TY  - JOUR
AU  - Cvetićanin, Jelena M.
AU  - Joksić, Gordana
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Valenta-Šobot, Ana
AU  - Nešković, Olivera M.
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3834
AB  - Carbon nanotubes are unique one-dimensional macromolecules with promising applications in biology and medicine. Since their toxicity is still under debate, here we present a study investigating the genotoxic properties of purified single wall carbon nanotubes (SWCNTs), multiwall carbon nanotubes (MWCNTs), and amide functionalized purified SWCNTs on cultured human lymphocytes employing cytokinesis block micronucleus assay and enumeration of gamma H2AX foci as a measure of double strand breaks (DSBs) of the DNA in normal human fibroblasts. SWCNTs induce micronuclei (MN) formation in lymphocytes and decrease the proliferation potential (CBPI) of cells. In a fibroblast cell line the same dose of SWCNTs induces gamma H2AX foci 2.7-fold higher than in a control. Amide functionalized purified SWCNTs behave differently: they do not disturb the cell proliferation potential of harvested lymphocytes, but induce micronuclei to a higher extent than SWCNTs. When applied on fibroblasts, amide functionalized SWCNTs also induce gamma H2AX foci, 3.18-fold higher than the control. The cellular effects of MWCNTs display the broad spectrum of clastogenic properties seen as the highest incidence of induced lymphocyte micronuclei and anaphase bridges among nuclei in binucleated cells. Surprisingly, the incidence of induced gamma H2AX foci was not as high as was expected by the micronucleus test, which indicates that MWCNTs act as clastogen and aneugen agents simultaneously. Biological endpoints investigated in this study indicate a close relationship between the electrochemical properties of carbon nanotubes and observed genotoxicity.
T2  - Nanotechnology
T1  - Using carbon nanotubes to induce micronuclei and double strand breaks of the DNA in human cells
VL  - 21
IS  - 1
DO  - 10.1088/0957-4484/21/1/015102
ER  - 

@article{
author = "Cvetićanin, Jelena M. and Joksić, Gordana and Leskovac, Andreja and Petrović, Sandra and Valenta-Šobot, Ana and Nešković, Olivera M.",
year = "2010",
abstract = "Carbon nanotubes are unique one-dimensional macromolecules with promising applications in biology and medicine. Since their toxicity is still under debate, here we present a study investigating the genotoxic properties of purified single wall carbon nanotubes (SWCNTs), multiwall carbon nanotubes (MWCNTs), and amide functionalized purified SWCNTs on cultured human lymphocytes employing cytokinesis block micronucleus assay and enumeration of gamma H2AX foci as a measure of double strand breaks (DSBs) of the DNA in normal human fibroblasts. SWCNTs induce micronuclei (MN) formation in lymphocytes and decrease the proliferation potential (CBPI) of cells. In a fibroblast cell line the same dose of SWCNTs induces gamma H2AX foci 2.7-fold higher than in a control. Amide functionalized purified SWCNTs behave differently: they do not disturb the cell proliferation potential of harvested lymphocytes, but induce micronuclei to a higher extent than SWCNTs. When applied on fibroblasts, amide functionalized SWCNTs also induce gamma H2AX foci, 3.18-fold higher than the control. The cellular effects of MWCNTs display the broad spectrum of clastogenic properties seen as the highest incidence of induced lymphocyte micronuclei and anaphase bridges among nuclei in binucleated cells. Surprisingly, the incidence of induced gamma H2AX foci was not as high as was expected by the micronucleus test, which indicates that MWCNTs act as clastogen and aneugen agents simultaneously. Biological endpoints investigated in this study indicate a close relationship between the electrochemical properties of carbon nanotubes and observed genotoxicity.",
journal = "Nanotechnology",
title = "Using carbon nanotubes to induce micronuclei and double strand breaks of the DNA in human cells",
volume = "21",
number = "1",
doi = "10.1088/0957-4484/21/1/015102"
}

Cvetićanin, J. M., Joksić, G., Leskovac, A., Petrović, S., Valenta-Šobot, A.,& Nešković, O. M.. (2010). Using carbon nanotubes to induce micronuclei and double strand breaks of the DNA in human cells. in Nanotechnology, 21(1).
https://doi.org/10.1088/0957-4484/21/1/015102

Cvetićanin JM, Joksić G, Leskovac A, Petrović S, Valenta-Šobot A, Nešković OM. Using carbon nanotubes to induce micronuclei and double strand breaks of the DNA in human cells. in Nanotechnology. 2010;21(1).
doi:10.1088/0957-4484/21/1/015102 .

Cvetićanin, Jelena M., Joksić, Gordana, Leskovac, Andreja, Petrović, Sandra, Valenta-Šobot, Ana, Nešković, Olivera M., "Using carbon nanotubes to induce micronuclei and double strand breaks of the DNA in human cells" in Nanotechnology, 21, no. 1 (2010),
https://doi.org/10.1088/0957-4484/21/1/015102 . .