TY  - CONF
AU  - Cvetković, S
AU  - Nastasijević, Branislav J.
AU  - Mitić-Ćulafić, D
AU  - Đukanović, S
AU  - Nikolić, B
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10057
AB  - Lipid oxidation that occurs in different types of food can cause alterations in nutritional qualities, flavour, texture and shelf life of foods. Furthermore, high temperature cooking of protein-rich food can lead to formation of heterocyclic aromatic amines capable of compromising the integrity of DNA molecules. To reduce these harmful effects, research has been focused on investigating plants as a source of potential natural food additives and preservatives. Thus, the aim of this study was to estimate antioxidant and antigenotoxic activities of 50% ethanolic-aqueous root and leaf extracts of the medicinal plant, Gentiana lutea . Antioxidative effect was investigated using the DPPH assay, while antigenotoxicity against the mutagen 2-amino-1-methyl-6-phenylimidazo[4,5- b ]pyridine (PhIP) was determined using Salmonella Typhimurium TA 1535 in the SOS/ umuC assay. Leaf extract showed high antioxidative effect with the ability to neutralize up to 87% of free radicals at 400 µg mL -1 . Antigenotoxicity testing revealed that both extracts exhibited remarkable genoprotective activity against PhIP-induced DNA damage, with the highest inhibition levels being 70% and 85% for root and leaf extracts, respectively. Results obtained are encouraging and suggest further research of G. lutea extracts as potential food preservatives and additives in improving food quality and human health.
C3  - IOP Conference Series: Earth and Environmental Science
T1  - Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP
VL  - 854
IS  - 1
SP  - 012018
DO  - 10.1088/1755-1315/854/1/012018
ER  - 

@conference{
author = "Cvetković, S and Nastasijević, Branislav J. and Mitić-Ćulafić, D and Đukanović, S and Nikolić, B",
year = "2021",
abstract = "Lipid oxidation that occurs in different types of food can cause alterations in nutritional qualities, flavour, texture and shelf life of foods. Furthermore, high temperature cooking of protein-rich food can lead to formation of heterocyclic aromatic amines capable of compromising the integrity of DNA molecules. To reduce these harmful effects, research has been focused on investigating plants as a source of potential natural food additives and preservatives. Thus, the aim of this study was to estimate antioxidant and antigenotoxic activities of 50% ethanolic-aqueous root and leaf extracts of the medicinal plant, Gentiana lutea . Antioxidative effect was investigated using the DPPH assay, while antigenotoxicity against the mutagen 2-amino-1-methyl-6-phenylimidazo[4,5- b ]pyridine (PhIP) was determined using Salmonella Typhimurium TA 1535 in the SOS/ umuC assay. Leaf extract showed high antioxidative effect with the ability to neutralize up to 87% of free radicals at 400 µg mL -1 . Antigenotoxicity testing revealed that both extracts exhibited remarkable genoprotective activity against PhIP-induced DNA damage, with the highest inhibition levels being 70% and 85% for root and leaf extracts, respectively. Results obtained are encouraging and suggest further research of G. lutea extracts as potential food preservatives and additives in improving food quality and human health.",
journal = "IOP Conference Series: Earth and Environmental Science",
title = "Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP",
volume = "854",
number = "1",
pages = "012018",
doi = "10.1088/1755-1315/854/1/012018"
}

Cvetković, S., Nastasijević, B. J., Mitić-Ćulafić, D., Đukanović, S.,& Nikolić, B.. (2021). Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP. in IOP Conference Series: Earth and Environmental Science, 854(1), 012018.
https://doi.org/10.1088/1755-1315/854/1/012018

Cvetković S, Nastasijević BJ, Mitić-Ćulafić D, Đukanović S, Nikolić B. Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP. in IOP Conference Series: Earth and Environmental Science. 2021;854(1):012018.
doi:10.1088/1755-1315/854/1/012018 .

Cvetković, S, Nastasijević, Branislav J., Mitić-Ćulafić, D, Đukanović, S, Nikolić, B, "Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP" in IOP Conference Series: Earth and Environmental Science, 854, no. 1 (2021):012018,
https://doi.org/10.1088/1755-1315/854/1/012018 . .