TY  - CONF
AU  - Vučić, Vesna
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Tišma, Nevena
AU  - Janković, Dragana
AU  - Ruždijić, Sabera
AU  - Radojčić, Marija B.
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12038
AB  - Background: Prostate cancer is the first as an incidence and the second as a cause of the oncologic mortality in the male population. There is a broad range of possibilities in the prostate cancer therapy. However, there is also much controversy on the most appropriate treatment in the various stages of the disease. Advanced disease is mostly treated by radiation therapy, sometimes in combination with hormone or chemotherapy. Irradiation induces damage of cell biomolecules, which can lead to the arrest in cell division, or to apoptotic or necrotic cell death. The aim of this study was to determine the dose dependence of radiation-induced cell death in two human prostate cancer cell lines, and to define the form of death of these cells. Methods: Human prostate cancer cell lines PC-3 and DU-145 were irradiated with 2 - 30 Gy from 60 Co g-source, at the dose rate of 20 Gy/h. The effect of irradiation on cell viability, morphology and DNA structure were followed 24 - 72 hours after treatment. Cells were analyzed by trypan blue exclusion assay, flow cytometry and DNA gel electrophoresis. Simultaneous staining of cells with Annexin V-FITC and propidium iodide enabled distinction of early apoptosis from late apoptosis and/or necrosis. Results: The results of trypan blue staining indicated that radiation-induced cell death was both time and dose dependent process. According to flow-cytometry and DNA fragmentation assay, necrosis was the prevailing form of the radiation-induced cell death in both PC-3 and DU-145 cells. The apoptosis occurred in insignificant number of cells, probably due to the mutant p53 gene present in both cell lines. The cell necrosis was dose dependent and was most pronounced 72 hours post treatment. Conclusion The prevailing form of radiation-induced PC-3 and DU-145 cell death is necrosis. Both PC-3 and DU-145 are rather radioresistant cell lines, as the dose necessary to induce 50% decrease in viable cell number is about 10 Gy.
C3  - Archive of Oncology
T1  - Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells
VL  - 11
IS  - 3
SP  - 197
EP  - 197
DO  - 10.2298/AOO0303197V
ER  - 

@conference{
author = "Vučić, Vesna and Nićiforović, Ana and Adžić, Miroslav and Tišma, Nevena and Janković, Dragana and Ruždijić, Sabera and Radojčić, Marija B.",
year = "2003",
abstract = "Background: Prostate cancer is the first as an incidence and the second as a cause of the oncologic mortality in the male population. There is a broad range of possibilities in the prostate cancer therapy. However, there is also much controversy on the most appropriate treatment in the various stages of the disease. Advanced disease is mostly treated by radiation therapy, sometimes in combination with hormone or chemotherapy. Irradiation induces damage of cell biomolecules, which can lead to the arrest in cell division, or to apoptotic or necrotic cell death. The aim of this study was to determine the dose dependence of radiation-induced cell death in two human prostate cancer cell lines, and to define the form of death of these cells. Methods: Human prostate cancer cell lines PC-3 and DU-145 were irradiated with 2 - 30 Gy from 60 Co g-source, at the dose rate of 20 Gy/h. The effect of irradiation on cell viability, morphology and DNA structure were followed 24 - 72 hours after treatment. Cells were analyzed by trypan blue exclusion assay, flow cytometry and DNA gel electrophoresis. Simultaneous staining of cells with Annexin V-FITC and propidium iodide enabled distinction of early apoptosis from late apoptosis and/or necrosis. Results: The results of trypan blue staining indicated that radiation-induced cell death was both time and dose dependent process. According to flow-cytometry and DNA fragmentation assay, necrosis was the prevailing form of the radiation-induced cell death in both PC-3 and DU-145 cells. The apoptosis occurred in insignificant number of cells, probably due to the mutant p53 gene present in both cell lines. The cell necrosis was dose dependent and was most pronounced 72 hours post treatment. Conclusion The prevailing form of radiation-induced PC-3 and DU-145 cell death is necrosis. Both PC-3 and DU-145 are rather radioresistant cell lines, as the dose necessary to induce 50% decrease in viable cell number is about 10 Gy.",
journal = "Archive of Oncology",
title = "Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells",
volume = "11",
number = "3",
pages = "197-197",
doi = "10.2298/AOO0303197V"
}

Vučić, V., Nićiforović, A., Adžić, M., Tišma, N., Janković, D., Ruždijić, S.,& Radojčić, M. B.. (2003). Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells. in Archive of Oncology, 11(3), 197-197.
https://doi.org/10.2298/AOO0303197V

Vučić V, Nićiforović A, Adžić M, Tišma N, Janković D, Ruždijić S, Radojčić MB. Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells. in Archive of Oncology. 2003;11(3):197-197.
doi:10.2298/AOO0303197V .

Vučić, Vesna, Nićiforović, Ana, Adžić, Miroslav, Tišma, Nevena, Janković, Dragana, Ruždijić, Sabera, Radojčić, Marija B., "Radiation-induced effects in PC-3 and DU-145 human prostate cancer cells" in Archive of Oncology, 11, no. 3 (2003):197-197,
https://doi.org/10.2298/AOO0303197V . .