TY  - CONF
AU  - Ralić, Vanja
AU  - Gemović, Branislava
AU  - Nešić, Maja D.
AU  - Popović, Iva
AU  - Stepić, Milutin
AU  - Petković, Marijana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12390
AB  - In this work, we have applied the Informational Spectrum Method (ISM) to discover a potential protein target in the HeLa cervical cancer cell line for [Pd(dach)Cl2] complex. Since Pd complexes are considered an alternative to traditionally used Pt complexes in anti-cancer therapy, it is essential to elucidate the mechanism of their action. A detailed analysis that also involves screening the known protein databases revealed the proteins of the SOSS complex as the most probable [Pd(dach)Cl2] targets. Since this protein maintains genomic stability, this result shows the potential of the Pd(II) complex as an anti-cancer drug.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells
SP  - 296
EP  - 299
DO  - 10.46793/ICCBI23.296R
ER  - 

@conference{
author = "Ralić, Vanja and Gemović, Branislava and Nešić, Maja D. and Popović, Iva and Stepić, Milutin and Petković, Marijana",
year = "2023",
abstract = "In this work, we have applied the Informational Spectrum Method (ISM) to discover a potential protein target in the HeLa cervical cancer cell line for [Pd(dach)Cl2] complex. Since Pd complexes are considered an alternative to traditionally used Pt complexes in anti-cancer therapy, it is essential to elucidate the mechanism of their action. A detailed analysis that also involves screening the known protein databases revealed the proteins of the SOSS complex as the most probable [Pd(dach)Cl2] targets. Since this protein maintains genomic stability, this result shows the potential of the Pd(II) complex as an anti-cancer drug.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells",
pages = "296-299",
doi = "10.46793/ICCBI23.296R"
}

Ralić, V., Gemović, B., Nešić, M. D., Popović, I., Stepić, M.,& Petković, M.. (2023). Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 296-299.
https://doi.org/10.46793/ICCBI23.296R

Ralić V, Gemović B, Nešić MD, Popović I, Stepić M, Petković M. Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:296-299.
doi:10.46793/ICCBI23.296R .

Ralić, Vanja, Gemović, Branislava, Nešić, Maja D., Popović, Iva, Stepić, Milutin, Petković, Marijana, "Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):296-299,
https://doi.org/10.46793/ICCBI23.296R . .

TY  - CONF
AU  - Ralić, Vanja
AU  - Nešić, Maja
AU  - Abu el Rub, Anamarija
AU  - Dučić, Tanja
AU  - Gemović, Branislava
AU  - Algarra, Manuel
AU  - Stepić, Milutin
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Petković, Marijana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12657
AB  - Nanocomposite system formulated from surface-modified S-doped carbon dot (S-CD) nanoparticle with a potential metallodrug, palladium(II) complex, dichloro(1,2-diaminocyclohexane)palladium(II), [Pd(dach)Cl2] (Pd@S-CD), was investigated as a model system for the treatment of cervical carcinoma (HeLa) cells. To examine the intracellular biochemical effects induced by the Pd@S-CD, we used Synchrotron Radiation-based Fourier-transform infrared spectroscopy (SR FTIR). SR FTIR spectroscopy was employed to investigate the alterations in cellular components’ biochemical composition and secondary structure upon exposure to Pd@S-CD. Spectral analysis, complemented by statistical techniques, revealed changes in biomolecules, lipids, proteins, nucleic acids, and carbohydrates caused by the treatment with Pd@CDs. These results and the increased cytotoxicity of the system demonstrate its high anti-cervical cancer therapeutic potential.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells
SP  - 467
EP  - 470
DO  - 10.46793/ICCBI23.467R
ER  - 

@conference{
author = "Ralić, Vanja and Nešić, Maja and Abu el Rub, Anamarija and Dučić, Tanja and Gemović, Branislava and Algarra, Manuel and Stepić, Milutin and Korićanac, Lela and Žakula, Jelena and Petković, Marijana",
year = "2023",
abstract = "Nanocomposite system formulated from surface-modified S-doped carbon dot (S-CD) nanoparticle with a potential metallodrug, palladium(II) complex, dichloro(1,2-diaminocyclohexane)palladium(II), [Pd(dach)Cl2] (Pd@S-CD), was investigated as a model system for the treatment of cervical carcinoma (HeLa) cells. To examine the intracellular biochemical effects induced by the Pd@S-CD, we used Synchrotron Radiation-based Fourier-transform infrared spectroscopy (SR FTIR). SR FTIR spectroscopy was employed to investigate the alterations in cellular components’ biochemical composition and secondary structure upon exposure to Pd@S-CD. Spectral analysis, complemented by statistical techniques, revealed changes in biomolecules, lipids, proteins, nucleic acids, and carbohydrates caused by the treatment with Pd@CDs. These results and the increased cytotoxicity of the system demonstrate its high anti-cervical cancer therapeutic potential.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells",
pages = "467-470",
doi = "10.46793/ICCBI23.467R"
}

Ralić, V., Nešić, M., Abu el Rub, A., Dučić, T., Gemović, B., Algarra, M., Stepić, M., Korićanac, L., Žakula, J.,& Petković, M.. (2023). SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 467-470.
https://doi.org/10.46793/ICCBI23.467R

Ralić V, Nešić M, Abu el Rub A, Dučić T, Gemović B, Algarra M, Stepić M, Korićanac L, Žakula J, Petković M. SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:467-470.
doi:10.46793/ICCBI23.467R .

Ralić, Vanja, Nešić, Maja, Abu el Rub, Anamarija, Dučić, Tanja, Gemović, Branislava, Algarra, Manuel, Stepić, Milutin, Korićanac, Lela, Žakula, Jelena, Petković, Marijana, "SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):467-470,
https://doi.org/10.46793/ICCBI23.467R . .

TY  - JOUR
AU  - Šokarda Slavić, Marinela
AU  - Ralić, Vanja
AU  - Nastasijević, Branislav
AU  - Matijević, Milica
AU  - Vujčić, Zoran
AU  - Margetić, Aleksandra
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11988
AB  - Poly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible, and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box-Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS-PAGE followed by Methylene Blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. Encouraging, PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone.
AB  - Поли(γ-глутаминска киселина) (ПГK), коју производе бактерије рода Bacillus, је биоразградив, нетоксичан, биокомпатибилан и неимуноген негативно наелектрисани полимер. Због својих својстава нашао је разноврсну примену у прехрамбеној, козметичкој и фармацеутској индустрији. У овом раду, Bacillus ѕubtilis 17Б је изабран као најбољи ПГК продуцер међу педесетак тестираних природних изолата бактерија из овог рода и окарактерисан као глутамат независтан продуцер. Производња ПГК овим новоидентификованим сојем је оптимизована и десетоструко увећана коришћењем Box-Behnken експерименталног дизајна. Чистоћа ПГК након изоловања и пречишћавања из ферметационе течности је потврђена електрофорезом (SDS-PAGE) након бојења метиленским плавим. ПГК је окарактерисана масеном спекроскопијом (ESI MS) и коришћена за добијање новог нанокомпозита са ТiО2. Синтеза ПГК/ТiО2 нанокомпозита, његова структурна анализа и цитотоксични ефекат на ћелијску линију рака грлића материце (HeLa ћелије) је испитан да би се утврдила потенцијална употреба овог новодобијеног материјала у борби против ћелија рака. Нанокомпозит ПГК/ТiО2показао је повећан цитотоксични ефекат на поменуте ћелије рака у поређењу са самим ТiО2.
T2  - Journal of the Serbian Chemical Society
T1  - A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain
T1  - Нови ПГК/TiO2 нанокомпозит добијен од поли(γ -глутаминске киселине) из новоизолованог соја bacillus subtilis 17B
VL  - 88
IS  - 10
SP  - 985
EP  - 997
DO  - 10.2298/JSC221116011S
ER  - 

@article{
author = "Šokarda Slavić, Marinela and Ralić, Vanja and Nastasijević, Branislav and Matijević, Milica and Vujčić, Zoran and Margetić, Aleksandra",
year = "2023",
abstract = "Poly(γ-glutamic acid) (PGA), naturally produced by Bacillus species, is a biodegradable, non-toxic, biocompatible, and non-immunogenic negatively charged polymer. Due to its properties, it has found various applications in the food, cosmetic and pharmaceutical industries. In this work, Bacillus subtilis 17B was selected as the best PGA producer among fifty wild-types Bacillus strains tested and characterized as a glutamate-independent producer. The production of PGA by the newly identified strain was optimized and increased tenfold using the Box-Behnken experimental design. The purity of PGA after recovery and purification from the fermentation broth was confirmed by SDS-PAGE followed by Methylene Blue staining. PGA was characterized by ESI MS and used for the preparation of a new nanocomposite with TiO2. The synthesis of PGA/TiO2 nanocomposite, its structural analysis, and cytotoxic effect on the cervical cancer cell line (HeLa cell) was investigated to determine the potential anti-cancer usage of this newly prepared material. Encouraging, PGA/TiO2 nanocomposite showed an increased cytotoxic effect compared to TiO2 alone., Поли(γ-глутаминска киселина) (ПГK), коју производе бактерије рода Bacillus, је биоразградив, нетоксичан, биокомпатибилан и неимуноген негативно наелектрисани полимер. Због својих својстава нашао је разноврсну примену у прехрамбеној, козметичкој и фармацеутској индустрији. У овом раду, Bacillus ѕubtilis 17Б је изабран као најбољи ПГК продуцер међу педесетак тестираних природних изолата бактерија из овог рода и окарактерисан као глутамат независтан продуцер. Производња ПГК овим новоидентификованим сојем је оптимизована и десетоструко увећана коришћењем Box-Behnken експерименталног дизајна. Чистоћа ПГК након изоловања и пречишћавања из ферметационе течности је потврђена електрофорезом (SDS-PAGE) након бојења метиленским плавим. ПГК је окарактерисана масеном спекроскопијом (ESI MS) и коришћена за добијање новог нанокомпозита са ТiО2. Синтеза ПГК/ТiО2 нанокомпозита, његова структурна анализа и цитотоксични ефекат на ћелијску линију рака грлића материце (HeLa ћелије) је испитан да би се утврдила потенцијална употреба овог новодобијеног материјала у борби против ћелија рака. Нанокомпозит ПГК/ТiО2показао је повећан цитотоксични ефекат на поменуте ћелије рака у поређењу са самим ТiО2.",
journal = "Journal of the Serbian Chemical Society",
title = "A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain, Нови ПГК/TiO2 нанокомпозит добијен од поли(γ -глутаминске киселине) из новоизолованог соја bacillus subtilis 17B",
volume = "88",
number = "10",
pages = "985-997",
doi = "10.2298/JSC221116011S"
}

Šokarda Slavić, M., Ralić, V., Nastasijević, B., Matijević, M., Vujčić, Z.,& Margetić, A.. (2023). A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain. in Journal of the Serbian Chemical Society, 88(10), 985-997.
https://doi.org/10.2298/JSC221116011S

Šokarda Slavić M, Ralić V, Nastasijević B, Matijević M, Vujčić Z, Margetić A. A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain. in Journal of the Serbian Chemical Society. 2023;88(10):985-997.
doi:10.2298/JSC221116011S .

Šokarda Slavić, Marinela, Ralić, Vanja, Nastasijević, Branislav, Matijević, Milica, Vujčić, Zoran, Margetić, Aleksandra, "A novel PGA/TiO2 nanocomposite prepared with poly(γ-glutamic acid) from the newly isolated Bacillus subtilis 17B strain" in Journal of the Serbian Chemical Society, 88, no. 10 (2023):985-997,
https://doi.org/10.2298/JSC221116011S . .