TY  - CONF
AU  - Horvat, Anica
AU  - Milošević, Maja
AU  - Petrović, Snježana
AU  - Demajo, Miroslav
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9499
AB  - Ions of zinc and cooper represents micro elements present in all organisms but they can be accumulated in various tissues from the environment by food or pollution. In this work we examined the effects of chloride salts of Cu2+ and Zn2+ on the activity of ecto-ATPase, integral plasma membrane protein, in brain and ovarian cells. Both ions exhibit similar effects on brain or ovarian enzyme activity. Cooper in brain and ovary totally inhibits enzyme activity at the concentration of 1 mM and 0.1 M respectively. IC50 for brain is 36 µM while in ovary it is 192 µM. Zinc inhibits in both tissues 50% of the control enzyme activity at concentrations of 1 mM in brain and 0.1 M in ovary with IC50 of 1mM and 14 mM respectively. These metals possess an affinity for -SH groups in the enzyme protein, may replace Mg2+ in enzyme substrate, MgATP or to bind for enzyme substrate site. According to the total inhibition of ecto-ATPase activity by Cu 2+, it may be proposed that its site of action is on the substrate or substrate binding site, while Zn2+ modulates enzyme activity by acting on the –SH or S-S groups of the enzyme. Inhibiting or decreasing ecto-ATPase activity in brain and ovary, these two metal ions may be toxic and seriously disturb the proper functioning of the investigated tissues.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+
VL  - 1
SP  - 374
EP  - 376
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9499
ER  - 

@conference{
author = "Horvat, Anica and Milošević, Maja and Petrović, Snježana and Demajo, Miroslav",
year = "2004",
abstract = "Ions of zinc and cooper represents micro elements present in all organisms but they can be accumulated in various tissues from the environment by food or pollution. In this work we examined the effects of chloride salts of Cu2+ and Zn2+ on the activity of ecto-ATPase, integral plasma membrane protein, in brain and ovarian cells. Both ions exhibit similar effects on brain or ovarian enzyme activity. Cooper in brain and ovary totally inhibits enzyme activity at the concentration of 1 mM and 0.1 M respectively. IC50 for brain is 36 µM while in ovary it is 192 µM. Zinc inhibits in both tissues 50% of the control enzyme activity at concentrations of 1 mM in brain and 0.1 M in ovary with IC50 of 1mM and 14 mM respectively. These metals possess an affinity for -SH groups in the enzyme protein, may replace Mg2+ in enzyme substrate, MgATP or to bind for enzyme substrate site. According to the total inhibition of ecto-ATPase activity by Cu 2+, it may be proposed that its site of action is on the substrate or substrate binding site, while Zn2+ modulates enzyme activity by acting on the –SH or S-S groups of the enzyme. Inhibiting or decreasing ecto-ATPase activity in brain and ovary, these two metal ions may be toxic and seriously disturb the proper functioning of the investigated tissues.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+",
volume = "1",
pages = "374-376",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9499"
}

Horvat, A., Milošević, M., Petrović, S.,& Demajo, M.. (2004). Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 374-376.
https://hdl.handle.net/21.15107/rcub_vinar_9499

Horvat A, Milošević M, Petrović S, Demajo M. Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:374-376.
https://hdl.handle.net/21.15107/rcub_vinar_9499 .

Horvat, Anica, Milošević, Maja, Petrović, Snježana, Demajo, Miroslav, "Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):374-376,
https://hdl.handle.net/21.15107/rcub_vinar_9499 .

TY  - CONF
AU  - Demajo, Miroslav
AU  - Đorđević, D. S.
AU  - Đujić, Ivana S.
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9531
AB  - ct Irradiating the head region of 8-day-old rats with a dose of 8.64Gy of X-rays, at the age of 42 days, results in stunting of lower jaw growth as illustrated by measuring the length and height of the jaws. This irradiation dose also has as a consequence an increased content of Ca 2+ in the bone tissue of the lower jaw (270.36±27.97 mmol/kg bone tissue) when compared to the corresponding non-irradiated controls (188.58±19.26 mmol/kg bone tissue, p<0.05) and rats irradiated with a dose of 3.84Gy of X-rays (143.99±15.29 mmol/kg bone tissue, p<0.01).
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - Calcium (Ca2+) content in the lower jaws of rats irradiated in the head region with X-rays
VL  - 1
SP  - 453
EP  - 455
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9531
ER  - 

@conference{
author = "Demajo, Miroslav and Đorđević, D. S. and Đujić, Ivana S.",
year = "2004",
abstract = "ct Irradiating the head region of 8-day-old rats with a dose of 8.64Gy of X-rays, at the age of 42 days, results in stunting of lower jaw growth as illustrated by measuring the length and height of the jaws. This irradiation dose also has as a consequence an increased content of Ca 2+ in the bone tissue of the lower jaw (270.36±27.97 mmol/kg bone tissue) when compared to the corresponding non-irradiated controls (188.58±19.26 mmol/kg bone tissue, p<0.05) and rats irradiated with a dose of 3.84Gy of X-rays (143.99±15.29 mmol/kg bone tissue, p<0.01).",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Calcium (Ca2+) content in the lower jaws of rats irradiated in the head region with X-rays",
volume = "1",
pages = "453-455",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9531"
}

Demajo, M., Đorđević, D. S.,& Đujić, I. S.. (2004). Calcium (Ca2+) content in the lower jaws of rats irradiated in the head region with X-rays. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 453-455.
https://hdl.handle.net/21.15107/rcub_vinar_9531

Demajo M, Đorđević DS, Đujić IS. Calcium (Ca2+) content in the lower jaws of rats irradiated in the head region with X-rays. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:453-455.
https://hdl.handle.net/21.15107/rcub_vinar_9531 .

Demajo, Miroslav, Đorđević, D. S., Đujić, Ivana S., "Calcium (Ca2+) content in the lower jaws of rats irradiated in the head region with X-rays" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):453-455,
https://hdl.handle.net/21.15107/rcub_vinar_9531 .