TY  - JOUR
AU  - Simonović, Mladen
AU  - Ostojić, Sanja
AU  - Micić, Darko
AU  - Savić-Biserčić, Marjetka
AU  - Mix, Thorsten
AU  - Glumac, Miodrag
AU  - Pejin, Boris
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10665
AB  - This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] .
T2  - Natural Product Research
T1  - A novel and effective natural product-based immunodetection tool for TNT-like compounds
VL  - 36
IS  - 3
SP  - 857
EP  - 861
DO  - 10.1080/14786419.2020.1806269
ER  - 

@article{
author = "Simonović, Mladen and Ostojić, Sanja and Micić, Darko and Savić-Biserčić, Marjetka and Mix, Thorsten and Glumac, Miodrag and Pejin, Boris",
year = "2022",
abstract = "This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] .",
journal = "Natural Product Research",
title = "A novel and effective natural product-based immunodetection tool for TNT-like compounds",
volume = "36",
number = "3",
pages = "857-861",
doi = "10.1080/14786419.2020.1806269"
}

Simonović, M., Ostojić, S., Micić, D., Savić-Biserčić, M., Mix, T., Glumac, M.,& Pejin, B.. (2022). A novel and effective natural product-based immunodetection tool for TNT-like compounds. in Natural Product Research, 36(3), 857-861.
https://doi.org/10.1080/14786419.2020.1806269

Simonović M, Ostojić S, Micić D, Savić-Biserčić M, Mix T, Glumac M, Pejin B. A novel and effective natural product-based immunodetection tool for TNT-like compounds. in Natural Product Research. 2022;36(3):857-861.
doi:10.1080/14786419.2020.1806269 .

Simonović, Mladen, Ostojić, Sanja, Micić, Darko, Savić-Biserčić, Marjetka, Mix, Thorsten, Glumac, Miodrag, Pejin, Boris, "A novel and effective natural product-based immunodetection tool for TNT-like compounds" in Natural Product Research, 36, no. 3 (2022):857-861,
https://doi.org/10.1080/14786419.2020.1806269 . .

TY  - JOUR
AU  - Nešić, Aleksandra
AU  - Ružić, Jovana
AU  - Gordić, Milan V.
AU  - Ostojić, Sanja 
AU  - Micić, Darko M.
AU  - Onjia, Antonije E.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1446
AB  - In this work, the new biodegradable blend films composed of natural polymer pectin and synthetic polymer polyvinylpyrrolidone were obtained through casting process. The concentration of glycerol in all formulations was constant (2% v/v), while the amount of polyvinylpyrrolidone varied from 20 to 60% by mass of pectin. The molecular interactions between two components were elucidated by FTIR-ATR spectroscopy, while functional properties of these films were carried out by determination of puncture resistance, tensile strength, thermal stability, water vapor barrier, wettability and printability. The blending of pectin with PVP led to an improvement of mechanical resistance, barrier properties and hydrophobicity of these films for 57%, 58 and 24%, respectively, remaining adequate thermal stability. Since pectin and PVP are nontoxic, food-grade and biodegradable materials, this research present an ecosustainable approach to provide films with satisfied physical properties for use as packaging material of non-food products, such as water-treatment products (detergents, biocides, agrochemical compounds). (C) 2016 Elsevier Ltd. All rights reserved.
T2  - Composites: Part B: Engineering
T1  - Pectin-polyvinylpyrrolidone films: A sustainable approach to the development of biobased packaging materials
VL  - 110
SP  - 56
EP  - 61
DO  - 10.1016/j.compositesb.2016.11.016
ER  - 

@article{
author = "Nešić, Aleksandra and Ružić, Jovana and Gordić, Milan V. and Ostojić, Sanja  and Micić, Darko M. and Onjia, Antonije E.",
year = "2017",
abstract = "In this work, the new biodegradable blend films composed of natural polymer pectin and synthetic polymer polyvinylpyrrolidone were obtained through casting process. The concentration of glycerol in all formulations was constant (2% v/v), while the amount of polyvinylpyrrolidone varied from 20 to 60% by mass of pectin. The molecular interactions between two components were elucidated by FTIR-ATR spectroscopy, while functional properties of these films were carried out by determination of puncture resistance, tensile strength, thermal stability, water vapor barrier, wettability and printability. The blending of pectin with PVP led to an improvement of mechanical resistance, barrier properties and hydrophobicity of these films for 57%, 58 and 24%, respectively, remaining adequate thermal stability. Since pectin and PVP are nontoxic, food-grade and biodegradable materials, this research present an ecosustainable approach to provide films with satisfied physical properties for use as packaging material of non-food products, such as water-treatment products (detergents, biocides, agrochemical compounds). (C) 2016 Elsevier Ltd. All rights reserved.",
journal = "Composites: Part B: Engineering",
title = "Pectin-polyvinylpyrrolidone films: A sustainable approach to the development of biobased packaging materials",
volume = "110",
pages = "56-61",
doi = "10.1016/j.compositesb.2016.11.016"
}

Nešić, A., Ružić, J., Gordić, M. V., Ostojić, S., Micić, D. M.,& Onjia, A. E.. (2017). Pectin-polyvinylpyrrolidone films: A sustainable approach to the development of biobased packaging materials. in Composites: Part B: Engineering, 110, 56-61.
https://doi.org/10.1016/j.compositesb.2016.11.016

Nešić A, Ružić J, Gordić MV, Ostojić S, Micić DM, Onjia AE. Pectin-polyvinylpyrrolidone films: A sustainable approach to the development of biobased packaging materials. in Composites: Part B: Engineering. 2017;110:56-61.
doi:10.1016/j.compositesb.2016.11.016 .

Nešić, Aleksandra, Ružić, Jovana, Gordić, Milan V., Ostojić, Sanja , Micić, Darko M., Onjia, Antonije E., "Pectin-polyvinylpyrrolidone films: A sustainable approach to the development of biobased packaging materials" in Composites: Part B: Engineering, 110 (2017):56-61,
https://doi.org/10.1016/j.compositesb.2016.11.016 . .