TY  - JOUR
AU  - Houdova, Dominika
AU  - Popović, Iva A.
AU  - Dellinger, Thomas
AU  - Nešić, Maja D.
AU  - Petković, Marijana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10135
AB  - The endangered loggerhead sea turtles (Caretta caretta) are a relatively abundant sea turtle species in Madeiran waters, where they encounter various environmental stressors: from natural to anthropogenic. The physiological stress response is increased corticosterone (CS) level in the blood, achieving 2 ng/mL. Although there are various analytical methods for determining the CS concentration in turtle blood, most of them require tedious procedures for the preparation. In this work, we have tested the possibility of using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) for fast screening and detection of CS in blood from a turtle captured at the coast of Madeira Island. MALDI does not require sample derivatization and is suitable for low sample volume. The linearity, precision, and limit of detection and quantification with three organic MALDI matrices were investigated in this study. The signal-to-noise ratio of the CS-derived signal was used as a parameter for quantification. Isotopically labelled corticosterone was added as a correction factor at fixed concentration to achieve better linearity and precision. Our results demonstrate that this method has the potential for quantification of CS in turtle blood. However, the lowest concentration of CS extracted from plasma that was still detectable by MALDI TOF MS was about 0.04 mg/mL, which is about ten times higher than the expected CS concentration in blood.
T2  - International Journal of Mass Spectrometry
T1  - Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles
VL  - 473
SP  - 116796
DO  - 10.1016/j.ijms.2022.116796
ER  - 

@article{
author = "Houdova, Dominika and Popović, Iva A. and Dellinger, Thomas and Nešić, Maja D. and Petković, Marijana",
year = "2022",
abstract = "The endangered loggerhead sea turtles (Caretta caretta) are a relatively abundant sea turtle species in Madeiran waters, where they encounter various environmental stressors: from natural to anthropogenic. The physiological stress response is increased corticosterone (CS) level in the blood, achieving 2 ng/mL. Although there are various analytical methods for determining the CS concentration in turtle blood, most of them require tedious procedures for the preparation. In this work, we have tested the possibility of using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) for fast screening and detection of CS in blood from a turtle captured at the coast of Madeira Island. MALDI does not require sample derivatization and is suitable for low sample volume. The linearity, precision, and limit of detection and quantification with three organic MALDI matrices were investigated in this study. The signal-to-noise ratio of the CS-derived signal was used as a parameter for quantification. Isotopically labelled corticosterone was added as a correction factor at fixed concentration to achieve better linearity and precision. Our results demonstrate that this method has the potential for quantification of CS in turtle blood. However, the lowest concentration of CS extracted from plasma that was still detectable by MALDI TOF MS was about 0.04 mg/mL, which is about ten times higher than the expected CS concentration in blood.",
journal = "International Journal of Mass Spectrometry",
title = "Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles",
volume = "473",
pages = "116796",
doi = "10.1016/j.ijms.2022.116796"
}

Houdova, D., Popović, I. A., Dellinger, T., Nešić, M. D.,& Petković, M.. (2022). Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles. in International Journal of Mass Spectrometry, 473, 116796.
https://doi.org/10.1016/j.ijms.2022.116796

Houdova D, Popović IA, Dellinger T, Nešić MD, Petković M. Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles. in International Journal of Mass Spectrometry. 2022;473:116796.
doi:10.1016/j.ijms.2022.116796 .

Houdova, Dominika, Popović, Iva A., Dellinger, Thomas, Nešić, Maja D., Petković, Marijana, "Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles" in International Journal of Mass Spectrometry, 473 (2022):116796,
https://doi.org/10.1016/j.ijms.2022.116796 . .

TY  - JOUR
AU  - Nešić, Maja D.
AU  - Dučić, Tanja
AU  - Algarra, Manuel
AU  - Popović, Iva A.
AU  - Stepić, Milutin
AU  - Gonçalves, Mara
AU  - Petković, Marijana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10184
AB  - In the last decade, targeting membrane lipids in cancer cells has been a promising approach that deserves attention in the field of anticancer drug development. To get a comprehensive understanding of the effect of the drug [Ru(η5-Cp)(PPh3)2CN] (RuCN) on cell lipidic components, we combine complementary analytical approaches, matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) and synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy. Techniques are used for screening the effect of potential metallodrug, RuCN, without and with drug carriers (carbon dots (CDs) and nitrogen-doped carbon dots (N-CDs)) on the lipids of the human ovarian cancer cell line A2780. MALDI TOF MS results revealed that the lysis of ovarian cancer membrane lipids is promoted by RuCN and not by drug carriers (CDs and N-CDs). Furthermore, SR-FTIR results strongly suggested that the phospholipids of cancer cells undergo oxidative stress after the treatment with RuCN that was accompanied by the disordering of the fatty acid chains. On the other hand, using (N-)CDs as RuCN nanocarriers prevented the oxidative stress caused by RuCN but did not prevent the disordering of the fatty acid chain packing. Finally, we demonstrated that RuCN and RuCN/(N-)CDs alter the hydration of the membrane surface in the membrane–water interface region.
T2  - Cancers
T1  - Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study
VL  - 14
IS  - 5
SP  - 1182
DO  - 10.3390/cancers14051182
ER  - 

@article{
author = "Nešić, Maja D. and Dučić, Tanja and Algarra, Manuel and Popović, Iva A. and Stepić, Milutin and Gonçalves, Mara and Petković, Marijana",
year = "2022",
abstract = "In the last decade, targeting membrane lipids in cancer cells has been a promising approach that deserves attention in the field of anticancer drug development. To get a comprehensive understanding of the effect of the drug [Ru(η5-Cp)(PPh3)2CN] (RuCN) on cell lipidic components, we combine complementary analytical approaches, matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) and synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy. Techniques are used for screening the effect of potential metallodrug, RuCN, without and with drug carriers (carbon dots (CDs) and nitrogen-doped carbon dots (N-CDs)) on the lipids of the human ovarian cancer cell line A2780. MALDI TOF MS results revealed that the lysis of ovarian cancer membrane lipids is promoted by RuCN and not by drug carriers (CDs and N-CDs). Furthermore, SR-FTIR results strongly suggested that the phospholipids of cancer cells undergo oxidative stress after the treatment with RuCN that was accompanied by the disordering of the fatty acid chains. On the other hand, using (N-)CDs as RuCN nanocarriers prevented the oxidative stress caused by RuCN but did not prevent the disordering of the fatty acid chain packing. Finally, we demonstrated that RuCN and RuCN/(N-)CDs alter the hydration of the membrane surface in the membrane–water interface region.",
journal = "Cancers",
title = "Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study",
volume = "14",
number = "5",
pages = "1182",
doi = "10.3390/cancers14051182"
}

Nešić, M. D., Dučić, T., Algarra, M., Popović, I. A., Stepić, M., Gonçalves, M.,& Petković, M.. (2022). Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study. in Cancers, 14(5), 1182.
https://doi.org/10.3390/cancers14051182

Nešić MD, Dučić T, Algarra M, Popović IA, Stepić M, Gonçalves M, Petković M. Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study. in Cancers. 2022;14(5):1182.
doi:10.3390/cancers14051182 .

Nešić, Maja D., Dučić, Tanja, Algarra, Manuel, Popović, Iva A., Stepić, Milutin, Gonçalves, Mara, Petković, Marijana, "Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study" in Cancers, 14, no. 5 (2022):1182,
https://doi.org/10.3390/cancers14051182 . .

TY  - JOUR
AU  - Dučić, Tanja
AU  - Alves, Carla S.
AU  - Vučinić, Željko
AU  - Lázaro-Martínez, Juan M.
AU  - Petković, Marijana
AU  - Soto, Juan
AU  - Mutavdžić, Dragosav
AU  - Valle Martínez de Yuso, M.
AU  - Radotić, Ksenija
AU  - Algarra, Manuel
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10277
AB  - S and N-doped carbon dots (S-CDs and N-CDs) and their cisplatin (cis-Pt) derivatives. (S-CDs@cis-Pt and N-CDs@cis-Pt) were tested on two ovarian cancer cell lines: A2780 and A2780 cells resistant to cis-Pt (A2780R). Several spectroscopic techniques were employed to check S-CDs@cis-Pt and N-CDs@cis-Pt: solid- and solution-state nuclear magnetic resonance, matrix-assisted laser desorption, ionization time-of-flight mass spectrometry, and X-ray photoelectron spectroscopy. In addition, synchrotron-based Fourier Transformed Infrared spectro-microscopy was used to evaluate the biochemical changes in cells after treatment with cis-Pt, S-CDs, N-CDs, or S-CDs@cis-Pt and N-CDs@cis-Pt, respectively. Computational chemistry was applied to establish the model for the most stable bond between S-CDs and N-CDs and cis-Pt. The results revealed the successful modification of S-CDs and N-CDs with cis-Pt and the formation of a stable composite system that can be used for drug delivery to cancer cells and likewise to overcome acquired cis-Pt resistance. Nanoparticle treatment of A2780 and A2780R cells led to the changes in their structure of lipids, proteins, and nucleic acids depending on the treatment. The results showed the S-CDs@cis-Pt and N-CDs@cis-Pt might be used in the combination with cis-Pt to treat the adenocarcinoma, thus having a potential to be further developed as drug delivery systems.
T2  - Journal of Colloid and Interface Science
T1  - S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach
VL  - 623
SP  - 226
EP  - 237
DO  - 10.1016/j.jcis.2022.05.005
ER  - 

@article{
author = "Dučić, Tanja and Alves, Carla S. and Vučinić, Željko and Lázaro-Martínez, Juan M. and Petković, Marijana and Soto, Juan and Mutavdžić, Dragosav and Valle Martínez de Yuso, M. and Radotić, Ksenija and Algarra, Manuel",
year = "2022",
abstract = "S and N-doped carbon dots (S-CDs and N-CDs) and their cisplatin (cis-Pt) derivatives. (S-CDs@cis-Pt and N-CDs@cis-Pt) were tested on two ovarian cancer cell lines: A2780 and A2780 cells resistant to cis-Pt (A2780R). Several spectroscopic techniques were employed to check S-CDs@cis-Pt and N-CDs@cis-Pt: solid- and solution-state nuclear magnetic resonance, matrix-assisted laser desorption, ionization time-of-flight mass spectrometry, and X-ray photoelectron spectroscopy. In addition, synchrotron-based Fourier Transformed Infrared spectro-microscopy was used to evaluate the biochemical changes in cells after treatment with cis-Pt, S-CDs, N-CDs, or S-CDs@cis-Pt and N-CDs@cis-Pt, respectively. Computational chemistry was applied to establish the model for the most stable bond between S-CDs and N-CDs and cis-Pt. The results revealed the successful modification of S-CDs and N-CDs with cis-Pt and the formation of a stable composite system that can be used for drug delivery to cancer cells and likewise to overcome acquired cis-Pt resistance. Nanoparticle treatment of A2780 and A2780R cells led to the changes in their structure of lipids, proteins, and nucleic acids depending on the treatment. The results showed the S-CDs@cis-Pt and N-CDs@cis-Pt might be used in the combination with cis-Pt to treat the adenocarcinoma, thus having a potential to be further developed as drug delivery systems.",
journal = "Journal of Colloid and Interface Science",
title = "S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach",
volume = "623",
pages = "226-237",
doi = "10.1016/j.jcis.2022.05.005"
}

Dučić, T., Alves, C. S., Vučinić, Ž., Lázaro-Martínez, J. M., Petković, M., Soto, J., Mutavdžić, D., Valle Martínez de Yuso, M., Radotić, K.,& Algarra, M.. (2022). S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach. in Journal of Colloid and Interface Science, 623, 226-237.
https://doi.org/10.1016/j.jcis.2022.05.005

Dučić T, Alves CS, Vučinić Ž, Lázaro-Martínez JM, Petković M, Soto J, Mutavdžić D, Valle Martínez de Yuso M, Radotić K, Algarra M. S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach. in Journal of Colloid and Interface Science. 2022;623:226-237.
doi:10.1016/j.jcis.2022.05.005 .

Dučić, Tanja, Alves, Carla S., Vučinić, Željko, Lázaro-Martínez, Juan M., Petković, Marijana, Soto, Juan, Mutavdžić, Dragosav, Valle Martínez de Yuso, M., Radotić, Ksenija, Algarra, Manuel, "S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach" in Journal of Colloid and Interface Science, 623 (2022):226-237,
https://doi.org/10.1016/j.jcis.2022.05.005 . .