TY  - JOUR
AU  - Kesavan, Rushendhiran
AU  - Potunuru, Uma Rani
AU  - Nastasijević, Branislav J.
AU  - Avaneesh, T.
AU  - Joksić, Gordana
AU  - Dixit, Madhulika
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5430
AB  - Gentiana lutea belonging to the Gentianaceae family of flowering plants are routinely used in traditional Serbian medicine for their beneficial gastro-intestinal and anti-inflammatory properties. The aim of the study was to determine whether aqueous root extracts of Gentiana lutea consisting of gentiopicroside, gentisin, bellidifolin-8-O-glucoside, demethylbellidifolin-8-O-glucoside, isovitexin, swertiamarin and amarogentin prevents proliferation of aortic smooth muscle cells in response to PDGF-BB. Cell proliferation and cell cycle analysis were performed based on alamar blue assay and propidium iodide labeling respectively. In primary cultures of rat aortic smooth muscle cells (RASMCs), PDGF-BB (20 ng/ml) induced a two-fold increase in cell proliferation which was significantly blocked by the root extract (1 mg/ml). The root extract also prevented the S-phase entry of synchronized cells in response to PDGF. Furthermore, PDGF-BB induced ERK1/2 activation and consequent increase in cellular nitric oxide (NO) levels were also blocked by the extract. These effects of extract were due to blockade of PDGF-BB induced expression of iNOS, cyclin D1 and proliferating cell nuclear antigen (PCNA). Docking analysis of the extract components on MEK1, the upstream ERK1/2 activating kinase using AutoDock4, indicated a likely binding of isovitexin to the inhibitor binding site of MEK1. Experiments performed with purified isovitexin demonstrated that it successfully blocks PDGF-induced ERK1/2 activation and proliferation of RASMCs in cell culture. Thus, Gentiana lutea can provide novel candidates for prevention and treatment of atherosclerosis.
T2  - PLOS One
T1  - Inhibition of Vascular Smooth Muscle Cell Proliferation by Gentiana lutea Root Extracts
VL  - 8
IS  - 4
DO  - 10.1371/journal.pone.0061393
ER  - 

@article{
author = "Kesavan, Rushendhiran and Potunuru, Uma Rani and Nastasijević, Branislav J. and Avaneesh, T. and Joksić, Gordana and Dixit, Madhulika",
year = "2013",
abstract = "Gentiana lutea belonging to the Gentianaceae family of flowering plants are routinely used in traditional Serbian medicine for their beneficial gastro-intestinal and anti-inflammatory properties. The aim of the study was to determine whether aqueous root extracts of Gentiana lutea consisting of gentiopicroside, gentisin, bellidifolin-8-O-glucoside, demethylbellidifolin-8-O-glucoside, isovitexin, swertiamarin and amarogentin prevents proliferation of aortic smooth muscle cells in response to PDGF-BB. Cell proliferation and cell cycle analysis were performed based on alamar blue assay and propidium iodide labeling respectively. In primary cultures of rat aortic smooth muscle cells (RASMCs), PDGF-BB (20 ng/ml) induced a two-fold increase in cell proliferation which was significantly blocked by the root extract (1 mg/ml). The root extract also prevented the S-phase entry of synchronized cells in response to PDGF. Furthermore, PDGF-BB induced ERK1/2 activation and consequent increase in cellular nitric oxide (NO) levels were also blocked by the extract. These effects of extract were due to blockade of PDGF-BB induced expression of iNOS, cyclin D1 and proliferating cell nuclear antigen (PCNA). Docking analysis of the extract components on MEK1, the upstream ERK1/2 activating kinase using AutoDock4, indicated a likely binding of isovitexin to the inhibitor binding site of MEK1. Experiments performed with purified isovitexin demonstrated that it successfully blocks PDGF-induced ERK1/2 activation and proliferation of RASMCs in cell culture. Thus, Gentiana lutea can provide novel candidates for prevention and treatment of atherosclerosis.",
journal = "PLOS One",
title = "Inhibition of Vascular Smooth Muscle Cell Proliferation by Gentiana lutea Root Extracts",
volume = "8",
number = "4",
doi = "10.1371/journal.pone.0061393"
}

Kesavan, R., Potunuru, U. R., Nastasijević, B. J., Avaneesh, T., Joksić, G.,& Dixit, M.. (2013). Inhibition of Vascular Smooth Muscle Cell Proliferation by Gentiana lutea Root Extracts. in PLOS One, 8(4).
https://doi.org/10.1371/journal.pone.0061393

Kesavan R, Potunuru UR, Nastasijević BJ, Avaneesh T, Joksić G, Dixit M. Inhibition of Vascular Smooth Muscle Cell Proliferation by Gentiana lutea Root Extracts. in PLOS One. 2013;8(4).
doi:10.1371/journal.pone.0061393 .

Kesavan, Rushendhiran, Potunuru, Uma Rani, Nastasijević, Branislav J., Avaneesh, T., Joksić, Gordana, Dixit, Madhulika, "Inhibition of Vascular Smooth Muscle Cell Proliferation by Gentiana lutea Root Extracts" in PLOS One, 8, no. 4 (2013),
https://doi.org/10.1371/journal.pone.0061393 . .