in Liver of Stressed Wistar Rat" in Russian Journal of Physical Chemistry A, 83, no. 9 (2009):1617-1620,
https://doi.org/10.1134/S0036024409090362 . .

TY  - JOUR
AU  - Đorđević, Ana D.
AU  - Adžić, Miroslav
AU  - Đorđević, Jelena D.
AU  - Radojčić, Marija
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3762
AB  - Chronic exposure to stress is associated with different behavioral and neurological syndromes including impaired excitability of nerve cells in hippocampus (HIPPO) and prefrontal cortex (PFC), regions of the brain that are important for adaptation. The successful adaptation to stress involves negative feedback at the level of the hypothalamic-pituitary-adrenal (HPA) axis provided by the glucocorticoid receptor (GR), which is a steroid-dependent transcription factor found in a heterocomplex with heat shock proteins Hsp90 and Hsp70. In Wistar rats, chronic social isolation leads to a significant decrease in serum corticosterone (CORT), probably due to alterations in the GR signaling pathway. We exploited this type of stress, alone or in combination with acute immobilization, to define changes in the expression level and compartmental distribution of GR, Hsp90 and Hsp70 in HIPPO and PFC. The results indicated that in acute and combined stress, when CORT was increased, GR was translocated to the nucleus in both brain structures. Under chronic stress, when CORT was below the control level, GR was retained in the cytoplasm of PFC, and evenly distributed between compartments in HIPPO. Simultaneously, heat shock proteins partitioning in HIPPO seemed to be mainly stress type-independent, while that of PFC was dependent on stress type. Thus, the stress type-specific responses of GR and heat shock proteins were mainly detected in PFC rather than in HIPPO of Wistar rats. The observed alterations in protein expression and cytoplasmic-nuclear partitioning of the GR, Hsp90 and Hsp70 proteins may be related to maladaptive response of the HPA axis under chronic stress. Copyright (C) 2009 S. Karger AG, Basel
T2  - Neuropsychobiology
T1  - Stress Type Dependence of Expression and Cytoplasmic-Nuclear Partitioning of Glucocorticoid Receptor, Hsp90 and Hsp70 in Wistar Rat Brain
VL  - 59
IS  - 4
SP  - 213
EP  - 221
DO  - 10.1159/000223733
ER  - 

@article{
author = "Đorđević, Ana D. and Adžić, Miroslav and Đorđević, Jelena D. and Radojčić, Marija",
year = "2009",
abstract = "Chronic exposure to stress is associated with different behavioral and neurological syndromes including impaired excitability of nerve cells in hippocampus (HIPPO) and prefrontal cortex (PFC), regions of the brain that are important for adaptation. The successful adaptation to stress involves negative feedback at the level of the hypothalamic-pituitary-adrenal (HPA) axis provided by the glucocorticoid receptor (GR), which is a steroid-dependent transcription factor found in a heterocomplex with heat shock proteins Hsp90 and Hsp70. In Wistar rats, chronic social isolation leads to a significant decrease in serum corticosterone (CORT), probably due to alterations in the GR signaling pathway. We exploited this type of stress, alone or in combination with acute immobilization, to define changes in the expression level and compartmental distribution of GR, Hsp90 and Hsp70 in HIPPO and PFC. The results indicated that in acute and combined stress, when CORT was increased, GR was translocated to the nucleus in both brain structures. Under chronic stress, when CORT was below the control level, GR was retained in the cytoplasm of PFC, and evenly distributed between compartments in HIPPO. Simultaneously, heat shock proteins partitioning in HIPPO seemed to be mainly stress type-independent, while that of PFC was dependent on stress type. Thus, the stress type-specific responses of GR and heat shock proteins were mainly detected in PFC rather than in HIPPO of Wistar rats. The observed alterations in protein expression and cytoplasmic-nuclear partitioning of the GR, Hsp90 and Hsp70 proteins may be related to maladaptive response of the HPA axis under chronic stress. Copyright (C) 2009 S. Karger AG, Basel",
journal = "Neuropsychobiology",
title = "Stress Type Dependence of Expression and Cytoplasmic-Nuclear Partitioning of Glucocorticoid Receptor, Hsp90 and Hsp70 in Wistar Rat Brain",
volume = "59",
number = "4",
pages = "213-221",
doi = "10.1159/000223733"
}

Đorđević, A. D., Adžić, M., Đorđević, J. D.,& Radojčić, M.. (2009). Stress Type Dependence of Expression and Cytoplasmic-Nuclear Partitioning of Glucocorticoid Receptor, Hsp90 and Hsp70 in Wistar Rat Brain. in Neuropsychobiology, 59(4), 213-221.
https://doi.org/10.1159/000223733

Đorđević AD, Adžić M, Đorđević JD, Radojčić M. Stress Type Dependence of Expression and Cytoplasmic-Nuclear Partitioning of Glucocorticoid Receptor, Hsp90 and Hsp70 in Wistar Rat Brain. in Neuropsychobiology. 2009;59(4):213-221.
doi:10.1159/000223733 .

Đorđević, Ana D., Adžić, Miroslav, Đorđević, Jelena D., Radojčić, Marija, "Stress Type Dependence of Expression and Cytoplasmic-Nuclear Partitioning of Glucocorticoid Receptor, Hsp90 and Hsp70 in Wistar Rat Brain" in Neuropsychobiology, 59, no. 4 (2009):213-221,
https://doi.org/10.1159/000223733 . .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Đorđević, Ana D.
AU  - Krstić-Demonacos, Marija
AU  - Radojčić, Marija
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3688
AB  - Glucocorticoids are key regulators of the neuroendocrine stress response in the hippocampus. Their action is partly mediated through the subfamily of MAPKs termed c-jun-N-terminal kinases (JNKs), whose activation correlates with neurodegeneration. The stress response also involves activation of cell protective mechanisms through various heat shock proteins (HSPs) that mediate neuroprotection. We followed both JNKs and Hsp70 signals in the cytoplasmic and nuclear compartments of the hippocampus of Wistar male rats exposed to acute, chronic, and combined stress. The activity of JNK1 was decreased in both compartments by all three types of stress, while the activity of cytoplasmic JNK2/3 was elevated in acute and unaltered or lowered in chronic and combined stress. Under all stress conditions, Hsp70 translocation to the nucleus was markedly increased. The results suggest that neurodegenerative signaling of JNKs may be counteracted by increase of nuclear Hsp70, especially under chronic stress.
T2  - Archives of Biological Sciences
T1  - Stress-Induced Phosphorylation of C-JUN-N-Terminal Kinases and Nuclear Translocation of Hsp70 in the Wistar Rat Hippocampus
VL  - 61
IS  - 1
SP  - 1
EP  - 8
DO  - 10.2298/ABS0901001A
ER  - 

@article{
author = "Adžić, Miroslav and Đorđević, Ana D. and Krstić-Demonacos, Marija and Radojčić, Marija",
year = "2009",
abstract = "Glucocorticoids are key regulators of the neuroendocrine stress response in the hippocampus. Their action is partly mediated through the subfamily of MAPKs termed c-jun-N-terminal kinases (JNKs), whose activation correlates with neurodegeneration. The stress response also involves activation of cell protective mechanisms through various heat shock proteins (HSPs) that mediate neuroprotection. We followed both JNKs and Hsp70 signals in the cytoplasmic and nuclear compartments of the hippocampus of Wistar male rats exposed to acute, chronic, and combined stress. The activity of JNK1 was decreased in both compartments by all three types of stress, while the activity of cytoplasmic JNK2/3 was elevated in acute and unaltered or lowered in chronic and combined stress. Under all stress conditions, Hsp70 translocation to the nucleus was markedly increased. The results suggest that neurodegenerative signaling of JNKs may be counteracted by increase of nuclear Hsp70, especially under chronic stress.",
journal = "Archives of Biological Sciences",
title = "Stress-Induced Phosphorylation of C-JUN-N-Terminal Kinases and Nuclear Translocation of Hsp70 in the Wistar Rat Hippocampus",
volume = "61",
number = "1",
pages = "1-8",
doi = "10.2298/ABS0901001A"
}

Adžić, M., Đorđević, A. D., Krstić-Demonacos, M.,& Radojčić, M.. (2009). Stress-Induced Phosphorylation of C-JUN-N-Terminal Kinases and Nuclear Translocation of Hsp70 in the Wistar Rat Hippocampus. in Archives of Biological Sciences, 61(1), 1-8.
https://doi.org/10.2298/ABS0901001A

Adžić M, Đorđević AD, Krstić-Demonacos M, Radojčić M. Stress-Induced Phosphorylation of C-JUN-N-Terminal Kinases and Nuclear Translocation of Hsp70 in the Wistar Rat Hippocampus. in Archives of Biological Sciences. 2009;61(1):1-8.
doi:10.2298/ABS0901001A .

Adžić, Miroslav, Đorđević, Ana D., Krstić-Demonacos, Marija, Radojčić, Marija, "Stress-Induced Phosphorylation of C-JUN-N-Terminal Kinases and Nuclear Translocation of Hsp70 in the Wistar Rat Hippocampus" in Archives of Biological Sciences, 61, no. 1 (2009):1-8,
https://doi.org/10.2298/ABS0901001A . .

TY  - CONF
AU  - Đorđević, Jelena
AU  - Adžić, Miroslav
AU  - Đorđević, A.
AU  - Nićiforović, Ana
AU  - Radojčić, Marija B.
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9378
AB  - The antioxidant enzymes (AOEs) expression was studied in Wistar rat liver under two types of stress: acute (immobilization) and chronic (isolation). The acute stress induced increase in blood corticosterone (CORT) and glucose (GLU), but decreased AOEs expression, and such conditions may result in oxidative stress. In contrast to acute stress, in chronic stress conditions, when both CORT and GLU were low, the AOEs expression was markedly induced. This increase in MnSOD, CuZnSOD, and catalase exhibited similar trend implying efficient detoxification of O2 .- and H2O2.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
T1  - Antioxidant enzymes expression in liver of stressed wistar rat
VL  - 1
SP  - 441
EP  - 443
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9378
ER  - 

@conference{
author = "Đorđević, Jelena and Adžić, Miroslav and Đorđević, A. and Nićiforović, Ana and Radojčić, Marija B.",
year = "2008",
abstract = "The antioxidant enzymes (AOEs) expression was studied in Wistar rat liver under two types of stress: acute (immobilization) and chronic (isolation). The acute stress induced increase in blood corticosterone (CORT) and glucose (GLU), but decreased AOEs expression, and such conditions may result in oxidative stress. In contrast to acute stress, in chronic stress conditions, when both CORT and GLU were low, the AOEs expression was markedly induced. This increase in MnSOD, CuZnSOD, and catalase exhibited similar trend implying efficient detoxification of O2 .- and H2O2.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry",
title = "Antioxidant enzymes expression in liver of stressed wistar rat",
volume = "1",
pages = "441-443",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9378"
}

Đorđević, J., Adžić, M., Đorđević, A., Nićiforović, A.,& Radojčić, M. B.. (2008). Antioxidant enzymes expression in liver of stressed wistar rat. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 441-443.
https://hdl.handle.net/21.15107/rcub_vinar_9378

Đorđević J, Adžić M, Đorđević A, Nićiforović A, Radojčić MB. Antioxidant enzymes expression in liver of stressed wistar rat. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry. 2008;1:441-443.
https://hdl.handle.net/21.15107/rcub_vinar_9378 .

Đorđević, Jelena, Adžić, Miroslav, Đorđević, A., Nićiforović, Ana, Radojčić, Marija B., "Antioxidant enzymes expression in liver of stressed wistar rat" in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry, 1 (2008):441-443,
https://hdl.handle.net/21.15107/rcub_vinar_9378 .

TY  - CONF
AU  - Adžić, Miroslav
AU  - Đorđević, A.
AU  - Đorđević, Jelena
AU  - Krstić-Demonacos, Marija
AU  - Radojčić, Marija
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9377
AB  - Glucocorticoids have diverse effects in cellular processes in hippocampus (HIPPO) under stress. Beside genomic pathways, their effects are also mediated by direct activation of subfamily of mitogen-activated protein kinases termed, c-Jun-Nterminal kinases (JNKs). We analysed the phosphorylation status of cytoplasmic and nuclear JNK isoforms, and expression of its inhibitor Hsp70 protein in HIPPO of rats exposed to diverse types of stress. Activity of JNK1 in cytoplasm and nucleus was decreased in all types of stress, while the activity of cytoplasmic JNK2/3 was markedly higher in acute stress, and unaltered or lowered in chronic and combined stress. Hsp70 was significantly decreased in cytoplasm and increased in nucleus under all stress conditions indicating its cytoplasmic-nuclear translocation.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
T1  - Stress effects on the phosphorylation of c-jun-nterminal kinases and on nuclear translocation of hsp70 in rat hippocampus
VL  - 1
SP  - 438
EP  - 440
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9377
ER  - 

@conference{
author = "Adžić, Miroslav and Đorđević, A. and Đorđević, Jelena and Krstić-Demonacos, Marija and Radojčić, Marija",
year = "2008",
abstract = "Glucocorticoids have diverse effects in cellular processes in hippocampus (HIPPO) under stress. Beside genomic pathways, their effects are also mediated by direct activation of subfamily of mitogen-activated protein kinases termed, c-Jun-Nterminal kinases (JNKs). We analysed the phosphorylation status of cytoplasmic and nuclear JNK isoforms, and expression of its inhibitor Hsp70 protein in HIPPO of rats exposed to diverse types of stress. Activity of JNK1 in cytoplasm and nucleus was decreased in all types of stress, while the activity of cytoplasmic JNK2/3 was markedly higher in acute stress, and unaltered or lowered in chronic and combined stress. Hsp70 was significantly decreased in cytoplasm and increased in nucleus under all stress conditions indicating its cytoplasmic-nuclear translocation.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry",
title = "Stress effects on the phosphorylation of c-jun-nterminal kinases and on nuclear translocation of hsp70 in rat hippocampus",
volume = "1",
pages = "438-440",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9377"
}

Adžić, M., Đorđević, A., Đorđević, J., Krstić-Demonacos, M.,& Radojčić, M.. (2008). Stress effects on the phosphorylation of c-jun-nterminal kinases and on nuclear translocation of hsp70 in rat hippocampus. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 438-440.
https://hdl.handle.net/21.15107/rcub_vinar_9377

Adžić M, Đorđević A, Đorđević J, Krstić-Demonacos M, Radojčić M. Stress effects on the phosphorylation of c-jun-nterminal kinases and on nuclear translocation of hsp70 in rat hippocampus. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry. 2008;1:438-440.
https://hdl.handle.net/21.15107/rcub_vinar_9377 .

Adžić, Miroslav, Đorđević, A., Đorđević, Jelena, Krstić-Demonacos, Marija, Radojčić, Marija, "Stress effects on the phosphorylation of c-jun-nterminal kinases and on nuclear translocation of hsp70 in rat hippocampus" in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry, 1 (2008):438-440,
https://hdl.handle.net/21.15107/rcub_vinar_9377 .

TY  - CONF
AU  - Đorđević, A.
AU  - Adžić, Miroslav
AU  - Đorđević, Jelena
AU  - Radojčić, Marija
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9376
AB  - Hippocampus (HIPPO) is one of the key brain structures, rich in glucocorticoid receptor (GR), a transcriptional factor involved in negative feedback of hypothalamic- pituitary-adrenal (HPA) axis in response to stress. Heat shock proteins accompany GR maintaining its optimal conformation, ligand binding ability and translocation to the nucleus. In order to evaluate the expression of GR, Hsp90 and their ratio we exploited three diverse types of stress (acute immobilization, chronic isolation and combination of the two). Our results indicated the same pattern of expression and compartmental distribution for both proteins, as well as for their ratio, under acute and combined stress when the level of corticosterone (CORT) was high. On the contrary, when CORT was low, such as in chronic stress, Hsp90/GR ratio exhibited opposite pattern of expression and GR was not translocated to the nucleus.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
T1  - Corticosterone level alters optimal heat shock protein 90/glucocorticoid receptor ratio in hippocampus of stressed rats
VL  - 1
SP  - 435
EP  - 437
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9376
ER  - 

@conference{
author = "Đorđević, A. and Adžić, Miroslav and Đorđević, Jelena and Radojčić, Marija",
year = "2008",
abstract = "Hippocampus (HIPPO) is one of the key brain structures, rich in glucocorticoid receptor (GR), a transcriptional factor involved in negative feedback of hypothalamic- pituitary-adrenal (HPA) axis in response to stress. Heat shock proteins accompany GR maintaining its optimal conformation, ligand binding ability and translocation to the nucleus. In order to evaluate the expression of GR, Hsp90 and their ratio we exploited three diverse types of stress (acute immobilization, chronic isolation and combination of the two). Our results indicated the same pattern of expression and compartmental distribution for both proteins, as well as for their ratio, under acute and combined stress when the level of corticosterone (CORT) was high. On the contrary, when CORT was low, such as in chronic stress, Hsp90/GR ratio exhibited opposite pattern of expression and GR was not translocated to the nucleus.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry",
title = "Corticosterone level alters optimal heat shock protein 90/glucocorticoid receptor ratio in hippocampus of stressed rats",
volume = "1",
pages = "435-437",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9376"
}

Đorđević, A., Adžić, M., Đorđević, J.,& Radojčić, M.. (2008). Corticosterone level alters optimal heat shock protein 90/glucocorticoid receptor ratio in hippocampus of stressed rats. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 435-437.
https://hdl.handle.net/21.15107/rcub_vinar_9376

Đorđević A, Adžić M, Đorđević J, Radojčić M. Corticosterone level alters optimal heat shock protein 90/glucocorticoid receptor ratio in hippocampus of stressed rats. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry. 2008;1:435-437.
https://hdl.handle.net/21.15107/rcub_vinar_9376 .

Đorđević, A., Adžić, Miroslav, Đorđević, Jelena, Radojčić, Marija, "Corticosterone level alters optimal heat shock protein 90/glucocorticoid receptor ratio in hippocampus of stressed rats" in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry, 1 (2008):435-437,
https://hdl.handle.net/21.15107/rcub_vinar_9376 .

TY  - JOUR
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Spasić, Snežana D.
AU  - Radojčić, Marija
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3307
AB  - Most experimental models, including cell culture studies, have demonstrated that over-expression of manganese superoxide dismutase (MnSOD) in cells bearing a carcinoma phenotype has anti-proliferative and tumour suppression characteristics. In contrast, when cervical carcinoma biopsies express MnSOD, there is a poor prognosis and resistance to radiation therapy. The results herein indicate that human cervical adenocarcinoma (HeLaS3) cells have increased MnSOD activity (up to 50 % of the total SOD activity) due to low expression of its repressor p53 and a high level of oxidative stress arising from the cell culture conditions. High MnSOD activity may be related to I-IcLaS3 cell radioresistance. illustrated by a high IC50 of 3.4 Gy and by a relatively high level of cell viability after gamma irradiation. In contrast to MnSOD activity, cytosolic CuZnSOD activity decreased after ionising radiation. The catalase (Cat) activity was unchanged. IR also increased the nitric oxide synthase (NOS) activity. Such conditions lead to increased concentrations of the superoxide radical, hydrogen peroxide and NO, which together may be responsible for the decreased expression of NF-B-K and unaltered Cat activity. Therefore, the disturbed redox balance within HeLaS3 cells may be responsible for the cytotoxicity observed at higher irradiation doses. It could be concluded that inhibition of the CuZrISOD activity may be an important target for the selective killing of radioresistant cancer cells.
T2  - Journal of the Serbian Chemical Society
T1  - Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?
VL  - 72
IS  - 10
SP  - 945
EP  - 952
DO  - 10.2298/JSC0710945N
ER  - 

@article{
author = "Nićiforović, Ana and Adžić, Miroslav and Spasić, Snežana D. and Radojčić, Marija",
year = "2007",
abstract = "Most experimental models, including cell culture studies, have demonstrated that over-expression of manganese superoxide dismutase (MnSOD) in cells bearing a carcinoma phenotype has anti-proliferative and tumour suppression characteristics. In contrast, when cervical carcinoma biopsies express MnSOD, there is a poor prognosis and resistance to radiation therapy. The results herein indicate that human cervical adenocarcinoma (HeLaS3) cells have increased MnSOD activity (up to 50 % of the total SOD activity) due to low expression of its repressor p53 and a high level of oxidative stress arising from the cell culture conditions. High MnSOD activity may be related to I-IcLaS3 cell radioresistance. illustrated by a high IC50 of 3.4 Gy and by a relatively high level of cell viability after gamma irradiation. In contrast to MnSOD activity, cytosolic CuZnSOD activity decreased after ionising radiation. The catalase (Cat) activity was unchanged. IR also increased the nitric oxide synthase (NOS) activity. Such conditions lead to increased concentrations of the superoxide radical, hydrogen peroxide and NO, which together may be responsible for the decreased expression of NF-B-K and unaltered Cat activity. Therefore, the disturbed redox balance within HeLaS3 cells may be responsible for the cytotoxicity observed at higher irradiation doses. It could be concluded that inhibition of the CuZrISOD activity may be an important target for the selective killing of radioresistant cancer cells.",
journal = "Journal of the Serbian Chemical Society",
title = "Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?",
volume = "72",
number = "10",
pages = "945-952",
doi = "10.2298/JSC0710945N"
}

Nićiforović, A., Adžić, M., Spasić, S. D.,& Radojčić, M.. (2007). Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?. in Journal of the Serbian Chemical Society, 72(10), 945-952.
https://doi.org/10.2298/JSC0710945N

Nićiforović A, Adžić M, Spasić SD, Radojčić M. Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?. in Journal of the Serbian Chemical Society. 2007;72(10):945-952.
doi:10.2298/JSC0710945N .

Nićiforović, Ana, Adžić, Miroslav, Spasić, Snežana D., Radojčić, Marija, "Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?" in Journal of the Serbian Chemical Society, 72, no. 10 (2007):945-952,
https://doi.org/10.2298/JSC0710945N . .