TY  - JOUR
AU  - Keta, Otilija D.
AU  - Petković, Vladana
AU  - Cirrone, Pablo
AU  - Petringa, Giada
AU  - Cuttone, Giacomo
AU  - Sakata, Dousatsu
AU  - Shin, Wook-Geun
AU  - Incerti, Sebastien
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9875
AB  - Purpose The complex relationship between linear energy transfer (LET) and cellular response to radiation is not yet fully elucidated. To better characterize DNA damage after irradiations with therapeutic protons, we monitored formation and disappearance of DNA double-strand breaks (DNA DSB) as a function of LET and time. Comparisons with conventional γ-rays and high LET carbon ions were also performed.Materials and Methods In the present work, we performed immunofluorescence-based assay to determine the amount of DNA DSB induced by different LET values along the 62 MeV therapeutic proton Spread out Bragg peak (SOBP) in three cancer cell lines, i.e. HTB140 melanoma, MCF-7 breast adenocarcinoma and HTB177 non-small lung cancer cells. Time dependence of foci formation was followed as well. To determine irradiation positions, corresponding to the desired LET values, numerical simulations were carried out using Geant4 toolkit. We compared γ-H2AX foci persistence after irradiations with protons to that of γ-rays and carbon ions.Results With the rise of LET values along the therapeutic proton SOBP, the increase of γ-H2AX foci number is detected in the three cell lines up to the distal end of the SOBP, while there is a decrease on its distal fall-off part. With the prolonged incubation time, the number of foci gradually drops tending to attain the residual level. For the maximum number of DNA DSB, irradiation with protons attain higher level than that of γ-rays. Carbon ions produce more DNA DSB than protons but not substantially. The number of residual foci produced by γ-rays is significantly lower than that of protons and particularly carbon ions. Carbon ions do not produce considerably higher number of foci than protons, as it could be expected due to their physical properties.Conclusions In situ visualization of γ-H2AX foci reveal creation of more lesions in the three cell lines by clinically relevant proton SOBP than γ-rays. The lack of significant differences in the number of γ-H2AX foci between the proton and carbon ion-irradiated samples suggests an increased complexity of DNA lesions and slower repair kinetics after carbon ions compared to protons. For all three irradiation types, there is no major difference between the three cell lines shortly after irradiations, while later on, the formation of residual foci starts to express the inherent nature of tested cells, therefore increasing discrepancy between them.
T2  - International Journal of Radiation Biology
T1  - DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time
VL  - 97
IS  - 9
SP  - 1229
EP  - 1240
DO  - 10.1080/09553002.2021.1948140
ER  - 

@article{
author = "Keta, Otilija D. and Petković, Vladana and Cirrone, Pablo and Petringa, Giada and Cuttone, Giacomo and Sakata, Dousatsu and Shin, Wook-Geun and Incerti, Sebastien and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2021",
abstract = "Purpose The complex relationship between linear energy transfer (LET) and cellular response to radiation is not yet fully elucidated. To better characterize DNA damage after irradiations with therapeutic protons, we monitored formation and disappearance of DNA double-strand breaks (DNA DSB) as a function of LET and time. Comparisons with conventional γ-rays and high LET carbon ions were also performed.Materials and Methods In the present work, we performed immunofluorescence-based assay to determine the amount of DNA DSB induced by different LET values along the 62 MeV therapeutic proton Spread out Bragg peak (SOBP) in three cancer cell lines, i.e. HTB140 melanoma, MCF-7 breast adenocarcinoma and HTB177 non-small lung cancer cells. Time dependence of foci formation was followed as well. To determine irradiation positions, corresponding to the desired LET values, numerical simulations were carried out using Geant4 toolkit. We compared γ-H2AX foci persistence after irradiations with protons to that of γ-rays and carbon ions.Results With the rise of LET values along the therapeutic proton SOBP, the increase of γ-H2AX foci number is detected in the three cell lines up to the distal end of the SOBP, while there is a decrease on its distal fall-off part. With the prolonged incubation time, the number of foci gradually drops tending to attain the residual level. For the maximum number of DNA DSB, irradiation with protons attain higher level than that of γ-rays. Carbon ions produce more DNA DSB than protons but not substantially. The number of residual foci produced by γ-rays is significantly lower than that of protons and particularly carbon ions. Carbon ions do not produce considerably higher number of foci than protons, as it could be expected due to their physical properties.Conclusions In situ visualization of γ-H2AX foci reveal creation of more lesions in the three cell lines by clinically relevant proton SOBP than γ-rays. The lack of significant differences in the number of γ-H2AX foci between the proton and carbon ion-irradiated samples suggests an increased complexity of DNA lesions and slower repair kinetics after carbon ions compared to protons. For all three irradiation types, there is no major difference between the three cell lines shortly after irradiations, while later on, the formation of residual foci starts to express the inherent nature of tested cells, therefore increasing discrepancy between them.",
journal = "International Journal of Radiation Biology",
title = "DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time",
volume = "97",
number = "9",
pages = "1229-1240",
doi = "10.1080/09553002.2021.1948140"
}

Keta, O. D., Petković, V., Cirrone, P., Petringa, G., Cuttone, G., Sakata, D., Shin, W., Incerti, S., Petrović, I. M.,& Ristić-Fira, A.. (2021). DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time. in International Journal of Radiation Biology, 97(9), 1229-1240.
https://doi.org/10.1080/09553002.2021.1948140

Keta OD, Petković V, Cirrone P, Petringa G, Cuttone G, Sakata D, Shin W, Incerti S, Petrović IM, Ristić-Fira A. DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time. in International Journal of Radiation Biology. 2021;97(9):1229-1240.
doi:10.1080/09553002.2021.1948140 .

Keta, Otilija D., Petković, Vladana, Cirrone, Pablo, Petringa, Giada, Cuttone, Giacomo, Sakata, Dousatsu, Shin, Wook-Geun, Incerti, Sebastien, Petrović, Ivan M., Ristić-Fira, Aleksandra, "DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time" in International Journal of Radiation Biology, 97, no. 9 (2021):1229-1240,
https://doi.org/10.1080/09553002.2021.1948140 . .

TY  - JOUR
AU  - Starčević, Nikica
AU  - Petrović, Srđan M.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9797
AB  - In this work we show a way how the crystal rainbow channeling effect can be applied for obtaining the interaction potential between 2 MeV protons and the cubic crystallographic crystals, in the cases of axial channeling of protons along (0 0 1) and (1 1 1) crystal directions. The procedure for construction of this potential has been based on our previous work in which the rainbow morphology was crucial for their determination. However, important improvements have been made. As a result, the two axial channeling directions can be treated using the same physical arguments leading to more consistent values of the fitting parameters in the ion-atom interaction potential.
T2  - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
T1  - Crystal rainbow channeling potential for 〈1 0 0〉 and 〈1 1 1〉 cubic crystallographic crystals
VL  - 499
SP  - 39
EP  - 45
DO  - 10.1016/j.nimb.2021.03.004
ER  - 

@article{
author = "Starčević, Nikica and Petrović, Srđan M.",
year = "2021",
abstract = "In this work we show a way how the crystal rainbow channeling effect can be applied for obtaining the interaction potential between 2 MeV protons and the cubic crystallographic crystals, in the cases of axial channeling of protons along (0 0 1) and (1 1 1) crystal directions. The procedure for construction of this potential has been based on our previous work in which the rainbow morphology was crucial for their determination. However, important improvements have been made. As a result, the two axial channeling directions can be treated using the same physical arguments leading to more consistent values of the fitting parameters in the ion-atom interaction potential.",
journal = "Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms",
title = "Crystal rainbow channeling potential for 〈1 0 0〉 and 〈1 1 1〉 cubic crystallographic crystals",
volume = "499",
pages = "39-45",
doi = "10.1016/j.nimb.2021.03.004"
}

Starčević, N.,& Petrović, S. M.. (2021). Crystal rainbow channeling potential for 〈1 0 0〉 and 〈1 1 1〉 cubic crystallographic crystals. in Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, 499, 39-45.
https://doi.org/10.1016/j.nimb.2021.03.004

Starčević N, Petrović SM. Crystal rainbow channeling potential for 〈1 0 0〉 and 〈1 1 1〉 cubic crystallographic crystals. in Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms. 2021;499:39-45.
doi:10.1016/j.nimb.2021.03.004 .

Starčević, Nikica, Petrović, Srđan M., "Crystal rainbow channeling potential for 〈1 0 0〉 and 〈1 1 1〉 cubic crystallographic crystals" in Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, 499 (2021):39-45,
https://doi.org/10.1016/j.nimb.2021.03.004 . .

TY  - JOUR
AU  - Matović, Branko
AU  - Prekajski, Marija D.
AU  - Pantić, Jelena R.
AU  - Braeuniger, Thomas
AU  - Rosić, Milena
AU  - Zagorac, Dejan
AU  - Milivojević, Dušan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1227
AB  - Mayenite (C12A7) powders have been synthesized by self-combustion method (glycine/nitrate procedure - GNP and a newly suggested modified glycine/nitrate procedure - MGNP). The MGNP is derived from the GNP by substituting a portion of Al-nitrate with the less expensive Al-acetate. The effect of synthesis conditions of both procedures on the phase evolution was studied. Mayenite samples, obtained by using GNP and MGNP, followed by thermal treatment in air, were analyzed by using XRD method. The obtained results showed that crystallization started at 900 degrees C for the GNP and at 750 degrees C for the MGNP while below these temperatures samples are amorphous. Also, only observed phase was mayenite for MGNP, while a secondary phase is present in samples synthesized by using GNP. Scanning electron microscopy (SEM), electron paramagnetic resonance (EPR) and nuclear magnetic resonance spectroscopy (NMR) were used to characterize the pure mayenite phase at room temperature. Sintering behavior was studied by dilatometry. A relative density of 94% is obtained at 1300 degrees C. (C) 2016 Elsevier Ltd. All rights reserved.
T2  - Journal of the European Ceramic Society
T1  - Synthesis and densification of single-phase mayenite (C12A7)
VL  - 36
IS  - 16
SP  - 4237
EP  - 4241
DO  - 10.1016/j.jeurceramsoc.2016.06.014
ER  - 

@article{
author = "Matović, Branko and Prekajski, Marija D. and Pantić, Jelena R. and Braeuniger, Thomas and Rosić, Milena and Zagorac, Dejan and Milivojević, Dušan",
year = "2016",
abstract = "Mayenite (C12A7) powders have been synthesized by self-combustion method (glycine/nitrate procedure - GNP and a newly suggested modified glycine/nitrate procedure - MGNP). The MGNP is derived from the GNP by substituting a portion of Al-nitrate with the less expensive Al-acetate. The effect of synthesis conditions of both procedures on the phase evolution was studied. Mayenite samples, obtained by using GNP and MGNP, followed by thermal treatment in air, were analyzed by using XRD method. The obtained results showed that crystallization started at 900 degrees C for the GNP and at 750 degrees C for the MGNP while below these temperatures samples are amorphous. Also, only observed phase was mayenite for MGNP, while a secondary phase is present in samples synthesized by using GNP. Scanning electron microscopy (SEM), electron paramagnetic resonance (EPR) and nuclear magnetic resonance spectroscopy (NMR) were used to characterize the pure mayenite phase at room temperature. Sintering behavior was studied by dilatometry. A relative density of 94% is obtained at 1300 degrees C. (C) 2016 Elsevier Ltd. All rights reserved.",
journal = "Journal of the European Ceramic Society",
title = "Synthesis and densification of single-phase mayenite (C12A7)",
volume = "36",
number = "16",
pages = "4237-4241",
doi = "10.1016/j.jeurceramsoc.2016.06.014"
}

Matović, B., Prekajski, M. D., Pantić, J. R., Braeuniger, T., Rosić, M., Zagorac, D.,& Milivojević, D.. (2016). Synthesis and densification of single-phase mayenite (C12A7). in Journal of the European Ceramic Society, 36(16), 4237-4241.
https://doi.org/10.1016/j.jeurceramsoc.2016.06.014

Matović B, Prekajski MD, Pantić JR, Braeuniger T, Rosić M, Zagorac D, Milivojević D. Synthesis and densification of single-phase mayenite (C12A7). in Journal of the European Ceramic Society. 2016;36(16):4237-4241.
doi:10.1016/j.jeurceramsoc.2016.06.014 .

Matović, Branko, Prekajski, Marija D., Pantić, Jelena R., Braeuniger, Thomas, Rosić, Milena, Zagorac, Dejan, Milivojević, Dušan, "Synthesis and densification of single-phase mayenite (C12A7)" in Journal of the European Ceramic Society, 36, no. 16 (2016):4237-4241,
https://doi.org/10.1016/j.jeurceramsoc.2016.06.014 . .

TY  - JOUR
AU  - Paprika, Milijana
AU  - Komatina, Mirko
AU  - Dakić, Dragoljub V.
AU  - Živković, Goran S.
AU  - Mladenović, Milica R.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7069
AB  - The paper presents a comparison between experimental and model results of primary fragmentation of a lignite coal in a fluidized bed (FB). In the experiments, the char particle size distribution and the general indicators of primary fragmentation (intensity and index) were determined. The same parameters were calculated using a mathematical model of the process, fed by data of the fuel (the amount of volatiles and fixed carbon), fluidized bed temperature; and inlet particle size distribution. The size distribution and number of the char particles in fluidized bed significantly differ from the size distribution and number of inlet coal particles. Char population has a bimodal distribution separate distributions for the smaller and larger sets of fragments. The experimental and model results show the same tendency: a coal particle partially breaks at the beginning of devolatilization, giving a large number of fine fragments, while, as the process continues, the rest of the parent particle sometimes breaks down into a smaller number of larger pieces, and sometimes does not fragment at all. Review of the Weibull distribution coefficients enables prediction of the char particle size distribution for the characteristic fluidized bed conditions and inlet coal particle sizes.
T2  - Energy and Fuels
T1  - Experimental and Numerical Investigation of the Primary Fragmentation of a Lignite during Fluidized-Bed (FB) Devolatilization
VL  - 29
IS  - 5
SP  - 3394
EP  - 3398
DO  - 10.1021/ef5024803
ER  - 

@article{
author = "Paprika, Milijana and Komatina, Mirko and Dakić, Dragoljub V. and Živković, Goran S. and Mladenović, Milica R.",
year = "2015",
abstract = "The paper presents a comparison between experimental and model results of primary fragmentation of a lignite coal in a fluidized bed (FB). In the experiments, the char particle size distribution and the general indicators of primary fragmentation (intensity and index) were determined. The same parameters were calculated using a mathematical model of the process, fed by data of the fuel (the amount of volatiles and fixed carbon), fluidized bed temperature; and inlet particle size distribution. The size distribution and number of the char particles in fluidized bed significantly differ from the size distribution and number of inlet coal particles. Char population has a bimodal distribution separate distributions for the smaller and larger sets of fragments. The experimental and model results show the same tendency: a coal particle partially breaks at the beginning of devolatilization, giving a large number of fine fragments, while, as the process continues, the rest of the parent particle sometimes breaks down into a smaller number of larger pieces, and sometimes does not fragment at all. Review of the Weibull distribution coefficients enables prediction of the char particle size distribution for the characteristic fluidized bed conditions and inlet coal particle sizes.",
journal = "Energy and Fuels",
title = "Experimental and Numerical Investigation of the Primary Fragmentation of a Lignite during Fluidized-Bed (FB) Devolatilization",
volume = "29",
number = "5",
pages = "3394-3398",
doi = "10.1021/ef5024803"
}

Paprika, M., Komatina, M., Dakić, D. V., Živković, G. S.,& Mladenović, M. R.. (2015). Experimental and Numerical Investigation of the Primary Fragmentation of a Lignite during Fluidized-Bed (FB) Devolatilization. in Energy and Fuels, 29(5), 3394-3398.
https://doi.org/10.1021/ef5024803

Paprika M, Komatina M, Dakić DV, Živković GS, Mladenović MR. Experimental and Numerical Investigation of the Primary Fragmentation of a Lignite during Fluidized-Bed (FB) Devolatilization. in Energy and Fuels. 2015;29(5):3394-3398.
doi:10.1021/ef5024803 .

Paprika, Milijana, Komatina, Mirko, Dakić, Dragoljub V., Živković, Goran S., Mladenović, Milica R., "Experimental and Numerical Investigation of the Primary Fragmentation of a Lignite during Fluidized-Bed (FB) Devolatilization" in Energy and Fuels, 29, no. 5 (2015):3394-3398,
https://doi.org/10.1021/ef5024803 . .