TY  - JOUR
AU  - Paraš, Smiljana
AU  - Trišić, Dijana
AU  - Mitrović Ajtić, Olivera
AU  - Prokić, Bogomir
AU  - Drobne, Damjana
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman R.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9008
AB  - Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.
T2  - Nanomaterials
T1  - Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats
VL  - 10
IS  - 5
SP  - 918
DO  - 10.3390/nano10050918
ER  - 

@article{
author = "Paraš, Smiljana and Trišić, Dijana and Mitrović Ajtić, Olivera and Prokić, Bogomir and Drobne, Damjana and Živković, Slavoljub and Jokanović, Vukoman R.",
year = "2020",
abstract = "Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.",
journal = "Nanomaterials",
title = "Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats",
volume = "10",
number = "5",
pages = "918",
doi = "10.3390/nano10050918"
}

Paraš, S., Trišić, D., Mitrović Ajtić, O., Prokić, B., Drobne, D., Živković, S.,& Jokanović, V. R.. (2020). Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats. in Nanomaterials, 10(5), 918.
https://doi.org/10.3390/nano10050918

Paraš S, Trišić D, Mitrović Ajtić O, Prokić B, Drobne D, Živković S, Jokanović VR. Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats. in Nanomaterials. 2020;10(5):918.
doi:10.3390/nano10050918 .

Paraš, Smiljana, Trišić, Dijana, Mitrović Ajtić, Olivera, Prokić, Bogomir, Drobne, Damjana, Živković, Slavoljub, Jokanović, Vukoman R., "Toxicological Profile of Nanostructured Bone Substitute Based on Hydroxyapatite and Poly(lactide-co-glycolide) after Subchronic Oral Exposure of Rats" in Nanomaterials, 10, no. 5 (2020):918,
https://doi.org/10.3390/nano10050918 . .