Modulation of antioxidative metabolism in plants for improvement of plant abiotic stress tolerance and identification of new biomarkers for application in remediation and monitoring of degraded biotopes

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Modulation of antioxidative metabolism in plants for improvement of plant abiotic stress tolerance and identification of new biomarkers for application in remediation and monitoring of degraded biotopes (en)
Модификације антиоксидативног метаболизма биљака са циљем повећања толеранције на абиотски стрес и идентификација нових биомаркера са применом у ремедијацији и мониторингу деградираних станишта (sr)
Modifikacije antioksidativnog metabolizma biljaka sa ciljem povećanja tolerancije na abiotski stres i identifikacija novih biomarkera sa primenom u remedijaciji i monitoringu degradiranih staništa (sr_RS)
Authors

Publications

Biliverdin-copper complex at physiological pH

Dimitrijević, Milena S.; Bogdanović-Pristov, Jelena; Žižić, Milan; Stanković, Dalibor M.; Bajuk-Bogdanović, Danica V.; Stanić, Marina; Spasić, Snežana D.; Hagen, Wilfred; Spasojević, Ivan

(2019)

TY  - JOUR
AU  - Dimitrijević, Milena S.
AU  - Bogdanović-Pristov, Jelena
AU  - Žižić, Milan
AU  - Stanković, Dalibor M.
AU  - Bajuk-Bogdanović, Danica V.
AU  - Stanić, Marina
AU  - Spasić, Snežana D.
AU  - Hagen, Wilfred
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8258
AB  - Biliverdin (BV), a product of heme catabolism, is known to interact with transition metals, but the details of such interactions under physiological conditions are scarce. Herein, we examined coordinate/redox interactions of BV with Cu2+ in phosphate buffer at pH 7.4, using spectrophotometry, HESI-MS, Raman spectroscopy, 1 H NMR, EPR, fluorimetry, and electrochemical methods. BV formed a stable coordination complex with copper in 1 : 1 stoichiometry. The structure of BV was more planar and energetically stable in the complex. The complex showed strong paramagnetic effects that were attributed to an unpaired delocalized e−. The delocalized electron may come from BV or Cu2+, so the complex is formally composed either of BV radical cation and Cu1+ or of BV radical anion and Cu3+. The complex underwent oxidation only in the presence of both O2 and an excess of Cu2+, or a strong oxidizing agent, and it was resistant to reducing agents. The biological effects of the stable BV metallocomplex containing a delocalized unpaired electron should be further examined, and may provide an answer to the long-standing question of high energy investment in the catabolism of BV, which represents a relatively harmless molecule per se.
T2  - Dalton Transactions
T1  - Biliverdin-copper complex at physiological pH
VL  - 48
IS  - 18
SP  - 6061
EP  - 6070
DO  - 10.1039/c8dt04724c
ER  - 
@article{
author = "Dimitrijević, Milena S. and Bogdanović-Pristov, Jelena and Žižić, Milan and Stanković, Dalibor M. and Bajuk-Bogdanović, Danica V. and Stanić, Marina and Spasić, Snežana D. and Hagen, Wilfred and Spasojević, Ivan",
year = "2019",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/8258",
abstract = "Biliverdin (BV), a product of heme catabolism, is known to interact with transition metals, but the details of such interactions under physiological conditions are scarce. Herein, we examined coordinate/redox interactions of BV with Cu2+ in phosphate buffer at pH 7.4, using spectrophotometry, HESI-MS, Raman spectroscopy, 1 H NMR, EPR, fluorimetry, and electrochemical methods. BV formed a stable coordination complex with copper in 1 : 1 stoichiometry. The structure of BV was more planar and energetically stable in the complex. The complex showed strong paramagnetic effects that were attributed to an unpaired delocalized e−. The delocalized electron may come from BV or Cu2+, so the complex is formally composed either of BV radical cation and Cu1+ or of BV radical anion and Cu3+. The complex underwent oxidation only in the presence of both O2 and an excess of Cu2+, or a strong oxidizing agent, and it was resistant to reducing agents. The biological effects of the stable BV metallocomplex containing a delocalized unpaired electron should be further examined, and may provide an answer to the long-standing question of high energy investment in the catabolism of BV, which represents a relatively harmless molecule per se.",
journal = "Dalton Transactions",
title = "Biliverdin-copper complex at physiological pH",
volume = "48",
number = "18",
pages = "6061-6070",
doi = "10.1039/c8dt04724c"
}
Dimitrijević, M. S., Bogdanović-Pristov, J., Žižić, M., Stanković, D. M., Bajuk-Bogdanović, D. V., Stanić, M., Spasić, S. D., Hagen, W.,& Spasojević, I. (2019). Biliverdin-copper complex at physiological pH.
Dalton Transactions, 48(18), 6061-6070.
https://doi.org/10.1039/c8dt04724c
Dimitrijević MS, Bogdanović-Pristov J, Žižić M, Stanković DM, Bajuk-Bogdanović DV, Stanić M, Spasić SD, Hagen W, Spasojević I. Biliverdin-copper complex at physiological pH. Dalton Transactions. 2019;48(18):6061-6070
Dimitrijević Milena S., Bogdanović-Pristov Jelena, Žižić Milan, Stanković Dalibor M., Bajuk-Bogdanović Danica V., Stanić Marina, Spasić Snežana D., Hagen Wilfred, Spasojević Ivan, "Biliverdin-copper complex at physiological pH" Dalton Transactions, 48, no. 18 (2019):6061-6070,
https://doi.org/10.1039/c8dt04724c .
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Biliverdin-copper complex at physiological pH

Dimitrijević, Milena S.; Bogdanović-Pristov, Jelena; Žižić, Milan; Stanković, Dalibor M.; Bajuk-Bogdanović, Danica V.; Stanić, Marina; Spasić, Snežana D.; Hagen, Wilfred; Spasojević, Ivan

(2019)

TY  - JOUR
AU  - Dimitrijević, Milena S.
AU  - Bogdanović-Pristov, Jelena
AU  - Žižić, Milan
AU  - Stanković, Dalibor M.
AU  - Bajuk-Bogdanović, Danica V.
AU  - Stanić, Marina
AU  - Spasić, Snežana D.
AU  - Hagen, Wilfred
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/3066
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/8200
AB  - Biliverdin (BV), a product of heme catabolism, is known to interact with transition metals, but the details of such interactions under physiological conditions are scarce. Herein, we examined coordinate/redox interactions of BV with Cu2+ in phosphate buffer at pH 7.4, using spectrophotometry, HESI-MS, Raman spectroscopy, 1 H NMR, EPR, fluorimetry, and electrochemical methods. BV formed a stable coordination complex with copper in 1 : 1 stoichiometry. The structure of BV was more planar and energetically stable in the complex. The complex showed strong paramagnetic effects that were attributed to an unpaired delocalized e−. The delocalized electron may come from BV or Cu2+, so the complex is formally composed either of BV radical cation and Cu1+ or of BV radical anion and Cu3+. The complex underwent oxidation only in the presence of both O2 and an excess of Cu2+, or a strong oxidizing agent, and it was resistant to reducing agents. The biological effects of the stable BV metallocomplex containing a delocalized unpaired electron should be further examined, and may provide an answer to the long-standing question of high energy investment in the catabolism of BV, which represents a relatively harmless molecule per se.
T2  - Dalton Transactions
T1  - Biliverdin-copper complex at physiological pH
VL  - 48
IS  - 18
SP  - 6061
EP  - 6070
DO  - 10.1039/c8dt04724c
ER  - 
@article{
author = "Dimitrijević, Milena S. and Bogdanović-Pristov, Jelena and Žižić, Milan and Stanković, Dalibor M. and Bajuk-Bogdanović, Danica V. and Stanić, Marina and Spasić, Snežana D. and Hagen, Wilfred and Spasojević, Ivan",
year = "2019",
url = "http://cherry.chem.bg.ac.rs/handle/123456789/3066, http://vinar.vin.bg.ac.rs/handle/123456789/8200",
abstract = "Biliverdin (BV), a product of heme catabolism, is known to interact with transition metals, but the details of such interactions under physiological conditions are scarce. Herein, we examined coordinate/redox interactions of BV with Cu2+ in phosphate buffer at pH 7.4, using spectrophotometry, HESI-MS, Raman spectroscopy, 1 H NMR, EPR, fluorimetry, and electrochemical methods. BV formed a stable coordination complex with copper in 1 : 1 stoichiometry. The structure of BV was more planar and energetically stable in the complex. The complex showed strong paramagnetic effects that were attributed to an unpaired delocalized e−. The delocalized electron may come from BV or Cu2+, so the complex is formally composed either of BV radical cation and Cu1+ or of BV radical anion and Cu3+. The complex underwent oxidation only in the presence of both O2 and an excess of Cu2+, or a strong oxidizing agent, and it was resistant to reducing agents. The biological effects of the stable BV metallocomplex containing a delocalized unpaired electron should be further examined, and may provide an answer to the long-standing question of high energy investment in the catabolism of BV, which represents a relatively harmless molecule per se.",
journal = "Dalton Transactions",
title = "Biliverdin-copper complex at physiological pH",
volume = "48",
number = "18",
pages = "6061-6070",
doi = "10.1039/c8dt04724c"
}
Dimitrijević, M. S., Bogdanović-Pristov, J., Žižić, M., Stanković, D. M., Bajuk-Bogdanović, D. V., Stanić, M., Spasić, S. D., Hagen, W.,& Spasojević, I. (2019). Biliverdin-copper complex at physiological pH.
Dalton Transactions, 48(18), 6061-6070.
https://doi.org/10.1039/c8dt04724c
Dimitrijević MS, Bogdanović-Pristov J, Žižić M, Stanković DM, Bajuk-Bogdanović DV, Stanić M, Spasić SD, Hagen W, Spasojević I. Biliverdin-copper complex at physiological pH. Dalton Transactions. 2019;48(18):6061-6070
Dimitrijević Milena S., Bogdanović-Pristov Jelena, Žižić Milan, Stanković Dalibor M., Bajuk-Bogdanović Danica V., Stanić Marina, Spasić Snežana D., Hagen Wilfred, Spasojević Ivan, "Biliverdin-copper complex at physiological pH" Dalton Transactions, 48, no. 18 (2019):6061-6070,
https://doi.org/10.1039/c8dt04724c .
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Antioxidant activity of propolis extracts from Serbia: A polarographic approach

Potkonjak, Nebojša I.; Veselinović, Dragan S.; Novaković, Miroslav M.; Gorjanovic, Stanislava Z.; Pezo, Lato; Sužnjevicć, Desanka Ž.

(2012)

TY  - JOUR
AU  - Potkonjak, Nebojša I.
AU  - Veselinović, Dragan S.
AU  - Novaković, Miroslav M.
AU  - Gorjanovic, Stanislava Z.
AU  - Pezo, Lato
AU  - Sužnjevicć, Desanka Ž.
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5101
AB  - Antioxidant activity (AO) of commercial propolis extracts (PEs), available on Serbian market, was determined by direct current (DC) polarography. Polarographic anodic current of 5.0 mmol L-1 alkaline solution of H2O2 was recorded at potentials of mercury dissolution. Decrease of the current was plotted against the volume of gradually added PEs. The volume of PE causing 20% current decrease was determined from the linear part of the plot. Antioxidant activity was expressed in H2O2 equivalent (HPEq), representing the volume of PE that corresponds to 1.0 mmol L-1 H2O2 decrease. Resulting HPEq ranged between 1.71 +/- 0.11 and 8.00 +/- 0.18 mu L. Range of 1,1-dipheny1-2-picryl hydrazyl (DPPH) radical scavenging activity was from 0.093 +/- 0.004% to 0.346 +/- 0.006%. Total phenolic content (TCP) of PE with superior AO activity was 5.31 +/- 0.05%g GAE, while the extract with the lowest activity contained 1.45 +/- 0.02%g GAE. Antioxidant activity, determined by polarographic method, was correlated with DPPH scavenging activity (R-2 = 0.991) and TCP (R-2 = 0.985). Validity of obtained results was further confirmed using ANOVA and post hoc Tukey HSD test. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Antioxidant activity of propolis extracts from Serbia: A polarographic approach
VL  - 50
IS  - 10
SP  - 3614
EP  - 3618
DO  - 10.1016/j.fct.2012.07.029
ER  - 
@article{
author = "Potkonjak, Nebojša I. and Veselinović, Dragan S. and Novaković, Miroslav M. and Gorjanovic, Stanislava Z. and Pezo, Lato and Sužnjevicć, Desanka Ž.",
year = "2012",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5101",
abstract = "Antioxidant activity (AO) of commercial propolis extracts (PEs), available on Serbian market, was determined by direct current (DC) polarography. Polarographic anodic current of 5.0 mmol L-1 alkaline solution of H2O2 was recorded at potentials of mercury dissolution. Decrease of the current was plotted against the volume of gradually added PEs. The volume of PE causing 20% current decrease was determined from the linear part of the plot. Antioxidant activity was expressed in H2O2 equivalent (HPEq), representing the volume of PE that corresponds to 1.0 mmol L-1 H2O2 decrease. Resulting HPEq ranged between 1.71 +/- 0.11 and 8.00 +/- 0.18 mu L. Range of 1,1-dipheny1-2-picryl hydrazyl (DPPH) radical scavenging activity was from 0.093 +/- 0.004% to 0.346 +/- 0.006%. Total phenolic content (TCP) of PE with superior AO activity was 5.31 +/- 0.05%g GAE, while the extract with the lowest activity contained 1.45 +/- 0.02%g GAE. Antioxidant activity, determined by polarographic method, was correlated with DPPH scavenging activity (R-2 = 0.991) and TCP (R-2 = 0.985). Validity of obtained results was further confirmed using ANOVA and post hoc Tukey HSD test. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Antioxidant activity of propolis extracts from Serbia: A polarographic approach",
volume = "50",
number = "10",
pages = "3614-3618",
doi = "10.1016/j.fct.2012.07.029"
}
Potkonjak, N. I., Veselinović, D. S., Novaković, M. M., Gorjanovic, S. Z., Pezo, L.,& Sužnjevicć, D. Ž. (2012). Antioxidant activity of propolis extracts from Serbia: A polarographic approach.
Food and Chemical Toxicology, 50(10), 3614-3618.
https://doi.org/10.1016/j.fct.2012.07.029
Potkonjak NI, Veselinović DS, Novaković MM, Gorjanovic SZ, Pezo L, Sužnjevicć DŽ. Antioxidant activity of propolis extracts from Serbia: A polarographic approach. Food and Chemical Toxicology. 2012;50(10):3614-3618
Potkonjak Nebojša I., Veselinović Dragan S., Novaković Miroslav M., Gorjanovic Stanislava Z., Pezo Lato, Sužnjevicć Desanka Ž., "Antioxidant activity of propolis extracts from Serbia: A polarographic approach" Food and Chemical Toxicology, 50, no. 10 (2012):3614-3618,
https://doi.org/10.1016/j.fct.2012.07.029 .
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