TY  - JOUR
AU  - Đurašević, Siniša
AU  - Nikolić, Gorana V.
AU  - Todorović, Ana
AU  - Drakulić, Dunja R.
AU  - Pejić, Snežana
AU  - Martinović, Vesna
AU  - Mitić-Ćulafić, Dragana
AU  - Milić, Dragana
AU  - Kop, Tatjana
AU  - Jasnić, Nebojša
AU  - Đorđević, Jelena D.
AU  - Todorović, Zoran
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8924
AB  - The effects of twelve weeks of supplementation with fullerene C60 olive/coconut oil solution on a broad spectrum of parameters in rats were examined. The tissue bioaccumulation of C60 was shown to be tissue-specific, with the liver, heart, and adrenal glands being the organs of the greatest, and the kidney, brain, and spleen being the organs of the smallest accumulation. C60 did not change aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase serum activities level, nor the damage of liver cells DNA. There were no effects of fullerene on prooxidant-antioxidant balance in the liver, kidney, spleen, heart, and brain, nor any visible harmful effects on the liver, heart, aorta, spleen, kidney, and small intestine histology. Fullerene changed the gut microbiota structure towards the bacteria that ameliorate lipid homeostasis, causing a serum triglycerides concentration decrease. However, C60 significantly increased the insulin resistance, serum ascorbate oxidation, and brain malondialdehyde and advanced oxidation protein products level. The deteriorative effects of C60 on the brain and serum could be attributed to the specific physicochemical composition of these tissues, potentiating the C60 aggregation or biotransformation as the key element of its pro-oxidative action.
T2  - Food and Chemical Toxicology
T1  - Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats
VL  - 140
DO  - 10.1016/j.fct.2020.111302
ER  - 

@article{
author = "Đurašević, Siniša and Nikolić, Gorana V. and Todorović, Ana and Drakulić, Dunja R. and Pejić, Snežana and Martinović, Vesna and Mitić-Ćulafić, Dragana and Milić, Dragana and Kop, Tatjana and Jasnić, Nebojša and Đorđević, Jelena D. and Todorović, Zoran",
year = "2020",
abstract = "The effects of twelve weeks of supplementation with fullerene C60 olive/coconut oil solution on a broad spectrum of parameters in rats were examined. The tissue bioaccumulation of C60 was shown to be tissue-specific, with the liver, heart, and adrenal glands being the organs of the greatest, and the kidney, brain, and spleen being the organs of the smallest accumulation. C60 did not change aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase serum activities level, nor the damage of liver cells DNA. There were no effects of fullerene on prooxidant-antioxidant balance in the liver, kidney, spleen, heart, and brain, nor any visible harmful effects on the liver, heart, aorta, spleen, kidney, and small intestine histology. Fullerene changed the gut microbiota structure towards the bacteria that ameliorate lipid homeostasis, causing a serum triglycerides concentration decrease. However, C60 significantly increased the insulin resistance, serum ascorbate oxidation, and brain malondialdehyde and advanced oxidation protein products level. The deteriorative effects of C60 on the brain and serum could be attributed to the specific physicochemical composition of these tissues, potentiating the C60 aggregation or biotransformation as the key element of its pro-oxidative action.",
journal = "Food and Chemical Toxicology",
title = "Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats",
volume = "140",
doi = "10.1016/j.fct.2020.111302"
}

Đurašević, S., Nikolić, G. V., Todorović, A., Drakulić, D. R., Pejić, S., Martinović, V., Mitić-Ćulafić, D., Milić, D., Kop, T., Jasnić, N., Đorđević, J. D.,& Todorović, Z.. (2020). Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats. in Food and Chemical Toxicology, 140.
https://doi.org/10.1016/j.fct.2020.111302

Đurašević S, Nikolić GV, Todorović A, Drakulić DR, Pejić S, Martinović V, Mitić-Ćulafić D, Milić D, Kop T, Jasnić N, Đorđević JD, Todorović Z. Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats. in Food and Chemical Toxicology. 2020;140.
doi:10.1016/j.fct.2020.111302 .

Đurašević, Siniša, Nikolić, Gorana V., Todorović, Ana, Drakulić, Dunja R., Pejić, Snežana, Martinović, Vesna, Mitić-Ćulafić, Dragana, Milić, Dragana, Kop, Tatjana, Jasnić, Nebojša, Đorđević, Jelena D., Todorović, Zoran, "Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats" in Food and Chemical Toxicology, 140 (2020),
https://doi.org/10.1016/j.fct.2020.111302 . .

TY  - JOUR
AU  - Jovanović, Predrag
AU  - Spasojević, Nataša
AU  - Puškaš, Nela
AU  - Stefanović, Bojana
AU  - Dronjak, Slađana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8400
AB  - Social stress produces behavioral alterations, and autonomic and cardiac dysfunction in animals. In addition to the well-known roles of oxytocin on birth and maternal bonding, recent evidence shows that this neuropeptide possesses cardio-protective properties. However less is known about its role in the regulation of the autonomic nervous system. The direct influence of oxytocin on the cardiac catecholamine synthesizing enzyme, transport beta-adrenoceptors and muscarinic receptors in animals exposed to chronic social isolation stress has not yet been studied. In this study, we examined the influence of peripheral chronic oxytocin treatment on anxiety-related behavior, the morphology and content of epinephrine and norepinephrine, mRNA and protein levels of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and receptors <beta> 3 (β3-AR) and muscarinic 2 (M2 MR) in the right and left cardiac atrium and ventricle of chronically socially isolated male rats. Our results show that oxytocin treatment exhibits an anxiolytic effect, decreases the heart/body weight ratio and prevents the hypertrophy of cardiomyocytes in the wall of the left ventricle of stressed rats. Epinephrine and TH protein levels were unchanged after prolonged oxytocin treatment. Peripheral oxytocin administration led to the enhancement of gene expression of β3-AR in both atria, NET protein in the left ventricle and gene expression of M2 MR in the right atrium and the left ventricle of chronically socially isolated rats. The study provides evidence that oxytocin treatment in chronically socially isolated animals enhances norepinephrine uptake and expression of cardio-inhibitory receptors in cardiac tissues, which could have a beneficial effect on the cardiovascular system under the increased activity of the sympathoneural system. © 2018 Elsevier Inc.
T2  - Peptides
T1  - Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats
VL  - 111
SP  - 132
EP  - 141
DO  - 10.1016/j.peptides.2018.06.008
ER  - 

@article{
author = "Jovanović, Predrag and Spasojević, Nataša and Puškaš, Nela and Stefanović, Bojana and Dronjak, Slađana",
year = "2019",
abstract = "Social stress produces behavioral alterations, and autonomic and cardiac dysfunction in animals. In addition to the well-known roles of oxytocin on birth and maternal bonding, recent evidence shows that this neuropeptide possesses cardio-protective properties. However less is known about its role in the regulation of the autonomic nervous system. The direct influence of oxytocin on the cardiac catecholamine synthesizing enzyme, transport beta-adrenoceptors and muscarinic receptors in animals exposed to chronic social isolation stress has not yet been studied. In this study, we examined the influence of peripheral chronic oxytocin treatment on anxiety-related behavior, the morphology and content of epinephrine and norepinephrine, mRNA and protein levels of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and receptors <beta> 3 (β3-AR) and muscarinic 2 (M2 MR) in the right and left cardiac atrium and ventricle of chronically socially isolated male rats. Our results show that oxytocin treatment exhibits an anxiolytic effect, decreases the heart/body weight ratio and prevents the hypertrophy of cardiomyocytes in the wall of the left ventricle of stressed rats. Epinephrine and TH protein levels were unchanged after prolonged oxytocin treatment. Peripheral oxytocin administration led to the enhancement of gene expression of β3-AR in both atria, NET protein in the left ventricle and gene expression of M2 MR in the right atrium and the left ventricle of chronically socially isolated rats. The study provides evidence that oxytocin treatment in chronically socially isolated animals enhances norepinephrine uptake and expression of cardio-inhibitory receptors in cardiac tissues, which could have a beneficial effect on the cardiovascular system under the increased activity of the sympathoneural system. © 2018 Elsevier Inc.",
journal = "Peptides",
title = "Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats",
volume = "111",
pages = "132-141",
doi = "10.1016/j.peptides.2018.06.008"
}

Jovanović, P., Spasojević, N., Puškaš, N., Stefanović, B.,& Dronjak, S.. (2019). Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats. in Peptides, 111, 132-141.
https://doi.org/10.1016/j.peptides.2018.06.008

Jovanović P, Spasojević N, Puškaš N, Stefanović B, Dronjak S. Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats. in Peptides. 2019;111:132-141.
doi:10.1016/j.peptides.2018.06.008 .

Jovanović, Predrag, Spasojević, Nataša, Puškaš, Nela, Stefanović, Bojana, Dronjak, Slađana, "Oxytocin modulates the expression of norepinephrine transporter, β3-adrenoceptors and muscarinic M2 receptors in the hearts of socially isolated rats" in Peptides, 111 (2019):132-141,
https://doi.org/10.1016/j.peptides.2018.06.008 . .

TY  - JOUR
AU  - Zafirović, Sonja
AU  - Sudar-Milovanović, Emina
AU  - Obradović, Milan M.
AU  - Đorđević, Jelena D.
AU  - Jasnić, Nebojša
AU  - Labudović-Borović, Milica
AU  - Isenović, Esma R.
PY  - 2019
UR  - http://www.eurekaselect.com/159734/article
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8097
AB  - BACKGROUND: Oestradiol is an important regulatory factor with several positive effects on the cardiovascular (CV) system. We evaluated the molecular mechanism of the in vivo effects of oestradiol on the regulation of cardiac inducible nitric oxide (NO) synthase (iNOS) expression and activity. METHODS: Male Wistar rats were treated with oestradiol (40 mg/kg, intraperitoneally) and after 24 h the animals were sacrificed. The concentrations of NO and L-Arginine (L-Arg) were determined spectrophotometrically. For protein expressions of iNOS, p65 subunit of nuclear factor-κB (NFκB-p65), Ras homolog gene family-member A (RhoA), angiotensin II receptor type 1 (AT1R), insulin receptor substrate 1 (IRS-1), p85, p110 and protein kinase B (Akt), Western blot method was used. Coimmunoprecipitation was used for measuring the association of IRS-1 with the p85 subunit of phosphatidylinositol- 3-kinase (PI3K). The expression of iNOS messenger ribonucleic acid (mRNA) was measured with the quantitative real-time polymerase chain reaction (qRT-PCR). Immunohistochemical analysis of the tissue was used to detect localization and expression of iNOS in heart tissue. RESULTS: Oestradiol treatment reduced L-Arg concentration (p<0.01), iNOS mRNA (p<0.01) and protein (p<0.001) expression, level of RhoA (p<0.05) and AT1R (p<0.001) protein. In contrast, plasma NO (p<0.05), Akt phosphorylation at Thr308 (p<0.05) and protein level of p85 (p<0.001) increased after oestradiol treatment. CONCLUSION: Our results suggest that oestradiol in vivo regulates cardiac iNOS expression via the PI3K/Akt signaling pathway, through attenuation of RhoA and AT1R.
T2  - Current Vascular Pharmacology
T1  - Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression
VL  - 17
IS  - 3
SP  - 307
EP  - 318
DO  - 10.2174/1570161116666180212142414
ER  - 

@article{
author = "Zafirović, Sonja and Sudar-Milovanović, Emina and Obradović, Milan M. and Đorđević, Jelena D. and Jasnić, Nebojša and Labudović-Borović, Milica and Isenović, Esma R.",
year = "2019",
abstract = "BACKGROUND: Oestradiol is an important regulatory factor with several positive effects on the cardiovascular (CV) system. We evaluated the molecular mechanism of the in vivo effects of oestradiol on the regulation of cardiac inducible nitric oxide (NO) synthase (iNOS) expression and activity. METHODS: Male Wistar rats were treated with oestradiol (40 mg/kg, intraperitoneally) and after 24 h the animals were sacrificed. The concentrations of NO and L-Arginine (L-Arg) were determined spectrophotometrically. For protein expressions of iNOS, p65 subunit of nuclear factor-κB (NFκB-p65), Ras homolog gene family-member A (RhoA), angiotensin II receptor type 1 (AT1R), insulin receptor substrate 1 (IRS-1), p85, p110 and protein kinase B (Akt), Western blot method was used. Coimmunoprecipitation was used for measuring the association of IRS-1 with the p85 subunit of phosphatidylinositol- 3-kinase (PI3K). The expression of iNOS messenger ribonucleic acid (mRNA) was measured with the quantitative real-time polymerase chain reaction (qRT-PCR). Immunohistochemical analysis of the tissue was used to detect localization and expression of iNOS in heart tissue. RESULTS: Oestradiol treatment reduced L-Arg concentration (p<0.01), iNOS mRNA (p<0.01) and protein (p<0.001) expression, level of RhoA (p<0.05) and AT1R (p<0.001) protein. In contrast, plasma NO (p<0.05), Akt phosphorylation at Thr308 (p<0.05) and protein level of p85 (p<0.001) increased after oestradiol treatment. CONCLUSION: Our results suggest that oestradiol in vivo regulates cardiac iNOS expression via the PI3K/Akt signaling pathway, through attenuation of RhoA and AT1R.",
journal = "Current Vascular Pharmacology",
title = "Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression",
volume = "17",
number = "3",
pages = "307-318",
doi = "10.2174/1570161116666180212142414"
}

Zafirović, S., Sudar-Milovanović, E., Obradović, M. M., Đorđević, J. D., Jasnić, N., Labudović-Borović, M.,& Isenović, E. R.. (2019). Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression. in Current Vascular Pharmacology, 17(3), 307-318.
https://doi.org/10.2174/1570161116666180212142414

Zafirović S, Sudar-Milovanović E, Obradović MM, Đorđević JD, Jasnić N, Labudović-Borović M, Isenović ER. Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression. in Current Vascular Pharmacology. 2019;17(3):307-318.
doi:10.2174/1570161116666180212142414 .

Zafirović, Sonja, Sudar-Milovanović, Emina, Obradović, Milan M., Đorđević, Jelena D., Jasnić, Nebojša, Labudović-Borović, Milica, Isenović, Esma R., "Involvement of PI3K, Akt and RhoA in Oestradiol Regulation of Cardiac iNOS Expression" in Current Vascular Pharmacology, 17, no. 3 (2019):307-318,
https://doi.org/10.2174/1570161116666180212142414 . .

TY  - JOUR
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7584
AB  - The dysfunction of parvalbumin-positive (PV+) interneurons, the most abundant type of hippocampal GABAergic inhibitory interneuron, has been implicated in mood disorders. We recently reported that adult male Wistar rats exposed to three weeks of social isolation show depressive-and anxiety-like behaviors and a reduced number of PV+ interneurons in all hippocampal subregions. As GABA neurotransmission has been proposed as a potential therapeutic target of antidepressant and antipsychotic medications, we examined whether treatment with the antidepressant fluoxetine (Flx) (15 mg/kg/day) or the antipsychotic clozapine (Clz) (20 mg/kg/day) during three weeks of social isolation in rats offered protection from the isolation stress-induced reduction in the number of PV+ interneurons in hippocampal subregions. Using immunofluorescence analysis, we revealed that both chronic Flx and Clz partially prevented the isolation-induced changes. Flx prevented the reduction in the number of PV+ interneurons in the CA2, CA3, without affecting the CA1 and dentate gyrus DG areas, whereas Clz prevented this decrement in the CA2, CA3 and DG regions but not in CA1 areas. Moreover, Flx increased the number of PV+ interneurons in CA1 in control animals. These findings suggest that chronic administration of Flx or Clz may offer partial protection from social isolation stress via modulation of the hippocampal GABAergic system. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved.
T2  - Neuroscience
T1  - Chronic Treatment with Fluoxetine or Clozapine of Socially Isolated Rats Prevents Subsector-Specific Reduction of Parvalbumin Immunoreactive Cells in the Hippocampus
VL  - 371
SP  - 384
EP  - 394
DO  - 10.1016/j.neuroscience.2017.12.020
ER  - 

@article{
year = "2018",
abstract = "The dysfunction of parvalbumin-positive (PV+) interneurons, the most abundant type of hippocampal GABAergic inhibitory interneuron, has been implicated in mood disorders. We recently reported that adult male Wistar rats exposed to three weeks of social isolation show depressive-and anxiety-like behaviors and a reduced number of PV+ interneurons in all hippocampal subregions. As GABA neurotransmission has been proposed as a potential therapeutic target of antidepressant and antipsychotic medications, we examined whether treatment with the antidepressant fluoxetine (Flx) (15 mg/kg/day) or the antipsychotic clozapine (Clz) (20 mg/kg/day) during three weeks of social isolation in rats offered protection from the isolation stress-induced reduction in the number of PV+ interneurons in hippocampal subregions. Using immunofluorescence analysis, we revealed that both chronic Flx and Clz partially prevented the isolation-induced changes. Flx prevented the reduction in the number of PV+ interneurons in the CA2, CA3, without affecting the CA1 and dentate gyrus DG areas, whereas Clz prevented this decrement in the CA2, CA3 and DG regions but not in CA1 areas. Moreover, Flx increased the number of PV+ interneurons in CA1 in control animals. These findings suggest that chronic administration of Flx or Clz may offer partial protection from social isolation stress via modulation of the hippocampal GABAergic system. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved.",
journal = "Neuroscience",
title = "Chronic Treatment with Fluoxetine or Clozapine of Socially Isolated Rats Prevents Subsector-Specific Reduction of Parvalbumin Immunoreactive Cells in the Hippocampus",
volume = "371",
pages = "384-394",
doi = "10.1016/j.neuroscience.2017.12.020"
}

(2018). Chronic Treatment with Fluoxetine or Clozapine of Socially Isolated Rats Prevents Subsector-Specific Reduction of Parvalbumin Immunoreactive Cells in the Hippocampus. in Neuroscience, 371, 384-394.
https://doi.org/10.1016/j.neuroscience.2017.12.020

Chronic Treatment with Fluoxetine or Clozapine of Socially Isolated Rats Prevents Subsector-Specific Reduction of Parvalbumin Immunoreactive Cells in the Hippocampus. in Neuroscience. 2018;371:384-394.
doi:10.1016/j.neuroscience.2017.12.020 .

"Chronic Treatment with Fluoxetine or Clozapine of Socially Isolated Rats Prevents Subsector-Specific Reduction of Parvalbumin Immunoreactive Cells in the Hippocampus" in Neuroscience, 371 (2018):384-394,
https://doi.org/10.1016/j.neuroscience.2017.12.020 . .

TY  - JOUR
AU  - Filipović, Dragana
AU  - Todorović, Nevena
AU  - Bernardi, Rick E.
AU  - Gass, Peter
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1392
AB  - Various stressors may disrupt the redox homeostasis of an organism by causing oxidative and nitrosative stress that may activate stressor-specific pathways and provoke specific responses. Chronic social isolation (CSIS) represents a mild chronic stress that evokes a variety of neurobehavioral changes in rats similar to those observed in people with psychiatric disorders, including depression. Most rodent studies have focused on the effect of social isolation during weaning or adolescence, while its effect in adult rats has not been extensively examined. In this review, we discuss the current knowledge regarding the involvement of oxidative/nitrosative stress pathways in the prefrontal cortex and hippocampus of adult male rats exposed to CSIS, focusing on hypothalamic-pituitary-adrenocortical (HPA) axis activity, behavior parameters, antioxidative defense systems, stress signaling mediated by nuclear factor-kappa B (NF-kappa B), and mitochondria-related proapoptotic signaling. Although increased concentrations of corticosterone (CORT) have been shown to induce oxidative and nitrosative stress, we suggest a mechanism underlying the glucocorticoid paradox whereby a state of oxidative/nitrosative stress may exist under basal CORT levels. This review also highlights the differential susceptibility of prefrontal cortex and hippocampus to oxidative stress following CSIS and suggests a possible cellular pathway of stress tolerance that preserves the hippocampus from molecular damage and apoptosis. The differential regulation of the transcriptional factor NF-kappa B, and the enzymes inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) following CSIS may be one functional difference between the response of the prefrontal cortex and hippocampus, thus identifying potentially relevant targets for antidepressant treatment.
T2  - Brain Structure and Function
T1  - Oxidative and nitrosative stress pathways in the brain of socially isolated adult male rats demonstrating depressive- and anxiety-like symptoms
VL  - 222
IS  - 1
SP  - 1
EP  - 20
DO  - 10.1007/s00429-016-1218-9
ER  - 

@article{
author = "Filipović, Dragana and Todorović, Nevena and Bernardi, Rick E. and Gass, Peter",
year = "2017",
abstract = "Various stressors may disrupt the redox homeostasis of an organism by causing oxidative and nitrosative stress that may activate stressor-specific pathways and provoke specific responses. Chronic social isolation (CSIS) represents a mild chronic stress that evokes a variety of neurobehavioral changes in rats similar to those observed in people with psychiatric disorders, including depression. Most rodent studies have focused on the effect of social isolation during weaning or adolescence, while its effect in adult rats has not been extensively examined. In this review, we discuss the current knowledge regarding the involvement of oxidative/nitrosative stress pathways in the prefrontal cortex and hippocampus of adult male rats exposed to CSIS, focusing on hypothalamic-pituitary-adrenocortical (HPA) axis activity, behavior parameters, antioxidative defense systems, stress signaling mediated by nuclear factor-kappa B (NF-kappa B), and mitochondria-related proapoptotic signaling. Although increased concentrations of corticosterone (CORT) have been shown to induce oxidative and nitrosative stress, we suggest a mechanism underlying the glucocorticoid paradox whereby a state of oxidative/nitrosative stress may exist under basal CORT levels. This review also highlights the differential susceptibility of prefrontal cortex and hippocampus to oxidative stress following CSIS and suggests a possible cellular pathway of stress tolerance that preserves the hippocampus from molecular damage and apoptosis. The differential regulation of the transcriptional factor NF-kappa B, and the enzymes inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) following CSIS may be one functional difference between the response of the prefrontal cortex and hippocampus, thus identifying potentially relevant targets for antidepressant treatment.",
journal = "Brain Structure and Function",
title = "Oxidative and nitrosative stress pathways in the brain of socially isolated adult male rats demonstrating depressive- and anxiety-like symptoms",
volume = "222",
number = "1",
pages = "1-20",
doi = "10.1007/s00429-016-1218-9"
}

Filipović, D., Todorović, N., Bernardi, R. E.,& Gass, P.. (2017). Oxidative and nitrosative stress pathways in the brain of socially isolated adult male rats demonstrating depressive- and anxiety-like symptoms. in Brain Structure and Function, 222(1), 1-20.
https://doi.org/10.1007/s00429-016-1218-9

Filipović D, Todorović N, Bernardi RE, Gass P. Oxidative and nitrosative stress pathways in the brain of socially isolated adult male rats demonstrating depressive- and anxiety-like symptoms. in Brain Structure and Function. 2017;222(1):1-20.
doi:10.1007/s00429-016-1218-9 .

Filipović, Dragana, Todorović, Nevena, Bernardi, Rick E., Gass, Peter, "Oxidative and nitrosative stress pathways in the brain of socially isolated adult male rats demonstrating depressive- and anxiety-like symptoms" in Brain Structure and Function, 222, no. 1 (2017):1-20,
https://doi.org/10.1007/s00429-016-1218-9 . .

TY  - JOUR
AU  - Zaletel, Ivan
AU  - Filipović, Dragana
AU  - Puškaš, Nela
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1664
AB  - Exposure of an organism to chronic psychosocial stress may affect brain-derived neurotrophic factor (BDNF) expression that has been implicated in the etiology of psychiatric disorders, such as depression. Given that depression in humans has been linked with social stress, the chronic social stress paradigms for modeling psychiatric disorders in animals have thus been developed. Chronic social isolation in animal models generally causes changes in hypothalamic-pituitary-adrenal axis functioning, associated with anxiety-and depressive-like behaviors. Also, this chronic stress causes downregulation of BDNF protein and mRNA in the hippocampus, a stress-sensitive brain region closely related to the pathophysiology of depression. In this review, we discuss the current knowledge regarding the structure, function, intracellular signaling, inter-individual differences and epigenetic regulation of BDNF in both physiological conditions and depression and changes in corticosterone levels, as a marker of stress response. Since BDNF levels are age dependent in humans and rodents, this review will also highlight the effects of adolescent and adult chronic social isolation models of both genders on the BDNF expression.
T2  - Reviews in the Neurosciences
T1  - Hippocampal BDNF in physiological conditions and social isolation
VL  - 28
IS  - 6
SP  - 675
EP  - 692
DO  - 10.1515/revneuro-2016-0072
ER  - 

@article{
author = "Zaletel, Ivan and Filipović, Dragana and Puškaš, Nela",
year = "2017",
abstract = "Exposure of an organism to chronic psychosocial stress may affect brain-derived neurotrophic factor (BDNF) expression that has been implicated in the etiology of psychiatric disorders, such as depression. Given that depression in humans has been linked with social stress, the chronic social stress paradigms for modeling psychiatric disorders in animals have thus been developed. Chronic social isolation in animal models generally causes changes in hypothalamic-pituitary-adrenal axis functioning, associated with anxiety-and depressive-like behaviors. Also, this chronic stress causes downregulation of BDNF protein and mRNA in the hippocampus, a stress-sensitive brain region closely related to the pathophysiology of depression. In this review, we discuss the current knowledge regarding the structure, function, intracellular signaling, inter-individual differences and epigenetic regulation of BDNF in both physiological conditions and depression and changes in corticosterone levels, as a marker of stress response. Since BDNF levels are age dependent in humans and rodents, this review will also highlight the effects of adolescent and adult chronic social isolation models of both genders on the BDNF expression.",
journal = "Reviews in the Neurosciences",
title = "Hippocampal BDNF in physiological conditions and social isolation",
volume = "28",
number = "6",
pages = "675-692",
doi = "10.1515/revneuro-2016-0072"
}

Zaletel, I., Filipović, D.,& Puškaš, N.. (2017). Hippocampal BDNF in physiological conditions and social isolation. in Reviews in the Neurosciences, 28(6), 675-692.
https://doi.org/10.1515/revneuro-2016-0072

Zaletel I, Filipović D, Puškaš N. Hippocampal BDNF in physiological conditions and social isolation. in Reviews in the Neurosciences. 2017;28(6):675-692.
doi:10.1515/revneuro-2016-0072 .

Zaletel, Ivan, Filipović, Dragana, Puškaš, Nela, "Hippocampal BDNF in physiological conditions and social isolation" in Reviews in the Neurosciences, 28, no. 6 (2017):675-692,
https://doi.org/10.1515/revneuro-2016-0072 . .

TY  - JOUR
AU  - Stefanović, Bojana
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Jasnić, Nebojša
AU  - Đorđević, Jelena D.
AU  - Dronjak, Slađana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1265
AB  - The hippocampus is sensitive to stress which activates norepinephrine terminals deriving from the locus coeruleus. Melatonin exerts positive effects on the hippocampal neurogenic process and on depressive-like behaviour. Thus, in the present study, an examination was made of the effect of chronic melatonin treatment on norepinephrine content, synthesis, uptake, vesicular transport and degradation in the hippocampus of rats exposed to CUMS. This entailed quantifying the norephinephrine, mRNA and protein levels of DBH, NET, VMAT 2, MAO-A and COMT. The results show that CUMS evoked prolonged immobility. Melatonin treatment decreased immobility in comparison with the placebo group, reflecting an antidepressant-like effect. Compared with the placebo group, a dramatic decrease in norepinephrine content, decreased VMAT2 mRNA and protein and increased MAO-A protein levels in the hippocampus of the CUMS rats were observed. However, no significant differences in the levels of DBH, NET, COMT mRNA and protein and MAO-A mRNA levels between the placebo and the stressed groups were found. The results showed the restorative effects of melatonin on the stress-induced decline in the norepinephrine content of the hippocampus. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in VMAT2 mRNA and protein levels, whereas it reduced the increase of the mRNA of COMT and protein levels of MAO-A. Chronic treatment with melatonin failed to alter the gene expression of DBH or NET in the hippocampus of the CUMS rats. Additionally, the results show that melatonin enhances VMAT2 expression and norepinephrine storage, whilst it reduces norepinephrine degrading enzymes. (C) 2016 Elsevier B.V. and ECNP. All rights reserved.
T2  - European Neuropsychopharmacology
T1  - Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels
VL  - 26
IS  - 10
SP  - 1629
EP  - 1637
DO  - 10.1016/j.euroneuro.2016.07.005
ER  - 

@article{
author = "Stefanović, Bojana and Spasojević, Nataša and Jovanović, Predrag and Jasnić, Nebojša and Đorđević, Jelena D. and Dronjak, Slađana",
year = "2016",
abstract = "The hippocampus is sensitive to stress which activates norepinephrine terminals deriving from the locus coeruleus. Melatonin exerts positive effects on the hippocampal neurogenic process and on depressive-like behaviour. Thus, in the present study, an examination was made of the effect of chronic melatonin treatment on norepinephrine content, synthesis, uptake, vesicular transport and degradation in the hippocampus of rats exposed to CUMS. This entailed quantifying the norephinephrine, mRNA and protein levels of DBH, NET, VMAT 2, MAO-A and COMT. The results show that CUMS evoked prolonged immobility. Melatonin treatment decreased immobility in comparison with the placebo group, reflecting an antidepressant-like effect. Compared with the placebo group, a dramatic decrease in norepinephrine content, decreased VMAT2 mRNA and protein and increased MAO-A protein levels in the hippocampus of the CUMS rats were observed. However, no significant differences in the levels of DBH, NET, COMT mRNA and protein and MAO-A mRNA levels between the placebo and the stressed groups were found. The results showed the restorative effects of melatonin on the stress-induced decline in the norepinephrine content of the hippocampus. It was observed that melatonin treatment in the CUMS rats prevented the stress-induced decrease in VMAT2 mRNA and protein levels, whereas it reduced the increase of the mRNA of COMT and protein levels of MAO-A. Chronic treatment with melatonin failed to alter the gene expression of DBH or NET in the hippocampus of the CUMS rats. Additionally, the results show that melatonin enhances VMAT2 expression and norepinephrine storage, whilst it reduces norepinephrine degrading enzymes. (C) 2016 Elsevier B.V. and ECNP. All rights reserved.",
journal = "European Neuropsychopharmacology",
title = "Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels",
volume = "26",
number = "10",
pages = "1629-1637",
doi = "10.1016/j.euroneuro.2016.07.005"
}

Stefanović, B., Spasojević, N., Jovanović, P., Jasnić, N., Đorđević, J. D.,& Dronjak, S.. (2016). Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels. in European Neuropsychopharmacology, 26(10), 1629-1637.
https://doi.org/10.1016/j.euroneuro.2016.07.005

Stefanović B, Spasojević N, Jovanović P, Jasnić N, Đorđević JD, Dronjak S. Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels. in European Neuropsychopharmacology. 2016;26(10):1629-1637.
doi:10.1016/j.euroneuro.2016.07.005 .

Stefanović, Bojana, Spasojević, Nataša, Jovanović, Predrag, Jasnić, Nebojša, Đorđević, Jelena D., Dronjak, Slađana, "Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels" in European Neuropsychopharmacology, 26, no. 10 (2016):1629-1637,
https://doi.org/10.1016/j.euroneuro.2016.07.005 . .

TY  - JOUR
AU  - Vujović, Predrag
AU  - Lakić, Iva
AU  - Jasnić, Nebojša
AU  - Jevđović, Tanja
AU  - Đurašević, Siniša F.
AU  - Isenović, Esma R.
AU  - Đorđević, Jelena
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1120
AB  - Given that both prolactin and galanin take part in the regulation of energy homeostasis and that galanin is localized within lactotrophs, this study was aimed at comparing the pituitary expression patterns of prolactin and galanin during different phases of metabolic response to starvation in adult Wistar male rats. Food was removed at the onset of the dark phase (6:00 pm) and the animals were deprived for 6, 12, 24 and 48 h. Each of the starved groups (n=6) was killed simultaneously with a group of ad libitum-fed rats (n=6), and the intrapituitary levels of prolactin and galanin were examined. Galanin expression in the hypothalamus and the circulating levels of prolactin were also assessed. Starvation induced a rise in the intrapituitary prolactin level (p LT 0.001), whereas the opposite trend was detected in the serum (p LT 0.05). The galanin pituitary level was initially increased (6, 12 h) (p LT 0.05), but as starvation progressed, it first reached (at 24 h) and ultimately fell below the level recorded in the ad libitum rats (at 48 h) (p LT 0.05). Both prolactin and galanin were elevated in the hypothalamus after 24- and 48-h starvation. The results show that the starvation-induced increase in the pituitary prolactin expression did not lead to the rise in prolactin circulating levels, but rather resulted in the elevation of the prolactin hypothalamic content. Furthermore, the results suggest that under the circumstances of disturbed energy homeostasis, galanin might be responsible for the augmented prolactin production, initially at the pituitary and subsequently at the hypothalamic level.
T2  - Archives of Biological Sciences
T1  - Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern
VL  - 68
IS  - 1
SP  - 117
EP  - 123
DO  - 10.2298/ABS150525133V
ER  - 

@article{
author = "Vujović, Predrag and Lakić, Iva and Jasnić, Nebojša and Jevđović, Tanja and Đurašević, Siniša F. and Isenović, Esma R. and Đorđević, Jelena",
year = "2016",
abstract = "Given that both prolactin and galanin take part in the regulation of energy homeostasis and that galanin is localized within lactotrophs, this study was aimed at comparing the pituitary expression patterns of prolactin and galanin during different phases of metabolic response to starvation in adult Wistar male rats. Food was removed at the onset of the dark phase (6:00 pm) and the animals were deprived for 6, 12, 24 and 48 h. Each of the starved groups (n=6) was killed simultaneously with a group of ad libitum-fed rats (n=6), and the intrapituitary levels of prolactin and galanin were examined. Galanin expression in the hypothalamus and the circulating levels of prolactin were also assessed. Starvation induced a rise in the intrapituitary prolactin level (p LT 0.001), whereas the opposite trend was detected in the serum (p LT 0.05). The galanin pituitary level was initially increased (6, 12 h) (p LT 0.05), but as starvation progressed, it first reached (at 24 h) and ultimately fell below the level recorded in the ad libitum rats (at 48 h) (p LT 0.05). Both prolactin and galanin were elevated in the hypothalamus after 24- and 48-h starvation. The results show that the starvation-induced increase in the pituitary prolactin expression did not lead to the rise in prolactin circulating levels, but rather resulted in the elevation of the prolactin hypothalamic content. Furthermore, the results suggest that under the circumstances of disturbed energy homeostasis, galanin might be responsible for the augmented prolactin production, initially at the pituitary and subsequently at the hypothalamic level.",
journal = "Archives of Biological Sciences",
title = "Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern",
volume = "68",
number = "1",
pages = "117-123",
doi = "10.2298/ABS150525133V"
}

Vujović, P., Lakić, I., Jasnić, N., Jevđović, T., Đurašević, S. F., Isenović, E. R.,& Đorđević, J.. (2016). Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern. in Archives of Biological Sciences, 68(1), 117-123.
https://doi.org/10.2298/ABS150525133V

Vujović P, Lakić I, Jasnić N, Jevđović T, Đurašević SF, Isenović ER, Đorđević J. Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern. in Archives of Biological Sciences. 2016;68(1):117-123.
doi:10.2298/ABS150525133V .

Vujović, Predrag, Lakić, Iva, Jasnić, Nebojša, Jevđović, Tanja, Đurašević, Siniša F., Isenović, Esma R., Đorđević, Jelena, "Time-Dependent Effects of Starvation on Pituitary, Hypothalamic and Serum Prolactin Levels in Rats: Comparison to the Galanin Expression Pattern" in Archives of Biological Sciences, 68, no. 1 (2016):117-123,
https://doi.org/10.2298/ABS150525133V . .

TY  - JOUR
AU  - Jovanović, Predrag
AU  - Spasojević, Nataša
AU  - Stefanović, Bojana
AU  - Božović, N.
AU  - Jasnic, N.
AU  - Đorđević, Jelena D.
AU  - Dronjak, Slađana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5825
AB  - The neuropeptide oxytocin has been shown to influence on neuroendocrine function. The aim of the present study was to investigate the effect of peripheral oxytocin treatment on the synthesis, uptake and content of adreno-medullary catecholamine. For this purpose oxytocin (3.6 mu g/100 g body weight, s.c) was administrated to male rats once a day over 14 days. In order to assess the effect of peripheral oxytocin treatment on adreno-medullary catecholamine we measured epinephrine and norepinephrine content and gene expression of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. Our results show a significant increase of epinephrine (1.7-fold, p LT 0.05) and norepinephrine (1.5-fold, p LT 0.05) content in oxytocin treated animals compared to saline treated ones. Oxytocin treatment had no effect either on mRNA or protein level of TH and NET. Under oxytocin treatment the increase in VMAT2 mRNA level was not statistically significant, but it caused a significant increase in protein level of VMAT2 (3.7-fold, p LT 0.001). These findings indicate that oxytocin treatment increases catecholamine content in the rat adrenal medulla modulating VMAT2 expression. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Peptides
T1  - Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2
VL  - 51
SP  - 110
EP  - 114
DO  - 10.1016/j.peptides.2013.11.001
ER  - 

@article{
author = "Jovanović, Predrag and Spasojević, Nataša and Stefanović, Bojana and Božović, N. and Jasnic, N. and Đorđević, Jelena D. and Dronjak, Slađana",
year = "2014",
abstract = "The neuropeptide oxytocin has been shown to influence on neuroendocrine function. The aim of the present study was to investigate the effect of peripheral oxytocin treatment on the synthesis, uptake and content of adreno-medullary catecholamine. For this purpose oxytocin (3.6 mu g/100 g body weight, s.c) was administrated to male rats once a day over 14 days. In order to assess the effect of peripheral oxytocin treatment on adreno-medullary catecholamine we measured epinephrine and norepinephrine content and gene expression of tyrosine hydroxylase (TH), norepinephrine transporter (NET) and vesicular monoamine transporter 2 (VMAT2) in the adrenal medulla. Our results show a significant increase of epinephrine (1.7-fold, p LT 0.05) and norepinephrine (1.5-fold, p LT 0.05) content in oxytocin treated animals compared to saline treated ones. Oxytocin treatment had no effect either on mRNA or protein level of TH and NET. Under oxytocin treatment the increase in VMAT2 mRNA level was not statistically significant, but it caused a significant increase in protein level of VMAT2 (3.7-fold, p LT 0.001). These findings indicate that oxytocin treatment increases catecholamine content in the rat adrenal medulla modulating VMAT2 expression. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Peptides",
title = "Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2",
volume = "51",
pages = "110-114",
doi = "10.1016/j.peptides.2013.11.001"
}

Jovanović, P., Spasojević, N., Stefanović, B., Božović, N., Jasnic, N., Đorđević, J. D.,& Dronjak, S.. (2014). Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2. in Peptides, 51, 110-114.
https://doi.org/10.1016/j.peptides.2013.11.001

Jovanović P, Spasojević N, Stefanović B, Božović N, Jasnic N, Đorđević JD, Dronjak S. Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2. in Peptides. 2014;51:110-114.
doi:10.1016/j.peptides.2013.11.001 .

Jovanović, Predrag, Spasojević, Nataša, Stefanović, Bojana, Božović, N., Jasnic, N., Đorđević, Jelena D., Dronjak, Slađana, "Peripheral oxytocin treatment affects the rat adreno-medullary catecholamine content modulating expression of vesicular monoamine transporter 2" in Peptides, 51 (2014):110-114,
https://doi.org/10.1016/j.peptides.2013.11.001 . .

TY  - CONF
AU  - Todorović, Nevena
AU  - Stevanović, Jelena
AU  - Filipović, Dragana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9267
AB  - Chronic psychosocial stress is a risk factor for depression. As the liver is a
primary organ for drug activation and detoxification, the effect of chronic
administration of olanzapine on rat hepatic glutathione (GSH)-dependent
defense system including GSH, glutathione peroxidase (GPx) and
glutathione reductase (GLR), following 21d of chronic social isolation stress
(CSIS), an animal model of depression, was investigated. Increased GSH
content in olanzapine-treated controls was found. Protein levels of GPx
were increased in both vehicle- and olanzapine-treated chronically-isolated
animals while significant increase in GPx activity was seen only in
olanzapine-treated chronically-isolated animals. Reduced activity of GLR in
CSIS group that was not in correspondence with its protein level was
increased by olanzapine. The results imply that chronic administration of
olanzapine reverts the alternations in hepatic GSH-dependent defense of
stressed rats caused by CSIS.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Olanzapine reverts the isolation-induced alterations in rat hepatic glutathione-dependent defense system
VL  - Q-05-P
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9267
ER  - 

@conference{
author = "Todorović, Nevena and Stevanović, Jelena and Filipović, Dragana",
year = "2014",
abstract = "Chronic psychosocial stress is a risk factor for depression. As the liver is a
primary organ for drug activation and detoxification, the effect of chronic
administration of olanzapine on rat hepatic glutathione (GSH)-dependent
defense system including GSH, glutathione peroxidase (GPx) and
glutathione reductase (GLR), following 21d of chronic social isolation stress
(CSIS), an animal model of depression, was investigated. Increased GSH
content in olanzapine-treated controls was found. Protein levels of GPx
were increased in both vehicle- and olanzapine-treated chronically-isolated
animals while significant increase in GPx activity was seen only in
olanzapine-treated chronically-isolated animals. Reduced activity of GLR in
CSIS group that was not in correspondence with its protein level was
increased by olanzapine. The results imply that chronic administration of
olanzapine reverts the alternations in hepatic GSH-dependent defense of
stressed rats caused by CSIS.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Olanzapine reverts the isolation-induced alterations in rat hepatic glutathione-dependent defense system",
volume = "Q-05-P",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9267"
}

Todorović, N., Stevanović, J.,& Filipović, D.. (2014). Olanzapine reverts the isolation-induced alterations in rat hepatic glutathione-dependent defense system. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., Q-05-P.
https://hdl.handle.net/21.15107/rcub_vinar_9267

Todorović N, Stevanović J, Filipović D. Olanzapine reverts the isolation-induced alterations in rat hepatic glutathione-dependent defense system. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2014;Q-05-P.
https://hdl.handle.net/21.15107/rcub_vinar_9267 .

Todorović, Nevena, Stevanović, Jelena, Filipović, Dragana, "Olanzapine reverts the isolation-induced alterations in rat hepatic glutathione-dependent defense system" in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry, Q-05-P (2014),
https://hdl.handle.net/21.15107/rcub_vinar_9267 .

TY  - CONF
AU  - Bošković, Maja
AU  - Todorović, Nevena
AU  - Filipović, Dragana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9266
AB  - Changes in glutathione (GSH)-related systems are implicated in depressive
disorders. Since chronic psychosocial stress contributes to depression, we
investigated the effects of 21d of chronic social isolation (CSIS) stress, an
animal model of depression, as well as chronic administration of clozapine,
an atypical antipsychotic, on GSH content, glutathione peroxidase (GPx)
and glutathione reductase (GLR) in the prefrontal cortex of rats. Increased
GPx protein expression and its activity in clozapine-treated (controls or
chronically-isolated) rats as well as in CSIS group were found. Nonetheless,
clozapine administration caused decrease in GSH content but no effects on
GLR in controls and CSIS group. Data indicate that CSIS compromises
GSH-dependent redox system promoting oxidative stress in rat prefrontal
cortex which can’t be protected by clozapine. Moreover, clozapine
administration in controls has a harmful side effect on this redox system.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Compromised glutathione-dependent redox system of chronically-isolated rats: a harmful side effect of clozapine
SP  - 1113
EP  - 1116
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9266
ER  - 

@conference{
author = "Bošković, Maja and Todorović, Nevena and Filipović, Dragana",
year = "2014",
abstract = "Changes in glutathione (GSH)-related systems are implicated in depressive
disorders. Since chronic psychosocial stress contributes to depression, we
investigated the effects of 21d of chronic social isolation (CSIS) stress, an
animal model of depression, as well as chronic administration of clozapine,
an atypical antipsychotic, on GSH content, glutathione peroxidase (GPx)
and glutathione reductase (GLR) in the prefrontal cortex of rats. Increased
GPx protein expression and its activity in clozapine-treated (controls or
chronically-isolated) rats as well as in CSIS group were found. Nonetheless,
clozapine administration caused decrease in GSH content but no effects on
GLR in controls and CSIS group. Data indicate that CSIS compromises
GSH-dependent redox system promoting oxidative stress in rat prefrontal
cortex which can’t be protected by clozapine. Moreover, clozapine
administration in controls has a harmful side effect on this redox system.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Compromised glutathione-dependent redox system of chronically-isolated rats: a harmful side effect of clozapine",
pages = "1113-1116",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9266"
}

Bošković, M., Todorović, N.,& Filipović, D.. (2014). Compromised glutathione-dependent redox system of chronically-isolated rats: a harmful side effect of clozapine. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., 1113-1116.
https://hdl.handle.net/21.15107/rcub_vinar_9266

Bošković M, Todorović N, Filipović D. Compromised glutathione-dependent redox system of chronically-isolated rats: a harmful side effect of clozapine. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2014;:1113-1116.
https://hdl.handle.net/21.15107/rcub_vinar_9266 .

Bošković, Maja, Todorović, Nevena, Filipović, Dragana, "Compromised glutathione-dependent redox system of chronically-isolated rats: a harmful side effect of clozapine" in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry (2014):1113-1116,
https://hdl.handle.net/21.15107/rcub_vinar_9266 .

TY  - CONF
AU  - Todorović, Nevena
AU  - Bošković, Maja
AU  - Filipović, Dragana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9269
AB  - Chronic exposure to psychosocial stress is implicated in the
pathophysiology of depression. We investigated the effect of 21d of chronic
social isolation (CSIS) stress (an animal model of depression) and/or
chronic administration of fluoxetine (15 mg/kg/day), an antidepressant, on
GSH content, protein expression and activity of glutathione peroxidase
(GPx) and glutathione reductase (GLR) in the cytosolic fraction of rat
hippocampus. CSIS stress caused reduced GPx and GLR protein expression
which was not prevented with fluoxetine treatment. Moreover, fluoxetine
administration intensified reduction of these proteins expression. Decreased
GSH content, GPx and GLR activity was also found in chronically-isolated
animals (vehicle- or fluoxetine treated). Data indicate that fluoxetine not
only failed to prevent CSIS-induced changes but itself compromised GSHdependent
defense system in control animals.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Fluoxetine failed to prevent isolation-induced changes of glutathione-dependent defense in rat hippocampus
VL  - Q-06-P
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9269
ER  - 

@conference{
author = "Todorović, Nevena and Bošković, Maja and Filipović, Dragana",
year = "2014",
abstract = "Chronic exposure to psychosocial stress is implicated in the
pathophysiology of depression. We investigated the effect of 21d of chronic
social isolation (CSIS) stress (an animal model of depression) and/or
chronic administration of fluoxetine (15 mg/kg/day), an antidepressant, on
GSH content, protein expression and activity of glutathione peroxidase
(GPx) and glutathione reductase (GLR) in the cytosolic fraction of rat
hippocampus. CSIS stress caused reduced GPx and GLR protein expression
which was not prevented with fluoxetine treatment. Moreover, fluoxetine
administration intensified reduction of these proteins expression. Decreased
GSH content, GPx and GLR activity was also found in chronically-isolated
animals (vehicle- or fluoxetine treated). Data indicate that fluoxetine not
only failed to prevent CSIS-induced changes but itself compromised GSHdependent
defense system in control animals.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Fluoxetine failed to prevent isolation-induced changes of glutathione-dependent defense in rat hippocampus",
volume = "Q-06-P",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9269"
}

Todorović, N., Bošković, M.,& Filipović, D.. (2014). Fluoxetine failed to prevent isolation-induced changes of glutathione-dependent defense in rat hippocampus. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., Q-06-P.
https://hdl.handle.net/21.15107/rcub_vinar_9269

Todorović N, Bošković M, Filipović D. Fluoxetine failed to prevent isolation-induced changes of glutathione-dependent defense in rat hippocampus. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2014;Q-06-P.
https://hdl.handle.net/21.15107/rcub_vinar_9269 .

Todorović, Nevena, Bošković, Maja, Filipović, Dragana, "Fluoxetine failed to prevent isolation-induced changes of glutathione-dependent defense in rat hippocampus" in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry, Q-06-P (2014),
https://hdl.handle.net/21.15107/rcub_vinar_9269 .

TY  - JOUR
AU  - Martinović, Jelena
AU  - Todorović, Nevena
AU  - Tomanović, Nada
AU  - Bošković, Maja
AU  - Djordjevic, Snezana
AU  - Lazarević-Pašti, Tamara
AU  - Bernardi, Rick E.
AU  - Đurđević, Aleksandra
AU  - Filipović, Dragana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6043
AB  - Chronic exposure to stress contributes to the etiology of mood disorders, and the liver as a target organ of antidepressant and antipsychotic drug metabolism is vulnerable to drug-induced toxicity. We investigated the effects of chronic administration of fluoxetine (15 mg/kg/day) or clozapine (20 mg/kg/day) on liver injury via the measurement of liver enzymes, oxidative stress and histopathology in rats exposed to chronic social isolation (21 days), an animal model of depression, and controls. The activity of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the liver content of carbonyl groups, malonyldialdehyde (MDA), reduced glutathione (GSH), cytosolic glutathione S-transferase (GST) and nitric oxide (NO) metabolites were determined. We also characterized nuclear factor-kappa B (NF-kappa B), cyclooxygenase-2 (COX-2) and CuZn-superoxide dismutase (CuZnSOD) protein expression as well as histopathological changes. Increased serum ALT activity in chronically-isolated and control animals treated with both drugs was found while increased AST activity was observed only in fluoxetine-treated rats (chronically-isolated and controls). Increased carbonyl content, MDA, GST activity and decreased GSH levels in drug-treated controls/chronically-isolated animals suggest a link between drugs and hepatic oxidative stress. Increased NO levels associated with NF-kappa B activation and the concomitant increased COX-2 expression together with compromised CuZnSOD expression in clozapine-treated chronically-isolated rats likely reinforce oxidative stress, observed by increased lipid peroxidation and GSH depletion. In contrast, fluoxetine reduced NO levels in chronically-isolated rats. Isolation induced oxidative stress but histological changes were similar to those observed in vehicle-treated controls. Chronic administration of fluoxetine in both chronically-isolated and control animals resulted in more or less normal hepatic architecture, while clozapine in both groups resulted in liver injury. These data suggest that clozapine appears to have a higher potential to induce liver toxicity than fluoxetine. (C) 2014 Elsevier B.V. All rights reserved.
T2  - European Journal of Pharmaceutical Sciences
T1  - Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study
VL  - 59
SP  - 20
EP  - 30
DO  - 10.1016/j.ejps.2014.04.010
ER  - 

@article{
author = "Martinović, Jelena and Todorović, Nevena and Tomanović, Nada and Bošković, Maja and Djordjevic, Snezana and Lazarević-Pašti, Tamara and Bernardi, Rick E. and Đurđević, Aleksandra and Filipović, Dragana",
year = "2014",
abstract = "Chronic exposure to stress contributes to the etiology of mood disorders, and the liver as a target organ of antidepressant and antipsychotic drug metabolism is vulnerable to drug-induced toxicity. We investigated the effects of chronic administration of fluoxetine (15 mg/kg/day) or clozapine (20 mg/kg/day) on liver injury via the measurement of liver enzymes, oxidative stress and histopathology in rats exposed to chronic social isolation (21 days), an animal model of depression, and controls. The activity of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the liver content of carbonyl groups, malonyldialdehyde (MDA), reduced glutathione (GSH), cytosolic glutathione S-transferase (GST) and nitric oxide (NO) metabolites were determined. We also characterized nuclear factor-kappa B (NF-kappa B), cyclooxygenase-2 (COX-2) and CuZn-superoxide dismutase (CuZnSOD) protein expression as well as histopathological changes. Increased serum ALT activity in chronically-isolated and control animals treated with both drugs was found while increased AST activity was observed only in fluoxetine-treated rats (chronically-isolated and controls). Increased carbonyl content, MDA, GST activity and decreased GSH levels in drug-treated controls/chronically-isolated animals suggest a link between drugs and hepatic oxidative stress. Increased NO levels associated with NF-kappa B activation and the concomitant increased COX-2 expression together with compromised CuZnSOD expression in clozapine-treated chronically-isolated rats likely reinforce oxidative stress, observed by increased lipid peroxidation and GSH depletion. In contrast, fluoxetine reduced NO levels in chronically-isolated rats. Isolation induced oxidative stress but histological changes were similar to those observed in vehicle-treated controls. Chronic administration of fluoxetine in both chronically-isolated and control animals resulted in more or less normal hepatic architecture, while clozapine in both groups resulted in liver injury. These data suggest that clozapine appears to have a higher potential to induce liver toxicity than fluoxetine. (C) 2014 Elsevier B.V. All rights reserved.",
journal = "European Journal of Pharmaceutical Sciences",
title = "Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study",
volume = "59",
pages = "20-30",
doi = "10.1016/j.ejps.2014.04.010"
}

Martinović, J., Todorović, N., Tomanović, N., Bošković, M., Djordjevic, S., Lazarević-Pašti, T., Bernardi, R. E., Đurđević, A.,& Filipović, D.. (2014). Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study. in European Journal of Pharmaceutical Sciences, 59, 20-30.
https://doi.org/10.1016/j.ejps.2014.04.010

Martinović J, Todorović N, Tomanović N, Bošković M, Djordjevic S, Lazarević-Pašti T, Bernardi RE, Đurđević A, Filipović D. Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study. in European Journal of Pharmaceutical Sciences. 2014;59:20-30.
doi:10.1016/j.ejps.2014.04.010 .

Martinović, Jelena, Todorović, Nevena, Tomanović, Nada, Bošković, Maja, Djordjevic, Snezana, Lazarević-Pašti, Tamara, Bernardi, Rick E., Đurđević, Aleksandra, Filipović, Dragana, "Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study" in European Journal of Pharmaceutical Sciences, 59 (2014):20-30,
https://doi.org/10.1016/j.ejps.2014.04.010 . .

TY  - JOUR
AU  - Cvijic, Gordana
AU  - Lakić, Iva
AU  - Vujovic, R.
AU  - Jasnic, N.
AU  - Djurasevic, S.
AU  - Dronjak, Slađana
AU  - Đorđević, Jelena D.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5603
AB  - The aim of this study was to examine whether the thermogenic potential of rat interscapular brown adipose tissue (IBAT) changes in response to acute and/or chronic exposure to non-thermal stressors (immobilization and isolation), by measuring the uncoupling protein 1 (UCP-1) content, MAO-A, SOD and CAT activities, as well as the number of IBAT sympathetic noradrenaline-containing nerve fibers. Both acute immobilization (2 h) and chronic isolation (21 days), as well as their combined effects, significantly increased the IBAT UCP-1 content in comparison to non-stressed animals. When applied individually, stressors increased the number of sympathetic fibers in comparison to controls, whereas in combination they decreased it. The activity of IBAT monoamine oxidase-A (MAO-A) decreased under the influence of each stressor independent of its type or duration. SOD activity coincided with MAO-A decrement, whereas CAT activity had an opposite pattern of changes. We conclude that acute and chronic exposure to non-thermal stressors, immobilization and isolation, respectively, affect the metabolic potential of rat IBAT, judging by the increase in UCP-1 content and sympathetic outflow. However, when acute immobilization was applied as a novel stressor to previously chronically isolated animals, an increase in the UCP-1 content was accompanied by a lower IBAT sympathetic outflow, suggesting that IBAT metabolic function under various stress condition is not solely dependent on SNS activity.
T2  - Archives of Biological Sciences
T1  - Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity
VL  - 65
IS  - 3
SP  - 919
EP  - 927
DO  - 10.2298/ABS1303919C
ER  - 

@article{
author = "Cvijic, Gordana and Lakić, Iva and Vujovic, R. and Jasnic, N. and Djurasevic, S. and Dronjak, Slađana and Đorđević, Jelena D.",
year = "2013",
abstract = "The aim of this study was to examine whether the thermogenic potential of rat interscapular brown adipose tissue (IBAT) changes in response to acute and/or chronic exposure to non-thermal stressors (immobilization and isolation), by measuring the uncoupling protein 1 (UCP-1) content, MAO-A, SOD and CAT activities, as well as the number of IBAT sympathetic noradrenaline-containing nerve fibers. Both acute immobilization (2 h) and chronic isolation (21 days), as well as their combined effects, significantly increased the IBAT UCP-1 content in comparison to non-stressed animals. When applied individually, stressors increased the number of sympathetic fibers in comparison to controls, whereas in combination they decreased it. The activity of IBAT monoamine oxidase-A (MAO-A) decreased under the influence of each stressor independent of its type or duration. SOD activity coincided with MAO-A decrement, whereas CAT activity had an opposite pattern of changes. We conclude that acute and chronic exposure to non-thermal stressors, immobilization and isolation, respectively, affect the metabolic potential of rat IBAT, judging by the increase in UCP-1 content and sympathetic outflow. However, when acute immobilization was applied as a novel stressor to previously chronically isolated animals, an increase in the UCP-1 content was accompanied by a lower IBAT sympathetic outflow, suggesting that IBAT metabolic function under various stress condition is not solely dependent on SNS activity.",
journal = "Archives of Biological Sciences",
title = "Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity",
volume = "65",
number = "3",
pages = "919-927",
doi = "10.2298/ABS1303919C"
}

Cvijic, G., Lakić, I., Vujovic, R., Jasnic, N., Djurasevic, S., Dronjak, S.,& Đorđević, J. D.. (2013). Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity. in Archives of Biological Sciences, 65(3), 919-927.
https://doi.org/10.2298/ABS1303919C

Cvijic G, Lakić I, Vujovic R, Jasnic N, Djurasevic S, Dronjak S, Đorđević JD. Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity. in Archives of Biological Sciences. 2013;65(3):919-927.
doi:10.2298/ABS1303919C .

Cvijic, Gordana, Lakić, Iva, Vujovic, R., Jasnic, N., Djurasevic, S., Dronjak, Slađana, Đorđević, Jelena D., "Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity" in Archives of Biological Sciences, 65, no. 3 (2013):919-927,
https://doi.org/10.2298/ABS1303919C . .

TY  - JOUR
AU  - Filipović, Dragana
AU  - Martinović, Jelena
AU  - Gass, Peter
AU  - Inta, Dragos
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5391
AB  - The hippocampus plays a central role in stress-related mood disorders. The effects of acute vs. chronic stress on the integrity of hippocampal circuitry in influencing the vulnerability to, or resiliency against, neuronal injury are poorly understood. Here we investigated whether acute vs. chronic psychosocial isolation stress or a combination of the two (chronic stress followed by acute stress) influences the expression of the interneuronal marker parvalbumin (PV) and the chaperone-inducible heat shock protein 70 (Hsp70i) in different subregions of the hippocampus. Low levels of the Ca2+-binding protein (PV) may increase the vulnerability to neuronal injury, and Hsp70i represents an indicator of intense excitation-induced neuronal stress. Adult male Wistar rats were exposed to 2 h of immobilization (IM) or cold (4 degrees C) (acute stressors), 21 d of social isolation (chronic stress), or a combination of both acute and chronic stress. Both chronic isolation and the combined stressors strongly decreased the PV-Immunoreactive cells in the CA1, CA3 and dentate gyrus (DG) region of the hippocampus, while acute stress did not affect PV expression. The combination of acute and chronic stress induced a dramatic increase in Hsp70i expression in the DG, but Hsp70i expression was unaffected in acute and chronic stress alone. We also monitored serum corticosterone (CORT) levels as a neuroendocrine marker of the stress response. Acute stress increased CORT levels, while chronic isolation stress compromised hypothalamic pituitary adrenocortical (HPA) axis activity such that the normal stress response was impaired following subsequent acute stress. These results indicate that in contrast to acute stress, chronic isolation compromises the HPA axis and generates a considerable reduction in PV expression, representing a decrease in the calcium-buffering capacity and a putatively higher vulnerability of specific hippocampal interneurons to excitotoxic injury. The induction of Hsp70i expression in response to acute and chronic isolation reveals that neurons in the DG are particularly vulnerable to an acute stressor following a chronic perturbation of HPA activity. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.
T2  - Neuroscience
T1  - The Differential Effects of Acute Vs. Chronic Stress and Their Combination on Hippocampal Parvalbumin and Inducible Heat Shock Protein 70 Expression
VL  - 236
SP  - 47
EP  - 54
DO  - 10.1016/j.neuroscience.2013.01.033
ER  - 

@article{
author = "Filipović, Dragana and Martinović, Jelena and Gass, Peter and Inta, Dragos",
year = "2013",
abstract = "The hippocampus plays a central role in stress-related mood disorders. The effects of acute vs. chronic stress on the integrity of hippocampal circuitry in influencing the vulnerability to, or resiliency against, neuronal injury are poorly understood. Here we investigated whether acute vs. chronic psychosocial isolation stress or a combination of the two (chronic stress followed by acute stress) influences the expression of the interneuronal marker parvalbumin (PV) and the chaperone-inducible heat shock protein 70 (Hsp70i) in different subregions of the hippocampus. Low levels of the Ca2+-binding protein (PV) may increase the vulnerability to neuronal injury, and Hsp70i represents an indicator of intense excitation-induced neuronal stress. Adult male Wistar rats were exposed to 2 h of immobilization (IM) or cold (4 degrees C) (acute stressors), 21 d of social isolation (chronic stress), or a combination of both acute and chronic stress. Both chronic isolation and the combined stressors strongly decreased the PV-Immunoreactive cells in the CA1, CA3 and dentate gyrus (DG) region of the hippocampus, while acute stress did not affect PV expression. The combination of acute and chronic stress induced a dramatic increase in Hsp70i expression in the DG, but Hsp70i expression was unaffected in acute and chronic stress alone. We also monitored serum corticosterone (CORT) levels as a neuroendocrine marker of the stress response. Acute stress increased CORT levels, while chronic isolation stress compromised hypothalamic pituitary adrenocortical (HPA) axis activity such that the normal stress response was impaired following subsequent acute stress. These results indicate that in contrast to acute stress, chronic isolation compromises the HPA axis and generates a considerable reduction in PV expression, representing a decrease in the calcium-buffering capacity and a putatively higher vulnerability of specific hippocampal interneurons to excitotoxic injury. The induction of Hsp70i expression in response to acute and chronic isolation reveals that neurons in the DG are particularly vulnerable to an acute stressor following a chronic perturbation of HPA activity. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.",
journal = "Neuroscience",
title = "The Differential Effects of Acute Vs. Chronic Stress and Their Combination on Hippocampal Parvalbumin and Inducible Heat Shock Protein 70 Expression",
volume = "236",
pages = "47-54",
doi = "10.1016/j.neuroscience.2013.01.033"
}

Filipović, D., Martinović, J., Gass, P.,& Inta, D.. (2013). The Differential Effects of Acute Vs. Chronic Stress and Their Combination on Hippocampal Parvalbumin and Inducible Heat Shock Protein 70 Expression. in Neuroscience, 236, 47-54.
https://doi.org/10.1016/j.neuroscience.2013.01.033

Filipović D, Martinović J, Gass P, Inta D. The Differential Effects of Acute Vs. Chronic Stress and Their Combination on Hippocampal Parvalbumin and Inducible Heat Shock Protein 70 Expression. in Neuroscience. 2013;236:47-54.
doi:10.1016/j.neuroscience.2013.01.033 .

Filipović, Dragana, Martinović, Jelena, Gass, Peter, Inta, Dragos, "The Differential Effects of Acute Vs. Chronic Stress and Their Combination on Hippocampal Parvalbumin and Inducible Heat Shock Protein 70 Expression" in Neuroscience, 236 (2013):47-54,
https://doi.org/10.1016/j.neuroscience.2013.01.033 . .

TY  - JOUR
AU  - Jasnic, N.
AU  - Đorđević, Jelena D.
AU  - Djurasevic, S.
AU  - Lakic, I.
AU  - Vujovic, P.
AU  - Spasojević, Nataša
AU  - Cvijic, G.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5003
AB  - The response of hypothalamo-pituitary-adrenocortical (HPA) axis to different stressors depends on numerous stimulatory and inhibitory signals gathering from various parts of the brain to the hypothalamic nuclei. The present study was aimed at determining whether catecholamines (CA) and vasopressin (VP) play the role in the specific regulation of adrenocorticotropic hormone (ACTH) secretion under the influence of thermal stressors, cold (+4 degrees C) and heat (+38 degrees C), applied acutely for 1 h or repeatedly during 7 and 14 day (1 h daily). The results showed that following acute exposure to those stressors, hypothalamic dopamine (DA), noradrenaline (NA) and adrenaline (ADR) concentrations were significantly decreased as compared to non stressed controls. The prolonged exposure to either of the two stressors left hypothalamic CA concentration unaffected. The amount of pituitary VP significantly increased only under the influence of acute heat stress. Prolonged exposure to both stressors induced significant decrease in the pituitary VP content. Unlike the heat, the cold-caused changes in circulating VP did not follow those in the pituitary. The applied stressors significantly increased the amount of the pituitary V1b receptor (V1bR) mainly present at the surface of corticotrophs, depending on both duration of exposure and nature of stressor. Additionally, both cold and heat specifically induced an increase in blood ACTH. In conclusion, this studys results suggest that the role of VP in the regulation of the ACTH secretion in response to cold and heat depends on the type of stressor, whereas the role of the CA depends on the manner of exposure. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Journal of Thermal Biology
T1  - Specific regulation of ACTH secretion under the influence of low and high ambient temperature-The role of catecholamines and vasopressin
VL  - 37
IS  - 7
SP  - 469
EP  - 474
DO  - 10.1016/j.jtherbio.2012.04.003
ER  - 

@article{
author = "Jasnic, N. and Đorđević, Jelena D. and Djurasevic, S. and Lakic, I. and Vujovic, P. and Spasojević, Nataša and Cvijic, G.",
year = "2012",
abstract = "The response of hypothalamo-pituitary-adrenocortical (HPA) axis to different stressors depends on numerous stimulatory and inhibitory signals gathering from various parts of the brain to the hypothalamic nuclei. The present study was aimed at determining whether catecholamines (CA) and vasopressin (VP) play the role in the specific regulation of adrenocorticotropic hormone (ACTH) secretion under the influence of thermal stressors, cold (+4 degrees C) and heat (+38 degrees C), applied acutely for 1 h or repeatedly during 7 and 14 day (1 h daily). The results showed that following acute exposure to those stressors, hypothalamic dopamine (DA), noradrenaline (NA) and adrenaline (ADR) concentrations were significantly decreased as compared to non stressed controls. The prolonged exposure to either of the two stressors left hypothalamic CA concentration unaffected. The amount of pituitary VP significantly increased only under the influence of acute heat stress. Prolonged exposure to both stressors induced significant decrease in the pituitary VP content. Unlike the heat, the cold-caused changes in circulating VP did not follow those in the pituitary. The applied stressors significantly increased the amount of the pituitary V1b receptor (V1bR) mainly present at the surface of corticotrophs, depending on both duration of exposure and nature of stressor. Additionally, both cold and heat specifically induced an increase in blood ACTH. In conclusion, this studys results suggest that the role of VP in the regulation of the ACTH secretion in response to cold and heat depends on the type of stressor, whereas the role of the CA depends on the manner of exposure. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Journal of Thermal Biology",
title = "Specific regulation of ACTH secretion under the influence of low and high ambient temperature-The role of catecholamines and vasopressin",
volume = "37",
number = "7",
pages = "469-474",
doi = "10.1016/j.jtherbio.2012.04.003"
}

Jasnic, N., Đorđević, J. D., Djurasevic, S., Lakic, I., Vujovic, P., Spasojević, N.,& Cvijic, G.. (2012). Specific regulation of ACTH secretion under the influence of low and high ambient temperature-The role of catecholamines and vasopressin. in Journal of Thermal Biology, 37(7), 469-474.
https://doi.org/10.1016/j.jtherbio.2012.04.003

Jasnic N, Đorđević JD, Djurasevic S, Lakic I, Vujovic P, Spasojević N, Cvijic G. Specific regulation of ACTH secretion under the influence of low and high ambient temperature-The role of catecholamines and vasopressin. in Journal of Thermal Biology. 2012;37(7):469-474.
doi:10.1016/j.jtherbio.2012.04.003 .

Jasnic, N., Đorđević, Jelena D., Djurasevic, S., Lakic, I., Vujovic, P., Spasojević, Nataša, Cvijic, G., "Specific regulation of ACTH secretion under the influence of low and high ambient temperature-The role of catecholamines and vasopressin" in Journal of Thermal Biology, 37, no. 7 (2012):469-474,
https://doi.org/10.1016/j.jtherbio.2012.04.003 . .

TY  - JOUR
AU  - Đorđević, Jelena D.
AU  - Jasnic, N.
AU  - Vujovic, P.
AU  - Lakic, I.
AU  - Djurasevic, S.
AU  - Gavrilović, Ljubica
AU  - Cvijic, G.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4647
AB  - The heart is an organ especially sensitive to the sympathetic overstimulation and therefore to the influence of stressors and hypertension. The aim of the present study was to investigate the effect of two distinct types of stressors, acute immobilization (2 h) and chronic isolation stress (21 days), as well as their combined effect on the activity of monoamine oxidase (MAO), superoxide dismutase, catalase (CAT) and the ascorbic acid (AA) content in the heart of normotensive and spontaneously hypertensive rats (SHR). The results obtained show that in basal conditions heart MAO and CAT activity (p LT 0.05), as well as AA concentration (p LT 0.01) were higher in SHR than in normotensive ones. The acute immobilization significantly decreased heart MAO activity in both examined strains (p LT 0.01). On the other hand, chronic isolation, separately or in combination with immobilization, did not affect this enzyme, in the heart of either hypertensive or normotensive rats, which was associated with the reduced antioxidative protection (p LT 0.01, p LT 0.05).
T2  - Journal of Animal Physiology and Animal Nutrition
T1  - Distinct and combined effects of acute immobilization and chronic isolation stress on MAO activity and antioxidative protection in the heart of normotensive and spontaneously hypertensive rats
VL  - 96
IS  - 1
SP  - 58
EP  - 65
DO  - 10.1111/j.1439-0396.2010.01122.x
ER  - 

@article{
author = "Đorđević, Jelena D. and Jasnic, N. and Vujovic, P. and Lakic, I. and Djurasevic, S. and Gavrilović, Ljubica and Cvijic, G.",
year = "2012",
abstract = "The heart is an organ especially sensitive to the sympathetic overstimulation and therefore to the influence of stressors and hypertension. The aim of the present study was to investigate the effect of two distinct types of stressors, acute immobilization (2 h) and chronic isolation stress (21 days), as well as their combined effect on the activity of monoamine oxidase (MAO), superoxide dismutase, catalase (CAT) and the ascorbic acid (AA) content in the heart of normotensive and spontaneously hypertensive rats (SHR). The results obtained show that in basal conditions heart MAO and CAT activity (p LT 0.05), as well as AA concentration (p LT 0.01) were higher in SHR than in normotensive ones. The acute immobilization significantly decreased heart MAO activity in both examined strains (p LT 0.01). On the other hand, chronic isolation, separately or in combination with immobilization, did not affect this enzyme, in the heart of either hypertensive or normotensive rats, which was associated with the reduced antioxidative protection (p LT 0.01, p LT 0.05).",
journal = "Journal of Animal Physiology and Animal Nutrition",
title = "Distinct and combined effects of acute immobilization and chronic isolation stress on MAO activity and antioxidative protection in the heart of normotensive and spontaneously hypertensive rats",
volume = "96",
number = "1",
pages = "58-65",
doi = "10.1111/j.1439-0396.2010.01122.x"
}

Đorđević, J. D., Jasnic, N., Vujovic, P., Lakic, I., Djurasevic, S., Gavrilović, L.,& Cvijic, G.. (2012). Distinct and combined effects of acute immobilization and chronic isolation stress on MAO activity and antioxidative protection in the heart of normotensive and spontaneously hypertensive rats. in Journal of Animal Physiology and Animal Nutrition, 96(1), 58-65.
https://doi.org/10.1111/j.1439-0396.2010.01122.x

Đorđević JD, Jasnic N, Vujovic P, Lakic I, Djurasevic S, Gavrilović L, Cvijic G. Distinct and combined effects of acute immobilization and chronic isolation stress on MAO activity and antioxidative protection in the heart of normotensive and spontaneously hypertensive rats. in Journal of Animal Physiology and Animal Nutrition. 2012;96(1):58-65.
doi:10.1111/j.1439-0396.2010.01122.x .

Đorđević, Jelena D., Jasnic, N., Vujovic, P., Lakic, I., Djurasevic, S., Gavrilović, Ljubica, Cvijic, G., "Distinct and combined effects of acute immobilization and chronic isolation stress on MAO activity and antioxidative protection in the heart of normotensive and spontaneously hypertensive rats" in Journal of Animal Physiology and Animal Nutrition, 96, no. 1 (2012):58-65,
https://doi.org/10.1111/j.1439-0396.2010.01122.x . .

TY  - JOUR
AU  - Martinović, Jelena
AU  - Filipović, Dragana
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5088
AB  - Mitochondrial dysfunction has been implicated in several psychiatric disorders, including depression. Given that the B-cell-lymphoma 2 (Bcl-2) protein family plays a role in the regulation of mitochondrial apoptotic pathway, we hypothesized that ratio of proapoptotic to antiapoptotic proteins (e.g., Bcl-2-associated X protein (Bax)/Bcl-2) may determine prosurvival/proapoptotic intracellular signaling under stress. We tested this hypothesis by examining the effects of 2 h of acute stress immobilization (IM) or cold (C), 21 days of social isolation as chronic stress and combined stress (chronic stress followed by acute stress) on cytosolic/mitochondrial levels and ratios of Bax and Bcl-2 proteins in relation to cytosolic nitric oxide (NO) metabolites (nitrates and nitrites) and p53 protein redistribution between cytosolic and mitochondrial compartments in the prefrontal cortex (PFC) and hippocampus (HIPP) of male Wistar rats. The stress-induced changes in serum corticosterone (CORT) concentrations were also followed. Acute stressors resulting in an elevated CORT level did not change the Bax/Bcl-2 ratio in either brain region. However, chronic isolation, resulting in CORT levels similar to basal values, led to a translocation of mitochondrial Bcl-2 to the cytosol in the PFC. Furthermore, the Bax/Bcl-2 ratio in the PFC was significantly increased following chronic isolation and remained elevated after combined stressors. NO metabolites were increased by chronic isolation and the two combined stressors in the HIPP and following the combined stressors in the PFC. Translocation of p53 and proapoptotic Bax from the cytosol into mitochondria in response to NO overproduction following combined stressors was detected only in the PFC. These data indicate that chronic isolation stress exerts opposing actions on p53 and NO mechanisms in a tissue-specific manner (PFC vs. HIPP), triggering proapoptotic signaling via Bcl-2 translocation in the PFC. (c) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.
T2  - Neuroscience
T1  - Bax and B-Cell-Lymphoma 2 Mediate Proapoptotic Signaling Following Chronic Isolation Stress in Rat Brain
VL  - 223
SP  - 238
EP  - 245
DO  - 10.1016/j.neuroscience.2012.08.005
ER  - 

@article{
author = "Martinović, Jelena and Filipović, Dragana",
year = "2012",
abstract = "Mitochondrial dysfunction has been implicated in several psychiatric disorders, including depression. Given that the B-cell-lymphoma 2 (Bcl-2) protein family plays a role in the regulation of mitochondrial apoptotic pathway, we hypothesized that ratio of proapoptotic to antiapoptotic proteins (e.g., Bcl-2-associated X protein (Bax)/Bcl-2) may determine prosurvival/proapoptotic intracellular signaling under stress. We tested this hypothesis by examining the effects of 2 h of acute stress immobilization (IM) or cold (C), 21 days of social isolation as chronic stress and combined stress (chronic stress followed by acute stress) on cytosolic/mitochondrial levels and ratios of Bax and Bcl-2 proteins in relation to cytosolic nitric oxide (NO) metabolites (nitrates and nitrites) and p53 protein redistribution between cytosolic and mitochondrial compartments in the prefrontal cortex (PFC) and hippocampus (HIPP) of male Wistar rats. The stress-induced changes in serum corticosterone (CORT) concentrations were also followed. Acute stressors resulting in an elevated CORT level did not change the Bax/Bcl-2 ratio in either brain region. However, chronic isolation, resulting in CORT levels similar to basal values, led to a translocation of mitochondrial Bcl-2 to the cytosol in the PFC. Furthermore, the Bax/Bcl-2 ratio in the PFC was significantly increased following chronic isolation and remained elevated after combined stressors. NO metabolites were increased by chronic isolation and the two combined stressors in the HIPP and following the combined stressors in the PFC. Translocation of p53 and proapoptotic Bax from the cytosol into mitochondria in response to NO overproduction following combined stressors was detected only in the PFC. These data indicate that chronic isolation stress exerts opposing actions on p53 and NO mechanisms in a tissue-specific manner (PFC vs. HIPP), triggering proapoptotic signaling via Bcl-2 translocation in the PFC. (c) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.",
journal = "Neuroscience",
title = "Bax and B-Cell-Lymphoma 2 Mediate Proapoptotic Signaling Following Chronic Isolation Stress in Rat Brain",
volume = "223",
pages = "238-245",
doi = "10.1016/j.neuroscience.2012.08.005"
}

Martinović, J.,& Filipović, D.. (2012). Bax and B-Cell-Lymphoma 2 Mediate Proapoptotic Signaling Following Chronic Isolation Stress in Rat Brain. in Neuroscience, 223, 238-245.
https://doi.org/10.1016/j.neuroscience.2012.08.005

Martinović J, Filipović D. Bax and B-Cell-Lymphoma 2 Mediate Proapoptotic Signaling Following Chronic Isolation Stress in Rat Brain. in Neuroscience. 2012;223:238-245.
doi:10.1016/j.neuroscience.2012.08.005 .

Martinović, Jelena, Filipović, Dragana, "Bax and B-Cell-Lymphoma 2 Mediate Proapoptotic Signaling Following Chronic Isolation Stress in Rat Brain" in Neuroscience, 223 (2012):238-245,
https://doi.org/10.1016/j.neuroscience.2012.08.005 . .

TY  - CONF
AU  - Zlatković, Jelena
AU  - Demajo, Miroslav
AU  - Filipović, Dragana
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9282
AB  - Nitric oxide (NO) has been identified as a source of oxidative/nitrosative stress that
is known to oxidatively modify DNA, lipids and proteins. One such modification is
the addition of carbonyl groups to amino acid residues in proteins. Therefore, the
aim of our study was to examine the effects of acute, chronic or combined stress on
NO production and protein carbonyl content in the cytosolic fraction of rat liver.
Since NO is a highly reactive molecule, the levels of NO metabolites (nitrates and
nitrites) as markers of stable end products of NO metabolism were measured. Both
acute stresses showed unchanged nitrite levels while only acute IM stress led to an
increased level of the carbonyl group. The NO metabolites and protein carbonyl
content were increased by chronic isolation and remained upregulated after
combined stress. These data indicate that chronic isolation stress with increased
NO metabolites led to nitrosative stress, whereby accumulation of oxidized
proteins in the liver may induce progressive liver damage.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
T1  - Nitric oxide and protein carbonyl content in the liver of stressed rats
VL  - 1
SP  - 358
EP  - 360
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9282
ER  - 

@conference{
author = "Zlatković, Jelena and Demajo, Miroslav and Filipović, Dragana",
year = "2012",
abstract = "Nitric oxide (NO) has been identified as a source of oxidative/nitrosative stress that
is known to oxidatively modify DNA, lipids and proteins. One such modification is
the addition of carbonyl groups to amino acid residues in proteins. Therefore, the
aim of our study was to examine the effects of acute, chronic or combined stress on
NO production and protein carbonyl content in the cytosolic fraction of rat liver.
Since NO is a highly reactive molecule, the levels of NO metabolites (nitrates and
nitrites) as markers of stable end products of NO metabolism were measured. Both
acute stresses showed unchanged nitrite levels while only acute IM stress led to an
increased level of the carbonyl group. The NO metabolites and protein carbonyl
content were increased by chronic isolation and remained upregulated after
combined stress. These data indicate that chronic isolation stress with increased
NO metabolites led to nitrosative stress, whereby accumulation of oxidized
proteins in the liver may induce progressive liver damage.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry",
title = "Nitric oxide and protein carbonyl content in the liver of stressed rats",
volume = "1",
pages = "358-360",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9282"
}

Zlatković, J., Demajo, M.,& Filipović, D.. (2012). Nitric oxide and protein carbonyl content in the liver of stressed rats. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 358-360.
https://hdl.handle.net/21.15107/rcub_vinar_9282

Zlatković J, Demajo M, Filipović D. Nitric oxide and protein carbonyl content in the liver of stressed rats. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry. 2012;1:358-360.
https://hdl.handle.net/21.15107/rcub_vinar_9282 .

Zlatković, Jelena, Demajo, Miroslav, Filipović, Dragana, "Nitric oxide and protein carbonyl content in the liver of stressed rats" in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry, 1 (2012):358-360,
https://hdl.handle.net/21.15107/rcub_vinar_9282 .

TY  - JOUR
AU  - Lakić, Iva
AU  - Drenca, Tamara
AU  - Đorđević, Jelena D.
AU  - Vujovic, P.
AU  - Jasnic, N.
AU  - Djurasevic, S.
AU  - Dronjak, Slađana
AU  - Cvijic, Gordana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4466
AB  - Interscapular brown adipose tissue (IBAT) is an energy storing organ involved in the maintenance of homeostasis in stress conditions when the balance of energy supplies is disturbed. The major regulator of IBAT activity is the sympathetic nervous system (SNS). Since genetic background is responsible for the individual differences in neuroendocrine stress responsivity, spontaneously hypertensive rats (SHR) that have a genetically increased general sympathetic output are a useful model for studying adaptive processes in stress conditions. Our aim was to test the effect of acute and/or chronic exposure to various stressors (thermal-cold, psychophysical-immobilization and psychosocial-isolation) on IBAT SNS and the metabolic activity in SHR, by measuring the number of monoamine-containing nerve endings and uncoupling protein-1 (UCP-1) content. The obtained results show that the IBAT SNS activity of unstressed SHR was stimulated by the administration of a single acute or chronic stressor and was independent of the duration or type of stressor, while chronic pre-stress of isolation suppressed further the SNS reaction to novel acute stress exposure. The IBAT UCP-1 content followed SNS changes, suggesting that this system is dominant in the regulation of IBAT metabolic rate in SHR.
T2  - Archives of Biological Sciences
T1  - Novel Acute Stressor Effects on Interscapular Brown Adipose Tissue Sympathetic Inervation and Ucp-1 Content in Chronically Isolated and Spontaneously Hypertensive Rats
VL  - 63
IS  - 3
SP  - 589
EP  - 596
DO  - 10.2298/ABS1103589L
ER  - 

@article{
author = "Lakić, Iva and Drenca, Tamara and Đorđević, Jelena D. and Vujovic, P. and Jasnic, N. and Djurasevic, S. and Dronjak, Slađana and Cvijic, Gordana",
year = "2011",
abstract = "Interscapular brown adipose tissue (IBAT) is an energy storing organ involved in the maintenance of homeostasis in stress conditions when the balance of energy supplies is disturbed. The major regulator of IBAT activity is the sympathetic nervous system (SNS). Since genetic background is responsible for the individual differences in neuroendocrine stress responsivity, spontaneously hypertensive rats (SHR) that have a genetically increased general sympathetic output are a useful model for studying adaptive processes in stress conditions. Our aim was to test the effect of acute and/or chronic exposure to various stressors (thermal-cold, psychophysical-immobilization and psychosocial-isolation) on IBAT SNS and the metabolic activity in SHR, by measuring the number of monoamine-containing nerve endings and uncoupling protein-1 (UCP-1) content. The obtained results show that the IBAT SNS activity of unstressed SHR was stimulated by the administration of a single acute or chronic stressor and was independent of the duration or type of stressor, while chronic pre-stress of isolation suppressed further the SNS reaction to novel acute stress exposure. The IBAT UCP-1 content followed SNS changes, suggesting that this system is dominant in the regulation of IBAT metabolic rate in SHR.",
journal = "Archives of Biological Sciences",
title = "Novel Acute Stressor Effects on Interscapular Brown Adipose Tissue Sympathetic Inervation and Ucp-1 Content in Chronically Isolated and Spontaneously Hypertensive Rats",
volume = "63",
number = "3",
pages = "589-596",
doi = "10.2298/ABS1103589L"
}

Lakić, I., Drenca, T., Đorđević, J. D., Vujovic, P., Jasnic, N., Djurasevic, S., Dronjak, S.,& Cvijic, G.. (2011). Novel Acute Stressor Effects on Interscapular Brown Adipose Tissue Sympathetic Inervation and Ucp-1 Content in Chronically Isolated and Spontaneously Hypertensive Rats. in Archives of Biological Sciences, 63(3), 589-596.
https://doi.org/10.2298/ABS1103589L

Lakić I, Drenca T, Đorđević JD, Vujovic P, Jasnic N, Djurasevic S, Dronjak S, Cvijic G. Novel Acute Stressor Effects on Interscapular Brown Adipose Tissue Sympathetic Inervation and Ucp-1 Content in Chronically Isolated and Spontaneously Hypertensive Rats. in Archives of Biological Sciences. 2011;63(3):589-596.
doi:10.2298/ABS1103589L .

Lakić, Iva, Drenca, Tamara, Đorđević, Jelena D., Vujovic, P., Jasnic, N., Djurasevic, S., Dronjak, Slađana, Cvijic, Gordana, "Novel Acute Stressor Effects on Interscapular Brown Adipose Tissue Sympathetic Inervation and Ucp-1 Content in Chronically Isolated and Spontaneously Hypertensive Rats" in Archives of Biological Sciences, 63, no. 3 (2011):589-596,
https://doi.org/10.2298/ABS1103589L . .

TY  - JOUR
AU  - Spasojević, Nataša
AU  - Jovanović, Predrag
AU  - Spasojević-Tišma, Vera D.
AU  - Delic, N.
AU  - Dronjak, Slađana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4467
AB  - It is well known that antidepressants affect central monoaminergic neurotransmission and that they also modulate hormone release in peripheral tissues. Repeated maprotiline (a noradrenaline reuptake inhibitor) and fluoxetine (a serotonin reuptake inhibitor) treatment on gene expression of the catecholamine biosynthetic enzymes were examined in rat atria and ventricles in vivo. Maprotiline decreased the gene expression of tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase (DBH) in the rat atrium. Fluoxetine increased gene expression of TH and DBH, but not of phenylethanolamine N-methyltransferase (PNMT). Chronic application of antidepressants did not change the expression of these enzymes in the ventricles. We conclude that repeated administration of fluoxetine enhances gene transcription of TH and DBH and subsequently stimulates noradrenaline synthesis in rat atria in vivo.
T2  - Archives of Biological Sciences
T1  - Differential in Vivo Regulation of Th and Dbh Mrna in Rat Atria By Maprotiline and Fluoxetine
VL  - 63
IS  - 3
SP  - 597
EP  - 601
DO  - 10.2298/ABS1103597S
ER  - 

@article{
author = "Spasojević, Nataša and Jovanović, Predrag and Spasojević-Tišma, Vera D. and Delic, N. and Dronjak, Slađana",
year = "2011",
abstract = "It is well known that antidepressants affect central monoaminergic neurotransmission and that they also modulate hormone release in peripheral tissues. Repeated maprotiline (a noradrenaline reuptake inhibitor) and fluoxetine (a serotonin reuptake inhibitor) treatment on gene expression of the catecholamine biosynthetic enzymes were examined in rat atria and ventricles in vivo. Maprotiline decreased the gene expression of tyrosine hydroxylase (TH) and dopamine-beta-hydroxylase (DBH) in the rat atrium. Fluoxetine increased gene expression of TH and DBH, but not of phenylethanolamine N-methyltransferase (PNMT). Chronic application of antidepressants did not change the expression of these enzymes in the ventricles. We conclude that repeated administration of fluoxetine enhances gene transcription of TH and DBH and subsequently stimulates noradrenaline synthesis in rat atria in vivo.",
journal = "Archives of Biological Sciences",
title = "Differential in Vivo Regulation of Th and Dbh Mrna in Rat Atria By Maprotiline and Fluoxetine",
volume = "63",
number = "3",
pages = "597-601",
doi = "10.2298/ABS1103597S"
}

Spasojević, N., Jovanović, P., Spasojević-Tišma, V. D., Delic, N.,& Dronjak, S.. (2011). Differential in Vivo Regulation of Th and Dbh Mrna in Rat Atria By Maprotiline and Fluoxetine. in Archives of Biological Sciences, 63(3), 597-601.
https://doi.org/10.2298/ABS1103597S

Spasojević N, Jovanović P, Spasojević-Tišma VD, Delic N, Dronjak S. Differential in Vivo Regulation of Th and Dbh Mrna in Rat Atria By Maprotiline and Fluoxetine. in Archives of Biological Sciences. 2011;63(3):597-601.
doi:10.2298/ABS1103597S .

Spasojević, Nataša, Jovanović, Predrag, Spasojević-Tišma, Vera D., Delic, N., Dronjak, Slađana, "Differential in Vivo Regulation of Th and Dbh Mrna in Rat Atria By Maprotiline and Fluoxetine" in Archives of Biological Sciences, 63, no. 3 (2011):597-601,
https://doi.org/10.2298/ABS1103597S . .

TY  - JOUR
AU  - Martinović, Jelena
AU  - Filipović, Dragana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4347
AB  - Exposure to different stressors initiates generation of reactive oxygen species (ROS), which create harmful environment for cellular macromolecules. Superoxide dismutases (SODs) represent the first line of antioxidant defense. Hence, any alternation in their function might be potentially damaging. To better define the role of SODs, we investigated the CuZnSOD activity in cytosolic and the nuclear fraction as well as mitochondrial MnSOD activity in the liver of Wistar male rats after exposure to 2 h of acute immobilization (IM) or cold (4A degrees C) stress, 21 days of chronic social isolation (IS) or their combination (chronic stress followed by acute stress). Serum corticosterone (CORT) was monitored as an indicator of the stress response. Acute IM stress, with elevated CORT level, led to increased hepatic CuZnSOD activity in the nuclear fraction. Chronic isolation stress, where CORT was close to control value, did not change the CuZnSOD activity either in nuclei or in cytosolic fraction, while combined stress IS+Cold led to increased cytosolic CuZnSOD activity. MnSOD activity in mitochondrial fraction was decreased in all treated groups. Data have shown that different stressors have diverse effect on hepatic CuZnSOD and MnSOD activity as well as on serum CORT level. Increased nuclear CuZnSOD activity after acute stress represents physiological response since the named activity protects cells against oxidative stress, while chronic IS stress compromises CuZnSOD function, suggesting an inefficient defense against ROS. Observed decrease of MnSOD activities indicate inadequate elimination of ROS after acute or chronic stress, which is characteristic of the oxidative stress.
T2  - Molecular and Cellular Biochemistry
T1  - Stress-induced alternations in CuZnSOD and MnSOD activity in cellular compartments of rat liver
VL  - 357
IS  - 1-2
SP  - 143
EP  - 150
DO  - 10.1007/s11010-011-0884-4
ER  - 

@article{
author = "Martinović, Jelena and Filipović, Dragana",
year = "2011",
abstract = "Exposure to different stressors initiates generation of reactive oxygen species (ROS), which create harmful environment for cellular macromolecules. Superoxide dismutases (SODs) represent the first line of antioxidant defense. Hence, any alternation in their function might be potentially damaging. To better define the role of SODs, we investigated the CuZnSOD activity in cytosolic and the nuclear fraction as well as mitochondrial MnSOD activity in the liver of Wistar male rats after exposure to 2 h of acute immobilization (IM) or cold (4A degrees C) stress, 21 days of chronic social isolation (IS) or their combination (chronic stress followed by acute stress). Serum corticosterone (CORT) was monitored as an indicator of the stress response. Acute IM stress, with elevated CORT level, led to increased hepatic CuZnSOD activity in the nuclear fraction. Chronic isolation stress, where CORT was close to control value, did not change the CuZnSOD activity either in nuclei or in cytosolic fraction, while combined stress IS+Cold led to increased cytosolic CuZnSOD activity. MnSOD activity in mitochondrial fraction was decreased in all treated groups. Data have shown that different stressors have diverse effect on hepatic CuZnSOD and MnSOD activity as well as on serum CORT level. Increased nuclear CuZnSOD activity after acute stress represents physiological response since the named activity protects cells against oxidative stress, while chronic IS stress compromises CuZnSOD function, suggesting an inefficient defense against ROS. Observed decrease of MnSOD activities indicate inadequate elimination of ROS after acute or chronic stress, which is characteristic of the oxidative stress.",
journal = "Molecular and Cellular Biochemistry",
title = "Stress-induced alternations in CuZnSOD and MnSOD activity in cellular compartments of rat liver",
volume = "357",
number = "1-2",
pages = "143-150",
doi = "10.1007/s11010-011-0884-4"
}

Martinović, J.,& Filipović, D.. (2011). Stress-induced alternations in CuZnSOD and MnSOD activity in cellular compartments of rat liver. in Molecular and Cellular Biochemistry, 357(1-2), 143-150.
https://doi.org/10.1007/s11010-011-0884-4

Martinović J, Filipović D. Stress-induced alternations in CuZnSOD and MnSOD activity in cellular compartments of rat liver. in Molecular and Cellular Biochemistry. 2011;357(1-2):143-150.
doi:10.1007/s11010-011-0884-4 .

Martinović, Jelena, Filipović, Dragana, "Stress-induced alternations in CuZnSOD and MnSOD activity in cellular compartments of rat liver" in Molecular and Cellular Biochemistry, 357, no. 1-2 (2011):143-150,
https://doi.org/10.1007/s11010-011-0884-4 . .